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Contents:

PCR primers for off-targets of GCCTCCCCCGCCACGCTCCC GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314013_F TCGTCGGCAGCGTCAACCGCCAGAGAAGATGGTG 60.0 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314013_R GTCTCGTGGGCTCGGCTGCCTGCTAGTCCATCGAC 60.2 1.00
mm4_intron_Fam186b_chr15_99285113_F TCGTCGGCAGCGTCCCTGGCCTCTTCCTGTTCTC 59.7 0.66
mm4_intron_Fam186b_chr15_99285113_R GTCTCGTGGGCTCGGCAGTGGTGATCTCTTGGCCC 60.3 0.66
mm4_exon_Garnl3_chr2_33009781_F Primer3: not found at this Tm N.d. 0.57
mm4_exon_Garnl3_chr2_33009781_R Primer3: not found at this Tm N.d. 0.57
mm3_intergenic_Tenm4|Gm15414_chr7_96559034_F TCGTCGGCAGCGTCGGGTATCTCCAGGGCATGTG 59.8 0.51
mm3_intergenic_Tenm4|Gm15414_chr7_96559034_R GTCTCGTGGGCTCGGACATTTCGAGCTTCCTGACAGT 59.9 0.51
mm2_intergenic_Mpp5|Gm25225_chr12_78749158_F Primer3: not found at this Tm N.d. 0.46
mm2_intergenic_Mpp5|Gm25225_chr12_78749158_R Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Ddah1|Bcl10_chr3_145902271_F Primer3: not found at this Tm N.d. 0.43
mm4_intergenic_Ddah1|Bcl10_chr3_145902271_R Primer3: not found at this Tm N.d. 0.43
mm2_exon_Clu_chr14_65974704_F TCGTCGGCAGCGTCTGGACAGGGAAGCTTAGGGA 59.8 0.39
mm2_exon_Clu_chr14_65974704_R GTCTCGTGGGCTCGGCGCTCTAGCCCTTCTGTTGG 60.4 0.39
mm4_intron_Mat2b_chr11_40692368_F TCGTCGGCAGCGTCGACTACTGAAACGGGAGCCA 59.3 0.36
mm4_intron_Mat2b_chr11_40692368_R GTCTCGTGGGCTCGGGTGGCGCCTTTGAGTTTGTT 59.8 0.36
mm4_intergenic_Uncx|Micall2_chr5_139613841_F TCGTCGGCAGCGTCCCATGTCTGTCTGGCACCTG 60.6 0.33
mm4_intergenic_Uncx|Micall2_chr5_139613841_R GTCTCGTGGGCTCGGCAAACCCCTCTTAGCAGGCA 59.9 0.33
mm4_intron_1700041M05Rik_chr4_125236423_F Primer3: not found at this Tm N.d. 0.33
mm4_intron_1700041M05Rik_chr4_125236423_R Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Gsta4|Gsta1_chr9_78228766_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Gsta4|Gsta1_chr9_78228766_R Primer3: not found at this Tm N.d. 0.31
mm4_intron_Arid3a_chr10_79932978_F Primer3: not found at this Tm N.d. 0.31
mm4_intron_Arid3a_chr10_79932978_R Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Rbfox3|Gm11750_chr11_118767281_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Rbfox3|Gm11750_chr11_118767281_R Primer3: not found at this Tm N.d. 0.29
mm4_intron_Tjp1_chr7_65370531_F TCGTCGGCAGCGTCGGTACCAACCACTCCTCCAG 59.3 0.27
mm4_intron_Tjp1_chr7_65370531_R GTCTCGTGGGCTCGGCCCCGGGTGAGGATTCTCA 60.6 0.27
mm4_intergenic_Rnf214|Rnf214/Pcsk7_chr9_45906297_F Primer3: not found at this Tm N.d. 0.26
mm4_intergenic_Rnf214|Rnf214/Pcsk7_chr9_45906297_R Primer3: not found at this Tm N.d. 0.26
mm4_intron_Cux2_chr5_122030644_F TCGTCGGCAGCGTCCTCCGTGAAGGCAGACAGC 60.7 0.26
mm4_intron_Cux2_chr5_122030644_R GTCTCGTGGGCTCGGTTGCATCTCCTCACTGCGAG 60.1 0.26
mm4_exon_Hck_chr2_153136741_F TCGTCGGCAGCGTCTTTCCCCAGCCACCTACAAC 59.8 0.25
mm4_exon_Hck_chr2_153136741_R GTCTCGTGGGCTCGGCATGCTGCAGCGACTTCATC 59.9 0.25
mm3_intron_Impdh1_chr6_29215976_F TCGTCGGCAGCGTCGCAATGGCAGTAGAGGGGAC 60.4 0.25
mm3_intron_Impdh1_chr6_29215976_R GTCTCGTGGGCTCGGTACGAGCCGGAGAGGTGAC 60.7 0.25
mm4_intergenic_Gm13076|Fam131c_chr4_141368095_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm13076|Fam131c_chr4_141368095_R Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Cxxc5|Gm23882_chr18_35868748_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Cxxc5|Gm23882_chr18_35868748_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Gm7784|B3gat2_chr1_23524619_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm7784|B3gat2_chr1_23524619_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Crbn|Gm22418_chr6_106800121_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Crbn|Gm22418_chr6_106800121_R Primer3: not found at this Tm N.d. 0.21
mm3_intergenic_Rpl30-ps9|Gm14824_chrX_105320445_F Primer3: not found at this Tm N.d. 0.20
mm3_intergenic_Rpl30-ps9|Gm14824_chrX_105320445_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm11468|Gm14268_chr2_166318552_F TCGTCGGCAGCGTCTCAGGTTCGGAAAGACCACG 59.9 0.19
mm4_intergenic_Gm11468|Gm14268_chr2_166318552_R GTCTCGTGGGCTCGGTGTGAGCCTGGAGGACTACA 59.8 0.19
mm4_intron_4932414N04Rik_chr2_68657752_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_4932414N04Rik_chr2_68657752_R Primer3: not found at this Tm N.d. 0.19
mm4_intron_Ahdc1_chr4_133016462_F TCGTCGGCAGCGTCGTCAGGCTTCCAAGAGCTGA 59.6 0.18
mm4_intron_Ahdc1_chr4_133016462_R GTCTCGTGGGCTCGGCTGGCTGGGCTCTTTCTCTC 60.1 0.18
mm4_exon_Slc16a2_chrX_103707915_F TCGTCGGCAGCGTCACTGAACCAGAAAGCGCTGT 60.1 0.18
mm4_exon_Slc16a2_chrX_103707915_R GTCTCGTGGGCTCGGTGTCCTTACGCTGCTCTCAC 59.7 0.18
mm4_intergenic_Fam46a|Gm25125_chr9_85469361_F TCGTCGGCAGCGTCCATCCCCTAGCACACAGAGG 59.5 0.18
mm4_intergenic_Fam46a|Gm25125_chr9_85469361_R GTCTCGTGGGCTCGGGGAGCGGCTTTTCTGTAGGA 59.7 0.18
mm4_intron_Snrnp35_chr5_124485317_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Snrnp35_chr5_124485317_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Rem2_chr14_54476545_F TCGTCGGCAGCGTCGGAGACCCCTGCACTTTTGA 59.8 0.17
mm4_intron_Rem2_chr14_54476545_R GTCTCGTGGGCTCGGTTAACCTAGGGGCCAAGTGC 59.6 0.17
mm4_intron_Zcchc11_chr4_108459714_F TCGTCGGCAGCGTCACCTCCATCCCCTCCTCATT 59.6 0.17
mm4_intron_Zcchc11_chr4_108459714_R GTCTCGTGGGCTCGGCCAAGCACCCGTCAAACCTC 61.5 0.17
mm4_intergenic_Lrrc55|Gm13713_chr2_85163729_F TCGTCGGCAGCGTCGGGATGGGCAATGTGGAGAA 60.0 0.16
mm4_intergenic_Lrrc55|Gm13713_chr2_85163729_R GTCTCGTGGGCTCGGCTGTCCCAGTAGCTAGGGGT 60.0 0.16
mm4_intron_Scaf8_chr17_3115619_F TCGTCGGCAGCGTCAGGGCTGAGGGAAGGCAG 61.0 0.16
mm4_intron_Scaf8_chr17_3115619_R GTCTCGTGGGCTCGGGGGTGGGAGGTGATAGTCCT 60.0 0.16
