← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313617_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313617_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm3_intergenic_Ahr|Agr3_chr12_35616844_F | Primer3: not found at this Tm | N.d. | 0.70 |
| mm3_intergenic_Ahr|Agr3_chr12_35616844_R | Primer3: not found at this Tm | N.d. | 0.70 |
| mm4_intron_Smad3_chr9_63673308_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intron_Smad3_chr9_63673308_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm2_intergenic_Ankrd63|Plcb2_chr2_118706652_F | TCGTCGGCAGCGTCCTTCACACTCCTCCCTCCAC | 59.3 | 0.50 |
| mm2_intergenic_Ankrd63|Plcb2_chr2_118706652_R | GTCTCGTGGGCTCGGGGAGGGGCAGTTACAGACAG | 59.7 | 0.50 |
| mm3_intron_Ccdc91_chr6_147562862_F | Primer3: not found at this Tm | N.d. | 0.48 |
| mm3_intron_Ccdc91_chr6_147562862_R | Primer3: not found at this Tm | N.d. | 0.48 |
| mm4_intergenic_Tm4sf1|Tm4sf4_chr3_57348094_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Tm4sf1|Tm4sf4_chr3_57348094_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm26106|Gm7729_chr18_27425472_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm26106|Gm7729_chr18_27425472_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gabrb2|Gm25543_chr11_42714290_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gabrb2|Gm25543_chr11_42714290_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Cad|Slc30a3_chr5_31083378_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Cad|Slc30a3_chr5_31083378_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intergenic_Gm22768|Ankrd55_chr13_112316354_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm22768|Ankrd55_chr13_112316354_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Eml1|Gm15636_chr12_108429608_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Eml1|Gm15636_chr12_108429608_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intron_Itga9_chr9_118869696_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intron_Itga9_chr9_118869696_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm3_exon_Tenm3_chr8_48646442_F | TCGTCGGCAGCGTCGCTTCGTTTTCCGTATCCGC | 59.9 | 0.32 |
| mm3_exon_Tenm3_chr8_48646442_R | GTCTCGTGGGCTCGGTGTGTGAATCCGCAACTCGA | 59.9 | 0.32 |
| mm4_intergenic_Gm15269|Gm8464_chrX_152476592_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Gm15269|Gm8464_chrX_152476592_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_G930045G22Rik|Mir148a_chr6_51219613_F | TCGTCGGCAGCGTCAGGTTTGGCCAAATGCGTTC | 59.9 | 0.30 |
| mm4_intergenic_G930045G22Rik|Mir148a_chr6_51219613_R | GTCTCGTGGGCTCGGGCAGGGTAAGCTGAAGACGT | 60.0 | 0.30 |
| mm4_intergenic_Gm23752|Gm24815_chr12_6073033_F | TCGTCGGCAGCGTCAGCAAAGATGTGACCAAGAGT | 57.5 | 0.29 |
| mm4_intergenic_Gm23752|Gm24815_chr12_6073033_R | GTCTCGTGGGCTCGGGGGGTTCAGACCACTCTCAG | 59.3 | 0.29 |
| mm4_intron_Zfp345_chr2_150480663_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Zfp345_chr2_150480663_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Gm14139_chr2_150186164_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Gm14139_chr2_150186164_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intron_Dysf_chr6_84014948_F | TCGTCGGCAGCGTCTGTTACAGCTTTATCATTGAGACAA | 57.0 | 0.29 |
| mm4_intron_Dysf_chr6_84014948_R | GTCTCGTGGGCTCGGCCCTGCAGACTTCAGACTGG | 60.0 | 0.29 |
| mm4_intergenic_Zfp773|Zfp418_chr7_7140651_F | TCGTCGGCAGCGTCTCTCAGGCTCCATGATATCACT | 58.1 | 0.28 |
| mm4_intergenic_Zfp773|Zfp418_chr7_7140651_R | GTCTCGTGGGCTCGGTCTGGCATAATCCACTGACATCA | 59.5 | 0.28 |
| mm4_intron_Mylk_chr16_34957964_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Mylk_chr16_34957964_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Zbed6/Zc3h11a|Lax1_chr1_133664757_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Zbed6/Zc3h11a|Lax1_chr1_133664757_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50467579_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50467579_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Rps6ka2_chr17_7276542_F | TCGTCGGCAGCGTCTTAGCTGGACGTTTCTGGGC | 60.3 | 0.26 |
| mm4_intron_Rps6ka2_chr17_7276542_R | GTCTCGTGGGCTCGGCATGCCAGTTCAGTCGGGAT | 60.1 | 0.26 |
| mm4_intergenic_Il1rapl2|Nrk_chrX_138903174_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Il1rapl2|Nrk_chrX_138903174_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Dgkk|Ccnb3_chrX_6975597_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Dgkk|Ccnb3_chrX_6975597_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm3_intergenic_Gm22967|Dlgap1_chr17_69668732_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm3_intergenic_Gm22967|Dlgap1_chr17_69668732_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Dip2a_chr10_76280782_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Dip2a_chr10_76280782_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gm26288|Atp10a_chr7_58262137_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gm26288|Atp10a_chr7_58262137_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_mmu-mir-7243|Gm26846_chr9_102339849_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_mmu-mir-7243|Gm26846_chr9_102339849_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Smoc2_chr17_14301588_F | TCGTCGGCAGCGTCGGCAAGATGGTTTTGTCAGTCT | 59.1 | 0.22 |
