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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314125_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314125_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm4_intron_Myo3b_chr2_70281250_F | TCGTCGGCAGCGTCACATTTCAGTGCTGGGAGGG | 59.9 | 0.88 |
| mm4_intron_Myo3b_chr2_70281250_R | GTCTCGTGGGCTCGGCGTTGCTTGACTTGGTGTGG | 59.9 | 0.88 |
| mm4_intergenic_4930448K12Rik|Pard3b_chr1_62004696_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_4930448K12Rik|Pard3b_chr1_62004696_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_Gm13814|Gm13814/Gm13817_chr2_92498967_F | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_intergenic_Gm13814|Gm13814/Gm13817_chr2_92498967_R | Primer3: not found at this Tm | N.d. | 0.45 |
| mm4_intergenic_4932435O22Rik|Gm11689_chr11_114558325_F | Primer3: not found at this Tm | N.d. | 0.44 |
| mm4_intergenic_4932435O22Rik|Gm11689_chr11_114558325_R | Primer3: not found at this Tm | N.d. | 0.44 |
| mm3_intron_Itpr1_chr6_108542167_F | TCGTCGGCAGCGTCACTCACGCTGCGGTAATGAA | 60.0 | 0.42 |
| mm3_intron_Itpr1_chr6_108542167_R | GTCTCGTGGGCTCGGAAAGCAGTGACTCTGAGGGG | 59.3 | 0.42 |
| mm4_intron_Fam211b_chr10_75558762_F | TCGTCGGCAGCGTCGCAATATGCCTGGCTGGGTA | 60.1 | 0.42 |
| mm4_intron_Fam211b_chr10_75558762_R | GTCTCGTGGGCTCGGCCAGACACAAGCCTCCTTCC | 60.3 | 0.42 |
| mm4_intergenic_Dnajc24|Gm22002_chr2_105987114_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Dnajc24|Gm22002_chr2_105987114_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Csmd2|Hmgb4_chr4_128225546_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Csmd2|Hmgb4_chr4_128225546_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm2_intergenic_Sufu|Trim8_chr19_46501164_F | TCGTCGGCAGCGTCAAAAGCGAGCTACAGGACCC | 60.0 | 0.38 |
| mm2_intergenic_Sufu|Trim8_chr19_46501164_R | GTCTCGTGGGCTCGGCAGCCATCTTGAGCTCCCAG | 60.4 | 0.38 |
| mm3_intergenic_1700003M07Rik|Bai2_chr4_129982423_F | TCGTCGGCAGCGTCGGATGGTGTCTCTTCCCAGG | 59.4 | 0.38 |
| mm3_intergenic_1700003M07Rik|Bai2_chr4_129982423_R | GTCTCGTGGGCTCGGCTTCTCTGAACAGCCCAGGC | 60.6 | 0.38 |
| mm4_intergenic_Gm10238|Ly6f_chr15_75238817_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intergenic_Gm10238|Ly6f_chr15_75238817_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Kmo_chr1_175655485_F | Primer3: not found at this Tm | N.d. | 0.36 |
| mm4_intron_Kmo_chr1_175655485_R | Primer3: not found at this Tm | N.d. | 0.36 |
| mm3_intergenic_Gm12341|Abr_chr11_76570961_F | TCGTCGGCAGCGTCACCATGGCCGCTAACACTTG | 60.9 | 0.33 |
| mm3_intergenic_Gm12341|Abr_chr11_76570961_R | GTCTCGTGGGCTCGGTCTACAGCAGTACGTGTGCC | 59.7 | 0.33 |
| mm4_intergenic_Gm5607|D630011A20Rik_chr8_12465826_F | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intergenic_Gm5607|D630011A20Rik_chr8_12465826_R | Primer3: not found at this Tm | N.d. | 0.33 |
| mm4_intron_Ank3_chr10_69619773_F | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Ank3_chr10_69619773_R | Primer3: not found at this Tm | N.d. | 0.25 |
| mm4_intron_Snx29_chr16_11588605_F | TCGTCGGCAGCGTCAAGCTTTGGGGTGAGAGAGC | 59.9 | 0.24 |
| mm4_intron_Snx29_chr16_11588605_R | GTCTCGTGGGCTCGGTCCCTCTGTGCTAGGACCAG | 60.3 | 0.24 |
| mm4_intron_Gatsl2_chr5_134107539_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Gatsl2_chr5_134107539_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Emid1_chr11_5106908_F | TCGTCGGCAGCGTCGGGTAGTTGGTTGGGTGTCC | 60.2 | 0.22 |
| mm4_exon_Emid1_chr11_5106908_R | GTCTCGTGGGCTCGGTCCTTATTCCCTCCGGCAGA | 60.0 | 0.22 |