mm4_intergenic_Edil3|Gm26120_chr13_89364374_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Edil3|Gm26120_chr13_89364374_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Ccnb3_chrX_7029243_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Ccnb3_chrX_7029243_R Primer3: not found at this Tm N.d. 0.16
mm4_exon_Eml6_chr11_30025950_F Primer3: not found at this Tm N.d. 0.16
mm4_exon_Eml6_chr11_30025950_R Primer3: not found at this Tm N.d. 0.16
mm3_intergenic_4933402N22Rik|Sema3d_chr5_12240208_F Primer3: not found at this Tm N.d. 0.16
mm3_intergenic_4933402N22Rik|Sema3d_chr5_12240208_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm23534|Gm22727_chr1_137671266_F TCGTCGGCAGCGTCTGGATGGGTACGCATTGCTT 60.0 0.16
mm4_intergenic_Gm23534|Gm22727_chr1_137671266_R GTCTCGTGGGCTCGGTCCCCAGAGAACGAACTGAA 58.2 0.16
mm4_intergenic_Rpl36a-ps1|Mzt1_chr14_99012509_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Rpl36a-ps1|Mzt1_chr14_99012509_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm25180|Agap1_chr1_89580138_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm25180|Agap1_chr1_89580138_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Plekhm1_chr11_103408490_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Plekhm1_chr11_103408490_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_2700089E24Rik|Gm23403_chr6_133149131_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_2700089E24Rik|Gm23403_chr6_133149131_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Ubqln4_chr3_88554084_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Ubqln4_chr3_88554084_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Tmem59l|Crlf1_chr8_70487821_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Tmem59l|Crlf1_chr8_70487821_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gdap1|Gm25166_chr1_17504425_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gdap1|Gm25166_chr1_17504425_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm12123|Gm12122_chr11_35343548_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm12123|Gm12122_chr11_35343548_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm23221|Paqr5_chr9_62022186_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm23221|Paqr5_chr9_62022186_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gap|Zfy1_chrY_671347_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gap|Zfy1_chrY_671347_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Il34_chr8_110747165_F Primer3: not found at this Tm N.d. 0.14
mm4_intron_Il34_chr8_110747165_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_St6galnac5|St6galnac3_chr3_153064142_F TCGTCGGCAGCGTCCACCTGGACACTACAGCACG 60.6 0.13
mm4_intergenic_St6galnac5|St6galnac3_chr3_153064142_R GTCTCGTGGGCTCGGAGCAGGGGAAGACAGTCTGA 60.1 0.13
mm4_exon_Cecr6_chr6_120493461_F Primer3: not found at this Tm N.d. 0.13
mm4_exon_Cecr6_chr6_120493461_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Nub1_chr5_24686061_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Nub1_chr5_24686061_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Zmat4_chr8_23915657_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Zmat4_chr8_23915657_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm24121|Ugt2b35_chr5_86953788_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm24121|Ugt2b35_chr5_86953788_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm6317|Gm25383_chr13_78886693_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm6317|Gm25383_chr13_78886693_R Primer3: not found at this Tm N.d. 0.13
mm2_intergenic_Olfm1|Gm13373_chr2_28290288_F TCGTCGGCAGCGTCATGCACCTGCTTAATGCCCT 60.0 0.13
mm2_intergenic_Olfm1|Gm13373_chr2_28290288_R GTCTCGTGGGCTCGGAAAGACATGCAGGGCTCAGG 60.3 0.13
mm4_intergenic_Gm5083|Gm27007_chr13_44374757_F TCGTCGGCAGCGTCAGTGCACAACTCCAAGTGGG 60.4 0.13
mm4_intergenic_Gm5083|Gm27007_chr13_44374757_R GTCTCGTGGGCTCGGATGACTTCACAGGGTGGCAG 59.9 0.13
mm3_intron_Tspan4_chr7_141482178_F Primer3: not found at this Tm N.d. 0.13
mm3_intron_Tspan4_chr7_141482178_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Nav2_chr7_49196178_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Nav2_chr7_49196178_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Cnnm1_chr19_43440894_F TCGTCGGCAGCGTCGACGTGGAGGTCCTGGGG 61.3 0.12
mm4_exon_Cnnm1_chr19_43440894_R GTCTCGTGGGCTCGGCACCCGCACCTGGACCAG 62.7 0.12
mm4_intron_Eif4enif1_chr11_3227569_F TCGTCGGCAGCGTCTGGCAGGTAAGATGACTTCACA 59.3 0.12
mm4_intron_Eif4enif1_chr11_3227569_R GTCTCGTGGGCTCGGGAAAACTTCCCTTCCACAGCA 58.6 0.12
mm3_intergenic_Capn8|4922505E12Rik_chr1_182674839_F Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Capn8|4922505E12Rik_chr1_182674839_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Cyth1|Gm24060_chr11_118177370_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Cyth1|Gm24060_chr11_118177370_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Afap1l2_chr19_57006846_F TCGTCGGCAGCGTCCGACCTTGGAAGTCGGGATT 59.7 0.12
mm4_intron_Afap1l2_chr19_57006846_R GTCTCGTGGGCTCGGCTTGGTAGAAGAGAGGCGGC 60.1 0.12
mm4_intron_Tmod2_chr9_75601800_F TCGTCGGCAGCGTCTCAACACGCAACAGGATCCT 59.6 0.11
mm4_intron_Tmod2_chr9_75601800_R GTCTCGTGGGCTCGGTCGCTGTCACTCCCAGTTTC 59.9 0.11
mm4_intergenic_Bnip1|Nkx2-5_chr17_26828086_F TCGTCGGCAGCGTCGGGCTCAGAGGCTAATTGCT 59.8 0.11
mm4_intergenic_Bnip1|Nkx2-5_chr17_26828086_R GTCTCGTGGGCTCGGAGGCTGTGGGCTGAAATCAA 59.8 0.11
mm3_intron_Fblim1_chr4_141599204_F TCGTCGGCAGCGTCTCCTCAGGCTACCGGTCTTT 60.2 0.11
mm3_intron_Fblim1_chr4_141599204_R GTCTCGTGGGCTCGGCTGGGGCTGAACGACCAG 60.0 0.11
mm4_intron_Uggt2_chr14_119071436_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Uggt2_chr14_119071436_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Caln1_chr5_130618015_F TCGTCGGCAGCGTCTGAGACCTGCTTAGCACTGC 60.0 0.11
