CRISPOR

DNA break repair outcome predictions

Guide: GACACGGCGCTGGGGCTACC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTGCCTTGTCGCCTTAGCTCCTCTTCTCAGCCAA`	(Wild-type sequence)
538	`GGTCGCCGTCGACACGGCGC----------------CTGCCTTGTCGCCTTAGCTCCTCT`	`16 bp deleted → frameshift`
402	`GGTCGCCGTCGACACGGCGCTGGGGC--------GCCTGCCTTGTCGCCTTAGCTCCTCT`	`8 bp deleted → frameshift`
282	`GGTCGCCGTCGACACGGCGCTGGGGCTACC-------TGCCTTGTCGCCTTAGCTCCTCT`	`7 bp deleted → frameshift`
255	`GGTCGCCGTCGACACGGCGCTGGG---------CGCCTGCCTTGTCGCCTTAGCTCCTCT`	`9 bp deleted → no frameshift`
242	`GGTCGCCGTCGACACGGCGCTGGGGC----------CTGCCTTGTCGCCTTAGCTCCTCT`	`10 bp deleted → frameshift`
242	`GGTCGCCGTCGACACGGCGCTGG----------CGCCTGCCTTGTCGCCTTAGCTCCTCT`	`10 bp deleted → frameshift`
230	`GGTCGCCGTCGACACGGCGCTGGGGCTACC-----------TTGTCGCCTTAGCTCCTCT`	`11 bp deleted → frameshift`
213	`GGTCGCCGTCGACACGGCGCTG-----------------CCTTGTCGCCTTAGCTCCTCT`	`17 bp deleted → frameshift`
208	`GGTCGCCGTCGACACGGCG-------------GCGCCTGCCTTGTCGCCTTAGCTCCTCT`	`13 bp deleted → frameshift`
198	`GGTCGCCGTCGACACGGCGCTGGGGC--------------CTTGTCGCCTTAGCTCCTCT`	`14 bp deleted → frameshift`
198	`GGTCGCCGTCGACACGGCGC--------------GCCTGCCTTGTCGCCTTAGCTCCTCT`	`14 bp deleted → frameshift`
178	`GGTCGCC------------------------------TGCCTTGTCGCCTTAGCTCCTCT`	`30 bp deleted → no frameshift`
173	`GGTCGCCGTCGACACGGCGCTGGGGCT-----------GCCTTGTCGCCTTAGCTCCTCT`	`11 bp deleted → frameshift`
162	`GGTCGCCGTCGACACGGC------------------CTGCCTTGTCGCCTTAGCTCCTCT`	`18 bp deleted → no frameshift`
154	`GGTCGCCGTCGACACGGCGCTGGGGCTACC-------------------TTAGCTCCTCT`	`19 bp deleted → frameshift`
154	`GGTCGCCGTCGACACG-------------------CCTGCCTTGTCGCCTTAGCTCCTCT`	`19 bp deleted → frameshift`
148	`GGTCGCCGTCGACACGGCGC----------------------------CTTAGCTCCTCT`	`28 bp deleted → frameshift`
147	`GGTCGCCGTCGACACGGCGC--------------------CTTGTCGCCTTAGCTCCTCT`	`20 bp deleted → frameshift`
141	`GGTCGCCGTCGACACGGCGCTGGGGCT---------------TGTCGCCTTAGCTCCTCT`	`15 bp deleted → no frameshift`
140	`GGTCGCCGTCG---------------------GCGCCTGCCTTGTCGCCTTAGCTCCTCT`	`21 bp deleted → no frameshift`
134	`GGTCGCC------------------------------------------TTAGCTCCTCT`	`42 bp deleted → no frameshift`
133	`GGTCGCCGTCGACACGGCGCTGGGGC----------------------CTTAGCTCCTCT`	`22 bp deleted → frameshift`
133	`GGTCGCCGTCGACACGGC----------------------CTTGTCGCCTTAGCTCCTCT`	`22 bp deleted → frameshift`
123	`GGTCGCCGTCGACACGGCGCTGGGGCT----------------------------CCTCT`	`28 bp deleted → frameshift`
120	`GGTCGCCGTCG------------------------CCTGCCTTGTCGCCTTAGCTCCTCT`	`24 bp deleted → no frameshift`
120	`GGTCGCCG------------------------GCGCCTGCCTTGTCGCCTTAGCTCCTCT`	`24 bp deleted → no frameshift`
