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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314099_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314099_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm4_intergenic_Gm7327|Gm6743_chrX_61798411_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_Gm7327|Gm6743_chrX_61798411_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm2_intergenic_Antxrl|Anxa8_chr14_34079232_F | TCGTCGGCAGCGTCACCCACCTTTCACGACATCC | 59.9 | 0.50 |
| mm2_intergenic_Antxrl|Anxa8_chr14_34079232_R | GTCTCGTGGGCTCGGGCACACAAGACCCCTTCTGT | 60.1 | 0.50 |
| mm4_intergenic_Tspyl5|Mtdh_chr15_33992970_F | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_Tspyl5|Mtdh_chr15_33992970_R | Primer3: not found at this Tm | N.d. | 0.41 |
| mm4_intergenic_RP24-86O15.2|Gm10698_chr9_33167630_F | TCGTCGGCAGCGTCAACACGCAAGCCCTGTAGAA | 59.8 | 0.39 |
| mm4_intergenic_RP24-86O15.2|Gm10698_chr9_33167630_R | GTCTCGTGGGCTCGGTAGAAGCTGTCCTGAGGCAC | 59.1 | 0.39 |
| mm4_intron_Trpm3_chr19_22984594_F | TCGTCGGCAGCGTCGAGCAGTAGGAAGACTGGGC | 59.8 | 0.36 |
| mm4_intron_Trpm3_chr19_22984594_R | GTCTCGTGGGCTCGGTCCAAAACGAACAGGAGCCA | 59.8 | 0.36 |
| mm4_intergenic_Gm22246|Flrt2_chr12_95621455_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Gm22246|Flrt2_chr12_95621455_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm3_intron_Gm12602_chr4_89115602_F | TCGTCGGCAGCGTCAGAATCACCACTCAGCTGGG | 59.3 | 0.33 |
| mm3_intron_Gm12602_chr4_89115602_R | GTCTCGTGGGCTCGGATCCCACAGAAATGCCTGGG | 60.0 | 0.33 |
| mm4_intergenic_Foxm1/4933413G19Rik|4933413G19Rik_chr6_128379382_F | TCGTCGGCAGCGTCGCACCTCTGCCATATCCTGT | 59.5 | 0.33 |
| mm4_intergenic_Foxm1/4933413G19Rik|4933413G19Rik_chr6_128379382_R | GTCTCGTGGGCTCGGCACGCTGAGTCCAAGTCTGA | 59.6 | 0.33 |
| mm4_intergenic_Gm5742|Gm22223_chr8_100922418_F | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intergenic_Gm5742|Gm22223_chr8_100922418_R | Primer3: not found at this Tm | N.d. | 0.32 |
| mm4_intron_Net1_chr13_3905785_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Net1_chr13_3905785_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97883635_F | TCGTCGGCAGCGTCAATCTCCAAGCCTCAGCCAC | 60.0 | 0.30 |
| mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97883635_R | GTCTCGTGGGCTCGGACGACTCCATCCCTCACTGA | 59.9 | 0.30 |
| mm4_intron_Acsbg1_chr9_54616162_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Acsbg1_chr9_54616162_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Ak4_chr4_101449087_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Ak4_chr4_101449087_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Jdp2|Batf_chr12_85675528_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Jdp2|Batf_chr12_85675528_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intergenic_Gm24478|Gm14255_chr2_162856322_F | TCGTCGGCAGCGTCCCTTGTCACATGGCAGCTTG | 59.7 | 0.24 |
| mm4_intergenic_Gm24478|Gm14255_chr2_162856322_R | GTCTCGTGGGCTCGGCTAGAGGGTGGTTGTGGCTG | 60.0 | 0.24 |
| mm4_intron_Coro1c_chr5_113845828_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Coro1c_chr5_113845828_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Ppm1f_chr16_16912857_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Ppm1f_chr16_16912857_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm3_intron_Mn1_chr5_111451459_F | TCGTCGGCAGCGTCTTCTGCTGTGGGAATCTGGC | 60.3 | 0.21 |
| mm3_intron_Mn1_chr5_111451459_R | GTCTCGTGGGCTCGGACACCTAGGCTGCTGGACTA | 59.9 | 0.21 |
| mm4_intergenic_Gemin8|Gm15227_chrX_166199961_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gemin8|Gm15227_chrX_166199961_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Golim4|Fstl5_chr3_76065233_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Golim4|Fstl5_chr3_76065233_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Hk2_chr6_82751354_F | TCGTCGGCAGCGTCGACCATGAGGAACGGAGGTG | 60.1 | 0.18 |
| mm4_intron_Hk2_chr6_82751354_R | GTCTCGTGGGCTCGGCCACCAGAAAGTACAGCCGA | 59.6 | 0.18 |