mm4_intron_Caln1_chr5_130618015_R GTCTCGTGGGCTCGGAGGGCTCTGAGTGTGAGACT 59.8 0.11
mm4_intron_Sez6_chr11_77960122_F TCGTCGGCAGCGTCCCAGCAGAACACGATCAGGT 60.0 0.11
mm4_intron_Sez6_chr11_77960122_R GTCTCGTGGGCTCGGCAGGTGGGTCTCTCAGAGTG 59.1 0.11
mm4_intron_Prpf18_chr2_4639984_F TCGTCGGCAGCGTCATGCTGAGCAGAAGAGCCAG 60.1 0.11
mm4_intron_Prpf18_chr2_4639984_R GTCTCGTGGGCTCGGGCTCCTTAGCTCCTTGGATTT 57.7 0.11
mm4_intron_Prpf18_chr2_4639949_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Prpf18_chr2_4639949_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Prpf18_chr2_4639914_F Primer3: not found at this Tm N.d. 0.11
mm4_intron_Prpf18_chr2_4639914_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Oacyl|Sec11c_chr18_65781998_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Oacyl|Sec11c_chr18_65781998_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Erg|Ets2_chr16_95617123_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Erg|Ets2_chr16_95617123_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Ctdsp2_chr10_126979703_F TCGTCGGCAGCGTCCCGGAGTTTTCACGCAGGA 60.3 0.10
mm4_intron_Ctdsp2_chr10_126979703_R GTCTCGTGGGCTCGGGTGAACTTCGTCCCGAGTCC 60.3 0.10
mm4_intergenic_Wnt4|Cdc42_chr4_137315009_F TCGTCGGCAGCGTCTTCAGTCATTACACGCCCCG 60.3 0.10
mm4_intergenic_Wnt4|Cdc42_chr4_137315009_R GTCTCGTGGGCTCGGAGTTAGCAAGGCCATGTTGGT 60.2 0.10
mm4_intergenic_Mgat4a|2010300C02Rik_chr1_37603328_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Mgat4a|2010300C02Rik_chr1_37603328_R Primer3: not found at this Tm N.d. 0.10
mm4_exon_Cadm1_chr9_47530200_F Primer3: not found at this Tm N.d. 0.09
mm4_exon_Cadm1_chr9_47530200_R Primer3: not found at this Tm N.d. 0.09
mm4_exon_Usp49/Gm20517_chr17_47672699_F TCGTCGGCAGCGTCCTGGAGGAGCTGGCCAGT 61.0 0.09
mm4_exon_Usp49/Gm20517_chr17_47672699_R GTCTCGTGGGCTCGGGAGGTAGCGAGGGTGGCA 61.0 0.09
mm4_exon_Ephb4_chr5_137372201_F TCGTCGGCAGCGTCGTCAGCGCTCTGGACAAGAT 60.1 0.09
mm4_exon_Ephb4_chr5_137372201_R GTCTCGTGGGCTCGGGGACTCTTGGGTTGTGGGTC 60.2 0.09
mm4_intron_B230219D22Rik_chr13_55693576_F TCGTCGGCAGCGTCAGTCTCGCCCTGGAGCTG 61.0 0.09
mm4_intron_B230219D22Rik_chr13_55693576_R GTCTCGTGGGCTCGGTCTTCTCTGCGCTCCTTAGC 59.5 0.09
mm3_intron_Stk10_chr11_32604331_F Primer3: not found at this Tm N.d. 0.09
mm3_intron_Stk10_chr11_32604331_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm22649|Usp29_chr7_6953311_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm22649|Usp29_chr7_6953311_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Taf1b|Grhl1_chr12_24566625_F TCGTCGGCAGCGTCGTTGCTCACAGGGGACTCAA 59.8 0.09
mm4_intergenic_Taf1b|Grhl1_chr12_24566625_R GTCTCGTGGGCTCGGGCCAGCCAGAGATGAAGGAC 60.4 0.09
mm4_exon_B4galt7_chr13_55600044_F Primer3: not found at this Tm N.d. 0.09
mm4_exon_B4galt7_chr13_55600044_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Lrrc41_chr4_116075661_F TCGTCGGCAGCGTCGGGGTAAGTCGCGGAGTG 59.8 0.09
mm4_intron_Lrrc41_chr4_116075661_R GTCTCGTGGGCTCGGGACTCCACCATCGAACTCCC 59.8 0.09
mm4_intron_Atg5_chr10_44268681_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Atg5_chr10_44268681_R Primer3: not found at this Tm N.d. 0.09
mm3_intron_Poteg_chr8_27475538_F Primer3: not found at this Tm N.d. 0.09
mm3_intron_Poteg_chr8_27475538_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Ddx18|Gm15394_chr1_121766280_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Ddx18|Gm15394_chr1_121766280_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Fosl2_chr5_32152046_F TCGTCGGCAGCGTCACAAGTCAGGTGCTGTCTGT 59.1 0.08
mm4_intron_Fosl2_chr5_32152046_R GTCTCGTGGGCTCGGTTACCATCTCAGCCCACCAG 59.0 0.08
mm4_intron_Ndrg2_chr14_51911843_F TCGTCGGCAGCGTCCCAAGACACACGGAGAGCTT 59.9 0.08
mm4_intron_Ndrg2_chr14_51911843_R GTCTCGTGGGCTCGGCGAGGTGAGAATGTTGGGGT 59.6 0.08
mm4_intergenic_Gm24341|Cnr1_chr4_33713648_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm24341|Cnr1_chr4_33713648_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Rab27b|Dynap_chr18_70172438_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Rab27b|Dynap_chr18_70172438_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Igf2bp3_chr6_49117016_F TCGTCGGCAGCGTCTGTGAGACCAACAACGCATG 59.0 0.08
mm4_intron_Igf2bp3_chr6_49117016_R GTCTCGTGGGCTCGGTCAGGGTTGGATGCAGGAAC 59.9 0.08
mm4_intron_Glipr2_chr4_43972426_F TCGTCGGCAGCGTCCCTTAGGTGCAGATCTGGGAC 59.8 0.08
mm4_intron_Glipr2_chr4_43972426_R GTCTCGTGGGCTCGGTTGCCATCTGAAGCTCAGGC 60.6 0.08
mm4_intergenic_Gm15219|Cdkn3_chr14_46579158_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm15219|Cdkn3_chr14_46579158_R Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Gm15998|Efna3_chr3_89320929_F TCGTCGGCAGCGTCTCTCACGATTTATGGGGCCG 59.8 0.08
mm3_intergenic_Gm15998|Efna3_chr3_89320929_R GTCTCGTGGGCTCGGACCGACGGAGGTAGGAGG 59.7 0.08
mm4_intron_Pard3_chr8_127274990_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Pard3_chr8_127274990_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Tmem82_chr4_141618102_F TCGTCGGCAGCGTCGGGAGGGACTAGGAAAGGGT 59.9 0.08
mm4_exon_Tmem82_chr4_141618102_R GTCTCGTGGGCTCGGAGTGCCACTGAATCCAGCAA 59.8 0.08
mm4_exon_Anpep_chr7_79842096_F TCGTCGGCAGCGTCCATCTACAGCAGGGGTGGTG 60.1 0.08
mm4_exon_Anpep_chr7_79842096_R GTCTCGTGGGCTCGGGGCAGCTGTGTGTACCATCA 60.3 0.08
mm4_intergenic_Gm24338|Ppp2r3a_chr9_101251415_F TCGTCGGCAGCGTCTCCTGTTAACGAGTTCCCGG 59.3 0.08
mm4_intergenic_Gm24338|Ppp2r3a_chr9_101251415_R GTCTCGTGGGCTCGGCCACGCCAGAGGACTCCC 61.7 0.08
mm4_intergenic_Hdac5/mmu-mir-8101|BC030867_chr11_102248443_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Hdac5/mmu-mir-8101|BC030867_chr11_102248443_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm21614|Gm23249_chr12_93648223_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm21614|Gm23249_chr12_93648223_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm12580|Olfr267_chr4_58666345_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm12580|Olfr267_chr4_58666345_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Ccne1|Gm22203_chr7_38128470_F TCGTCGGCAGCGTCTAGTCATAGGCCTGGGAGCT 59.4 0.08
