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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314084_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314084_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm4_intergenic_Tcf7l2|Gm22271_chr19_55992149_F | TCGTCGGCAGCGTCAATAATGCAGCCAAGTGCCC | 59.1 | 0.56 |
| mm4_intergenic_Tcf7l2|Gm22271_chr19_55992149_R | GTCTCGTGGGCTCGGTAAATGCCGGGGCGTAAAGA | 59.7 | 0.56 |
| mm4_intron_Cdc14a_chr3_116337032_F | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intron_Cdc14a_chr3_116337032_R | Primer3: not found at this Tm | N.d. | 0.53 |
| mm4_intergenic_Thsd4|Lrrc49_chr9_60542827_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Thsd4|Lrrc49_chr9_60542827_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm3_intron_Clpb_chr7_101739706_F | TCGTCGGCAGCGTCTCATGAACAAAAGCTGCTGCC | 60.0 | 0.34 |
| mm3_intron_Clpb_chr7_101739706_R | GTCTCGTGGGCTCGGCTTGCCAAGGACAGCTGAGA | 59.9 | 0.34 |
| mm4_exon_Phactr1_chr13_43077790_F | TCGTCGGCAGCGTCTATGCAGCCCAGAAGAGCAG | 59.8 | 0.31 |
| mm4_exon_Phactr1_chr13_43077790_R | GTCTCGTGGGCTCGGGCATCTGGGATGGTAGGACG | 59.9 | 0.31 |
| mm4_exon_Paqr4/Pkmyt1_chr17_23737946_F | TCGTCGGCAGCGTCGCTACACCTGACAGTGCAGT | 59.9 | 0.25 |
| mm4_exon_Paqr4/Pkmyt1_chr17_23737946_R | GTCTCGTGGGCTCGGCCCATCATCCACTGCACTCT | 59.4 | 0.25 |
| mm2_exon_Slc10a4_chr5_73007037_F | TCGTCGGCAGCGTCGACGCTTGACCAGACAGACA | 59.9 | 0.24 |
| mm2_exon_Slc10a4_chr5_73007037_R | GTCTCGTGGGCTCGGTGTTGTCCAGGCTGTCCATG | 60.2 | 0.24 |
| mm4_intron_Tle3_chr9_61415518_F | TCGTCGGCAGCGTCAACCGTGGTCAAGCATGTCT | 59.8 | 0.20 |
| mm4_intron_Tle3_chr9_61415518_R | GTCTCGTGGGCTCGGCTCACAAACCACTTGCCTGC | 59.9 | 0.20 |
| mm4_intergenic_Gm5633|Gm22908_chr12_21784274_F | TCGTCGGCAGCGTCCGCCTTGTAGTCCTCAAGGG | 60.1 | 0.18 |
| mm4_intergenic_Gm5633|Gm22908_chr12_21784274_R | GTCTCGTGGGCTCGGCCCTTTGTTCAAGTCCCCGA | 59.8 | 0.18 |
| mm4_intergenic_Gm5633|Gm22908_chr12_21778608_F | TCGTCGGCAGCGTCGAGATCTGGTCCGGCATCG | 60.0 | 0.18 |
| mm4_intergenic_Gm5633|Gm22908_chr12_21778608_R | GTCTCGTGGGCTCGGCCCTTTGTTCAAGTCCCCGA | 59.8 | 0.18 |
| mm4_intergenic_5730507C01Rik|Gm3944_chr12_18610355_F | TCGTCGGCAGCGTCCGCTCCGCTTTGTTCAAGTC | 60.1 | 0.18 |
| mm4_intergenic_5730507C01Rik|Gm3944_chr12_18610355_R | GTCTCGTGGGCTCGGCACGAATTCGGACACGGAGA | 60.1 | 0.18 |
| mm4_intergenic_Mir149|Gpc1_chr1_92851030_F | TCGTCGGCAGCGTCCCCTTAAACCCCACCACTCA | 59.2 | 0.18 |
