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Contents:

PCR primers for off-targets of GAAGAGGAGCTAAGGCGACA AGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313742_F TCGTCGGCAGCGTCCGCATCACAAGGTGACCCTA 59.7 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313742_R GTCTCGTGGGCTCGGCTCCTAATCCCAGGCTCCCT 60.1 1.00
mm3_intron_Atp10b_chr11_43184705_F TCGTCGGCAGCGTCTGTGAGCAAGGGATGAAGGG 59.6 0.80
mm3_intron_Atp10b_chr11_43184705_R GTCTCGTGGGCTCGGACTGGTTAACAAGTCCCTGCT 59.2 0.80
mm4_intron_3010001F23Rik_chrX_152380647_F Primer3: not found at this Tm N.d. 0.57
mm4_intron_3010001F23Rik_chrX_152380647_R Primer3: not found at this Tm N.d. 0.57
mm4_intron_Fbxl19_chr7_127754714_F TCGTCGGCAGCGTCGGGGAGGGGTTCAGATTGTG 60.0 0.50
mm4_intron_Fbxl19_chr7_127754714_R GTCTCGTGGGCTCGGTACTGAGGCAAGGCGTTCTG 60.0 0.50
mm4_intergenic_Gm6999|Olfm4_chr14_79980013_F Primer3: not found at this Tm N.d. 0.48
mm4_intergenic_Gm6999|Olfm4_chr14_79980013_R Primer3: not found at this Tm N.d. 0.48
mm4_intergenic_Mitf|Gm765_chr6_98208764_F Primer3: not found at this Tm N.d. 0.48
mm4_intergenic_Mitf|Gm765_chr6_98208764_R Primer3: not found at this Tm N.d. 0.48
mm4_intergenic_Olfr1216|Olfr1217_chr2_89022892_F Primer3: not found at this Tm N.d. 0.47
mm4_intergenic_Olfr1216|Olfr1217_chr2_89022892_R Primer3: not found at this Tm N.d. 0.47
mm4_intron_Sipa1l3_chr7_29407832_F TCGTCGGCAGCGTCGTGTGAGGAAACAGCCCTGA 59.8 0.39
mm4_intron_Sipa1l3_chr7_29407832_R GTCTCGTGGGCTCGGTTCCTCAGTTGCAGCTCTCC 59.6 0.39
mm4_intergenic_Cd86|Ildr1_chr16_36672428_F Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Cd86|Ildr1_chr16_36672428_R Primer3: not found at this Tm N.d. 0.39
mm3_intergenic_Gm23459|Gm24634_chr14_106321212_F TCGTCGGCAGCGTCGTGATGGGCAGAGGTTCCAA 59.9 0.38
mm3_intergenic_Gm23459|Gm24634_chr14_106321212_R GTCTCGTGGGCTCGGTGCTCTTTCTGTCCTTTGCAG 58.7 0.38
mm4_intergenic_Gm5944|Gm14903_chrX_115573369_F Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Gm5944|Gm14903_chrX_115573369_R Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Gm13996|Abtb2_chr2_103552286_F TCGTCGGCAGCGTCGGGGCTTTTACAGTAGACCTCA 59.4 0.36
mm4_intergenic_Gm13996|Abtb2_chr2_103552286_R GTCTCGTGGGCTCGGGTGGGCTCATCAGTCAGCTT 60.0 0.36
mm4_intergenic_Gm25921|Prss28_chr17_25307919_F Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_Gm25921|Prss28_chr17_25307919_R Primer3: not found at this Tm N.d. 0.36
mm4_intron_Fam63b_chr9_70643646_F Primer3: not found at this Tm N.d. 0.33
mm4_intron_Fam63b_chr9_70643646_R Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Gm20641|Nrg3_chr14_38648475_F Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Gm20641|Nrg3_chr14_38648475_R Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Tmem2|Gm5514_chr19_21900217_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Tmem2|Gm5514_chr19_21900217_R Primer3: not found at this Tm N.d. 0.31
mm4_intron_Zfp385b_chr2_77749973_F Primer3: not found at this Tm N.d. 0.30
mm4_intron_Zfp385b_chr2_77749973_R Primer3: not found at this Tm N.d. 0.30
mm4_intron_Cep135_chr5_76635649_F Primer3: not found at this Tm N.d. 0.28
mm4_intron_Cep135_chr5_76635649_R Primer3: not found at this Tm N.d. 0.28
mm4_intron_Snx27_chr3_94538569_F Primer3: not found at this Tm N.d. 0.27
mm4_intron_Snx27_chr3_94538569_R Primer3: not found at this Tm N.d. 0.27
mm3_intergenic_5830416P10Rik|Dusp5_chr19_53492104_F Primer3: not found at this Tm N.d. 0.27
mm3_intergenic_5830416P10Rik|Dusp5_chr19_53492104_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Rtn4r|mmu-mir-6366_chr16_18158732_F TCGTCGGCAGCGTCACAATGGGGAACAACCTGGG 60.1 0.27
mm4_intergenic_Rtn4r|mmu-mir-6366_chr16_18158732_R GTCTCGTGGGCTCGGAAGTGAGGAGTGACTGTGGC 59.6 0.27
mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_88786947_F Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_2410088K16Rik|Sh3bp4_chr1_88786947_R Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Gm7722|Gm14609_chrX_46904872_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm7722|Gm14609_chrX_46904872_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm23003|Gm26369_chr16_55740034_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm23003|Gm26369_chr16_55740034_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm6317|Gm25383_chr13_78548600_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm6317|Gm25383_chr13_78548600_R Primer3: not found at this Tm N.d. 0.22
mm4_intron_Arhgap22_chr14_33318453_F TCGTCGGCAGCGTCAAACCTTTTCCTGGCTTGCC 59.2 0.21
mm4_intron_Arhgap22_chr14_33318453_R GTCTCGTGGGCTCGGGGGAGGGTTGTGTGGTACAG 59.9 0.21
mm4_intergenic_Gm26179|Gm23924_chr6_74399288_F TCGTCGGCAGCGTCCAGTTCCACTCCAGTATATTCATGA 58.1 0.20
mm4_intergenic_Gm26179|Gm23924_chr6_74399288_R GTCTCGTGGGCTCGGTCTGCAGTGACTATGTGGAACA 59.3 0.20
mm4_intergenic_Gm23892|Gm25382_chr10_16161979_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm23892|Gm25382_chr10_16161979_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Scn3a_chr2_65565609_F TCGTCGGCAGCGTCAGCAACCAGGAGAAGTGAGC 59.9 0.19
mm4_intron_Scn3a_chr2_65565609_R GTCTCGTGGGCTCGGTTGGACCCTGGATGCTACCT 60.2 0.19
mm4_intergenic_Gm24326|Gm25923_chr12_27831541_F TCGTCGGCAGCGTCCCTTCCCTCTGTCTTCCCCT 60.2 0.19
mm4_intergenic_Gm24326|Gm25923_chr12_27831541_R GTCTCGTGGGCTCGGCATGAGTGGAGATGGAAGAAAAGA 58.5 0.19
mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_9333271_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm26674/Sash1|Gm9930_chr10_9333271_R Primer3: not found at this Tm N.d. 0.18
mm4_exon_Anp32a_chr9_62374742_F Primer3: not found at this Tm N.d. 0.18
mm4_exon_Anp32a_chr9_62374742_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm15338|Hs3st4_chr7_124303239_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm15338|Hs3st4_chr7_124303239_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Rnf220_chr4_117311319_F TCGTCGGCAGCGTCGGAGGAACAAGGATGGGGTG 60.0 0.17
mm4_intron_Rnf220_chr4_117311319_R GTCTCGTGGGCTCGGCCCTCTACACCCCAGATCCT 59.7 0.17
