CRISPOR

DNA break repair outcome predictions

Guide: TCCCCCCCTCTGGAGAGTCC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGAT`	(Wild-type sequence)
519	`GTTTTCCCCCTCCCCCCCTCTGGAGAG---------------CCGAAAGGACATGGTTAT`	`15 bp deleted → no frameshift`
288	`GTTTTCCCCCTCCCCCCCTCTGGA-----------TGGAGAGCCGAAAGGACATGGTTAT`	`11 bp deleted → frameshift`
261	`GTTTTCCCCCTCCCCCCCTCTGGAGAG-------------AGCCGAAAGGACATGGTTAT`	`13 bp deleted → frameshift`
255	`GTTTTCCCCCTCCCCCCCTCTGG---------GGATGGAGAGCCGAAAGGACATGGTTAT`	`9 bp deleted → no frameshift`
242	`GTTTTCCCCCTCCCCCCCTCTGG----------GATGGAGAGCCGAAAGGACATGGTTAT`	`10 bp deleted → frameshift`
213	`GTTTTCCCCCTCCCCCCCTCTGGAG-----------------CCGAAAGGACATGGTTAT`	`17 bp deleted → frameshift`
198	`GTTTTCCCCCTCCCCCCCTCTGGAGAGTCC--------------GAAAGGACATGGTTAT`	`14 bp deleted → frameshift`
191	`GTTTTCCCCCTCCCCCCCTCTGGAGA---------TGGAGAGCCGAAAGGACATGGTTAT`	`9 bp deleted → no frameshift`
129	`GTTTTCCCCCTCCCCCCCTCTGGA---------------------------CATGGTTAT`	`27 bp deleted → no frameshift`
110	`GTTTTCCCCCTCCCCCCCTCTGGAGA--------------------AAGGACATGGTTAT`	`20 bp deleted → frameshift`
109	`GTTTTCCCCCTCCCCCCC--------------------------GAAAGGACATGGTTAT`	`26 bp deleted → frameshift`
103	`GTTTTCCCCCTCCCCCC---------------------------GAAAGGACATGGTTAT`	`27 bp deleted → no frameshift`
99	`GTTTTCCCCCTCCCCCCCTCTGGAGAG----------------------GACATGGTTAT`	`22 bp deleted → frameshift`
99	`GTTTTCCCCCTCCCCCCCTCTGGA----------------------AAGGACATGGTTAT`	`22 bp deleted → frameshift`
98	`GTTTTCCCCCTCCCCC----------------------------GAAAGGACATGGTTAT`	`28 bp deleted → frameshift`
96	`GTTTTCCCCCTCCCCCCCTCTGG---------------------------------TTAT`	`33 bp deleted → no frameshift`
94	`GTTTTCCCCCTCCCC-----------------------------GAAAGGACATGGTTAT`	`29 bp deleted → frameshift`
90	`GTTTTCCCCCTCCCCCCCTCTGGAG------------------------GACATGGTTAT`	`24 bp deleted → no frameshift`
89	`GTTTTCCCCCTCCC------------------------------GAAAGGACATGGTTAT`	`30 bp deleted → no frameshift`
86	`GTTTTCCCCCTCCCCCCCTCTGGAGA-------------------------CATGGTTAT`	`25 bp deleted → frameshift`
84	`GTTTTCCCCCTCC-------------------------------GAAAGGACATGGTTAT`	`31 bp deleted → frameshift`
73	`GTTTTCCCCC----------------------------------GAAAGGACATGGTTAT`	`34 bp deleted → frameshift`
70	`GTTTTCCCCCTCCCCCCCTCTGGAGAGT-----------------------------TAT`	`29 bp deleted → frameshift`
69	`GTTTTCCCC-----------------------------------GAAAGGACATGGTTAT`	`35 bp deleted → frameshift`
66	`GTTTTCCC------------------------------------GAAAGGACATGGTTAT`	`36 bp deleted → no frameshift`
62	`GTTTTCC-------------------------------------GAAAGGACATGGTTAT`	`37 bp deleted → frameshift`
25	`GTT-------------------------------------------------------AT`	`55 bp deleted → frameshift`
14	`GTTTT-----------------------------------------------------AT`	`53 bp deleted → frameshift`
13	`GTTT------------------------------------------------------AT`	`54 bp deleted → no frameshift`

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