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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313958_F | TCGTCGGCAGCGTCAATTTGTCCGAAGCCGTGAG | 58.8 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313958_R | GTCTCGTGGGCTCGGCTCCTCCTCCTGGCCTCC | 60.4 | 1.00 |
| mm3_intron_Elf2_chr3_51317899_F | TCGTCGGCAGCGTCGCTTCATGGAGCCTCAACCT | 60.0 | 0.57 |
| mm3_intron_Elf2_chr3_51317899_R | GTCTCGTGGGCTCGGGCTCTTGTGAGGGATGCAGT | 60.0 | 0.57 |
| mm4_intergenic_Gm11813|1700123O12Rik_chr4_10693499_F | Primer3: not found at this Tm | N.d. | 0.55 |
| mm4_intergenic_Gm11813|1700123O12Rik_chr4_10693499_R | Primer3: not found at this Tm | N.d. | 0.55 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202304_F | TCGTCGGCAGCGTCGAGGTGGTGCTCCAGAGAAG | 59.7 | 0.35 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202304_R | GTCTCGTGGGCTCGGCTGGAGCACCACCTTCTCTG | 60.0 | 0.35 |
| mm4_intergenic_Gm10184|Nrxn1_chr17_90014787_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Gm10184|Nrxn1_chr17_90014787_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Pcdh8|Gm6999_chr14_79793742_F | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Pcdh8|Gm6999_chr14_79793742_R | Primer3: not found at this Tm | N.d. | 0.29 |
| mm4_intergenic_Speer4f|Sema3c_chr5_17510232_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Speer4f|Sema3c_chr5_17510232_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm19932|Kcnip4_chr5_49440434_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm19932|Kcnip4_chr5_49440434_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm3_intergenic_St6galnac3|Gm10042_chr3_153744059_F | TCGTCGGCAGCGTCAAGCATCAGGGATCCATGGC | 60.1 | 0.25 |
| mm3_intergenic_St6galnac3|Gm10042_chr3_153744059_R | GTCTCGTGGGCTCGGACCATGTGCTTGGCAGTAGG | 60.3 | 0.25 |
| mm3_intron_Csn1s2a_chr5_87783281_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_intron_Csn1s2a_chr5_87783281_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Slc24a3_chr2_145628588_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intron_Slc24a3_chr2_145628588_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94271847_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_94271847_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm379|Brwd3_chrX_108677083_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Gm379|Brwd3_chrX_108677083_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_exon_Dnaaf3_chr7_4530772_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_exon_Dnaaf3_chr7_4530772_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Atp11c|Gm14661/Gm14651_chrX_60607604_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Atp11c|Gm14661/Gm14651_chrX_60607604_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Mitf_chr6_98008323_F | TCGTCGGCAGCGTCGCAGAAGAGCACAGGTCCAT | 60.0 | 0.12 |
| mm4_intron_Mitf_chr6_98008323_R | GTCTCGTGGGCTCGGTACAAAGATCTGGTGGCCCC | 59.3 | 0.12 |
