CRISPOR

DNA break repair outcome predictions

Guide: GCAATGGGGCTTCCTCCCAG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCTCGCATCACAAGGTGA`	(Wild-type sequence)
298	`AAAGAAGAGGGCAATGGGGC--------------GGTGCGGCACAGAGGAGCGCTCGCAT`	`14 bp deleted → frameshift`
271	`AAAGAAGAGGGCAATGGGGCTTCCTCCCAG--GCGGTGCGGCACAGAGGAGCGCTCGCAT`	`2 bp deleted → frameshift`
224	`AAAGAAGAGGGCAATGGGGCTTCCTCCCAG----------------AGGAGCGCTCGCAT`	`16 bp deleted → frameshift`
199	`AAAGAAGAGGGCAATGGGGC----------------------ACAGAGGAGCGCTCGCAT`	`22 bp deleted → frameshift`
199	`AAAGAAGAGGGC----------------------GGTGCGGCACAGAGGAGCGCTCGCAT`	`22 bp deleted → frameshift`
188	`AAAGAAGAGGGCAATGGG---------------CGGTGCGGCACAGAGGAGCGCTCGCAT`	`15 bp deleted → no frameshift`
179	`AAAGAAGAGGGCAATGG----------------CGGTGCGGCACAGAGGAGCGCTCGCAT`	`16 bp deleted → frameshift`
170	`AAAGAAGAGGGCAATGGGG-----------------TGCGGCACAGAGGAGCGCTCGCAT`	`17 bp deleted → frameshift`
162	`AAAGAAGAGGGCAATGGG------------------TGCGGCACAGAGGAGCGCTCGCAT`	`18 bp deleted → no frameshift`
158	`AAAGAAGAGG-----------------------CGGTGCGGCACAGAGGAGCGCTCGCAT`	`23 bp deleted → frameshift`
156	`AAAGAAGAGGGCA------------------------------CAGAGGAGCGCTCGCAT`	`30 bp deleted → no frameshift`
154	`AAAGAAGAGGGCAATGGGGC-------------------GGCACAGAGGAGCGCTCGCAT`	`19 bp deleted → frameshift`
154	`AAAGAAGAGGGCAATGG-------------------TGCGGCACAGAGGAGCGCTCGCAT`	`19 bp deleted → frameshift`
149	`AAAGAAGAGGGCAATGGGGCTTCCTCCCA--------------CAGAGGAGCGCTCGCAT`	`14 bp deleted → frameshift`
126	`AAAGAAGAGGGCAATGGG-----------------------CACAGAGGAGCGCTCGCAT`	`23 bp deleted → frameshift`
122	`AAAGAAGAGGGCAATGGGGCTTCCTCCCAG------------------GAGCGCTCGCAT`	`18 bp deleted → no frameshift`
120	`AAAGAAGAGGGCAATGG------------------------CACAGAGGAGCGCTCGCAT`	`24 bp deleted → no frameshift`
114	`AAAGAAGAGGG-------------------------TGCGGCACAGAGGAGCGCTCGCAT`	`25 bp deleted → frameshift`
113	`AAAGAAGAGG---------------------------------------AGCGCTCGCAT`	`39 bp deleted → no frameshift`
111	`AAAGAAGAGGGCAATGGGGCTTCCTC------------------------------GCAT`	`30 bp deleted → no frameshift`
109	`AAAGAAGAGG--------------------------TGCGGCACAGAGGAGCGCTCGCAT`	`26 bp deleted → frameshift`
105	`AAAGAAGAGGGCAATGGGGCTTCCTCCCAG---------------------CGCTCGCAT`	`21 bp deleted → no frameshift`
103	`AAAGAAGAGGGC---------------------------GGCACAGAGGAGCGCTCGCAT`	`27 bp deleted → no frameshift`
99	`AAAGAAGAGGGCAATG----------------------CGGCACAGAGGAGCGCTCGCAT`	`22 bp deleted → frameshift`
91	`AAAGAAGAGGGCAATGGGGCT----------------------------------CGCAT`	`34 bp deleted → frameshift`
89	`AAAGAAGAGGGCAATGGGG------------------------------AGCGCTCGCAT`	`30 bp deleted → no frameshift`
86	`AAAGAAG-------------------------GCGGTGCGGCACAGAGGAGCGCTCGCAT`	`25 bp deleted → frameshift`
84	`AAAGAAGAGGGCAATGGG-------------------------------AGCGCTCGCAT`	`31 bp deleted → frameshift`
84	`AAAGAAGAGG-------------------------------CACAGAGGAGCGCTCGCAT`	`31 bp deleted → frameshift`
80	`AAAGAAGAGGGCAATGGGGC--------------------------------GCTCGCAT`	`32 bp deleted → frameshift`
80	`AAAGAAGAGGGCAATGG--------------------------------AGCGCTCGCAT`	`32 bp deleted → frameshift`
74	`AAAG----------------------------GCGGTGCGGCACAGAGGAGCGCTCGCAT`	`28 bp deleted → frameshift`
66	`AAAGAAGAGGGCAATGGGGCTTCCTCCCA------------------------------T`	`30 bp deleted → no frameshift`
61	`AAAGAAGAG------------------------------------------CGCTCGCAT`	`42 bp deleted → no frameshift`
60	`AAAGAAGAGGGCA--------------------------------GAGGAGCGCTCGCAT`	`32 bp deleted → frameshift`
60	`AAAGAAGAGGGCAATGGGGC--------------------------------------AT`	`38 bp deleted → frameshift`
60	`AAAGAAGAGGG--------------------------------------AGCGCTCGCAT`	`38 bp deleted → frameshift`
57	`AAAGAAGAGGGCAATGGGGCTTC---------------------------------GCAT`	`33 bp deleted → no frameshift`
54	`AAAGAAGAGGGC----------------------------------------GCTCGCAT`	`40 bp deleted → frameshift`
50	`AAAGAAGAGGGCA----------------------------------------------T`	`46 bp deleted → frameshift`
48	`AAAGAAGAGGGC------------------------------------------TCGCAT`	`42 bp deleted → no frameshift`
48	`AAAGA------------------------------------------GGAGCGCTCGCAT`	`42 bp deleted → no frameshift`
47	`AAAGAAGAG-------------------------------------AGGAGCGCTCGCAT`	`37 bp deleted → frameshift`
38	`AAAGAAG-----------------------------------------GAGCGCTCGCAT`	`41 bp deleted → frameshift`
33	`AAAGAAG--------------------------------------------CGCTCGCAT`	`44 bp deleted → frameshift`
33	`AAAG--------------------------------------------GAGCGCTCGCAT`	`44 bp deleted → frameshift`
31	`AAAGA---------------------------------------------GCGCTCGCAT`	`45 bp deleted → no frameshift`
28	`AAAG-----------------------------------------------CGCTCGCAT`	`47 bp deleted → frameshift`
21	`AAAGAAGAGGGCAAT---------------------------------------------`	`45 bp deleted → no frameshift`

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