mm4_intergenic_Ccne1|Gm22203_chr7_38128470_R GTCTCGTGGGCTCGGCTGGCCTCTACACACTCACG 60.1 0.08
mm4_exon_Gm4980/Gm10605_chr7_99626955_F Primer3: not found at this Tm N.d. 0.07
mm4_exon_Gm4980/Gm10605_chr7_99626955_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm26859|Syt7_chr19_10402886_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm26859|Syt7_chr19_10402886_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_AC124326.1|Mir802_chr16_93087459_F TCGTCGGCAGCGTCAGGCTACAATGTGGCGAGAG 59.8 0.07
mm4_intergenic_AC124326.1|Mir802_chr16_93087459_R GTCTCGTGGGCTCGGTGTCCCAGTCTCCCTGTCTT 59.8 0.07
mm4_intergenic_Gm5356|Gm26331_chr8_89242210_F TCGTCGGCAGCGTCGCAGTGGCTTTTGAACCGAG 60.0 0.06
mm4_intergenic_Gm5356|Gm26331_chr8_89242210_R GTCTCGTGGGCTCGGGGTGTTCCCTGGCTAACTGG 60.3 0.06
mm4_intergenic_Gm12125|Tenm2_chr11_36002603_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm12125|Tenm2_chr11_36002603_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Nap1l4_chr7_143545155_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Nap1l4_chr7_143545155_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Phf1_chr17_26933422_F TCGTCGGCAGCGTCCTTTGGCTGCTGCGTCATAC 59.9 0.06
mm4_exon_Phf1_chr17_26933422_R GTCTCGTGGGCTCGGGGGAAGACTGAGTGTGAGCG 60.3 0.06
mm4_intergenic_Fnbp1l|Pde5a_chr3_122727713_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Fnbp1l|Pde5a_chr3_122727713_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Metap1|Eif4e_chr3_138489657_F TCGTCGGCAGCGTCCTGACAGCCACGCCCTCTC 62.6 0.06
mm4_intergenic_Metap1|Eif4e_chr3_138489657_R GTCTCGTGGGCTCGGGTGTAGGATCTCCGCTGACC 59.6 0.06
mm4_intron_Eif2ak3_chr6_70867917_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Eif2ak3_chr6_70867917_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Catsperb|Tc2n/Gm16285_chr12_101630320_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Catsperb|Tc2n/Gm16285_chr12_101630320_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_A830012C17Rik|Gm11780_chr4_4252358_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_A830012C17Rik|Gm11780_chr4_4252358_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Tnfrsf21|Gpr110_chr17_43264081_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Tnfrsf21|Gpr110_chr17_43264081_R Primer3: not found at this Tm N.d. 0.06
mm4_exon_Drap1_chr19_5424797_F TCGTCGGCAGCGTCTTCTTGCTTGGCATCTCGGG 60.6 0.06
mm4_exon_Drap1_chr19_5424797_R GTCTCGTGGGCTCGGGGAGTAAACGGCACCAGGAC 60.6 0.06
mm4_intron_Zcchc6_chr13_59780398_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Zcchc6_chr13_59780398_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Eya4|Rps12_chr10_23387942_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Eya4|Rps12_chr10_23387942_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Ccdc33_chr9_58113454_F TCGTCGGCAGCGTCCCGTAGCAGGTGGAAGCTTT 60.3 0.06
mm4_intron_Ccdc33_chr9_58113454_R GTCTCGTGGGCTCGGCTGCCCATTCACTCTCAGGG 60.1 0.06
mm4_intron_Clip1_chr5_123684060_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Clip1_chr5_123684060_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm23624|4930593C16Rik_chr9_120846292_F TCGTCGGCAGCGTCATCCCAGATAGCCCAAGGGT 60.0 0.06
mm4_intergenic_Gm23624|4930593C16Rik_chr9_120846292_R GTCTCGTGGGCTCGGTGGCCACAGTCCACACTATG 59.6 0.06
mm4_intergenic_Gm14236|Gm24327_chr2_168034059_F TCGTCGGCAGCGTCGCTCCCTTTCGCTGTCCTT 60.0 0.06
mm4_intergenic_Gm14236|Gm24327_chr2_168034059_R GTCTCGTGGGCTCGGAGCTTTCTGTAGGCTGCTGG 60.0 0.06
mm3_intron_Gm8091_chr5_100272309_F Primer3: not found at this Tm N.d. 0.05
mm3_intron_Gm8091_chr5_100272309_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Rragc|Gm12916_chr4_124291608_F TCGTCGGCAGCGTCCCCCGTTGTCCTTGAGTGAG 60.3 0.05
mm3_intergenic_Rragc|Gm12916_chr4_124291608_R GTCTCGTGGGCTCGGGGAGGTGGGGATGCAAATGA 60.0 0.05
mm4_intron_Wdfy3_chr5_101867415_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Wdfy3_chr5_101867415_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Ptplad2|Ifnb1_chr4_88447868_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Ptplad2|Ifnb1_chr4_88447868_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Tnfaip8_chr18_49997644_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Tnfaip8_chr18_49997644_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Thsd7a|Gm16123_chr6_12563047_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Thsd7a|Gm16123_chr6_12563047_R Primer3: not found at this Tm N.d. 0.05
mm4_exon_Mkl1_chr15_81026467_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Mkl1_chr15_81026467_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Dhrs3|Vps13d_chr4_144938916_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Dhrs3|Vps13d_chr4_144938916_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Tjp2|Fxn_chr19_24225517_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Tjp2|Fxn_chr19_24225517_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Dcst1|Zbtb7b_chr3_89365636_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Dcst1|Zbtb7b_chr3_89365636_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Chd6_chr2_161017889_F TCGTCGGCAGCGTCCCTCGCAGTTCCAGGTTACA 59.6 0.05
mm4_intron_Chd6_chr2_161017889_R GTCTCGTGGGCTCGGAGTATCCCATGGCCTTGTGC 60.1 0.05
mm4_intergenic_Kcnc4|Slc6a17_chr3_107459057_F TCGTCGGCAGCGTCCTTCTGCGGAGTGGCTCTG 60.4 0.05
mm4_intergenic_Kcnc4|Slc6a17_chr3_107459057_R GTCTCGTGGGCTCGGAAGCCAACTCTTCCTGCTCC 59.9 0.05
mm4_intergenic_Gm23186|Arid1b_chr17_4763300_F TCGTCGGCAGCGTCAACAGGCAGAAGCAACCTGT 60.1 0.05
