CRISPOR

DNA break repair outcome predictions

Guide: CGCCACTCGGCGAACGCAAG TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CATTACAGTACCGCTGGTGACGCCACTCGGCGAACGCAAGTGGATAAATAGAAACGTCTGCCACAGTGAAGATGAAAGGA`	(Wild-type sequence)
220	`CCGCTGGTGACGCCACTCGGCGAACG--------------------TCTGCCACAGTGAA`	`20 bp deleted → frameshift`
208	`CCGCTGGTGACGCCACTCGG-------------ATAAATAGAAACGTCTGCCACAGTGAA`	`13 bp deleted → frameshift`
173	`CCGCTGGTGACGCCACTCGGCGAACGCAAG-----------AAACGTCTGCCACAGTGAA`	`11 bp deleted → frameshift`
173	`CCGCTGGTGACGCCACTCGGCGA-----------TAAATAGAAACGTCTGCCACAGTGAA`	`11 bp deleted → frameshift`
154	`CCGCTGGTGACGCCACTCGGCGAA-------------------ACGTCTGCCACAGTGAA`	`19 bp deleted → frameshift`
136	`CCGCTGG--------------------------ATAAATAGAAACGTCTGCCACAGTGAA`	`26 bp deleted → frameshift`
135	`CCGCTGGTGACGCCAC--------------------------------------AGTGAA`	`38 bp deleted → frameshift`
134	`CCGCTGGTGACGCCACTCGGCGAACGCAA--------ATAGAAACGTCTGCCACAGTGAA`	`8 bp deleted → frameshift`
127	`CCGCTGGTGACGCCACTCGGCGAACGCAA---------TAGAAACGTCTGCCACAGTGAA`	`9 bp deleted → no frameshift`
120	`CCGCTGGTGACGCCACTCGGCGAACGC------------------------CACAGTGAA`	`24 bp deleted → no frameshift`
120	`CCGCTGGTGACGCCACTCGGCG------------------------TCTGCCACAGTGAA`	`24 bp deleted → no frameshift`
104	`CCGCTGGTGACGCCACTCGGCGAA-------------ATAGAAACGTCTGCCACAGTGAA`	`13 bp deleted → frameshift`
103	`CCGCTGGTGACGCCACTCG---------------------------TCTGCCACAGTGAA`	`27 bp deleted → no frameshift`
99	`CCGCTGGTGACGCCACTCGGCGAACGCAA--------------ACGTCTGCCACAGTGAA`	`14 bp deleted → frameshift`
99	`CCGCTGGTGACGCCACTCGGCGAA--------------TAGAAACGTCTGCCACAGTGAA`	`14 bp deleted → frameshift`
95	`CCGCTGGTG-----------------------GATAAATAGAAACGTCTGCCACAGTGAA`	`23 bp deleted → frameshift`
94	`CCGCTGGTGACGCCACTCGGCGAACGCAA---------------CGTCTGCCACAGTGAA`	`15 bp deleted → no frameshift`
91	`CCGCTGGTGACG----------------------------------TCTGCCACAGTGAA`	`34 bp deleted → frameshift`
90	`CCGCTGGTGA------------------------TAAATAGAAACGTCTGCCACAGTGAA`	`24 bp deleted → no frameshift`
89	`CCGCTGGTGACGCCACTCGGC------------------------------CACAGTGAA`	`30 bp deleted → no frameshift`
86	`CCGCTGGTGACGCCACTCGGCGAACGCA-------------------------CAGTGAA`	`25 bp deleted → frameshift`
81	`CCGCTGGTGACGCCACTCGGCGAACGCAAG--------------------------TGAA`	`26 bp deleted → frameshift`
77	`CCGCTGGTGACGCCACTCGGCGAACGCA---------------------------GTGAA`	`27 bp deleted → no frameshift`
70	`CCGCTGGTGACGCCACTCGGCGAAC-----------------------------AGTGAA`	`29 bp deleted → frameshift`
70	`CCGCTGGTGACGCCAC-----------------------------GTCTGCCACAGTGAA`	`29 bp deleted → frameshift`
66	`CCGCTGGTGACGCCACTC------------------------------TGCCACAGTGAA`	`30 bp deleted → no frameshift`
66	`CCGCTGGTGACGCCACTCGGCGAA------------------------------------`	`36 bp deleted → no frameshift`
60	`CCGCTGGTGACGCCACT--------------------------------GCCACAGTGAA`	`32 bp deleted → frameshift`
60	`CCGCTGGTGA--------------------------------AACGTCTGCCACAGTGAA`	`32 bp deleted → frameshift`
55	`CCGCTG--------------------------------------------CCACAGTGAA`	`44 bp deleted → frameshift`
51	`CCGCTGGTGA-------------------------------------------------A`	`49 bp deleted → frameshift`
46	`CCG-------------------------------------------TCTGCCACAGTGAA`	`43 bp deleted → frameshift`
42	`CCGCTGGT---------------------------------------CTGCCACAGTGAA`	`39 bp deleted → no frameshift`
42	`CCGCTGGTGACGCCACTCGGCGAACGCAA-------------------------------`	`31 bp deleted → frameshift`
40	`CCGCTGGTGACGCCA----------------------------------------GTGAA`	`40 bp deleted → frameshift`
38	`CCGCTGGTG-----------------------------------------CCACAGTGAA`	`41 bp deleted → frameshift`
38	`CCGC-----------------------------------------------CACAGTGAA`	`47 bp deleted → frameshift`
34	`CCGCTGGTGAC-------------------------------------------AGTGAA`	`43 bp deleted → frameshift`
32	`CC--------------------------------------------------ACAGTGAA`	`50 bp deleted → frameshift`
22	`CCGCTG----------------------------------------------------AA`	`52 bp deleted → frameshift`

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