| mm4_intergenic_Mir149|Gpc1_chr1_92851030_R | GTCTCGTGGGCTCGGAGGCACAACAAGGTACAGGG | 59.8 | 0.18 |
| mm4_intron_Bend6_chr1_33907448_F | TCGTCGGCAGCGTCTCAGGGTTCTTTGACCGTGG | 59.8 | 0.18 |
| mm4_intron_Bend6_chr1_33907448_R | GTCTCGTGGGCTCGGCCATCTCCAAGCTGGTCCAA | 59.6 | 0.18 |
| mm3_intron_Gm5868_chr5_72587393_F | TCGTCGGCAGCGTCAAGGAGCAGGGTGGTGTTG | 59.8 | 0.17 |
| mm3_intron_Gm5868_chr5_72587393_R | GTCTCGTGGGCTCGGGACGCTTGACCAGACAGACA | 59.9 | 0.17 |
| mm3_exon_Gjd3_chr11_98982586_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_exon_Gjd3_chr11_98982586_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Mettl20|Amn1_chr6_149151260_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Mettl20|Amn1_chr6_149151260_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Ptprn2_chr12_117041620_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intron_Ptprn2_chr12_117041620_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_exon_Gpd1/Gm16537_chr15_99723554_F | TCGTCGGCAGCGTCCCTTGCTGTCCTCTGGGAAG | 60.0 | 0.12 |
| mm4_exon_Gpd1/Gm16537_chr15_99723554_R | GTCTCGTGGGCTCGGGGTTCCCAGTGTGTGTGAGG | 60.5 | 0.12 |
| mm4_intron_Dock1_chr7_134671396_F | TCGTCGGCAGCGTCAGTCCCGCTTTCCTCAATGG | 60.0 | 0.10 |
| mm4_intron_Dock1_chr7_134671396_R | GTCTCGTGGGCTCGGCCTGAGGCCTAGACAGACCT | 60.0 | 0.10 |
| mm4_intron_Smyd2_chr1_189906577_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Smyd2_chr1_189906577_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm11746|Slc24a3_chr2_145161441_F | TCGTCGGCAGCGTCACCCACAAACCTGAGACAGC | 60.1 | 0.09 |
| mm4_intergenic_Gm11746|Slc24a3_chr2_145161441_R | GTCTCGTGGGCTCGGAACTCAAACCCAGCACAGCT | 60.1 | 0.09 |
| mm4_intergenic_Gm14262|Gm22704_chr2_168318516_F | TCGTCGGCAGCGTCCATCGGGACTGCCTAGTGTC | 59.8 | 0.09 |
| mm4_intergenic_Gm14262|Gm22704_chr2_168318516_R | GTCTCGTGGGCTCGGACAAGTTGTCCGTGCACAGA | 60.1 | 0.09 |
| mm4_exon_Tchp_chr5_114714743_F | TCGTCGGCAGCGTCGAGTCGAGGAGGAGAGGAGG | 60.1 | 0.08 |
| mm4_exon_Tchp_chr5_114714743_R | GTCTCGTGGGCTCGGCGCTCACCTCCATCTCCTTC | 59.8 | 0.08 |
| mm4_intergenic_Nrg3|Gm10713_chr14_39473595_F | TCGTCGGCAGCGTCGCTCTCCGGGAGAAACCAAT | 59.7 | 0.07 |
| mm4_intergenic_Nrg3|Gm10713_chr14_39473595_R | GTCTCGTGGGCTCGGGGGGTAGCTTGCTGGAGTTT | 59.9 | 0.07 |
| mm3_intergenic_Ppip5k2|Ppip5k2/Gin1_chr1_97769806_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm3_intergenic_Ppip5k2|Ppip5k2/Gin1_chr1_97769806_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_Barx1|Phf2_chr13_48782936_F | TCGTCGGCAGCGTCCCTCTGTCTGGGCATGTGTT | 59.9 | 0.06 |