mm4_intergenic_Gm13794|Gm13796_chr2_95516921_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm13794|Gm13796_chr2_95516921_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Btrc_chr19_45463758_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Btrc_chr19_45463758_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Tbc1d22a_chr15_86495468_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Tbc1d22a_chr15_86495468_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Jak1_chr4_101173845_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Jak1_chr4_101173845_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Myo3b_chr2_70145266_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Myo3b_chr2_70145266_R Primer3: not found at this Tm N.d. 0.16
mm4_exon_Gtf2e2_chr8_33775999_F Primer3: not found at this Tm N.d. 0.16
mm4_exon_Gtf2e2_chr8_33775999_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Herpud2_chr9_25148333_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Herpud2_chr9_25148333_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24954|Gm5272_chr3_6425249_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm24954|Gm5272_chr3_6425249_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Fut8|Gm24070_chr12_77762019_F TCGTCGGCAGCGTCGTCCTGATTCTGTCCTACAGCA 59.5 0.15
mm4_intergenic_Fut8|Gm24070_chr12_77762019_R GTCTCGTGGGCTCGGTACAGTCCTCTGGGTCCCTG 59.9 0.15
mm4_intergenic_Rpl13a-ps1|Sorcs1_chr19_50106527_F TCGTCGGCAGCGTCTATGGGGAGAGGGAAGGTCG 60.1 0.15
mm4_intergenic_Rpl13a-ps1|Sorcs1_chr19_50106527_R GTCTCGTGGGCTCGGCGTGGCGCTGTAATGATTGG 59.9 0.15
mm4_intron_Tpd52_chr3_8973426_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Tpd52_chr3_8973426_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Fam20b|Gm15428_chr1_156773489_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Fam20b|Gm15428_chr1_156773489_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Tnfaip3|Olig3_chr10_19036369_F TCGTCGGCAGCGTCTGAATCTACTGCCCCAGCTG 59.4 0.14
mm4_intergenic_Tnfaip3|Olig3_chr10_19036369_R GTCTCGTGGGCTCGGAAGAAAAGAGCACTTCACAGAAAA 57.4 0.14
mm4_intergenic_Gm6767|Cblb_chr16_51961532_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm6767|Cblb_chr16_51961532_R Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_4930583I09Rik|Gm25344_chr17_64847111_F TCGTCGGCAGCGTCGAGGCCATGGAAGTGGTCAA 59.9 0.14
mm3_intergenic_4930583I09Rik|Gm25344_chr17_64847111_R GTCTCGTGGGCTCGGGCAAAGGCTCTTGGTCCCTT 60.5 0.14
mm3_intergenic_Gm13660|Gm13655_chr2_75571796_F TCGTCGGCAGCGTCGCTGTGTGTCCTGGACTTGA 59.8 0.14
mm3_intergenic_Gm13660|Gm13655_chr2_75571796_R GTCTCGTGGGCTCGGATCAGTGAGCCCTCCCTTCT 59.9 0.14
mm4_intron_Tmem163_chr1_127658169_F TCGTCGGCAGCGTCAGGATGACTCGCCTGCTATT 58.5 0.14
mm4_intron_Tmem163_chr1_127658169_R GTCTCGTGGGCTCGGTGAGCCTTACCCTCTCTCCT 58.9 0.14
mm4_intergenic_Csf1|Eps8l3_chr3_107780219_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Csf1|Eps8l3_chr3_107780219_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Txlnb|Heca_chr10_17881240_F TCGTCGGCAGCGTCTCTCCTGAGATTCACAAGATCAAA 57.6 0.14
mm4_intergenic_Txlnb|Heca_chr10_17881240_R GTCTCGTGGGCTCGGACCTAACTCTGGGAAACAAGACA 59.2 0.14
mm4_intergenic_Bmper|Npsr1_chr9_23548917_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Bmper|Npsr1_chr9_23548917_R Primer3: not found at this Tm N.d. 0.13
mm3_intergenic_Gpr98|Lysmd3_chr13_81634426_F TCGTCGGCAGCGTCCGTGAGCTCTCAAGTCGGTA 59.1 0.13
mm3_intergenic_Gpr98|Lysmd3_chr13_81634426_R GTCTCGTGGGCTCGGCCAAGCCAAAATGTACTTCCGT 59.4 0.13
mm4_intron_Ptprd_chr4_77743409_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Ptprd_chr4_77743409_R Primer3: not found at this Tm N.d. 0.12
mm4_exon_Spty2d1_chr7_46999769_F TCGTCGGCAGCGTCTGAGATGCAGTACAAGCGCA 60.0 0.12
mm4_exon_Spty2d1_chr7_46999769_R GTCTCGTGGGCTCGGGGCCTCCAAAGAAAGACCCA 59.8 0.12
mm4_intron_Cdk14_chr5_5367784_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Cdk14_chr5_5367784_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Robo1_chr16_72750990_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Robo1_chr16_72750990_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm24302|6030458C11Rik_chr15_12750079_F TCGTCGGCAGCGTCTCCCAAACCACTATGGCAACT 59.5 0.11
mm4_intergenic_Gm24302|6030458C11Rik_chr15_12750079_R GTCTCGTGGGCTCGGCACTAGAGACAGCTGCTGGG 59.8 0.11
mm4_intron_Ntm_chr9_29818443_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Ntm_chr9_29818443_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Cacna1h|Tekt4_chr17_25470715_F TCGTCGGCAGCGTCCCCACACTCAGGACCAGATC 59.4 0.11
mm4_intergenic_Cacna1h|Tekt4_chr17_25470715_R GTCTCGTGGGCTCGGGGGTAGTGAAGGCTCCAAGG 59.7 0.11
mm4_intron_Rapgef4_chr2_72194520_F TCGTCGGCAGCGTCTTGGGCACTTGCTAAGAGGG 59.9 0.11
mm4_intron_Rapgef4_chr2_72194520_R GTCTCGTGGGCTCGGGTGCTCAGTGGTGTCACCTA 59.3 0.11
mm4_intergenic_Edn1|Phactr1_chr13_42493637_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Edn1|Phactr1_chr13_42493637_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_AC158354.1|Gm4804_chr12_15690538_F TCGTCGGCAGCGTCCTGAGATCCAACACCCCACC 60.0 0.10
mm4_intergenic_AC158354.1|Gm4804_chr12_15690538_R GTCTCGTGGGCTCGGTCTTTCCTCAACTTCCTGGTGA 58.9 0.10
mm4_intergenic_Wdr11|Gm23847_chr7_129913898_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Wdr11|Gm23847_chr7_129913898_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Nek2|1700034H15Rik_chr1_191856503_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Nek2|1700034H15Rik_chr1_191856503_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Slco6b1_chr1_96907401_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Slco6b1_chr1_96907401_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Lhx9_chr1_138836641_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Lhx9_chr1_138836641_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Fam19a2_chr10_123296445_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_Fam19a2_chr10_123296445_R Primer3: not found at this Tm N.d. 0.10
mm2_intergenic_Gm5555|Gpr125_chr5_49595150_F TCGTCGGCAGCGTCTGAGTACTTGTGAAGCAGGACA 59.3 0.10
mm2_intergenic_Gm5555|Gpr125_chr5_49595150_R GTCTCGTGGGCTCGGGGGCCTTCAGCTGGTAAGAG 60.1 0.10
mm4_intron_Mark1_chr1_184933330_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Mark1_chr1_184933330_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Sox5_chr6_144071150_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Sox5_chr6_144071150_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Ebf3_chr7_137310338_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Ebf3_chr7_137310338_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Kctd5_chr17_24053301_F TCGTCGGCAGCGTCGCAGAGCTCACCGTGACATT 60.6 0.09
