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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314105_F | Primer3: not found at this Tm | N.d. | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314105_R | Primer3: not found at this Tm | N.d. | 1.00 |
| mm3_intergenic_5730559C18Rik|Gpr25/Gm26781_chr1_136234693_F | TCGTCGGCAGCGTCATTCAAACTCGCGCTTTCCC | 59.4 | 0.60 |
| mm3_intergenic_5730559C18Rik|Gpr25/Gm26781_chr1_136234693_R | GTCTCGTGGGCTCGGTCTGGACTTAAGAACCCTCCT | 57.4 | 0.60 |
| mm4_intergenic_Ythdf3|Gm26485_chr3_16554274_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_Ythdf3|Gm26485_chr3_16554274_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm3_intron_Bcas3_chr11_85501743_F | TCGTCGGCAGCGTCGGACTGGCTCATTTTGTTAACCA | 59.4 | 0.42 |
| mm3_intron_Bcas3_chr11_85501743_R | GTCTCGTGGGCTCGGGCAATTGGCACTGGGGAAAC | 60.3 | 0.42 |
| mm4_intergenic_Gm5513|Gm24319_chr19_15464600_F | TCGTCGGCAGCGTCGCAGCATCTTCATCTCACCC | 58.6 | 0.41 |
| mm4_intergenic_Gm5513|Gm24319_chr19_15464600_R | GTCTCGTGGGCTCGGAGAATGCTCACAAGAAGCCCT | 59.6 | 0.41 |
| mm4_intergenic_Gm8321|Inhbb_chr1_119299996_F | TCGTCGGCAGCGTCTAGTCACTGAAAGCACCGGC | 60.3 | 0.40 |
| mm4_intergenic_Gm8321|Inhbb_chr1_119299996_R | GTCTCGTGGGCTCGGGCCAAAAGCAGCTTTGCAATC | 59.8 | 0.40 |
| mm4_intergenic_Htatsf1|Vgll1_chrX_57077611_F | Primer3: not found at this Tm | N.d. | 0.39 |
| mm4_intergenic_Htatsf1|Vgll1_chrX_57077611_R | Primer3: not found at this Tm | N.d. | 0.39 |
| mm3_intron_Arhgef28_chr13_97935134_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm3_intron_Arhgef28_chr13_97935134_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Ak9_chr10_41365325_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intron_Ak9_chr10_41365325_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gse1|Gm26971_chr8_120517561_F | TCGTCGGCAGCGTCTTCAGCTCGGCTTCAACGAT | 60.0 | 0.33 |
| mm4_intergenic_Gse1|Gm26971_chr8_120517561_R | GTCTCGTGGGCTCGGCCCTCAGGCCTCTGCTCTAT | 60.4 | 0.33 |
| mm4_intergenic_Gm10485|Dnmt3a_chr12_3860822_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Gm10485|Dnmt3a_chr12_3860822_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Arid1a|Gm25270_chr4_133778515_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intergenic_Arid1a|Gm25270_chr4_133778515_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Sorcs2_chr5_36119391_F | TCGTCGGCAGCGTCCTGATGCTGAACTGTGCGTG | 59.8 | 0.31 |
| mm4_intron_Sorcs2_chr5_36119391_R | GTCTCGTGGGCTCGGCCTTCCTGGCCTCTTTCCTG | 60.0 | 0.31 |
| mm4_intergenic_Gm11499|Gm11500_chr11_92339574_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Gm11499|Gm11500_chr11_92339574_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_RP24-108J19.1|Lrrc6_chr15_66310297_F | TCGTCGGCAGCGTCTCTCAATTCGGAATGAACACATGA | 58.7 | 0.27 |
| mm4_intergenic_RP24-108J19.1|Lrrc6_chr15_66310297_R | GTCTCGTGGGCTCGGACCATCACAACTTGTATCAACCT | 58.0 | 0.27 |
| mm4_intergenic_Gm21833|Rpl21-ps5_chr16_82896497_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm21833|Rpl21-ps5_chr16_82896497_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Ero1lb_chr13_12600950_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Ero1lb_chr13_12600950_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm25804|Gpr137b_chr13_13344315_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm25804|Gpr137b_chr13_13344315_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Baz1a_chr12_54937772_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Baz1a_chr12_54937772_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_4921534H16Rik_chr9_98004018_F | TCGTCGGCAGCGTCAGGGCATTACGGTTCACAGG | 60.0 | 0.25 |
| mm4_intron_4921534H16Rik_chr9_98004018_R | GTCTCGTGGGCTCGGTTGTATAGCCTGCCAGAGGG | 58.8 | 0.25 |
| mm4_intron_8030443G20Rik_chr4_108963777_F | TCGTCGGCAGCGTCGCCATTAGAAACCCAGCCCT | 60.0 | 0.25 |
| mm4_intron_8030443G20Rik_chr4_108963777_R | GTCTCGTGGGCTCGGAACTGGGCAACTGAGAGGAG | 59.3 | 0.25 |
| mm4_intergenic_Magee2|Gm6285_chrX_104935583_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Magee2|Gm6285_chrX_104935583_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Lama1_chr17_67765239_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Lama1_chr17_67765239_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Irf2|Enpp6_chr8_46983580_F | TCGTCGGCAGCGTCGGAGCATCAGCCTTCCTCAG | 60.1 | 0.22 |
| mm4_intergenic_Irf2|Enpp6_chr8_46983580_R | GTCTCGTGGGCTCGGCTAACTGTGCCCTGTGCTCA | 59.9 | 0.22 |