| mm4_intron_1700085B03Rik_chr5_127264596_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_1700085B03Rik_chr5_127264596_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202321_F | TCGTCGGCAGCGTCGAGGTGGTGCTCCAGAGAAG | 59.7 | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202321_R | GTCTCGTGGGCTCGGCTGGAGCACCACCTTCTCTG | 60.0 | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202254_F | TCGTCGGCAGCGTCCTCCAGGGAAGATGGTGCTC | 59.8 | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202254_R | GTCTCGTGGGCTCGGCTGGAGCACCACCTTCTCTG | 60.0 | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202136_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202136_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202102_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202102_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202068_F | TCGTCGGCAGCGTCCGAGAGGGGTGGCAAGTATG | 60.1 | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202068_R | GTCTCGTGGGCTCGGCTGGAGCACCACCTTCTCTG | 60.0 | 0.11 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202170_F | TCGTCGGCAGCGTCCAGAGAAGGTGGTGCTCCAG | 60.0 | 0.10 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202170_R | GTCTCGTGGGCTCGGCACCTTCTCTGGAGCACCAC | 60.3 | 0.10 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202153_F | TCGTCGGCAGCGTCGTGGTGCTCCAGAGAAGGTG | 60.3 | 0.10 |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202153_R | GTCTCGTGGGCTCGGCTGGAGCACCACCTTCTCTG | 60.0 | 0.10 |
| mm4_exon_Slc28a1_chr7_81169986_F | TCGTCGGCAGCGTCTAGAGACCTTCTTCCGCCCA | 59.9 | 0.10 |
| mm4_exon_Slc28a1_chr7_81169986_R | GTCTCGTGGGCTCGGCCACCAAGCTCGCATCAAAC | 60.1 | 0.10 |
| mm4_intergenic_Rngtt|Gm12398_chr4_33570053_F | TCGTCGGCAGCGTCCCATTCTTCCTGGGTCCACC | 60.0 | 0.10 |
| mm4_intergenic_Rngtt|Gm12398_chr4_33570053_R | GTCTCGTGGGCTCGGCTTGCCTCTCCTCTGCTGAC | 60.1 | 0.10 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202371_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202371_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202287_F | TCGTCGGCAGCGTCCAGAGAAGGTGGTGCTCCAG | 60.0 | 0.10 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202287_R | GTCTCGTGGGCTCGGCACCTTCTCTGGAGCACCAC | 60.3 | 0.10 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202220_F | TCGTCGGCAGCGTCCTCCAGGGAAGATGGTGCTC | 59.8 | 0.10 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202220_R | GTCTCGTGGGCTCGGCTGGAGCACCACCTTCTCTG | 60.0 | 0.10 |
| mm4_intergenic_Zfp703|Erlin2_chr8_27020455_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Zfp703|Erlin2_chr8_27020455_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm3_intron_Ptchd2_chr4_148286076_F | TCGTCGGCAGCGTCGGGTGGATGGAAACTGCAGA | 59.9 | 0.09 |
| mm3_intron_Ptchd2_chr4_148286076_R | GTCTCGTGGGCTCGGTTGCTGGAGTCTGTGCTCTG | 59.9 | 0.09 |
| mm4_intergenic_Atp13a3|Tmem44_chr16_30478883_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Atp13a3|Tmem44_chr16_30478883_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Tbc1d7|Gfod1_chr13_43195395_F | TCGTCGGCAGCGTCTGATCCAGAGCACCACATCG | 59.8 | 0.08 |
| mm4_intergenic_Tbc1d7|Gfod1_chr13_43195395_R | GTCTCGTGGGCTCGGATCACTACGGAGCTGGAGGT | 60.0 | 0.08 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202237_F | TCGTCGGCAGCGTCCTCCAGGGAAGATGGTGCTC | 59.8 | 0.07 |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202237_R | GTCTCGTGGGCTCGGCTGGAGCACCACCTTCTCTG | 60.0 | 0.07 |
| mm4_exon_Foxb1/B230323A14Rik_chr9_69760136_F | TCGTCGGCAGCGTCGCGTGTTCTCCCGGTAGTAG | 59.9 | 0.07 |
| mm4_exon_Foxb1/B230323A14Rik_chr9_69760136_R | GTCTCGTGGGCTCGGTCATACATCTCGCTGACCGC | 59.9 | 0.07 |
| mm4_intron_Tmem132c_chr5_127364595_F | TCGTCGGCAGCGTCAAATGCAGCCAGTGGACAGA | 59.8 | 0.07 |
| mm4_intron_Tmem132c_chr5_127364595_R | GTCTCGTGGGCTCGGGATCAGCAGAGTGGGCAGAG | 60.1 | 0.07 |
| mm4_intergenic_4933413C19Rik|Glis3_chr19_28622827_F | TCGTCGGCAGCGTCACATGGCTCCCACCTCTCTC | 60.9 | 0.07 |