| mm4_intron_Atf7ip_chr6_136591441_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Atf7ip_chr6_136591441_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Anp32b|Nans_chr4_46487272_F | TCGTCGGCAGCGTCAAAGAAACGCTAGAATAACTGCTC | 57.5 | 0.17 |
| mm4_intergenic_Anp32b|Nans_chr4_46487272_R | GTCTCGTGGGCTCGGAAACTCAGTCCCTTTTGGCT | 57.2 | 0.17 |
| mm4_exon_Slc35e2_chr4_155601617_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Slc35e2_chr4_155601617_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_E2f7_chr10_110749875_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_E2f7_chr10_110749875_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_BC002163|Zbtb20_chr16_43002940_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_BC002163|Zbtb20_chr16_43002940_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Morc1|Trat1_chr16_48719155_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Morc1|Trat1_chr16_48719155_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gdap1|Gm25166_chr1_17279765_F | TCGTCGGCAGCGTCACAGCTCCAAAATGGGAAGA | 57.0 | 0.15 |
| mm4_intergenic_Gdap1|Gm25166_chr1_17279765_R | GTCTCGTGGGCTCGGGCCCTCCAAACCATCTTCCT | 59.6 | 0.15 |
| mm4_intergenic_Klhl14|Klhl14/4930426D05Rik_chr18_21636470_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Klhl14|Klhl14/4930426D05Rik_chr18_21636470_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm3_intron_Htra1_chr7_130971242_F | TCGTCGGCAGCGTCTTGAGCATTTGCCATGACGG | 59.4 | 0.14 |
| mm3_intron_Htra1_chr7_130971242_R | GTCTCGTGGGCTCGGGGGAGCCAACAGCCATACAT | 60.1 | 0.14 |
| mm3_intron_Gata2_chr6_88200925_F | TCGTCGGCAGCGTCGGTGTGGTGTGCCTCTGTAA | 59.8 | 0.14 |
| mm3_intron_Gata2_chr6_88200925_R | GTCTCGTGGGCTCGGGGTTTCTTCCTCCCCACAGG | 59.9 | 0.14 |
| mm4_exon_D030018L15Rik_chr15_96075447_F | TCGTCGGCAGCGTCCTTCAGCAGAGACCGATGCA | 60.1 | 0.13 |
| mm4_exon_D030018L15Rik_chr15_96075447_R | GTCTCGTGGGCTCGGGATTCCTCATGGCTGCTGGT | 60.1 | 0.13 |
| mm4_intron_Tgtp2_chr11_49063719_F | TCGTCGGCAGCGTCGGACTCCAAGGGCATCTGAG | 59.8 | 0.13 |
| mm4_intron_Tgtp2_chr11_49063719_R | GTCTCGTGGGCTCGGAACCCCTACCTTCCACCTGT | 60.1 | 0.13 |
| mm4_intron_Gm12185_chr11_48926687_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Gm12185_chr11_48926687_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Tgtp1|Tgtp1/Gm12185_chr11_48991760_F | TCGTCGGCAGCGTCAGCACAAACTCCAAGGGCAT | 60.1 | 0.13 |
| mm4_intergenic_Tgtp1|Tgtp1/Gm12185_chr11_48991760_R | GTCTCGTGGGCTCGGAACCCCTACCTTCCACCTGT | 60.1 | 0.13 |
| mm4_intergenic_Ifi47|Ifi47/Olfr56_chr11_49080789_F | TCGTCGGCAGCGTCGGACTCCAAGGGCATCTGAG | 59.8 | 0.13 |
| mm4_intergenic_Ifi47|Ifi47/Olfr56_chr11_49080789_R | GTCTCGTGGGCTCGGGAAGAGGAGGCAACCCCTTC | 60.0 | 0.13 |
| mm4_intergenic_Ssu2|Cav3_chr6_112449167_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Ssu2|Cav3_chr6_112449167_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gm23796|Gm23831_chr3_39779737_F | TCGTCGGCAGCGTCTCTTTCCATTGTGCTCTGCT | 57.4 | 0.12 |
| mm4_intergenic_Gm23796|Gm23831_chr3_39779737_R | GTCTCGTGGGCTCGGGCAGACCACCAAATAATCGCA | 59.2 | 0.12 |
| mm4_intergenic_Hist1h2al|S1pr3_chr13_51352206_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Hist1h2al|S1pr3_chr13_51352206_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_A430105I19Rik|Phgr1_chr2_118763132_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_A430105I19Rik|Phgr1_chr2_118763132_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm25682|Adgb_chr10_10256165_F | TCGTCGGCAGCGTCCCCAAAGACAAGCATCGCTG | 59.8 | 0.11 |
| mm4_intergenic_Gm25682|Adgb_chr10_10256165_R | GTCTCGTGGGCTCGGACTGTGAGCCTGAAGGTACAC | 59.6 | 0.11 |
| mm4_intron_Slc22a19_chr19_7684411_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Slc22a19_chr19_7684411_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gstp2|BC021614_chr19_4056381_F | TCGTCGGCAGCGTCAGCCTCGGGACACTCTCTAG | 60.1 | 0.11 |
| mm4_intergenic_Gstp2|BC021614_chr19_4056381_R | GTCTCGTGGGCTCGGGGCAGGAAGTCTGGGAGTTT | 59.5 | 0.11 |