| mm4_intergenic_Ythdc2|Gm26928_chr18_44921864_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Ythdc2|Gm26928_chr18_44921864_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_exon_AI661453_chr17_47469851_F | TCGTCGGCAGCGTCCCTAGCCGCTTGGAGATGAG | 59.9 | 0.19 |
| mm3_exon_AI661453_chr17_47469851_R | GTCTCGTGGGCTCGGCTTCTCAAGGCCTCCTCGTG | 60.1 | 0.19 |
| mm4_intron_Dexi_chr16_10539666_F | TCGTCGGCAGCGTCGTGTCCTGGTCTGGCTTGTT | 60.1 | 0.18 |
| mm4_intron_Dexi_chr16_10539666_R | GTCTCGTGGGCTCGGAGGATGCAGGAGGGATGACT | 60.0 | 0.18 |
| mm4_intergenic_4933423P22Rik|Ptprj_chr2_90503637_F | TCGTCGGCAGCGTCCACTCAGAGGCTGTCAACCC | 60.3 | 0.18 |
| mm4_intergenic_4933423P22Rik|Ptprj_chr2_90503637_R | GTCTCGTGGGCTCGGTTCTCTGCAGTGCATGGGTT | 59.8 | 0.18 |
| mm4_intron_Gm21976_chr13_98305495_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Gm21976_chr13_98305495_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intron_Src_chr2_157433737_F | TCGTCGGCAGCGTCGAGCTGGGACTGACAAGGTC | 60.0 | 0.17 |
| mm4_intron_Src_chr2_157433737_R | GTCTCGTGGGCTCGGCATAGGTCACCTTGGCCCTG | 60.1 | 0.17 |
| mm3_exon_Rassf10_chr7_112954645_F | TCGTCGGCAGCGTCGAAGCGTCGCTGCCCAAC | 62.0 | 0.16 |
| mm3_exon_Rassf10_chr7_112954645_R | GTCTCGTGGGCTCGGCTGCTTCTCCTGGGTCAAGG | 60.0 | 0.16 |
| mm4_intergenic_Gm15917|Tle2_chr10_81574165_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm15917|Tle2_chr10_81574165_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Lrpap1|Adra2c_chr5_35274705_F | TCGTCGGCAGCGTCGTTTGTGCCTCAACAGCAGG | 59.9 | 0.16 |
| mm4_intergenic_Lrpap1|Adra2c_chr5_35274705_R | GTCTCGTGGGCTCGGTCCCGTGACCAAGAGGAGAT | 59.9 | 0.16 |
| mm4_intron_Stk10_chr11_32574446_F | TCGTCGGCAGCGTCAATGGGCAGATGGACACACC | 60.3 | 0.15 |
| mm4_intron_Stk10_chr11_32574446_R | GTCTCGTGGGCTCGGATGATGGCATCCACAGCTCC | 60.1 | 0.15 |
| mm4_intron_Dcbld2_chr16_58429977_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Dcbld2_chr16_58429977_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Map7d2_chrX_159415104_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Map7d2_chrX_159415104_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Tnfrsf19_chr14_61031462_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Tnfrsf19_chr14_61031462_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Fam102a_chr2_32547450_F | TCGTCGGCAGCGTCGCTCAGTGGCTTGCTTGAAG | 59.7 | 0.14 |
| mm4_intron_Fam102a_chr2_32547450_R | GTCTCGTGGGCTCGGGGTCAGACACCACCATGAGG | 60.0 | 0.14 |
| mm4_intergenic_Gm24632|Lyzl1_chr18_4008898_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm24632|Lyzl1_chr18_4008898_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm25528|Gm23176_chr9_87963382_F | TCGTCGGCAGCGTCCAGCCAAGGGAAGAGGATCC | 59.8 | 0.14 |
| mm4_intergenic_Gm25528|Gm23176_chr9_87963382_R | GTCTCGTGGGCTCGGGGCAAGTGCAATAGGTCCCT | 60.0 | 0.14 |
| mm4_intergenic_Fam129a|Edem3_chr1_151750007_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Fam129a|Edem3_chr1_151750007_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intron_Igsf3_chr3_101416407_F | TCGTCGGCAGCGTCTTTTGCAGGCCTTGGGAGAA | 60.1 | 0.13 |
| mm4_intron_Igsf3_chr3_101416407_R | GTCTCGTGGGCTCGGGATGCTCAACGTGGCCTCTA | 59.8 | 0.13 |
| mm4_intergenic_9330185C12Rik|Cntnap5a_chr1_114818593_F | TCGTCGGCAGCGTCGGAGTGACAGAACCAGGAAGG | 59.9 | 0.13 |
| mm4_intergenic_9330185C12Rik|Cntnap5a_chr1_114818593_R | GTCTCGTGGGCTCGGGCAGTTGGTCAGAGGCAAAG | 59.4 | 0.13 |
| mm4_intergenic_Ntf3|Kcna5_chr6_126284262_F | TCGTCGGCAGCGTCGCCCTTCTCAGGAGTGTTCC | 60.0 | 0.13 |
| mm4_intergenic_Ntf3|Kcna5_chr6_126284262_R | GTCTCGTGGGCTCGGTGAGGGAGCAATAAACGGGT | 59.0 | 0.13 |
| mm4_intron_Pomp_chr5_147867554_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Pomp_chr5_147867554_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Top1mt|Rhpn1_chr15_75678876_F | TCGTCGGCAGCGTCCCACGCCCTTCTGACAGAG | 60.0 | 0.12 |
| mm4_intergenic_Top1mt|Rhpn1_chr15_75678876_R | GTCTCGTGGGCTCGGACTTCCTCAGGTTGCCATGG | 59.9 | 0.12 |
| mm4_exon_Gm11729_chr11_117414631_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Gm11729_chr11_117414631_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Ptprm_chr17_67159561_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Ptprm_chr17_67159561_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Smyd1|mmu-mir-8112_chr6_71270352_F | TCGTCGGCAGCGTCTGAAGCCTTTTACGGACGGA | 59.3 | 0.11 |
| mm4_intergenic_Smyd1|mmu-mir-8112_chr6_71270352_R | GTCTCGTGGGCTCGGGGCCCTGCTTCCGGTTAATA | 59.8 | 0.11 |
| mm4_intron_1700047F07Rik_chr4_107721119_F | TCGTCGGCAGCGTCAATCAGTCAGCACAGGGGTC | 59.6 | 0.11 |
| mm4_intron_1700047F07Rik_chr4_107721119_R | GTCTCGTGGGCTCGGGAGTAAGACCTGGGCTGAGC | 59.8 | 0.11 |