| mm4_intergenic_4933413C19Rik|Glis3_chr19_28622827_R | GTCTCGTGGGCTCGGGGTGCTCGAGAAGTTCCTGT | 59.6 | 0.07 |
| mm4_intergenic_Opcml|Gm15606_chr9_28137594_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Opcml|Gm15606_chr9_28137594_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm3_intron_Arhgef15_chr11_68951306_F | TCGTCGGCAGCGTCCCAGAGGGGATGCTCATCAG | 59.6 | 0.06 |
| mm3_intron_Arhgef15_chr11_68951306_R | GTCTCGTGGGCTCGGGAGTCCAGAGCGTCAGTGAG | 59.8 | 0.06 |
| mm4_intron_Col27a1_chr4_63279562_F | TCGTCGGCAGCGTCCTGACTGCATGGCCTCCTTT | 60.3 | 0.06 |
| mm4_intron_Col27a1_chr4_63279562_R | GTCTCGTGGGCTCGGATCCGCAAGAGCTTGGTCTC | 60.1 | 0.06 |
| mm4_intergenic_Tex29|A230072I06Rik_chr8_12230677_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Tex29|A230072I06Rik_chr8_12230677_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Amer2|Spata13_chr14_60419109_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Amer2|Spata13_chr14_60419109_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_1700040L02Rik|Tmem26_chr10_68697732_F | TCGTCGGCAGCGTCCGTTGTATGGGTGGTGGGAA | 59.9 | 0.06 |
| mm4_intergenic_1700040L02Rik|Tmem26_chr10_68697732_R | GTCTCGTGGGCTCGGGATGGGGAGGAAAGGCAGAC | 60.1 | 0.06 |
| mm4_intergenic_Redrum|Zfp608_chr18_54562211_F | TCGTCGGCAGCGTCTGGTGCAGGGTACATGTCTG | 59.6 | 0.06 |
| mm4_intergenic_Redrum|Zfp608_chr18_54562211_R | GTCTCGTGGGCTCGGAAAACCTGGGCATCTGGGC | 60.6 | 0.06 |
| mm4_exon_Cdh1_chr8_106649189_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Cdh1_chr8_106649189_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Fam170a|Hdhd1a_chr18_50350852_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intergenic_Fam170a|Hdhd1a_chr18_50350852_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Trpv1_chr11_73260556_F | TCGTCGGCAGCGTCCTGATGCTGGGTCTTTGGGT | 59.9 | 0.05 |
| mm4_exon_Trpv1_chr11_73260556_R | GTCTCGTGGGCTCGGCAGAGTACAGCCAGCCAACA | 59.9 | 0.05 |
| mm4_intron_Tll2_chr19_41162632_F | TCGTCGGCAGCGTCCCTGGCTACATCTTCCTGGC | 60.1 | 0.04 |
| mm4_intron_Tll2_chr19_41162632_R | GTCTCGTGGGCTCGGGGGCTGAGGAAGTTCAGAGG | 59.7 | 0.04 |
| mm3_exon_D130017N08Rik_chr5_143764465_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_exon_D130017N08Rik_chr5_143764465_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Mdfic|Tfec_chr6_15996759_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Mdfic|Tfec_chr6_15996759_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Svep1_chr4_58095396_F | TCGTCGGCAGCGTCTGCCCCAGCTTGAGAAGAAG | 59.9 | 0.04 |
| mm4_intron_Svep1_chr4_58095396_R | GTCTCGTGGGCTCGGACCCCTGGCTAGGCTGTATA | 59.4 | 0.04 |
| mm4_intergenic_Ly6i|Ly6a_chr15_74990213_F | TCGTCGGCAGCGTCCTCCGCAAGTTACCTGACAGT | 59.9 | 0.04 |
| mm4_intergenic_Ly6i|Ly6a_chr15_74990213_R | GTCTCGTGGGCTCGGTAGTGCTTGTGACTGGCCAG | 59.9 | 0.04 |
| mm4_intergenic_Ly6f|9030619P08Rik_chr15_75355049_F | TCGTCGGCAGCGTCCCCCAAGTTACCTGACAGTGT | 59.5 | 0.04 |
| mm4_intergenic_Ly6f|9030619P08Rik_chr15_75355049_R | GTCTCGTGGGCTCGGGCACCAAGCACCAACACATT | 59.8 | 0.04 |
| mm4_intergenic_Ly6c2|Ly6g_chr15_75116165_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Ly6c2|Ly6g_chr15_75116165_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm20654|Ly6h_chr15_75467369_F | TCGTCGGCAGCGTCTCTGGCACCCAACACTGATC | 59.9 | 0.04 |
| mm4_intergenic_Gm20654|Ly6h_chr15_75467369_R | GTCTCGTGGGCTCGGGCACCAAGCACCAACACATT | 59.8 | 0.04 |
