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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| AACCATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCC | (Wild-type sequence) | |
| 219 | TTTCGGCTCTCCATCCCCGG------------GGGGGAGGGGGAAAACCCCCAAGTAAGC | 12 bp deleted → no frameshift |
| 213 | TTTCGGCTCTCCATCCCCGGA-----------------GGGGGAAAACCCCCAAGTAAGC | 17 bp deleted → frameshift |
| 208 | TTTCGGCTCTCCATCCCCGG-------------GGGGAGGGGGAAAACCCCCAAGTAAGC | 13 bp deleted → frameshift |
| 201 | TTTCGGCTCTCCATCCCCGGACTCTCCAGA--------GGGGGAAAACCCCCAAGTAAGC | 8 bp deleted → frameshift |
| 198 | TTTCGGCTCTCCATCCCCGG--------------GGGAGGGGGAAAACCCCCAAGTAAGC | 14 bp deleted → frameshift |
| 188 | TTTCGGCTCTCCATCCCCGG---------------GGAGGGGGAAAACCCCCAAGTAAGC | 15 bp deleted → no frameshift |
| 182 | TTTCGGCTCTCCATCCCCGGACTCTCCAG----------GGGGAAAACCCCCAAGTAAGC | 10 bp deleted → frameshift |
| 179 | TTTCGGCTCTCCATCCCCGG----------------GAGGGGGAAAACCCCCAAGTAAGC | 16 bp deleted → frameshift |
| 158 | TTTCGGCTCTCCATCCCCGGA-----------------------AAACCCCCAAGTAAGC | 23 bp deleted → frameshift |
| 153 | TTTCGGCTCTCCATCCCC---------------------------------CAAGTAAGC | 33 bp deleted → no frameshift |
| 149 | TTTCGGCTCTCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGC | 14 bp deleted → frameshift |
| 147 | TTTCGGCTCTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAGC | 20 bp deleted → frameshift |
| 146 | TTTCGGCTCTCCATCCCC----------------------------------AAGTAAGC | 34 bp deleted → frameshift |
| 143 | TTTCGGCTCTCCATCCCCGGACTCTCCA-------------------------AGTAAGC | 25 bp deleted → frameshift |
| 140 | TTTCGGCTCTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGC | 21 bp deleted → no frameshift |
| 133 | TTTCGGCTCTCCATCCCCGGACTCTCC----------------------CCCAAGTAAGC | 22 bp deleted → frameshift |
| 133 | TTTCGGCTCTCCATCCCCGG----------------------GAAAACCCCCAAGTAAGC | 22 bp deleted → frameshift |
| 126 | TTTCGGCTCTCCATCCCCGGACTCTCC-----------------------CCAAGTAAGC | 23 bp deleted → frameshift |
| 121 | TTTCGGCTCTCCATCCCC--------------------------------CCAAGTAAGC | 32 bp deleted → frameshift |
| 120 | TTTCGGCTCTCCATCCCCGGACTCTCC------------------------CAAGTAAGC | 24 bp deleted → no frameshift |
| 109 | TTTCGG--------------------------GGGGGAGGGGGAAAACCCCCAAGTAAGC | 26 bp deleted → frameshift |
| 104 | TTTCGGCTCTCCATCCC-----------------------------------AAGTAAGC | 35 bp deleted → frameshift |
| 103 | TTTCGG---------------------------GGGGAGGGGGAAAACCCCCAAGTAAGC | 27 bp deleted → no frameshift |
| 98 | TTTCGG----------------------------GGGAGGGGGAAAACCCCCAAGTAAGC | 28 bp deleted → frameshift |
| 94 | TTTCGG-----------------------------GGAGGGGGAAAACCCCCAAGTAAGC | 29 bp deleted → frameshift |
| 89 | TTTCGG------------------------------GAGGGGGAAAACCCCCAAGTAAGC | 30 bp deleted → no frameshift |
| 84 | TTTCGGCTCTCCATCCCC-------------------------------CCCAAGTAAGC | 31 bp deleted → frameshift |
| 84 | TTTCGG-------------------------------AGGGGGAAAACCCCCAAGTAAGC | 31 bp deleted → frameshift |
| 81 | TTTCGGCTCTCCATCCCCGGACTCTCCAG--------------------------TAAGC | 26 bp deleted → frameshift |
| 81 | TTTCGGCTCTCCATCCCCGGAC--------------------------CCCCAAGTAAGC | 26 bp deleted → frameshift |
| 73 | TTTCGG----------------------------------GGGAAAACCCCCAAGTAAGC | 34 bp deleted → frameshift |
| 69 | TTTCGG-----------------------------------GGAAAACCCCCAAGTAAGC | 35 bp deleted → frameshift |
| 67 | TTTCGGCTCTCCA----------------------------------------AGTAAGC | 40 bp deleted → frameshift |
| 66 | TTTCGGCTCTCCATCCCCGGACTCTCCAG------------------------------C | 30 bp deleted → no frameshift |
| 66 | TTTCGGCTCTCCATCC------------------------------------AAGTAAGC | 36 bp deleted → no frameshift |
| 66 | TTTCGG------------------------------------GAAAACCCCCAAGTAAGC | 36 bp deleted → no frameshift |
| 62 | TTTCGGCTCTCC-------------------------------------CCCAAGTAAGC | 37 bp deleted → frameshift |
| 62 | TTTCGG-------------------------------------AAAACCCCCAAGTAAGC | 37 bp deleted → frameshift |
| 60 | TTTCGGCTCTCC--------------------------------------CCAAGTAAGC | 38 bp deleted → frameshift |
| 56 | TTTCGGCTCTCC---------------------------------------CAAGTAAGC | 39 bp deleted → no frameshift |
| 28 | TTTCGGC----------------------------------------------------- | 53 bp deleted → frameshift |