CRISPOR

DNA break repair outcome predictions

Guide: GGACTAGCAGGCAGTGCTGC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATG`	(Wild-type sequence)
469	`GCCGGTCGATGGACTAGCAGGCAGTGCTG--------GGCGCCGAGCCACGGTCTGCTGG`	`8 bp deleted → frameshift`
313	`GCCGGTCGATGGACTAGCAGGC-------------TGGGCGCCGAGCCACGGTCTGCTGG`	`13 bp deleted → frameshift`
311	`GCCGGTCGATGGACTAGCAGGCAGTGCTGC-----TGGGCGCCGAGCCACGGTCTGCTGG`	`5 bp deleted → frameshift`
274	`GCCGGTCGATGGACTAGCAGG------------GCTGGGCGCCGAGCCACGGTCTGCTGG`	`12 bp deleted → no frameshift`
244	`GCCGGTCGATGGACTAGCAGGC------------------GCCGAGCCACGGTCTGCTGG`	`18 bp deleted → no frameshift`
242	`GCCGGTCGATGGACTAGCAGGCAGTGCTGC----------GCCGAGCCACGGTCTGCTGG`	`10 bp deleted → frameshift`
219	`GCCGGTCGATGGACTAGCAGGCAGTGCTGC------------CGAGCCACGGTCTGCTGG`	`12 bp deleted → no frameshift`
208	`GCCGGTCGATGGACTAGCAGGCAGTGC-------------GCCGAGCCACGGTCTGCTGG`	`13 bp deleted → frameshift`
201	`GCCGGTCGATGGACTAGCAGGCAG--------GGCTGGGCGCCGAGCCACGGTCTGCTGG`	`8 bp deleted → frameshift`
188	`GCCGGTCGATGGACTAGCAGGCAGTGC---------------CGAGCCACGGTCTGCTGG`	`15 bp deleted → no frameshift`
181	`GCCGGTCGATGGACTAGCAGGCAGTGCTGC---------------------------TGG`	`27 bp deleted → no frameshift`
178	`GCCGGTCGATGGACTAGCAGGCAGTGCTG------------------------------G`	`30 bp deleted → no frameshift`
173	`GCCGGTCGATGGACTAGCAGGCAGTG-----------GGCGCCGAGCCACGGTCTGCTGG`	`11 bp deleted → frameshift`
170	`GCCGGTCGATGGACTAGCAGGCAGTGCTGC-----------------CACGGTCTGCTGG`	`17 bp deleted → frameshift`
170	`GCCGGTCGATGGACTAGCAGG-----------------GCGCCGAGCCACGGTCTGCTGG`	`17 bp deleted → frameshift`
170	`GCCGGTCGATGGACTAGC-----------------TGGGCGCCGAGCCACGGTCTGCTGG`	`17 bp deleted → frameshift`
147	`GCCGGTCGATGGACTAGCAGGCAGTGC--------------------CACGGTCTGCTGG`	`20 bp deleted → frameshift`
147	`GCCGGTCGATGGACTAGCAGGC--------------------CGAGCCACGGTCTGCTGG`	`20 bp deleted → frameshift`
141	`GCCGGTCGATGGACTAG---------------GGCTGGGCGCCGAGCCACGGTCTGCTGG`	`15 bp deleted → no frameshift`
140	`GCCGGTCGATGG---------------------GCTGGGCGCCGAGCCACGGTCTGCTGG`	`21 bp deleted → no frameshift`
136	`GCCGGTCGATGG--------------------------GCGCCGAGCCACGGTCTGCTGG`	`26 bp deleted → frameshift`
133	`GCCGGTCGATGGACTAGC----------------------GCCGAGCCACGGTCTGCTGG`	`22 bp deleted → frameshift`
133	`GCCGGTCGATGG----------------------CTGGGCGCCGAGCCACGGTCTGCTGG`	`22 bp deleted → frameshift`
120	`GCCGGTCGATGGACTAGC------------------------CGAGCCACGGTCTGCTGG`	`24 bp deleted → no frameshift`
117	`GCCGGTCGATGGACTAGC-----------------------------CACGGTCTGCTGG`	`29 bp deleted → frameshift`
114	`GCCGGTCGATGGACTAGCAGGC-------------------------CACGGTCTGCTGG`	`25 bp deleted → frameshift`
108	`GCCG----------------------------------------AGCCACGGTCTGCTGG`	`40 bp deleted → frameshift`
105	`GCCGGTCGATGGACT---------------------GGGCGCCGAGCCACGGTCTGCTGG`	`21 bp deleted → no frameshift`
103	`GCCGGTCGATGG---------------------------CGCCGAGCCACGGTCTGCTGG`	`27 bp deleted → no frameshift`