| mm4_intergenic_Ppp1r37|Gemin7_chr7_19563861_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Ppp1r37|Gemin7_chr7_19563861_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm22594|1110002J07Rik_chr10_66702610_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Gm22594|1110002J07Rik_chr10_66702610_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Stk32b_chr5_37710188_F | TCGTCGGCAGCGTCAGCTGACTCTCCTTTGCTGT | 58.9 | 0.22 |
| mm4_intron_Stk32b_chr5_37710188_R | GTCTCGTGGGCTCGGCTTTGAGCCCTGGACAGTGG | 60.6 | 0.22 |
| mm4_intron_Pdia5_chr16_35401463_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Pdia5_chr16_35401463_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_exon_Ccne2_chr4_11191343_F | TCGTCGGCAGCGTCCGTCCTCTCCTGCCATTGG | 60.1 | 0.20 |
| mm4_exon_Ccne2_chr4_11191343_R | GTCTCGTGGGCTCGGGTTCGGCTCCGCGTTTTG | 60.1 | 0.20 |
| mm4_intron_Nr1h4_chr10_89481858_F | TCGTCGGCAGCGTCCTATCGAGAGTTGGTGGAGCC | 59.9 | 0.19 |
| mm4_intron_Nr1h4_chr10_89481858_R | GTCTCGTGGGCTCGGAAGCTTCCAGGTCTCATGGC | 60.0 | 0.19 |
| mm4_intron_Mapk12_chr15_89135076_F | TCGTCGGCAGCGTCAGTTCTGTCCCTTTGCCTCG | 59.9 | 0.18 |
| mm4_intron_Mapk12_chr15_89135076_R | GTCTCGTGGGCTCGGTATCGCTGGGAGGGGAAGAT | 59.8 | 0.18 |
| mm4_intergenic_Stx8|C78197_chr11_68191235_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Stx8|C78197_chr11_68191235_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intron_Calb2_chr8_110160431_F | TCGTCGGCAGCGTCCAGGTCCAGAAGAAGGGCTG | 60.0 | 0.17 |
| mm4_intron_Calb2_chr8_110160431_R | GTCTCGTGGGCTCGGAACGGCTTCTGTATCCCAGC | 60.1 | 0.17 |
| mm3_exon_Irs3_chr5_137645480_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_exon_Irs3_chr5_137645480_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Fzd3_chr14_65261756_F | TCGTCGGCAGCGTCCCCGCATCTCAGTGCCTTTA | 60.1 | 0.16 |
| mm4_intron_Fzd3_chr14_65261756_R | GTCTCGTGGGCTCGGCAGTTTGCAAAGTCGGAGGC | 60.0 | 0.16 |
| mm4_intergenic_Sertad2|Aftph_chr11_20673797_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Sertad2|Aftph_chr11_20673797_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Chst8|Pepd_chr7_34815191_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Chst8|Pepd_chr7_34815191_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Cradd|Socs2_chr10_95357873_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Cradd|Socs2_chr10_95357873_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Hs1bp3|Rhob_chr12_8453572_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Hs1bp3|Rhob_chr12_8453572_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_A930031H19Rik|Gm25261_chr4_131121384_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_A930031H19Rik|Gm25261_chr4_131121384_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Epm2a|Gm9797_chr10_11467793_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Epm2a|Gm9797_chr10_11467793_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Ankrd34b_chr13_92438922_F | TCGTCGGCAGCGTCTGAGACGGACCTGACACTCT | 59.8 | 0.12 |
| mm4_exon_Ankrd34b_chr13_92438922_R | GTCTCGTGGGCTCGGCTGGAGCCTGGGATAGCTTG | 59.8 | 0.12 |
| mm4_intron_Camta2_chr11_70687560_F | TCGTCGGCAGCGTCGGTGGTCTAAGACTGTCGCC | 60.1 | 0.12 |
| mm4_intron_Camta2_chr11_70687560_R | GTCTCGTGGGCTCGGGCTCAGCTGCTTCTCCAGAA | 60.0 | 0.12 |
| mm4_intergenic_Gm22004|Vash1_chr12_86666646_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm22004|Vash1_chr12_86666646_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Cradd|Socs2_chr10_95357816_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Cradd|Socs2_chr10_95357816_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Tal1|Pdzk1ip1_chr4_115086591_F | TCGTCGGCAGCGTCACGCTCCTCCCTTAATGTGC | 60.1 | 0.11 |
| mm4_intergenic_Tal1|Pdzk1ip1_chr4_115086591_R | GTCTCGTGGGCTCGGGAAATACCCCAGCCTCTGCC | 60.4 | 0.11 |
| mm4_exon_Phtf2_chr5_20882117_F | TCGTCGGCAGCGTCCTGGAGCCCAAAGAGCTACA | 59.3 | 0.11 |