| mm4_intron_Plce1_chr19_38731748_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Plce1_chr19_38731748_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rpp40|Ppp1r3g_chr13_35944390_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Rpp40|Ppp1r3g_chr13_35944390_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Paip2b_chr6_83815316_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Paip2b_chr6_83815316_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Jph2_chr2_163355890_F | TCGTCGGCAGCGTCCGAGCACTCAGGGGAAGATG | 60.1 | 0.07 |
| mm4_intron_Jph2_chr2_163355890_R | GTCTCGTGGGCTCGGAACCTCTGTGCTCAGCCATC | 60.0 | 0.07 |
| mm4_intergenic_Grtp1|Adprhl1_chr8_13213725_F | TCGTCGGCAGCGTCCTGACAGAGGGCAGGAAGTG | 60.0 | 0.07 |
| mm4_intergenic_Grtp1|Adprhl1_chr8_13213725_R | GTCTCGTGGGCTCGGGAACATACTTCCGGCCCCTG | 60.4 | 0.07 |
| mm4_intergenic_Nsmf|Entpd8_chr2_25066410_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Nsmf|Entpd8_chr2_25066410_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Atxn2|Gm16552_chr5_121715853_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Atxn2|Gm16552_chr5_121715853_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Atp1b3_chr9_96333452_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Atp1b3_chr9_96333452_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Acap2_chr16_31172918_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Acap2_chr16_31172918_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tmem132e_chr11_82403626_F | TCGTCGGCAGCGTCGCCATAAACCTCAGCCAGCT | 60.3 | 0.07 |
| mm4_intron_Tmem132e_chr11_82403626_R | GTCTCGTGGGCTCGGGACTAGTGAGCCATGCCTGG | 60.1 | 0.07 |
| mm4_intron_Pbx3_chr2_34174796_F | TCGTCGGCAGCGTCTCCCATCTGAAGTAAACATCATTCC | 58.8 | 0.07 |
| mm4_intron_Pbx3_chr2_34174796_R | GTCTCGTGGGCTCGGAAGAGTGCTCGTTGACAACC | 58.4 | 0.07 |
| mm3_intron_Smarca4_chr9_21678784_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Smarca4_chr9_21678784_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Itgb2l|Pcp4_chr16_96452273_F | TCGTCGGCAGCGTCCCTGAGGAGCAAGTGTCTGC | 60.6 | 0.06 |
| mm4_intergenic_Itgb2l|Pcp4_chr16_96452273_R | GTCTCGTGGGCTCGGTTCTCATGCCTGTTGGGAGC | 60.3 | 0.06 |
| mm4_intergenic_Gm25248|Gm26326_chr12_43479952_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm25248|Gm26326_chr12_43479952_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Phtf1_chr3_103973692_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Phtf1_chr3_103973692_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Kcnh8_chr17_52766480_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Kcnh8_chr17_52766480_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Grik3|2610028E06Rik_chr4_125716512_F | TCGTCGGCAGCGTCTATGCAAATGAGGCCGCTGA | 60.1 | 0.05 |
| mm4_intergenic_Grik3|2610028E06Rik_chr4_125716512_R | GTCTCGTGGGCTCGGTGGATCCTGTGTAGGGCTGA | 59.9 | 0.05 |
| mm4_intron_Nxn_chr11_76296154_F | TCGTCGGCAGCGTCCCCGACTTGCCAAGAAAAGC | 60.0 | 0.05 |
| mm4_intron_Nxn_chr11_76296154_R | GTCTCGTGGGCTCGGCTCCTCCCTCTGCTTTGGTG | 60.0 | 0.05 |
| mm4_intron_Spag1_chr15_36184515_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Spag1_chr15_36184515_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm14454|Gm14642_chr2_173340977_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm14454|Gm14642_chr2_173340977_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_4930548K13Rik_chr4_26704077_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_4930548K13Rik_chr4_26704077_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Gramd1b|1700110K17Rik_chr9_40321603_F | TCGTCGGCAGCGTCCTGGCCAATGTCCTGCAGTA | 60.0 | 0.05 |
| mm3_intergenic_Gramd1b|1700110K17Rik_chr9_40321603_R | GTCTCGTGGGCTCGGGCCAGCAACTTTCCATTGCA | 59.9 | 0.05 |
| mm4_intron_Tenm3_chr8_48267610_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Tenm3_chr8_48267610_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Zfp423_chr8_87738186_F | TCGTCGGCAGCGTCTAACTGTGGTAGCCAGCCAG | 59.3 | 0.04 |
| mm4_intron_Zfp423_chr8_87738186_R | GTCTCGTGGGCTCGGGGAGGACACAGGATGGGAAC | 59.7 | 0.04 |