| mm4_intergenic_9030619P08Rik|Gm20654_chr15_75442488_F | TCGTCGGCAGCGTCATCAGTCTGAGGTTCCGCAG | 59.4 | 0.04 |
| mm4_intergenic_9030619P08Rik|Gm20654_chr15_75442488_R | GTCTCGTGGGCTCGGTAGTGCTTGTGACTGGCCAG | 59.9 | 0.04 |
| mm3_intergenic_Gm6189|Dock10_chr1_80385192_F | TCGTCGGCAGCGTCTTGTAGTGCAGACGGCCTTT | 59.8 | 0.03 |
| mm3_intergenic_Gm6189|Dock10_chr1_80385192_R | GTCTCGTGGGCTCGGTCTCTCGTGTCTTAGTCCTCCT | 59.4 | 0.03 |
| mm4_intron_Zmiz1_chr14_25642572_F | TCGTCGGCAGCGTCAAGTCAGCACAGACCAGTGG | 59.8 | 0.03 |
| mm4_intron_Zmiz1_chr14_25642572_R | GTCTCGTGGGCTCGGAAACACTGTACCTGTGCCCC | 60.1 | 0.03 |
| mm2_intergenic_D5Ertd579e|Bloc1s4_chr5_36731962_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm2_intergenic_D5Ertd579e|Bloc1s4_chr5_36731962_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm22764|Gm4822_chr14_112008115_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm22764|Gm4822_chr14_112008115_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm14062|Btbd3_chr2_138033554_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm14062|Btbd3_chr2_138033554_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_G630016G05Rik|Gm13991_chr2_116278094_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm3_intergenic_G630016G05Rik|Gm13991_chr2_116278094_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gm14268_chr2_166386931_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Gm14268_chr2_166386931_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Nav1|Nav1/Gm4793_chr1_135538874_F | TCGTCGGCAGCGTCTGGAAGGGCCATGCTGTATG | 60.1 | 0.03 |
| mm4_intergenic_Nav1|Nav1/Gm4793_chr1_135538874_R | GTCTCGTGGGCTCGGGAGAAGCTAGGGGACAGGGA | 60.0 | 0.03 |
| mm3_intergenic_Gldn|Dmxl2_chr9_54353437_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gldn|Dmxl2_chr9_54353437_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Btla|Cd200_chr16_45381208_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Btla|Cd200_chr16_45381208_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Angpt4_chr2_151934711_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Angpt4_chr2_151934711_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Nptx2|Gm6272_chr5_144594254_F | TCGTCGGCAGCGTCACCAGTGAGCCATCAACCAA | 59.5 | 0.02 |
| mm4_intergenic_Nptx2|Gm6272_chr5_144594254_R | GTCTCGTGGGCTCGGGAAATGCCATGCCCAGGTTC | 59.8 | 0.02 |
| mm4_intergenic_Hira|Gm10088_chr16_19022043_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Hira|Gm10088_chr16_19022043_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Hivep3_chr4_119987039_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Hivep3_chr4_119987039_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52056468_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52056468_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Zkscan5_chr5_145207599_F | TCGTCGGCAGCGTCTGCCACAAGTCCCGAAGTAC | 59.9 | 0.02 |
| mm4_exon_Zkscan5_chr5_145207599_R | GTCTCGTGGGCTCGGGGTGACTGAAGGACTCCTGC | 60.0 | 0.02 |
| mm4_intergenic_Vrk1|Gm24986_chr12_106136789_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Vrk1|Gm24986_chr12_106136789_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Gm25809_chr15_34288843_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Gm25809_chr15_34288843_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23117|Gm5083_chr13_44077575_F | TCGTCGGCAGCGTCGAACAGTCGATGGAGACCCC | 59.8 | 0.01 |
| mm4_intergenic_Gm23117|Gm5083_chr13_44077575_R | GTCTCGTGGGCTCGGTCCTCTTCATTTTAACTGCTGGGA | 59.9 | 0.01 |