| mm4_intron_Supt5_chr7_28329702_F | TCGTCGGCAGCGTCGAGTCACAAGGGGAGTCAGG | 59.3 | 0.11 |
| mm4_intron_Supt5_chr7_28329702_R | GTCTCGTGGGCTCGGTGAGGAGGACAGAGGATGGG | 60.0 | 0.11 |
| mm4_intergenic_Lrrc58/Gm15725|Gpr156_chr16_37907701_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Lrrc58/Gm15725|Gpr156_chr16_37907701_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm11650|Gm23120_chr11_107820374_F | TCGTCGGCAGCGTCCTACCACACAGAGGCAGCTG | 60.3 | 0.11 |
| mm4_intergenic_Gm11650|Gm23120_chr11_107820374_R | GTCTCGTGGGCTCGGTTTAGCAGGTGAGCGCCATT | 60.3 | 0.11 |
| mm4_intergenic_Gm11631|Fbxl20/Gm11632_chr11_98077825_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm11631|Fbxl20/Gm11632_chr11_98077825_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Pcsk2|Gm12360/Pcsk2_chr2_143732856_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Pcsk2|Gm12360/Pcsk2_chr2_143732856_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm25509|n-R5-8s1_chr18_73097826_F | TCGTCGGCAGCGTCGCACAGGTTAGCTGGAGGAT | 59.4 | 0.10 |
| mm4_intergenic_Gm25509|n-R5-8s1_chr18_73097826_R | GTCTCGTGGGCTCGGCTCAAGATAGAGCTGCTGCCA | 59.8 | 0.10 |
| mm4_intergenic_Gm24254|4933436C20Rik_chr8_92275385_F | TCGTCGGCAGCGTCCTCTGACTCCACAGGGCAAG | 60.0 | 0.09 |
| mm4_intergenic_Gm24254|4933436C20Rik_chr8_92275385_R | GTCTCGTGGGCTCGGACTCGGGGTAGATGTGTGGA | 59.9 | 0.09 |
| mm4_intron_Slc25a26_chr6_94589227_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Slc25a26_chr6_94589227_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm23763|Gm25609_chr1_137166119_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm23763|Gm25609_chr1_137166119_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_9030624J02Rik_chr7_118833987_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_9030624J02Rik_chr7_118833987_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Tmc5_chr7_118658833_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Tmc5_chr7_118658833_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_72121295_F | TCGTCGGCAGCGTCGGGCAGAAGGATGAGGCTAA | 59.1 | 0.08 |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_72121295_R | GTCTCGTGGGCTCGGCCTCCCTTTTCTCCAGGCTT | 59.2 | 0.08 |
| mm4_intron_Lyrm4_chr13_36025213_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Lyrm4_chr13_36025213_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Tnnt2|Pkp1_chr1_135857470_F | TCGTCGGCAGCGTCAGCAGCCACACCTTAAGCTC | 60.3 | 0.08 |
| mm4_intergenic_Tnnt2|Pkp1_chr1_135857470_R | GTCTCGTGGGCTCGGCCTGCCCACTGCTTTCCTTA | 59.9 | 0.08 |
| mm4_intergenic_Gm25037|Cdk14_chr5_4874655_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm25037|Cdk14_chr5_4874655_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Ddx31|Barhl1_chr2_28906865_F | TCGTCGGCAGCGTCTGTCCTCCACTCCCACAGAA | 60.1 | 0.08 |
| mm4_intergenic_Ddx31|Barhl1_chr2_28906865_R | GTCTCGTGGGCTCGGGCCTAACCAGTGGCAGCTTA | 60.0 | 0.08 |
| mm4_intergenic_Nsmce1|Il4ra_chr7_125551321_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Nsmce1|Il4ra_chr7_125551321_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Itgb5_chr16_33832166_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Itgb5_chr16_33832166_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22251|Zfp521_chr18_13674203_F | TCGTCGGCAGCGTCACAACATTCTACACAGGACTGTCT | 59.6 | 0.07 |
| mm4_intergenic_Gm22251|Zfp521_chr18_13674203_R | GTCTCGTGGGCTCGGTCGGTCTACATTTTCTCCTCATCC | 59.9 | 0.07 |
| mm4_exon_Cux1/Gm16599_chr5_136275085_F | TCGTCGGCAGCGTCGGGCAGTGGCTGAGGTAG | 59.4 | 0.07 |
| mm4_exon_Cux1/Gm16599_chr5_136275085_R | GTCTCGTGGGCTCGGGACAGGACGACGGTGAAGAC | 60.3 | 0.07 |
| mm4_intron_Lrrfip1_chr1_91098607_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Lrrfip1_chr1_91098607_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intergenic_9230114K14Rik|Gm25601_chr5_52267759_F | TCGTCGGCAGCGTCTCTCTTCGGTCTCTCCTCTCC | 59.7 | 0.07 |
| mm3_intergenic_9230114K14Rik|Gm25601_chr5_52267759_R | GTCTCGTGGGCTCGGTGCCCCTCTCACTCTTCCTT | 60.1 | 0.07 |
| mm4_intron_Lif_chr11_4264053_F | TCGTCGGCAGCGTCTCCTGGAGAGAAGCACTGGA | 59.8 | 0.06 |
| mm4_intron_Lif_chr11_4264053_R | GTCTCGTGGGCTCGGCCTGCTAGGGGTTTGACAGG | 60.0 | 0.06 |
| mm4_intron_Ddx31_chr2_28896491_F | TCGTCGGCAGCGTCTCCCACTCCCCTCTTAACCC | 60.2 | 0.06 |
| mm4_intron_Ddx31_chr2_28896491_R | GTCTCGTGGGCTCGGGCAGGTTTGAGAGGTGAGGG | 60.3 | 0.06 |