99	`GCCGGTCGATGGACTAGCAGGCAG----------------------CCACGGTCTGCTGG`	`22 bp deleted → frameshift`
98	`GCCGG----------------------------GCTGGGCGCCGAGCCACGGTCTGCTGG`	`28 bp deleted → frameshift`
94	`GCCGG-----------------------------CTGGGCGCCGAGCCACGGTCTGCTGG`	`29 bp deleted → frameshift`
85	`GCCGGTC-----------------------------------------------TGCTGG`	`47 bp deleted → frameshift`
84	`GCCGGTCGATGGACTAGCAGG-------------------------------TCTGCTGG`	`31 bp deleted → frameshift`
82	`GCCGGTCGA------------------------------------GCCACGGTCTGCTGG`	`36 bp deleted → no frameshift`
81	`GCCGGTCGATGGACTAGCAGGCA--------------------------CGGTCTGCTGG`	`26 bp deleted → frameshift`
81	`GCCGGTCGATGGACTAGCAG--------------------------CCACGGTCTGCTGG`	`26 bp deleted → frameshift`
76	`GCCGGTCG---------------------------------CCGAGCCACGGTCTGCTGG`	`33 bp deleted → no frameshift`
76	`GCCGG---------------------------------GCGCCGAGCCACGGTCTGCTGG`	`33 bp deleted → no frameshift`
76	`GC---------------------------------TGGGCGCCGAGCCACGGTCTGCTGG`	`33 bp deleted → no frameshift`
74	`GCCGGTCGATGGACTAGCAGGCAGT----------------------------CTGCTGG`	`28 bp deleted → frameshift`
73	`GCCGG----------------------------------CGCCGAGCCACGGTCTGCTGG`	`34 bp deleted → frameshift`
69	`GCCGGTCGATGGACTAGCAGGC-----------------------------------TGG`	`35 bp deleted → frameshift`
66	`GCCGGTCGATGGACTAGCA------------------------------CGGTCTGCTGG`	`30 bp deleted → no frameshift`
63	`GCC---------------------------------------------ACGGTCTGCTGG`	`45 bp deleted → no frameshift`
62	`GCCG-------------------------------------CCGAGCCACGGTCTGCTGG`	`37 bp deleted → frameshift`
60	`GCCGGTCGATGGA--------------------------------GCCACGGTCTGCTGG`	`32 bp deleted → frameshift`
60	`GC--------------------------------------GCCGAGCCACGGTCTGCTGG`	`38 bp deleted → frameshift`
57	`GCCGGTCGATGGACTAGCAGGCAGTG---------------------------------G`	`33 bp deleted → no frameshift`
56	`GCCGGTCGATGGACTAGCAGG---------------------------------------`	`39 bp deleted → no frameshift`
56	`GCCGGTCGATGGACTAGC---------------------------------------TGG`	`39 bp deleted → no frameshift`
54	`GCCGGTCGATGG----------------------------------------TCTGCTGG`	`40 bp deleted → frameshift`
49	`GCCGGTCGATGGAC------------------------------------GGTCTGCTGG`	`36 bp deleted → no frameshift`
46	`GCCGGTCG-------------------------------------------GTCTGCTGG`	`43 bp deleted → frameshift`
45	`GCCGGTCGATGG------------------------------------------------`	`48 bp deleted → no frameshift`
40	`GCCGGTCGATGGACT----------------------------------------GCTGG`	`40 bp deleted → frameshift`
34	`GCCGGTCGATGGACT-------------------------------------------GG`	`43 bp deleted → frameshift`
31	`GCCGGTCGATG---------------------------------------------CTGG`	`45 bp deleted → no frameshift`
25	`GCCGG-------------------------------------------------------`	`55 bp deleted → frameshift`
25	`GC-------------------------------------------------------TGG`	`55 bp deleted → frameshift`

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