| mm4_intergenic_Bod1l|mmu-mir-6414_chr5_41846952_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Bod1l|mmu-mir-6414_chr5_41846952_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm25011|Gm23680_chr10_12154646_F | TCGTCGGCAGCGTCCTGGTTGCTTTTGCCCACTC | 59.9 | 0.01 |
| mm4_intergenic_Gm25011|Gm23680_chr10_12154646_R | GTCTCGTGGGCTCGGACATCTCAGCTGCCATCAGT | 59.0 | 0.01 |
| mm4_intergenic_Rgs17|Gm10945_chr10_6001444_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Rgs17|Gm10945_chr10_6001444_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm3_intergenic_mmu-mir-6393|Zdhhc25_chr15_88202803_F | TCGTCGGCAGCGTCAGTGAAAGCAACTTCACCCA | 57.5 | 0.01 |
| mm3_intergenic_mmu-mir-6393|Zdhhc25_chr15_88202803_R | GTCTCGTGGGCTCGGTGCACTGGGACCTCACTCAT | 60.8 | 0.01 |
| mm4_intergenic_Defb28|Defb29_chr2_152534606_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Defb28|Defb29_chr2_152534606_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13061|Zfp683_chr4_134053023_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm13061|Zfp683_chr4_134053023_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Snord88c_chr7_44249887_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Snord88c_chr7_44249887_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm7190|Gm14643_chrX_40064901_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm7190|Gm14643_chrX_40064901_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Chchd6_chr6_89439875_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Chchd6_chr6_89439875_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ccser1_chr6_62231285_F | TCGTCGGCAGCGTCTCATCTTGCTCCCTACAAGATTCA | 59.5 | 0.00 |
| mm4_intron_Ccser1_chr6_62231285_R | GTCTCGTGGGCTCGGCCGAAGGCTGTTGTGTATGAG | 58.9 | 0.00 |
| mm4_intergenic_Gm24751|Angpt1_chr15_42331999_F | TCGTCGGCAGCGTCGGCACTTTTGAATGCAGAGGA | 59.1 | 0.00 |
| mm4_intergenic_Gm24751|Angpt1_chr15_42331999_R | GTCTCGTGGGCTCGGGCTGTACAAACTTTCACCACCA | 59.3 | 0.00 |
| mm4_intron_Pkd1l2_chr8_117050210_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pkd1l2_chr8_117050210_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12753|Gm12751_chr4_34157979_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12753|Gm12751_chr4_34157979_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Plcb3_chr19_6968386_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Plcb3_chr19_6968386_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zdhhc15|Gm14822_chrX_104746452_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Zdhhc15|Gm14822_chrX_104746452_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Abcc9|Cmas_chr6_142723917_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Abcc9|Cmas_chr6_142723917_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tmprss15_chr16_78968397_F | TCGTCGGCAGCGTCTGGGAGCATTGTAGACTATAAGAGA | 58.6 | 0.00 |
| mm4_intron_Tmprss15_chr16_78968397_R | GTCTCGTGGGCTCGGAGCACAGCCCTATTTTGAACA | 58.1 | 0.00 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116359852_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Maf/Gm15655|Dynlrb2_chr8_116359852_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rpsa-ps9|Gbgt1_chr2_28494995_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rpsa-ps9|Gbgt1_chr2_28494995_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Spag1_chr15_36187337_F | TCGTCGGCAGCGTCTGCAAGCACTTAGAGACCCC | 59.6 | 0.00 |