| mm4_intergenic_Gm13446/Dab2ip|Dab2ip_chr2_35571444_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm13446/Dab2ip|Dab2ip_chr2_35571444_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Itpk1|Gm21971/Itpk1_chr12_102640318_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Itpk1|Gm21971/Itpk1_chr12_102640318_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Zfp180_chr7_24084926_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Zfp180_chr7_24084926_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Herpud2_chr9_25151466_F | TCGTCGGCAGCGTCGCAGAGCGAGCTTGAAGAGA | 60.1 | 0.06 |
| mm4_exon_Herpud2_chr9_25151466_R | GTCTCGTGGGCTCGGGCGGCCTTCTTTCCTTCAGG | 61.3 | 0.06 |
| mm4_intergenic_Gm26190|B3gat1_chr9_26574011_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm26190|B3gat1_chr9_26574011_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm13358|Egfl7_chr2_26562674_F | TCGTCGGCAGCGTCAGCACCAGGAAAGCAGTGTT | 60.1 | 0.05 |
| mm4_intergenic_Gm13358|Egfl7_chr2_26562674_R | GTCTCGTGGGCTCGGCTCTCCCCATCTCATGATGCC | 59.9 | 0.05 |
| mm4_exon_Gm6277/Cables1/Mir1901_chr18_11839637_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Gm6277/Cables1/Mir1901_chr18_11839637_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Tbx3/Gm2301_chr5_119672045_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Tbx3/Gm2301_chr5_119672045_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Amacr_chr15_10981835_F | TCGTCGGCAGCGTCAGATAAACTGTGGCTCGGGC | 60.1 | 0.04 |
| mm4_exon_Amacr_chr15_10981835_R | GTCTCGTGGGCTCGGGGACCATTCCGCAGAACG | 58.5 | 0.04 |
| mm4_exon_Ing4_chr6_125047395_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Ing4_chr6_125047395_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm7882|Gm5292_chr5_43920625_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm7882|Gm5292_chr5_43920625_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Fbxw7|Mir466k_chr3_85174220_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Fbxw7|Mir466k_chr3_85174220_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gm13849_chr6_31840162_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gm13849_chr6_31840162_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm21897|Gm6031_chr16_15386929_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm21897|Gm6031_chr16_15386929_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Anapc10_chr8_79731040_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Anapc10_chr8_79731040_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Sec16b|Brinp2_chr1_157726316_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Sec16b|Brinp2_chr1_157726316_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Prkcz_chr4_155265795_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Prkcz_chr4_155265795_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Mettl5_chr2_69881296_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Mettl5_chr2_69881296_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm2_exon_Ppp3cb_chr14_20546429_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm2_exon_Ppp3cb_chr14_20546429_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Pja1|Gm14816_chrX_99629645_F | TCGTCGGCAGCGTCGGGAGAAATCCAGGCAGCTT | 60.0 | 0.02 |
| mm4_intergenic_Pja1|Gm14816_chrX_99629645_R | GTCTCGTGGGCTCGGGCCTCTGACTCCCTAAACAGT | 59.0 | 0.02 |
| mm3_intergenic_Arl14|Ppm1l_chr3_69283205_F | TCGTCGGCAGCGTCCCTGGGTGTGGTTGCTAGAA | 59.6 | 0.02 |
| mm3_intergenic_Arl14|Ppm1l_chr3_69283205_R | GTCTCGTGGGCTCGGCCTAGCAGTTCAGGGGATGC | 60.1 | 0.02 |
| mm3_intergenic_Ergic1|Gm22420_chr17_26576998_F | TCGTCGGCAGCGTCCATGCACCCCTGCTTGAGA | 60.0 | 0.02 |
| mm3_intergenic_Ergic1|Gm22420_chr17_26576998_R | GTCTCGTGGGCTCGGAAGCTGAGCTTCAGAGAGCG | 60.1 | 0.02 |
| mm4_intergenic_Gm15969|Casz1_chr4_148918563_F | TCGTCGGCAGCGTCCCAAGGCGAGGAGAAGGATG | 60.1 | 0.02 |
| mm4_intergenic_Gm15969|Casz1_chr4_148918563_R | GTCTCGTGGGCTCGGGATTTGTGCCCAAGATCCGC | 59.8 | 0.02 |
| mm4_intergenic_Kcnb2|Gm25227_chr1_15401747_F | TCGTCGGCAGCGTCGCACAACATGGAGTGGAAGC | 59.7 | 0.02 |
| mm4_intergenic_Kcnb2|Gm25227_chr1_15401747_R | GTCTCGTGGGCTCGGTGTACTGCAGCTCACTCAACA | 59.5 | 0.02 |
| mm2_intergenic_Fbxl5|Bst1_chr5_43782501_F | TCGTCGGCAGCGTCGGTTTTGCTAGGCCTGGGAT | 60.0 | 0.02 |
| mm2_intergenic_Fbxl5|Bst1_chr5_43782501_R | GTCTCGTGGGCTCGGCATCCCACTCTGCTCCGTG | 60.1 | 0.02 |
| mm4_exon_Pde4a_chr9_21181798_F | TCGTCGGCAGCGTCCTTCCGAGGCAGTGTACAGG | 60.1 | 0.02 |
