CRISPOR

DNA break repair outcome predictions

Guide: GCGTGGCGGGGGAGGCCAGG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGG`	(Wild-type sequence)
519	`CTTCCCGGGAGCGTGGCGGGGGAGG-----------AGCCGGTCGATGGACTAGCAGGCA`	`11 bp deleted → frameshift`
430	`CTTCCCGGGAGCGTGGCGGGGGAGGCCAGG---AGGAGCCGGTCGATGGACTAGCAGGCA`	`3 bp deleted → no frameshift`
370	`CTTCCCGGGAGCGTGGCGGGGGAGGCCAGG------AGCCGGTCGATGGACTAGCAGGCA`	`6 bp deleted → no frameshift`
347	`CTTCCCGGGAGCGTGGCGGGGGAG--------------CCGGTCGATGGACTAGCAGGCA`	`14 bp deleted → frameshift`
335	`CTTCCCGGGAGCGTGGCGGGGGAGG--------AGGAGCCGGTCGATGGACTAGCAGGCA`	`8 bp deleted → frameshift`
313	`CTTCCCGGGAGCGTGGCGGGGGAGGCC-------------GGTCGATGGACTAGCAGGCA`	`13 bp deleted → frameshift`
233	`CTTCCCGGGAGC---------------------------CGGTCGATGGACTAGCAGGCA`	`27 bp deleted → no frameshift`
219	`CTTCCCGGGAGCGTGGCGGGGGAGGCCAGG------------TCGATGGACTAGCAGGCA`	`12 bp deleted → no frameshift`
219	`CTTCCCGGGAGCGTGGCGGGG------------AGGAGCCGGTCGATGGACTAGCAGGCA`	`12 bp deleted → no frameshift`
210	`CTTCCCGGGAG------------------------GAGCCGGTCGATGGACTAGCAGGCA`	`24 bp deleted → no frameshift`
208	`CTTCCCGGGAGCGTGGCGGG-------------AGGAGCCGGTCGATGGACTAGCAGGCA`	`13 bp deleted → frameshift`
198	`CTTCCCGGGAGCGTGGCGG--------------AGGAGCCGGTCGATGGACTAGCAGGCA`	`14 bp deleted → frameshift`
191	`CTTCCCGGGAGCGTGGCGGGGGAGGCCAG---------CCGGTCGATGGACTAGCAGGCA`	`9 bp deleted → no frameshift`
190	`CTTCCCGGGAGCGTGGCGG-----------------------TCGATGGACTAGCAGGCA`	`23 bp deleted → frameshift`
188	`CTTCCCGGGAGCGTGGCGGGG---------------AGCCGGTCGATGGACTAGCAGGCA`	`15 bp deleted → no frameshift`
179	`CTTCCCGGGAGCGTGGCGGG----------------AGCCGGTCGATGGACTAGCAGGCA`	`16 bp deleted → frameshift`
172	`CTTCCCGGGAGCGTGGCGGGGGAGGCCAGG----------------------------CA`	`28 bp deleted → frameshift`
170	`CTTCCCGGGAGCGTGGCGGGGGAGG-----------------TCGATGGACTAGCAGGCA`	`17 bp deleted → frameshift`
170	`CTTCCCGGGAGCGTGGCGG-----------------AGCCGGTCGATGGACTAGCAGGCA`	`17 bp deleted → frameshift`
170	`CTTCCCGGGAGCGTGG-----------------AGGAGCCGGTCGATGGACTAGCAGGCA`	`17 bp deleted → frameshift`
154	`CTTCCCGGGAGCGTGGCGGGGGAGGCCAGG-------------------ACTAGCAGGCA`	`19 bp deleted → frameshift`
147	`CTTCCCGGGAGCGTGGCGGGGG--------------------TCGATGGACTAGCAGGCA`	`20 bp deleted → frameshift`
147	`CTTCCCGGGAGCGTGG--------------------AGCCGGTCGATGGACTAGCAGGCA`	`20 bp deleted → frameshift`
146	`CTTCCCGG----------------------------------TCGATGGACTAGCAGGCA`	`34 bp deleted → frameshift`
140	`CTTCCCGGGAGCGTGGCGGGG---------------------TCGATGGACTAGCAGGCA`	`21 bp deleted → no frameshift`
134	`CTTCCCGGGAGCGTGGCGGGGGAGGC---------------------------------A`	`33 bp deleted → no frameshift`
133	`CTTCCCGGGAGCGTGGCGGG----------------------TCGATGGACTAGCAGGCA`	`22 bp deleted → frameshift`
133	`CTTCCCGGGAGCGTGGC----------------------CGGTCGATGGACTAGCAGGCA`	`22 bp deleted → frameshift`
129	`CTTCCCGGGAGCGTGGCGGGGGA---------------------------CTAGCAGGCA`	`27 bp deleted → no frameshift`
120	`CTTCCCGGGAGCGTGGCGGGGGAGG------------------------ACTAGCAGGCA`	`24 bp deleted → no frameshift`
114	`CTTCCCGG-------------------------AGGAGCCGGTCGATGGACTAGCAGGCA`	`25 bp deleted → frameshift`
109	`CTTCCCGGGAGCGTGG--------------------------TCGATGGACTAGCAGGCA`	`26 bp deleted → frameshift`
