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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314241_F | TCGTCGGCAGCGTCAGAAATCGAGGTGAGCTGCA | 59.3 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314241_R | GTCTCGTGGGCTCGGGAAGGGAACTGTGGGTGGAG | 59.9 | 1.00 |
| mm4_intergenic_Gm14094|U6_chr2_133213786_F | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intergenic_Gm14094|U6_chr2_133213786_R | Primer3: not found at this Tm | N.d. | 0.50 |
| mm4_intergenic_Rab11b-ps2|2700081O15Rik_chr19_7406513_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Rab11b-ps2|2700081O15Rik_chr19_7406513_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intron_Vps53_chr11_76083221_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Vps53_chr11_76083221_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Tmem108_chr9_103727012_F | TCGTCGGCAGCGTCCGCTCACGTTGGCATTTCAA | 60.0 | 0.21 |
| mm4_intron_Tmem108_chr9_103727012_R | GTCTCGTGGGCTCGGACTGGGCCAGTTCTTTCCAA | 59.4 | 0.21 |
| mm4_exon_Hoxb1_chr11_96366821_F | TCGTCGGCAGCGTCCTGACGTGGCTTTTCTCCCT | 59.9 | 0.19 |
| mm4_exon_Hoxb1_chr11_96366821_R | GTCTCGTGGGCTCGGTGGTGAAGTTTGTGCGGAGA | 59.8 | 0.19 |
| mm4_intron_Ctnnd2_chr15_30895056_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Ctnnd2_chr15_30895056_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm23987|Fam135b_chr15_69810105_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm23987|Fam135b_chr15_69810105_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Mei4_chr9_81955470_F | TCGTCGGCAGCGTCTGAGAACCAGGACCACTCATG | 59.3 | 0.08 |
| mm4_intron_Mei4_chr9_81955470_R | GTCTCGTGGGCTCGGAGGATAAATGGGAAAAGGGGCT | 59.4 | 0.08 |
| mm4_intergenic_Stx11|Sf3b5_chr10_12996594_F | TCGTCGGCAGCGTCGGGTACACGTGTGTTAAACGTG | 60.0 | 0.06 |
| mm4_intergenic_Stx11|Sf3b5_chr10_12996594_R | GTCTCGTGGGCTCGGGGTGCAATCAGCCCCTTTTC | 59.7 | 0.06 |
| mm4_intron_Adamtsl4_chr3_95687711_F | TCGTCGGCAGCGTCAGGGAGGTCCTGGGTATGTT | 59.5 | 0.05 |
| mm4_intron_Adamtsl4_chr3_95687711_R | GTCTCGTGGGCTCGGCCTAAGGCGAATTCCTGGGG | 60.1 | 0.05 |
| mm4_exon_Chd7_chr4_8866638_F | TCGTCGGCAGCGTCGAGGTTGCACAGGGTGAAGA | 59.8 | 0.05 |
| mm4_exon_Chd7_chr4_8866638_R | GTCTCGTGGGCTCGGGGACTACCACCTGTCAACAGT | 59.3 | 0.05 |
| mm4_intron_Magi2_chr5_20669378_F | TCGTCGGCAGCGTCAGTGCAGAGTTAGCACAGGG | 59.6 | 0.04 |
| mm4_intron_Magi2_chr5_20669378_R | GTCTCGTGGGCTCGGTGATGGTCTAGCAGGCTCCT | 60.0 | 0.04 |
| mm4_intron_Pcnt_chr10_76425171_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pcnt_chr10_76425171_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Ttyh3_chr5_140648704_F | TCGTCGGCAGCGTCGAAAGGGGATGGGGAAGCTC | 60.1 | 0.03 |
| mm4_exon_Ttyh3_chr5_140648704_R | GTCTCGTGGGCTCGGACCAGCAGCCAATTCCGG | 60.3 | 0.03 |
