← return to the list of all guides
Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313991_F | TCGTCGGCAGCGTCAACCGCCAGAGAAGATGGTG | 60.0 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313991_R | GTCTCGTGGGCTCGGCTGCCTGCTAGTCCATCGAC | 60.2 | 1.00 |
| mm3_exon_Slc17a4_chr13_23914930_F | TCGTCGGCAGCGTCACCGTCAGAACCAGAGCAAG | 59.9 | 0.44 |
| mm3_exon_Slc17a4_chr13_23914930_R | GTCTCGTGGGCTCGGCCTCTGGGTTAGACAGCTGC | 60.1 | 0.44 |
| mm4_intron_Dag1_chr9_108251346_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intron_Dag1_chr9_108251346_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intron_Syt7_chr19_10422838_F | TCGTCGGCAGCGTCTCCTGTCAGGCGCCAAAG | 59.9 | 0.37 |
| mm4_intron_Syt7_chr19_10422838_R | GTCTCGTGGGCTCGGCTTCTCAAGGCGTCCTCTCC | 59.8 | 0.37 |
| mm3_exon_Dgat1_chr15_76511727_F | TCGTCGGCAGCGTCCTACCACCCTGGACGGAAAC | 60.0 | 0.32 |
| mm3_exon_Dgat1_chr15_76511727_R | GTCTCGTGGGCTCGGCTCCAAGCGCTGAGAGCC | 60.5 | 0.32 |
| mm4_exon_Elf3/Gm26642_chr1_135255024_F | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_exon_Elf3/Gm26642_chr1_135255024_R | Primer3: not found at this Tm | N.d. | 0.31 |
| mm4_intron_Sohlh2_chr3_55207134_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Sohlh2_chr3_55207134_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Mir3099|Gm22649_chr7_6830751_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Mir3099|Gm22649_chr7_6830751_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Lamp1_chr8_13159556_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Lamp1_chr8_13159556_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_2010015L04Rik_chr4_155424270_F | TCGTCGGCAGCGTCCACTGTTCTGAGAGGGCCTC | 59.7 | 0.21 |
| mm4_intron_2010015L04Rik_chr4_155424270_R | GTCTCGTGGGCTCGGGTGGCCCACTCTTCCCTAAC | 60.0 | 0.21 |
| mm4_intergenic_Banp|Gm22_chr8_122080409_F | TCGTCGGCAGCGTCCTGATGTTGGAGGAGGAGGC | 59.8 | 0.18 |
| mm4_intergenic_Banp|Gm22_chr8_122080409_R | GTCTCGTGGGCTCGGGATCTCTCCCTGGGGATGGT | 60.1 | 0.18 |
| mm4_exon_Tada3_chr6_113370321_F | TCGTCGGCAGCGTCGACCTCGTCCTCTGAGTCCT | 60.0 | 0.18 |
| mm4_exon_Tada3_chr6_113370321_R | GTCTCGTGGGCTCGGTGTGCCACATACCAAGTCCC | 59.9 | 0.18 |
| mm4_intron_Dynll2_chr11_87986964_F | TCGTCGGCAGCGTCGAGAAAGCATCCACCTCCCC | 60.1 | 0.16 |
| mm4_intron_Dynll2_chr11_87986964_R | GTCTCGTGGGCTCGGCCTTCCTGCAGCGAGGTC | 60.1 | 0.16 |
| mm4_exon_Snx21/Acot8_chr2_164792287_F | TCGTCGGCAGCGTCCCCTTTCTTGAGGCCCATGT | 59.9 | 0.16 |
| mm4_exon_Snx21/Acot8_chr2_164792287_R | GTCTCGTGGGCTCGGAAGGCCTGCTTCCTGTAGTG | 59.6 | 0.16 |
| mm3_exon_Bnc2_chr4_84675214_F | TCGTCGGCAGCGTCGTGACTGATTTACACCCGCG | 59.2 | 0.16 |