| mm4_exon_Phtf2_chr5_20882117_R | GTCTCGTGGGCTCGGGACTCCGTGGTCATGCGAG | 60.5 | 0.11 |
| mm4_intron_Rab11fip3_chr17_26024597_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Rab11fip3_chr17_26024597_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm26137|Gm23733_chr3_119911410_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm26137|Gm23733_chr3_119911410_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Nav1|Nav1/Gm4793_chr1_135545127_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Nav1|Nav1/Gm4793_chr1_135545127_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm12371|Gm12372_chr4_37122330_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm12371|Gm12372_chr4_37122330_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Vrk3_chr7_44775574_F | TCGTCGGCAGCGTCGTTCACTTAGCCCCTCACCC | 60.0 | 0.10 |
| mm4_intron_Vrk3_chr7_44775574_R | GTCTCGTGGGCTCGGAGATCACCCAGCTGAATTCCA | 59.0 | 0.10 |
| mm4_intron_Trrap_chr5_144811553_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Trrap_chr5_144811553_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Pgrmc2_chr3_41082539_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_Pgrmc2_chr3_41082539_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_5730508B09Rik|Gm10650_chr3_127970078_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_5730508B09Rik|Gm10650_chr3_127970078_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intron_BC100451_chr11_118339485_F | TCGTCGGCAGCGTCTAACTGTGGTGCCTGTGTGG | 60.1 | 0.09 |
| mm4_intron_BC100451_chr11_118339485_R | GTCTCGTGGGCTCGGTGGATGTTAGTGTCTGAGTCCTG | 59.4 | 0.09 |
| mm4_exon_Ikzf4_chr10_128634262_F | TCGTCGGCAGCGTCAAAGAGGATGCGGCAGTGTT | 60.2 | 0.09 |
| mm4_exon_Ikzf4_chr10_128634262_R | GTCTCGTGGGCTCGGCCTATGCCAAAGAGGACCCC | 59.8 | 0.09 |
| mm4_intron_Stard3_chr11_98374355_F | TCGTCGGCAGCGTCAATCCTCCCCTCTCTCCAGG | 59.7 | 0.09 |
| mm4_intron_Stard3_chr11_98374355_R | GTCTCGTGGGCTCGGTAACACCTTCGCTCAGTCGC | 60.3 | 0.09 |
| mm4_intron_Rab11fip5_chr6_85366732_F | TCGTCGGCAGCGTCCCCACTGCCTTAAGCTCCAA | 59.9 | 0.08 |
| mm4_intron_Rab11fip5_chr6_85366732_R | GTCTCGTGGGCTCGGAATACCTGCCCTTGCTGCTT | 59.9 | 0.08 |
| mm4_intergenic_Fars2|Gm26395_chr13_36577653_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Fars2|Gm26395_chr13_36577653_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_exon_Pld4_chr12_112768857_F | TCGTCGGCAGCGTCCACGGTGACATGGGAAGACA | 59.9 | 0.08 |
| mm4_exon_Pld4_chr12_112768857_R | GTCTCGTGGGCTCGGGGAATGGCCCTAGCAACTCA | 59.7 | 0.08 |
| mm4_intergenic_Nme7|Atp1b1_chr1_164368920_F | TCGTCGGCAGCGTCGTTGCTCTTCTTCCAGTCCCA | 59.9 | 0.08 |
| mm4_intergenic_Nme7|Atp1b1_chr1_164368920_R | GTCTCGTGGGCTCGGACGAGACCAGGGAAAACCAG | 59.6 | 0.08 |
| mm4_intron_Tspan9_chr6_127983565_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Tspan9_chr6_127983565_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Hs3st4/Gm27040|Gm25759_chr7_124125631_F | TCGTCGGCAGCGTCAGGGAAAGAGAATAGCATGCTCT | 59.2 | 0.07 |
| mm4_intergenic_Hs3st4/Gm27040|Gm25759_chr7_124125631_R | GTCTCGTGGGCTCGGACATGAGCAGAGGGCCAAAA | 59.8 | 0.07 |
| mm4_intergenic_Gm4894|Drd2_chr9_49340601_F | TCGTCGGCAGCGTCCCGGGGATCTGAACGGCG | 62.8 | 0.07 |
| mm4_intergenic_Gm4894|Drd2_chr9_49340601_R | GTCTCGTGGGCTCGGGCTCGAGGCTTCCAGCTC | 60.2 | 0.07 |
| mm4_intron_Camta1_chr4_151467488_F | TCGTCGGCAGCGTCTGCTTCGTGCTCTAACCCAG | 60.0 | 0.07 |
| mm4_intron_Camta1_chr4_151467488_R | GTCTCGTGGGCTCGGGTGGGATGTGACAGGTGAGG | 60.0 | 0.07 |
| mm3_intron_Spock1_chr13_57770052_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Spock1_chr13_57770052_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tmem117_chr15_94767090_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Tmem117_chr15_94767090_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Slc6a7_chr18_60996563_F | TCGTCGGCAGCGTCAACCTCCCATTGCCTGTCAG | 59.9 | 0.06 |