| mm4_intron_Spag1_chr15_36187337_R | GTCTCGTGGGCTCGGCTCCAACAGACCCCGTCTTC | 60.0 | 0.00 |
| mm4_intron_Rcan2_chr17_43909877_F | TCGTCGGCAGCGTCGAACATTCTGCCAGCAAGCC | 60.1 | 0.00 |
| mm4_intron_Rcan2_chr17_43909877_R | GTCTCGTGGGCTCGGAGCAGCCTTTCCCTCCAAAT | 59.5 | 0.00 |
| mm4_intron_Mad1l1_chr5_140165615_F | TCGTCGGCAGCGTCACGATCCAAGTGCACAGTGT | 59.8 | 0.00 |
| mm4_intron_Mad1l1_chr5_140165615_R | GTCTCGTGGGCTCGGAAGCCTGACTGATGTGGGTG | 59.9 | 0.00 |
| mm4_intron_Gpr123_chr7_139872914_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Gpr123_chr7_139872914_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dnah9_chr11_66142008_F | TCGTCGGCAGCGTCGTGGAGCAGAGCATGAGGAA | 59.7 | 0.00 |
| mm4_intron_Dnah9_chr11_66142008_R | GTCTCGTGGGCTCGGAGTGTGCGATCATGTGGTGT | 59.9 | 0.00 |
| mm4_intron_Dennd3_chr15_73527245_F | TCGTCGGCAGCGTCGAGCTGACTTTCCTGGGGAC | 60.0 | 0.00 |
| mm4_intron_Dennd3_chr15_73527245_R | GTCTCGTGGGCTCGGTGGTTGAGTGGAGATCGTGC | 60.0 | 0.00 |
| mm4_intron_Calb2_chr8_110150725_F | TCGTCGGCAGCGTCATGTCAGGGACAGAACTGCC | 59.6 | 0.00 |
| mm4_intron_Calb2_chr8_110150725_R | GTCTCGTGGGCTCGGCCAGGTGTGATGGGCAGAAA | 60.2 | 0.00 |
| mm4_intergenic_Tmem178b|Agk_chr6_40284004_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tmem178b|Agk_chr6_40284004_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mul1|Camk2n1_chr4_138449672_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mul1|Camk2n1_chr4_138449672_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Eln|Wbscr28_chr5_134847093_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Eln|Wbscr28_chr5_134847093_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cntnap2|Cul1_chr6_47364241_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cntnap2|Cul1_chr6_47364241_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intergenic_Slc39a11|Gm26052_chr11_113330467_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm2_intergenic_Slc39a11|Gm26052_chr11_113330467_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313958 |
AATTTGTCCGAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGC CGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAG |
| mm3_intron_Elf2_chr3_51317899 |
GCTTCATGGAGCCTCAACCTCTGGGTGATCCAGCCAGAGAAGACGGAGCCCCACGATGCATGCAGCTCTTTCTTTAGCGC GGGGCTTCTCTGCGTTTTGGACACTGCATCCCTCACAAGAGC |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202304 |
GAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAGAGAAGATGGTGCTCCAGAGAAGGTGGTGCTCCAGGGAGGTGGTGCTCC AGGGAAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAG |
| mm3_intergenic_St6galnac3|Gm10042_chr3_153744059 |
AAGCATCAGGGATCCATGGCCACATCTGACAATAAAACATTGGAGATCAGAGATGGGGAAGCCTGAGAAGATGCTGCTCC ACGAGACCCCAGCACCAGCAGGAGGCCTACTGCCAAGCACATGGT |
| mm4_intron_Mitf_chr6_98008323 |
GCAGAAGAGCACAGGTCCATTGCCAGAGAAGATGCTGTCACACTGGAAGGGGAGTACAGGGAGATATGGGCAGGGCAGGG CGGGGCCACCAGATCTTTGTA |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202321 |
GAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAGAGAAGATGGTGCTCCAGAGAAGGTGGTGCTCCAGGGAGGTGGTGCTCC AGGGAAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAG |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202254 |
CTCCAGGGAAGATGGTGCTCCAGGGAGGTGGTGCTCCAGGGAAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAGAGAATAT GGTGCTCCAGAGAAGGTGGTGCTCCAGGGAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAG |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202068 |