| mm3_exon_Bnc2_chr4_84675214_R | GTCTCGTGGGCTCGGTTCTTTTCTCCCTCTGCCCG | 59.6 | 0.16 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36697467_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm25247|Tshz3_chr7_36697467_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Abhd3_chr18_10706261_F | TCGTCGGCAGCGTCGACTTAGCAGTGTCCCCACC | 60.0 | 0.14 |
| mm4_intron_Abhd3_chr18_10706261_R | GTCTCGTGGGCTCGGATTCTGTGCGTCAGTCTCCG | 60.1 | 0.14 |
| mm4_exon_Uso1_chr5_92137995_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_exon_Uso1_chr5_92137995_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm13518|mmu-mir-195b_chr2_56532310_F | TCGTCGGCAGCGTCACTGGATTTGACTAATGGGCA | 57.2 | 0.13 |
| mm4_intergenic_Gm13518|mmu-mir-195b_chr2_56532310_R | GTCTCGTGGGCTCGGAGCTGCTTTATCTGACTGGCT | 59.4 | 0.13 |
| mm4_intron_Hmcn2_chr2_31329578_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Hmcn2_chr2_31329578_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Acot7_chr4_152267929_F | TCGTCGGCAGCGTCCTGTCAGTCACTGGCCAAGA | 59.6 | 0.11 |
| mm4_intron_Acot7_chr4_152267929_R | GTCTCGTGGGCTCGGTCCTGGAGGTCCTATCACGG | 60.1 | 0.11 |
| mm4_exon_Oaf_chr9_43239631_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Oaf_chr9_43239631_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Ccnd1|Tpcn2_chr7_145233374_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Ccnd1|Tpcn2_chr7_145233374_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm24614|Stmn2_chr3_8462960_F | TCGTCGGCAGCGTCTGAACACCACTCCTGGAAGC | 59.8 | 0.10 |
| mm4_intergenic_Gm24614|Stmn2_chr3_8462960_R | GTCTCGTGGGCTCGGCGGAGCTGGACGAGGACAA | 61.6 | 0.10 |
| mm4_intergenic_Gm10398|Gm26660_chr14_25026816_F | TCGTCGGCAGCGTCTAGGTCCTCCCCTTGCTTGT | 60.1 | 0.09 |
| mm4_intergenic_Gm10398|Gm26660_chr14_25026816_R | GTCTCGTGGGCTCGGACAGTGGGGATGTGCAGTG | 59.9 | 0.09 |
| mm4_intergenic_Gm22983|1700057H15Rik_chr4_124396020_F | TCGTCGGCAGCGTCGGAAGTGGCAGGATTCCACA | 59.9 | 0.09 |
| mm4_intergenic_Gm22983|1700057H15Rik_chr4_124396020_R | GTCTCGTGGGCTCGGTCCTGCCACTCAAGTGTGTC | 59.8 | 0.09 |
| mm4_intron_Susd4_chr1_182862826_F | TCGTCGGCAGCGTCCCAAAGTGCACATCAGAGCG | 59.8 | 0.08 |
| mm4_intron_Susd4_chr1_182862826_R | GTCTCGTGGGCTCGGCACCACTGCTCAGACTCTGG | 60.0 | 0.08 |
| mm4_intergenic_Cldn10|n-R5s51_chr14_118804608_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Cldn10|n-R5s51_chr14_118804608_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm16134|Fancc_chr13_63464109_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm16134|Fancc_chr13_63464109_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_exon_Tm9sf3_chr19_41263872_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Tm9sf3_chr19_41263872_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Kifc3|Cngb1_chr8_95149653_F | TCGTCGGCAGCGTCATCTGGTGACATGTCTGCCG | 60.1 | 0.06 |
