CRISPOR

DNA break repair outcome predictions

Guide: GGCGCCGAGCCACGGTCTGC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACCCCCAGTCCCCTCC`	(Wild-type sequence)
314	`TGCAGGGCTGGGCGCCGAGC----------------GGCCATGCTTGGCCCGGGACCCCC`	`16 bp deleted → frameshift`
313	`TGCAGGGCTGGGCGCCGAGCCACGG-------------CCATGCTTGGCCCGGGACCCCC`	`13 bp deleted → frameshift`
296	`TGCAGGGCTGGGCGCCGAGCCACGGTCTGC------GGCCATGCTTGGCCCGGGACCCCC`	`6 bp deleted → no frameshift`
270	`TGCAGGGCTGGGCGCCGAGCCA-------------------TGCTTGGCCCGGGACCCCC`	`19 bp deleted → frameshift`
268	`TGCAGGGCTGGGCGCCGAGCCACGG--------AGCGGCCATGCTTGGCCCGGGACCCCC`	`8 bp deleted → frameshift`
258	`TGCAGGGCTGGGCGCCGAGCCACGGTCTG---GAGCGGCCATGCTTGGCCCGGGACCCCC`	`3 bp deleted → no frameshift`
255	`TGCAGGGCTGGGCGCCGAGCCACGGTCTGC---------CATGCTTGGCCCGGGACCCCC`	`9 bp deleted → no frameshift`
248	`TGCAGGGCTGGGCGCCGAGCCACGGTCTGC--------------TTGGCCCGGGACCCCC`	`14 bp deleted → frameshift`
190	`TGCAGGGCTGGGCG-----------------------GCCATGCTTGGCCCGGGACCCCC`	`23 bp deleted → frameshift`
180	`TGCAGGGCTGGGCGCC------------------------ATGCTTGGCCCGGGACCCCC`	`24 bp deleted → no frameshift`
166	`TGCAGGGCTGG----------------------AGCGGCCATGCTTGGCCCGGGACCCCC`	`22 bp deleted → frameshift`
163	`TGCAGGGCTGGGC--------------------------CATGCTTGGCCCGGGACCCCC`	`26 bp deleted → frameshift`
154	`TGCAGGGCTGGGCGCCGAGCCACGGTCTGC-------------------CCGGGACCCCC`	`19 bp deleted → frameshift`
148	`TGCAGGGCTGGGCGCCGAGCCACGG----------------------------GACCCCC`	`28 bp deleted → frameshift`
147	`TGCAGGGCTGGGCGCCG--------------------GCCATGCTTGGCCCGGGACCCCC`	`20 bp deleted → frameshift`
141	`TGCAGGGCTGGGCGCCGAGCC-----------------------------CGGGACCCCC`	`29 bp deleted → frameshift`
140	`TGCAGGGCTGGGCGC---------------------GGCCATGCTTGGCCCGGGACCCCC`	`21 bp deleted → no frameshift`
140	`TGCAGGGCTGGG---------------------AGCGGCCATGCTTGGCCCGGGACCCCC`	`21 bp deleted → no frameshift`
128	`TGCAGGGCTGGGCGCCGAGCCACGGTCT-----------------TGGCCCGGGACCCCC`	`17 bp deleted → frameshift`
127	`TGCAGGGC-------------------------------CATGCTTGGCCCGGGACCCCC`	`31 bp deleted → frameshift`
126	`TGCAGGGCTGGGCGCCGAGCCACGG-----------------------CCCGGGACCCCC`	`23 bp deleted → frameshift`
122	`TGCAGGGCTGGGCGCCGAGCCACGGTCTG------------------GCCCGGGACCCCC`	`18 bp deleted → no frameshift`
120	`TGCAGGGCTGGGCGCCGAGC------------------------TTGGCCCGGGACCCCC`	`24 bp deleted → no frameshift`
109	`TGCAGGGCTGGGCGCC----------------------------------CGGGACCCCC`	`34 bp deleted → frameshift`
109	`TGCAGGG--------------------------AGCGGCCATGCTTGGCCCGGGACCCCC`	`26 bp deleted → frameshift`
104	`TGCAGGGCTGGGCGCCG-----------------------------------GGACCCCC`	`35 bp deleted → frameshift`
103	`TGCAGGGCTGG---------------------------CCATGCTTGGCCCGGGACCCCC`	`27 bp deleted → no frameshift`
103	`TGCAGG---------------------------AGCGGCCATGCTTGGCCCGGGACCCCC`	`27 bp deleted → no frameshift`
99	`TGCAGGGCTGGGC------------------------------------CCGGGACCCCC`	`36 bp deleted → no frameshift`
98	`TGCAGGGC----------------------------GGCCATGCTTGGCCCGGGACCCCC`	`28 bp deleted → frameshift`
94	`TGCAGGGCTGGGCGCCGAGCCACGG-----------------------------ACCCCC`	`29 bp deleted → frameshift`
94	`TGCAGGGCTGGGCGC-----------------------------TTGGCCCGGGACCCCC`	`29 bp deleted → frameshift`
