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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313787_F | TCGTCGGCAGCGTCCCTTGTCGCCTTAGCTCCTC | 60.1 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313787_R | GTCTCGTGGGCTCGGGTCCTTTCGGCTCTCCATCC | 60.1 | 1.00 |
| mm4_intron_Grm7_chr6_111103688_F | Primer3: not found at this Tm | N.d. | 0.47 |
| mm4_intron_Grm7_chr6_111103688_R | Primer3: not found at this Tm | N.d. | 0.47 |
| mm3_intron_Ndc1_chr4_107411335_F | TCGTCGGCAGCGTCGCCTGCCCTTAGGAGTTCTC | 59.8 | 0.40 |
| mm3_intron_Ndc1_chr4_107411335_R | GTCTCGTGGGCTCGGTCCCCAGAGATGGTCATACC | 57.8 | 0.40 |
| mm4_intergenic_Palm2/Gm20459|Gm20459/Palm2_chr4_57626638_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Palm2/Gm20459|Gm20459/Palm2_chr4_57626638_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Eepd1|Gm26190_chr9_25811487_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Eepd1|Gm26190_chr9_25811487_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm27018|Gm8652_chr6_6341123_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm27018|Gm8652_chr6_6341123_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Cisd2_chr3_135419491_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Cisd2_chr3_135419491_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Psma4|Chrna5_chr9_54972868_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Psma4|Chrna5_chr9_54972868_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Gm26134|Gm23434_chr1_98564686_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm26134|Gm23434_chr1_98564686_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Itgae_chr11_73131402_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Itgae_chr11_73131402_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Htr1a|Dph3b-ps_chr13_106277609_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Htr1a|Dph3b-ps_chr13_106277609_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm1968|4632428C04Rik_chr16_30000735_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm1968|4632428C04Rik_chr16_30000735_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Gpc5_chr14_115302955_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Gpc5_chr14_115302955_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm3_intergenic_Sfxn5|Rab11fip5/Mir705_chr6_85334541_F | TCGTCGGCAGCGTCCCTTGACTCTAGGGGAGCCT | 60.0 | 0.16 |
| mm3_intergenic_Sfxn5|Rab11fip5/Mir705_chr6_85334541_R | GTCTCGTGGGCTCGGAATGGCTCATGCTGTGTCCA | 59.9 | 0.16 |
| mm4_intron_Mob3b_chr4_35057565_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Mob3b_chr4_35057565_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Spata13_chr14_60675235_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Spata13_chr14_60675235_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Rasgef1c_chr11_49967638_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Rasgef1c_chr11_49967638_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Igsf3|Atp1a1_chr3_101560702_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Igsf3|Atp1a1_chr3_101560702_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Mfsd2a_chr4_122954757_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Mfsd2a_chr4_122954757_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm25509|n-R5-8s1_chr18_73104639_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm25509|n-R5-8s1_chr18_73104639_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_D630008O14Rik|Bcl2_chr1_106625465_F | TCGTCGGCAGCGTCAGCACAGTAGAGGAGGAGGT | 59.2 | 0.13 |
| mm4_intergenic_D630008O14Rik|Bcl2_chr1_106625465_R | GTCTCGTGGGCTCGGTCTAAATGTTTTAAAGTTCGCTGGT | 57.7 | 0.13 |
