CRISPOR

DNA break repair outcome predictions

Guide: CTCCGCGGTCGCCGTCGACA CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCCTAGCTCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTGCCTTGTCGCCTTAGC`	(Wild-type sequence)
311	`CCACTGCCATCTCCGCGGTCGCCGTCG-----GCGCTGGGGCTACCCGGCGCCTGCCTTG`	`5 bp deleted → frameshift`
298	`CCACTGCCATCTCCGCGGTCGC--------------TGGGGCTACCCGGCGCCTGCCTTG`	`14 bp deleted → frameshift`
283	`CCACTGCCATCTCCGCGG---------------CGCTGGGGCTACCCGGCGCCTGCCTTG`	`15 bp deleted → no frameshift`
268	`CCACTGCCATCTCCGCGGTCGCCGTCG--------CTGGGGCTACCCGGCGCCTGCCTTG`	`8 bp deleted → frameshift`
268	`CCACTGCCATCTCCGCGGTCGCCG--------GCGCTGGGGCTACCCGGCGCCTGCCTTG`	`8 bp deleted → frameshift`
244	`CCACTGCCATCTCCGCG------------------CTGGGGCTACCCGGCGCCTGCCTTG`	`18 bp deleted → no frameshift`
230	`CCACTGCCATCTCCGCGGTCGCCG-----------CTGGGGCTACCCGGCGCCTGCCTTG`	`11 bp deleted → frameshift`
230	`CCACTGCCATCTCCGCGGTCG-----------GCGCTGGGGCTACCCGGCGCCTGCCTTG`	`11 bp deleted → frameshift`
220	`CCACTGCCATCTCCGC--------------------TGGGGCTACCCGGCGCCTGCCTTG`	`20 bp deleted → frameshift`
219	`CCACTGCCATCTCCGCGGTCGC------------GCTGGGGCTACCCGGCGCCTGCCTTG`	`12 bp deleted → no frameshift`
180	`CCACTGCCATCTCCGCGGTCGCCG------------------------GCGCCTGCCTTG`	`24 bp deleted → no frameshift`
178	`CCACTGCCATCTCCGCGGTCGCC------------------------------TGCCTTG`	`30 bp deleted → no frameshift`
170	`CCACTGCCATCTCCG-----------------GCGCTGGGGCTACCCGGCGCCTGCCTTG`	`17 bp deleted → frameshift`
147	`CCACTGCCATCTCCGCGGTCGC--------------------TACCCGGCGCCTGCCTTG`	`20 bp deleted → frameshift`
140	`CCACTGCCATCTCCGCGGTCGCCGTCG---------------------GCGCCTGCCTTG`	`21 bp deleted → no frameshift`
140	`CCACTGCCATCTCCGCGG---------------------GGCTACCCGGCGCCTGCCTTG`	`21 bp deleted → no frameshift`
134	`CCACTGCCATCTCCGCGGTCGCCGTCGAC----------------CCGGCGCCTGCCTTG`	`16 bp deleted → frameshift`
133	`CCACTGCCATCTCCGCGG----------------------GCTACCCGGCGCCTGCCTTG`	`22 bp deleted → frameshift`
127	`CCACTGCCATCTCCGCGG-------------------------------CGCCTGCCTTG`	`31 bp deleted → frameshift`
126	`CCACTGCCATCTCCGCGGTCGCC-----------------------CGGCGCCTGCCTTG`	`23 bp deleted → frameshift`
126	`CCACTGCCATCTCCGCGG-----------------------CTACCCGGCGCCTGCCTTG`	`23 bp deleted → frameshift`
120	`CCACTGCCATCTCCGCGGTCGCCGTCG------------------------CCTGCCTTG`	`24 bp deleted → no frameshift`
115	`CCACTGCCATCTCCG---------------------------------GCGCCTGCCTTG`	`33 bp deleted → no frameshift`
109	`CCACTGCCATCTCCGCGGTCGCC----------------------------------TTG`	`34 bp deleted → frameshift`
109	`CCACTGCCATCTCCGCG----------------------------------CCTGCCTTG`	`34 bp deleted → frameshift`
109	`CCACTGCCATCTCCGC--------------------------TACCCGGCGCCTGCCTTG`	`26 bp deleted → frameshift`
103	`CCACTGCCATCTCCGCGGTCGCCG---------------------------CCTGCCTTG`	`27 bp deleted → no frameshift`
103	`CCACTGCCATCTCCGCGGTCG---------------------------GCGCCTGCCTTG`	`27 bp deleted → no frameshift`
103	`CCACTGC---------------------------GCTGGGGCTACCCGGCGCCTGCCTTG`	`27 bp deleted → no frameshift`
101	`CCACTG--------------------------------GGGCTACCCGGCGCCTGCCTTG`	`32 bp deleted → frameshift`
99	`CCACTGCCATCTCCGC------------------------------------CTGCCTTG`	`36 bp deleted → no frameshift`
98	`CCACTGCCATCTCCGCGGTCGC----------------------------GCCTGCCTTG`	`28 bp deleted → frameshift`
94	`CCACTGC-----------------------------TGGGGCTACCCGGCGCCTGCCTTG`	`29 bp deleted → frameshift`
86	`CCACTGCCATCT-------------------------GGGGCTACCCGGCGCCTGCCTTG`	`25 bp deleted → frameshift`
80	`CCACTGCCATCTCC--------------------------------CGGCGCCTGCCTTG`	`32 bp deleted → frameshift`
77	`CCACTGCC-------------------------------------------------TTG`	`49 bp deleted → frameshift`
69	`CCACTGC-----------------------------------TACCCGGCGCCTGCCTTG`	`35 bp deleted → frameshift`
63	`CCACTGCCATCT-------------------------------ACCCGGCGCCTGCCTTG`	`31 bp deleted → frameshift`
63	`CCACTGCC---------------------------------------------TGCCTTG`	`45 bp deleted → no frameshift`
60	`CCACTGCC--------------------------------------CGGCGCCTGCCTTG`	`38 bp deleted → frameshift`
56	`CCACTGCCATCTCC---------------------------------------TGCCTTG`	`39 bp deleted → no frameshift`
56	`CCACTGCC---------------------------------------GGCGCCTGCCTTG`	`39 bp deleted → no frameshift`
54	`CCACTGCCATCTCCGC----------------------------------------CTTG`	`40 bp deleted → frameshift`
46	`CCACTGCCATCTCC-------------------------------------------TTG`	`43 bp deleted → frameshift`
46	`CCACTGC-------------------------------------------GCCTGCCTTG`	`43 bp deleted → frameshift`
45	`CCACT--------------------------------------ACCCGGCGCCTGCCTTG`	`38 bp deleted → frameshift`
44	`CC--------------------------------------------CGGCGCCTGCCTTG`	`44 bp deleted → frameshift`
42	`CC---------------------------------------------GGCGCCTGCCTTG`	`45 bp deleted → no frameshift`
38	`CCAC-----------------------------------------CCGGCGCCTGCCTTG`	`41 bp deleted → frameshift`
36	`CCACTGCCATCT------------------------------------------GCCTTG`	`42 bp deleted → no frameshift`
31	`CC---------------------------------------------------TGCCTTG`	`51 bp deleted → no frameshift`
30	`CCACTGCCATCT----------------------------------------------TG`	`46 bp deleted → frameshift`
25	`CC-------------------------------------------------------TTG`	`55 bp deleted → frameshift`
21	`CCACT-----------------------------------------------------TG`	`53 bp deleted → frameshift`
20	`CCACTG------------------------------------------------------`	`54 bp deleted → no frameshift`

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