| mm4_intron_Smoc2_chr17_14301588_R | GTCTCGTGGGCTCGGAGGCACTCTACTCCTGGGTT | 59.8 | 0.22 |
| mm4_intergenic_Gm4899|Gm5354_chr8_81216839_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm4899|Gm5354_chr8_81216839_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm22673|Gm23059_chr12_59776490_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm22673|Gm23059_chr12_59776490_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Sulf2_chr2_166142264_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Sulf2_chr2_166142264_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Fgf9|Gm17109_chr14_58095835_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Fgf9|Gm17109_chr14_58095835_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Mir466f-4|Irx1_chr13_71144634_F | TCGTCGGCAGCGTCGCCAAGAAGAACAAAGCCGG | 60.0 | 0.20 |
| mm4_intergenic_Mir466f-4|Irx1_chr13_71144634_R | GTCTCGTGGGCTCGGAGGTGGAGGAATTCCCCTGA | 59.8 | 0.20 |
| mm4_intron_Frmd4b_chr6_97368318_F | TCGTCGGCAGCGTCCTCTTGAGCACTGCCTCACA | 59.9 | 0.20 |
| mm4_intron_Frmd4b_chr6_97368318_R | GTCTCGTGGGCTCGGACCGGGTGATAGCTTCCTCT | 60.0 | 0.20 |
| mm4_intron_Slc22a1_chr17_12673921_F | TCGTCGGCAGCGTCGTAACCAGAAGCAGGCCAGG | 60.6 | 0.20 |
| mm4_intron_Slc22a1_chr17_12673921_R | GTCTCGTGGGCTCGGAACGTCAATACAGGCAGGGC | 60.6 | 0.20 |
| mm4_intergenic_Fam49b|Asap1_chr15_64070593_F | TCGTCGGCAGCGTCGCACCTCTCTGTGAAGCACT | 59.9 | 0.18 |
| mm4_intergenic_Fam49b|Asap1_chr15_64070593_R | GTCTCGTGGGCTCGGGGACATTCTCTTTCACAGGGACT | 59.9 | 0.18 |
| mm4_intergenic_Gm23041|Nav3_chr10_109515563_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm23041|Nav3_chr10_109515563_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm22097|Gm23041_chr10_109108180_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm22097|Gm23041_chr10_109108180_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Prap1_chr7_140093953_F | TCGTCGGCAGCGTCTGAGGGTGGTTTGTCCCATG | 59.8 | 0.18 |
| mm4_intron_Prap1_chr7_140093953_R | GTCTCGTGGGCTCGGCCTCGCCTACCTTGCTACTG | 59.8 | 0.18 |
| mm4_intergenic_Sema3d|Gm23243_chr5_12700793_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Sema3d|Gm23243_chr5_12700793_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Stard8_chrX_99004742_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Stard8_chrX_99004742_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_n-R5s61|Gm15571_chr12_68782836_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_n-R5s61|Gm15571_chr12_68782836_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5611|Chordc1_chr9_17496979_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm5611|Chordc1_chr9_17496979_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm3_intergenic_Tfcp2l1/Gm23497|Gli2_chr1_118827308_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intergenic_Tfcp2l1/Gm23497|Gli2_chr1_118827308_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Zfp644|Hfm1_chr5_106709686_F | TCGTCGGCAGCGTCGCTTTAGGGAAGGGCGAGAG | 60.1 | 0.16 |
| mm4_intergenic_Zfp644|Hfm1_chr5_106709686_R | GTCTCGTGGGCTCGGGCTATACTAGGGCGTCAGTGC | 60.3 | 0.16 |
| mm4_intergenic_Gm24692|Sptssa_chr12_54277879_F | TCGTCGGCAGCGTCTGGCAAAGCTCGAACAAACAG | 59.9 | 0.15 |
| mm4_intergenic_Gm24692|Sptssa_chr12_54277879_R | GTCTCGTGGGCTCGGCCCCAGAGCCTTTGTACAGG | 60.0 | 0.15 |
| mm4_intergenic_Rpl30-ps9|Gm14824_chrX_105295506_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Rpl30-ps9|Gm14824_chrX_105295506_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm24393|RP23-452E7.2_chr9_82497117_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm24393|RP23-452E7.2_chr9_82497117_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm15581|Gm25021_chr6_17436116_F | TCGTCGGCAGCGTCCTTTTCTAATGAGGTGAGGACATTT | 57.3 | 0.15 |
| mm4_intergenic_Gm15581|Gm25021_chr6_17436116_R | GTCTCGTGGGCTCGGTTGCACAGCCCTGTAGATCC | 59.7 | 0.15 |
| mm4_intergenic_Gm25715|N6amt1_chr16_87054210_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm25715|N6amt1_chr16_87054210_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_exon_4931406B18Rik_chr7_43495294_F | TCGTCGGCAGCGTCCCCCAGCTGACATACAAAGC | 58.9 | 0.15 |
| mm4_exon_4931406B18Rik_chr7_43495294_R | GTCTCGTGGGCTCGGCTTCAGGCCTAGCACTGTCC | 60.1 | 0.15 |