| mm4_intergenic_Ggact|Pcca_chr14_122859164_F | TCGTCGGCAGCGTCACGAAGGGTAGTCATTCTTGTAA | 57.2 | 0.04 |
| mm4_intergenic_Ggact|Pcca_chr14_122859164_R | GTCTCGTGGGCTCGGTGTGTCCTCTCTTTAAGTGATGTGT | 59.9 | 0.04 |
| mm4_intron_9530036O11Rik_chr5_28827690_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_9530036O11Rik_chr5_28827690_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Nmnat2_chr1_153071218_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Nmnat2_chr1_153071218_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Pard3_chr8_127144807_F | TCGTCGGCAGCGTCTGAGTCCTCAGGAAGTGGGT | 59.8 | 0.04 |
| mm4_intron_Pard3_chr8_127144807_R | GTCTCGTGGGCTCGGCCCGGCAGACTCTTAAGGTG | 60.1 | 0.04 |
| mm4_intergenic_Abhd6|Rpp14_chr14_8063029_F | TCGTCGGCAGCGTCCCACTCCCTCCACTGTCAAC | 59.9 | 0.04 |
| mm4_intergenic_Abhd6|Rpp14_chr14_8063029_R | GTCTCGTGGGCTCGGAAACAGGGTGCAAGGGACC | 60.1 | 0.04 |
| mm4_intron_Ubr2_chr17_46960816_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ubr2_chr17_46960816_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Ccnd1|Tpcn2_chr7_145099977_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_Ccnd1|Tpcn2_chr7_145099977_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intron_Erc2_chr14_28088633_F | TCGTCGGCAGCGTCAGACCTGAGGCTCACACAGA | 60.1 | 0.03 |
| mm3_intron_Erc2_chr14_28088633_R | GTCTCGTGGGCTCGGGCAAATGTCTGCTTACCCCAC | 59.7 | 0.03 |
| mm4_intergenic_Gm13323|H2afb1_chr2_17943644_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm13323|H2afb1_chr2_17943644_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Hdac4_chr1_92000817_F | TCGTCGGCAGCGTCGAACCCCTCCATGGCAGAAG | 60.3 | 0.03 |
| mm4_intron_Hdac4_chr1_92000817_R | GTCTCGTGGGCTCGGGAGGGTGAAATAGGTGGCCC | 60.1 | 0.03 |
| mm4_intron_Ppef2_chr5_92238170_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Ppef2_chr5_92238170_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_RP24-390G17.1|Afap1l1/RP24-390G17.1_chr18_61734628_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_RP24-390G17.1|Afap1l1/RP24-390G17.1_chr18_61734628_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Atp2a2|Gm22965_chr5_122503042_F | TCGTCGGCAGCGTCCACACAGCCTTTGACACACG | 59.9 | 0.02 |
| mm4_intergenic_Atp2a2|Gm22965_chr5_122503042_R | GTCTCGTGGGCTCGGAGGCTCCTTCTCTCGCATTG | 59.8 | 0.02 |
| mm4_intergenic_Gm12122|Slit3_chr11_35493432_F | TCGTCGGCAGCGTCGCCCTTGTGGACTGTCCTAC | 60.0 | 0.02 |
| mm4_intergenic_Gm12122|Slit3_chr11_35493432_R | GTCTCGTGGGCTCGGGCTAGATCAAGCCAGCTCCC | 60.2 | 0.02 |
| mm4_intergenic_Gm23437|Gm24818_chr14_108031973_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23437|Gm24818_chr14_108031973_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Eya1|Msc_chr1_14416920_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Eya1|Msc_chr1_14416920_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm24240|Rpl21-ps7_chr17_83287492_F | TCGTCGGCAGCGTCAGCCTGCAGATCAGCTCATC | 59.8 | 0.02 |
| mm4_intergenic_Gm24240|Rpl21-ps7_chr17_83287492_R | GTCTCGTGGGCTCGGAGGAAGACATGGCCTCCAGA | 60.2 | 0.02 |
| mm4_intergenic_Bmp7|Spo11_chr2_172970058_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Bmp7|Spo11_chr2_172970058_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Pdxk_chr10_78463955_F | TCGTCGGCAGCGTCTGCTCAGTGACCAGCCAAAA | 60.1 | 0.02 |
| mm4_intron_Pdxk_chr10_78463955_R | GTCTCGTGGGCTCGGGCAATATCAGCTGACTGGAGC | 59.1 | 0.02 |
| mm4_intron_Pdxk-ps_chr17_32099386_F | TCGTCGGCAGCGTCAAGGTACATTGCAGGAGGCC | 60.0 | 0.02 |
| mm4_intron_Pdxk-ps_chr17_32099386_R | GTCTCGTGGGCTCGGCAGAGGCAATGTCAGGCTGA | 60.0 | 0.02 |
| mm4_intergenic_Gm17276|Notch3_chr17_32114804_F | TCGTCGGCAGCGTCCAGAGGCAATGTCAGGCTGA | 60.0 | 0.02 |
| mm4_intergenic_Gm17276|Notch3_chr17_32114804_R | GTCTCGTGGGCTCGGAAGGTACATTGCAGGAGGCC | 60.0 | 0.02 |
| mm4_intergenic_Lef1|Hadh_chr3_131228947_F | TCGTCGGCAGCGTCCCCCTGACCTCCACATTTGG | 60.3 | 0.02 |
| mm4_intergenic_Lef1|Hadh_chr3_131228947_R | GTCTCGTGGGCTCGGGTCTTGGGTGAGCACAGCTA | 59.6 | 0.02 |
