CRISPOR

DNA break repair outcome predictions

Guide: GCGTTCCCGGTGGCGCAGTC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTTTGCTGTGCTGGTACTCGGCGTTCCCGGTGGCGCAGTCTGGGGTGCAGCTCACCTCGATTTCTTCATAGAAATCCGAT`	(Wild-type sequence)
384	`CTGGTACTCGGCGTTCCCGGTGGCGCAG------------CTCACCTCGATTTCTTCATA`	`12 bp deleted → no frameshift`
330	`CTGGTACTCGGCGTTCCCGGTG---------------CAGCTCACCTCGATTTCTTCATA`	`15 bp deleted → no frameshift`
303	`CTGGTACTCGGCGTTCCCGGTGG----------GGTGCAGCTCACCTCGATTTCTTCATA`	`10 bp deleted → frameshift`
230	`CTGGTACTCGGCGTTCCCGGTGG-----------GTGCAGCTCACCTCGATTTCTTCATA`	`11 bp deleted → frameshift`
219	`CTGGTACTCGGCGTTCCCGGTGG------------TGCAGCTCACCTCGATTTCTTCATA`	`12 bp deleted → no frameshift`
211	`CTGGTACTCGGCGTTCCCGGTGGCGCAGT-------GCAGCTCACCTCGATTTCTTCATA`	`7 bp deleted → frameshift`
208	`CTGGTACTCGGCGTTCCCGG-------------GGTGCAGCTCACCTCGATTTCTTCATA`	`13 bp deleted → frameshift`
198	`CTGGTACTCGGCGTTCCCGGTGGC--------------AGCTCACCTCGATTTCTTCATA`	`14 bp deleted → frameshift`
198	`CTGGTACTCGGCGTTCCCGG--------------GTGCAGCTCACCTCGATTTCTTCATA`	`14 bp deleted → frameshift`
188	`CTGGTACTCGGCGTTCCCGGTGGCGC---------------TCACCTCGATTTCTTCATA`	`15 bp deleted → no frameshift`
170	`CTGGTACTCGGCGTTCCCGGTGGC-----------------TCACCTCGATTTCTTCATA`	`17 bp deleted → frameshift`
156	`CTGGTACTCGGCGTTCCCGGTGGCGCAGTC-------------ACCTCGATTTCTTCATA`	`13 bp deleted → frameshift`
133	`CTGGTACTCGG----------------------GGTGCAGCTCACCTCGATTTCTTCATA`	`22 bp deleted → frameshift`
128	`CTGGTACTCGGCGTTCCCGGTGGCGCA-----------------CCTCGATTTCTTCATA`	`17 bp deleted → frameshift`
126	`CTGGTACTCGG-----------------------GTGCAGCTCACCTCGATTTCTTCATA`	`23 bp deleted → frameshift`
122	`CTGGTACTCGGCGTTCCCGGTGGCGCAGTC------------------GATTTCTTCATA`	`18 bp deleted → no frameshift`
120	`CTGGTACTCGGCGTTCCCGGTGGCG------------------------ATTTCTTCATA`	`24 bp deleted → no frameshift`
120	`CTGGTACTCGG------------------------TGCAGCTCACCTCGATTTCTTCATA`	`24 bp deleted → no frameshift`
117	`CTGG-----------------------------GGTGCAGCTCACCTCGATTTCTTCATA`	`29 bp deleted → frameshift`
109	`CTGGTACTCGGC--------------------------AGCTCACCTCGATTTCTTCATA`	`26 bp deleted → frameshift`
106	`CTGGT-------------------------------GCAGCTCACCTCGATTTCTTCATA`	`31 bp deleted → frameshift`
99	`CTGGTACTCGGCGT----------------------GCAGCTCACCTCGATTTCTTCATA`	`22 bp deleted → frameshift`
99	`CTGGTACTCG---------------------------------------ATTTCTTCATA`	`39 bp deleted → no frameshift`
98	`CTGGTACTCGGCGTTCCC----------------------------TCGATTTCTTCATA`	`28 bp deleted → frameshift`
94	`CTGGTACTCGGCGTTCC-----------------------------TCGATTTCTTCATA`	`29 bp deleted → frameshift`
94	`CTGGTACTCGGC-----------------------------TCACCTCGATTTCTTCATA`	`29 bp deleted → frameshift`
91	`CTGGTACTC----------------------------------ACCTCGATTTCTTCATA`	`34 bp deleted → frameshift`
90	`CTGGTACTCGGCGTTCCCGGTGGCGCAGTC------------------------TTCATA`	`24 bp deleted → no frameshift`
89	`CTGGTACTCGGCGTTCCCG------------------------------ATTTCTTCATA`	`30 bp deleted → no frameshift`
89	`CTGG------------------------------GTGCAGCTCACCTCGATTTCTTCATA`	`30 bp deleted → no frameshift`
77	`CTGGTACTCGGCGTTCCCGGTGGCGCAGTC---------------------------ATA`	`27 bp deleted → no frameshift`
77	`CTGGTACTCGGCGTTC---------------------------ACCTCGATTTCTTCATA`	`27 bp deleted → no frameshift`
66	`CTGGTACTCGGCG------------------------------------ATTTCTTCATA`	`36 bp deleted → no frameshift`
63	`CTGGTACTCGGCGTTCCCGGTGGCGCA-------------------------------TA`	`31 bp deleted → frameshift`
60	`CTGGTACTCGGCGTTC--------------------------------GATTTCTTCATA`	`32 bp deleted → frameshift`
60	`CTGGTACTCGGCGTTC--------------------------------------TTCATA`	`38 bp deleted → frameshift`
54	`CTG----------------------------------CAGCTCACCTCGATTTCTTCATA`	`34 bp deleted → frameshift`
51	`CTGGTACTCGGCGTTC-----------------------------------------ATA`	`41 bp deleted → frameshift`
45	`CTGGTAC--------------------------------------CTCGATTTCTTCATA`	`38 bp deleted → frameshift`
40	`CT----------------------------------------CACCTCGATTTCTTCATA`	`40 bp deleted → frameshift`
31	`CTGGTACTC---------------------------------------------TTCATA`	`45 bp deleted → no frameshift`
31	`CT---------------------------------------------CGATTTCTTCATA`	`45 bp deleted → no frameshift`
31	`CTGGTACTCGGCGTT-------------------------------------TCTTCATA`	`37 bp deleted → frameshift`
28	`CTGGTACT-----------------------------------------------TCATA`	`47 bp deleted → frameshift`
27	`CTGGTACTC------------------------------------------------ATA`	`48 bp deleted → no frameshift`
21	`CT-----------------------------------------------------TCATA`	`53 bp deleted → frameshift`
13	`CTGGTA------------------------------------------------------`	`54 bp deleted → no frameshift`

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