| mm4_intron_Cdh23_chr10_60598106_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Cdh23_chr10_60598106_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm13603|mmu-mir-6335_chr2_67776523_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm13603|mmu-mir-6335_chr2_67776523_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm20642|Nrg3_chr14_39336537_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm20642|Nrg3_chr14_39336537_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Thsd7a_chr6_12475987_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Thsd7a_chr6_12475987_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Ccdc67|Slc36a4_chr9_15662526_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Ccdc67|Slc36a4_chr9_15662526_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Lrrc37a_chr11_103455112_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Lrrc37a_chr11_103455112_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Gm884_chr11_103540506_F | TCGTCGGCAGCGTCCAGAAGGTGTGGAGGGTGTG | 60.2 | 0.14 |
| mm4_intron_Gm884_chr11_103540506_R | GTCTCGTGGGCTCGGAGCAAAGATCAGCATGGGGT | 59.6 | 0.14 |
| mm3_intron_Atp2b4_chr1_133709845_F | TCGTCGGCAGCGTCAGCTGGTCTGATGAAGGTGC | 60.0 | 0.14 |
| mm3_intron_Atp2b4_chr1_133709845_R | GTCTCGTGGGCTCGGCCCAAGTTCCCTGAGGCTTC | 60.3 | 0.14 |
| mm2_intergenic_Gm11801|Gm22473_chr4_6562232_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm2_intergenic_Gm11801|Gm22473_chr4_6562232_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intron_Ptprf_chr4_118245712_F | TCGTCGGCAGCGTCGACAGACCCTACCAGGCTCT | 60.3 | 0.13 |
| mm3_intron_Ptprf_chr4_118245712_R | GTCTCGTGGGCTCGGGTGCTCCTCACACTGGTGTT | 60.1 | 0.13 |
| mm4_intron_4930593A02Rik_chr3_58789648_F | TCGTCGGCAGCGTCGTAGTCTGAGCACCTGCCAG | 60.1 | 0.13 |
| mm4_intron_4930593A02Rik_chr3_58789648_R | GTCTCGTGGGCTCGGACACCTCACATGGCCACATT | 59.8 | 0.13 |
| mm4_intergenic_Gm9949|Htr4_chr18_62228597_F | TCGTCGGCAGCGTCCTCGCTCCTTTCTCCCCAAG | 60.1 | 0.13 |
| mm4_intergenic_Gm9949|Htr4_chr18_62228597_R | GTCTCGTGGGCTCGGCTTGAGGGTAGTCTAGGTGGC | 59.2 | 0.13 |
| mm4_intron_Fmod_chr1_134044539_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Fmod_chr1_134044539_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Lipg|Rpl17_chr18_74978831_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Lipg|Rpl17_chr18_74978831_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Mier2_chr10_79552671_F | TCGTCGGCAGCGTCGGGACATGGACATGGACCAG | 60.1 | 0.12 |
| mm4_intron_Mier2_chr10_79552671_R | GTCTCGTGGGCTCGGTAGAGAGGGCTGGGCATGAT | 60.1 | 0.12 |
| mm4_intron_Coro1c_chr5_113907997_F | TCGTCGGCAGCGTCGACGGAGAGAAAGCCTGCAT | 60.1 | 0.11 |
| mm4_intron_Coro1c_chr5_113907997_R | GTCTCGTGGGCTCGGCTGCAGGGCTTTCGGAGA | 59.3 | 0.11 |
| mm4_intergenic_Sec16b|Brinp2_chr1_157746750_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Sec16b|Brinp2_chr1_157746750_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_4930465A12Rik_chr4_128152500_F | TCGTCGGCAGCGTCCTTGGTCCCCTGTGCAGTAG | 60.0 | 0.10 |
| mm4_intron_4930465A12Rik_chr4_128152500_R | GTCTCGTGGGCTCGGCAGGACTCCACCCTCTGAGA | 59.9 | 0.10 |
| mm4_exon_E130309D02Rik_chr5_143311873_F | TCGTCGGCAGCGTCTTCAGTGTCTGAACGGAGCC | 59.9 | 0.10 |
| mm4_exon_E130309D02Rik_chr5_143311873_R | GTCTCGTGGGCTCGGCAGCTCAGGGTTCCTCAGTG | 60.0 | 0.10 |
| mm4_exon_Tspan32_chr7_143006970_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_exon_Tspan32_chr7_143006970_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Pid1|Dner_chr1_84366949_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Pid1|Dner_chr1_84366949_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Farp2_chr1_93614555_F | TCGTCGGCAGCGTCATGAACTTAGCAAGGGGCCA | 59.2 | 0.09 |
| mm4_intron_Farp2_chr1_93614555_R | GTCTCGTGGGCTCGGTCTGGAGTGGAGTCAAGGTGA | 60.1 | 0.09 |
| mm4_intergenic_Gpr133|Sfswap_chr5_129320936_F | TCGTCGGCAGCGTCCAGATGTTGGGCACAGAGGT | 59.9 | 0.09 |
| mm4_intergenic_Gpr133|Sfswap_chr5_129320936_R | GTCTCGTGGGCTCGGGGTGGGTGGGAGAGCTTTAG | 59.7 | 0.09 |
| mm4_intergenic_Lrrtm3|Ctnna3_chr10_64182339_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Lrrtm3|Ctnna3_chr10_64182339_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Slc17a8_chr10_89595689_F | TCGTCGGCAGCGTCGCCACAGCTCCAACATCATC | 59.2 | 0.09 |
| mm4_intron_Slc17a8_chr10_89595689_R | GTCTCGTGGGCTCGGCGTCCACTCTGCTCTCTGTG | 60.1 | 0.09 |
| mm4_intron_Fbxo41_chr6_85486974_F | TCGTCGGCAGCGTCTGGTTTTGTGACTTTCTGTGCT | 58.9 | 0.09 |
| mm4_intron_Fbxo41_chr6_85486974_R | GTCTCGTGGGCTCGGAGAACCCATCAGGAGGGAGG | 60.3 | 0.09 |
| mm3_intron_Gnl1_chr17_35984030_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intron_Gnl1_chr17_35984030_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Adra2a|Gpam_chr19_54753038_F | TCGTCGGCAGCGTCCACAAAATCACCAGGGCAGC | 60.0 | 0.09 |
| mm4_intergenic_Adra2a|Gpam_chr19_54753038_R | GTCTCGTGGGCTCGGCCTACTGTGGAAGGTGGCTG | 60.0 | 0.09 |
| mm4_intergenic_Ascl2|Gm15024_chr7_142974808_F | TCGTCGGCAGCGTCCCCTCACTGACACCTTGGAG | 59.6 | 0.09 |