| mm3_intergenic_2310007B03Rik|E030010N08Rik_chr1_93182610_F | TCGTCGGCAGCGTCAGACCCAGCCCCGAATTATG | 59.5 | 0.02 |
| mm3_intergenic_2310007B03Rik|E030010N08Rik_chr1_93182610_R | GTCTCGTGGGCTCGGTGTGACAGTTGGGGTCAAGG | 59.8 | 0.02 |
| mm4_intron_Ccdc67_chr9_15573241_F | TCGTCGGCAGCGTCCACAGTATGCCCACCACGG | 60.7 | 0.01 |
| mm4_intron_Ccdc67_chr9_15573241_R | GTCTCGTGGGCTCGGCATACTGTGCAGGAGGGGC | 60.1 | 0.01 |
| mm4_intron_Ccdc67_chr9_15573213_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ccdc67_chr9_15573213_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Xlr_chrX_53778302_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Xlr_chrX_53778302_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm23831|1700017G19Rik_chr3_40344555_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm23831|1700017G19Rik_chr3_40344555_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm11185|Ttc39b_chr4_83202692_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm11185|Ttc39b_chr4_83202692_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm7589|Adpgk_chr9_59260037_F | TCGTCGGCAGCGTCTGAGAGGGAGGGAACAAAGC | 59.3 | 0.01 |
| mm4_intergenic_Gm7589|Adpgk_chr9_59260037_R | GTCTCGTGGGCTCGGGGCTTTTCCCACTGGCTGTA | 60.2 | 0.01 |
| mm4_intergenic_Fbxl14|Wnt5b_chr6_119522367_F | TCGTCGGCAGCGTCCCCTCCTCAAAGGCTGACTG | 60.0 | 0.01 |
| mm4_intergenic_Fbxl14|Wnt5b_chr6_119522367_R | GTCTCGTGGGCTCGGTTTATCAGCATGCCCAACGC | 59.5 | 0.01 |
| mm4_intergenic_Rilpl1|Tmed2_chr5_124532637_F | TCGTCGGCAGCGTCGAGAATGGCTGCATCCGGTA | 59.8 | 0.01 |
| mm4_intergenic_Rilpl1|Tmed2_chr5_124532637_R | GTCTCGTGGGCTCGGCCATAGTGCAACAGCTCCCA | 60.0 | 0.01 |
| mm4_intron_Lsamp_chr16_41777769_F | TCGTCGGCAGCGTCGTCAAGAGTGCATGGAGGCA | 60.3 | 0.01 |
| mm4_intron_Lsamp_chr16_41777769_R | GTCTCGTGGGCTCGGAGATGGTTGCCTTGCTCCAA | 59.8 | 0.01 |
| mm4_intergenic_Dhx32|Fank1_chr7_133791117_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Dhx32|Fank1_chr7_133791117_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cspp1_chr1_10080958_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cspp1_chr1_10080958_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_RP24-225I21.1|Gm26662_chr9_34551380_F | TCGTCGGCAGCGTCGCACCAGCTGACAGGTTCTA | 59.6 | 0.00 |
| mm4_intergenic_RP24-225I21.1|Gm26662_chr9_34551380_R | GTCTCGTGGGCTCGGACAATTGAAGCAACCAAGCTCA | 59.3 | 0.00 |
| mm3_intergenic_Lrrtm4|Gm26264_chr6_80879845_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intergenic_Lrrtm4|Gm26264_chr6_80879845_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lrp1b_chr2_41622570_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lrp1b_chr2_41622570_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sipa1l1_chr12_82367963_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Sipa1l1_chr12_82367963_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_St6galnac2|Gm11735_chr11_116705440_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_St6galnac2|Gm11735_chr11_116705440_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gapvd1_chr2_34678259_F | TCGTCGGCAGCGTCTTACTCGGTGCTGTGCTCTG | 60.0 | 0.00 |
| mm4_intron_Gapvd1_chr2_34678259_R | GTCTCGTGGGCTCGGTCGGGATTGTTGTCCAGAGC | 60.0 | 0.00 |
| mm4_intron_Ubxn11_chr4_134119394_F | TCGTCGGCAGCGTCTGATTTGCCCTGGCCTAGTC | 59.7 | 0.00 |
| mm4_intron_Ubxn11_chr4_134119394_R | GTCTCGTGGGCTCGGTTCCCATGAGACACTGGCAC | 59.9 | 0.00 |
| mm4_intron_Ttc28_chr5_111210710_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ttc28_chr5_111210710_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Banf1_chr19_5366432_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Banf1_chr19_5366432_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22016|Cdc73_chr1_142475403_F | TCGTCGGCAGCGTCTGGCATACTGGAGTTCCCAT | 58.4 | 0.00 |
| mm4_intergenic_Gm22016|Cdc73_chr1_142475403_R | GTCTCGTGGGCTCGGACTGGAATAGAAAGGCCTCCTC | 59.2 | 0.00 |
| mm4_intron_Syt1_chr10_108600781_F | TCGTCGGCAGCGTCATGCAAGCCGTTCTCCTCAA | 59.9 | 0.00 |
| mm4_intron_Syt1_chr10_108600781_R | GTCTCGTGGGCTCGGAATGAAACACTCCCTGCCAT | 57.3 | 0.00 |
