CRISPOR

DNA break repair outcome predictions

Guide: TCTGCTGGAGCGGCCATGCT TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCT`	(Wild-type sequence)
485	`CGAGCCACGGTCTGCTGGAGCGGCC----------CGGGACCCCCAGTCCCCTCCGCGGA`	`10 bp deleted → frameshift`
311	`CGAGCCACGGTCTGCTGGAGCGGCCATGC-----CCGGGACCCCCAGTCCCCTCCGCGGA`	`5 bp deleted → frameshift`
283	`CGAGCCACGGTCTGCTGGAGCGG---------------GACCCCCAGTCCCCTCCGCGGA`	`15 bp deleted → no frameshift`
245	`CGAGCCACGGTCTGCTGGAGCGGCCATG----GCCCGGGACCCCCAGTCCCCTCCGCGGA`	`4 bp deleted → frameshift`
235	`CGAGCCACGGTCTGCTGG---------------CCCGGGACCCCCAGTCCCCTCCGCGGA`	`15 bp deleted → no frameshift`
230	`CGAGCCACGGTCTGCTGGAGCGGCC-----------GGGACCCCCAGTCCCCTCCGCGGA`	`11 bp deleted → frameshift`
208	`CGAGCCACGGTCTGCTGGAGC-------------CCGGGACCCCCAGTCCCCTCCGCGGA`	`13 bp deleted → frameshift`
184	`CGAGCCACGGTCTGCTGGAGCGGCCA--------------------GTCCCCTCCGCGGA`	`20 bp deleted → frameshift`
179	`CGAGCCACGGTCTGCTGGAGCGG----------------ACCCCCAGTCCCCTCCGCGGA`	`16 bp deleted → frameshift`
175	`CGAGCCACGGTCTGCTGGA---------------------CCCCCAGTCCCCTCCGCGGA`	`21 bp deleted → no frameshift`
170	`CGAGCCACGGTCTGCTGGAGCGGCC-----------------CCCAGTCCCCTCCGCGGA`	`17 bp deleted → frameshift`
162	`CGAGCCACGGTCTGCTGGAGCGGCC------------------CCAGTCCCCTCCGCGGA`	`18 bp deleted → no frameshift`
154	`CGAGCCACGGTCTGCTGGAGCGGCC-------------------CAGTCCCCTCCGCGGA`	`19 bp deleted → frameshift`
154	`CGAGCCACGGTCTGC-------------------CCGGGACCCCCAGTCCCCTCCGCGGA`	`19 bp deleted → frameshift`
148	`CGAGCCACGG----------------------------GACCCCCAGTCCCCTCCGCGGA`	`28 bp deleted → frameshift`
147	`CGAGCCACGGTCTGCTGG--------------------GACCCCCAGTCCCCTCCGCGGA`	`20 bp deleted → frameshift`
141	`CGAGCC-----------------------------CGGGACCCCCAGTCCCCTCCGCGGA`	`29 bp deleted → frameshift`
132	`CGAGCCACGGTCTGCTGGAGCGG------------------------------------A`	`36 bp deleted → no frameshift`
126	`CGAGCCACGG-----------------------CCCGGGACCCCCAGTCCCCTCCGCGGA`	`23 bp deleted → frameshift`
122	`CGAGCCACGGTCTG------------------GCCCGGGACCCCCAGTCCCCTCCGCGGA`	`18 bp deleted → no frameshift`
114	`CGAGCCACGGTCTGCTGGAGCGGCC-------------------------CCTCCGCGGA`	`25 bp deleted → frameshift`
109	`CGAGCCACGGTCTGCTGGAGCGGCC--------------------------CTCCGCGGA`	`26 bp deleted → frameshift`
103	`CGAGCCACGGTCTGCTGGAGCGGCC---------------------------TCCGCGGA`	`27 bp deleted → no frameshift`
98	`CGAGCCACGGTCTGCTGGAGCGGCCATGC----------------------------GGA`	`28 bp deleted → frameshift`
95	`CGAGCCACGGTCTGCTGGAGCGGCCATGCT-----------------------CCGCGGA`	`23 bp deleted → frameshift`
94	`CGAGCCACGG-----------------------------ACCCCCAGTCCCCTCCGCGGA`	`29 bp deleted → frameshift`
89	`CGAGCCACGGTCTGCTGGAGCGGCC------------------------------GCGGA`	`30 bp deleted → no frameshift`
89	`CGAGCC------------------------------GGGACCCCCAGTCCCCTCCGCGGA`	`30 bp deleted → no frameshift`
78	`CGAGCCACGGTC-------------------------------------CCCTCCGCGGA`	`37 bp deleted → frameshift`
77	`CGAGCCACGGTCTGCTGGAG---------------------------TCCCCTCCGCGGA`	`27 bp deleted → no frameshift`
76	`CGAGCCACGGTCTGCTGGAGCGGC---------------------------------GGA`	`33 bp deleted → no frameshift`
73	`CGAGCCACGGTCTGCTGGAGCG----------------------------------CGGA`	`34 bp deleted → frameshift`
71	`CGAGCCA---------------------------------------GTCCCCTCCGCGGA`	`39 bp deleted → no frameshift`
69	`CG-----------------------------------GGACCCCCAGTCCCCTCCGCGGA`	`35 bp deleted → frameshift`
66	`CGAGCC------------------------------------CCCAGTCCCCTCCGCGGA`	`36 bp deleted → no frameshift`
64	`CGAGCCACGGTCTGCTGGA-----------------------------------------`	`41 bp deleted → frameshift`
62	`CGAGCC-------------------------------------CCAGTCCCCTCCGCGGA`	`37 bp deleted → frameshift`
60	`CGAGCC--------------------------------------CAGTCCCCTCCGCGGA`	`38 bp deleted → frameshift`
57	`CGAGCCAC---------------------------------CCCCAGTCCCCTCCGCGGA`	`33 bp deleted → no frameshift`
51	`CGAGCCACGG-------------------------------------------------A`	`49 bp deleted → frameshift`
48	`CGAGCCACGGTCTGC------------------------------------------GGA`	`42 bp deleted → no frameshift`
47	`CGAGCCACGGTCTGCT-------------------------------------CCGCGGA`	`37 bp deleted → frameshift`
47	`CGA-------------------------------------CCCCCAGTCCCCTCCGCGGA`	`37 bp deleted → frameshift`
44	`CGAGCC--------------------------------------------CCTCCGCGGA`	`44 bp deleted → frameshift`
42	`CGAGCC---------------------------------------------CTCCGCGGA`	`45 bp deleted → no frameshift`
40	`CGAGCCACGGTCT----------------------------------------CCGCGGA`	`40 bp deleted → frameshift`
40	`CGAGCC----------------------------------------------TCCGCGGA`	`46 bp deleted → frameshift`
38	`CGAGCCACG-----------------------------------------------CGGA`	`47 bp deleted → frameshift`
36	`CGAGCCACGGTC------------------------------------------CGCGGA`	`42 bp deleted → no frameshift`
34	`CGAG-------------------------------------------TCCCCTCCGCGGA`	`43 bp deleted → frameshift`
34	`CGAGCC-------------------------------------------------GCGGA`	`49 bp deleted → frameshift`
29	`CGAGC----------------------------------------------------GGA`	`52 bp deleted → frameshift`
26	`CG------------------------------------------------------CGGA`	`54 bp deleted → no frameshift`
24	`CG--------------------------------------------------------GA`	`56 bp deleted → frameshift`
17	`CGA---------------------------------------------------------`	`57 bp deleted → no frameshift`

Version 5.01 - Documentation - Contact us - Downloads/local installation - Citation - License