| mm4_exon_Slc6a7_chr18_60996563_R | GTCTCGTGGGCTCGGCCACCAAATAGGAGGGCTGG | 60.1 | 0.06 |
| mm3_intron_Ndrg3_chr2_156938745_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intron_Ndrg3_chr2_156938745_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Fcho1|Zfp709_chr8_71838362_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Fcho1|Zfp709_chr8_71838362_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_n-R5s42|Fam19a5_chr15_87624242_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_n-R5s42|Fam19a5_chr15_87624242_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ccbl2_chr3_142719182_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Ccbl2_chr3_142719182_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Gm22302|Uri1_chr7_37887062_F | TCGTCGGCAGCGTCTCCTGGCTAATGTTCTCAGCC | 59.7 | 0.05 |
| mm4_intergenic_Gm22302|Uri1_chr7_37887062_R | GTCTCGTGGGCTCGGCTTCTTTGCGAGCTGAGGGA | 60.0 | 0.05 |
| mm4_intergenic_Gm8321|Inhbb_chr1_119321303_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm8321|Inhbb_chr1_119321303_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Brd3_chr2_27466268_F | TCGTCGGCAGCGTCGGGTGCCAGTTGTATCTGCT | 60.0 | 0.05 |
| mm4_intron_Brd3_chr2_27466268_R | GTCTCGTGGGCTCGGGGTGTGGTGATTCTAGGGCC | 60.1 | 0.05 |
| mm4_intergenic_Gm22673|Gm23059_chr12_59873960_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm22673|Gm23059_chr12_59873960_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Psen2_chr1_180233210_F | TCGTCGGCAGCGTCCGTCCACTCTAAGCCACTGT | 59.3 | 0.04 |
| mm4_exon_Psen2_chr1_180233210_R | GTCTCGTGGGCTCGGCTTGTAGCCACCACTCAGCC | 60.6 | 0.04 |
| mm4_intergenic_Tshz2/Gm26883|Gm26883_chr2_169692823_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tshz2/Gm26883|Gm26883_chr2_169692823_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25945|2810403A07Rik_chr3_88707557_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm25945|2810403A07Rik_chr3_88707557_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Bmp6_chr13_38433898_F | TCGTCGGCAGCGTCGATGCCCTGAGTAGCTGACC | 59.8 | 0.04 |
| mm4_intron_Bmp6_chr13_38433898_R | GTCTCGTGGGCTCGGTGGGCTGTCAACCGAAATCA | 59.8 | 0.04 |
| mm4_intron_Arhgef10_chr8_14911776_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Arhgef10_chr8_14911776_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Sh3rf3_chr10_59119203_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Sh3rf3_chr10_59119203_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Tnfrsf19_chr14_61037562_F | TCGTCGGCAGCGTCAAATGTTCCCTTCTGGCCCG | 60.6 | 0.03 |
| mm4_intron_Tnfrsf19_chr14_61037562_R | GTCTCGTGGGCTCGGCTAGCCCATCCACCCTCTCT | 60.1 | 0.03 |
| mm4_intergenic_Tmx1|Gm24474_chr12_70503326_F | TCGTCGGCAGCGTCAACACCAGGAAACCCAGTCC | 59.8 | 0.03 |
| mm4_intergenic_Tmx1|Gm24474_chr12_70503326_R | GTCTCGTGGGCTCGGACTCCTGGAGGATTCATTGCC | 59.7 | 0.03 |
| mm4_intergenic_Gm23312|Gm6586_chr5_9762338_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm23312|Gm6586_chr5_9762338_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Zfp438|Gm10125_chr18_5428298_F | TCGTCGGCAGCGTCGGAGGGCATGCACAATGGA | 60.3 | 0.03 |
| mm4_intergenic_Zfp438|Gm10125_chr18_5428298_R | GTCTCGTGGGCTCGGTGTTCTGAAGAGGCAGCTGG | 59.9 | 0.03 |
| mm4_intergenic_Gm24404|Nrg3_chr14_38055715_F | TCGTCGGCAGCGTCACAGATGAGGAGACTGGGGA | 59.2 | 0.03 |
| mm4_intergenic_Gm24404|Nrg3_chr14_38055715_R | GTCTCGTGGGCTCGGTCACAAAAGAGAACCTTGATTCCT | 58.3 | 0.03 |