| mm4_intergenic_Barx1|Phf2_chr13_48782936_R | GTCTCGTGGGCTCGGCCTCCCGACTACTGCCTAGG | 60.8 | 0.06 |
| mm4_exon_1700024N20Rik/Prosc_chr8_27042685_F | TCGTCGGCAGCGTCAATGTTGAGAGGTGGCAGCA | 59.8 | 0.06 |
| mm4_exon_1700024N20Rik/Prosc_chr8_27042685_R | GTCTCGTGGGCTCGGCTTCCAGCCCTCCCCAGT | 60.6 | 0.06 |
| mm4_intergenic_Plekha7|Rps13_chr7_116319082_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Plekha7|Rps13_chr7_116319082_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_exon_Col9a3_chr2_180598212_F | TCGTCGGCAGCGTCGGCTGAATGAGGGGCTTGT | 60.0 | 0.05 |
| mm3_exon_Col9a3_chr2_180598212_R | GTCTCGTGGGCTCGGTCCGGTCATGGCTGGCAG | 62.1 | 0.05 |
| mm4_exon_Nrxn2_chr19_6490071_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_exon_Nrxn2_chr19_6490071_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm21859|Litaf_chr16_10920049_F | TCGTCGGCAGCGTCACAGGCTCTGGTCTCCATCA | 60.2 | 0.05 |
| mm4_intergenic_Gm21859|Litaf_chr16_10920049_R | GTCTCGTGGGCTCGGTGGTTGGTGTTCTGTGCTGT | 60.0 | 0.05 |
| mm4_exon_Cdr2_chr7_120958429_F | TCGTCGGCAGCGTCTGGAGCAGCCAGGAACATTT | 59.8 | 0.05 |
| mm4_exon_Cdr2_chr7_120958429_R | GTCTCGTGGGCTCGGATGGTGTGGAGAAGCTGGTG | 59.9 | 0.05 |
| mm4_intron_Rrbp1_chr2_144010457_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Rrbp1_chr2_144010457_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Nucks1_chr1_131910609_F | TCGTCGGCAGCGTCCAGGGACCTCAAACCAGCAG | 60.6 | 0.04 |
| mm4_exon_Nucks1_chr1_131910609_R | GTCTCGTGGGCTCGGAGTCGAGGAGCCGAGGAC | 60.4 | 0.04 |
| mm4_exon_Scap_chr9_110333625_F | TCGTCGGCAGCGTCCTCGTGCCCGGGAGTTCC | 62.4 | 0.04 |
| mm4_exon_Scap_chr9_110333625_R | GTCTCGTGGGCTCGGCAGCTCCAGACTGCGGTC | 60.1 | 0.04 |
| mm4_intergenic_Auh|n-R5s54_chr13_52945932_F | TCGTCGGCAGCGTCTGTGCTTGAATGGCAGACTG | 58.7 | 0.04 |
| mm4_intergenic_Auh|n-R5s54_chr13_52945932_R | GTCTCGTGGGCTCGGAGCCATTCTTCACGTCCCTG | 60.0 | 0.04 |
| mm4_intron_Pllp_chr8_94695616_F | TCGTCGGCAGCGTCTGGCAGACACGTGGTTTCTT | 60.1 | 0.03 |
| mm4_intron_Pllp_chr8_94695616_R | GTCTCGTGGGCTCGGAGGGCAGAACACCACATCAG | 59.9 | 0.03 |
| mm4_intergenic_Hoxc6|Hoxc5_chr15_103011994_F | TCGTCGGCAGCGTCCTTGCCAGCAGAGCTCTCG | 60.8 | 0.03 |
| mm4_intergenic_Hoxc6|Hoxc5_chr15_103011994_R | GTCTCGTGGGCTCGGTCTCAGAACCCCATCTCGGT | 59.9 | 0.03 |