mm4_intron_Kctd5_chr17_24053301_R GTCTCGTGGGCTCGGGGGCTGACAGCTGTCTTCTT 59.9 0.09
mm3_intron_Pard6b_chr2_168085139_F TCGTCGGCAGCGTCGATCCCAGCTGTCAGTGCAA 60.3 0.09
mm3_intron_Pard6b_chr2_168085139_R GTCTCGTGGGCTCGGCTGGTTTGTTTGTAGGCTCTCC 59.1 0.09
mm4_intron_Frem1_chr4_83011286_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Frem1_chr4_83011286_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Smoc2|Thbs2_chr17_14521589_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Smoc2|Thbs2_chr17_14521589_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Srxn1|Tcf15_chr2_152119540_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Srxn1|Tcf15_chr2_152119540_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Wwox_chr8_114936121_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Wwox_chr8_114936121_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Phb|Gm9796_chr11_95685779_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Phb|Gm9796_chr11_95685779_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Kmt2e_chr5_23447006_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Kmt2e_chr5_23447006_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm9630|Gm24080_chr15_14569478_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm9630|Gm24080_chr15_14569478_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Sntb1|Has2_chr15_56054737_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Sntb1|Has2_chr15_56054737_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Man1c1|Ldlrap1_chr4_134716340_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Man1c1|Ldlrap1_chr4_134716340_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_n-R5s30|Rbfox1_chr16_6746875_F TCGTCGGCAGCGTCGCTGCACCTCTGGAGTATCC 59.8 0.07
mm4_intergenic_n-R5s30|Rbfox1_chr16_6746875_R GTCTCGTGGGCTCGGTTGCACAATCCCTGACTGCA 60.1 0.07
mm4_intron_Rufy1_chr11_50393382_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Rufy1_chr11_50393382_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Vimp_chr7_66086053_F TCGTCGGCAGCGTCCGCTGACATTCATGACGCTG 59.9 0.06
mm4_intron_Vimp_chr7_66086053_R GTCTCGTGGGCTCGGCAAGGAAAGCGGTGGGAGAA 60.2 0.06
mm4_intron_Atp8a2_chr14_59965393_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Atp8a2_chr14_59965393_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Mir361|Chm_chrX_113079826_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Mir361|Chm_chrX_113079826_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Acpp|Mir2136_chr9_104359391_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Acpp|Mir2136_chr9_104359391_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Dgkh|Vwa8_chr14_78818032_F TCGTCGGCAGCGTCAGTGTTCTCTGACCTGCTTCTG 59.9 0.06
mm4_intergenic_Dgkh|Vwa8_chr14_78818032_R GTCTCGTGGGCTCGGTGTCATTCCCAATCCCACCTC 59.7 0.06
mm4_intergenic_Gm24208|Ddx1_chr12_13177879_F TCGTCGGCAGCGTCCACTTCCGACTGCTCCTGAG 60.1 0.06
mm4_intergenic_Gm24208|Ddx1_chr12_13177879_R GTCTCGTGGGCTCGGCCCTTGCCCTAGCTCTCATC 59.6 0.06
mm4_intergenic_4933433F19Rik|Dusp26_chr8_30746083_F TCGTCGGCAGCGTCCCCCTGACCATTGCCTGATT 60.0 0.06
mm4_intergenic_4933433F19Rik|Dusp26_chr8_30746083_R GTCTCGTGGGCTCGGGAATTGGAGGGTCCTGGCTG 60.3 0.06
mm4_intron_Zfhx3_chr8_108862261_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Zfhx3_chr8_108862261_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Adcy1_chr11_7083334_F TCGTCGGCAGCGTCACTGTCTTCGGATTGGTCGT 59.0 0.06
mm4_intron_Adcy1_chr11_7083334_R GTCTCGTGGGCTCGGGATGTTCATGAGGGCAGGGG 60.4 0.06
mm4_intron_Igsf21_chr4_140189462_F TCGTCGGCAGCGTCCAGAGGAAGAGCTGTGTGCA 59.9 0.06
mm4_intron_Igsf21_chr4_140189462_R GTCTCGTGGGCTCGGGCACCAGCAGACTCAGTTCT 59.9 0.06
mm4_exon_D3Ertd751e_chr3_41758674_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_D3Ertd751e_chr3_41758674_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Nrg1_chr8_31900609_F TCGTCGGCAGCGTCGGCTGGAGAGAGAATACAGCT 58.9 0.06
mm4_intron_Nrg1_chr8_31900609_R GTCTCGTGGGCTCGGGCTAACGGCCAAGCAAATGA 59.4 0.06
mm4_intron_Ptprs_chr17_56465159_F TCGTCGGCAGCGTCCCAAGACTTTGAGGCGAGGT 59.9 0.06
mm4_intron_Ptprs_chr17_56465159_R GTCTCGTGGGCTCGGGTGGTAGGAAAGACCCCAGC 60.0 0.06
mm4_intergenic_Gm16432|Desi2_chr1_178161182_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm16432|Desi2_chr1_178161182_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Adss|Gm16432_chr1_177958542_F TCGTCGGCAGCGTCGCAGGTCCTGGCAGACTTTT 60.5 0.06
mm4_intergenic_Adss|Gm16432_chr1_177958542_R GTCTCGTGGGCTCGGGCATACCTGAAACGGGACCT 59.7 0.06
mm4_intergenic_Glra2|Gm15236_chrX_165369426_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Glra2|Gm15236_chrX_165369426_R Primer3: not found at this Tm N.d. 0.06
mm3_intron_Jph2_chr2_163382044_F Primer3: not found at this Tm N.d. 0.06
mm3_intron_Jph2_chr2_163382044_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Atl2|Hnrnpll_chr17_79901266_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Atl2|Hnrnpll_chr17_79901266_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Tram2|Tmem14a_chr1_21217093_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Tram2|Tmem14a_chr1_21217093_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm7094|Gm4956_chr1_21273624_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm7094|Gm4956_chr1_21273624_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_5033411D12Rik_chr13_17517408_F TCGTCGGCAGCGTCGCCAGCTCCTAGCCCCTAAT 61.1 0.05
mm4_intron_5033411D12Rik_chr13_17517408_R GTCTCGTGGGCTCGGGCCTCATATCACCTTTCTGTACCT 59.8 0.05
mm4_intron_Fam168a_chr7_100833981_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Fam168a_chr7_100833981_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Tbp/Pdcd2|Prdm9_chr17_15536807_F TCGTCGGCAGCGTCCCATCATCCGGGCTTGTCC 60.5 0.05
mm4_intergenic_Tbp/Pdcd2|Prdm9_chr17_15536807_R GTCTCGTGGGCTCGGGTCTCAGAACGGGCGGAAC 60.7 0.05
mm4_intron_Llgl2_chr11_115838741_F TCGTCGGCAGCGTCCACTCACCTCCAACCCAGTC 59.9 0.05
mm4_intron_Llgl2_chr11_115838741_R GTCTCGTGGGCTCGGCACTAAGACCAGGACGTCCG 59.8 0.05
mm4_intergenic_Rhox9|Gm15474_chrX_37903495_F TCGTCGGCAGCGTCTAGTGGTTCTGGGTTGGCTG 59.6 0.05