| mm4_exon_Pde4a_chr9_21181798_R | GTCTCGTGGGCTCGGAACCCGTGTCCTGAGAGGT | 60.1 | 0.02 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36663621_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36663621_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Fbln2_chr6_91233135_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Fbln2_chr6_91233135_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_St8sia2_chr7_73944261_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intron_St8sia2_chr7_73944261_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm7180|Pde8a_chr7_81280150_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm7180|Pde8a_chr7_81280150_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_1700015G11Rik|Gm22211_chr7_52035909_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_1700015G11Rik|Gm22211_chr7_52035909_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Lrp1_chr10_127554518_F | TCGTCGGCAGCGTCAAGGAGACAACCATGGCCTG | 59.9 | 0.01 |
| mm4_intron_Lrp1_chr10_127554518_R | GTCTCGTGGGCTCGGACGGCTCCAACTACACACTG | 59.9 | 0.01 |
| mm4_intergenic_Gm9855|Foxo3_chr10_42108053_F | TCGTCGGCAGCGTCTCCAGGCCATAACCCTCTCA | 59.9 | 0.01 |
| mm4_intergenic_Gm9855|Foxo3_chr10_42108053_R | GTCTCGTGGGCTCGGCCTTGGTTGCATCAGAAGTGC | 60.0 | 0.01 |
| mm4_intron_Hsf1_chr15_76480992_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Hsf1_chr15_76480992_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm23106|1700029M20Rik_chr4_135592187_F | TCGTCGGCAGCGTCTTGCTGGAACCAGGCAGTAG | 59.9 | 0.01 |
| mm4_intergenic_Gm23106|1700029M20Rik_chr4_135592187_R | GTCTCGTGGGCTCGGGTCTCTTTGGTACCGCCCTC | 60.1 | 0.01 |
| mm4_intron_Myo1d_chr11_80692201_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Myo1d_chr11_80692201_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Dock9_chr14_121691512_F | TCGTCGGCAGCGTCCAGTCAGGACCGTGGGATG | 59.7 | 0.01 |
| mm4_intron_Dock9_chr14_121691512_R | GTCTCGTGGGCTCGGCTGGCACTGTGCTAAGGAGG | 60.3 | 0.01 |
| mm4_intergenic_Ak8|Mir3088_chr2_28726440_F | TCGTCGGCAGCGTCTCTGGATCAGAATCAGGTGAGT | 58.2 | 0.01 |
| mm4_intergenic_Ak8|Mir3088_chr2_28726440_R | GTCTCGTGGGCTCGGCTCATCCCTCTGCTGGCAC | 60.1 | 0.01 |
| mm4_intron_Ncor2_chr5_125141445_F | TCGTCGGCAGCGTCGAGAAGCCCTCTCCCTCCTT | 60.3 | 0.01 |
| mm4_intron_Ncor2_chr5_125141445_R | GTCTCGTGGGCTCGGACGGGAGCATTTTCCTCTGG | 60.0 | 0.01 |
| mm4_intergenic_Gtf2h1|Ldha_chr7_46835220_F | TCGTCGGCAGCGTCCGCCATCCCCTAGTGAGTTC | 59.8 | 0.01 |
| mm4_intergenic_Gtf2h1|Ldha_chr7_46835220_R | GTCTCGTGGGCTCGGAAACTTACAGGGCGAGGTGC | 60.6 | 0.01 |
| mm4_intron_Tax1bp1_chr6_52714053_F | TCGTCGGCAGCGTCCCGCGATTGTGACGTGTTTT | 60.0 | 0.01 |
| mm4_intron_Tax1bp1_chr6_52714053_R | GTCTCGTGGGCTCGGCCGTCCCTGGCTTGTTAGAC | 60.3 | 0.01 |
| mm4_intron_Tiam1_chr16_89954674_F | TCGTCGGCAGCGTCCTGCTGGGCATGTCAGAGG | 60.4 | 0.01 |
| mm4_intron_Tiam1_chr16_89954674_R | GTCTCGTGGGCTCGGGGCATTCCTGGTCCTGTTGT | 60.2 | 0.01 |
| mm4_intergenic_Dlgap1|5031415H12Rik_chr17_70729224_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Dlgap1|5031415H12Rik_chr17_70729224_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Clybl|Gm5089_chr14_122406779_F | TCGTCGGCAGCGTCGGTGTAGCAGTGCTGGGG | 60.0 | 0.00 |
| mm4_intergenic_Clybl|Gm5089_chr14_122406779_R | GTCTCGTGGGCTCGGATCTCGCACCATTACAGCCC | 60.1 | 0.00 |
| mm4_intergenic_AL808027.1|Prrc2b_chr2_32175864_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_AL808027.1|Prrc2b_chr2_32175864_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pex5l|AC113989.1_chr3_33217856_F | TCGTCGGCAGCGTCAGAACTGAGACTCACACGCG | 60.0 | 0.00 |
| mm4_intergenic_Pex5l|AC113989.1_chr3_33217856_R | GTCTCGTGGGCTCGGGAGGGGAGGTGAACTTGGTG | 59.9 | 0.00 |
| mm4_exon_1810013L24Rik_chr16_8830530_F | TCGTCGGCAGCGTCCCGGAGCATTGGTTCTCCA | 59.7 | 0.00 |
| mm4_exon_1810013L24Rik_chr16_8830530_R | GTCTCGTGGGCTCGGGATGCCACAGCTTCTGTTGC | 60.1 | 0.00 |
| mm4_intron_Cdh13_chr8_118639810_F | TCGTCGGCAGCGTCGAGTCGTGAGTCTCAGGCAG | 59.8 | 0.00 |
| mm4_intron_Cdh13_chr8_118639810_R | GTCTCGTGGGCTCGGTTGGCTCCAGACAAGGGAAC | 59.8 | 0.00 |
| mm4_intergenic_Mir9-3|AI854517_chr7_79505447_F | TCGTCGGCAGCGTCCCAGGGATTCCAGCTCGAC | 59.8 | 0.00 |
| mm4_intergenic_Mir9-3|AI854517_chr7_79505447_R | GTCTCGTGGGCTCGGGAGGTGAAGGGAATGCCTCC | 60.1 | 0.00 |
| mm4_intron_9930021J03Rik_chr19_29805122_F | TCGTCGGCAGCGTCGGCTACCTTCTCGGCTTTGG | 60.7 | 0.00 |
| mm4_intron_9930021J03Rik_chr19_29805122_R | GTCTCGTGGGCTCGGTGAGTTGGTGCTAGAACCGC | 60.3 | 0.00 |
| mm4_intron_Th_chr7_142927564_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Th_chr7_142927564_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pax5_chr4_44611415_F | TCGTCGGCAGCGTCTGCTCCCTTGCACAGGTAGA | 60.8 | 0.00 |
| mm4_intron_Pax5_chr4_44611415_R | GTCTCGTGGGCTCGGCCATAGCTGGCCCAAGAACT | 59.7 | 0.00 |
