CRISPOR

DNA break repair outcome predictions

Guide: CTGTCCGCGGAGGGGACTGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCGGGCCAAGCATGGCCGCTCCAGCAGACCGTGGC`	(Wild-type sequence)
402	`GCCAGAGAGGCTGTCCGCGGAGGGG--------TCCCGGGCCAAGCATGGCCGCTCCAGC`	`8 bp deleted → frameshift`
382	`GCCAGAGAGGCTGTCCGCGGAGGG---------TCCCGGGCCAAGCATGGCCGCTCCAGC`	`9 bp deleted → no frameshift`
362	`GCCAGAGAGGCTGTCCGCGGAGGGGACTGG--GTCCCGGGCCAAGCATGGCCGCTCCAGC`	`2 bp deleted → frameshift`
344	`GCCAGAGAGGCTGTCCGCGGAGGGGACTGG---TCCCGGGCCAAGCATGGCCGCTCCAGC`	`3 bp deleted → no frameshift`
283	`GCCAGAGAGGCTGTCCGCGGAGGGG---------------CCAAGCATGGCCGCTCCAGC`	`15 bp deleted → no frameshift`
282	`GCCAGAGAGGCTGTCCGCGGAGGGG-------GTCCCGGGCCAAGCATGGCCGCTCCAGC`	`7 bp deleted → frameshift`
269	`GCCAGAGAGGCTGTCCGCGGAGGG----------------CCAAGCATGGCCGCTCCAGC`	`16 bp deleted → frameshift`
257	`GCCAGAGAGGCTGTCC--------------------CGGGCCAAGCATGGCCGCTCCAGC`	`20 bp deleted → frameshift`
255	`GCCAGAGAGGCTGTCCGCGGAGGGGACTGG---------GCCAAGCATGGCCGCTCCAGC`	`9 bp deleted → no frameshift`
242	`GCCAGAGAGGCTGTCCGCGGAGGGGACTGG----------CCAAGCATGGCCGCTCCAGC`	`10 bp deleted → frameshift`
242	`GCCAGAGAGGCTGTCCGCGGAGG----------TCCCGGGCCAAGCATGGCCGCTCCAGC`	`10 bp deleted → frameshift`
232	`GCCAGAGAGGCTGTCCGCGG-------------------GCCAAGCATGGCCGCTCCAGC`	`19 bp deleted → frameshift`
219	`GCCAGAGAGGCTGTCCGCGG------------GTCCCGGGCCAAGCATGGCCGCTCCAGC`	`12 bp deleted → no frameshift`
210	`GCCAGAGAGGCTGTCCG---------------------GGCCAAGCATGGCCGCTCCAGC`	`21 bp deleted → no frameshift`
208	`GCCAGAGAGGCTGTCCGCGG-------------TCCCGGGCCAAGCATGGCCGCTCCAGC`	`13 bp deleted → frameshift`
198	`GCCAGAGAGGCTGTCCGCGGAGGGG--------------GCCAAGCATGGCCGCTCCAGC`	`14 bp deleted → frameshift`
184	`GCCAGAGAGGCTGTCCGCGGAGGGGACTGG--------------------CCGCTCCAGC`	`20 bp deleted → frameshift`
170	`GCCAGAGAGGCTGTCCGCGGAGG-----------------CCAAGCATGGCCGCTCCAGC`	`17 bp deleted → frameshift`
147	`GCCAGAGAGGCTGTCCGCGG--------------------CCAAGCATGGCCGCTCCAGC`	`20 bp deleted → frameshift`
133	`GCCAGAGAGGC------------------------------CAAGCATGGCCGCTCCAGC`	`30 bp deleted → no frameshift`
133	`GCCAGAGAGG----------------------GTCCCGGGCCAAGCATGGCCGCTCCAGC`	`22 bp deleted → frameshift`
132	`GCCAGAGAGGCTGTCCGC------------------------------------TCCAGC`	`36 bp deleted → no frameshift`
126	`GCCAGAGAGGCTGTCCGC-----------------------CAAGCATGGCCGCTCCAGC`	`23 bp deleted → frameshift`
126	`GCCAGAGAGG-----------------------TCCCGGGCCAAGCATGGCCGCTCCAGC`	`23 bp deleted → frameshift`
114	`GCCAGAGAGGCTGTCCGCGGAGGGG-------------------------CCGCTCCAGC`	`25 bp deleted → frameshift`
109	`GCCAGAGAGGCTGTCCGCGGAGGG--------------------------CCGCTCCAGC`	`26 bp deleted → frameshift`
109	`GCCAGAGAGGCTGTCC--------------------------AAGCATGGCCGCTCCAGC`	`26 bp deleted → frameshift`
103	`GCCAGAGAGGCTGTCCGCGGAGG---------------------------CCGCTCCAGC`	`27 bp deleted → no frameshift`