mm4_intergenic_Rhox9|Gm15474_chrX_37903495_R GTCTCGTGGGCTCGGTCTGGTGGTGGTGAGAAGGA 60.1 0.05
mm4_intergenic_Rhox5|Rhox6_chrX_37825256_F TCGTCGGCAGCGTCTCTGGTGGTGGTGAGAAGGA 60.1 0.05
mm4_intergenic_Rhox5|Rhox6_chrX_37825256_R GTCTCGTGGGCTCGGTAGTGGTTCTGGGTTGGCTG 59.6 0.05
mm4_intergenic_Kap|Etv6_chr6_133968945_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Kap|Etv6_chr6_133968945_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Rbms3_chr9_116774963_F TCGTCGGCAGCGTCACTGATGGGTGGAGGAAGGT 60.1 0.05
mm4_intron_Rbms3_chr9_116774963_R GTCTCGTGGGCTCGGGTCCTAACACCAGCAACATGA 58.2 0.05
mm4_intergenic_Gm22546|Gm22194_chr10_11725045_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm22546|Gm22194_chr10_11725045_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Arhgap24_chr5_102519863_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Arhgap24_chr5_102519863_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Sulf1_chr1_12711099_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Sulf1_chr1_12711099_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Gm10860_chr14_67434755_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Gm10860_chr14_67434755_R Primer3: not found at this Tm N.d. 0.05
mm3_intron_Gm53_chr11_96252161_F TCGTCGGCAGCGTCCCTTGTTCCACCGCATTTCC 59.7 0.05
mm3_intron_Gm53_chr11_96252161_R GTCTCGTGGGCTCGGCACCAGGGAGGCCATTTAGG 60.1 0.05
mm4_intron_Ttc7_chr17_87358466_F TCGTCGGCAGCGTCAACACTGGTGCGCAGTGATA 59.9 0.05
mm4_intron_Ttc7_chr17_87358466_R GTCTCGTGGGCTCGGAGGAAGCCTTGCAGTGTCAA 59.8 0.05
mm4_intergenic_1700023C21Rik|Abca8b_chr11_109864192_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_1700023C21Rik|Abca8b_chr11_109864192_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22538|Bche_chr3_73623947_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22538|Bche_chr3_73623947_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Nxph1|Gm24104_chr6_9686289_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Nxph1|Gm24104_chr6_9686289_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Dock5|Nefm/Nefl_chr14_68021123_F TCGTCGGCAGCGTCTGTCTTTTGATTCCGGCCCA 59.8 0.04
mm4_intergenic_Dock5|Nefm/Nefl_chr14_68021123_R GTCTCGTGGGCTCGGGCTTCTGCCACCCTCTTCAT 60.0 0.04
mm4_intergenic_Vps37d|Mlxipl_chr5_135089419_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Vps37d|Mlxipl_chr5_135089419_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Krt33b|Krt34_chr11_100033384_F TCGTCGGCAGCGTCCTGACCCTCCTCTCCCTTGA 59.9 0.04
mm4_intergenic_Krt33b|Krt34_chr11_100033384_R GTCTCGTGGGCTCGGCCTGGGTACAGCAACTGGAG 60.0 0.04
mm4_intron_Ptpru_chr4_131781032_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ptpru_chr4_131781032_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Cacna2d2|Gm17141/Cacna2d2_chr9_107402895_F TCGTCGGCAGCGTCTTGCTGCTAATGGCCCTGAT 59.7 0.04
mm4_intergenic_Cacna2d2|Gm17141/Cacna2d2_chr9_107402895_R GTCTCGTGGGCTCGGGCAGGCCAGGTTAGGTACAG 60.1 0.04
mm4_intron_Fggy_chr4_95876797_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Fggy_chr4_95876797_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Ccdc113_chr8_95551295_F TCGTCGGCAGCGTCCGGGCAATGGAGATCTACGT 59.6 0.04
mm4_exon_Ccdc113_chr8_95551295_R GTCTCGTGGGCTCGGTTGTGGACAGAAGGAGGCTG 59.6 0.04
mm4_intergenic_Nup210|Hdac11_chr6_91133899_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Nup210|Hdac11_chr6_91133899_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_St3gal3_chr4_117949840_F TCGTCGGCAGCGTCGCTAGTCAGGCCACATGTGT 60.0 0.04
mm4_intron_St3gal3_chr4_117949840_R GTCTCGTGGGCTCGGTTTGAGGGCTGTCAGGGAAG 59.5 0.04
mm4_intron_Tox_chr4_6766911_F TCGTCGGCAGCGTCACAGTCACTCACACTCTGCC 59.6 0.04
mm4_intron_Tox_chr4_6766911_R GTCTCGTGGGCTCGGCTGCCTCTGCCTACCTAGGA 60.1 0.04
mm4_intron_Iffo2_chr4_139561645_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Iffo2_chr4_139561645_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Dnajb13_chr7_100512333_F TCGTCGGCAGCGTCAGAAGTGGGAGAGGGAAGGG 60.2 0.04
mm4_intron_Dnajb13_chr7_100512333_R GTCTCGTGGGCTCGGTGATGTTTTGTCCCCAGCCC 60.5 0.04
mm4_intron_Ankrd13c_chr3_157974665_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Ankrd13c_chr3_157974665_R Primer3: not found at this Tm N.d. 0.04
mm3_exon_Wnt9a_chr11_59332082_F Primer3: not found at this Tm N.d. 0.04
mm3_exon_Wnt9a_chr11_59332082_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Pabpc1_chr15_36606030_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Pabpc1_chr15_36606030_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Gm15046_chrX_140184935_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Gm15046_chrX_140184935_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Gfod2_chr8_105714559_F TCGTCGGCAGCGTCGAGGTGGGAAGTTGTCAGGG 59.9 0.04
mm4_exon_Gfod2_chr8_105714559_R GTCTCGTGGGCTCGGTGTTCGTGCTCAGAGATCGC 60.4 0.04
mm4_intergenic_Gm26831|Gm8321_chr1_119071382_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm26831|Gm8321_chr1_119071382_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Mogat1|Acsl3/Utp14b_chr1_78621178_F TCGTCGGCAGCGTCTGCGTGGGGTTCATAATGGT 59.6 0.04
mm3_intergenic_Mogat1|Acsl3/Utp14b_chr1_78621178_R GTCTCGTGGGCTCGGCTCATAGGCCTGCTTCCTCG 59.9 0.04
mm4_intergenic_Vmn2r93|Vmn2r95_chr17_18380468_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Vmn2r93|Vmn2r95_chr17_18380468_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Dscaml1_chr9_45739080_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Dscaml1_chr9_45739080_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm24478|Gm14255_chr2_162818664_F TCGTCGGCAGCGTCCCTGACACCCTGCTGTCATT 59.9 0.03
mm4_intergenic_Gm24478|Gm14255_chr2_162818664_R GTCTCGTGGGCTCGGCATTGCTTCTCCTGCAGGGA 60.0 0.03
mm4_intergenic_Ncoa1|Gm3625_chr12_4499942_F TCGTCGGCAGCGTCAATGGGGCCAAAGGATCTGG 60.0 0.03
mm4_intergenic_Ncoa1|Gm3625_chr12_4499942_R GTCTCGTGGGCTCGGCCCACCTGTCACTGGAAACA 59.8 0.03
mm4_intron_Hmbox1_chr14_64944040_F TCGTCGGCAGCGTCTGCATGAAGAGCCAACAGGG 60.6 0.03
mm4_intron_Hmbox1_chr14_64944040_R GTCTCGTGGGCTCGGCATAAGCCCTGAGAGCGCTT 60.1 0.03