115	`GGTCGCCGTCG------------------------------------CCTTAGCTCCTCT`	`36 bp deleted → no frameshift`
109	`GGTCGCC----------------------------------TTGTCGCCTTAGCTCCTCT`	`34 bp deleted → frameshift`
105	`GGTCGCCGTCGACACGGCGCT---------------------TGTCGCCTTAGCTCCTCT`	`21 bp deleted → no frameshift`
103	`GGTCGCCGTCGACACGGCGCTGGGGCTACC---------------------------TCT`	`27 bp deleted → no frameshift`
103	`GGTCGCCG---------------------------CCTGCCTTGTCGCCTTAGCTCCTCT`	`27 bp deleted → no frameshift`
103	`GGTCG---------------------------GCGCCTGCCTTGTCGCCTTAGCTCCTCT`	`27 bp deleted → no frameshift`
99	`GGTCGCCGTCGACACGGCGCTG----------------------TCGCCTTAGCTCCTCT`	`22 bp deleted → frameshift`
98	`GGTCGC----------------------------GCCTGCCTTGTCGCCTTAGCTCCTCT`	`28 bp deleted → frameshift`
95	`GGTCGCCGTCGACACGGCGCTGGGGCT-----------------------TAGCTCCTCT`	`23 bp deleted → frameshift`
91	`GGTCGCCGTCGACACGGCGCT----------------------------------CCTCT`	`34 bp deleted → frameshift`
89	`GGTCGCCGTCGACACGGC------------------------------CTTAGCTCCTCT`	`30 bp deleted → no frameshift`
84	`GGTCGCCGTCGACACG-------------------------------CCTTAGCTCCTCT`	`31 bp deleted → frameshift`
84	`GG-------------------------------CGCCTGCCTTGTCGCCTTAGCTCCTCT`	`31 bp deleted → frameshift`
70	`GGTCGCCGTCGACACGGCGCT-----------------------------TAGCTCCTCT`	`29 bp deleted → frameshift`
66	`GGTCGCCGTCGACACGGC------------------------------------TCCTCT`	`36 bp deleted → no frameshift`
63	`GGTCGCCGTCGACACGGCGCTGGGGCT-------------------------------CT`	`31 bp deleted → frameshift`
60	`GGTCGCCGTCGACACGGCGCTGGGGCTA------------------------GCTCCTCT`	`24 bp deleted → no frameshift`
57	`GGTCGCCGTCGACACGGCGCTGGGGCT---------------------------------`	`33 bp deleted → no frameshift`
56	`GGTCGCCG---------------------------------------CCTTAGCTCCTCT`	`39 bp deleted → no frameshift`
47	`GGTCGCCGTCGACACGGCGCT-------------------------------------CT`	`37 bp deleted → frameshift`
42	`GGTCGCCGTCGACACGGCGCT---------------------------------------`	`39 bp deleted → no frameshift`
36	`GGTCGC------------------------------------------------TCCTCT`	`48 bp deleted → no frameshift`
32	`GGTCGCC--------------------------------------------------TCT`	`50 bp deleted → frameshift`
30	`GGTCGCCGTC----------------------------------------------CTCT`	`46 bp deleted → frameshift`
25	`GGTCGCCGTC-------------------------------------------------T`	`49 bp deleted → frameshift`
22	`GGTC----------------------------------------------------CTCT`	`52 bp deleted → frameshift`
19	`GGTC-------------------------------------------------------T`	`55 bp deleted → frameshift`

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