CRISPOR

DNA break repair outcome predictions

Guide: ATCCCCGGACTCTCCAGAGG GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CCATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAG`	(Wild-type sequence)
469	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG--------GGGAAAACCCCCAAGTAAGCTC`	`8 bp deleted → frameshift`
362	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG--GGGAGGGGGAAAACCCCCAAGTAAGCTC`	`2 bp deleted → frameshift`
344	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG---GGAGGGGGAAAACCCCCAAGTAAGCTC`	`3 bp deleted → no frameshift`
327	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG----GAGGGGGAAAACCCCCAAGTAAGCTC`	`4 bp deleted → frameshift`
311	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG-----AGGGGGAAAACCCCCAAGTAAGCTC`	`5 bp deleted → frameshift`
255	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG---------GGAAAACCCCCAAGTAAGCTC`	`9 bp deleted → no frameshift`
242	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG----------GAAAACCCCCAAGTAAGCTC`	`10 bp deleted → frameshift`
230	`TCGGCTCTCCATCCCCGGACTCTCCAGAGG-----------AAAACCCCCAAGTAAGCTC`	`11 bp deleted → frameshift`
213	`TCGGCTCTCCATCCCCGGA-----------------GGGGGAAAACCCCCAAGTAAGCTC`	`17 bp deleted → frameshift`
198	`TCGGCTCTCCATCCCCGG--------------GGGAGGGGGAAAACCCCCAAGTAAGCTC`	`14 bp deleted → frameshift`
188	`TCGGCTCTCCATCCCCGG---------------GGAGGGGGAAAACCCCCAAGTAAGCTC`	`15 bp deleted → no frameshift`
182	`TCGGCTCTCCATCCCCGGACTCTCCAG----------GGGGAAAACCCCCAAGTAAGCTC`	`10 bp deleted → frameshift`
179	`TCGGCTCTCCATCCCCGG----------------GAGGGGGAAAACCCCCAAGTAAGCTC`	`16 bp deleted → frameshift`
158	`TCGGCTCTCCATCCCCGGA-----------------------AAACCCCCAAGTAAGCTC`	`23 bp deleted → frameshift`
153	`TCGGCTCTCCATCCCC---------------------------------CAAGTAAGCTC`	`33 bp deleted → no frameshift`
149	`TCGGCTCTCCATCCCCGGACTCTCCAGA--------------AAACCCCCAAGTAAGCTC`	`14 bp deleted → frameshift`
147	`TCGGCTCTCCATCCCCGG--------------------GGGAAAACCCCCAAGTAAGCTC`	`20 bp deleted → frameshift`
146	`TCGGCTCTCCATCCCC----------------------------------AAGTAAGCTC`	`34 bp deleted → frameshift`
143	`TCGGCTCTCCATCCCCGGACTCTCCA-------------------------AGTAAGCTC`	`25 bp deleted → frameshift`
140	`TCGGCTCTCCATCCCCGG---------------------GGAAAACCCCCAAGTAAGCTC`	`21 bp deleted → no frameshift`
133	`TCGGCTCTCCATCCCCGGACTCTCC----------------------CCCAAGTAAGCTC`	`22 bp deleted → frameshift`
133	`TCGGCTCTCCATCCCCGG----------------------GAAAACCCCCAAGTAAGCTC`	`22 bp deleted → frameshift`
126	`TCGGCTCTCCATCCCCGGACTCTCC-----------------------CCAAGTAAGCTC`	`23 bp deleted → frameshift`
121	`TCGGCTCTCCATCCCC--------------------------------CCAAGTAAGCTC`	`32 bp deleted → frameshift`
120	`TCGGCTCTCCATCCCCGGACTCTCC------------------------CAAGTAAGCTC`	`24 bp deleted → no frameshift`
104	`TCGGCTCTCCATCCC-----------------------------------AAGTAAGCTC`	`35 bp deleted → frameshift`
98	`TCGG----------------------------GGGAGGGGGAAAACCCCCAAGTAAGCTC`	`28 bp deleted → frameshift`
94	`TCGG-----------------------------GGAGGGGGAAAACCCCCAAGTAAGCTC`	`29 bp deleted → frameshift`
90	`TCGGCTCTCCATCCCCGGACTCTCCAGAG------------------------TAAGCTC`	`24 bp deleted → no frameshift`
89	`TCGG------------------------------GAGGGGGAAAACCCCCAAGTAAGCTC`	`30 bp deleted → no frameshift`
84	`TCGGCTCTCCATCCCC-------------------------------CCCAAGTAAGCTC`	`31 bp deleted → frameshift`
84	`TCGG-------------------------------AGGGGGAAAACCCCCAAGTAAGCTC`	`31 bp deleted → frameshift`
82	`TCGGCTCTCCATCCCCGGACTCTC------------------------------------`	`36 bp deleted → no frameshift`
81	`TCGGCTCTCCATCCCCGGACTCTCCAG--------------------------TAAGCTC`	`26 bp deleted → frameshift`
81	`TCGGCTCTCCATCCCCGGAC--------------------------CCCCAAGTAAGCTC`	`26 bp deleted → frameshift`
75	`TCGGCTCTCCATCCCCGGACTC--------------------------------------`	`38 bp deleted → frameshift`
74	`TCGGCTCTCCATCCCCGGACTCTCCAGAG----------------------------CTC`	`28 bp deleted → frameshift`
73	`TCGG----------------------------------GGGAAAACCCCCAAGTAAGCTC`	`34 bp deleted → frameshift`
69	`TCGG-----------------------------------GGAAAACCCCCAAGTAAGCTC`	`35 bp deleted → frameshift`
67	`TCGGCTCTCCA----------------------------------------AGTAAGCTC`	`40 bp deleted → frameshift`
66	`TCGGCTCTCCATCCCCGGACTCTCCAG------------------------------CTC`	`30 bp deleted → no frameshift`
66	`TCGGCTCTCCATCC------------------------------------AAGTAAGCTC`	`36 bp deleted → no frameshift`
66	`TCGG------------------------------------GAAAACCCCCAAGTAAGCTC`	`36 bp deleted → no frameshift`
62	`TCGGCTCTCC-------------------------------------CCCAAGTAAGCTC`	`37 bp deleted → frameshift`
62	`TCGG-------------------------------------AAAACCCCCAAGTAAGCTC`	`37 bp deleted → frameshift`
60	`TCGGCTCTCC--------------------------------------CCAAGTAAGCTC`	`38 bp deleted → frameshift`
56	`TCGGCTCTCC---------------------------------------CAAGTAAGCTC`	`39 bp deleted → no frameshift`
49	`TCGGCTC-----------------------------------------------------`	`53 bp deleted → frameshift`
39	`TCGGCTCTC---------------------------------------------------`	`51 bp deleted → no frameshift`
28	`TCGGCTCTCCATC-----------------------------------------------`	`47 bp deleted → frameshift`
16	`TC----------------------------------------------------------`	`58 bp deleted → frameshift`

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