| mm4_intron_Plcl2_chr17_50587591_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Plcl2_chr17_50587591_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Mb21d2|Hrasls_chr16_29123694_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Mb21d2|Hrasls_chr16_29123694_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ccnd1|Tpcn2_chr7_144941645_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Ccnd1|Tpcn2_chr7_144941645_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36490725_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36490725_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm14303|Tfap2c_chr2_172524256_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm14303|Tfap2c_chr2_172524256_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22610|Dpp10_chr1_123276430_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22610|Dpp10_chr1_123276430_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Lphn3|Mir1187_chr5_81913051_F | TCGTCGGCAGCGTCTTGTCCACTTGTGCCATCCA | 59.8 | 0.02 |
| mm4_intergenic_Lphn3|Mir1187_chr5_81913051_R | GTCTCGTGGGCTCGGCACGGGCTGTGTTGAGATGA | 60.3 | 0.02 |
| mm4_intergenic_Fam173b|Tas2r119_chr15_31906936_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Fam173b|Tas2r119_chr15_31906936_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Tmem65_chr15_58823000_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Tmem65_chr15_58823000_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Dpysl4_chr7_139092991_F | TCGTCGGCAGCGTCCTGCCTTCAGTCCCCAATGG | 60.6 | 0.02 |
| mm4_exon_Dpysl4_chr7_139092991_R | GTCTCGTGGGCTCGGAAGAGCAAGCCTGAGCCTTC | 60.3 | 0.02 |
| mm4_intergenic_Gm14026|AI847159_chr2_129172348_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm14026|AI847159_chr2_129172348_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Slc35f3_chr8_126341192_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Slc35f3_chr8_126341192_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm25759|Gm15338_chr7_124182945_F | TCGTCGGCAGCGTCTCGATCCTTAGTACTTGCTGCA | 59.2 | 0.01 |
| mm4_intergenic_Gm25759|Gm15338_chr7_124182945_R | GTCTCGTGGGCTCGGATGTACAGATGCGAGGCCAG | 59.8 | 0.01 |
| mm4_intron_Tubgcp2_chr7_140036003_F | TCGTCGGCAGCGTCCCTGTCTCTACTGCAGCAGG | 59.8 | 0.01 |
| mm4_intron_Tubgcp2_chr7_140036003_R | GTCTCGTGGGCTCGGGACTCCTCTCTTGACAGCCC | 59.4 | 0.01 |
| mm4_intron_Iffo2_chr4_139536736_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Iffo2_chr4_139536736_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Spink10_chr18_62548965_F | TCGTCGGCAGCGTCTGGTTCCTTGCGGCTCTTC | 60.3 | 0.01 |
| mm4_exon_Spink10_chr18_62548965_R | GTCTCGTGGGCTCGGACACCCCGAAATCAAGGGTC | 59.9 | 0.01 |