| mm4_intergenic_Kifc3|Cngb1_chr8_95149653_R | GTCTCGTGGGCTCGGGCGTCAAAACTTCCGTTGCT | 59.9 | 0.06 |
| mm4_exon_Tmem189/Gm20431_chr2_167661420_F | TCGTCGGCAGCGTCCCTACCTGGCGAGTAAAGCG | 60.5 | 0.06 |
| mm4_exon_Tmem189/Gm20431_chr2_167661420_R | GTCTCGTGGGCTCGGGGAGCTGGACGAGGACGA | 60.7 | 0.06 |
| mm3_exon_Irf2bp2_chr8_126593052_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_exon_Irf2bp2_chr8_126593052_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Stat5b_chr11_100839517_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Stat5b_chr11_100839517_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_D430019H16Rik|Bdkrb2_chr12_105515449_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_D430019H16Rik|Bdkrb2_chr12_105515449_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Btbd7|Cox8c_chr12_102878526_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Btbd7|Cox8c_chr12_102878526_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_2410076I21Rik|Hcn4_chr9_58802757_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_2410076I21Rik|Hcn4_chr9_58802757_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Hivep3_chr4_120045923_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Hivep3_chr4_120045923_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_1500009L16Rik_chr10_83731728_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_1500009L16Rik_chr10_83731728_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Wnt2_chr6_17991796_F | TCGTCGGCAGCGTCGCCGAAAGCAGGAAGACAGA | 60.3 | 0.02 |
| mm4_intron_Wnt2_chr6_17991796_R | GTCTCGTGGGCTCGGAGCATGATGTAGGCTCAGGG | 59.2 | 0.02 |
| mm4_intron_Ppl_chr16_5111511_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Ppl_chr16_5111511_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26430|Gm23895_chr18_78646146_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm26430|Gm23895_chr18_78646146_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm22112|Rhbdd1_chr1_82411597_F | TCGTCGGCAGCGTCTTTTCCACTGTGCAGGAACT | 57.5 | 0.01 |
| mm4_intergenic_Gm22112|Rhbdd1_chr1_82411597_R | GTCTCGTGGGCTCGGTGCAGTACATCATCAGCGCA | 60.1 | 0.01 |
| mm4_exon_Zfp783/Zfp956_chr6_47951936_F | TCGTCGGCAGCGTCCTGTCCCTACTGTGGCAAGG | 60.0 | 0.01 |
| mm4_exon_Zfp783/Zfp956_chr6_47951936_R | GTCTCGTGGGCTCGGGGCACTGATAGGAACGCTCA | 59.8 | 0.01 |