mm4_intergenic_Fam43a|Gm26295_chr16_30794687_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Fam43a|Gm26295_chr16_30794687_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_4930555K19Rik|Oxr1_chr15_41420310_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_4930555K19Rik|Oxr1_chr15_41420310_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Pced1a/Vps16|Vps16_chr2_130434454_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Pced1a/Vps16|Vps16_chr2_130434454_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tmem108|Nphp3_chr9_103923061_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tmem108|Nphp3_chr9_103923061_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Acot7_chr4_152211332_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Acot7_chr4_152211332_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Cxadr_chr16_78338506_F TCGTCGGCAGCGTCTCACTGTGGCTTTCTACCCT 58.2 0.03
mm4_exon_Cxadr_chr16_78338506_R GTCTCGTGGGCTCGGAGAAGCACGGTCAAAGCGTA 59.9 0.03
mm4_intergenic_Gm11415|6030471H07Rik_chr4_84023029_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm11415|6030471H07Rik_chr4_84023029_R Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_2410076I21Rik|Hcn4_chr9_58818446_F TCGTCGGCAGCGTCCATGCCCCAGTAGAATCCCC 59.8 0.03
mm3_intergenic_2410076I21Rik|Hcn4_chr9_58818446_R GTCTCGTGGGCTCGGATCCCCTCTTCCTAGCACCC 60.4 0.03
mm4_intergenic_Gm4487|Gm24073_chr14_113838949_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm4487|Gm24073_chr14_113838949_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_6030443J06Rik|Gm21846_chr5_22770833_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_6030443J06Rik|Gm21846_chr5_22770833_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gas6|1700029H14Rik_chr8_13529668_F TCGTCGGCAGCGTCCAGCTCGTGGATGGACAGAG 60.1 0.03
mm4_intergenic_Gas6|1700029H14Rik_chr8_13529668_R GTCTCGTGGGCTCGGCTGTCCAAGGAGTGCTGAGG 60.0 0.03
mm4_intron_Lmx1a_chr1_167731242_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Lmx1a_chr1_167731242_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm11404|Gm11221_chr4_69484434_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm11404|Gm11221_chr4_69484434_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Fam163b_chr2_27140895_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Fam163b_chr2_27140895_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cd72|Sit1_chr4_43455045_F TCGTCGGCAGCGTCCCCAGGACATCACCCCAATC 60.1 0.03
mm4_intergenic_Cd72|Sit1_chr4_43455045_R GTCTCGTGGGCTCGGTGCAGAATGGGGCTCATGAA 59.6 0.03
mm4_intergenic_1700021F07Rik|Ankrd60_chr2_173541190_F TCGTCGGCAGCGTCAACCACAAGTGCTGGGCATA 59.8 0.03
mm4_intergenic_1700021F07Rik|Ankrd60_chr2_173541190_R GTCTCGTGGGCTCGGCCCCTTTGGCTCCTCCTTTC 60.3 0.03
mm4_intron_Btbd2_chr10_80655265_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Btbd2_chr10_80655265_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_4930529M08Rik_chr2_146024367_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_4930529M08Rik_chr2_146024367_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Bnc2_chr4_84655739_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Bnc2_chr4_84655739_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Plac1_chrX_53213557_F TCGTCGGCAGCGTCAGGGCTCATCTACCCAGTGA 59.6 0.02
mm4_intron_Plac1_chrX_53213557_R GTCTCGTGGGCTCGGGCCACCATGCCAACTTGATC 59.8 0.02
mm4_intergenic_Gm25714|Gm24997_chr3_44924064_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm25714|Gm24997_chr3_44924064_R Primer3: not found at this Tm N.d. 0.02
mm3_intron_Cacna1c_chr6_118639891_F TCGTCGGCAGCGTCAGTTGTCTGGCTTCCCAACC 60.1 0.02
mm3_intron_Cacna1c_chr6_118639891_R GTCTCGTGGGCTCGGGCAGAGATGCCTGGGAACTT 60.0 0.02
mm4_intergenic_5730522E02Rik|Gm22868_chr11_26296801_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_5730522E02Rik|Gm22868_chr11_26296801_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Sbspon|4930444P10Rik_chr1_15940618_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Sbspon|4930444P10Rik_chr1_15940618_R Primer3: not found at this Tm N.d. 0.02
mm3_intron_Igsf9b_chr9_27330408_F TCGTCGGCAGCGTCATCATTGGTGTGGGCAGAGG 60.0 0.02
mm3_intron_Igsf9b_chr9_27330408_R GTCTCGTGGGCTCGGTGCTGCCTATGGGAGACAAC 59.7 0.02
mm4_intergenic_Snx29|Cpped1_chr16_11776912_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Snx29|Cpped1_chr16_11776912_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm5435|Gm8358_chr12_82508903_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm5435|Gm8358_chr12_82508903_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_4930486L24Rik_chr13_60843761_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_4930486L24Rik_chr13_60843761_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm12004|4930554G24Rik_chr11_13939965_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm12004|4930554G24Rik_chr11_13939965_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Rnf182_chr13_43621406_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Rnf182_chr13_43621406_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Tff3|Tff2_chr17_31135724_F TCGTCGGCAGCGTCCTGGCAGAGGAAACACCACT 59.8 0.02
mm4_intergenic_Tff3|Tff2_chr17_31135724_R GTCTCGTGGGCTCGGGGCTTCCTGACTACCTCTGC 59.8 0.02
mm4_exon_A830092H15Rik_chr3_26331751_F TCGTCGGCAGCGTCGATGAAGGGATGCCAGGGTC 60.1 0.02
mm4_exon_A830092H15Rik_chr3_26331751_R GTCTCGTGGGCTCGGGTGGCCGAGTACATGCATCT 60.1 0.02
mm4_intron_Myo1e_chr9_70324241_F TCGTCGGCAGCGTCTGCATGGCCACTCTGAACTC 60.3 0.02
mm4_intron_Myo1e_chr9_70324241_R GTCTCGTGGGCTCGGTAGACCACAGCCACAGGATT 58.3 0.02
mm4_intron_Plxdc1_chr11_97930649_F TCGTCGGCAGCGTCTTGTGTGGCCCTTAGTGACC 59.8 0.02
mm4_intron_Plxdc1_chr11_97930649_R GTCTCGTGGGCTCGGAGCTGAGAGACTTGTGCGTT 59.6 0.02
mm4_intergenic_Rps18-ps2|Gm23423_chr4_9160620_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Rps18-ps2|Gm23423_chr4_9160620_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Slc7a1_chr5_148383645_F TCGTCGGCAGCGTCACATCAAGGCCAAAGCTGGA 59.8 0.02
mm4_intron_Slc7a1_chr5_148383645_R GTCTCGTGGGCTCGGAGCCAACAGGAAGGTGTGAG 59.8 0.02