mm4_intergenic_Gm23186|Arid1b_chr17_4763300_R GTCTCGTGGGCTCGGGAAAGAGGCCTGGTTCCACA 59.8 0.05
mm3_intron_Sez6_chr11_77931461_F TCGTCGGCAGCGTCTCCACTCCAGGCGTCTAGG 60.3 0.05
mm3_intron_Sez6_chr11_77931461_R GTCTCGTGGGCTCGGTCGAGCGAAGTTTGGAGACC 60.0 0.05
mm4_intergenic_Gm26626|Runx1_chr16_92634574_F TCGTCGGCAGCGTCTGCTCACCCATATGCATCCC 59.8 0.05
mm4_intergenic_Gm26626|Runx1_chr16_92634574_R GTCTCGTGGGCTCGGGGTGGGCCACTCCTTCTTTT 60.1 0.05
mm4_intron_Dach2_chrX_113726532_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Dach2_chrX_113726532_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Spty2d1|Tmem86a_chr7_47008600_F TCGTCGGCAGCGTCGCGAGAGAGGGTTTCAGTGT 59.6 0.05
mm4_intergenic_Spty2d1|Tmem86a_chr7_47008600_R GTCTCGTGGGCTCGGTCTGTACATCCACGCGTGAC 60.1 0.05
mm4_intergenic_Gm5616|Rexo2_chr9_48468338_F TCGTCGGCAGCGTCACGTGGTGGAGAGTGGTCTA 59.8 0.05
mm4_intergenic_Gm5616|Rexo2_chr9_48468338_R GTCTCGTGGGCTCGGCGTGTGTTTCGAACAGGGAG 59.1 0.05
mm4_intergenic_Elk3|mmu-mir-7688_chr10_93376680_F TCGTCGGCAGCGTCTCGGAGTCAACATTCCCCAA 58.9 0.05
mm4_intergenic_Elk3|mmu-mir-7688_chr10_93376680_R GTCTCGTGGGCTCGGTCTCTTGTCCTGAGTTGCCT 58.2 0.05
mm4_intergenic_Smug1|Cbx5_chr15_103177359_F TCGTCGGCAGCGTCAGGCTCGTCCCATCCAATTG 60.1 0.04
mm4_intergenic_Smug1|Cbx5_chr15_103177359_R GTCTCGTGGGCTCGGTCTGTAGAGGGATCAGGGCC 60.1 0.04
mm4_intron_Eps8_chr6_137482763_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Eps8_chr6_137482763_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Gm26801_chr1_177442275_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Gm26801_chr1_177442275_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Tiam1_chr16_89832645_F TCGTCGGCAGCGTCACCGAGGAAAAGGAGCAAGG 59.9 0.04
mm4_intron_Tiam1_chr16_89832645_R GTCTCGTGGGCTCGGGATCGCATCAGATCCTGGCA 59.9 0.04
mm4_intron_Anxa3_chr5_96796831_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Anxa3_chr5_96796831_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Frmd4a_chr2_4601199_F TCGTCGGCAGCGTCTTACAGTCGCCACACAAGGG 60.2 0.04
mm4_exon_Frmd4a_chr2_4601199_R GTCTCGTGGGCTCGGGTGATAGTGCAGCTGCCTGA 60.1 0.04
mm4_intron_Trio_chr15_27834999_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Trio_chr15_27834999_R Primer3: not found at this Tm N.d. 0.04
mm3_intron_Zzz3_chr3_152396151_F Primer3: not found at this Tm N.d. 0.04
mm3_intron_Zzz3_chr3_152396151_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Dlg1_chr16_31833904_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Dlg1_chr16_31833904_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm10505|Grm4_chr17_27418492_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm10505|Grm4_chr17_27418492_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Wwox|Gm16116_chr8_114568478_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Wwox|Gm16116_chr8_114568478_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Thbs4|Mtx3_chr13_92794858_F TCGTCGGCAGCGTCCCCGCGCTTTATGGTCCA 60.1 0.04
mm4_intergenic_Thbs4|Mtx3_chr13_92794858_R GTCTCGTGGGCTCGGGCTCTTCCTGGCAAATGGGG 61.3 0.04
mm4_intergenic_Gm10220|Gm7361_chr5_26234307_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm10220|Gm7361_chr5_26234307_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_4930445N18Rik|Zfp516_chr18_82873952_F TCGTCGGCAGCGTCGAGCCAACAGAGCTGACACT 59.9 0.04
mm4_intergenic_4930445N18Rik|Zfp516_chr18_82873952_R GTCTCGTGGGCTCGGTTACCTCCACAGGAATGCCC 59.3 0.04
mm3_intergenic_Morc1|Trat1_chr16_48710839_F Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Morc1|Trat1_chr16_48710839_R Primer3: not found at this Tm N.d. 0.04
mm3_intergenic_Prima1|Fam181a_chr12_103242696_F TCGTCGGCAGCGTCGGAAAACAGACGTGAGCGGA 60.5 0.04
mm3_intergenic_Prima1|Fam181a_chr12_103242696_R GTCTCGTGGGCTCGGAGACGAGGAATCACTGCAGC 60.1 0.04
mm4_intron_Sos1_chr17_80469097_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Sos1_chr17_80469097_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ogfrl1|Gm7784_chr1_23383331_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ogfrl1|Gm7784_chr1_23383331_R Primer3: not found at this Tm N.d. 0.04
mm4_exon_Myo16_chr8_10634659_F TCGTCGGCAGCGTCTGTCTTTCTGCCCACTGTCC 59.8 0.04
mm4_exon_Myo16_chr8_10634659_R GTCTCGTGGGCTCGGGGCAATGGCGGAAATGAATGA 59.8 0.04
mm4_intergenic_Gm23752|Gm24815_chr12_7171843_F TCGTCGGCAGCGTCACGGAATGCTCCAGTCCTTG 60.0 0.03
mm4_intergenic_Gm23752|Gm24815_chr12_7171843_R GTCTCGTGGGCTCGGTGGGAGATGGTGAGAGACAGA 59.6 0.03
mm4_intron_Spata21_chr4_141097164_F TCGTCGGCAGCGTCCCAACCCTGTCCAGCTCTTC 60.3 0.03
mm4_intron_Spata21_chr4_141097164_R GTCTCGTGGGCTCGGCCGCTCCTAATCTCTGCACA 59.5 0.03
mm4_intron_Dock9_chr14_121727260_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Dock9_chr14_121727260_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm10782|Slc25a48_chr13_56432662_F TCGTCGGCAGCGTCGCTCCAGCCCATCAGTATCC 59.9 0.03
mm4_intergenic_Gm10782|Slc25a48_chr13_56432662_R GTCTCGTGGGCTCGGTAGCAAGTGGGAAGATGGCG 60.1 0.03
mm4_intergenic_Crispld2|Gm15684_chr8_120041715_F TCGTCGGCAGCGTCGCGCTATGTGATGGGCCTAG 60.6 0.03
mm4_intergenic_Crispld2|Gm15684_chr8_120041715_R GTCTCGTGGGCTCGGGCCGCTTTGCTGTAGCTTTT 60.0 0.03
mm4_intergenic_Cacng2|Cacng2/A730060N03Rik_chr15_78075639_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cacng2|Cacng2/A730060N03Rik_chr15_78075639_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Polm_chr11_5835585_F Primer3: not found at this Tm N.d. 0.03
mm4_exon_Polm_chr11_5835585_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Nedd4_chr9_72662662_F TCGTCGGCAGCGTCGTTCATCGTGGCGAGGGG 60.5 0.03
mm4_intron_Nedd4_chr9_72662662_R GTCTCGTGGGCTCGGCGCCTCTCTCCATCTCCCC 61.2 0.03
mm3_intron_Hoxc9_chr15_102977535_F Primer3: not found at this Tm N.d. 0.03