| mm4_intergenic_Sept5|Cldn5_chr16_18664790_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Sept5|Cldn5_chr16_18664790_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Zfp644_chr5_106645876_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Zfp644_chr5_106645876_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm22438|Gm22159_chr1_108142445_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm22438|Gm22159_chr1_108142445_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Tll1|Mir710_chr8_64364162_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Tll1|Mir710_chr8_64364162_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intergenic_Higd1a|Ackr2_chr9_121868442_F | TCGTCGGCAGCGTCCACTGACGTACTTAACAGTGAGGA | 60.0 | 0.08 |
| mm3_intergenic_Higd1a|Ackr2_chr9_121868442_R | GTCTCGTGGGCTCGGGAACCTCGGTCATGCTGGAA | 60.0 | 0.08 |
| mm4_intron_Acot7_chr4_152267595_F | TCGTCGGCAGCGTCACAGGGTCCCTTTGCTTCAC | 60.1 | 0.08 |
| mm4_intron_Acot7_chr4_152267595_R | GTCTCGTGGGCTCGGGCTGGAAGAAAGGAGCTGGT | 59.9 | 0.08 |
| mm4_intron_Ubash3b_chr9_41158425_F | TCGTCGGCAGCGTCAAGGGTTCTGAGTCGGGAGT | 60.1 | 0.08 |
| mm4_intron_Ubash3b_chr9_41158425_R | GTCTCGTGGGCTCGGGTGGAGGTTGCTGGTCCTAC | 60.0 | 0.08 |
| mm4_intergenic_Cadm2|Gm24681_chr16_68105912_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Cadm2|Gm24681_chr16_68105912_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Abcc12_chr8_86559528_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Abcc12_chr8_86559528_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Pld1_chr3_27965807_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Pld1_chr3_27965807_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mical2|Micalcl_chr7_112356585_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Mical2|Micalcl_chr7_112356585_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Gm22159|Gm25293_chr1_109765794_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Gm22159|Gm25293_chr1_109765794_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Nbea_chr3_55969690_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Nbea_chr3_55969690_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Cdh5|Bean1_chr8_104160809_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Cdh5|Bean1_chr8_104160809_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Top1mt_chr15_75670579_F | TCGTCGGCAGCGTCTTGTGGCCCTAACTCACACC | 59.8 | 0.05 |
| mm4_intron_Top1mt_chr15_75670579_R | GTCTCGTGGGCTCGGTGGACTCTTTGGGTTTGGGG | 59.8 | 0.05 |
| mm4_intergenic_Slit3|Mir218-2_chr11_35614601_F | TCGTCGGCAGCGTCTTTCCCCTAAAAGCAGCCCC | 60.2 | 0.05 |
| mm4_intergenic_Slit3|Mir218-2_chr11_35614601_R | GTCTCGTGGGCTCGGGCTGGTAAAGAGGCAGAGCA | 60.0 | 0.05 |
| mm4_intergenic_Gm25974|Epha4_chr1_77328114_F | TCGTCGGCAGCGTCAGGCCTAGATAGCAGCACCT | 60.1 | 0.05 |
| mm4_intergenic_Gm25974|Epha4_chr1_77328114_R | GTCTCGTGGGCTCGGCGATCCAATTGACCCCGCTA | 59.8 | 0.05 |
| mm4_intron_Yipf1_chr4_107315487_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Yipf1_chr4_107315487_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Stim2|Gm24235_chr5_54288474_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Stim2|Gm24235_chr5_54288474_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Pkhd1_chr1_20389518_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Pkhd1_chr1_20389518_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Setbp1|Gm25824_chr18_79133067_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Setbp1|Gm25824_chr18_79133067_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Vegfc|Spcs3_chr8_54373316_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Vegfc|Spcs3_chr8_54373316_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm11510|Msi2_chr11_88460762_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm11510|Msi2_chr11_88460762_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Npvf|G930045G22Rik_chr6_50835095_F | TCGTCGGCAGCGTCTAGCATCACCCAAGTGGCTC | 59.7 | 0.04 |
| mm4_intergenic_Npvf|G930045G22Rik_chr6_50835095_R | GTCTCGTGGGCTCGGGAGCTTCTCAGAGACGAGCC | 59.8 | 0.04 |