| mm4_intergenic_Ascl2|Gm15024_chr7_142974808_R | GTCTCGTGGGCTCGGATGGCAGCTTGGTCCAATGT | 60.2 | 0.09 |
| mm4_intergenic_RP23-302F9.3|RP23-394M5.2_chr8_87343095_F | TCGTCGGCAGCGTCGCTGGAAAGAGACTGCCGAT | 60.1 | 0.09 |
| mm4_intergenic_RP23-302F9.3|RP23-394M5.2_chr8_87343095_R | GTCTCGTGGGCTCGGCAAGAGAAGGCAGGGGATCC | 59.8 | 0.09 |
| mm4_intergenic_Grpr|Rnf138rt1_chrX_163702399_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Grpr|Rnf138rt1_chrX_163702399_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_2310022B05Rik|Ttc13_chr8_124670827_F | TCGTCGGCAGCGTCCTGAGTTGAGGCAGTGGGAG | 60.0 | 0.08 |
| mm4_intergenic_2310022B05Rik|Ttc13_chr8_124670827_R | GTCTCGTGGGCTCGGGAAGCCCATACCCTGAGAGC | 59.8 | 0.08 |
| mm4_intergenic_Gm14659|Gm14657_chrX_63263463_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm14659|Gm14657_chrX_63263463_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_C030010L15Rik|C030010L15Rik/B230307C23Rik_chr16_97997053_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_C030010L15Rik|C030010L15Rik/B230307C23Rik_chr16_97997053_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Lpgat1|Nek2_chr1_191818432_F | TCGTCGGCAGCGTCCCACACCCGATGACCCCATA | 61.3 | 0.08 |
| mm4_intergenic_Lpgat1|Nek2_chr1_191818432_R | GTCTCGTGGGCTCGGGGGGAGGATTTACAGGACAAA | 57.2 | 0.08 |
| mm4_intergenic_Gfra2|Gm9192_chr14_71424474_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gfra2|Gm9192_chr14_71424474_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_2310022B05Rik_chr8_124636053_F | TCGTCGGCAGCGTCTTGGACACAGCCACCAAGAG | 60.1 | 0.07 |
| mm4_exon_2310022B05Rik_chr8_124636053_R | GTCTCGTGGGCTCGGTGAAGCCGCTGTATGATGCT | 59.8 | 0.07 |
| mm3_intergenic_Fnip2|Gm3513_chr3_79530533_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_Fnip2|Gm3513_chr3_79530533_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Abr_chr11_76507891_F | TCGTCGGCAGCGTCCTCTGCCTAGGCCTCTGTTG | 59.8 | 0.07 |
| mm4_intron_Abr_chr11_76507891_R | GTCTCGTGGGCTCGGCACTGGGGTGGCTGCTATAG | 59.8 | 0.07 |
| mm4_intron_Ext2_chr2_93743381_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Ext2_chr2_93743381_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Tbc1d21|4930461G14Rik_chr9_58433180_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tbc1d21|4930461G14Rik_chr9_58433180_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Tmem38a_chr8_72583150_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Tmem38a_chr8_72583150_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Msi2_chr11_88669338_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Msi2_chr11_88669338_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm11680|BC006965_chr11_112536283_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm11680|BC006965_chr11_112536283_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23891|1700018B24Rik_chr3_47612689_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm23891|1700018B24Rik_chr3_47612689_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_C130071C03Rik/Mir9-2|Gm17750_chr13_83957912_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_C130071C03Rik/Mir9-2|Gm17750_chr13_83957912_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Klhl18_chr9_110428568_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Klhl18_chr9_110428568_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Kcnv1|Gm5472_chr15_45651440_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Kcnv1|Gm5472_chr15_45651440_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm13466|Gm23072_chr2_46264543_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm13466|Gm23072_chr2_46264543_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_4930509K18Rik|Gm23338_chr4_40442952_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_4930509K18Rik|Gm23338_chr4_40442952_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm14751|Rps24-ps3_chrX_79176540_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gm14751|Rps24-ps3_chrX_79176540_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm24957|Gm22492_chr9_112982274_F | TCGTCGGCAGCGTCGATCCCAACTGACCTCTGGC | 60.1 | 0.05 |
| mm4_intergenic_Gm24957|Gm22492_chr9_112982274_R | GTCTCGTGGGCTCGGACATTTGTGGGAGCCTGTGG | 60.5 | 0.05 |
| mm4_intergenic_Agtr1b|Gm7488_chr3_20407308_F | TCGTCGGCAGCGTCACATGTCTGAGCTAAGGAAACA | 57.3 | 0.05 |
| mm4_intergenic_Agtr1b|Gm7488_chr3_20407308_R | GTCTCGTGGGCTCGGTGGTACAGTAAACTCATGGGGA | 58.4 | 0.05 |
| mm4_intergenic_Gm15784|Gm15786_chr6_149382328_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15784|Gm15786_chr6_149382328_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Ppargc1a_chr5_51552689_F | TCGTCGGCAGCGTCTATCACTGCACCACACGGAC | 60.0 | 0.05 |
| mm3_intron_Ppargc1a_chr5_51552689_R | GTCTCGTGGGCTCGGGGAGGATCGGAGCTGCTAAC | 59.9 | 0.05 |