mm3_intron_Hoxc9_chr15_102977535_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Wasf1_chr10_40884207_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Wasf1_chr10_40884207_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Osbpl8_chr10_111165438_F TCGTCGGCAGCGTCAGCGGTACAAAGCCCAGTG 60.3 0.03
mm4_intron_Osbpl8_chr10_111165438_R GTCTCGTGGGCTCGGCTGTCAAACTTCGGGAGGGG 60.3 0.03
mm4_intergenic_Gm11650|Cacng4_chr11_107794160_F TCGTCGGCAGCGTCTTCTTTGTGTCTCGCGTGGA 59.8 0.03
mm4_intergenic_Gm11650|Cacng4_chr11_107794160_R GTCTCGTGGGCTCGGTTGCATCGAAGGTAGGACCC 59.4 0.03
mm4_intergenic_Adamts6|Cwc27_chr13_104510294_F TCGTCGGCAGCGTCGAGGGACAGATGTGGCTTCC 60.1 0.03
mm4_intergenic_Adamts6|Cwc27_chr13_104510294_R GTCTCGTGGGCTCGGCCAGTGTCCAGATGGCAGAG 60.1 0.03
mm4_intron_Fras1_chr5_96617427_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Fras1_chr5_96617427_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cela2a|Ctrc_chr4_141836457_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cela2a|Ctrc_chr4_141836457_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Tacc3|Fgfr3_chr5_33689460_F TCGTCGGCAGCGTCTGGGCAAATTCAGAAGGGCT 59.8 0.03
mm4_intergenic_Tacc3|Fgfr3_chr5_33689460_R GTCTCGTGGGCTCGGGAGTCCCTTGAGTCCAGCC 59.4 0.03
mm4_intron_Mpped1_chr15_83846661_F TCGTCGGCAGCGTCACACCAGGATCCAGGTTCCT 60.1 0.03
mm4_intron_Mpped1_chr15_83846661_R GTCTCGTGGGCTCGGGCCCCTGGCTAATCATCCTC 59.9 0.03
mm4_exon_Anks1b_chr10_90829454_F TCGTCGGCAGCGTCGAGCTCGTGGGGATCCGG 61.9 0.03
mm4_exon_Anks1b_chr10_90829454_R GTCTCGTGGGCTCGGCCTTAGCCGTCGCCTCTAAG 59.9 0.03
mm4_intron_Afg3l1_chr8_123478077_F TCGTCGGCAGCGTCCTGTGGCATTGCTCTGCAG 59.4 0.02
mm4_intron_Afg3l1_chr8_123478077_R GTCTCGTGGGCTCGGAGGCAGAAATCGGGAAGACG 60.1 0.02
mm4_intron_Siglece_chr7_43658050_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Siglece_chr7_43658050_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Dgkd_chr1_87853291_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Dgkd_chr1_87853291_R Primer3: not found at this Tm N.d. 0.02
mm2_intergenic_Gm22227|Zfp703_chr8_26595343_F Primer3: not found at this Tm N.d. 0.02
mm2_intergenic_Gm22227|Zfp703_chr8_26595343_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Pcolce_chr5_137610682_F TCGTCGGCAGCGTCCAGTCCAAGGACCACGGAAA 59.8 0.02
mm4_exon_Pcolce_chr5_137610682_R GTCTCGTGGGCTCGGGGGGTAGAGGAGGTTAGGGG 60.1 0.02
mm4_intron_Slitrk2_chrX_66652911_F TCGTCGGCAGCGTCCACTCACAGGGAAGGGAAGC 60.3 0.02
mm4_intron_Slitrk2_chrX_66652911_R GTCTCGTGGGCTCGGAGAGGTTTTGCAGGCTCCTC 59.9 0.02
mm4_intron_Elf2_chr3_51276961_F TCGTCGGCAGCGTCCGACTCCCCAACTTAGCCG 60.1 0.02
mm4_intron_Elf2_chr3_51276961_R GTCTCGTGGGCTCGGGAGTAACCTGCCTCGGATCC 59.6 0.02
mm3_exon_Snx27_chr3_94582441_F TCGTCGGCAGCGTCATTCGGACTTGACAATGCGC 59.5 0.02
mm3_exon_Snx27_chr3_94582441_R GTCTCGTGGGCTCGGACGAGGACGGGGAAGGTATT 60.3 0.02
mm4_intergenic_Gm5544|Notch2_chr3_97977990_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm5544|Notch2_chr3_97977990_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Bri3|Nptx2_chr5_144510698_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Bri3|Nptx2_chr5_144510698_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gpr108|Trip10_chr17_57248622_F TCGTCGGCAGCGTCGGCGGAGAAAGCACCTACAG 60.7 0.02
mm4_intergenic_Gpr108|Trip10_chr17_57248622_R GTCTCGTGGGCTCGGCTGGGGCTTAGGATTTCGGG 60.1 0.02
mm3_intron_Nkx6-3_chr8_23154502_F Primer3: not found at this Tm N.d. 0.02
mm3_intron_Nkx6-3_chr8_23154502_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Asb3|Gm12105_chr11_31111519_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Asb3|Gm12105_chr11_31111519_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ddx11|Soga2_chr17_66290106_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Ddx11|Soga2_chr17_66290106_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Krt2|Krt1_chr15_101818615_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Krt2|Krt1_chr15_101818615_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_A530064D06Rik|Trem1_chr17_48188687_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_A530064D06Rik|Trem1_chr17_48188687_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Gm11100_chr14_13937637_F TCGTCGGCAGCGTCGTGGACAGATCCCAGTGTGG 60.0 0.02
mm4_intron_Gm11100_chr14_13937637_R GTCTCGTGGGCTCGGAACAAGAGAGAACAGCGGGA 58.9 0.02
mm4_intron_Cabin1_chr10_75706140_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Cabin1_chr10_75706140_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Mrpl48_chr7_100572247_F TCGTCGGCAGCGTCCAGGCTTCAGCTCCAGTTCA 59.9 0.02
mm4_intron_Mrpl48_chr7_100572247_R GTCTCGTGGGCTCGGGGTGTGTCAGAGCAGTCCAT 59.6 0.02
mm3_intron_Mgat5_chr1_127304728_F Primer3: not found at this Tm N.d. 0.02
mm3_intron_Mgat5_chr1_127304728_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Nrg2_chr18_36196030_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Nrg2_chr18_36196030_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Dgki_chr6_37187530_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Dgki_chr6_37187530_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Bmp6_chr13_38394704_F TCGTCGGCAGCGTCGGTAGAGTCCAGGTGCCATG 59.8 0.01
mm4_intron_Bmp6_chr13_38394704_R GTCTCGTGGGCTCGGAACCCACCACACTAGCTTGG 59.8 0.01
mm4_intergenic_Gm22684|Anxa1_chr19_20275103_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22684|Anxa1_chr19_20275103_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm15131|Maged2_chrX_150768182_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm15131|Maged2_chrX_150768182_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_N6amt2_chr14_57571508_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_N6amt2_chr14_57571508_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tmem206_chr1_191328604_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Tmem206_chr1_191328604_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22097|Gm23041_chr10_109101233_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22097|Gm23041_chr10_109101233_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Jarid2_chr13_44730343_F TCGTCGGCAGCGTCCTCCAACCCCTCGCTGAC 59.7 0.01