| mm4_intergenic_Igkv3-9|Igkv3-7_chr6_70607463_F | TCGTCGGCAGCGTCGGAGACAGACACACTCCTGC | 60.0 | 0.00 |
| mm4_intergenic_Igkv3-9|Igkv3-7_chr6_70607463_R | GTCTCGTGGGCTCGGGGCCACAGAGGTTGCTCATA | 59.7 | 0.00 |
| mm4_intergenic_Igkv3-7|Igkv3-5_chr6_70642431_F | TCGTCGGCAGCGTCGGCATCCTCTCTTCCAGCTC | 59.8 | 0.00 |
| mm4_intergenic_Igkv3-7|Igkv3-5_chr6_70642431_R | GTCTCGTGGGCTCGGGCTCCCAACACTTCTCTGCA | 60.2 | 0.00 |
| mm4_intergenic_Igkv3-12|Igkv3-10_chr6_70553300_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Igkv3-12|Igkv3-10_chr6_70553300_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Igkv3-9_chr6_70588215_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Igkv3-9_chr6_70588215_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Igkv3-5_chr6_70663324_F | TCGTCGGCAGCGTCGGAGACAGACACACTCCTGC | 60.0 | 0.00 |
| mm4_exon_Igkv3-5_chr6_70663324_R | GTCTCGTGGGCTCGGTCTCAACACTTCTCGGCACC | 59.9 | 0.00 |
| mm4_exon_Igkv3-4_chr6_70671815_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Igkv3-4_chr6_70671815_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Igkv3-3_chr6_70686972_F | TCGTCGGCAGCGTCGCCAGGAGCCTAAGAAGCAT | 59.8 | 0.00 |
| mm4_exon_Igkv3-3_chr6_70686972_R | GTCTCGTGGGCTCGGGCATCCAACACTTCTCTGCAC | 59.8 | 0.00 |
| mm4_exon_Igkv3-1_chr6_70703604_F | TCGTCGGCAGCGTCGCCCAAGAAGCATCCTCTCA | 59.7 | 0.00 |
| mm4_exon_Igkv3-1_chr6_70703604_R | GTCTCGTGGGCTCGGGGCTACAGAGGTTGCTCTCA | 59.1 | 0.00 |
| mm4_exon_Igkv3-10_chr6_70572659_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Igkv3-10_chr6_70572659_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Stmnd1|Rbm24_chr13_46302832_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Stmnd1|Rbm24_chr13_46302832_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fam211a|4930557C09Rik_chr11_62626549_F | TCGTCGGCAGCGTCCGGTCTATGTGGGCACAGAT | 59.5 | 0.00 |
| mm4_intergenic_Fam211a|4930557C09Rik_chr11_62626549_R | GTCTCGTGGGCTCGGTGGTGCGAGCTCTCTCTAGT | 60.0 | 0.00 |
| mm4_intergenic_Olfr161|Mefv_chr16_3635705_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Olfr161|Mefv_chr16_3635705_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Camk2b_chr11_5995624_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Camk2b_chr11_5995624_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp746_chr6_48086251_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp746_chr6_48086251_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nfkb1_chr3_135593227_F | TCGTCGGCAGCGTCCCCAGGCAGAGCAGAAAGAA | 59.9 | 0.00 |
| mm4_intron_Nfkb1_chr3_135593227_R | GTCTCGTGGGCTCGGCTGAGCACCAAGAAGGCAGA | 59.9 | 0.00 |