| mm4_intergenic_Baz1a|2700097O09Rik_chr12_55032708_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Baz1a|2700097O09Rik_chr12_55032708_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Galnt18|Gm25787_chr7_111780216_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Galnt18|Gm25787_chr7_111780216_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_exon_Slc27a2/Gm26697_chr2_126553119_F | TCGTCGGCAGCGTCTCCTGCTCTGCTCCTCTCC | 60.3 | 0.02 |
| mm4_exon_Slc27a2/Gm26697_chr2_126553119_R | GTCTCGTGGGCTCGGTGTAGAGCACTGGCAGCATG | 60.3 | 0.02 |
| mm3_intron_Trpm5_chr7_143072825_F | TCGTCGGCAGCGTCGGAGAGAGCCAAGGGAGGTA | 60.0 | 0.02 |
| mm3_intron_Trpm5_chr7_143072825_R | GTCTCGTGGGCTCGGGGGCAATCACATGGGAGGAA | 60.0 | 0.02 |
| mm4_exon_Fam98c/RP23-96N11.4/1110035H17Rik_chr7_29155191_F | TCGTCGGCAGCGTCACTCCCGAAGTAGCTGGCTA | 60.0 | 0.02 |
| mm4_exon_Fam98c/RP23-96N11.4/1110035H17Rik_chr7_29155191_R | GTCTCGTGGGCTCGGTAGCCCTGTGCCATCCTTTG | 60.0 | 0.02 |
| mm4_intergenic_Pros1|Epha3_chr16_62944695_F | TCGTCGGCAGCGTCACACTTGACAATGTCAGCTTACT | 58.2 | 0.02 |
| mm4_intergenic_Pros1|Epha3_chr16_62944695_R | GTCTCGTGGGCTCGGCCCCTAGACACTCCTGACCT | 59.6 | 0.02 |
| mm4_exon_Col23a1_chr11_51290170_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Col23a1_chr11_51290170_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26630280_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm22227|Zfp703_chr8_26630280_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tpsab1|Tpsb2_chr17_25361907_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tpsab1|Tpsb2_chr17_25361907_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Nckap5_chr1_126569881_F | TCGTCGGCAGCGTCTGTGCACTCGGATCAATGGT | 59.6 | 0.01 |
| mm4_intron_Nckap5_chr1_126569881_R | GTCTCGTGGGCTCGGTTCTCTCTGACTCGTCTAGGT | 57.0 | 0.01 |
| mm4_intron_Fam46a_chr9_85326868_F | TCGTCGGCAGCGTCGAGTGGATTTAGGGGAGCGG | 59.8 | 0.01 |
| mm4_intron_Fam46a_chr9_85326868_R | GTCTCGTGGGCTCGGGCTACTATGGGCGTCACGAT | 59.6 | 0.01 |
| mm4_intron_Gm14155_chr2_151830995_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Gm14155_chr2_151830995_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_exon_Kcnc1_chr7_46396968_F | TCGTCGGCAGCGTCACAAGGTGCTGCGCTCAG | 60.6 | 0.01 |
| mm3_exon_Kcnc1_chr7_46396968_R | GTCTCGTGGGCTCGGTCCCTCCTCGTTCCTTCTCC | 60.3 | 0.01 |
| mm4_intron_Zcchc14_chr8_121642447_F | TCGTCGGCAGCGTCCTGAAGGCCCCAGTGTAGC | 60.0 | 0.01 |
| mm4_intron_Zcchc14_chr8_121642447_R | GTCTCGTGGGCTCGGGAATGAGGTGGAAGGCAGCT | 60.0 | 0.01 |
| mm4_intron_Cdhr3_chr12_33046268_F | TCGTCGGCAGCGTCGGAGAAGTGTTGCTGAGGACA | 59.9 | 0.01 |
| mm4_intron_Cdhr3_chr12_33046268_R | GTCTCGTGGGCTCGGTCCTGGAAAAGTGCTGTGGG | 60.1 | 0.01 |
| mm4_intergenic_Scgb1c1|Odf3_chr7_140847603_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Scgb1c1|Odf3_chr7_140847603_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Acat1_chr9_53600336_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Acat1_chr9_53600336_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tbc1d1|Gm25306_chr5_64504049_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tbc1d1|Gm25306_chr5_64504049_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Dnah2_chr11_69429477_F | TCGTCGGCAGCGTCAGAGACAGGTGGCAGTTTGC | 60.5 | 0.01 |
| mm4_exon_Dnah2_chr11_69429477_R | GTCTCGTGGGCTCGGTTTCCCAAGAACCTCCCAGC | 59.8 | 0.01 |
| mm3_intergenic_Nkd1|Snx20_chr8_88616619_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_Nkd1|Snx20_chr8_88616619_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Bahcc1_chr11_120259739_F | TCGTCGGCAGCGTCGGTACCTGTTGCCCTCAGAC | 60.0 | 0.01 |
| mm4_intron_Bahcc1_chr11_120259739_R | GTCTCGTGGGCTCGGAGACCTCAGACGTCTGGACA | 59.8 | 0.01 |
| mm4_intron_Lhx8_chr3_154328307_F | TCGTCGGCAGCGTCGTTCCCAGACCAGCAGGAC | 60.0 | 0.01 |
| mm4_intron_Lhx8_chr3_154328307_R | GTCTCGTGGGCTCGGTGGGGAAGAAGGACTGGTGA | 60.1 | 0.01 |