CGAGAGGGGTGGCAAGTATGTGGGTCTAGTGGTATGGCTAGAAGATGGTGCTCCAGAGAAGGTGGTGCTCCAGGGAAGGT GGTGCTCCAGAGAAGGTGGTGCTCCAG |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202170 |
CAGAGAAGGTGGTGCTCCAGGGAAGATGGTGCTCCAGGGAAGATGGTGCTCCAGGGAGGTGGTGCTCCAGGGAAGGTGGT GCTCCAGAGAAGGTG |
| mm3_intergenic_Efnb1|Gm14812_chrX_99202153 |
GTGGTGCTCCAGAGAAGGTGGTGCTCCAGGGAAGATGGTGCTCCAGGGAAGATGGTGCTCCAGGGAGGTGGTGCTCCAGG GAAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAG |
| mm4_exon_Slc28a1_chr7_81169986 |
TAGAGACCTTCTTCCGCCCAGTCTCCAAGAGTGCTTTCCACCCCAGTGTCCTGTGGGGCACCATCTCATTAGGTGTGATG TCAGAGTGGCCCAGGCTCCACTCTGACTCGGTAAAGTTTGATGCGAGCTTGGTGG |
| mm4_intergenic_Rngtt|Gm12398_chr4_33570053 |
CCATTCTTCCTGGGTCCACCCTGGGATGTTTGTTGTGAAACCTCATGAACCAAGATAGATGACAGATGTGTTAGAAAAGA GCCCAGAGAAGAGAGTGCTCCACAGAGAGTGCTCTGTGTCAGCAGAGGAGAGGCAAG |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202287 |
CAGAGAAGGTGGTGCTCCAGGGAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAGAGAAGATGGTGCTCCAGAGAAGGTGGT GCTCCAGGGAGGTGGTGCTCCAGGGAAGGTGGTGCTCCAGAGAAGGTG |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202220 |
CTCCAGGGAAGATGGTGCTCCAGGGAGGTGGTGCTCCAGGGAAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAGAGAATAT GGTGCTCCAGAGAAGGTGGTGCTCCAG |
| mm3_intron_Ptchd2_chr4_148286076 |
GGGTGGATGGAAACTGCAGATGAGGAAGCAAACCAGAGTCGATGGTGCCCCAAGGGCCAGTGGTGCCAACATGGGTGTCA GAGCACAGACTCCAGCAA |
| mm4_intergenic_Tbc1d7|Gfod1_chr13_43195395 |
TGATCCAGAGCACCACATCGGCACCAGTGGGGCATCATCCACTCAGGAGCCTGGAACTGCAGATCCCGAGGGAGGGCAAG CCACCTCCAGCTCCGTAGTGAT |
| mm4_intergenic_Efnb1|Gm14812_chrX_99202237 |
CTCCAGGGAAGATGGTGCTCCAGGGAGGTGGTGCTCCAGGGAAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAGAGAATAT GGTGCTCCAGAGAAGGTGGTGCTCCAGGGAGGTGGTGCTCCAGAGAAGGTGGTGCTCCAG |
| mm4_exon_Foxb1/B230323A14Rik_chr9_69760136 |
GCGTGTTCTCCCGGTAGTAGGGGAAGCGGTCCATGATGAACTTGTAGATCTCGCTGAGCGGCAGCATCTTCTCCGGAGAG CTCTGGATGGCCATGGCGGTCAGCGAGATGTATGA |
| mm4_intron_Tmem132c_chr5_127364595 |
AAATGCAGCCAGTGGACAGATGAGATGAGACCTTTGCCTGGGTGGGGCACTCTCTTCTCAGGTCCTGGATTTGGAGGAAG ATCATTGATGGGCCTTGCATGCTCTGCCCACTCTGCTGATC |
| mm4_intergenic_4933413C19Rik|Glis3_chr19_28622827 |
ACATGGCTCCCACCTCTCTCCAGAGAGACCTTTGGGAGCCATCTTCTCTGGCTCTTGGCCACCTGTTTATTCTAAGGTAC TGATGAAGAATATATATTGAATCAGTCTTGAACACATCACAGGAACTTCTCGAGCACC |
| mm3_intron_Arhgef15_chr11_68951306 |
CCAGAGGGGATGCTCATCAGCCCAAGGCCCAGAGTCCTGGGAAGATGGGGCCCCACTGACCGCTCACTGACGCTCTGGAC TC |
| mm4_intron_Col27a1_chr4_63279562 |
CTGACTGCATGGCCTCCTTTATTTAACATCAGAACTGAATTCAGCATCCCGGCTGCTCTGTTCCCAGAGGAGAAGGTGAC CCAAGGCAGGGATGGTTTCCTAGCTTAAGAGACCAAGCTCTTGCGGAT |
| mm4_intergenic_1700040L02Rik|Tmem26_chr10_68697732 |
CGTTGTATGGGTGGTGGGAATTGCTATACAGTTATTGATGTTGTTCTTCATAGACACACATCCCCGCTTTGGTCCACGTG GGGAAAGTCCTTGTGTCACCTTCTTCTCTGGCTCGTCTTTGTCTGCCTTTCCTCCCCATC |
| mm4_intergenic_Redrum|Zfp608_chr18_54562211 |
TGGTGCAGGGTACATGTCTGCAACGAGGAAGGAAAGATGTAGGTGAGATTTGGGTAGCACCATAGACTGTAAAATGTTCC TTAGGCCTCCATCTTCTCCAGAGAGAGCCCAGATGCCCAGGTTTT |
| mm4_exon_Trpv1_chr11_73260556 |
CTGATGCTGGGTCTTTGGGTGGCCCATTGCTCCCGTGGGGAAGGATCTACTCAGGGAGCAATAGAGCCGGGTTGATGTTG GCTGGCTGTACTCTG |
| mm4_intron_Tll2_chr19_41162632 |
CCTGGCTACATCTTCCTGGCTTCTCACGTCTCTCGTGGGACACCATTTCCTCTGGGACAGTGCAGGCTGGCACCCCTGCT CTTTCCAGCTGTAAGCCCAGCACCTCTGAACTTCCTCAGCCC |
| mm4_intron_Svep1_chr4_58095396 |
TGCCCCAGCTTGAGAAGAAGATCCCAGTAGGTCTCTGGGACCTGCAACCTGCTCATTAGGCCAGTGCTATGGGTGGACCT GAGAAGAAAGGGCCCCATGGAACACTATACAGCCTAGCCAGGGGT |