| mm4_intron_Nbea_chr3_55885970_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Nbea_chr3_55885970_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tenm3_chr8_48248702_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tenm3_chr8_48248702_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rsph10b_chr5_143962772_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Rsph10b_chr5_143962772_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gm16226_chr5_99714553_F | TCGTCGGCAGCGTCTAGCCTGGGTGTCCCTCC | 59.9 | 0.00 |
| mm4_intron_Gm16226_chr5_99714553_R | GTCTCGTGGGCTCGGACCTTCCAAAGCATGTCTCCT | 59.2 | 0.00 |
| mm4_intergenic_Sfxn3|Kazald1_chr19_45058641_F | TCGTCGGCAGCGTCCTCTTGATCCTGGACCGCTC | 59.8 | 0.00 |
| mm4_intergenic_Sfxn3|Kazald1_chr19_45058641_R | GTCTCGTGGGCTCGGCGCGTGATTCCTCGAAAACC | 59.9 | 0.00 |
| mm4_intergenic_Gm24051|Gm11988_chr11_8047066_F | TCGTCGGCAGCGTCGAATCACTGGAATGTGCAGGC | 59.8 | 0.00 |
| mm4_intergenic_Gm24051|Gm11988_chr11_8047066_R | GTCTCGTGGGCTCGGACACACCTTAGACAGAGGAGAC | 58.8 | 0.00 |
| mm4_intergenic_Gm22713|Prss12_chr3_123421693_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm22713|Prss12_chr3_123421693_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Adcy6|Cacnb3_chr15_98631851_F | TCGTCGGCAGCGTCGCTGGGCTGTCTCCTAAAGG | 60.1 | 0.00 |
| mm4_intergenic_Adcy6|Cacnb3_chr15_98631851_R | GTCTCGTGGGCTCGGGGTGTTCAGTCCGTTTGACC | 59.0 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313991 |
AACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGA GGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAG |
| mm3_exon_Slc17a4_chr13_23914930 |
ACCGTCAGAACCAGAGCAAGAACTGGAAAGCAAACCTGAGGAAGGCGGAGAGCCGGCTCTTGGCTGGGATCCTATCGGTT TCTCAGCTTTCTGCAGCTGTCTAACCCAGAGG |
| mm4_intron_Syt7_chr19_10422838 |
TCCTGTCAGGCGCCAAAGTGGCCACTGCGGCGGCGGGGCTGGCGGTGGAGCGGGAAGGCCGGCTCGGGGAGAAGCCGGCG CCGGTGCCACCACCCGGAGAGGACGCCTTGAGAAG |
| mm3_exon_Dgat1_chr15_76511727 |
CTACCACCCTGGACGGAAACCCGCGAGCCGGTCCTCCGACGCCGAGAGCTTCCCGCGCCTCCGCGGTCGCCCATGGCTTC GGCCCGCACCTCGTCGCGGCCCAGTCGTGCTCCGCCGGCTCTCAGCGCTTGGAG |
| mm4_intron_2010015L04Rik_chr4_155424270 |
CACTGTTCTGAGAGGGCCTCATCTCAGTGCAACAGTGGTCACAGGGCCCAATGCAGTGTGAGGGCCAGCTCGAGGGCTCA GAACTGGGTTAGGGAAGAGTGGGCCAC |
| mm4_intergenic_Banp|Gm22_chr8_122080409 |
CTGATGTTGGAGGAGGAGGCCTGGCCCGCTGCTGCTGGGAAGCTGGCGGGCAGGCTCGGGGTCCACAGTGGGTCAGAGCT TATCTTGCTGGAAGCCTGGGCCTTACAGCATGGCATCACCATCCCCAGGGAGAGATC |
| mm4_exon_Tada3_chr6_113370321 |
GACCTCGTCCTCTGAGTCCTCCGCGGGCCGGTCCTCAGACTCCAGGAGGCCCTGGGCAATCAGCTCCTCCTTGATGCGGC TCTCCAGGGACTTGGTATGTGGCACA |
| mm4_intron_Dynll2_chr11_87986964 |
GAGAAAGCATCCACCTCCCCGCCGCCCTCCGGATCCGCAGGCCGCAAGCCGGTCCTCCGCCCTCCCCCGCCAGGCCTGGA GAGGCGCCGGAGTGACCCGGCGACCTCGCTGCAGGAAGG |
| mm4_exon_Snx21/Acot8_chr2_164792287 |
CCCTTTCTTGAGGCCCATGTCCGGCTCTCCTGGCGCCTGGGCCTGGACAAACGGCAGTCGGAGGCCCAGCTCCAGGCACT ACAGGAAGCAGGCCTT |
| mm3_exon_Bnc2_chr4_84675214 |