105	`CTTCCCGGGAG---------------------GAGGAGCCGGTCGATGGACTAGCAGGCA`	`21 bp deleted → no frameshift`
103	`CTTCCCGGGAGCGTGGCG---------------------------ATGGACTAGCAGGCA`	`27 bp deleted → no frameshift`
98	`CTTCCCGGGAGCGTGGCGGGG----------------------------ACTAGCAGGCA`	`28 bp deleted → frameshift`
98	`CTTCCCGGGAGCG----------------------------GTCGATGGACTAGCAGGCA`	`28 bp deleted → frameshift`
98	`CTTCCCGG----------------------------AGCCGGTCGATGGACTAGCAGGCA`	`28 bp deleted → frameshift`
96	`CTTCCCGGGAGCGTGG---------------------------------ACTAGCAGGCA`	`33 bp deleted → no frameshift`
95	`CTTCCCGGGAGCGTGGCGGGGGA-----------------------TGGACTAGCAGGCA`	`23 bp deleted → frameshift`
94	`CTTCCCGGGAGCGTGGCGGGGGAGGC-----------------------------AGGCA`	`29 bp deleted → frameshift`
94	`CTTCCCGGGAGCGTGGCGGG-----------------------------ACTAGCAGGCA`	`29 bp deleted → frameshift`
89	`CTTCCCGGGAGCGTGGCGG------------------------------ACTAGCAGGCA`	`30 bp deleted → no frameshift`
86	`CTTCCCGGGAGCGTGGCGGGGGAGGCCAG-------------------------CAGGCA`	`25 bp deleted → frameshift`
80	`CTTCCCGGGAGCG--------------------------------ATGGACTAGCAGGCA`	`32 bp deleted → frameshift`
76	`CTTCCCGGG---------------------------------TCGATGGACTAGCAGGCA`	`33 bp deleted → no frameshift`
73	`CTTCCCGGGAGCGTGGC------------------------------------------A`	`42 bp deleted → no frameshift`
70	`CTTCCCGGGAGCGT-----------------------------CGATGGACTAGCAGGCA`	`29 bp deleted → frameshift`
69	`CTTCC-----------------------------------GGTCGATGGACTAGCAGGCA`	`35 bp deleted → frameshift`
67	`CTTCCCGGGA----------------------------------------CTAGCAGGCA`	`40 bp deleted → frameshift`
66	`CTTCCCGGGAGCGTGGCGGGGGAG------------------------------CAGGCA`	`30 bp deleted → no frameshift`
66	`CTTCCCGGGAGCGTGGCGGGGG------------------------------------CA`	`36 bp deleted → no frameshift`
62	`CTTCCCGGGAGCGTGGCGGGG-------------------------------------CA`	`37 bp deleted → frameshift`
60	`CTTCCCGGGAGCGTGGCGGGGGAGGCCA--------------------------------`	`32 bp deleted → frameshift`
60	`CTTCCCGGGAGCGTGGCGGG--------------------------------------CA`	`38 bp deleted → frameshift`
60	`CTTCCCGGGAGCGTGGC--------------------------------------AGGCA`	`38 bp deleted → frameshift`
60	`CTTCCCG--------------------------------------ATGGACTAGCAGGCA`	`38 bp deleted → frameshift`
58	`CTTCCCGGGAGC-------------------------------------------AGGCA`	`43 bp deleted → frameshift`
56	`CTTCCCGGGAGCGTGGCGG---------------------------------------CA`	`39 bp deleted → no frameshift`
51	`CTTCCCGG-----------------------------------------ACTAGCAGGCA`	`41 bp deleted → frameshift`
49	`CTTCCCGGGA------------------------------------TGGACTAGCAGGCA`	`36 bp deleted → no frameshift`
40	`CTTC----------------------------------------GATGGACTAGCAGGCA`	`40 bp deleted → frameshift`
38	`CTTCCCGGGAGC-----------------------------------------------A`	`47 bp deleted → frameshift`
34	`CTTCCCGGG-------------------------------------------------CA`	`49 bp deleted → frameshift`
32	`CTTCCCGG--------------------------------------------------CA`	`50 bp deleted → frameshift`
30	`CTTCCCGGGAG----------------------------------------------GCA`	`46 bp deleted → frameshift`
24	`CT--------------------------------------------------AGCAGGCA`	`50 bp deleted → frameshift`

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