| mm4_intergenic_Ly6i|Ly6a_chr15_74990213 |
CTCCGCAAGTTACCTGACAGTGTGTATCAGTCTGAGGTTCAGCAGCCATGGGGAAGCATCTTCGCAGGAGCCTTTTATGT GAATAATGTGTTGGTGCTTGGTGCTGGGTCCTGGCCAGTCACAAGCACTA |
| mm4_intergenic_Ly6f|9030619P08Rik_chr15_75355049 |
CCCCAAGTTACCTGACAGTGTGTATCAGTCTGAGGTTCAGCAGCCATGGGGAAGCATCTTCGCAGGAGCCTTTTATGTGA ATAATGTGTTGGTGCTTGGTGC |
| mm4_intergenic_Gm20654|Ly6h_chr15_75467369 |
TCTGGCACCCAACACTGATCCTTGAACCCTCAACAGCAGTGTGTATCAGTCTGAGGTTCAGCAGCCATGGGGAAGCATCT TCGCAGGAGCCTTTTATGTGAATAATGTGTTGGTGCTTGGTGC |
| mm4_intergenic_9030619P08Rik|Gm20654_chr15_75442488 |
ATCAGTCTGAGGTTCCGCAGCCATGGGGAAGCATCTTCGCAGGAGCCTTTTATGTGAATAATGTGTTGGTGCTTGGTGCT GGGTCCTGGCCAGTCACAAGCACTA |
| mm3_intergenic_Gm6189|Dock10_chr1_80385192 |
TTGTAGTGCAGACGGCCTTTGCTGGAGTTGCGGAGCGCCTTCAGGGTTTCCCAGCTGTGACCATCGGACGGCCTTGACCT GGGGAGCACCATCTTCTCTGAAGGAGGACTAAGACACGAGAGA |
| mm4_intron_Zmiz1_chr14_25642572 |
AAGTCAGCACAGACCAGTGGAGGCAGACCCAGGGAAGAGGGTGCCACACGAGATTTTCTGCTGCTCTGGGGCACAGGTAC AGTGTTT |
| mm4_intergenic_Nav1|Nav1/Gm4793_chr1_135538874 |
TGGAAGGGCCATGCTGTATGGCTCTTCTCAGAGTCTCTGGGGCACCTTCTTCTTGGGCCAGCTCCAATCCTGAGCCCAGC CATCTCTGTCTTCCTCCCTTGAGGTCCTGTCCCTGTCCCCTAGCTTCTC |
| mm4_intergenic_Nptx2|Gm6272_chr5_144594254 |
ACCAGTGAGCCATCAACCAAGGAACCTTGGGATAAAGTCAACGCCCCTGGTGGCACCATCTGCTCTGGGCATAAGTGGGA CAACACCTGAACCTGGGCATGGCATTTC |
| mm4_exon_Zkscan5_chr5_145207599 | TGCCACAAGTCCCGAAGTACTTCCTCAGAAGATGGTACCACCCGGAGCCACGCAGGAGTCCTTCAGTCACC |
| mm4_intergenic_Gm23117|Gm5083_chr13_44077575 |
GAACAGTCGATGGAGACCCCTCTGAGAAGATGGAGCCCTAGGCTAAAAAGATGAAAGAGACTATATCCCAGCAGTTAAAA TGAAGAGGA |
| mm4_intergenic_Gm25011|Gm23680_chr10_12154646 |
CTGGTTGCTTTTGCCCACTCCTCTTTCTCTCGACACAGCATAATTTCCCAGCATGCCTTGGAGCACCATGTCCTCTGGAA TACTTCTAAACTGATGGCAGCTGAGATGT |
| mm3_intergenic_mmu-mir-6393|Zdhhc25_chr15_88202803 |
AGTGAAAGCAACTTCACCCACACTGGGGCACAATCTTCTCAGAGATAGACCCCTATACAGCCATTGTCCTATTTTTCTCT ATGAGTGAGGTCCCAGTGCA |
| mm4_intron_Ccser1_chr6_62231285 |
TCATCTTGCTCCCTACAAGATTCATCTTGTTCTTATGGAACATTCTCTAGCAACTCTACCTTAAGTTGGTCACCATCTTC TCTGATCTTTCTCATACACAACAGCCTTCGG |
| mm4_intergenic_Gm24751|Angpt1_chr15_42331999 |
GGCACTTTTGAATGCAGAGGAGTCTTTGATCTAGAAATGGAGTCCCCAGAGGGACCCCATCTTCTCAGAAGGAAAGTTCT GACTTGCTTAATGGTGGTGAAAGTTTGTACAGC |
| mm4_intron_Tmprss15_chr16_78968397 |
TGGGAGCATTGTAGACTATAAGAGAAAAGAAGCTTTTATTAGTTAAGTAGACATTTAAATCTATATACCAGATATGAAAT TCAGAAGAGTTGAAATCCACAGAAGAAAATGCCCCACGGTGTTCAAAATAGGGCTGTGCT |
| mm4_intron_Spag1_chr15_36187337 |
TGCAAGCACTTAGAGACCCCTCCACTCCCTGGGGCACATGCTTCTCTGGTTTTCATTCTTTGTACTTGGTCCCTATTTAG TCTTGCAACTCTCCTTTTTACCTCATGAGTTTCATGAAGACGGGGTCTGTTGGAG |
| mm4_intron_Rcan2_chr17_43909877 |
GAACATTCTGCCAGCAAGCCAGGGTCACCCAGAAAAGCTGGTACCCCACTGCTGTCCACTGAGATCCTGGTGACACTTCT TAATAGGTGACTTTACTAGGAATTTGGAGGGAAAGGCTGCT |
| mm4_intron_Mad1l1_chr5_140165615 |
ACGATCCAAGTGCACAGTGTGCTGAGCCACCGCCTGGGTCACCAGCTTCTCTGGAGCCTCATTAGAAACAGCCAACACAT TCCACACCCACATCAGTCAGGCTT |
| mm4_intron_Dnah9_chr11_66142008 |
GTGGAGCAGAGCATGAGGAAGAAGATTGGTATCCTGCTGTCCTGGGAAGCTGCTGCTCCACGGGCTGCGCATGCTCCGCA GACTGCGCACCTTTTACCAGCGCACACCACATGATCGCACACT |
| mm4_intron_Dennd3_chr15_73527245 |
GAGCTGACTTTCCTGGGGACTGCCTGACAAGCTGGGGCCCCATGGGTACTTTCTTAGCCCCATGAAAACAGCACGATCTC CACTCAACCA |
| mm4_intron_Calb2_chr8_110150725 |
ATGTCAGGGACAGAACTGCCCGGGTCTGTGGCAAAGAGTCCCAGAGAACCTGGTTCCCCTCGGTCTTTGTACTATATTTG TGTTTCATATATATATTTCGAATTTTGCTCCTTTGACTTTTCTGCCCATCACACCTGG |