| mm4_intergenic_Gm22967|Dlgap1_chr17_69617584_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm22967|Dlgap1_chr17_69617584_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm24246|Pik3c3_chr18_30151808_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm24246|Pik3c3_chr18_30151808_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm9915|2610017I09Rik_chr1_42410644_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Gm9915|2610017I09Rik_chr1_42410644_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Olfr225|Mprip_chr11_59641514_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Olfr225|Mprip_chr11_59641514_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm23345|Usp47_chr7_111950231_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm23345|Usp47_chr7_111950231_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ppp3ca_chr3_136898346_F | TCGTCGGCAGCGTCGTGTCTCTGCATCCCTCTGG | 59.8 | 0.03 |
| mm4_intron_Ppp3ca_chr3_136898346_R | GTCTCGTGGGCTCGGCTGTCTACAACGCTGGAGCT | 59.7 | 0.03 |
| mm4_intergenic_Gm24880|Gm10172_chr7_71017670_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm24880|Gm10172_chr7_71017670_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122732333_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm10612/Cbfa2t3|Acsf3_chr8_122732333_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15228|Tmsb4x_chrX_167188513_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm15228|Tmsb4x_chrX_167188513_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Klhdc8a_chr1_132306013_F | TCGTCGGCAGCGTCGCTCTTTGTGCTACTTCCCAG | 58.9 | 0.02 |
| mm4_exon_Klhdc8a_chr1_132306013_R | GTCTCGTGGGCTCGGCCTCAGGGCTAACTCAGAGC | 59.5 | 0.02 |
| mm4_intron_Dock4_chr12_40631374_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dock4_chr12_40631374_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ly6f|9030619P08Rik_chr15_75377051_F | TCGTCGGCAGCGTCCCTCAGCACAAGAGGCTTCA | 59.9 | 0.02 |
| mm4_intergenic_Ly6f|9030619P08Rik_chr15_75377051_R | GTCTCGTGGGCTCGGCAGTACCCGAGATGTGCTGG | 60.1 | 0.02 |
| mm4_intergenic_Mc2r|mmu-mir-6356_chr18_68445585_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mc2r|mmu-mir-6356_chr18_68445585_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm25166|Pi15_chr1_17543781_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm25166|Pi15_chr1_17543781_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_AC166813.1|2610316D01Rik_chr3_45257208_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_AC166813.1|2610316D01Rik_chr3_45257208_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ggta1_chr2_35408635_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ggta1_chr2_35408635_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Gm5815|2610034M16Rik_chr17_58620135_F | TCGTCGGCAGCGTCAGCAAAGTCAGAGACAGACTC | 57.3 | 0.01 |
| mm3_intergenic_Gm5815|2610034M16Rik_chr17_58620135_R | GTCTCGTGGGCTCGGAAGTGCAGGATGGCCTTCTC | 60.0 | 0.01 |
| mm3_intergenic_Cbln2|Gm5096_chr18_87070841_F | TCGTCGGCAGCGTCTGAGGTAGAGAAGAGGGGAGA | 58.4 | 0.01 |
| mm3_intergenic_Cbln2|Gm5096_chr18_87070841_R | GTCTCGTGGGCTCGGTCTGTTCTAGCACATGAACAGGT | 59.6 | 0.01 |
| mm4_exon_Gm21975/Evi2b_chr11_79514548_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Gm21975/Evi2b_chr11_79514548_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Pcmtd1_chr1_7152270_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Pcmtd1_chr1_7152270_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tnrc6a|Slc5a11_chr7_123197571_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tnrc6a|Slc5a11_chr7_123197571_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Slc30a10|Lyplal1_chr1_185742049_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Slc30a10|Lyplal1_chr1_185742049_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Chrdl1_chrX_143303089_F | TCGTCGGCAGCGTCTGAATGTTGGGTGTCCTGCT | 59.5 | 0.00 |
| mm4_intron_Chrdl1_chrX_143303089_R | GTCTCGTGGGCTCGGGAGGCAGCCTCTAACACTGT | 59.3 | 0.00 |
| mm4_intergenic_Gm25704|Gm14103_chr2_133045945_F | TCGTCGGCAGCGTCAACTGAGCAAGAGGCCCAAA | 59.8 | 0.00 |
| mm4_intergenic_Gm25704|Gm14103_chr2_133045945_R | GTCTCGTGGGCTCGGCCAGTCCCAACATCTTCGCT | 60.0 | 0.00 |