| mm4_intergenic_Gm1862|Gm14591_chrX_53913654_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm1862|Gm14591_chrX_53913654_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15377|Nlrp1a_chr11_71072352_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm15377|Nlrp1a_chr11_71072352_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm13452|Gm13453_chr2_39724687_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm13452|Gm13453_chr2_39724687_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Prdm6_chr18_53468012_F | TCGTCGGCAGCGTCTCCTTCTTTGCTGCTGGGTT | 59.8 | 0.05 |
| mm4_intron_Prdm6_chr18_53468012_R | GTCTCGTGGGCTCGGCATGCCCAATGACGGTCTCT | 60.1 | 0.05 |
| mm4_intergenic_1700016D06Rik|Gm25467_chr8_11689732_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_1700016D06Rik|Gm25467_chr8_11689732_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Prepl_chr17_85076475_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Prepl_chr17_85076475_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Hgd_chr16_37628473_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Hgd_chr16_37628473_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Capn13_chr17_73342938_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Capn13_chr17_73342938_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm12374|Gm12375_chr4_37708026_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm12374|Gm12375_chr4_37708026_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm22016|Cdc73_chr1_142119748_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm22016|Cdc73_chr1_142119748_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_AW209491|Gm6556_chr13_15040157_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_AW209491|Gm6556_chr13_15040157_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_4921524L21Rik|Rab18_chr18_6651735_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_4921524L21Rik|Rab18_chr18_6651735_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Mroh7_chr4_106697154_F | TCGTCGGCAGCGTCGGCTATGGGTCGGGTAGGTA | 60.1 | 0.04 |
| mm4_intron_Mroh7_chr4_106697154_R | GTCTCGTGGGCTCGGGAGGAGGAGGAGGACAGAGG | 60.1 | 0.04 |
| mm4_intron_Wdr27_chr17_14845033_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Wdr27_chr17_14845033_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Fam184b|9630001P10Rik/Fam184b_chr5_45627802_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Fam184b|9630001P10Rik/Fam184b_chr5_45627802_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Nrn1|F13a1_chr13_36817277_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Nrn1|F13a1_chr13_36817277_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Vmn2r101|Vmn2r102_chr17_19638574_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Vmn2r101|Vmn2r102_chr17_19638574_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pip5k1a_chr3_95076145_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pip5k1a_chr3_95076145_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Fezf2_chr14_12344700_F | TCGTCGGCAGCGTCTAAGCGGTGGAGTCCAGGTA | 59.9 | 0.03 |
| mm4_exon_Fezf2_chr14_12344700_R | GTCTCGTGGGCTCGGGCAGGGTCATCAAACCGCAG | 61.9 | 0.03 |
| mm4_intergenic_Ctnna3|Gm22829_chr10_65701226_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ctnna3|Gm22829_chr10_65701226_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm23965|Gm23537_chr1_103512243_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm23965|Gm23537_chr1_103512243_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_L2hgdh_chr12_69717031_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_L2hgdh_chr12_69717031_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Vdac3-ps1|Pou6f2_chr13_18055865_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Vdac3-ps1|Pou6f2_chr13_18055865_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tnks/Gm9939|Rpl31-ps23_chr8_35029913_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tnks/Gm9939|Rpl31-ps23_chr8_35029913_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slitrk4|Gm24598_chrX_64460443_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slitrk4|Gm24598_chrX_64460443_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc47a1|Gm12271_chr11_61394025_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Slc47a1|Gm12271_chr11_61394025_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mkx|Armc4_chr18_7073073_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mkx|Armc4_chr18_7073073_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm7485|Gm14926_chrX_119303022_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm7485|Gm14926_chrX_119303022_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm5121|Ppcdc_chr9_57373120_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm5121|Ppcdc_chr9_57373120_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm25495|Gm23225_chr14_91082050_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm25495|Gm23225_chr14_91082050