| mm4_intron_Brd4_chr17_32198321_F | TCGTCGGCAGCGTCCTTCATCCTCTCGGCTCCTG | 59.6 | 0.01 |
| mm4_intron_Brd4_chr17_32198321_R | GTCTCGTGGGCTCGGGCAGAGAAGGAGAAGGAGCG | 60.1 | 0.01 |
| mm4_intron_Apba1_chr19_23878393_F | TCGTCGGCAGCGTCTGTTTCTGGAGAACAAACATGAAGG | 59.9 | 0.00 |
| mm4_intron_Apba1_chr19_23878393_R | GTCTCGTGGGCTCGGCAGGGGTAGGGCCAGATAGT | 60.1 | 0.00 |
| mm4_intron_Opcml_chr9_28882554_F | TCGTCGGCAGCGTCGTGTGTCTGGCCTCTGGATG | 60.3 | 0.00 |
| mm4_intron_Opcml_chr9_28882554_R | GTCTCGTGGGCTCGGGGACTGCAAATACACAAGGCA | 59.1 | 0.00 |
| mm4_intergenic_Sox2ot|Gm20446_chr3_34212143_F | TCGTCGGCAGCGTCGCTGGAACTAGCTCCCCTTG | 60.1 | 0.00 |
| mm4_intergenic_Sox2ot|Gm20446_chr3_34212143_R | GTCTCGTGGGCTCGGAGACTGGGTGCAAGCTACAC | 59.9 | 0.00 |
| mm4_intergenic_Ces5a|Gm26843_chr8_93703333_F | TCGTCGGCAGCGTCCAGAGCACACTCCTTCTGCA | 59.9 | 0.00 |
| mm4_intergenic_Ces5a|Gm26843_chr8_93703333_R | GTCTCGTGGGCTCGGGGCATGTGACAAAGCATGGG | 60.1 | 0.00 |
| mm4_intergenic_Gm23905|Zfp36l2_chr17_84013641_F | TCGTCGGCAGCGTCCTAACCTTGTCAGCCCTGCA | 59.9 | 0.00 |
| mm4_intergenic_Gm23905|Zfp36l2_chr17_84013641_R | GTCTCGTGGGCTCGGCAGAAGGGAAGGGGAAGCAG | 60.0 | 0.00 |
| mm4_intron_Fam198a_chr9_121961738_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Fam198a_chr9_121961738_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_D1Ertd622e|Ppip5k2_chr1_97676990_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_D1Ertd622e|Ppip5k2_chr1_97676990_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Tmem44_chr16_30550300_F | TCGTCGGCAGCGTCGGACTTGAGCTACCTGCAGG | 60.1 | 0.00 |
| mm4_exon_Tmem44_chr16_30550300_R | GTCTCGTGGGCTCGGACACGCTGTAATGGGGACG | 60.0 | 0.00 |
| mm4_exon_Gtf2i_chr5_134238169_F | TCGTCGGCAGCGTCAACCAACCAGGCAGACACAC | 60.7 | 0.00 |
| mm4_exon_Gtf2i_chr5_134238169_R | GTCTCGTGGGCTCGGGCTTGAGCAGGTGGAGGATT | 60.0 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314125 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_intron_Myo3b_chr2_70281250 |
ACATTTCAGTGCTGGGAGGGCCAAGGTGAGCAGTTGATTTGTTCCACGGATGGGGCTGGGAGTCCATGCCACACCAAGTC AAGCAACG |
| mm3_intron_Itpr1_chr6_108542167 |
ACTCACGCTGCGGTAATGAAGCATCTCACCTCCATGGAGGGGACTGGGGGTCAGAAGACAGACTCCCCCCTCAGAGTCAC TGCTTT |
| mm4_intron_Fam211b_chr10_75558762 |
GCAATATGCCTGGCTGGGTACTGCTTGCCAGCTACTCTCCTGCTTATATATTCAATATGGTTCAAGCCACAGATGCCAGC CAAGGAGGGGGTTGGGGATCCCAAAGGAAGGAGGCTTGTGTCTGG |
| mm2_intergenic_Sufu|Trim8_chr19_46501164 | AAAAGCGAGCTACAGGACCCTCAAGCGCGCGCCCGGACCCCCAGTCCCCACCGCGGCCGCTGGGAGCTCAAGATGGCTG |
| mm3_intergenic_1700003M07Rik|Bai2_chr4_129982423 |
GGATGGTGTCTCTTCCCAGGTTATCTCAGCCTCAGGCTCCCTGGAGGGGGGTGGGGGTCCCTCCTGGGGCTAAGATGCAT TTTTCTCACCCCCGCCTGGGCTGTTCAGAGAAG |
| mm3_intergenic_Gm12341|Abr_chr11_76570961 |
ACCATGGCCGCTAACACTTGCCGGTGCCTGGGTGCACCTGGGACCCGGAGGGCAACAGGGCTCCTCGGAGGGTGCTGGGT GTCCCTCTTACACCCCCTGTCCCCCACCCAGGGACCCATGGGCACACGTACTGCTGTAGA |
| mm4_intron_Snx29_chr16_11588605 |
AAGCTTTGGGGTGAGAGAGCCATGGACACCCAAGAATATCTCTGGGACCACTTGTCCCCTCAGTGGGCTGCAGAGCTGTA TCATGATACTGGATAAGACTGAAGCTATGACTGGTCCTAGCACAGAGGGA |
| mm4_exon_Emid1_chr11_5106908 |
GGGTAGTTGGTTGGGTGTCCTCCCCTCTTGCCTCGAAGGAGGCTGGGGGTACCTGTGCCATCAGGGCCTGCCCCGGATCC CAAGTCTGGTTCTGCCGGAGGGAATAAGGA |
| mm4_intron_Stk32b_chr5_37710188 |
AGCTGACTCTCCTTTGCTGTTGTAAACTTTCCCTCACTACCATGGTAACTAGTATACCCTGTGAAGTGGGGGACCCCAAG ATCCCTCTGAGGAGCAGTTTCTCCATCTCCACTGTCCAGGGCTCAAAG |
| mm4_exon_Ccne2_chr4_11191343 |
CGTCCTCTCCTGCCATTGGCCGGCTCCTCCCGGAGGGGTGGGCCCTAGGCTCCCGCTCCCCGTGCCTCTGAGGAGACTTG GGGTTCCCCACCCCAGCCCTCCCAAGGCCTCTGCCAAAACGCGGAGCCGAAC |
| mm4_intron_Nr1h4_chr10_89481858 |
CTATCGAGAGTTGGTGGAGCCCTGAGGAGGGGCCCAGATCTGTGGGATGCCCTCAGATGGGACTGTGGGACCCCAGCTTC TTCCTCGGTCCCTTTTTCTTCCAGGCCATGAGACCTGGAAGCTT |
| mm4_intron_Mapk12_chr15_89135076 |