| mm4_intron_Kctd8_chr5_69191684_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Kctd8_chr5_69191684_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cabin1_chr10_75712731_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cabin1_chr10_75712731_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_9030617O03Rik_chr12_100872948_F | TCGTCGGCAGCGTCCTTCCTGGGCATCCATCCC | 59.8 | 0.00 |
| mm4_intron_9030617O03Rik_chr12_100872948_R | GTCTCGTGGGCTCGGTCTCCTGAGAACAGACAAGGC | 59.3 | 0.00 |
| mm4_intron_4930588K23Rik_chr16_18607262_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_4930588K23Rik_chr16_18607262_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Uaca|Gm10655_chr9_61264393_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Uaca|Gm10655_chr9_61264393_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Smim3|Dctn4_chr18_60525865_F | TCGTCGGCAGCGTCTTCAAGGGGTCTGTGGCAAG | 60.1 | 0.00 |
| mm4_intergenic_Smim3|Dctn4_chr18_60525865_R | GTCTCGTGGGCTCGGAGCTTCCAGGGCAGAATTCC | 60.0 | 0.00 |
| mm4_intergenic_Gm4994|Gm7058_chrX_131897013_F | TCGTCGGCAGCGTCTGCAGGGTGTCTGGAAATAGG | 59.7 | 0.00 |
| mm4_intergenic_Gm4994|Gm7058_chrX_131897013_R | GTCTCGTGGGCTCGGTCTACACTGTAGGCCAGGCT | 59.9 | 0.00 |
| mm4_intergenic_Gm11411|Gm11412_chr4_81711988_F | TCGTCGGCAGCGTCATGCGTTGTTTTCCACCAGC | 59.9 | 0.00 |
| mm4_intergenic_Gm11411|Gm11412_chr4_81711988_R | GTCTCGTGGGCTCGGAGAGAACAAGTGCTGGGCTC | 59.9 | 0.00 |
| mm4_intergenic_Fignl2|Ankrd33_chr15_101114801_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Fignl2|Ankrd33_chr15_101114801_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314241 |
AGAAATCGAGGTGAGCTGCACCCCAGACTGCGCCACCGGGAACGCCGAGTACCAGCACAGCAAAGGTAGCCACCTTGCCC CTCCGCTCCCCGGTCCGCCCACTCCACCCACAGTTCCCTTC |
| mm4_intron_Tmem108_chr9_103727012 |
CGCTCACGTTGGCATTTCAATTTTCACTTCTAAACGATATTTCTGTTCCTGGAATGCCGCGTACAAACACTCGTAACTAC ATGAGTGCCATATGGACAGTTATTTTTTTTTTAATTTGGAAAGAACTGGCCCAGT |
| mm4_exon_Hoxb1_chr11_96366821 |
CTGACGTGGCTTTTCTCCCTGCCCCGCCCCTAGCGAAGGTGTCCGAGCTGGGACTGGGCGCTCCCGGCGGTCTCCGCACA AACTTCACCA |
| mm4_intron_Mei4_chr9_81955470 |
TGAGAACCAGGACCACTCATGTAAGGAATAGGTTTATGGAAAGATAAGGGCAGGTATCTTAAGGATCAGTTTCCAGGAAC TCCAAGTACAAGCAAATCAAAACCTAATAAGTTAGCCCCTTTTCCCATTTATCCT |
| mm4_intergenic_Stx11|Sf3b5_chr10_12996594 |
GGGTACACGTGTGTTAAACGTGTGTGTGCTGGTAGTGGGCGCTCAAGGAAAAGCCAGGTTTAGGAGAAAAGGGGCTGATT GCACC |
| mm4_intron_Adamtsl4_chr3_95687711 |
AGGGAGGTCCTGGGTATGTTTTCCAACTTCACTATGTGACTTAATAGGAATCTTGGCACAAGCCCTCGCCCGGGTGATGG GACTCGGAGTCCCAGGGACCAGACCCCCTCCCCAGGAATTCGCCTTAGG |
| mm4_exon_Chd7_chr4_8866638 |
GAGGTTGCACAGGGTGAAGAGCTGGACTCCCTCGAAGGCGGGGATGAGATAGAAAACACCGGAAACGACGAGTAACAGCA GCAGCAGTTCCACTGAAAGTGTTGAAAACTGTTGACAGGTGGTAGTCC |
| mm4_intron_Magi2_chr5_20669378 |
AGTGCAGAGTTAGCACAGGGCCGTTGCATCTTCTTGATTTATCTGGTGAATTGTGCTGGAACTCGGCAGCCCTGGGGCCT GTAGATGAGAGGCAATGAGGTTCTCAAAGGAGCAGTCAGCAGGAGCCTGCTAGACCATCA |