| mm4_intron_Slc46a3_chr5_147884045_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Slc46a3_chr5_147884045_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ptprm_chr17_67090781_F | TCGTCGGCAGCGTCCCTAAATCCAGGGCAGCAGG | 60.4 | 0.00 |
| mm4_intron_Ptprm_chr17_67090781_R | GTCTCGTGGGCTCGGTGGCCTGATTGTTGCTGAAC | 59.0 | 0.00 |
| mm4_intron_Cux1_chr5_136500806_F | TCGTCGGCAGCGTCTTCACAGGTACAGCCAGCTG | 59.9 | 0.00 |
| mm4_intron_Cux1_chr5_136500806_R | GTCTCGTGGGCTCGGAAGCTCTGTGGAAGTGGTGG | 59.8 | 0.00 |
| mm4_intron_Bend7_chr2_4736344_F | TCGTCGGCAGCGTCTATGCTGTGTGGAGCCCTTG | 60.0 | 0.00 |
| mm4_intron_Bend7_chr2_4736344_R | GTCTCGTGGGCTCGGTGCAGTCTTCAGTGAAGCCC | 60.2 | 0.00 |
| mm4_intergenic_Rnf165|8030462N17Rik_chr18_77604625_F | TCGTCGGCAGCGTCACCATTTCAGCAGGTCCTCG | 60.0 | 0.00 |
| mm4_intergenic_Rnf165|8030462N17Rik_chr18_77604625_R | GTCTCGTGGGCTCGGGGAGCCACCTAAGGGTATGC | 59.8 | 0.00 |
| mm4_intergenic_Ptpn3|Gm12536/Ptpn3_chr4_57282819_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ptpn3|Gm12536/Ptpn3_chr4_57282819_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pomgnt2|Snrk_chr9_122023997_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Pomgnt2|Snrk_chr9_122023997_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23562|4930548G14Rik_chr15_46521604_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23562|4930548G14Rik_chr15_46521604_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12804|Gm12805_chr4_111310953_F | TCGTCGGCAGCGTCCTTCTACCTTCCAGCCAGGC | 60.1 | 0.00 |
| mm4_intergenic_Gm12804|Gm12805_chr4_111310953_R | GTCTCGTGGGCTCGGAGGATTCTCATGAAGGGATGAACA | 59.5 | 0.00 |
| mm4_exon_Grik3_chr4_125709656_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Grik3_chr4_125709656_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Bcas1_chr2_170347482_F | TCGTCGGCAGCGTCTCCCTGTGCCCCAGAAGTAA | 60.4 | 0.00 |
| mm4_exon_Bcas1_chr2_170347482_R | GTCTCGTGGGCTCGGGGGCTGTAAAGAAGAATGGGC | 59.2 | 0.00 |
| mm3_exon_Heatr5a_chr12_51947800_F | TCGTCGGCAGCGTCACCTTGCCCTTTCCATGAGG | 59.9 | 0.00 |
| mm3_exon_Heatr5a_chr12_51947800_R | GTCTCGTGGGCTCGGACGGCTCGCAATACTCAAGT | 59.7 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314099 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm2_intergenic_Antxrl|Anxa8_chr14_34079232 |
ACCCACCTTTCACGACATCCCTGGGAAGCTGTGAGCTGTGCTGGCGGAAGAGAGCCCATCACCATGTCCCCACCCAAGCA TGGCCTCCCCAGCAGAGTCCCACACCCGCCTCCAGCCTACAGAAGGGGTCTTGTGTGC |
| mm4_intergenic_RP24-86O15.2|Gm10698_chr9_33167630 |
AACACGCAAGCCCTGTAGAATGGGAGGCNNNNNNNNNNNNNNNNNNNNNGCCCTTTTAATCATGGGAGCTGTTGGAGCAG CCATACTGGGGTGCCTCAGGACAGCTTCTA |
| mm4_intron_Trpm3_chr19_22984594 |
GAGCAGTAGGAAGACTGGGCCTAAAGACCCAGCATGGCCTCTTAAGCAGGCCCAGGGCTGCAAACTCAGGCCTTGTTCTT CTTCTCTGGCTCCTGTTCGTTTTGGA |
| mm3_intron_Gm12602_chr4_89115602 |
AGAATCACCACTCAGCTGGGCCTGAACCTCAGTCAGGAGCCCCAGCATGACGGCTCCAGCAGGGCTTCCCCAGGCATTTC TGTGGGAT |
| mm4_intergenic_Foxm1/4933413G19Rik|4933413G19Rik_chr6_128379382 |
GCACCTCTGCCATATCCTGTTCTCTGCTGGCACAGCCATTCTAGGCTCCCTTTCATGTCTCCCTAGATGATCCATGCCAA AGAATCCTCCCCAATCTATCAGACTTGGACTCAGCGTG |
| mm4_intergenic_Gm26619/Gm10260|Arhgef28_chr13_97883635 |
AATCTCCAAGCCTCAGCCACTCTGTAAGCCAAGGATGACTGTTCCAGCAGAGTCGAATGTGAGGTACGATCACGACACAG GGTCCCTGTGGGTCAGTGAGGGATGGAGTCGT |
| mm4_intergenic_Gm24478|Gm14255_chr2_162856322 |
CCTTGTCACATGGCAGCTTGCTGTTACTCCACAGCTGACTGCTGTGGGAAAATGACCTTCCCAGCATGGGCTCTCCAGTA GGCTTCCTCACCCTTGAACTGGAATTCTACCAGCTCATCCAGCCACAACCACCCTCTAG |
| mm3_intron_Mn1_chr5_111451459 |
TTCTGCTGTGGGAATCTGGCTCTCCTAGCATGGCCCCTCCAGTAGCAAATGGAGAAACAGGTACTGCAGATAGTCCAGCA GCCTAGGTGT |
| mm4_intron_Hk2_chr6_82751354 |
GACCATGAGGAACGGAGGTGGCCGGCACTGCCTCTGGCTGGTTATCATCCAAGACCAGCCCAGCTGGACTCGGCCCAGCA TGGTCGGTCCATCAGGAATGTGGTTCGGCTGTACTTTCTGGTGG |
| mm4_intergenic_Anp32b|Nans_chr4_46487272 |
AAAGAAACGCTAGAATAACTGCTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNCGACATTGTTTTCTTTAGCCAAAAGGGACTGAGTTT |
| mm4_intergenic_Gdap1|Gm25166_chr1_17279765 |