GTGACTGATTTACACCCGCGCCCAGGGTCGCGCTCGCTCCCTCGAGCCGGCGCTCCGAGTTGTCCCGCTCCTCCCCGGGC AGAGGGAGAAAAGAA |
| mm4_intron_Abhd3_chr18_10706261 | GACTTAGCAGTGTCCCCACCCGAAGGCCTCGAGCGAGCCCTCCGATTCCATCTCCCCGGAGACTGACGCACAGAAT |
| mm4_intergenic_Gm13518|mmu-mir-195b_chr2_56532310 |
ACTGGATTTGACTAATGGGCAAGATATAAATTTACTGATGGTAGCAGAATTCAGAGGTCCGGCTCAAGGCCATAAAAATG AGATATAAAAAAGAATAGGTTATTAGTGGTCAACTAGCCAGTCAGATAAAGCAGCT |
| mm4_intron_Acot7_chr4_152267929 |
CTGTCAGTCACTGGCCAAGAAGGCCTGCCTGCCTCTGCTGTCTCCCCCAGCTGGCTCTCTGACTTCTGCTGCCGTGATAG GACCTCCAGGA |
| mm4_intergenic_Gm24614|Stmn2_chr3_8462960 |
TGAACACCACTCCTGGAAGCGCTTGCCTGGCGAGTAAAGCGCCGCCAGCTCACGGGCTCCCGTGTGCTGTGTGCCCCAGC GCCGACCCTCCGCCTTCTTGTCCTCGTCCAGCTCCG |
| mm4_intergenic_Gm10398|Gm26660_chr14_25026816 |
TAGGTCCTCCCCTTGCTTGTTATGTGAGCCTTCCAGTACATCACCTCAAGCTGGCGCTCCCACTTCTTTCCCTGAGTAAG AGAGCCAGGTCCCCACTGCACATCCCCACTGT |
| mm4_intergenic_Gm22983|1700057H15Rik_chr4_124396020 |
GGAAGTGGCAGGATTCCACAGGAGACCGGACACTCAAGATGGGCACCAGCCTGACCCGCCAGCCAGCTCTCTGACTTCCC CGGGACACACTTGAGTGGCAGGA |
| mm4_intron_Susd4_chr1_182862826 |
CCAAAGTGCACATCAGAGCGTCTGCTCTGGGGAGGGGTGGGAGGGCAGGCTCGTGGGAGACCTCACAATTGCAACGTGAA ATTTCTACCCAGGGATAGATGGCTCTTTATTTTCCCAGAGTCTGAGCAGTGGTG |
| mm4_intergenic_Kifc3|Cngb1_chr8_95149653 |
ATCTGGTGACATGTCTGCCGCAGCCACTACGGCATCACCTGGCCGAGGCCACCACGACCAGCTGCTCCCTCTAGCCGGCC CTCCCTCCTCTGGGGGTGGCCCTTGTTTGCAAACAGCAACGGAAGTTTTGACGC |
| mm4_exon_Tmem189/Gm20431_chr2_167661420 |
CCTACCTGGCGAGTAAAGCGCCGCCAGCTCACGGGCTCCCGCGTGCTGTGCGCCCCAGCGCCGGCCCTCCGCCGTCTCGT CCTCGTCCAGCTCC |
| mm4_intron_Wnt2_chr6_17991796 |
GCCGAAAGCAGGAAGACAGACAGAGGAGTTTCCTGAGGACAGGAAGGATGGCAGAGGGCCGGGTCGGGGTGGAGGGTGGG TCAGCCAGGATGGCCCTGAGCCTACATCATGCT |
| mm4_intergenic_Gm22112|Rhbdd1_chr1_82411597 |
TTTTCCACTGTGCAGGAACTGGTAAAGATCAGAGATGCTAATCTTTCTAGAGAGAAATAGGCAAGAAGACGGAGGGCAGG CAGGAGGAAGGCATAGGTGTGCGCTGATGATGTACTGCA |
| mm4_exon_Zfp783/Zfp956_chr6_47951936 |
CTGTCCCTACTGTGGCAAGGCCTCCCGCCGGCCCTCAGACCTCTTTTGTCACCAGTGTATCCATACTGGTGAGCGTTCCT ATCAGTGCC |
| mm4_intron_Gm16226_chr5_99714553 |
TAGCCTGGGTGTCCCTCCCTCACCCCCCGCCCCCGCCCTCCGACTGCCACATACACATACATCCAGGAACCACTTTAAAG AGAAATATGTTTTAAAGAGGAGACATGCTTTGGAAGGT |
| mm4_intergenic_Sfxn3|Kazald1_chr19_45058641 |
CTCTTGATCCTGGACCGCTCAAGCCGCCAAATCCCCCTCCTCCTTCTCCAGTTTCTAAACCCTAGAGCCACCGTGCGCCC GCCCTCTGACTTCCTGCCCGGAAATCCCGAGGTTTTCGAGGAATCACGCG |
| mm4_intergenic_Gm24051|Gm11988_chr11_8047066 |
GAATCACTGGAATGTGCAGGCCCACGATGTGGCCCTCCCACTTCCAGGTTTATGTGCCCTCAATTTATAATCTGTTTCTA TGTCTCAGACACAAGAAACACATTGCTTAGTCTCCTCTGTCTAAGGTGTGT |
| mm4_intergenic_Adcy6|Cacnb3_chr15_98631851 |
GCTGGGCTGTCTCCTAAAGGATTCTGCAGAAGCCTGGTCGGGACCAGGACAGGGTGGATGGTCGTGGCTTCCCCAGAAGT CAGAGGGGCGTCTTGAGGCTTGGTCAAACGGACTGAACACC |