CRISPOR

DNA break repair outcome predictions

Guide: TCCTTTCGGCTCTCCATCCC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CATCCAGAAACATAACCATGTCCTTTCGGCTCTCCATCCCCGGACTCTCCAGAGGGGGGGAGGGGGAAAACCCCCAAGTA`	(Wild-type sequence)
519	`CATAACCATGTCCTTTCGGCTCTCCA---------------GAGGGGGGGAGGGGGAAAA`	`15 bp deleted → no frameshift`
298	`CATAACCATGTCCTTTCGG--------------ACTCTCCAGAGGGGGGGAGGGGGAAAA`	`14 bp deleted → frameshift`
288	`CATAACCATGTCCTTTCGGCTCTCCATCC-----------AGAGGGGGGGAGGGGGAAAA`	`11 bp deleted → frameshift`
261	`CATAACCATGTCCTTTCGGCTCTC-------------TCCAGAGGGGGGGAGGGGGAAAA`	`13 bp deleted → frameshift`
242	`CATAACCATGTCCTTTCGGCTCTCCATCCC----------AGAGGGGGGGAGGGGGAAAA`	`10 bp deleted → frameshift`
213	`CATAACCATGTCCTTTCGGCTC-----------------CAGAGGGGGGGAGGGGGAAAA`	`17 bp deleted → frameshift`
191	`CATAACCATGTCCTTTCGGCTCTCCATC---------TCCAGAGGGGGGGAGGGGGAAAA`	`9 bp deleted → no frameshift`
129	`CATAACCATGTCC---------------------------AGAGGGGGGGAGGGGGAAAA`	`27 bp deleted → no frameshift`
110	`CATAACCATGTCCTTTC--------------------TCCAGAGGGGGGGAGGGGGAAAA`	`20 bp deleted → frameshift`
109	`CATAACCATGTCCTTTCGG--------------------------GGGGGAGGGGGAAAA`	`26 bp deleted → frameshift`
103	`CATAACCATGTCCTTTCGG---------------------------GGGGAGGGGGAAAA`	`27 bp deleted → no frameshift`
99	`CATAACCATGTCCTTTC----------------------CAGAGGGGGGGAGGGGGAAAA`	`22 bp deleted → frameshift`
99	`CATAACCATGTCCT----------------------CTCCAGAGGGGGGGAGGGGGAAAA`	`22 bp deleted → frameshift`
98	`CATAACCATGTCCTTTCGG----------------------------GGGAGGGGGAAAA`	`28 bp deleted → frameshift`
96	`CATAACCA---------------------------------GAGGGGGGGAGGGGGAAAA`	`33 bp deleted → no frameshift`
94	`CATAACCATGTCCTTTCGG-----------------------------GGAGGGGGAAAA`	`29 bp deleted → frameshift`
90	`CATAACCATGTCCT------------------------CCAGAGGGGGGGAGGGGGAAAA`	`24 bp deleted → no frameshift`
89	`CATAACCATGTCCTTTCGG------------------------------GAGGGGGAAAA`	`30 bp deleted → no frameshift`
86	`CATAACCATGTC-------------------------TCCAGAGGGGGGGAGGGGGAAAA`	`25 bp deleted → frameshift`
84	`CATAACCATGTCCTTTCGG-------------------------------AGGGGGAAAA`	`31 bp deleted → frameshift`
73	`CATAACCATGTCCTTTCGG----------------------------------GGGAAAA`	`34 bp deleted → frameshift`
70	`CATAAC-----------------------------TCTCCAGAGGGGGGGAGGGGGAAAA`	`29 bp deleted → frameshift`
69	`CATAACCATGTCCTTTCGG-----------------------------------GGAAAA`	`35 bp deleted → frameshift`
66	`CATAACCATGTCCTTTCGG------------------------------------GAAAA`	`36 bp deleted → no frameshift`
62	`CATAACCATGTCCTTTCGG-------------------------------------AAAA`	`37 bp deleted → frameshift`
42	`CA---------------------------------------GAGGGGGGGAGGGGGAAAA`	`39 bp deleted → no frameshift`
14	`CATAA-----------------------------------------------------AA`	`53 bp deleted → frameshift`
13	`CATAA------------------------------------------------------A`	`54 bp deleted → no frameshift`
12	`CATAA-------------------------------------------------------`	`55 bp deleted → frameshift`

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