| mm4_exon_Ttyh3_chr5_140648704 |
GAAAGGGGATGGGGAAGCTCCAGCTCCTTGGGAGGGGCCATGACCCGGGGCCCCTGACCCCGCACCCGGGGGCGCGCGTC ACCTGCTGGTAGTCGGCGTCCTCGGGCCGGAATTGGCTGCTGGT |
| mm4_intergenic_Lphn3|Mir1187_chr5_81913051 |
TTGTCCACTTGTGCCATCCATAAGAAATAACCAGGAACCCCGTGTTCCAGCCCGGCTTGATTTTAAGACAGTATCTGCAC TCCTCTCATCATCTCAACACAGCCCGTG |
| mm4_exon_Dpysl4_chr7_139092991 |
CTGCCTTCAGTCCCCAATGGACCTTCATCAGTCCTGGTCCTGGGAGTTCCTGGGCAGATTCTAGTTGCCCAAGAGTTGGG AAGGCTCAGGCTTGCTCTT |
| mm4_intergenic_Gm25759|Gm15338_chr7_124182945 |
TCGATCCTTAGTACTTGCTGCAGTAGGTATGCCAGGAGCACCCAGTCCCAGCACCTGATACATCTGGCCTCGCATCTGTA CAT |
| mm4_intron_Tubgcp2_chr7_140036003 |
CCTGTCTCTACTGCAGCAGGCCCTGATCCCTTCACCCCTGAACCCCGAGCACCAGCAGCAGATGCAGAAAACCCGCAGGC CAGCACCGCAGTCACCGCGGGGCTGTCAAGAGAGGAGTC |
| mm4_exon_Spink10_chr18_62548965 |
TGGTTCCTTGCGGCTCTTCCGTGGTGCTGGTACTCTGTAATCCTGGATGTACGCTGGCTCCCCTCTTCTTCATTGGCAAA ATGAATAGGTGCAATAAAAATATTTTCCAGCGAAAAACAGGACCCTTGATTTCGGGGTGT |
| mm4_intergenic_Igkv3-9|Igkv3-7_chr6_70607463 |
GGAGACAGACACACTCCTGCTATGGGTGCTGCTGCTCTGGGTTCCAGGTGAGGGTACAGATAAGTGTTATGAGCAACCTC TGTGGCC |
| mm4_intergenic_Igkv3-7|Igkv3-5_chr6_70642431 |
GGCATCCTCTCTTCCAGCTCTCAGACATGGAGTCAGACACACTCCTGCTATGGGTGCTGCTGCTCTGGGTTCCAGGTGAG GATGCAGAGAAGTGTTGGGAGC |
| mm4_exon_Igkv3-5_chr6_70663324 | GGAGACAGACACACTCCTGCTATGGGTGCTGCTGCTCTGGGTTCCAGGTGAGGGTGCCGAGAAGTGTTGAGA |
| mm4_exon_Igkv3-3_chr6_70686972 |
GCCAGGAGCCTAAGAAGCATCCTCTCATCTAGTTCTCAGAGATGGAGTCAGACACACTCCTGCTATGGGTGCTGCTGCTC TGGGTTCCAGGTGAGAGTGCAGAGAAGTGTTGGATGC |
| mm4_exon_Igkv3-1_chr6_70703604 |
GCCCAAGAAGCATCCTCTCATCTAGCTCTCAGAGATGGAGTCAGACACACTCCTGCTATGGGTGCTGCTGCTCTGGGTTC CAGGTGAGGATGCAAAGAAGTGTTGAGAGCAACCTCTGTAGCC |
| mm4_intergenic_Fam211a|4930557C09Rik_chr11_62626549 |
CGGTCTATGTGGGCACAGATTATGTGGTGGTACTGGGGGTTCAAGGGAAATTCATGCCCACAGCTGGTGTCTGGAAGAAA AAAACATTACCAACCCAAGGGACAGAGCCCTAGACTTTCCACTAGAGAGAGCTCGCACCA |
| mm4_intron_Nfkb1_chr3_135593227 |
CCCAGGCAGAGCAGAAAGAAAGACAGCGTGAAGAATAAGAGTCTGCAGTCAGGCTGCGGTGCTGTGACTCAGCTTTCCGG GACACTGGGAGTGGGGCTTGATCATCTTTGTAGTCTGCCTTCTTGGTGCTCAG |
| mm4_intron_9030617O03Rik_chr12_100872948 |
CTTCCTGGGCATCCATCCCAGGGGCTGCTGGTGCTCTGCCTTCCAGGACCCGGTTTTAAAACTTTCCACTTCACATTGTG ATTCCCACTCAAACCTTGTTCTAGCCTTGTCTGTTCTCAGGAGA |
| mm4_intergenic_Smim3|Dctn4_chr18_60525865 |
TTCAAGGGGTCTGTGGCAAGGTCGTCGGAAGAATGTGTTGCTGTAACTCGGCTTTCCAGGATGGAATTCTGCCCTGGAAG CT |
| mm4_intergenic_Gm4994|Gm7058_chrX_131897013 |
TGCAGGGTGTCTGGAAATAGGTTCAAGAGATGAAGTAGCATGTNNNNNNNNNNNNNNNNNNNNNNNNNNNTTCACTAGTG CTGGTTCTTGGCCTACCAGGAGGCTCAGCCTGGCCTACAGTGTAGA |
| mm4_intergenic_Gm11411|Gm11412_chr4_81711988 |
ATGCGTTGTTTTCCACCAGCTTGTTCGCAAAGTGTGGTGGAACTCAGCTTTCCTGGGTGTCCAGTAAATACAACCTGCAG AGCAGGAGAGAGCTGAGCCCAGCACTTGTTCTCT |