AGTTCTGTCCCTTTGCCTCGGCCAAGGGTCAAGCAGACCCATAGGGGAAGTAGAAGGGGTCCCCCAACCCCTTCCGTGGT CATCTTCCCCTCCCAGCGATA |
| mm4_intron_Calb2_chr8_110160431 |
CAGGTCCAGAAGAAGGGCTGTCACCACTGAGGGGACAGGTGCTCCCTCCAAGGCCACCACAGCCATCATTTAACCCTTTC ATGTAAGGCTGGGATACAGAAGCCGTT |
| mm4_intron_Fzd3_chr14_65261756 |
CCCGCATCTCAGTGCCTTTATTCCAGAGGCTAGGAATTCACGCCGCATCCCAGGGCAAATCTGCCGACCCCGAGTCCCCA CCGCGGGGGTACCTGGGGAGCGCGCGGCGCTGGGAGCCTCCGACTTTGCAAACTG |
| mm4_exon_Ankrd34b_chr13_92438922 |
TGAGACGGACCTGACACTCTTTGGCTTCAAGGATAAGGAGTTGTGTGGGGGCAGTGATAATACCTGGGACCCAGACTCTC CTCCGAGGAAGCCTGTGATAGCCACTAATGGGCCCAAGCTATCCCAGGCTCCAG |
| mm4_intron_Camta2_chr11_70687560 |
GGTGGTCTAAGACTGTCGCCGGGGGTGGGATGCTCTCCCCAAAGGGGGCAGGGGTCCTGGGAGAACCCCGGAGGTGGGAG GGGGTCCCGCCCGCAAACCCGGATGTTCTGGAGAAGCAGCTGAGC |
| mm4_intergenic_Tal1|Pdzk1ip1_chr4_115086591 |
ACGCTCCTCCCTTAATGTGCTCAAAGTATAGTTCTGGGAGTCTCTGCTTTCCTTTGGGATTTTAGGAAAATTGGGGATGC TGGGGGCCGGGGGGGGGACTGTGGCTCCCAAGCTGGTGGCAGAGGCTGGGGTATTTC |
| mm4_exon_Phtf2_chr5_20882117 |
CTGGAGCCCAAAGAGCTACANNNNNNNNNNNNNNNNNNNNNTGCCTCCACCCTCGCGCCGCTCGCGCCGCCCGCACCGCG CTCCGCCTCCCGGGACGCCCCGCCTCCTCCGCGGCTCCTCGCATGACCACGGAGTC |
| mm4_intron_Vrk3_chr7_44775574 |
GTTCACTTAGCCCCTCACCCCACCCAAGCCTGTGAGGGGACTGGGTGCCCCAGCCCCTGTTATATACCCTTGGTTTTTCA TCAGCTCCTGAAGTCAATTCAAGAGCTGGAATTCAGCTGGGTGATCT |
| mm4_intron_BC100451_chr11_118339485 |
TAACTGTGGTGCCTGTGTGGATTCTTCATGAGGGTCCTCCACTCCCCTCCCTGGGACACCCACAATAGGAGATCTTCTAC AGCCTATATACTCAGGACTCAGACACTAACATCCA |
| mm4_exon_Ikzf4_chr10_128634262 |
AAAGAGGATGCGGCAGTGTTCACACTTAAAGGCCTTCACTGGCTCACCACTCTCACCCACCACCCGAAGCACTTCCTTGG AGGGGCCTGGGGTGCCCCGCAGTAACCCCTCCTGTGGTTTGGGGTCCTCTTTGGCATAGG |
| mm4_intron_Stard3_chr11_98374355 |
AATCCTCCCCTCTCTCCAGGGGCTGGGATCTCCATTCCCCTCTGAGGAGTTGAGATACAGTCTGGTTCTTGGACTCTGTC CAGACTCCCTTGTCTCTCCAATCCCTGGACATTGGCGACTGAGCGAAGGTGTTA |
| mm4_intron_Rab11fip5_chr6_85366732 |
CCCACTGCCTTAAGCTCCAAAGGTCTGCCTGTCAGCTGGCCTCAGAAGGGGCTGGGGGCCCCACACTATAAAAATGCCAG GAGACAAGTCTAACCACCAAGCAGCAAGGGCAGGTATT |
| mm4_exon_Pld4_chr12_112768857 |
CACGGTGACATGGGAAGACAGCAACTACATATAAGTGACCCCCAGAACCCTCCCTGGTCTACCTATGATGAGTTGCTAGG GCCATTCC |
| mm4_intergenic_Nme7|Atp1b1_chr1_164368920 |
GTTGCTCTTCTTCCAGTCCCAAAGGAGTTCACCTCTCAGATGAGGAGGAACCTCCCCTGCAGGGAGCTGGGGGTCCCTGT GGTGCAGAGCCTGCTTTGTAGGTTTCTGGTTTTCCCTGGTCTCGT |
| mm4_intergenic_Hs3st4/Gm27040|Gm25759_chr7_124125631 |
AGGGAAAGAGAATAGCATGCTCTCTCATACAAAAATAGTAGGTGTCCCTCTGAAGGGACAGTGGGTCCCAGGTCTCCAAA AGTATTACCAAAGGAAGTTCCCTTTAGCTTTTGTTGTTTTTTGGCCCTCTGCTCATGT |
| mm4_intergenic_Gm4894|Drd2_chr9_49340601 |
CCGGGGATCTGAACGGCGCGGCGGGGCCGGGAGCCGAGGGACCCGCAGAGGGGACCGGCGGCCCCGGACGGCTGCCGGAG GGGCGGCCGTGCGTGGATGCGGCGGGAGCTGGAAGCCTCGAGC |
| mm4_intron_Camta1_chr4_151467488 |
TGCTTCGTGCTCTAACCCAGCGCGTCTCATCTGGCCAAGTCCATGCCTCCACGGTGGGGCCTGGCGCTCCCACCGACCCT CACCTGTCACATCCCAC |
| mm4_exon_Slc6a7_chr18_60996563 | AACCTCCCATTGCCTGTCAGAATCCCCTGCAGGCCACGGAAGGGACTGTGAGGCCCCGACCAGCCCTCCTATTTGGTGG |
| mm4_intergenic_Gm22302|Uri1_chr7_37887062 |
TCCTGGCTAATGTTCTCAGCCTCTGCTCTCGGGGCTCCAGCGAGCCCCCCTCCCCTCCGAGGTTAACAGGATGAAGGCTG GATAAGGAGCTACTCATTCCCTCAGCTCGCAAAGAAG |
| mm4_intron_Brd3_chr2_27466268 |
GGGTGCCAGTTGTATCTGCTCTGAGACTCCAGGGATCCCCAGTACCCACAGTGGGCCACTTCACAGGCCCTAGAATCACC ACACC |
| mm4_exon_Psen2_chr1_180233210 |
CGTCCACTCTAAGCCACTGTCCATGAACCCCAGAGGGAAGTGGGGGGCCCTGCCCTCCAAAGCCTGCAGGGCTGAGTGGT GGCTACAAG |
| mm4_intron_Bmp6_chr13_38433898 |
GATGCCCTGAGTAGCTGACCACATTCCACCCACTGTGACTCAATAGAAACCCCCAGGCCCCTCGGAGGCAGGCTGGCATA GTTGAAACCCCCCAATGCTGTCTGTTGATTTCGGTTGACAGCCCA |
| mm4_intron_Tnfrsf19_chr14_61037562 |
AAATGTTCCCTTCTGGCCCGCTCCGTTGCAGTTGCGCAGTGGGACGTGGGCTCCAGCTGGGGCCCCCAATGCCCTCCCAG GGACAGGGGACAAGAGAGAGGGTGGATGGGCTAG |
| mm4_intergenic_Tmx1|Gm24474_chr12_70503326 |