| mm4_intron_Wdr19_chr5_65203836_F | TCGTCGGCAGCGTCATCTTCGGCCGTTGGGAAAT | 60.0 | 0.00 |
| mm4_intron_Wdr19_chr5_65203836_R | GTCTCGTGGGCTCGGATGAGGGAAAGCACAGAGGG | 59.3 | 0.00 |
| mm4_intron_Bag3_chr7_128531361_F | TCGTCGGCAGCGTCTCGTCAGGTCTGTAGGAGGG | 60.0 | 0.00 |
| mm4_intron_Bag3_chr7_128531361_R | GTCTCGTGGGCTCGGACTGCCAGCCACAAGAGAAA | 59.8 | 0.00 |
| mm4_intergenic_Haghl|Narfl/mmu-mir-6966_chr17_25780172_F | TCGTCGGCAGCGTCTTAATCTCCTGGGCTGCAGC | 60.1 | 0.00 |
| mm4_intergenic_Haghl|Narfl/mmu-mir-6966_chr17_25780172_R | GTCTCGTGGGCTCGGAGCTGAGAAGTGTGCGATGT | 59.6 | 0.00 |
| mm4_intergenic_Gm8825|Gm25176_chr19_25851715_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm8825|Gm25176_chr19_25851715_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm5083|Gm27007_chr13_44318567_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm5083|Gm27007_chr13_44318567_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22302|Uri1_chr7_37839380_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22302|Uri1_chr7_37839380_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Rab40b_chr11_121357153_F | TCGTCGGCAGCGTCCCTGACTTTCCGGAAGCTGT | 59.9 | 0.00 |
| mm4_exon_Rab40b_chr11_121357153_R | GTCTCGTGGGCTCGGGTCGCCTTAAGAAGCCACCT | 60.0 | 0.00 |
| mm4_exon_Nsmf_chr2_25054451_F | TCGTCGGCAGCGTCCTCTCCTACAGTGGCTGGAC | 59.1 | 0.00 |
| mm4_exon_Nsmf_chr2_25054451_R | GTCTCGTGGGCTCGGCTAGGTCTCAGCCTCAGGGT | 60.0 | 0.00 |
| mm4_exon_Cd200_chr16_45400165_F | TCGTCGGCAGCGTCCCCACTGTTCCAAGGCTGAT | 59.9 | 0.00 |
| mm4_exon_Cd200_chr16_45400165_R | GTCTCGTGGGCTCGGGATACCCCGTTCTTCCTCGG | 59.6 | 0.00 |
| mm3_intergenic_Kif27|mmu-mir-6369/Kif27_chr13_58311514_F | TCGTCGGCAGCGTCTGCCTACAAAGCACTCTCTGT | 59.3 | 0.00 |
| mm3_intergenic_Kif27|mmu-mir-6369/Kif27_chr13_58311514_R | GTCTCGTGGGCTCGGTAGTCACAGGGAGCTGACCA | 59.8 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| mm3_intergenic_5730559C18Rik|Gpr25/Gm26781_chr1_136234693 |
ATTCAAACTCGCGCTTTCCCCTTTTTCCGTTGGTTCTGTTTACATTGGTACTGTCCTTGCCAGGGCCAAGCATGGCAGTT CAAAGTTCAGACTCCTTTACTAGGAGGGTTCTTAAGTCCAGA |
| mm3_intron_Bcas3_chr11_85501743 |
GGACTGGCTCATTTTGTTAACCAATAGCCTTTGTAGACAATATTAATTGAGCTGGCATGCTTGGCCCAGGTTCACTGTCT TTCTCTGATTCAGAGATAGGCAAGACAAATGTCCATTTTTGTTTCCCCAGTGCCAATTGC |
| mm4_intergenic_Gm5513|Gm24319_chr19_15464600 |
GCAGCATCTTCATCTCACCCAGACTCATGTGTATCCTCCTGGTCCAAGCATGGCTGTTTCCCTTGGGTTATAATAGTCTT CTCTATAAAAGGGCTTCTTGTGAGCATTCT |
| mm4_intergenic_Gm8321|Inhbb_chr1_119299996 |
TAGTCACTGAAAGCACCGGCCATGTTGGGCCCGCCCATCCTGGGCCAAGCTTTGCCGCACTTCCAGTTGACCATCCTGTC TCTTTAGATACTAGACCTCATCAGCAAAACAAAGATTGCAAAGCTGCTTTTGGC |
| mm4_intergenic_Gse1|Gm26971_chr8_120517561 |
TTCAGCTCGGCTTCAACGATGGATGCAGCCTGGACCAAGCATGGCTTCTCTTACTGATGTGGTTTATAGAGCAGAGGCCT GAGGG |
| mm4_intron_Sorcs2_chr5_36119391 |
CTGATGCTGAACTGTGCGTGGCAGTGTTGACCCCACCCTGGACCAAGCATGGCCACTTTCTTTCCACCAAGAAGAAATAC ATGCTGCATAACGACATTGCAGGAAAGAGGCCAGGAAGG |
| mm4_intergenic_RP24-108J19.1|Lrrc6_chr15_66310297 |
TCTCAATTCGGAATGAACACATGAAAATTAATCTTCAGAGGGAGCAGTCCTGCTTGACCCAGGTTAAAGGTAAACATCTT TGGTAAGGTTGATACAAGTTGTGATGGT |
| mm4_intron_4921534H16Rik_chr9_98004018 |
AGGGCATTACGGTTCACAGGGTTGATATCAGATAGCATCTTGCAAGGGAGTTGTCTACTCGAGGAAGGGTCATGCTTGGA CCGGGGAAGACCCTCTGGCAGGCTATACAA |
| mm4_intron_8030443G20Rik_chr4_108963777 |
GCCATTAGAAACCCAGCCCTCATTGCAATAACCAGGACACACACCAGGGCCAAGCATCGCCGCAATCCTGGCAAGGAAAA ACGGAGCAGAATACTCCCCTCCTCTCAGTTGCCCAGTT |
| mm4_intergenic_Irf2|Enpp6_chr8_46983580 |
GGAGCATCAGCCTTCCTCAGTGCCCCTGGCCAAGCATGTCTGCACCTCATGGCCACCAATTTCCCTGAGCACAGGGCACA GTTAG |
| mm3_exon_AI661453_chr17_47469851 |
CCTAGCCGCTTGGAGATGAGTTTATACCATTTGCACAACACAGGGCTCTGCCTCAGGAGCCTTATGTACCTGCCAGGGCC AAGCAGGGCCATTCCCCCTTGGCACTTGCTCACGAGGAGGCCTTGAGAAG |
| mm4_intron_Dexi_chr16_10539666 |
GTGTCCTGGTCTGGCTTGTTAAAACATGACTGGGAGCAAGCATGCCTGGCCCAGGGCTCCTGTGTTTGGAAAGGGTCAAG AGGCAAGGTTGGTCACTTCATTCTAGGTTTGTATCAGTCATCCCTCCTGCATCCT |
| mm4_intergenic_4933423P22Rik|Ptprj_chr2_90503637 |
CACTCAGAGGCTGTCAACCCCCAGGGCCAAGCACTCCCCCTCCATCCTCCCCCTCTCACCTCTGCAACCCATGCACTGCA GAGAA |
| mm4_intron_Src_chr2_157433737 |
GAGCTGGGACTGACAAGGTCTGGAACCAGGCTACTGAGGGTAGGAAACCAAGGCTAAGCATGGCTTCTCCATGGCCAGGA TCACAGTGGGGCAGGGCTAGGTTTGGGCTCTCAGGGCCAAGGTGACCTATG |
| mm3_exon_Rassf10_chr7_112954645 |
GAAGCGTCGCTGCCCAACGCCGGACCGCGCAGCGCCGAGGCGCGGGTAGTGCTCAGCCGCGAGCGCCCCTGCTTGGCCCG GGGCGCCCCAGCACGGCCCAGCCTGGCCTTGACCCAGGAGAAGCAG |
| mm4_intergenic_Lrpap1|Adra2c_chr5_35274705 |
GTTTGTGCCTCAACAGCAGGAAGCCTGGCCCAAGGGTGGCTCCAGGGCCATTCATGGTGGCTCCAAAATAGAAGCAATGG GGCATATACTCTTTCTTCAGTGACTTGTATCCTTGTCCTCATCTCCTCTTGGTCACGGGA |
| mm4_intron_Stk10_chr11_32574446 |
