CRISPOR

DNA break repair outcome predictions

Guide: CTCCCCCCCTCTGGAGAGTC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGACATGGTTATGTTTCTGGA`	(Wild-type sequence)
519	`GGTTTTCCCCCTCCCCCCCTCTGGAGAG---------------CCGAAAGGACATGGTTA`	`15 bp deleted → no frameshift`
288	`GGTTTTCCCCCTCCCCCCCTCTGGA-----------TGGAGAGCCGAAAGGACATGGTTA`	`11 bp deleted → frameshift`
261	`GGTTTTCCCCCTCCCCCCCTCTGGAGAG-------------AGCCGAAAGGACATGGTTA`	`13 bp deleted → frameshift`
255	`GGTTTTCCCCCTCCCCCCCTCTGG---------GGATGGAGAGCCGAAAGGACATGGTTA`	`9 bp deleted → no frameshift`
242	`GGTTTTCCCCCTCCCCCCCTCTGG----------GATGGAGAGCCGAAAGGACATGGTTA`	`10 bp deleted → frameshift`
213	`GGTTTTCCCCCTCCCCCCCTCTGGAG-----------------CCGAAAGGACATGGTTA`	`17 bp deleted → frameshift`
191	`GGTTTTCCCCCTCCCCCCCTCTGGAGA---------TGGAGAGCCGAAAGGACATGGTTA`	`9 bp deleted → no frameshift`
129	`GGTTTTCCCCCTCCCCCCCTCTGGA---------------------------CATGGTTA`	`27 bp deleted → no frameshift`
110	`GGTTTTCCCCCTCCCCCCCTCTGGAGA--------------------AAGGACATGGTTA`	`20 bp deleted → frameshift`
109	`GGTTTTCCCCCTCCCCCCC--------------------------GAAAGGACATGGTTA`	`26 bp deleted → frameshift`
103	`GGTTTTCCCCCTCCCCCC---------------------------GAAAGGACATGGTTA`	`27 bp deleted → no frameshift`
99	`GGTTTTCCCCCTCCCCCCCTCTGGAGAG----------------------GACATGGTTA`	`22 bp deleted → frameshift`
99	`GGTTTTCCCCCTCCCCCCCTCTGGA----------------------AAGGACATGGTTA`	`22 bp deleted → frameshift`
98	`GGTTTTCCCCCTCCCCC----------------------------GAAAGGACATGGTTA`	`28 bp deleted → frameshift`
96	`GGTTTTCCCCCTCCCCCCCTCTGG---------------------------------TTA`	`33 bp deleted → no frameshift`
94	`GGTTTTCCCCCTCCCC-----------------------------GAAAGGACATGGTTA`	`29 bp deleted → frameshift`
90	`GGTTTTCCCCCTCCCCCCCTCTGGAG------------------------GACATGGTTA`	`24 bp deleted → no frameshift`
89	`GGTTTTCCCCCTCCC------------------------------GAAAGGACATGGTTA`	`30 bp deleted → no frameshift`
86	`GGTTTTCCCCCTCCCCCCCTCTGGAGA-------------------------CATGGTTA`	`25 bp deleted → frameshift`
84	`GGTTTTCCCCCTCC-------------------------------GAAAGGACATGGTTA`	`31 bp deleted → frameshift`
84	`GG-------------------------------GGATGGAGAGCCGAAAGGACATGGTTA`	`31 bp deleted → frameshift`
80	`GG--------------------------------GATGGAGAGCCGAAAGGACATGGTTA`	`32 bp deleted → frameshift`
76	`GG---------------------------------ATGGAGAGCCGAAAGGACATGGTTA`	`33 bp deleted → no frameshift`
73	`GGTTTTCCCCC----------------------------------GAAAGGACATGGTTA`	`34 bp deleted → frameshift`
70	`GGTTTTCCCCCTCCCCCCCTCTGGAGAGT-----------------------------TA`	`29 bp deleted → frameshift`
69	`GGTTTTCCCC-----------------------------------GAAAGGACATGGTTA`	`35 bp deleted → frameshift`
66	`GGTTTTCCC------------------------------------GAAAGGACATGGTTA`	`36 bp deleted → no frameshift`
62	`GGTTTTCC-------------------------------------GAAAGGACATGGTTA`	`37 bp deleted → frameshift`
62	`GG-------------------------------------AGAGCCGAAAGGACATGGTTA`	`37 bp deleted → frameshift`
38	`GGTT-------------------------------------------------------A`	`55 bp deleted → frameshift`
34	`GG-------------------------------------------------ACATGGTTA`	`49 bp deleted → frameshift`
14	`GGTTTT-----------------------------------------------------A`	`53 bp deleted → frameshift`
13	`GGTTT------------------------------------------------------A`	`54 bp deleted → no frameshift`

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