mm3_intron_Jarid2_chr13_44730343_R GTCTCGTGGGCTCGGCGCCTGTGGAATCGAGGAAT 60.1 0.01
mm4_exon_Diras1_chr10_81019769_F TCGTCGGCAGCGTCGTCCCCAGCACCAGAGATG 59.7 0.01
mm4_exon_Diras1_chr10_81019769_R GTCTCGTGGGCTCGGCTTCTCCTGGCTGGCCTTG 60.3 0.01
mm4_intron_Sirt1_chr10_63339100_F TCGTCGGCAGCGTCCTCGGCTGCGGGAGATTTAA 60.1 0.01
mm4_intron_Sirt1_chr10_63339100_R GTCTCGTGGGCTCGGGTGACCCGGCGTGTTGTG 61.6 0.01
mm4_intron_Hic1_chr11_75168680_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Hic1_chr11_75168680_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Kcnj8|Abcc9_chr6_142571503_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Kcnj8|Abcc9_chr6_142571503_R Primer3: not found at this Tm N.d. 0.01
mm3_exon_Gm10760/Mctp1_chr13_76384445_F Primer3: not found at this Tm N.d. 0.01
mm3_exon_Gm10760/Mctp1_chr13_76384445_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Nrg3|Gm10713_chr14_39473278_F TCGTCGGCAGCGTCTGTGTGCGCTCCAGCCTC 62.6 0.01
mm4_intergenic_Nrg3|Gm10713_chr14_39473278_R GTCTCGTGGGCTCGGACGTGAGGATTTGAGGTGGG 59.6 0.01
mm4_intron_Nrg2_chr18_36037082_F TCGTCGGCAGCGTCGCCAAGGCAGTGATAAGGGT 60.0 0.01
mm4_intron_Nrg2_chr18_36037082_R GTCTCGTGGGCTCGGGGTCTTGACCAGGCTTTCCT 59.5 0.01
mm4_intergenic_Gm12142|A230004M16Rik_chr11_41605430_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm12142|A230004M16Rik_chr11_41605430_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxl4|Pou3f2_chr4_22477760_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Fbxl4|Pou3f2_chr4_22477760_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Atg5|Prdm1_chr10_44368925_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Atg5|Prdm1_chr10_44368925_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_A430105J06Rik|Gm3809_chr1_186795034_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_A430105J06Rik|Gm3809_chr1_186795034_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Klf10_chr15_38299855_F TCGTCGGCAGCGTCTCTGCAGGCTCCCAGACG 61.3 0.01
mm4_intron_Klf10_chr15_38299855_R GTCTCGTGGGCTCGGCAGGGTCCTGCCTTGGAG 59.3 0.01
mm3_intergenic_Asphd2|Sez6l_chr5_112395407_F TCGTCGGCAGCGTCTCCTTGGAACAGTGCATGGG 60.2 0.01
mm3_intergenic_Asphd2|Sez6l_chr5_112395407_R GTCTCGTGGGCTCGGGAGGACAGACACAGATGGGC 60.1 0.01
mm4_intergenic_Ralyl|Gm25981_chr3_13818202_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Ralyl|Gm25981_chr3_13818202_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_G730013B05Rik_chr16_50530245_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_G730013B05Rik_chr16_50530245_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22495|Dtna_chr18_23310015_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22495|Dtna_chr18_23310015_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Arl4c_chr1_88702220_F TCGTCGGCAGCGTCTCTAGTCCCGCCCACAGG 60.3 0.01
mm4_exon_Arl4c_chr1_88702220_R GTCTCGTGGGCTCGGTGGGCGAGTTTCGAACGATC 60.7 0.01
mm4_intron_Lmo3_chr6_138551012_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Lmo3_chr6_138551012_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Crtac1_chr19_42310505_F TCGTCGGCAGCGTCCCTCACCTGCTTCCTGGAAA 59.5 0.01
mm4_intron_Crtac1_chr19_42310505_R GTCTCGTGGGCTCGGACTCCAGCAGCTTGTGAGAA 59.2 0.01
mm4_intergenic_AY702102|A630075F10Rik_chr2_169997009_F TCGTCGGCAGCGTCTCATTACTGGAGGCGAGGGA 60.0 0.01
mm4_intergenic_AY702102|A630075F10Rik_chr2_169997009_R GTCTCGTGGGCTCGGTCCTCTCTCTTCCCAGAGCC 60.0 0.01
mm4_intergenic_Slain1|Ednrb_chr14_103755222_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Slain1|Ednrb_chr14_103755222_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Coro1c_chr5_113849647_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Coro1c_chr5_113849647_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Phlpp2_chr8_109868877_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Phlpp2_chr8_109868877_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Cacnb4_chr2_52676381_F TCGTCGGCAGCGTCCGTACGAGGACGACATGGTT 59.8 0.01
mm4_exon_Cacnb4_chr2_52676381_R GTCTCGTGGGCTCGGAGGAGCCGGGTAGGGAAG 60.0 0.01
mm4_intergenic_Sh3pxd2a|Obfc1_chr19_47465164_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Sh3pxd2a|Obfc1_chr19_47465164_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_Adra2c_chr5_35279251_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_Adra2c_chr5_35279251_R Primer3: not found at this Tm N.d. 0.01
mm4_exon_C77080_chr4_129248342_F Primer3: not found at this Tm N.d. 0.01
mm4_exon_C77080_chr4_129248342_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Exoc4_chr6_33249349_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Exoc4_chr6_33249349_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm14169|Dlgap4_chr2_156613491_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm14169|Dlgap4_chr2_156613491_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Pard3b|Gm11610_chr1_62441938_F TCGTCGGCAGCGTCGGCCAGGCATTTCAACACTC 59.7 0.01
mm4_intergenic_Pard3b|Gm11610_chr1_62441938_R GTCTCGTGGGCTCGGCCAGTACTCCAGGGTTGGGA 60.5 0.01
mm4_exon_Inpp5a_chr7_139389380_F TCGTCGGCAGCGTCCCACCCAGTCCAGAGCCC 61.7 0.01
mm4_exon_Inpp5a_chr7_139389380_R GTCTCGTGGGCTCGGGGCACTTACGTCGTCGAAGA 60.1 0.01
mm4_intergenic_Ccne1|Gm22203_chr7_38130150_F TCGTCGGCAGCGTCGGCCTCTTCAACAGACTGCT 59.9 0.01
mm4_intergenic_Ccne1|Gm22203_chr7_38130150_R GTCTCGTGGGCTCGGGCTCGATCCTGCTCTCCAC 59.9 0.01