_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24683|Gm14904_chrX_116150380_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24683|Gm14904_chrX_116150380_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22217|Gm22448_chr16_66164684_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22217|Gm22448_chr16_66164684_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm12570|Olfr319_chr11_58693390_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm12570|Olfr319_chr11_58693390_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_EU599041|Zfp715_chr7_43239030_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_EU599041|Zfp715_chr7_43239030_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_72326618_F | TCGTCGGCAGCGTCGGTGAAGACAAAGGAGCCGA | 59.9 | 0.02 |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_72326618_R | GTCTCGTGGGCTCGGTTCGCCAATGTGTCTAGCCC | 60.3 | 0.02 |
| mm4_intron_Tlr5_chr1_182965209_F | TCGTCGGCAGCGTCCCCTCTGAGCCCTAGACACA | 60.3 | 0.02 |
| mm4_intron_Tlr5_chr1_182965209_R | GTCTCGTGGGCTCGGCCAAGTCAGACATCCCTCGC | 60.4 | 0.02 |
| mm4_intergenic_Gm24014|Spats2l_chr1_57561386_F | TCGTCGGCAGCGTCCCCCTGCCGTTCTTCATAGT | 59.4 | 0.01 |
| mm4_intergenic_Gm24014|Spats2l_chr1_57561386_R | GTCTCGTGGGCTCGGATATTTCACACCGGGGAGCC | 59.8 | 0.01 |
| mm4_intergenic_Gm5893|Gm9844_chr7_24861364_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm5893|Gm9844_chr7_24861364_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Rsph3a|Gm1604A_chr17_8028640_F | TCGTCGGCAGCGTCTAAAGGAGGAACAGTGCCGG | 59.6 | 0.01 |
| mm4_intergenic_Rsph3a|Gm1604A_chr17_8028640_R | GTCTCGTGGGCTCGGCTTCCAGAGTCCAGCCACTG | 60.0 | 0.01 |
| mm4_intergenic_Gm1604b|Rps6ka2_chr17_7097344_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm1604b|Rps6ka2_chr17_7097344_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm23987|Fam135b_chr15_71176769_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm23987|Fam135b_chr15_71176769_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gpr133_chr5_129111648_F | TCGTCGGCAGCGTCGTGCTGAAATCGTCGATGCC | 59.9 | 0.01 |
| mm4_intron_Gpr133_chr5_129111648_R | GTCTCGTGGGCTCGGTGTTCAGCAACACAGACCGA | 59.8 | 0.01 |
| mm4_intron_Setbp1_chr18_78983862_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Setbp1_chr18_78983862_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Lrrtm1|Ctnna2_chr6_77483259_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Lrrtm1|Ctnna2_chr6_77483259_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Wdr61|Crabp1_chr9_54763418_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Wdr61|Crabp1_chr9_54763418_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm14487|Cstad_chr2_30585033_F | TCGTCGGCAGCGTCTTACAGCATGTCCTGGCAGG | 60.0 | 0.01 |
| mm4_intergenic_Gm14487|Cstad_chr2_30585033_R | GTCTCGTGGGCTCGGAAGGGGAGTCACTGACCAGA | 59.8 | 0.01 |
| mm4_intron_Mad1l1_chr5_140163084_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Mad1l1_chr5_140163084_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm15082|Atp2b2_chr6_113940945_F | TCGTCGGCAGCGTCCTCTTGTGGGTGTCTGTGGG | 60.2 | 0.01 |
| mm4_intergenic_Gm15082|Atp2b2_chr6_113940945_R | GTCTCGTGGGCTCGGCTGTGCAATCAGAGTGCCCT | 60.3 | 0.01 |
| mm4_intron_Acot11_chr4_106801608_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Acot11_chr4_106801608_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fam78b|Uck2_chr1_167142756_F | TCGTCGGCAGCGTCCTGGAGAAGGGAGGCTTCTG | 59.4 | 0.00 |
| mm4_intergenic_Fam78b|Uck2_chr1_167142756_R | GTCTCGTGGGCTCGGTCAAGAACCGGTCTCCTTAGC | 59.4 | 0.00 |
| mm4_exon_Tspyl3_chr2_153223362_F | TCGTCGGCAGCGTCTCAAGAACCGGTCTCCTTAGC | 59.4 | 0.00 |
| mm4_exon_Tspyl3_chr2_153223362_R | GTCTCGTGGGCTCGGCTGGAGAAGGGAGGCTTCTG | 59.4 | 0.00 |
| mm4_exon_Iars_chr13_49709602_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Iars_chr13_49709602_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm26404|Gm7799_chr3_37922359_F | TCGTCGGCAGCGTCATGGGAAGCAAGCCCTTTGA | 59.8 | 0.00 |
| mm4_intergenic_Gm26404|Gm7799_chr3_37922359_R | GTCTCGTGGGCTCGGGCTTCTGGCTAGAGGACATCTC | 59.9 | 0.00 |
| mm4_intron_Crocc_chr4_141046359_F | TCGTCGGCAGCGTCAGGCTCCCTGTCTCTCTCTG | 60.0 | 0.00 |
| mm4_intron_Crocc_chr4_141046359_R | GTCTCGTGGGCTCGGGCTGTAAGGAGCTGGAGCAG | 60.4 | 0.00 |
| mm4_intergenic_Spef2|Gm26350_chr15_9773051_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Spef2|Gm26350_chr15_9773051_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gramd4_chr15_86122803_F | TCGTCGGCAGCGTCTTCTCCCAGGTCCAGCCTC | 60.6 | 0.00 |
| mm4_intron_Gramd4_chr15_86122803_R | GTCTCGTGGGCTCGGAGGGCTCTCCATCTAAGCCT | 59.7 | 0.00 |
| mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42255852_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Vmn2r60|Vmn2r61_chr7_42255852_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rapgef1_chr2_29661754_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rapgef1_chr2_29661754_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hsh2d|Cib3_chr8_72202634_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hsh2d|Cib3_chr8_72202634_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Dlgap1|Gm20703_chr17_70825496_F | TCGTCGGCAGCGTCTGCTTGAGCTAACGTGACCA | 59.6 | 0.00 |
| mm4_intergenic_Dlgap1|Gm20703_chr17_70825496_R | GTCTCGTGGGCTCGGGGGCTAAGTTGGACTCCACA | 59.3 | 0.00 |
| mm4_intergenic_Cyp2j7-ps|Gm12658_chr4_96241512_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cyp2j7-ps|Gm12658_chr4_96241512_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Amd-ps4|Gm22563_chr4_51410520_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Amd-ps4|Gm22563_chr4_51410520_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm2_intergenic_Ankrd63|Plcb2_chr2_118706652 |
CTTCACACTCCTCCCTCCACCCGCCCAGCTTCTCCTCCTGCAGGCCCCAGGCTGGCAAAAGGGCTTCCTCCCAGAGGTGC CTCAGTGTGCAAGTCTGTCTGTCTGTAACTGCCCCTCC |
| mm3_exon_Tenm3_chr8_48646442 |
GCTTCGTTTTCCGTATCCGCATCTGACCCTGCACTGATGGAGTAACCTCTGTGAGGGAGCCCCATTTCCGCACAGAATGC CACTCCTCTTCGAGTTGCGGATTCACACA |
| mm4_intergenic_G930045G22Rik|Mir148a_chr6_51219613 |
AGGTTTGGCCAAATGCGTTCATCCCCTGGGAAGAGGCCCCATTCTTCACAATAGCCATGGCTAAAGAGTAACCAGGGAGA AGCGGGCAGTCTTGAGAGGAAACGTCTTCAGCTTACCCTGC |
| mm4_intergenic_Gm23752|Gm24815_chr12_6073033 |
AGCAAAGATGTGACCAAGAGTTTATATAGATATTGTGGCTTCCTCCCAGAGGGGCCTTAGTTCCCCCTCACTCTGAGAGT GGTCTGAACCCC |
| mm4_intron_Dysf_chr6_84014948 |
TGTTACAGCTTTATCATTGAGACAAATCCCCTTGAGAGGAGGCCCCATTGAGTTGAGGCTCCAGTCTGAAGTCTGCAGGG |
| mm4_intergenic_Zfp773|Zfp418_chr7_7140651 |
TCTCAGGCTCCATGATATCACTTATGTTTGTGAACCCCTAGGAGGAAGCCCTTTTGTCTATATTTGGTCCTTGGAAAGTT TCAACAAGCTGATTATGTGAACACATTTAGAAATGATGTCAGTGGATTATGCCAGA |
| mm4_intron_Rps6ka2_chr17_7276542 |
TTAGCTGGACGTTTCTGGGCAGGAGTGGGAGGCTGCCAGAGCCTGACAAGGCCTGGGTCCTTGTTTGAGGTTCCTGCTGT GGGATTTCCTCCCAGAGGTACCTACACTAGAGATGTATATCCCGACTGAACTGGCATG |
| mm4_intron_Smoc2_chr17_14301588 |
GGCAAGATGGTTTTGTCAGTCTACTTCCATTTGGAGGAAGCACCATTTCTTTTAACTAAACAAGCTGCTGAGTACTTCTC ATTGGAGTTAACCCAGGAGTAGAGTGCCT |
| mm4_intergenic_Mir466f-4|Irx1_chr13_71144634 | GCCAAGAAGAACAAAGCCGGAGACCCAAGAGGAGGGGGGCATCCTCCCAGGGGGAACTCAGGGGAATTCCTCCACCT |
| mm4_intron_Frmd4b_chr6_97368318 |
CTCTTGAGCACTGCCTCACACCACCTCCCAGCCTCAGGGTGGAGGCCCCATTGAATCCCCAGGAAGAGGAAGCTATCACC CGGT |
| mm4_intron_Slc22a1_chr17_12673921 | GTAACCAGAAGCAGGCCAGGGGGAAGAGACACTGGGCTTCCTCCCAGAGGCAGCCCTGCCTGTATTGACGTT |
| mm4_intergenic_Fam49b|Asap1_chr15_64070593 |
GCACCTCTCTGTGAAGCACTGGTTGGCAGAGCAGCTCCTGAGACACCCCTGGGAGGAAGATCCAGTGACACTTTCTGTTC CATTTATCATTGAAAGAAGTCCCTGTGAAAGAGAATGTCC |
| mm4_intron_Prap1_chr7_140093953 |
TGAGGGTGGTTTGTCCCATGGGGTCCCTGAGCCAGAGGAACATTAGGTCCTAAATCTCCCCTGGGAAGAAGCAACATTCC AGGCTGTGATGTCCCAAAAGGGACAGTAGCAAGGTAGGCGAGG |
| mm4_intergenic_Zfp644|Hfm1_chr5_106709686 |
GCTTTAGGGAAGGGCGAGAGTATATGGTAGGGAACAGTAGAGGGAACTCTCTCTCTTCACCTATGGGAGGAAGCCACCCT GCTGGGCACTGACGCCCTAGTATAGC |
| mm4_intergenic_Gm24692|Sptssa_chr12_54277879 |
TGGCAAAGCTCGAACAAACAGACTACCCCCAAATTGACAGAAGTGGGGCATCCTCACAGGGGAGCAAACAACATGTAGCA ACAGAGGACGGGCTTTGCTTTACTGTCAACCTGTACAAAGGCTCTGGGG |
| mm4_intergenic_Gm15581|Gm25021_chr6_17436116 |
CTTTTCTAATGAGGTGAGGACATTTATTATTTCAACAGCTGGCAATGGAGCTCCATCTCAGAGGTAAACAACTTAGGGGC AAAGCAGAAATCTCTATGGATCTACAGGGCTGTGCAA |
| mm4_exon_4931406B18Rik_chr7_43495294 | CCCCAGCTGACATACAAAGCATTGACAATGGGGTTTACTCTCAGAGGTCTCCACCGTGGGACAGTGCTAGGCCTGAAG |
| mm4_intron_Gm884_chr11_103540506 |
CAGAAGGTGTGGAGGGTGTGCGGTCTGTGCCCTGGCCAGCGGGCATCCTCCCAGGGGTGTGTAAACAGACCCCATGCTGA TCTTTGCT |
| mm3_intron_Atp2b4_chr1_133709845 |
AGCTGGTCTGATGAAGGTGCCATTTCCCCATGGGAATACTGTACTTTTCCACTCCCCTTTCATAGGCTAGGGGTAGAGAG CTATGGGACTTCCTTCCAGAGGGAAGCCTCAGGGAACTTGGG |
| mm3_intron_Ptprf_chr4_118245712 |
GACAGACCCTACCAGGCTCTGGGGTCAGGGTCCACCCAGCTCACAAACCCACCGAGGCACACAAGCCACTCCAGCCCCAC CACTGTGAGGAAGCCCCATGCCTCGCCAACACCAGTGTGAGGAGCAC |
| mm4_intron_4930593A02Rik_chr3_58789648 |
GTAGTCTGAGCACCTGCCAGAGCTCCTCGCCCACGGGGCACCACCCCCTCCCACTCTCCCAGTAGGAGGAAGCCTCTTTG CTACTTTACTGCAAAGAATCAGCTAATGTGGCCATGTGAGGTGT |
| mm4_intergenic_Gm9949|Htr4_chr18_62228597 |
CTCGCTCCTTTCTCCCCAAGTCTGTTTTGCATGACTGGTCTCTTCTCACCCTTCAGTTGCAATGCTGCCTCCTCACAGAG GGCCACCTAGACTACCCTCAAG |
| mm4_intron_Mier2_chr10_79552671 |
GGGACATGGACATGGACCAGCCCCACAGGCTAACATCGTGAAGCCACAGGGAGGAAGCCCAGGTGCCCTCGGAATCATGC CCAGCCCTCTCTA |
| mm4_intron_Coro1c_chr5_113907997 |
GACGGAGAGAAAGCCTGCATTTCCTTTTCCAGCCTGCAAAAAAAAACCCTTTGAACACTCCCAAAAAAGGACGTCGGGGC CGGCACCTCCCTCCGGGAGGAAGCCCCTTTCTTCTCCGAAAGCCCTGCAG |
| mm4_intron_4930465A12Rik_chr4_128152500 |
CTTGGTCCCCTGTGCAGTAGCATGCATGTGACATTGGGGGTTCCTCACAGGGGTTGTTTGAGGACTCCTGCTGGGCTAGT GCTGTTCTGTTTGGTCTCCCTTCTCTCAGAGGGTGGAGTCCTG |
| mm4_exon_E130309D02Rik_chr5_143311873 |
TTCAGTGTCTGAACGGAGCCCGGCACCAGACAGCAGTAGTCCTCCACAAGCACTCTGGCAAGCCTCACGCAGTAGACCAC GGGCGTCCGCTGGGAAGAAGCCACAGTCCAAGTCACTGAGGAACCCTGAGCTG |