| mm4_intergenic_Slitrk5|Gm22764_chr14_111814473_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slitrk5|Gm22764_chr14_111814473_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15219|Cdkn3_chr14_46757958_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15219|Cdkn3_chr14_46757958_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hs1bp3|Rhob_chr12_8448692_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Hs1bp3|Rhob_chr12_8448692_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm4913|Gm16420_chrX_120047381_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm4913|Gm16420_chrX_120047381_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Auts2_chr5_131707747_F | TCGTCGGCAGCGTCGTTTTGGCAAGCGGTCTGG | 60.0 | 0.00 |
| mm3_intron_Auts2_chr5_131707747_R | GTCTCGTGGGCTCGGTCTGGAGCCTGTTCAAACTCA | 59.2 | 0.00 |
| mm4_intergenic_Nek7|Lhx9_chr1_138771302_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Nek7|Lhx9_chr1_138771302_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ccser1|Gm22212_chr6_62872945_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ccser1|Gm22212_chr6_62872945_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ttc37_chr13_76167459_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ttc37_chr13_76167459_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rfesd_chr13_76006858_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rfesd_chr13_76006858_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fbxl4_chr4_22385450_F | TCGTCGGCAGCGTCCTTCATGCAAGTGCTCCTGC | 59.8 | 0.00 |
| mm4_intron_Fbxl4_chr4_22385450_R | GTCTCGTGGGCTCGGCCACAGGTATCTCCTCTTCAGC | 59.8 | 0.00 |
| mm4_intron_Dync1i1_chr6_5893608_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dync1i1_chr6_5893608_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ccbl2_chr3_142721653_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ccbl2_chr3_142721653_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Vmn2r90|Gm23837_chr17_17748307_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Vmn2r90|Gm23837_chr17_17748307_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ttc27|Ltbp1_chr17_74957252_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ttc27|Ltbp1_chr17_74957252_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slc9a1|Gm13257_chr4_133444281_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Slc9a1|Gm13257_chr4_133444281_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sik2|Layn_chr9_51022988_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Sik2|Layn_chr9_51022988_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Olfr446|Olfr444_chr6_42952345_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Olfr446|Olfr444_chr6_42952345_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Olfr294|Olfr293_chr7_86656933_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Olfr294|Olfr293_chr7_86656933_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Msr1|Fgf20_chr8_40135802_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Msr1|Fgf20_chr8_40135802_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lpl|Slc18a1_chr8_69014010_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Lpl|Slc18a1_chr8_69014010_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm9578|Gm22891_chr14_81961915_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm9578|Gm22891_chr14_81961915_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25656|Cntn6_chr6_103882696_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25656|Cntn6_chr6_103882696_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23203|Gm23447_chr17_59823999_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23203|Gm23447_chr17_59823999_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fryl|Ociad1_chr5_73292504_F | TCGTCGGCAGCGTCAGGGAAGTCCAAGGAAAGTCA | 58.5 | 0.00 |
| mm4_intergenic_Fryl|Ociad1_chr5_73292504_R | GTCTCGTGGGCTCGGAGCTCTGCCTCTAGGTTCGA | 60.0 | 0.00 |