| mm4_intergenic_Gm22853|Gm25611_chr19_35691183_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm22853|Gm25611_chr19_35691183_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm16578|Cplx2_chr13_54361608_F | TCGTCGGCAGCGTCCCCACGGAACAAACAAGAGG | 59.0 | 0.03 |
| mm4_intergenic_Gm16578|Cplx2_chr13_54361608_R | GTCTCGTGGGCTCGGGCCCTGGATAGATGTCAGCC | 59.9 | 0.03 |
| mm4_intergenic_Gm15927|Cnot3_chr7_3645181_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm15927|Cnot3_chr7_3645181_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Mpp2|Ppy_chr11_102097179_F | TCGTCGGCAGCGTCCACCTGACCATGCTGTCTGT | 59.9 | 0.02 |
| mm4_intergenic_Mpp2|Ppy_chr11_102097179_R | GTCTCGTGGGCTCGGCTAGGCAACCCAGAGCCAG | 59.7 | 0.02 |
| mm4_intergenic_Atp2b1|Poc1b_chr10_99083619_F | TCGTCGGCAGCGTCGCATTGTCCTGTCGAGCTCT | 60.1 | 0.01 |
| mm4_intergenic_Atp2b1|Poc1b_chr10_99083619_R | GTCTCGTGGGCTCGGATACCCAGCCTTGCTCTCCT | 60.3 | 0.01 |
| mm4_intron_Slc6a12_chr6_121344336_F | TCGTCGGCAGCGTCGTGGTTTCAGCCCTCTCTCC | 60.0 | 0.01 |
| mm4_intron_Slc6a12_chr6_121344336_R | GTCTCGTGGGCTCGGAAGGCAGGCAAAAGGAGAGG | 60.2 | 0.01 |
| mm4_exon_Ccm2l_chr2_153071924_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Ccm2l_chr2_153071924_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Ptges2_chr2_32396831_F | TCGTCGGCAGCGTCCAGACTCAGAACTGGAGGCG | 60.1 | 0.00 |
| mm4_intron_Ptges2_chr2_32396831_R | GTCTCGTGGGCTCGGCAGGAAAGGAGCATGGGAGG | 60.1 | 0.00 |
| mm4_intergenic_Zfp770|Gm25103_chr2_114244885_F | TCGTCGGCAGCGTCCCTCCATCCTTCCCCTGAGA | 60.0 | 0.00 |
| mm4_intergenic_Zfp770|Gm25103_chr2_114244885_R | GTCTCGTGGGCTCGGGTTGGAGTAGTGGAAGGAGCC | 60.0 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314084 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_intergenic_Tcf7l2|Gm22271_chr19_55992149 |
AATAATGCAGCCAAGTGCCCGCTGTCCCCTGCAGATCGTGGCTCTGTGCGCCATTACTTCCCAAATTGTCCTGCTGAAAG AATTCGCTTGTTTACATCTCTTTACGCCCCGGCATTTA |
| mm3_intron_Clpb_chr7_101739706 |
TCATGAACAAAAGCTGCTGCCTCCTGCAGACTGTGGCTCTGCCCCAGACGTCAGCAGTATAATTACAACTGCTGAATTTA GCCTAGACCTGAGCTTGGCTTCCTCAGTCTCAGCTGTCCTTGGCAAG |
| mm4_exon_Phactr1_chr13_43077790 | TATGCAGCCCAGAAGAGCAGCCAGCAGGCCGTGGCCCAGCACCACCACACCGTCCTACCATCCCAGATGC |
| mm4_exon_Paqr4/Pkmyt1_chr17_23737946 |
GCTACACCTGACAGTGCAGTGTAACCCATCAGGGCAGCTGGGCGAAGCCACGGTCTGCAGGCCAGAGTGCAGTGGATGAT GGG |
| mm2_exon_Slc10a4_chr5_73007037 |
GACGCTTGACCAGACAGACAGGTAAAAGGGACCACAGGACCGAGGGAGGAGGTGTGGCCAGGGACAGCCCCTGCAGACCG TGCCTCGGCGACAGAGCCATGGACAGCCTGGACAACA |