mm4_intron_Trappc3_chr4_126275002_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126275002_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274787_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274787_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274723_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274723_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274631_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274631_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274569_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Trappc3_chr4_126274569_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_AC147224.1|Cdh12_chr15_21008356_F TCGTCGGCAGCGTCTCAGTGTCTGATGTGAGCTACT 58.5 0.01
mm4_intergenic_AC147224.1|Cdh12_chr15_21008356_R GTCTCGTGGGCTCGGTGGAGCCTTGATGAAGTTGGT 59.5 0.01
mm3_intron_Ccdc171_chr4_83575588_F Primer3: not found at this Tm N.d. 0.01
mm3_intron_Ccdc171_chr4_83575588_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Dcaf5|Scarna3b_chr12_80383787_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Dcaf5|Scarna3b_chr12_80383787_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Vmn2r102|Vmn2r-ps122_chr17_19704425_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Vmn2r102|Vmn2r-ps122_chr17_19704425_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Kcnj15_chr16_95297876_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Kcnj15_chr16_95297876_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Abhd2_chr7_79363190_F TCGTCGGCAGCGTCGCATCTCTGAGGCTGCTTCA 60.1 0.01
mm4_exon_Abhd2_chr7_79363190_R GTCTCGTGGGCTCGGCACGCGCCTTCTCTCTCTTA 59.5 0.01
mm4_intron_Nrp1_chr8_128421861_F TCGTCGGCAGCGTCCAGGAGCTGAGACGAAGGTC 59.8 0.01
mm4_intron_Nrp1_chr8_128421861_R GTCTCGTGGGCTCGGATCAGGGTGGTCTGAGGTGA 59.8 0.01
mm4_intergenic_Gm23708|G6pd2_chr5_61307125_F TCGTCGGCAGCGTCGGCCTACACTTTGATCAGCG 58.9 0.01
mm4_intergenic_Gm23708|G6pd2_chr5_61307125_R GTCTCGTGGGCTCGGAGGCAAGGATCATAGCATGTGT 59.8 0.01
mm3_exon_Tenm1_chrX_42657541_F TCGTCGGCAGCGTCTGCTAACAGAGTTTCCTGAAGGA 59.3 0.01
mm3_exon_Tenm1_chrX_42657541_R GTCTCGTGGGCTCGGAATGGTCCAGCCCACAACAA 60.1 0.01
mm4_intergenic_Gm22284|F630206G17Rik_chr11_45743626_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22284|F630206G17Rik_chr11_45743626_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_n-R5s209|Gm7846_chr1_27020969_F TCGTCGGCAGCGTCTGCATCACTAAAGGCAGTTGT 58.1 0.01
mm4_intergenic_n-R5s209|Gm7846_chr1_27020969_R GTCTCGTGGGCTCGGAAGGGATGGAGGGATCCACA 59.9 0.01
mm4_intergenic_Chchd2|2410018M08Rik_chr5_129887473_F TCGTCGGCAGCGTCGAAAAGCTCGGGCGCTAAAG 59.9 0.01
mm4_intergenic_Chchd2|2410018M08Rik_chr5_129887473_R GTCTCGTGGGCTCGGGAGTGCAACCCCAGGAACTT 60.1 0.01
mm4_intron_Ttll11_chr2_35949057_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ttll11_chr2_35949057_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Vrtn|Syndig1l_chr12_84674973_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Vrtn|Syndig1l_chr12_84674973_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fam169b|Gm5334_chr7_68585491_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fam169b|Gm5334_chr7_68585491_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm15338|Hs3st4_chr7_124366641_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm15338|Hs3st4_chr7_124366641_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Aire_chr10_78031719_F TCGTCGGCAGCGTCGCCAGCACCCTACATGGAAA 60.3 0.01
mm4_intron_Aire_chr10_78031719_R GTCTCGTGGGCTCGGCTAGTCTGGGCTGTGCTCAC 60.1 0.01
mm4_intron_Begain_chr12_109044782_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Begain_chr12_109044782_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Muc6_chr7_141648539_F TCGTCGGCAGCGTCGGTGTCTAGGGCTGCATCTG 60.1 0.01
mm4_intron_Muc6_chr7_141648539_R GTCTCGTGGGCTCGGCCTGAAGAAGGGCAGTGTGT 59.8 0.01
mm4_intron_4930516K23Rik_chr7_104023750_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_4930516K23Rik_chr7_104023750_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Caln1_chr5_130596339_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Caln1_chr5_130596339_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Tln1_chr4_43554939_F TCGTCGGCAGCGTCCTGAAAGGGCGATGACCCAT 60.1 0.01
mm4_exon_Tln1_chr4_43554939_R GTCTCGTGGGCTCGGACCCTGTTATGGCTTGGGAC 59.6 0.01
mm4_intron_Ptpn14_chr1_189813774_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ptpn14_chr1_189813774_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rpl9-ps4|Gm20554_chr13_72487319_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Rpl9-ps4|Gm20554_chr13_72487319_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cdc42bpa_chr1_179993049_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Cdc42bpa_chr1_179993049_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Mphosph9_chr5_124253442_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Mphosph9_chr5_124253442_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Smg7_chr1_152846130_F TCGTCGGCAGCGTCCACCAAGGCCTCAGACAGAA 59.6 0.00
mm4_exon_Smg7_chr1_152846130_R GTCTCGTGGGCTCGGCGCCTGTCACTCAAACTCCA 60.2 0.00
mm4_intergenic_Kif2b|Gm11498_chr11_91748566_F TCGTCGGCAGCGTCGCAATCCTTCCTCAGGCTGT 60.0 0.00
mm4_intergenic_Kif2b|Gm11498_chr11_91748566_R GTCTCGTGGGCTCGGGGTCCTCAGGTTGCTGATAGG 59.8 0.00
mm4_intergenic_Ankrd55|Il6st_chr13_112392537_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ankrd55|Il6st_chr13_112392537_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22140|Gm5908_chr8_32283009_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22140|Gm5908_chr8_32283009_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_1500016L03Rik|1700109G15Rik_chr11_84684747_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_1500016L03Rik|1700109G15Rik_chr11_84684747_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313742 CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCTCCGCGGTCGCCGTCGACACGGCGCTGGGGCTACCCGGCGCCTG
CCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAG
mm3_intron_Atp10b_chr11_43184705 TGTGAGCAAGGGATGAAGGGGCTACAGGAGCTTGTTTTGCAAAGAATATGGAAGAGGAGATAAGGAAACATGGAGCAGTG
GTCTACTAAAATATCAAAAAGTGAAGCAGGGACTTGTTAACCAGT
mm4_intron_Fbxl19_chr7_127754714 GGGGAGGGGTTCAGATTGTGACTGGAGAGGAGATGAGGAGACAAGGTCTTGCTGTGAACTCAGAACGCCTTGCCTCAGTA
mm4_intron_Sipa1l3_chr7_29407832 GTGTGAGGAAACAGCCCTGAGTGGGACAAAGAGGAGCCAGGGAGACAGGGAAAGTGGGGTCACTCCTCAGGGTGGTCACC
CTTATAAAGGAAGGAAATGCAAGATGAAGGAGAGCTGCAACTGAGGAA
mm3_intergenic_Gm23459|Gm24634_chr14_106321212 GTGATGGGCAGAGGTTCCAAGTGAATTCGTAGAAAAAGAGGTGCTAAGGAGACAGGGCTGCAAAGGACAGAAAGAGCA