ACAGCTCCAAAATGGGAAGAAAACAGCATTTCTGTCCTTGAGAGGCCATGCTTGGTTAGAGGCCCTAACAATAACATGTG AGTATAACTGTTGATACAGAAAGGCATTCTGATGAGGAAGATGGTTTGGAGGGC |
| mm3_intron_Htra1_chr7_130971242 |
TTGAGCATTTGCCATGACGGGACACTGCTGCAGCAGCCATGCTGAGGTCGGTGGTCAAACGGGTTGATGTAGCTGGATTT GATCAACATCCTGTTTCTTCTCCTGTGGGTGCCACAGAATATGTATGGCTGTTGGCTCCC |
| mm3_intron_Gata2_chr6_88200925 |
GGTGTGGTGTGCCTCTGTAAAAGGGGTCCGTGGTCTGATAGAGGGGCCATGCTAGGGCGCCTGATTTAAGAATGGGTGGT GGTCATCTCTGATAGTCCACAGGCATTTGAAGTTCCCTGTGGGGAGGAAGAAACC |
| mm4_exon_D030018L15Rik_chr15_96075447 |
CTTCAGCAGAGACCGATGCACCACAACAGCAGGTACAGCAGTGAACTTCTGACCATGAGAGGACAAGGGTTGAATATGAC CCCAAGCATGGCAGCTCCCGCTGGTCTACCAGCAGCCATGAGGAATC |
| mm4_intron_Tgtp2_chr11_49063719 |
GGACTCCAAGGGCATCTGAGTATCCCAACCTGGCATCTTTCTCCAAGCTTGACTGCTCCAGAAGACAAGCATGGTCCACA AAACACAGGTGGAAGGTAGGGGTT |
| mm4_intergenic_Tgtp1|Tgtp1/Gm12185_chr11_48991760 |
AGCACAAACTCCAAGGGCATCTGAGTATCCCAACCTGGCATCTTTCTCCAAGCTTGACTGCTCCAGAAGACAAGCATGGT CCACAAAACACAGGTGGAAGGTAGGGGTT |
| mm4_intergenic_Ifi47|Ifi47/Olfr56_chr11_49080789 |
GGACTCCAAGGGCATCTGAGTATCCCAACCTGGCATCTTTCTCCAAGCTTGACTGCTCCAGAAGACAAGCATGGTCTATA ACACACAGGTGGAAGGAAGGGGTTGCCTCCTCTTC |
| mm4_intergenic_Gm23796|Gm23831_chr3_39779737 |
TCTTTCCATTGTGCTCTGCTCCTAGCATGTCTGCACCAACAGAGGACAAAGGAAAAAGAAATCCACAATGATCTAACTTG AGGTGTATGTGTATTGCTAAATGCGATTATTTGGTGGTCTGC |
| mm4_intergenic_Gm25682|Adgb_chr10_10256165 |
CCCAAAGACAAGCATCGCTGACCTGATATTCTGAATTCTGCATCAGCTGCCATGCTTGGTCACTTGAATGCTTTACCTGA ACACTGGTACAAGTGTACCTTCAGGCTCACAGT |
| mm4_intergenic_Gstp2|BC021614_chr19_4056381 |
AGCCTCGGGACACTCTCTAGGAAGGTGGCAGGTGGCAGCAAATAGCAGCTGCTGGAGAGGCAACGCTAGGAGATGGTGGG AAACTCCCAGACTTCCTGCC |
| mm4_intron_Jph2_chr2_163355890 |
CGAGCACTCAGGGGAAGATGGGAGGTGGGAGGGACAGTCGAGGCAAGACCTGAGAGGTGGACTGGAGGGGCCATGCTGGG TACATGGGGGATGATGGCTGAGCACAGAGGTT |
| mm4_intergenic_Grtp1|Adprhl1_chr8_13213725 |
CTGACAGAGGGCAGGAAGTGGCTAAACGAGAGTGCTTTTCCATCCTCTCAAGGCAGAACAAAACCACAATACTCCCCTCT GCTGAAGGGGCCATTTTGGGGGCTGGGCCAGGGGCCGGAAGTATGTTC |
| mm4_intron_Tmem132e_chr11_82403626 |
GCCATAAACCTCAGCCAGCTCCCACCATGGCTGCTCCAGCTGGGTAATCCATCCAAGGCTTCAGCCTCTTCTCAGACAAG TCACACCTAGCTAGATTGTCTCTGTTCCAGGCATGGCTCACTAGTC |
| mm4_intron_Pbx3_chr2_34174796 |
TCCCATCTGAAGTAAACATCATTCCTCTTTTATCCAAATTTATAAACAACAAATGTACAATATTTCTCCCCTGCATGGCA GCTGCAGGAGAGGTTGTCAACGAGCACTCTT |
| mm4_intergenic_Itgb2l|Pcp4_chr16_96452273 |
CCTGAGGAGCAAGTGTCTGCGTCCGGTGCCCATCATTGCCTCTCCAGCAAACATCCACACACACCTAGACTTCTTCAGAT AACAGGAGTGGATTGTGAAGAGCTTCCACTGAGCTCCCAACAGGCATGAGAA |
| mm4_intergenic_Grik3|2610028E06Rik_chr4_125716512 |
TATGCAAATGAGGCCGCTGATGAGGCCTTTCTGCTGGGTGGCCCATGCTGGGACCAGCTCCACTGAGGGGATTTATTGCC CGCCTCAGCCCTACACAGGATCCA |
| mm4_intron_Nxn_chr11_76296154 |
CCCGACTTGCCAAGAAAAGCTATTCATTTGTGTTCTCCAATCATGGCCCCTCCTCCAGACAGCTCTGTCTCCGAGGTGTG TCACACCAAAGCAGAGGGAGGAG |
| mm3_intergenic_Gramd1b|1700110K17Rik_chr9_40321603 | CTGGCCAATGTCCTGCAGTAACCCCAGGCCTAGCCTGGCCTCACCAGCAGATGTTTCCTGCAATGGAAAGTTGCTGGC |
| mm4_intron_Zfp423_chr8_87738186 |
TAACTGTGGTAGCCAGCCAGCTGCGTGGGCCTGCCCTTTGCTCTGGCACTTGCCCTCGTCTCTCCTGCAGCTGACATGCT GGGAACCAGGGCCACAGTGTTCCCATCCTGTGTCCTCC |
| mm4_intergenic_Ggact|Pcca_chr14_122859164 |
ACGAAGGGTAGTCATTCTTGTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNCTGGTGCGTACATGCTTGGAGTGGATTAT GCTGAGTACCTTTGAAAACTACTGTCACACATCACTTAAAGAGAGGACACA |
| mm4_intron_Pard3_chr8_127144807 |
TGAGTCCTCAGGAAGTGGGTGGGTCAGCAGGAGCTGCCATGCGGGGATTCCTCAATATGTCTGATGGTTTTCACCTTAAG AGTCTGCCGGG |
| mm4_intergenic_Abhd6|Rpp14_chr14_8063029 |
CCACTCCCTCCACTGTCAACAGAAAACCTGGAATAGAAGTCATGAACCAAGAATATGAGTTGTTTTTGATAAACACATAA AATAGGTTTTCCAGTTCTCTGCTGGTTCAGCCATGCCGGGAGGTCCCTTGCACCCTGTTT |
| mm3_intron_Erc2_chr14_28088633 |
AGACCTGAGGCTCACACAGAATTTAATCCAATGTATTTGTTCAATGAAATCATCATTATCACGAGGGTAGAGTCTGCTGG AATGGCCAGGCTTGGAGTGGGGTAAGCAGACATTTGC |
| mm4_intron_Hdac4_chr1_92000817 |
GAACCCCTCCATGGCAGAAGCCCAGGCTGACCTCTCCAGCAGAGTCTGCAGAGAACAGCCCAGGCTGAGGCTCTCACGAT TACTCTTCATTCCACCTCATGGGCCACCTATTTCACCCTC |
| mm4_intergenic_Atp2a2|Gm22965_chr5_122503042 |