99	`GCCA---------------------------------------AGCATGGCCGCTCCAGC`	`39 bp deleted → no frameshift`
98	`GCCAGAGAGGCTGTCCGC----------------------------ATGGCCGCTCCAGC`	`28 bp deleted → frameshift`
95	`GCCAGAGAGGCTGTCCGCGGAG-----------------------CATGGCCGCTCCAGC`	`23 bp deleted → frameshift`
94	`GCCAGAGAGG-----------------------------GCCAAGCATGGCCGCTCCAGC`	`29 bp deleted → frameshift`
89	`GCCAGAGAGGCTGTCCGCGG------------------------------CCGCTCCAGC`	`30 bp deleted → no frameshift`
81	`GCCAGAGAGGC----------------------------------------CGCTCCAGC`	`40 bp deleted → frameshift`
77	`GCCAGAGAGGCTGTCCGCGGAGGGGACT---------------------------CCAGC`	`27 bp deleted → no frameshift`
76	`GCCAGAGAGGCTGTCCGC---------------------------------CGCTCCAGC`	`33 bp deleted → no frameshift`
76	`GCC---------------------------------CGGGCCAAGCATGGCCGCTCCAGC`	`33 bp deleted → no frameshift`
73	`GCCAGAGAGGCTGTCCGCG----------------------------------CTCCAGC`	`34 bp deleted → frameshift`
73	`GCC----------------------------------GGGCCAAGCATGGCCGCTCCAGC`	`34 bp deleted → frameshift`
69	`GCCAGAGAGGC-----------------------------------ATGGCCGCTCCAGC`	`35 bp deleted → frameshift`
64	`GCCAGAGAGGCTGTCC-----------------------------------------AGC`	`41 bp deleted → frameshift`
58	`GCCAGAGAGGCT-------------------------------------------CCAGC`	`43 bp deleted → frameshift`
51	`GCC-------------------------------------------------GCTCCAGC`	`49 bp deleted → frameshift`
49	`GCCAGAGAGGCTG------------------------------------GCCGCTCCAGC`	`36 bp deleted → no frameshift`
49	`GCCAGAGAG------------------------------------CATGGCCGCTCCAGC`	`36 bp deleted → no frameshift`
48	`GCCAGAGAGGCTGTCCGC------------------------------------------`	`42 bp deleted → no frameshift`
47	`GCCAGAGAGGCTGTCCGCGGAG-------------------------------------C`	`37 bp deleted → frameshift`
46	`GCCAG------------------------------------------------------C`	`54 bp deleted → no frameshift`
45	`GCCAGAG--------------------------------------CATGGCCGCTCCAGC`	`38 bp deleted → frameshift`
44	`GC--------------------------------------------ATGGCCGCTCCAGC`	`44 bp deleted → frameshift`
40	`GCCAG----------------------------------------CATGGCCGCTCCAGC`	`40 bp deleted → frameshift`
34	`GCCA-------------------------------------------TGGCCGCTCCAGC`	`43 bp deleted → frameshift`
34	`GCCAGAGAGGC-------------------------------------------------`	`49 bp deleted → frameshift`
29	`GC----------------------------------------------------TCCAGC`	`52 bp deleted → frameshift`
24	`GCCAGAGAG--------------------------------------------------C`	`50 bp deleted → frameshift`
22	`GCCAGAG----------------------------------------------------C`	`52 bp deleted → frameshift`
22	`GC----------------------------------------------------------`	`58 bp deleted → frameshift`

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