| mm4_intron_Tle3_chr9_61415518 |
AACCGTGGTCAAGCATGTCTGAGGATGTTTCCACGCGGGGCAGACACAGCTGGGTTGGGTCCTGCTGCACAAGGCAGCGA GCCACAGTCTGAGGGCTCCTTTGCTTGCCTTGCAGGCAAGTGGTTTGTGAG |
| mm4_intergenic_Gm5633|Gm22908_chr12_21784274 |
CGCCTTGTAGTCCTCAAGGGTCTCGGCCACGAATTCCGACACGGAGATCTGGTCCGGCATCGCCAGGGCGCCGGGGCGCG GACTGCGGGCGCGTCAGTCGCCCAGGGCTCGGGCCGCCGCTCGGGGACTTGAACAAAGGG |
| mm4_intergenic_Gm5633|Gm22908_chr12_21778608 |
GAGATCTGGTCCGGCATCGCCAGGGCGCCGGGGCGCGGACTGCGGGCGCGTCAGTCGCCCAGGGCGCGGGCTCCGACCCC GCCGCCGCTCGGGGACTTGAACAAAGGG |
| mm4_intergenic_5730507C01Rik|Gm3944_chr12_18610355 |
CGCTCCGCTTTGTTCAAGTCCCCGAGCGGCGGCGGGGTCGGAGCCCGCGCCCTGGGCGACTGAGGCGCCCGCAGTCCGCG CCCCGGCGCCGTGGCGATGCCGGACCAGATCTCCGTGTCCGAATTCGTG |
| mm4_intergenic_Mir149|Gpc1_chr1_92851030 |
CCCTTAAACCCCACCACTCAAAGAGAGAGATGTGGCATATCACCTCAGCCCAGCAGACCATGGCCCGGAGCAGAGCAAAT AGAAGGCCCTGCTGGGCCCTGTACCTTGTTGTGCCT |
| mm4_intron_Bend6_chr1_33907448 |
TCAGGGTTCTTTGACCGTGGCAGAGCTCACGTCTGGGCTGAGAATCAGCACCAAGTTTGGGGGTGGTAGGGTGCAAGCCT GCAGACCGCGGCGTGCCGCCTTTCCTAAGATTGGACCAGCTTGGAGATGG |
| mm3_intron_Gm5868_chr5_72587393 |
AAGGAGCAGGGTGGTGTTGTCCAGGCCGTCCATGGCTCTGTCGCCGAGGCGCGGTCTGCAGGGGCTGTCCCTGGCCACAC CTCCTCCCTCGGTCCTGTGGTCCCTTTTACCTGTCTGTCTGGTCAAGCGTC |
| mm4_exon_Gpd1/Gm16537_chr15_99723554 |
CCTTGCTGTCCTCTGGGAAGTGTAGAACTAAGCCCCAGTGGTGCCTGCTCCAGGGGTGGGGTTGGGGGAAGGGAAGGCGC CAAGTCAAGGGCTGCCGGTTGCTGCCTCACACACACTGGGAACC |
| mm4_intron_Dock1_chr7_134671396 |
AGTCCCGCTTTCCTCAATGGCCAGACAGTCCCAGAGCCCTGCAGACCTTGTCTCGCCCCCCAGCTAGCCCACATCAAAAG TCAGGTCTGTCTAGGCCTCAGG |
| mm4_intergenic_Gm11746|Slc24a3_chr2_145161441 |
ACCCACAAACCTGAGACAGCACAGGATAGCGTGGCGCCAACCCACTGTCTTCAGGTGTTTTTCATGATGAAGACTTTAGG TAAGTTGCGTATTTACCTGACAGCTGTGCTGGGTTTGAGTT |
| mm4_intergenic_Gm14262|Gm22704_chr2_168318516 |
CATCGGGACTGCCTAGTGTCATGGCTTTGGTTCTGCCTGCGTCCCACGACGGCCCCTGCAGCCCGTGGCTCCCCGCTCAT CCTCTTCTGTGCACGGACAACTTGT |
| mm4_exon_Tchp_chr5_114714743 |
GAGTCGAGGAGGAGAGGAGGCAGCTAGAGGGGCGCCTGCAGGCCGAGGCGCTGCGCCAGCAGATGGAGGAGCTGAAGCAG AAGGAGATGGAGGTGAGCG |
| mm4_intergenic_Nrg3|Gm10713_chr14_39473595 |
GCTCTCCGGGAGAAACCAATCCTAGAGCAGCGCGGAGGGCGAGCGACCGGGCTCGCCTCGCTCCCGCCGCCCGCGGCTCT GCGCCGCTACTGCCGCCGCCACCGTGAGTCAAACTCCAGCAAGCTACCCC |
| mm4_intergenic_Barx1|Phf2_chr13_48782936 | CCTCTGTCTGGGCATGTGTTAGACCTTGAAGCCCAGGGGCGGAGCCGCGGGCTGCAGGCCTAGGCAGTAGTCGGGAGG |
| mm4_exon_1700024N20Rik/Prosc_chr8_27042685 |