AATGGGCAGATGGACACACCCCCAGCAGGAGGGGTGGATGGAGCAGGCAGGCATGGCCCCGGCTCATTGTTCTGGTTTGT GTTTCCTACAGATCATGATTGAGTTCTGCCCTGGGGGAGCTGTGGATGCCATCAT |
| mm4_intron_Fam102a_chr2_32547450 |
GCTCAGTGGCTTGCTTGAAGTTAGTGGCAGTGGCAATGATTGGCCCAGGAGTGGTCTTGAGTCTGCAGTCTCAGGTGTCT ATGGAGGAGGTGGAGCCCTGTGAGTCAGGGCCTCATGGTGGTGTCTGACC |
| mm4_intergenic_Gm25528|Gm23176_chr9_87963382 |
CAGCCAAGGGAAGAGGATCCAAATATGATCCTTCTCACTCCAGGTCCAAGCATGTGCCCTCCCTCTCACCCCAAAAACAC TGTCCTGGTCTAATCAAGCAGTTCAGGAAGGCATAAAGGGACCTATTGCACTTGCC |
| mm4_intron_Igsf3_chr3_101416407 |
TTTTGCAGGCCTTGGGAGAAGCCCTTCTTGTTCTGCTGCCTCTCCCCTACCTCGGGAAGCACCCCAGCTTGTGTTCAGCC TGGGCCAAGCCTGGCAGCCTCCTTTAGAGGCCACGTTGAGCATC |
| mm4_intergenic_9330185C12Rik|Cntnap5a_chr1_114818593 |
GGAGTGACAGAACCAGGAAGGAGTGAGGTAGAAATGTGAGCACAAGTTGAGGCCCAGGTCCTAGCTTGGCCTCTCCTCTT TGCCTCTGACCAACTGC |
| mm4_intergenic_Ntf3|Kcna5_chr6_126284262 |
GCCCTTCTCAGGAGTGTTCCTTCCCTGGCCAAGCATGCGCCCTCCTTATCCATACTGGTACATAGGACCTCCTCTCCCCC ATCTTCTCTTCTTCCTTTACCCGTTTATTGCTCCCTCA |
| mm4_intergenic_Top1mt|Rhpn1_chr15_75678876 |
CCACGCCCTTCTGACAGAGCTCCGCCCCCACACGTCCAGGAGTCCCGCCCTGCCCGCCCACCTTGGGAAGGCGTCCCCAG CCAGGGCCGAGCATTGCCCCTCTCCCAGCGCTTCTACCTGCCATGGCAACCTGAGGAAGT |
| mm4_intergenic_Smyd1|mmu-mir-8112_chr6_71270352 |
TGAAGCCTTTTACGGACGGAGTTTATTAGGCAGTCGGGGAGTGGCAATGCTTGGCAACGGAAGCAAGTGCAGCCTGTTGA CTTACACAGTAGTGTAGTATTAACCGGAAGCAGGGCC |
| mm4_intron_1700047F07Rik_chr4_107721119 |
AATCAGTCAGCACAGGGGTCTGGAGGCCCCAGGCTTGGCCCAGGCAACCCTTCCTGATGAGCAGATCTGTAGTCATCTCT GGGCTCAGCCCAGGTCTTACTC |
| mm4_intron_Supt5_chr7_28329702 |
GAGTCACAAGGGGAGTCAGGCCGGGAACCAGCTTGGGGAGTTGAGGGGACATGCCTGGCCCTGGGGAGATGGCTAAGGCT CCCCCATCCTTCCCCCATCCTCTGTCCTCCTCA |
| mm4_intergenic_Gm11650|Gm23120_chr11_107820374 |
CTACCACACAGAGGCAGCTGGGCAGACCTGGGGCAAGGAAGGCTGCTCCACAGGGGAAGAACTCTGTGAGACATCATTGT GATTTCAGGTTAATCAGAGAGAGAACTAAACAGGCCCAATGGCGCTCACCTGCTAAA |
| mm4_intergenic_Gm25509|n-R5-8s1_chr18_73097826 |
GCACAGGTTAGCTGGAGGATTCTTGAGATTAAGAAAATTAGCAGCCTGGAGCAAGAGGCCTGGCAAGCTGCAGGGAGCCC CCATTCTAGGCCCAGGAGAGGCTTGGCAGCAGCTCTATCTTGAG |
| mm4_intergenic_Gm24254|4933436C20Rik_chr8_92275385 |
CTCTGACTCCACAGGGCAAGTGAGGTGGAGCCTGCCAGAGGGCCTGAGCCTAGCACGGCCACTCCTGGCTCTCTCGGGCT CCCTGCCTATCAGGGGTGTACCCACCTACTCCCATATCCACACATCTACCCCGAGT |
| mm4_intergenic_Irx1|Rpl9-ps4_chr13_72121295 |
GGGCAGAAGGATGAGGCTAATCTCCACATTTAGTCCTTCTGTTTGTGTGGACCTGCCTTGCCTGGCCCTGGCAGATCAAA GGAAGTTGGCAAAAATCAGATTAAGCCTGGAGAAAAGGGAGG |
| mm4_intergenic_Tnnt2|Pkp1_chr1_135857470 |
AGCAGCCACACCTTAAGCTCATGCCAATCTCAAAGTAGTTTCCTCTGAAAAGTCAGGCCTGGGCAACGTAGAACCTGGGC CAGGCAGGGCTGCTCTTAAGGAAAGCAGTGGGCAGG |
| mm4_intergenic_Ddx31|Barhl1_chr2_28906865 |
TGTCCTCCACTCCCACAGAAGCCAGGGCCAAGCATAGCCCAGCCGAGGACATTCATGGTAGCCCGAAGGGCTGCTGATTC TCTCCTGGCACCTCTCAGAGCAGGTGGTTGTAAGCTGCCACTGGTTAGGC |
| mm4_intergenic_Gm22251|Zfp521_chr18_13674203 |
ACAACATTCTACACAGGACTGTCTGTGGAGGTAGGGGCCAGGCTTGGCCACGGGTAGAAGGATGAGGAGAAAATGTAGAC CGA |
| mm4_exon_Cux1/Gm16599_chr5_136275085 |
GGGCAGTGGCTGAGGTAGCGGCGTCCTCTCCGGCCGCGGGTGCGGCGAGGCTTGGCACAGGCGCTGGGGCGTCGGCGAGG CCCTCGGGAAGTGGCCTAGGCCGGCTTGCGTCTTCACCGTCGTCCTGTC |
| mm3_intergenic_9230114K14Rik|Gm25601_chr5_52267759 |
TCTCTTCGGTCTCTCCTCTCCTTTCTTAGCTGGGACAAGTCCAAGTAAGCATTTTGCATGGAATGGTTGGGGCCATGCTT GGCCCAAGCCTCAAGTCATCTGGAGTTGGATTTCATAAAGGAAGAGTGAGAGGGGCA |
| mm4_intron_Lif_chr11_4264053 |
TCCTGGAGAGAAGCACTGGATACTGCACCTGGGCCTGGCATGGCAGCCCCTATCCCTGCTGAGGGATTCCCTGGGTGGAA ACGGGCTGTCCCCCTCATCCTGAAGCCTGTCAAACCCCTAGCAGG |
| mm4_intron_Ddx31_chr2_28896491 |
TCCCACTCCCCTCTTAACCCATGAAGCCCTGACTTAGAGCCTTTGGTGACATCCTGCTGTCACTTTCAAGACCTGGGCCT GGCATGGGCTCTCCCCCTCACCTCTCAAACCTGC |
| mm4_exon_Herpud2_chr9_25151466 |
GCAGAGCGAGCTTGAAGAGAGGACGGAGGCGGCCGGGGAGCAAGCCTGGGACAAGCAAGGCCGCGACCGAGCCTGAAGGA AAGAAGGCCGC |
| mm4_intergenic_Gm13358|Egfl7_chr2_26562674 |
AGCACCAGGAAAGCAGTGTTTCAGTCAGGAGCCACTTTGGGGTCAGCACTCGGGAGGAAAGGGCGAGAGGAGAGCCCATG GTTAGCCCAGGGGCATCATGAGATGGGGAGAG |
| mm4_exon_Amacr_chr15_10981835 |
AGATAAACTGTGGCTCGGGCGCCGAGCCGCTCCGCTCTCACCCTAAGGCTGGCCATGGTGCTGCGTGGCGTCAGGGTTGT GGAGCTGGCAGGCCTGGCCCCGGGGCCGTTCTGCGGAATGGTCC |
| mm4_intergenic_Pja1|Gm14816_chrX_99629645 |
GGGAGAAATCCAGGCAGCTTCCTAGAGACTTTGCTGAGCTAAAGAAGCAGCCACGCTGGGCCCTGGGGTCCTAGAGCGCT GCAGGTGCTGTTTGCCTTCTTGCAACTGTTTAGGGAGTCAGAGGC |
| mm3_intergenic_Arl14|Ppm1l_chr3_69283205 |
CCTGGGTGTGGTTGCTAGAATATTCTCAGACTGGTGAGGCCATGCTTGTCCCAAGCTACGAAGACTTAGCTGCTTTCTCC TGGAAACATTTACAAGCCAATTACTTATATTATGCAAATGCATCCCCTGAACTGCTAGG |
| mm3_intergenic_Ergic1|Gm22420_chr17_26576998 |
CATGCACCCCTGCTTGAGAGCTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTAGAGCAACGCTCTCTGAAGCTCAGCTT |