89	`TGCAGGGCTGGGCGCCGAGCC------------------------------GGGACCCCC`	`30 bp deleted → no frameshift`
84	`TGCAGGGCTGGGC-------------------------------TTGGCCCGGGACCCCC`	`31 bp deleted → frameshift`
82	`TGCAGGGCT------------------------------------TGGCCCGGGACCCCC`	`36 bp deleted → no frameshift`
80	`TGCAGG--------------------------------CCATGCTTGGCCCGGGACCCCC`	`32 bp deleted → frameshift`
78	`TGCAGGGCTGG-------------------------------------CCCGGGACCCCC`	`37 bp deleted → frameshift`
77	`TGCAGGGC-----------------------------------------CCGGGACCCCC`	`41 bp deleted → frameshift`
76	`TGC---------------------------------GGCCATGCTTGGCCCGGGACCCCC`	`33 bp deleted → no frameshift`
73	`TGCAGGGCTGGG------------------------------------------ACCCCC`	`42 bp deleted → no frameshift`
66	`TGCAG------------------------------CGGCCATGCTTGGCCCGGGACCCCC`	`30 bp deleted → no frameshift`
66	`TG------------------------------GAGCGGCCATGCTTGGCCCGGGACCCCC`	`30 bp deleted → no frameshift`
66	`TGCAGGGCTGGGCGCCGAGCC------------------------------------CCC`	`36 bp deleted → no frameshift`
66	`TGC------------------------------------CATGCTTGGCCCGGGACCCCC`	`36 bp deleted → no frameshift`
64	`TGC-----------------------------------------TTGGCCCGGGACCCCC`	`41 bp deleted → frameshift`
62	`TGCAGGGCTGGGCGCCGAGCC-------------------------------------CC`	`37 bp deleted → frameshift`
60	`TGCAGGGCTGGGCGCCGAGCC--------------------------------------C`	`38 bp deleted → frameshift`
60	`TGCAGGGCTGGGCG--------------------------------------GGACCCCC`	`38 bp deleted → frameshift`
57	`TGCAGGGCTGGGCGCCGAGCCAC---------------------------------CCCC`	`33 bp deleted → no frameshift`
57	`TGCAGGGCTG---------------------------------CTTGGCCCGGGACCCCC`	`33 bp deleted → no frameshift`
57	`TGCAGGG-----------------------------------------------ACCCCC`	`47 bp deleted → frameshift`
56	`TGCAGGGCTGGGCGCCGAGCC---------------------------------------`	`39 bp deleted → no frameshift`
51	`TGCAGGGCTGGGCGCC-----------------------------------------CCC`	`41 bp deleted → frameshift`
51	`TGCAGGGCTGGG-----------------------------------------GACCCCC`	`41 bp deleted → frameshift`
48	`TGCAGGGCTGGGCGCC------------------------------------------CC`	`42 bp deleted → no frameshift`
48	`TGCAGG------------------------------------------CCCGGGACCCCC`	`42 bp deleted → no frameshift`
47	`TGCAGGGCTGGGCGCCGA-------------------------------------CCCCC`	`37 bp deleted → frameshift`
47	`TGCA-------------------------------------TGCTTGGCCCGGGACCCCC`	`37 bp deleted → frameshift`
46	`TGCAGGGCTGGGCGCC-------------------------------------------C`	`43 bp deleted → frameshift`
46	`TGCAGGGCTGG-------------------------------------------ACCCCC`	`43 bp deleted → frameshift`
44	`TGCAGGGCTGGGCGCC--------------------------------------------`	`44 bp deleted → frameshift`
40	`TGCAGGG----------------------------------------------GACCCCC`	`46 bp deleted → frameshift`
40	`TGC----------------------------------------------CCGGGACCCCC`	`46 bp deleted → frameshift`
36	`TGCAGG------------------------------------------------ACCCCC`	`48 bp deleted → no frameshift`
31	`TG---------------------------------------------GCCCGGGACCCCC`	`45 bp deleted → no frameshift`

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