CACACAGCCTTTGACACACGTCTGCATAGAAGTCACAGGTTATGCTGTAGCAGCTATGCTAGGTGGCTTGGCTTTGGCTT CCAAATGATGGCTGCTATATAAGACTTCCACTTCCCCCAATGCGAGAGAAGGAGCCT |
| mm4_intergenic_Gm12122|Slit3_chr11_35493432 |
GCCCTTGTGGACTGTCCTACAATTTCTCCTAGCATAGCCGCTACTGCATAGCTGATCACTTTCCATCCAGCTTCCCCCAT GCTGGGGAGCTGGCTTGATCTAGC |
| mm4_intergenic_Gm24240|Rpl21-ps7_chr17_83287492 |
AGCCTGCAGATCAGCTCATCAGCGTCCTAGGATCCCATGGGTGAAGACAAGGTTAGCTGTTCTGCTTGCCTGGCCCCACC ATGGCCACTCTAACAGAAGTTTTCTGGAGGCCATGTCTTCCT |
| mm4_intron_Pdxk_chr10_78463955 |
TGCTCAGTGACCAGCCAAAAAAGATACATTGCAAGAGGCCACCACCTGGTTGCCCAGGCCAGCCCAATCTTCCTGCTGGA CCGGCCAGGCAAGGAAGCTCCAGTCAGCTGATATTGC |
| mm4_intron_Pdxk-ps_chr17_32099386 |
AAGGTACATTGCAGGAGGCCACACCTGGCTGCCCAAGCCAGCCCAATCTTCCTGCTGGACCGGCCAGGCAAGGACGCTCC AGTCAGCCTGACATTGCCTCTG |
| mm4_intergenic_Gm17276|Notch3_chr17_32114804 |
CAGAGGCAATGTCAGGCTGACTGGAGCGTCCTTGCCTGGCCGGTCCAGCAGGAAGATTGGGCTGGCTTGGGCAGCCAGGT GTGGCCTCCTGCAATGTACCTT |
| mm4_intergenic_Lef1|Hadh_chr3_131228947 |
CCCCTGACCTCCACATTTGGAATCCTTTTGGTCCTAATACAAGGGTGCCAAGCATTGCTCGTCCAGCAGAGCCATCCCCG ACCTAGCTGTGCTCACCCAAGAC |
| mm3_intergenic_2310007B03Rik|E030010N08Rik_chr1_93182610 |
AGACCCAGCCCCGAATTATGCCCCAGCACACTGGTGGGACAGGGTGAGGGTCTTGGTGTCCAGCATGGCCTCTCCAGGTG ACACTACAATCCATTATAATTGAAGCCCCTTGACCCCAACTGTCACA |
| mm4_intron_Ccdc67_chr9_15573241 |
CACAGTATGCCCACCACGGCCCCTCCTGCACAGAATGCCCACCATGGCCCCTCCTGCACAGAATGCCCACCATGGCCCCT CCTGCACAGAATGCCCACCACGGCCCCTCCTGCACAGTATG |
| mm4_intergenic_Gm7589|Adpgk_chr9_59260037 |
TGAGAGGGAGGGAACAAAGCTACTAGGCCTTGCCCTCCCCTCCTTGGCTAGGAATCCCAGACTTGCTGCTCCAGCAGATT TCCAGGGATGGGAGGAATCGATGATGCTCAGAGCATTTACAGCCAGTGGGAAAAGCC |
| mm4_intergenic_Fbxl14|Wnt5b_chr6_119522367 |
CCCTCCTCAAAGGCTGACTGTCCTGAATCTGCATAGACTCCCCACAGTGCTGGTTCCATGCCTGGCAGCTCCATCAGAGA AGGTATGCTCATCTGACACAAATTCACCCAGACCTGCGTTGGGCATGCTGATAAA |
| mm4_intergenic_Rilpl1|Tmed2_chr5_124532637 |
GAGAATGGCTGCATCCGGTACTGATGTACCTTGGAACTGCTATGCTGGGGCTGCTATGCTGGGGTGGGAGGAGGGGAGGG GGCTGCACTGGGAGCTGTTGCACTATGG |
| mm4_intron_Lsamp_chr16_41777769 |
GTCAAGAGTGCATGGAGGCAAATCAGTGATAAGCTTCAGCTGGAGAGGCCATGGGAGGGGCCAGGCTGGTTGGAGCAAGG CAACCATCT |
| mm4_intergenic_RP24-225I21.1|Gm26662_chr9_34551380 |
GCACCAGCTGACAGGTTCTATCCTCTTGTAGGCTAAGCTGGAGGTTATCCTGTTTCATTCTGCTGGAGGGATGATGCTTG GATTGAGCTTGGTTGCTTCAATTGT |
| mm4_intron_Gapvd1_chr2_34678259 |
TTACTCGGTGCTGTGCTCTGCCAGGGCCATCTTAGCATCTGTTCTTCCATGATCTGTTGGAGCTGCCAGGCCAGGGCTCT GGACAACAATCCCGA |
| mm4_intron_Ubxn11_chr4_134119394 |
TGATTTGCCCTGGCCTAGTCCTGGGTAGGCGAGCTTCCCCCCCAAACTCACTTTTATCCTGACATTGCCCCTCCAGCAGA TCCCCACAGACACTGGGGCTGCTGCCGTAGCTGTGCCAGTGTCTCATGGGAA |
| mm4_intergenic_Gm22016|Cdc73_chr1_142475403 |
TGGCATACTGGAGTTCCCATTTTAACTGTTAACAAGGATATCATTGCTACTACCTGCACCAAAGCCTGGCATAGCCCCTC CAACAGAAGAGAGGAGGCCTTTCTATTCCAGT |
| mm4_intron_Syt1_chr10_108600781 |
ATGCAAGCCGTTCTCCTCAAAGGTAGCTGCTTGCTTCATTTTTGCATGCCTTCCGACTGACAGCTGGAGGGGCCCTGCTG GGATGGCAGGGAGTGTTTCATT |
| mm4_intron_Ptprm_chr17_67090781 |
CCTAAATCCAGGGCAGCAGGCACCCCCAGGCAGGGAGTAGCCTGTGCTTCCCCCACAGCTTCTGAGCCCCAGCAGGGCAG CTCCTGTAGAACTTTAGTTCAGCAACAATCAGGCCA |
| mm4_intron_Cux1_chr5_136500806 | TTCACAGGTACAGCCAGCTGGCCAGACGCAGCCCAGCAGGACCTCACCAGCAGAGAGGCCACCACTTCCACAGAGCTT |
| mm4_intron_Bend7_chr2_4736344 |
TATGCTGTGTGGAGCCCTTGGATCTCTTGAATGTCCTCCAAGGCCTTACAGGAAGCTGGCACCTGGCAAGGCCTCTTCAG CAGAGCTTGCTGTTCCTTATTTATTGGGGCTTCACTGAAGACTGCA |
| mm4_intergenic_Rnf165|8030462N17Rik_chr18_77604625 |
ACCATTTCAGCAGGTCCTCGATGACCCTCTCACTGGCAGGCGCGGTCAGTCACACCCTCTGCTGGACCAGCCCTTCTGGG TCACACTCATCTGCTGGCTCTGCTCTGGCAAGTTCTCCACGCATACCCTTAGGTGGCTCC |
| mm4_intergenic_Gm12804|Gm12805_chr4_111310953 |
CTTCTACCTTCCAGCCAGGCCAACTGGACTGAAGAATCTCTGAAGAGGAGGACAACCCACTCAAGCCAGTTGCCAAGCAT TCCCCCTCCAGCACACCCCTTATTCTTTCTGTTCATCCCTTCATGAGAATCCT |
| mm4_exon_Bcas1_chr2_170347482 |
TCCCTGTGCCCCAGAAGTAATTGTTTCCCCAAATCCTAGCAAGCCCTCTCCAGCAAATCACTTCCCCATTCACTGATAAC TTCCAAGGACCCTATTTGAAAACCAATTCTTGCCCATTCTTCTTTACAGCCC |
| mm3_exon_Heatr5a_chr12_51947800 |
ACCTTGCCCTTTCCATGAGGAATTCCCAAAGGACAGTGTGTCACTGAACCGAGCAAGGCCGCCACAGCAGAACTAAAGCC AGTCACTGCTTCTGGTGAAGACTTGAGTATTGCGAGCCGT |