mm4_intergenic_Gm13996|Abtb2_chr2_103552286 GGGGCTTTTACAGTAGACCTCAGTAAGATCCAGAGGAGCTAGGGAGACATGGTATAAACAAGGAGGCTGCAAGCTAATAA
TTATGAAAGCTGACTGATGAGCCCAC
mm4_intergenic_Rtn4r|mmu-mir-6366_chr16_18158732 ACAATGGGGAACAACCTGGGGCTGGTCAATATAAAGAGGAGCAAAGGAGGCAGGGCTTAGAGACCCTGATCCCATCACCC
TGGGCCACAGTCACTCCTCACTT
mm4_intron_Arhgap22_chr14_33318453 AAACCTTTTCCTGGCTTGCCCCAGTCAGCAATCCTCCCTGTCTGCTTAGCTCTTCTGCCCACACTGGACAGCTTTGGTCT
CCCAGCACACTGTACCACACAACCCTCCC
mm4_intergenic_Gm26179|Gm23924_chr6_74399288 CAGTTCCACTCCAGTATATTCATGAGTTTAATTTCTAGAGGAGCTAAGGCAAAAAGGTGAGAAAAATCATATGCATTTGT
CTCAGGAAGTTTGTTCCACATAGTCACTGCAGA
mm4_intron_Scn3a_chr2_65565609 AGCAACCAGGAGAAGTGAGCATCCAAGCCAGATGAGCAGCTAAGGAGGCACGGCAACTTTAAAAACACAATCAAACAGGT
AGCATCCAGGGTCCAA
mm4_intergenic_Gm24326|Gm25923_chr12_27831541 CCTTCCCTCTGTCTTCCCCTTTATCTCCATTTTCCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNCTTACCCCATCTTCTCTTTTCTTCCATCTCCACTCATG
mm4_intron_Rnf220_chr4_117311319 GGAGGAACAAGGATGGGGTGCAAAGGAAAGCAGGAAGGAGGAGGAGGAAAGGAGACAGGGTAGTTTACACAGACAACTAG
CTAGGATCTGGGGTGTAGAGGG
mm4_intergenic_Fut8|Gm24070_chr12_77762019 GTCCTGATTCTGTCCTACAGCAAATCCATGTCATCTTGGCTCCTCTGCTCTGAGGCTGGCCCCCTGTTGATTCCATAGGA
AGCAGCACCCATCAGGGACCCAGAGGACTGTA
mm4_intergenic_Rpl13a-ps1|Sorcs1_chr19_50106527 TATGGGGAGAGGGAAGGTCGGTCTCATCGACCCAATCATAGGCTACCAATCCCTGGGCACAGAGCAAAAGCAGAGGAGAT
AAGACCACAGGG
ACCATCTGTCCACTTTAACAAAGGGGCCAATCATTACAGCGCCACG
mm4_intergenic_Tnfaip3|Olig3_chr10_19036369 TGAATCTACTGCCCCAGCTGTGCTTTCTTGTCTGTCTGTTTCTGTACCTTTCACACCCTTATGCCTTAGCCCCTCTTCCT
GCACTCAATTTCTTACTATTTTTCTGTGAAGTGCTCTTTTCTT
mm3_intergenic_4930583I09Rik|Gm25344_chr17_64847111 GAGGCCATGGAAGTGGTCAATTCACCAGAAGGTGGGAAGCTAGCAAGGACTTGTGATATTTATGTGTCAGAAACCCTGTG
GCCTTAGCTTCTCCTC
GAAGGGACCAAGAGCCTTTGC
mm3_intergenic_Gm13660|Gm13655_chr2_75571796 GCTGTGTGTCCTGGACTTGATAGATACATAAACTCACATATGCACAGATGACACAAGGAAGAAGAGAAGCTAAGGGAACA
AGG
GGGACCAGGAGAAAGAAGGGAGGGCTCACTGAT
mm4_intron_Tmem163_chr1_127658169 AGGATGACTCGCCTGCTATTTATATACATATAAGGATCACTACAGAGGTAGATTGTACCCTTTGAGAAGGGGAGCACAGG
AGACATGG
AGGAGAGAGGGTAAGGCTCA
mm4_intergenic_Txlnb|Heca_chr10_17881240 TCTCCTGAGATTCACAAGATCAAAGGAAAAGGAGATGAGGCTACAAGGAGACCCTTGAAATAAAAATTACCTTCCAATGA
GTTCTTTGTCTTGTTTCCCAGAGTTAGGT
mm3_intergenic_Gpr98|Lysmd3_chr13_81634426 CGTGAGCTCTCAAGTCGGTAGCATAGTAAGAGGATTTAAGGCGACAGAGAATCAAAATCATGAGCCAGTTATCCAGATAA
TCATAAAGCAAAACAAAAAATGGGAAGACGGAAGTACATTTTGGCTTGG
mm4_exon_Spty2d1_chr7_46999769 TGAGATGCAGTACAAGCGCATACCTTTTTGTCTAAGCTCCTCTTCTTTCCGCCTAAGGAAAGCTTGCACAGCTGCCGACT
GGACACCTTTCACTTTTGGGTCTTTCTTTGGAGGCC
mm4_intergenic_Gm24302|6030458C11Rik_chr15_12750079 TCCCAAACCACTATGGCAACTAGCACCCCCACCCCCACAGCCAACCAGTGTCCCTGTGTCCTCAGCTGCTCTTCTTAACA
TTCCTTTCCTCTCCAGACTTCCACCGAAGATCCAGCCTCCCCCAGCAGCTGTCTCTAGTG
mm4_intergenic_Cacna1h|Tekt4_chr17_25470715 CCCACACTCAGGACCAGATCATGGAAGAACATCTCAGAAAAGGAGCCAAGGAGCCAGGGAGGAGGGCAGGGGTGTCAGGG
ACCTTGGAGCCTTCACTACCC
mm4_intron_Rapgef4_chr2_72194520 TTGGGCACTTGCTAAGAGGGCCTGGCTCCTCTGGCATCCCTGTGGCCCCAGCTTCTCTTCGTTATCTTTTGAGGTCCTGT
GCTTTCTAGGTGACACCACTGAGCAC
mm4_intergenic_AC158354.1|Gm4804_chr12_15690538 CTGAGATCCAACACCCCACCATGGGACTCAATGTTGAAAGCACTGAATTTCAATGCAGAGTGAGAAGAAAAGATAAGGCG
AGAAGG
TGACTCCTCACCAGGAAGTTGAGGAAAGA
mm2_intergenic_Gm5555|Gpr125_chr5_49595150 TGAGTACTTGTGAAGCAGGACAGAAAGAAGAGAAGAGGAGCTAGGGGGACATGGCAAGGGAAGGATCCTGTCACCTTACA
GACAAGTTGAAGTCTGTAGAGCAAGAGACGGGTTGAAGTCTCTTACCAGCTGAAGGCCC
mm4_intron_Kctd5_chr17_24053301 GCAGAGCTCACCGTGACATTTATGCTTAATCTGAGTCACTGCCTTGGTGCCTCAGCTCCTCTTGGTCACTCTGGTGTGAA
GGCAGGAAGTAAGGAAGAAGACAGCTGTCAGCCC
mm3_intron_Pard6b_chr2_168085139 GATCCCAGCTGTCAGTGCAAGCTCAAGGGCAGCTAAGGCGACACAGGGAGAGCCTACAAACAAACCAG
mm4_intergenic_n-R5s30|Rbfox1_chr16_6746875 GCTGCACCTCTGGAGTATCCTGGAAAAAGAGGAGCTCTGGAGACAGGGAAGGCTCCTGCAGTCAGGGATTGTGCAA
mm4_intron_Vimp_chr7_66086053 CGCTGACATTCATGACGCTGGGATTCTTGGTTGCGGAAAAGTGGCTCCTGTCCACGGAGCAGGAAGCGGAGCAAAGGCCA
CGTGG
TGGGTTCTCCCACCGCTTTCCTTG
mm4_intergenic_Dgkh|Vwa8_chr14_78818032 AGTGTTCTCTGACCTGCTTCTGTACAGTAAATATTTAGTAAACATTCCAAATAAAATATGCTTCTTTCTTTTTATGAAGA
GGAAGTCAGGAGACAAGG
AGAAATGAAGAAGAGGTGGGATTGGGAATGACA
mm4_intergenic_Gm24208|Ddx1_chr12_13177879 CACTTCCGACTGCTCCTGAGAGGTCCTGGCCTCCTCAGCTCCTCTTCTGGCTCCATGGCCCTTCCTTATGAAGATGAGAG
CTAGGGCAAGGG
mm4_intergenic_4933433F19Rik|Dusp26_chr8_30746083 CCCCTGACCATTGCCTGATTCATTCCCTTCCCTGACACCTTAGATCCTCTCCTAGCTTCCCTGTCAGCCAGGACCCTCCA
ATTC
mm4_intron_Adcy1_chr11_7083334 ACTGTCTTCGGATTGGTCGTCTTGACCCTGTCACCATAGCTGCTCTTACGCAAGTTCAGTTACTTTCTATTGTGCTGGCA
TTGTGTGCTGCCCCTGCCCTCATGAACATC
mm4_intron_Igsf21_chr4_140189462 CAGAGGAAGAGCTGTGTGCAGGGGCGTCCTCAGGGGTGTCTCCTGGGACAGCAACTGTGAGCCATGGAGAAGGAGCAAAG
GGGACAGGG
TTGGTCCCCGGGTACTATGAAACAGAACTGAGTCTGCTGGTGC
mm4_intron_Nrg1_chr8_31900609 GGCTGGAGAGAGAATACAGCTACCAGCATCTCAGGTTATTGTCTTTGCAACATCAGAGGAGGAGGAGAAGCCAAGGGGAC
AGGG
ACAGAATAAACTGGGAATTCCCTGTAGAAATAGTTTTCATTTGCTTGGCCGTTAGC
mm4_intron_Ptprs_chr17_56465159 CCAAGACTTTGAGGCGAGGTTATATCCAGTGCTGCTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNGCTTCTCCACCATGCTGGGGTCTTTCCTACCAC
mm4_intergenic_Adss|Gm16432_chr1_177958542 GCAGGTCCTGGCAGACTTTTGTCTCCTTTTGAGGAAACAAGTACTTCAATCCAGAAAGTAAGCTCTCTATGCCAAATGCT
TGGCCATGTCGCCATTGTGCCTCTTCCTGTGAGGTCCCGTTTCAGGTATGC
mm4_intron_5033411D12Rik_chr13_17517408 GCCAGCTCCTAGCCCCTAATAATTTAAGGAAGTAAAGTACATAAGACATTAGCTATTGTGAGTAAGAATGAACAAGGTAG
AGGAAGGAAGAGAAGATAAGGGGAAAAGGGTAGGTACAGAAAGGTGATATGAGGC
mm4_intergenic_Tbp/Pdcd2|Prdm9_chr17_15536807 CCATCATCCGGGCTTGTCCGTCCCACCTGCGTCCCAGGCGGTGGAACTGGCTCCCCCGTCCCCCTTGACTCCTGAGCGCC
TCTTC
TCTCCCTTCCCCCGTTCCGCCCGTTCTGAGAC
mm4_intron_Llgl2_chr11_115838741 CACTCACCTCCAACCCAGTCAGGCAGCTTAGGTTCCCACTGAAGACTAGCTAAGGGAACAGGGACTACCTCAGGGGCTGG
ACGGACGTCCTGGTCTTAGTG
mm4_intergenic_Rhox9|Gm15474_chrX_37903495 TAGTGGTTCTGGGTTGGCTGTGTCCACTCCAACCCTCCTTGTAGCCCTAGCACCTCTGCAACATATGTGAGCAACTTGTA
AACTTGAACTTACTTGAACTTGGGTGCTTCCTTCTCACCACCACCAGA
mm4_intergenic_Rhox5|Rhox6_chrX_37825256 TCTGGTGGTGGTGAGAAGGAAGCACCCAAGTTCAAGTAAGTTCAAGTTTACAAGTTGCTCACATATGTTGCAGAGGTGCT
AGGGCTACAAGG
AGGGTTGGAGTGGACACAGCCAACCCAGAACCACTA
mm4_intron_Rbms3_chr9_116774963 ACTGATGGGTGGAGGAAGGTAGAAGATGAGCTAAGGTGAATGGGATGAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNACTTCCTGGATATGTTCTGATCATGTTGCTGGTGTTAGGAC