| mm4_intergenic_AC161214.1|Gm11033_chr8_74367535_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_AC161214.1|Gm11033_chr8_74367535_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Zfp641_chr15_98289062_F | TCGTCGGCAGCGTCAGGGCACGTGTGTGGTTTTA | 60.1 | 0.00 |
| mm4_exon_Zfp641_chr15_98289062_R | GTCTCGTGGGCTCGGGTCCAAGCTGGGAGTCCATG | 60.3 | 0.00 |
| mm4_exon_2310061I04Rik/Gm16279_chr17_35895807_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_2310061I04Rik/Gm16279_chr17_35895807_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313787 |
CCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTC CCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGAC |
| mm3_intron_Ndc1_chr4_107411335 |
GCCTGCCCTTAGGAGTTCTCCAAGAGCTAATACGAGAAGGCTTGTGCATTGAAAGTTTTTTCTAGGTCCTTGATGGGCCT CTGGGATTAAGAGCTTACATATGGACTTGGTATGACCATCTCTGGGGA |
| mm3_intergenic_Sfxn5|Rab11fip5/Mir705_chr6_85334541 |
CCTTGACTCTAGGGGAGCCTCTGTCTCATGGTGGGATTCAGAGCTAACTTGGGGTTGCCCTCTTGTCCTTCTGGACACAG CATGAGCCATT |
| mm4_intergenic_D630008O14Rik|Bcl2_chr1_106625465 |
AGCACAGTAGAGGAGGAGGTTGAACTTTCATCAGGTGGGACAGTTATCCCATGTAGAGAAAAAAATCTCTAACTCACTTT ATTCCCCCAAGTAAGATTCAAATCCTAATAAAACCAGCGAACTTTAAAACATTTAGA |
| mm3_intergenic_Higd1a|Ackr2_chr9_121868442 |
CACTGACGTACTTAACAGTGAGGAAAAACATGGGGTTTGGAGCTCACTTGGGGAAGCATTGGTGCTGTTCCAGCATGACC GAGGTTC |
| mm4_intron_Acot7_chr4_152267595 |
ACAGGGTCCCTTTGCTTCACATGGCATGAGGAACCTACTTGTGGCTGCAGGTGGGCAGTCTAGCAGCCAAGAGCTGCCCA TGGCCCTGTGGCCACCAGCTCCTTTCTTCCAGC |
| mm4_intron_Ubash3b_chr9_41158425 |
AAGGGTTCTGAGTCGGGAGTCGGGATCCCTGCTGCATAGGAGCCACAAGTGAGCTCCCACCCCCACCCCAGTAGGACCAG CAACCTCCAC |
| mm4_intron_Top1mt_chr15_75670579 |
TTGTGGCCCTAACTCACACCTTGAGCCTTACTCTGTCAGAAAATGACCAAATGAAATCCCCAAGTGAGCCCCTAAATCCA CATCCTTCCCCTAAAGCAAGGCCTCCCATGTCCCCAAACCCAAAGAGTCCA |
| mm4_intergenic_Slit3|Mir218-2_chr11_35614601 |
TTTCCCCTAAAAGCAGCCCCCTTGAAAAAACAGCTGATGAGATTAGGAGCCAATTTGAGGGCTGCTCCTGCAACATGTCC AGCCCCTCCCTGGGTAGGGCGTCTGGCTTCCCAGCCACTTTGCTCTGCCTCTTTACCAGC |
| mm4_intergenic_Gm25974|Epha4_chr1_77328114 |
AGGCCTAGATAGCAGCACCTTCCTCAAAGAACCTGTTGGCATTTGAAGCTCACTTGAGGATGATAGCGGGGTCAATTGGA TCG |
| mm4_intergenic_Npvf|G930045G22Rik_chr6_50835095 |
TAGCATCACCCAAGTGGCTCATTCCGCAATCATGCTCCTAATCTCAAGGCTCATCTGCTCTCGTCTAAAGCTACGGAAAT GCTTTCCCACCTCATTCTTGTATTCAGACATTCCTCAGACGGCTCGTCTCTGAGAAGCTC |
| mm4_intron_Ppp3ca_chr3_136898346 |
GTGTCTCTGCATCCCTCTGGGACAAAGCATAAGTGATGGGTGGGCTTAGGAACTCACTGGCGGGATGGAGAAGCTCCAGC GTTGTAGACAG |
| mm4_exon_Klhdc8a_chr1_132306013 |
GCTCTTTGTGCTACTTCCCAGTCATTCATATTGGATTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGAACTAGCTCTGAGTTAGCCCTGAGG |
| mm4_intergenic_Ly6f|9030619P08Rik_chr15_75377051 | CCTCAGCACAAGAGGCTTCACCCACCACAAGTCAATTCCCAATCCCATGACCCCAGCACATCTCGGGTACTG |
| mm3_intergenic_Gm5815|2610034M16Rik_chr17_58620135 |
AGCAAAGTCAGAGACAGACTCGCAGTCATTTAAAGGGAGTAGCAGCTTACTTGAGACGTTTAGAGGTTTGTCTCTGAAAA TGCCTGCCTAATGGCTAATAGAGCCTCCCATACGTCTGAGAAGGCCATCCTGCACTT |
| mm3_intergenic_Cbln2|Gm5096_chr18_87070841 |
TGAGGTAGAGAAGAGGGGAGAAGTAAATTGTACTAAGTCCACAACTAAGCTCCTAATACAAAGGTAGGAAATAAATATAA AAATTAATGAGGTTGCTGTCATTAATACAACATTAACCTGTTCATGTGCTAGAACAGA |
| mm4_intron_Chrdl1_chrX_143303089 |
TGAATGTTGGGTGTCCTGCTTATTAAAAAAAAAAAAGTGGATTTTTTTTCCACAAGTGACCTCCAACTCCCAGAGGCAAT GTTTACAGTGTTAGAGGCTGCCTC |
| mm4_intergenic_Gm25704|Gm14103_chr2_133045945 |
AACTGAGCAAGAGGCCCAAATGGTATCAGGGGCTGACTTGCGGGAACAGTGACTTCGCACCTTCTTAGCGAAGATGTTGG GACTGG |
| mm3_intron_Auts2_chr5_131707747 |
GTTTTGGCAAGCGGTCTGGTGTTCCCCTACCAAGCTCCTAATCCCAGCAGATCAGTTAAAATAAATGAAAGAGAGAATCA AAAGGCACTTAAAAAATTCAACTGTTCTCCAAATGAGTTTGAACAGGCTCCAGA |
| mm4_intron_Fbxl4_chr4_22385450 |
CTTCATGCAAGTGCTCCTGCACACGTCCTCCCCACACGGAAGGAACATGGGATTTTGAGCTTTCATGGGGCCAATAACGG AAATAAAAATGCTGAAGAGGAGATACCTGTGG |
| mm4_intergenic_Fryl|Ociad1_chr5_73292504 |
AGGGAAGTCCAAGGAAAGTCATGGGATAAGGAGGGGACTTGAGGATGGAGGGGTTCCAAAATGTCCCTACTGGCTTGTCA CTGAACAGTCTCTGGGGTATGTCACAAGAGCATCGAACCTAGAGGCAGAGCT |
| mm4_exon_Zfp641_chr15_98289062 |
AGGGCACGTGTGTGGTTTTAAGAGAGAGTCTGTGTCTGTTCTGTGTAGATGGCGAGAGAAGGTGTCCTCCTGAAGGAAGC GTGGGCTTAGGAGCATTCCTGTGGAGTTCCTGGGCATGGACTCCCAGCTTGGAC |