| mm4_intron_Farp2_chr1_93614555 |
ATGAACTTAGCAAGGGGCCACCTCTGGAAGACAGCCCCATTGTTTAATGGTTTACTTTTAAAATATACATTCCTATAGGA AAGCCCTGAAAATACAACATAAATATCACCTTGACTCCACTCCAGA |
| mm4_intergenic_Gpr133|Sfswap_chr5_129320936 |
CAGATGTTGGGCACAGAGGTATGGGACGTGGGGACTCCCTGAAAAGCCAGAAAGAGGTCCAGGTGCAATGGCCCCTCCTC CCACTGGAGCTAAAGCTCTCCCACCCACC |
| mm4_intron_Slc17a8_chr10_89595689 |
GCCACAGCTCCAACATCATCTCCTATCTGCACTCACAGGCACTTGGGCTTCTTCCAAGTGGGACACCCAGCCCTCAGACT ACCACAGAGAGCAGAGTGGACG |
| mm4_intron_Fbxo41_chr6_85486974 |
TGGTTTTGTGACTTTCTGTGCTTAATTGATTCCAACCTCTGTGTGGTAGCCCCATGGCCTCTCTCTGACTCCCCCCCTTC AACTCACACCTTGAAGCTGCTCTCAGCTCCACCCCTCCCTCCTGATGGGTTCT |
| mm4_intergenic_Adra2a|Gpam_chr19_54753038 | CACAAAATCACCAGGGCAGCTTGGAAACCATGGGGCCTCATCCCAGGGGCTCTACAGCCACCTTCCACAGTAGG |
| mm4_intergenic_Ascl2|Gm15024_chr7_142974808 |
CCCTCACTGACACCTTGGAGAGACAGCTTGGTTCCAAGCCATGCCCTGGAAGTGGACAGTGCCACTGGGAAGAAGCCACT TGGCCTGCTAGTAGAAGACACCTTTACCAAATCATCAGACATTGGACCAAGCTGCCAT |
| mm4_intergenic_RP23-302F9.3|RP23-394M5.2_chr8_87343095 |
GCTGGAAAGAGACTGCCGATGCCAATGGGGCTGCCTCTCTGAGGGTGCCATGATCTTCTTGATCTAATGCCATATTGGAG GGGATCCCCTGCCTTCTCTTG |
| mm4_intergenic_2310022B05Rik|Ttc13_chr8_124670827 |
CTGAGTTGAGGCAGTGGGAGCTTGTGAGTCCCCTCCTCAGCTCCCAGCGAGTCCCCTGGTAGGAACCCCCAGCGCCATCA TGCAGCCAGGTGGCCAAGGTAGGGAGTCAGAGAGCTCTCAGGGTATGGGCTTC |
| mm4_intergenic_Lpgat1|Nek2_chr1_191818432 |
CCACACCCGATGACCCCATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNAGCCAACCATTTCCCACTTTGGTAATTACTGATTTGTCCTGTAAATCCTCCCC |
| mm4_exon_2310022B05Rik_chr8_124636053 |
TTGGACACAGCCACCAAGAGCAGGACACAGAGCCCTGGAGCTGCCTCCCAGAGGTGCTGAGCCTGGGCATGTGGCGGAAC CCAGCATCATACAGCGGCTTCA |
| mm4_intron_Abr_chr11_76507891 |
CTCTGCCTAGGCCTCTGTTGCTATGGCAACAATGTGGCTTCCCCCAAGTGGGATGCTGGTTGCCAAGGCCCTTGTTGCCA AAGGCTATAGCAGCCACCCCAGTG |
| mm4_intergenic_Gm24957|Gm22492_chr9_112982274 |
GATCCCAACTGACCTCTGGCCTCCACTGGCAAGCAGCTCCATTGCACGTCTTCCTCCAGCCCACAGGCTCCCACAAATGT |
| mm4_intergenic_Agtr1b|Gm7488_chr3_20407308 |
ACATGTCTGAGCTAAGGAAACATCTTGGAATGGGGCATCATTCCAGTGGATAATCCTGTACATACAAGCCTATGTACATT CAAAATGCATTGCTTCTATCCTTGTATGGTTCCCCATGAGTTTACTGTACCA |
| mm3_intron_Ppargc1a_chr5_51552689 |
TATCACTGCACCACACGGACATCTTATTTAATCAAATCACGTGTGGCACTTGACCCTCCCCCTTACCCTACGTGCCCCCG CTACCGGGAGGAAGCACCATTGTTAGTTAGCAGCTCCGATCCTCC |
| mm4_intron_Prdm6_chr18_53468012 |
TCCTTCTTTGCTGCTGGGTTGCCTGAACCCATGGGGCTGCATCCCAGTGGTCTCTTCTCCAGTTCTGGGCAGCTGAAGTG TTTTCTGAGTCCTTATTAGTACTCCTGGAGGATGCTGAGAGACCGTCATTGGGCATG |
| mm4_intron_Mroh7_chr4_106697154 |
GGCTATGGGTCGGGTAGGTAGGCACTCTGGCTTCCTTCCAGTGGCTCTGTATCTTAGCTGAGATAAGACCTCTCTCTTGG AGGTTATGACCTTCCCCACTCCAAGGACAGGAGGCTTCCCTCTGTCCTCCTCCTCCTC |
| mm4_exon_Fezf2_chr14_12344700 |
TAAGCGGTGGAGTCCAGGTAGTTGAAGTAGTAGAGAGAGCCGCTGGCAGGCAGCCCCACAGCCTGGTTGATGACCTGCGG TTTGATGACCCTGC |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_72326618 | GGTGAAGACAAAGGAGCCGATCCTCTGTGAGCAAACCCCATGGCCTGGGCTAGACACATTGGCGAA |
| mm4_intron_Tlr5_chr1_182965209 |
CCCTCTGAGCCCTAGACACAAGTTAACCCAGCCACAATAATCAAAACCCAGTGCCCAGCTTTCTCCAGCCTTCTCACCGG AGCTTCCTCCCAGAGAAGCGAGGGATGTCTGACTTGG |
| mm4_intergenic_Gm24014|Spats2l_chr1_57561386 | CCCCTGCCGTTCTTCATAGTGCAATGGGGCTTGCCCCAAATGGGCATGGCTCCCCGGTGTGAAATAT |
| mm4_intergenic_Rsph3a|Gm1604A_chr17_8028640 |
TAAAGGAGGAACAGTGCCGGGGCCTCTGTATCCAGGGACTTACAGGAGAAGCTGCGCTGGGGCTGCCTGCCAGAGGATCG GAGTCACGCCTGGGAGAGACAGTGGCTGGACTCTGGAAG |
| mm4_intron_Gpr133_chr5_129111648 |
GTGCTGAAATCGTCGATGCCTCCACGTCTGTCCCCACGCTGTGCAATGTGGCTTCCTGTCGGAGGCCCCCTCTTCGGTCT GTGTTGCTGAACA |
| mm4_intergenic_Gm14487|Cstad_chr2_30585033 |
TTACAGCATGTCCTGGCAGGGATAGCCTCATCCAAAAGGCAGGAGGTCCCCTGGCAGGACCACCCATTGCTTTGACACCC AGTATCTGGTCAGTGACTCCCCTT |
| mm4_intergenic_Gm15082|Atp2b2_chr6_113940945 | CTCTTGTGGGTGTCTGTGGGGCCCCAGTCAGCCTCTGACAGGAAGCCCCAGGGCAGGGCACTCTGATTGCACAG |
| mm4_intergenic_Fam78b|Uck2_chr1_167142756 |
CTGGAGAAGGGAGGCTTCTGGCCATGGGGCTTGCTTCCACTGGCAGAGATATCACAAGGCTTTTAATGGAACAACGCTGA AACATATCTGAAGATGCTATTTGTTTCCCCAAATAGCTAAGGAGACCGGTTCTTGA |
| mm4_exon_Tspyl3_chr2_153223362 |
TCAAGAACCGGTCTCCTTAGCTATTTGGGGAAACAAATAGCATCTTCAGATATGTTTCAGCGTTGTTCCATTAAAAGCCA TGTGATATCTCTGCCAGTGGAAGCAAGCCCCATGGCCAGAAGCCTCCCTTCTCCAG |
| mm4_intergenic_Gm26404|Gm7799_chr3_37922359 |
ATGGGAAGCAAGCCCTTTGAGAGTCATCTGGTCACTGGGAGGAATCCCCAGGGGCTAGTTTAGTACCCTCATATAAGATG CCTCAGAGATGTCCTCTAGCCAGAAGC |
| mm4_intron_Crocc_chr4_141046359 |
AGGCTCCCTGTCTCTCTCTGGACTTGACTGAACCCCTGCAAGGACGCCCCACTGCCCTGCTCACCTCTTCCTCGCGCCTC CACACTGCCTCCCGCTGCTCCAGCTCCTTACAGC |
| mm4_intron_Gramd4_chr15_86122803 |
TTCTCCCAGGTCCAGCCTCACTCCACCCACTGGCAGCCAGCCCCAGTGCAGGTCTGGCAGCTGCATCCCCTGGGAGGTGG CCTCCTCGGGCCATCCGGAGGATCCCTGGCCAGTTCCCAGGCTTAGATGGAGAGCCCT |
| mm4_intergenic_Dlgap1|Gm20703_chr17_70825496 |
TGCTTGAGCTAACGTGACCAAGTGAGCAATGGGGGTTGGTCCCAAAGGCTGCTCTTGAGTGAAGGCAAAATGGATAGTTG GAATGATTCAACCTTGGATTCCTGTGGAGTCCAACTTAGCCC |