| mm4_intergenic_Gm15969|Casz1_chr4_148918563 |
CCAAGGCGAGGAGAAGGATGAGGAGAGGCCCTGCTAGCCCCAGGATGCTCTGGTTTTCTTGAGCTCCCTGCGGATCTTGG GCACAAATC |
| mm4_intergenic_Kcnb2|Gm25227_chr1_15401747 |
GCACAACATGGAGTGGAAGCTGTCCCAGCCTCCAGGCCCAGGCATGGGCGCTCTACACAGAAGAACACACTGTCTGAGTT GAAAATGGCAAAAAAAAGAAAAGACAAAGAAGCTGAAATGTTGAGTGAGCTGCAGTACA |
| mm2_intergenic_Fbxl5|Bst1_chr5_43782501 |
GGTTTTGCTAGGCCTGGGATGCTGGCTCTGGGCCTAGCATGGCCTCTCCCCTGCGGCTGAGAGCTGGGGACCTGGCAGCC CCTCCTCACCCCTCCTGGCACGGAGCAGAGTGGGATG |
| mm4_exon_Pde4a_chr9_21181798 | CTTCCGAGGCAGTGTACAGGACTGGGGCCGCCAGGCTTGGGCCGGGTGAGTGCGCACCTCTCAGGACACGGGTT |
| mm4_intron_Lrp1_chr10_127554518 |
AAGGAGACAACCATGGCCTGGCGGTGGGAAGAACTGGGGAGGCCATACTGGGCCCGGGCAGTCAACAACGGGTGTAAAGA AGGCTTGGTTTTGGCACCTGCTTAAGCAGTGTGTAGTTGGAGCCGT |
| mm4_intergenic_Gm9855|Foxo3_chr10_42108053 |
TCCAGGCCATAACCCTCTCAAGGGCTCCAGACAGGGGCGGGGCAACAGGTCATTGACCAGGACCCAGCATGACCTCTCCC CCACCTCATCCCTTAACCTCAAAAGAGCACTTCTGATGCAACCAAGG |
| mm4_intergenic_Gm23106|1700029M20Rik_chr4_135592187 |
TTGCTGGAACCAGGCAGTAGAACGAGCAGAGGAGTCTCAGAGCCTGGGCCCAGGATGGCAGCTCTGAGGTACCCCCCCAA TCACCTTTGGAGGGCGGTACCAAAGAGAC |
| mm4_intron_Dock9_chr14_121691512 |
CAGTCAGGACCGTGGGATGCTGAGCCCTGGGACAGGCCATGCTGAGCCCTGGGAGGCGAGGGGCAGCTCCTACCTCAGGT AACTGTCCAGACAGTGTACAGTACAAGTGTGCCTCCTTAGCACAGTGCCAG |
| mm4_intergenic_Ak8|Mir3088_chr2_28726440 |
TCTGGATCAGAATCAGGTGAGTTTAGAGTGTTGTACCAATAGAGGGGAGCAGACATGCTGGGCACAGGCAGTGGGGAAGG TGGCTGGTGTGTGGCCAGTGCCAGCAGAGGGATGAG |
| mm4_intron_Ncor2_chr5_125141445 |
GAGAAGCCCTCTCCCTCCTTGCTGCTGGCAGAGGGACCTGGGCCAGCCACGGCCCCTCCTTTAACCATTTCCTCCCAGCC AGCCAGCCCTGGCGTCCTCCAGTCCTGCAGGGCCAGATGGCCAGAGGAAAATGCTCCCGT |
| mm4_intergenic_Gtf2h1|Ldha_chr7_46835220 |
CGCCATCCCCTAGTGAGTTCCCCACTGAGCCCTGCATCCCCCAGTCCTGGGCCCTGCATCCCCGCTCCCTGGGGACCTTC ATGTCCCTGGGCCCTGCATCCCGCACCTCGCCCTGTAAGTTT |
| mm4_intron_Tax1bp1_chr6_52714053 |
CCGCGATTGTGACGTGTTTTGGGCTTGGGGTGACCGTAGAGTGGGAAGCGTGGCGGGAGCGGGCGTGCTAGGCCGCGGCT GGCCCCGGCTGGCCCCGGCGTCTAACAAGCCAGGGACGG |
| mm4_intron_Tiam1_chr16_89954674 |
CTGCTGGGCATGTCAGAGGGGAGTGGCCATGGCTGGCTCTGGATTCTGGCAGGGAGGAGCAACCTGCAATACTGGAAGAA CAGGTTGAAGCAGGGCCCACAACAGGACCAGGAATGCC |
| mm4_intergenic_Clybl|Gm5089_chr14_122406779 |
GGTGTAGCAGTGCTGGGGCGCAGCGGCCAGGCTAGGCCGGGGCCGCAGTGGGGGGATCCCCGTGGCCCAGTGGCCAGGAC TGCGGGCCTCCCGGACCCCGTGAGCCTGTCGCGCAGCCGGGCTGTAATGGTGCGAGAT |
| mm4_intergenic_Pex5l|AC113989.1_chr3_33217856 |
AGAACTGAGACTCACACGCGCATGCGACTTCCGGGCTCCCTCCGGAGCAGCCATGCTGGTTCCAGGGCTGTTCACAGATG CTCACACAGCCTTGGGGAAACACTGTGAGCGGGCACACCAAGTTCACCTCCCCTC |
| mm4_exon_1810013L24Rik_chr16_8830530 |
CCGGAGCATTGGTTCTCCAAGTGGGAGCGGCAGTGCCTGGCCGAGGCCGAGCAGGACGAGCAGTTGTCTCCCGAGCTGCA GGAGGAGGCGGCGGCCGCCGCGCAGCCCGAGCACAAGCAACAGAAGCTGTGGCATC |
| mm4_intron_Cdh13_chr8_118639810 |
GAGTCGTGAGTCTCAGGCAGGATTACCGAAGCCAGGCCAGGGCCCAGCATGGCCTGGCCTTAATGCTCTGCCTGGCAGTT CTCATGTTCATTACAACTGCTGGCTTCCTGACAGACAGTTCCCTTGTCTGGAGCCAA |
| mm4_intergenic_Mir9-3|AI854517_chr7_79505447 |
CCAGGGATTCCAGCTCGACGGAGCGGCCACGCCGGGACCAGGTATCCGACCTCGGCTCTCCTGGGGACACTTGCCGTGTC CTGGAGGCATTCCCTTCACCTC |
| mm4_intron_9930021J03Rik_chr19_29805122 |
GGCTACCTTCTCGGCTTTGGCGTGGGTGCCCACCACACCCCTCCGGTCCCGCGCCCAGCGCGGCCGCTCCTCCTCCCGCG GTTCTAGCACCAACTCA |
| mm4_intron_Pax5_chr4_44611415 | TGCTCCCTTGCACAGGTAGACCCGGCCCCAGCCTGGCCTCTCCCTTGGAGTTCTTGGGCCAGCTATGG |
| mm4_intron_Wdr19_chr5_65203836 |
ATCTTCGGCCGTTGGGAAATCACTGTAAATTCAGTGTAGCATAGGCACTCAGCAGTCACTAACGTGGAGACTCCCTGGCC TCAGAGGGCTGGTGCGGCCATGCTTCTCACTGGCTCGCTGCCCTCTGTGCTTTCCCTCAT |
| mm4_intron_Bag3_chr7_128531361 |
TCGTCAGGTCTGTAGGAGGGGGCTGGGAGCCAGCAGCAGCCAGACACTGCAGATCCGGAGCTGAGCAGACAGCGGCCGTG CGTGGCCCTGGCGAAGGAGTCTAATTATTTTTCTCTTGTGGCTGGCAGT |
| mm4_intergenic_Haghl|Narfl/mmu-mir-6966_chr17_25780172 |
TTAATCTCCTGGGCTGCAGCTGCCTGTTATCAACAGAGGTGGGAGAGACGATGCGTGGCCCTGGTCATTTGGTGGCCAGT GGGCATGAGTAACTACTTAAGTCACCTCTGCCTCACATCGCACACTTCTCAGCT |
| mm4_exon_Rab40b_chr11_121357153 |
CCTGACTTTCCGGAAGCTGTTCCTCTTGTGGCTGGAGTTGGCAGTGAGGGAGTAGGAGCGGCCATGCATCATCCTGGTGT TCAGGCCACTGGCCATCGAGAAAGACTTAAGGTGGCTTCTTAAGGCGAC |
| mm4_exon_Nsmf_chr2_25054451 |
CTCTCCTACAGTGGCTGGACGCGCTGGGCGCTGGGTGCGGCCATGCGGGGCCTGGGCCTAAGTCTGACCCTGACCCTGAG GCTGAGACCTAG |
| mm4_exon_Cd200_chr16_45400165 |
CCCACTGTTCCAAGGCTGATAGCATGCCCTTAAGTACCTCATGCAGATGAGTGGGCTAGCCTAGTAGGCTAAGGAGAGGC CATCCTTCACCCCGGAGGCTCTCCCGAGGAAGAACGGGGTATC |
| mm3_intergenic_Kif27|mmu-mir-6369/Kif27_chr13_58311514 |
TGCCTACAAAGCACTCTCTGTCTGTTAACTCAGGTTGGGAACCCCACCTGTGGAGAGGCCATGCTTCCCCCAGGATTCCA CTGGAGGCGCTCTCCAGCCTGCAAGGGCAGAGCTGGTCAGCTCCCTGTGACTA |