mm3_intron_Gm53_chr11_96252161 CCTTGTTCCACCGCATTTCCATCTACTCCAGTTCTTCCTGCTCACAAGCCTAGACTGTAATAGGACCTAAGGCGACAGAG
GTGATCTGCCCTAAATGGCCTCCCTGGTG
mm4_intron_Ttc7_chr17_87358466 AACACTGGTGCGCAGTGATACTTCCATGTCGCCTGGCCCCCTCTTCCAGCACAGGTAGACTCCATAATGAGAAAAGGCTA
GCACTTGACACTGCAAGGCTTCCT
mm4_intergenic_Dock5|Nefm/Nefl_chr14_68021123 TGTCTTTTGATTCCGGCCCACTGGAGCCTAGAAGCAAGAGCCTGCAGAGGAGGCAAGGCCACAGGGAACTGCAGGAGGCA
TTATGAAGAGGGTGGCAGAAGC
mm4_intergenic_Krt33b|Krt34_chr11_100033384 CTGACCCTCCTCTCCCTTGATCTGTTGACTAACCCCCTCAGTGAAACTCTTATCATCGTTTGGCTTCCATGTCCCCTCAG
CTGCTCCTC
CATTGTCCTCTCCAGTTGCTGTACCCAGG
mm4_intergenic_Cacna2d2|Gm17141/Cacna2d2_chr9_107402895 TTGCTGCTAATGGCCCTGATGTATTCTGTCTCTCTTTGTTGCCCCACACTCCAGCTATGGGGTTCCCCCCCTTCTCGTCT
TACCTCCTCTTA
CCACTCTGTACCTAACCTGGCCTGC
mm4_exon_Ccdc113_chr8_95551295 CGGGCAATGGAGATCTACGTCAATCTGGACAAGGAGATCTTGCTGAGAAACGAGCTACTTGGGAAAATTGAAAAAGAAAC
CATACAAGCAGAGGAGGTAAGAGGACACGGTGTGCCAGCCTCCTTCTGTCCACAA
mm4_intron_St3gal3_chr4_117949840 GCTAGTCAGGCCACATGTGTCTTAGTTTCCTTTGTGACCTGTGTACCCTGTCCCCTTAGCTGCTGTGCTCTGGTGGCTTC
CCTGACAGCCCTCAAA
mm4_intron_Tox_chr4_6766911 ACAGTCACTCACACTCTGCCGCAACGATGATGGTCTCATTGCTTTCCTTTGTTTATGTGATGTCTTTCAAAATGCAGTGT
GAAGAGGGTCTGAGGGGACATGGCATTTCCTAGGTAGGCAGAGGCAG
mm4_intron_Dnajb13_chr7_100512333 AGAAGTGGGAGAGGGAAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNACACAGAGAGCAGGGGCTGGGGACAAAACATCA
mm4_exon_Gfod2_chr8_105714559 GAGGTGGGAAGTTGTCAGGGGTCTAGATCTAGACTCTGAGAGGTGCCCTCAAAGTCCCTGTGTCTTCATAATAGTAGTCC
CGCCCCATGTCTACTTACCCCCTCTTCCAGCACTGGGAGGCGATCTCTGAGCACGAACA
mm3_intergenic_Mogat1|Acsl3/Utp14b_chr1_78621178 TGCGTGGGGTTCATAATGGTTCTTAACCTCATTCCCTTGCAGTAAAAAGCTGTGACTCATTCCACAGACTGCAAGTGGAG
CTAAGGGGACACGG
GGAGACCGAGGAAGCAGGCCTATGAG
mm4_intergenic_Gm24478|Gm14255_chr2_162818664 CCTGACACCCTGCTGTCATTTTCTTGCCCTTGCCCCCTTATCTCCTCATCTCTCCTCATTCCATACTCTAATGCACTTTC
TCTTTCCTTTCTTGCCTTTCTCCTATTGGTTAAAACTCCTTCCCTGCAGGAGAAGCAATG
mm4_intergenic_Ncoa1|Gm3625_chr12_4499942 AATGGGGCCAAAGGATCTGGGCAAAGAGCAGAAATGGGCAGGTGCTGGGCAGAGGAACAGGAGCTAAGGCCTCTGGGACA
GTGCAAGCTGTTTCCAGTGACAGGTGGG
mm4_intron_Hmbox1_chr14_64944040 TGCATGAAGAGCCAACAGGGGAAGAGGAATGAAGGGGACAGGGCTCCCTGCTACTTTTGCAGCTCCTTGTGAAAGAGATG
ATACAGCAAAAGCGCTCTCAGGGCTTATG
mm4_exon_Cxadr_chr16_78338506 TCACTGTGGCTTTCTACCCTTAGAGTCCCCCTCTCGCCTTCTCTCCTCTGCTTTTCTTGATGTCACTTAAATAATACCAC
TGTTTATTAGGATTTAAAAGAAAGTCACACCCTCAGTACGCTTTGACCGTGCTTCT
mm3_intergenic_2410076I21Rik|Hcn4_chr9_58818446 CATGCCCCAGTAGAATCCCCAGGGAACAATGGGTGCCCAGGATGGGAGAAAAGCTGGTAGATGAGGAGCTAAGGCTCCAA
GG
CTGGCCTGTGGGTGCTAGGAAGAGGGGAT
mm4_intergenic_Gas6|1700029H14Rik_chr8_13529668 CAGCTCGTGGATGGACAGAGGGAAGTGGAGCTCGGGCGTCAGGGAGACGTAACCTGAGCCTGAGAGGAGGCGAGGCCTCA
GCACTCCTTGGACAG
mm4_intergenic_Cd72|Sit1_chr4_43455045 CCCAGGACATCACCCCAATCACTACTCCAGGTTGCAGCTCCGTCCTCAACCATCAGATTTGGGGTTCCTACCATGGAGCC
TTAGCTCCTCATT
CCTGTGGGTTACCAGGAGAGAAGGTTCATGAGCCCCATTCTGCA
mm4_intergenic_1700021F07Rik|Ankrd60_chr2_173541190 AACCACAAGTGCTGGGCATAGGTAAGGGGGGAAGAGGAGGGAAGGAGCCAAGGGAAAGGAGGAGCCAAAGGGG
mm4_intron_Plac1_chrX_53213557 AGGGCTCATCTACCCAGTGACTTCTTCAAATCTTGCTGTAGCACTTTGTCGCCTTAGCTCCTAGGTGATCAAGTTGGCAT
GGTGGC
mm3_intron_Cacna1c_chr6_118639891 AGTTGTCTGGCTTCCCAACCGTCTCCTTCTAGAGTAAACTACATGAAAGGCCGTCCTAGGGCTATTATTCTTAGAAGAGG
AGGTCAGGCTACAGGG
AAAGGGGACTCTAAGTTCCCAGGCATCTCTGC
mm3_intron_Igsf9b_chr9_27330408 ATCATTGGTGTGGGCAGAGGGGAGGAGAGAAGAGGAGCCAAGGGCACACGGGGAAGAAGAGAGTGTGATTTTTGTCTTAT
ATGTTAGGCACCCTGGGGCGTTTGTGTAAAATCAAGTTGTCTCCCATAGGCAGCA
mm4_intergenic_Tff3|Tff2_chr17_31135724 CTGGCAGAGGAAACACCACTGTGATAAGAGAAGCTAAGTGGACAAGGATAGTAAGGTGACAGATTTGCAGAGGTAGTCAG
GAAGCC
mm4_exon_A830092H15Rik_chr3_26331751 GATGAAGGGATGCCAGGGTCAGGGATGCACCCGAAGGAGAAGAGAGGCTAAGGCGAGCCGGGGCAGGTGGGGAGTCGCGG
GCTGCGGGAGAGGGCGTGTGCGAGCGAGCCCCCCGAGAAAGATGCATGTACTCGGCCAC
mm4_intron_Myo1e_chr9_70324241 TGCATGGCCACTCTGAACTCCACATCCTGGGATGAGAAGTGTAGAAGAGCTAAGGCGAGCAGGAATCCTGTGGCTGTGGT
CTA
mm4_intron_Plxdc1_chr11_97930649 TTGTGTGGCCCTTAGTGACCTACTTAGCCTCTTAGCTGCCTCGTATCATGTAAGACACTGGCTTTTCCTTTTCACCTTCC
CTCCTCTTC
CGGGAAACTTAATAACGCACAAGTCTCTCAGCT
mm4_intron_Slc7a1_chr5_148383645 ACATCAAGGCCAAAGCTGGAACTCTGTCACTCTGACGCCAGAACCCCATGTCCCCTTACGTCCTCCTCACCAGGTAGCTC
CAAGTCAAAGGTGACCTCACACCTTCCTGTTGGCT
mm4_intergenic_AC147224.1|Cdh12_chr15_21008356 TCAGTGTCTGATGTGAGCTACTAGAGTAGCCATGTCCCTCTCTCCTTCCCTCCTCTTCCTTCTATTGCTCTCCTTGTTCC
TATTCTTCTTTTTCCTTAGAACAAATAAGGGTACCAACTTCATCAAGGCTCCA
mm4_exon_Abhd2_chr7_79363190 GCATCTCTGAGGCTGCTTCACTCACTTGTGAATCATTCTCTCCATCATTGTTAAGTGTGTCTGAAGTTGCACGTAGAAGG
GAGTGAACCCTGACCCCTAAGCTCATCTTCATCTTAAGAGAGAGAAGGCGCGTG
mm4_intron_Nrp1_chr8_128421861 CAGGAGCTGAGACGAAGGTCTGTCCTGCCTGGCAGCCTTAGCCCCTCTTCACCTTTCCCTCGGTCACCTCAGACCACCCT
GAT
mm4_intergenic_Gm23708|G6pd2_chr5_61307125 GGCCTACACTTTGATCAGCGTGAAGCTGTCTTTTCCTCATCAACCCTTGGCCCCCTATCTCCTCTTCCTTTTCATTAACA
CATGCTATGATCCTTGCCT
mm3_exon_Tenm1_chrX_42657541 TGCTAACAGAGTTTCCTGAAGGAAATATTCGCCTTACAAAATTGAAGTCTCCAACATACACACTACCATCAGGACCAGAA
GCTAAGGCGACAGGA
GCAAAGAGTTTGTTGTTGTGGGCTGGACCATT
mm4_intergenic_n-R5s209|Gm7846_chr1_27020969 TGCATCACTAAAGGCAGTTGTATCTTCCTTTCCATCATTTCATGCCTTGTGCCCTTAGTTCATCTTCTTGGGGTCTGACT
TTGTATCTGCTATCTTCTGTGGATCCCTCCATCCCTT
mm4_intergenic_Chchd2|2410018M08Rik_chr5_129887473 GAAAAGCTCGGGCGCTAAAGGTTATTGGACAAAGGAGATATCAGTAATGGGCAGAGCCTGGACTCAGCGTCCTAGGCCCG
GAAGTTACGAAGTTGGCGAGGAGGAGCTAAGGCTAACCGGAAGTTCCTGGGGTTGCACTC
mm4_intron_Aire_chr10_78031719 GCCAGCACCCTACATGGAAACAAGCTCTTCTAGGAAGAGCAGCTATGGGGGCAGGGTAACACTGGTCTGCTGGTGCTGCC
TACTAGGGGCAGCAAAGAATCTGTGAGCACAGCCCAGACTAG
mm4_intron_Muc6_chr7_141648539 GGTGTCTAGGGCTGCATCTGCTGCCACCATCTCCCCTCATCTCCTCTTCAGATGCCCACTGGACCCACCTTGTAGAAGGG
CTGGGGGTTCACCACACTGTGGCACTTCTCAAACACACTGCCCTTCTTCAGG
mm4_exon_Tln1_chr4_43554939 CTGAAAGGGCGATGACCCATGCGTGTCAACCCTCAAACTTTGGAAGAGGAAATAAGGGGAGAAGGCTTGGGGTGCAGACA
GGTCCACGGGGTCACGGTGGTAAGGGGGAGTCCCAAGCCATAACAGGGT
mm4_exon_Smg7_chr1_152846130 CACCAAGGCCTCAGACAGAAGAATGTGTATTTACCTGGTCGGCTGGGAAGAGGAGGGAAGGCTCCAGGGTGATGGATGGG
GATGAACTGAGAATTACTCGTTTGACTTGGAGTTTGAGTGACAGGCG
mm4_intergenic_Kif2b|Gm11498_chr11_91748566 GCAATCCTTCCTCAGGCTGTCTACAAGAACATTTGTGTTGTGAATAGTCATACTGTTGACCAAGTCAGACCCTTCTCACA
TTAGCTGCTCTTC
CTTCCTATCAGCAACCTGAGGACC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.