AACACCAGGAAACCCAGTCCTGGTCAGGAGTTTCCCTGGCAGGGAACAGGGGGTCCCTGACTCTTTCGGAAGATGTCTTC CCCTCCAAGGGAGTGAAGGATGAAAGGAGAGGCAATGAATCCTCCAGGAGT |
| mm4_intergenic_Zfp438|Gm10125_chr18_5428298 |
GGAGGGCATGCACAATGGAGCCCCGTCGCGGCCCCTCGGAGGACAATGGGGGTCCTCCGGGACCCGCAGTAGGCTGTCCG GGCCAGCCCGAGCTCCAGCTGCCTCTTCAGAACA |
| mm4_intergenic_Gm24404|Nrg3_chr14_38055715 | ACAGATGAGGAGACTGGGGAAGTCCTCTCAGGGGACTGAGGGTCACAGTTCCTAAGGAATCAAGGTTCTCTTTTGTGA |
| mm4_exon_Slc27a2/Gm26697_chr2_126553119 |
TCCTGCTCTGCTCCTCTCCCGCCTGCCGCCGCGCTGCACGCCTCGAGCACTCCCTCGGCCCCGGCGGGGACCGGGGACCC CGCAGCTACCGCCATGCTGCCAGTGCTCTACA |
| mm3_intron_Trpm5_chr7_143072825 | GGAGAGAGCCAAGGGAGGTACTGAGACTTCGGTGGGGACTGGGGGGACCGGGTTGGTTTCCTCCCATGTGATTGCCC |
| mm4_exon_Fam98c/RP23-96N11.4/1110035H17Rik_chr7_29155191 |
ACTCCCGAAGTAGCTGGCTAGCCAGGGTTCCACGCAGGGGTCTGGGGAGCCCCAGGGCTTGAAGAGTTAGCGTCAGTTCC TGAACAACGCCATTCGCCTTCTTTGTCCCTTCTCCAAAGGATGGCACAGGGCTA |
| mm4_intergenic_Pros1|Epha3_chr16_62944695 |
ACACTTGACAATGTCAGCTTACTAGCTCAAGAAACAATAGAAACTTCATCCCCTTTCTCCAAGGAGGGCACTGTGGGTGC CCCAGAGTTTCTCCTCAGAGAATAGAGAAGTTGCAAGGTCAGGAGTGTCTAGGGG |
| mm4_intron_Nckap5_chr1_126569881 |
TGTGCACTCGGATCAATGGTTCCTTACATGTAGGCCCCCCAGTCCCCTGAGTGGCTTAAACAGAACTAAAAAGTTTCCTT TTGATTTATTTTTGGATGAGATAGGAGATTTCTAAATACCTAGACGAGTCAGAGAGAA |
| mm4_intron_Fam46a_chr9_85326868 |
GAGTGGATTTAGGGGAGCGGCAGAGAGGTGTGCGGGTGAGGGGACAGGGGTCCCGCCGAGGGGCCTGGGCTTCCCCTCCC CGATCGTGACGCCCATAGTAGC |
| mm3_exon_Kcnc1_chr7_46396968 |
ACAAGGTGCTGCGCTCAGCGGCCGCCCCGCGGGGCAGCACCCTGGTGCCCCTACCCAGGGGACCCGCCATCCCCTCCGGG AGGCGGGGAGAAGGAACGAGGAGGGA |
| mm4_intron_Zcchc14_chr8_121642447 |
CTGAAGGCCCCAGTGTAGCCCTGGGAGAGGACTGGAGATCCCAAGCCAGAGTCCTGCAGGGGGCCTGACAACGCTGAACG GCCCTGCACACCTCTGCAGCCTCCTAGACCAAAGCTGCCTTCCACCTCATTC |
| mm4_intron_Cdhr3_chr12_33046268 |
GGAGAAGTGTTGCTGAGGACATGTTTCAGAACGCATTTAATCTATTTAATCTATTCTTATCCCCAGAGAGGCCTGGGAGT CCCTTCCCCCCACAGCACTTTTCCAGGA |
| mm4_exon_Dnah2_chr11_69429477 |
AGAGACAGGTGGCAGTTTGCCAGGAACACCCAGTGCCCTGCGAGGAAGTGGAGCTGGTGTTAAATGTCTGTTATTTCTGG GATTTCTAGAAAAGCAGGAACTGAAATCATATAGTGGGGGCTGGGAGGTTCTTGGGAAA |
| mm4_intron_Bahcc1_chr11_120259739 |
GGTACCTGTTGCCCTCAGACACCATGAAACAGTGGTCTCTGGAGCCCTGTTGGGACCTCCAGGCCTCTCCCGGGCTTTTC CCAGAATACAACTCACCTTGTCCAGACGTCTGAGGTCT |
| mm4_intron_Lhx8_chr3_154328307 |
GTTCCCAGACCAGCAGGACCCCTGGANNNNNNNNNNNNNNNNNNNNNAGGGAGCTGGGGGTCCCCACTCGGGTCGCCGCC CCACTCACCAGTCCTTCTTCCCCA |
| mm4_intron_Brd4_chr17_32198321 |
CTTCATCCTCTCGGCTCCTGGGCAGAGCGGGGCCCCAGGTCAGCCTGGAGCCCAGTATGGCCCTGGGAGCCCCAGTGCCC TGCATGGACCCCAGCCCACCTCATGCGCTCCTGCCTCAGCCGCTCCTTCTCCTTCTCTGC |
| mm4_intron_Apba1_chr19_23878393 |
TGTTTCTGGAGAACAAACATGAAGGCCACAGACTGGACTGGGGTTCCCTAAGATGCATAACTCTTGGCACCATTTTTACT ATCTGGCCCTACCCCTG |
| mm4_intron_Opcml_chr9_28882554 |
GTGTGTCTGGCCTCTGGATGCTCCCTGACTCCCCTGCTGCACACTCAGTTGTCCTTATCACAAGCTTTCATCATCATCTT GAAACCCTGGGACCACCAGTTCCCTGGGAGGAGAGATTGCCTTGTGTATTTGCAGTCC |
| mm4_intergenic_Sox2ot|Gm20446_chr3_34212143 |
GCTGGAACTAGCTCCCCTTGTCTGTGTGTGCTTCCTGGCTTTATCCTGGGGTCCCCAGTCCGCTCCCGGGGAGGTGTAGC TTGCACCCAGTCT |
| mm4_intergenic_Ces5a|Gm26843_chr8_93703333 |
CAGAGCACACTCCTTCTGCACCTGATAGCATACCACCATGGGGCTCCCCAGTCCTCTCTGTGGAGTACCAAGAGATGTGA TCAGAAAAATAAACAGCACCTACAGCACACCCCACCCATGCTTTGTCACATGCC |
| mm4_intergenic_Gm23905|Zfp36l2_chr17_84013641 |
CTAACCTTGTCAGCCCTGCAAATCACCAGAACGGATTCCCTCGGAGAGCACTGGGGGCCACCTCGCTGAGCTCTGTGTTC AACACCTGCTTCCCCTTCCCTTCTG |
| mm4_exon_Tmem44_chr16_30550300 |
GGACTTGAGCTACCTGCAGGACGCCCCCTGTACGCCCCTTCCCCCTAGGCTTCCTAGAGGAGCCGAGGCGAGTGACGCCC AGTCGCCCCCGCGGGTGGCCCGCCACCCCTCGCGCGGGTCCCGTCCCCATTACAGCGTGT |
| mm4_exon_Gtf2i_chr5_134238169 |
AACCAACCAGGCAGACACACAGCCCAGCCTGACTCGACCCAGACCCAGAGCTGCGGCCCAGAGCCCCTGAGGCCACTGTG GGTCCCAGTTGGGGAGGGGCAAATCCTCCACCTGCTCAAGC |