CRISPOR

DNA break repair outcome predictions

Guide: GGAGATGGCAGTGGGGAGCT AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCCGTGTCGACGGCGACCGCGGAGATGGCAGTGGGGAGCTAGGGTCACCTTGTGATGCGAGCGCTCCTCTGTGCCGCACC`	(Wild-type sequence)
402	`CGGCGACCGCGGAGATGGCAGTGGGG--------TCACCTTGTGATGCGAGCGCTCCTCT`	`8 bp deleted → frameshift`
382	`CGGCGACCGCGGAGATGGCAGTGGG---------TCACCTTGTGATGCGAGCGCTCCTCT`	`9 bp deleted → no frameshift`
282	`CGGCGACCGCGGAGATGGCAGTGGGG-------GTCACCTTGTGATGCGAGCGCTCCTCT`	`7 bp deleted → frameshift`
245	`CGGCGACCGCGGAGATGGCAGTGGGGAG----GGTCACCTTGTGATGCGAGCGCTCCTCT`	`4 bp deleted → frameshift`
242	`CGGCGACCGCGGAGATGGCAGTGG----------TCACCTTGTGATGCGAGCGCTCCTCT`	`10 bp deleted → frameshift`
221	`CGGCGACCGCGGAGATGGCAGTGGGGAGC-----------------------GCTCCTCT`	`23 bp deleted → frameshift`
188	`CGGCGACCGCGGAGATGG---------------GTCACCTTGTGATGCGAGCGCTCCTCT`	`15 bp deleted → no frameshift`
182	`CGGCGACCGCGGAGATGGCAGTGGGGAGCT----------TGTGATGCGAGCGCTCCTCT`	`10 bp deleted → frameshift`
179	`CGGCGACCGCGGAGATGG----------------TCACCTTGTGATGCGAGCGCTCCTCT`	`16 bp deleted → frameshift`
175	`CGGCGACCGCGGAGATGGCAGTG---------------------ATGCGAGCGCTCCTCT`	`21 bp deleted → no frameshift`
173	`CGGCGACCGCGGAGATGGCAG-----------GGTCACCTTGTGATGCGAGCGCTCCTCT`	`11 bp deleted → frameshift`
156	`CGGCGACCGCGGAGATGGCAGT-------------CACCTTGTGATGCGAGCGCTCCTCT`	`13 bp deleted → frameshift`
154	`CGGCGACCGCGGAGATGGCAGTGGGGAGC-------------------GAGCGCTCCTCT`	`19 bp deleted → frameshift`
143	`CGGCGACCGCGGAGATGGCAGTGGGGAGCT-------------------------CCTCT`	`25 bp deleted → frameshift`
140	`CGGCGACCGCGG---------------------GTCACCTTGTGATGCGAGCGCTCCTCT`	`21 bp deleted → no frameshift`
133	`CGGCGACCGCGGAGATG------------------------------CGAGCGCTCCTCT`	`30 bp deleted → no frameshift`
133	`CGGCGACCGCGG----------------------TCACCTTGTGATGCGAGCGCTCCTCT`	`22 bp deleted → frameshift`
128	`CGGCGACCGCGGAGATGGCA-----------------CCTTGTGATGCGAGCGCTCCTCT`	`17 bp deleted → frameshift`
122	`CGGCGACCGCGGAGATGGCAGTGGGGA------------------TGCGAGCGCTCCTCT`	`18 bp deleted → no frameshift`
122	`CGGCGACCGCGGAG------------------GGTCACCTTGTGATGCGAGCGCTCCTCT`	`18 bp deleted → no frameshift`
116	`CGGCGACCGCGGAGATGGCAGTG-------------------TGATGCGAGCGCTCCTCT`	`19 bp deleted → frameshift`
106	`CGGCGACC-------------------------------TTGTGATGCGAGCGCTCCTCT`	`31 bp deleted → frameshift`
94	`CGGCGACCGCGGAGATGGC-----------------------------GAGCGCTCCTCT`	`29 bp deleted → frameshift`
90	`CGGCGACCGCGGAGATGGCAGTG------------------------CGAGCGCTCCTCT`	`24 bp deleted → no frameshift`
90	`CGGCGACCGCG--------------------------------------AGCGCTCCTCT`	`38 bp deleted → frameshift`
89	`CGG------------------------------GTCACCTTGTGATGCGAGCGCTCCTCT`	`30 bp deleted → no frameshift`
86	`CGGCGACCGCGGAGATG-------------------------TGATGCGAGCGCTCCTCT`	`25 bp deleted → frameshift`
84	`CGG-------------------------------TCACCTTGTGATGCGAGCGCTCCTCT`	`31 bp deleted → frameshift`
78	`CGGCGACCGCGGAG-------------------------------------CGCTCCTCT`	`37 bp deleted → frameshift`
77	`CGGCGACCGCGGAGATG---------------------------ATGCGAGCGCTCCTCT`	`27 bp deleted → no frameshift`
77	`CGGCGA--------------------------------------------GCGCTCCTCT`	`44 bp deleted → frameshift`
76	`CGGCGACCGCGGAGATGGC---------------------------------GCTCCTCT`	`33 bp deleted → no frameshift`
74	`CGGCGACCGCGGAGATGGCAGTGGGGAGCT----------------------------CT`	`28 bp deleted → frameshift`
73	`CGGCGACCGCG------------------------------------------CTCCTCT`	`42 bp deleted → no frameshift`
69	`CGGCGACCGCGGAGATGGC-----------------------------------TCCTCT`	`35 bp deleted → frameshift`
66	`CGGCGACCGCGGAGATGGCAGTGGGGAGCT------------------------------`	`30 bp deleted → no frameshift`
66	`CGGCGACCGCGGAGATGGCAG------------------------------CGCTCCTCT`	`30 bp deleted → no frameshift`
66	`CGGCGACCGC--------------------------------------------TCCTCT`	`44 bp deleted → frameshift`
60	`CGGCGACCGCGGA--------------------------------TGCGAGCGCTCCTCT`	`32 bp deleted → frameshift`
54	`CGGCG------------------------------------------------CTCCTCT`	`48 bp deleted → no frameshift`
54	`CGGCGACCG----------------------------------------AGCGCTCCTCT`	`40 bp deleted → frameshift`
52	`CGGCGACCGCGGAGA-----------------------------------GCGCTCCTCT`	`35 bp deleted → frameshift`
42	`CGGCGA---------------------------------------TGCGAGCGCTCCTCT`	`39 bp deleted → no frameshift`
38	`CG-----------------------------------------------AGCGCTCCTCT`	`47 bp deleted → frameshift`
34	`CGGCGACC-------------------------------------------------TCT`	`49 bp deleted → frameshift`
32	`CGGC--------------------------------------------------TCCTCT`	`50 bp deleted → frameshift`
31	`CG---------------------------------------------------CTCCTCT`	`51 bp deleted → no frameshift`

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