AATGTTGAGAGGTGGCAGCATGACGGCGGAGCTGGGAGTCGGGTTCGCGCTGCGGGCCGTGAACGAGCGAGTCCAGCAGA GTGTGGCGCGGCGGCCGCGGGTGAGGACCGGCGGAAGGGCACTGGGGAGGGCTGGAAG |
| mm3_exon_Col9a3_chr2_180598212 |
GGCTGAATGAGGGGCTTGTGCGCGGGTGGGGCGGGGCAGGGCGGGGCACACGGCCTCGCGCCGCCCCCAGCCCCTCTCCC CACCCTGCTGCCCGTCGCTCGGCGCCTGCCGCTCAGAGCGCCTGCCAGCCATGACCGGA |
| mm4_intergenic_Gm21859|Litaf_chr16_10920049 |
ACAGGCTCTGGTCTCCATCAACAAGAAAAGGTTGGGAGAGCACAGAGCAAATGTCCTGCAGACATTGGCTTGGGGCCAAA TGTCAGGACTTCACAACAGCACAGAACACCAACCA |
| mm4_exon_Cdr2_chr7_120958429 |
TGGAGCAGCCAGGAACATTTCCTCCAGCAGACTCTGGCTAGGCTCCTTGAAAGGAACAAACAGAGAGTCGGGCACCAGCT TCTCCACACCAT |
| mm4_exon_Nucks1_chr1_131910609 |
CAGGGACCTCAAACCAGCAGCCGCCGACTCTTGAGAAGCCAGCAGCCCGGGGCTCGGCAGCANNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNAGTGCGCGGGGAGGGGGGTCCTGGTCCTCGGCTCCTCGACT |
| mm4_exon_Scap_chr9_110333625 |
CTCGTGCCCGGGAGTTCCGGGATGCGGGCGAGCCCGGGCAGGGGCGCGCGGGCGTGGGCGCTGTCCAGCAGCCCGAGGCG CGGCTCCGGAGACCGCAGTCTGGAGCTG |
| mm4_intergenic_Auh|n-R5s54_chr13_52945932 |
TGTGCTTGAATGGCAGACTGTTTACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAAGCAAACCAGCAGTGGTT GGCTCGGCGCCATCCCTTCTCAGGGACGTGAAGAATGGCT |
| mm4_intron_Pllp_chr8_94695616 |
TGGCAGACACGTGGTTTCTTGCCTCTGCGAATCTCCACAGCCCAAGGAAGGAGATTCGACCAGCAGACCCTGGGTAGGTG CCCTGGTGGGAGCGCGCGTGTCAGAGCCACTGATGTGGTGTTCTGCCCT |
| mm4_intergenic_Hoxc6|Hoxc5_chr15_103011994 |
CTTGCCAGCAGAGCTCTCGCGCGGCCTGCAGCTTGCAAAGCCCCCAGCCCTCCGCACTCGCCTGCCCTGCAGCCAGGCGC TGAGCCTCTGTCTCCGGGTTCTCCTGACCGAGATGGGGTTCTGAGA |
| mm4_intergenic_Gm16578|Cplx2_chr13_54361608 |
CCCACGGAACAAACAAGAGGATGAACAAGTTAAGAAAGGAAGCAAACATCCTAGAGTGTAGTGTTTAGGAGCAGAGCCAC GGTGTACAGGCTGGGGAGAGGGCTGACATCTATCCAGGGC |
| mm4_intergenic_Mpp2|Ppy_chr11_102097179 |
CACCTGACCATGCTGTCTGTCCCGCCCTCCCCCTCCCCCCACCAGACCGTGGCTCTGCCCTGCGCGTGCGCACTGGCTCT GGGTTGCCTAG |
| mm4_intergenic_Atp2b1|Poc1b_chr10_99083619 |
GCATTGTCCTGTCGAGCTCTGTGGCCTGGGACGCTGACCTCCACTAGGGCCGCTGTTGCACTTGCTTTTGCTCATTCTCT GCCAGGAGACCCTGGCACGGGGCCAGAGAGCAGAGGAGAGCAAGGCTGGGTAT |
| mm4_intron_Slc6a12_chr6_121344336 | GTGGTTTCAGCCCTCTCTCCTCCCTGCTCACCGTGGCTCTGAGCCACCTCTCCTTTTGCCTGCCTT |
| mm4_intron_Ptges2_chr2_32396831 |
CAGACTCAGAACTGGAGGCGTGCTCCAGCCCACCGTGGCTCGGCAGCCCTGTAGACCTTTCATGAGTTGCAGTCTTTAGC CCTTCTCCGCCCTGCCTCCCATGCTCCTTTCCTG |
| mm4_intergenic_Zfp770|Gm25103_chr2_114244885 |
CCTCCATCCTTCCCCTGAGACTAAACACTTACTACTCGCTCTGTCTATGTCTCCCCCTCCAGACAGTGGCACGGCTCCGG CTCCTTCCACTACTCCAAC |