mm4_exon_Cited4_chr4_120667161_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cited4_chr4_120667161_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cryl1|Gm23593_chr14_57320717_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cryl1|Gm23593_chr14_57320717_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Has1_chr17_17850049_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Has1_chr17_17850049_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cenpb_chr2_131179934_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Cenpb_chr2_131179934_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3glb1_chr3_144719804_F TCGTCGGCAGCGTCGGTGAACCCAAGGTGTCCG 60.6 0.00
mm4_intron_Sh3glb1_chr3_144719804_R GTCTCGTGGGCTCGGTACCCAGGCCTGCCGATG 61.4 0.00
mm4_exon_Pianp_chr6_124996773_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Pianp_chr6_124996773_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Adamtsl3_chr7_82396989_F TCGTCGGCAGCGTCTCCTGTGAAGAGGACTGGGT 59.8 0.00
mm4_intron_Adamtsl3_chr7_82396989_R GTCTCGTGGGCTCGGCTGGCCTCTCTTGTGTCCTG 60.0 0.00
mm4_exon_Plod2_chr9_92542467_F TCGTCGGCAGCGTCCGTCTCGCGAGAAGTCCTC 59.9 0.00
mm4_exon_Plod2_chr9_92542467_R GTCTCGTGGGCTCGGGAGAGCAGGGCGAGCATG 60.5 0.00
mm4_exon_Tfap2c_chr2_172549709_F TCGTCGGCAGCGTCCACATCTCTGTGCACAGCCA 60.6 0.00
mm4_exon_Tfap2c_chr2_172549709_R GTCTCGTGGGCTCGGCCCGAGCGGTAAATCCACAA 60.3 0.00
mm4_intergenic_Gm24298|4921513I03Rik_chr10_120694322_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm24298|4921513I03Rik_chr10_120694322_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Foxc1_chr13_31807917_F TCGTCGGCAGCGTCGCGCATCCAGGACATCAAGA 60.4 0.00
mm4_exon_Foxc1_chr13_31807917_R GTCTCGTGGGCTCGGCTGTCGGGGCTCTCGATTTT 60.1 0.00
mm4_intergenic_Nckipsd|Celsr3_chr9_108826276_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Nckipsd|Celsr3_chr9_108826276_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Rsbn1_chr3_103914878_F TCGTCGGCAGCGTCGTCCCGGGCCCTGTTGAA 61.9 0.00
mm4_exon_Rsbn1_chr3_103914878_R GTCTCGTGGGCTCGGGAGGCGAGAAGGTGAAGAGG 59.8 0.00
mm4_intergenic_Gm14060|Btbd3_chr2_138468354_F TCGTCGGCAGCGTCAGCTGACCAACCAACTCCAG 59.8 0.00
mm4_intergenic_Gm14060|Btbd3_chr2_138468354_R GTCTCGTGGGCTCGGGTACCTAGCTGTCGCGGTC 59.9 0.00
mm4_intron_Myl12a_chr17_71002070_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Myl12a_chr17_71002070_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Slit1_chr19_41685610_F TCGTCGGCAGCGTCCCCTGCCCAATTAACACCCT 59.9 0.00
mm4_intron_Slit1_chr19_41685610_R GTCTCGTGGGCTCGGGAAGAGGAGGCAGGAACACC 60.0 0.00
mm4_intron_Mrvi1_chr7_110963006_F TCGTCGGCAGCGTCCAGTTCCCACCCCAGCTTC 60.3 0.00
mm4_intron_Mrvi1_chr7_110963006_R GTCTCGTGGGCTCGGTCAGGAGATAGAGGCCAGCA 59.7 0.00
mm4_intron_Dapp1_chr3_137977797_F TCGTCGGCAGCGTCGGAGGAGTAGACCTAGCCGT 59.8 0.00
mm4_intron_Dapp1_chr3_137977797_R GTCTCGTGGGCTCGGCCTCTTCAGTGCATCAGGGG 60.1 0.00
mm4_intron_Cyth3_chr5_143694353_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cyth3_chr5_143694353_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_n-R5s30|Rbfox1_chr16_5495572_F TCGTCGGCAGCGTCAGGGTTCCAGGTTAGGGTCA 59.8 0.00
mm4_intergenic_n-R5s30|Rbfox1_chr16_5495572_R GTCTCGTGGGCTCGGGCCCCTTTCATCCTGAGGAG 59.8 0.00
mm4_intergenic_Sbno1|Setd8_chr5_124426415_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Sbno1|Setd8_chr5_124426415_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pabpc6|Qk_chr17_9928012_F TCGTCGGCAGCGTCACCCTAAGCTGTTTCCCGTC 59.6 0.00
mm4_intergenic_Pabpc6|Qk_chr17_9928012_R GTCTCGTGGGCTCGGTTTGCTCTCTGGAGGACGC 59.7 0.00
mm4_intergenic_En1|B230209K01Rik_chr1_120942147_F TCGTCGGCAGCGTCTGTGTGCAATGAATTCACAGAACA 59.9 0.00
mm4_intergenic_En1|B230209K01Rik_chr1_120942147_R GTCTCGTGGGCTCGGCTGGCTTCTGTTGGAGGGTT 59.8 0.00
mm4_intergenic_Cdc7|Tgfbr3_chr5_107044947_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Cdc7|Tgfbr3_chr5_107044947_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_3110047P20Rik|Gm3687_chr5_63656245_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_3110047P20Rik|Gm3687_chr5_63656245_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Tnk2_chr16_32668298_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Tnk2_chr16_32668298_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Pcbp1_chr6_86525964_F TCGTCGGCAGCGTCTCACGTTGAGTCCGCTTTCA 59.8 0.00
mm4_exon_Pcbp1_chr6_86525964_R GTCTCGTGGGCTCGGCAGTTCGGGCCTACTCCTC 59.1 0.00
mm4_exon_Insm1_chr2_146222501_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Insm1_chr2_146222501_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ica1_chr6_8778337_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ica1_chr6_8778337_R Primer3: not found at this Tm N.d. 0.00
mm3_intron_Tbc1d5_chr17_51179033_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Tbc1d5_chr17_51179033_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314013 AACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGCCGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGA
GGC
CAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAG
mm4_intron_Fam186b_chr15_99285113 CCTGGCCTCTTCCTGTTCTCTGCTTTCTGGTTGCATGAGTGGAGCAGCCTCCTCTGCCACAATCCCTGGGACTCACTCTC
ACCATGAGCTAAGTGATAGGGCCAAGAGATCACCACTG
mm3_intergenic_Tenm4|Gm15414_chr7_96559034 GGGTATCTCCAGGGCATGTGAAGAGAGCGCTTACCAAGTCGGCTTGCTTCACCCTCCACGCTCCCCGGCCCCAGACTCTT
GAGAGTTATTCTTACCAAGATCTAGAAGAAAAACTGTCAGGAAGCTCGAAATGT
mm2_exon_Clu_chr14_65974704 TGGACAGGGAAGCTTAGGGAATGGCTGGGCCTTGAAGAAGGAGAGGCTCCTGAGAGCGTGGCTGGGGAGGCCCAACAGAA
GGGCTAGAGCG
mm4_intron_Mat2b_chr11_40692368 GACTACTGAAACGGGAGCCAGCCCGCGGGAGGCGGCGGGAGGCACCGGGAGTCTCCACCTCCGCGCTCCCCGGGCTGCAG
CGGGCGTGAGCGCAGCCCTACAGGGAGAGAGGTAAACAAACTCAAAGGCGCCAC
mm4_intergenic_Uncx|Micall2_chr5_139613841 CCATGTCTGTCTGGCACCTGCCCCAGCTGCCTGGGTGCATGGTGGGTGAGGCTCCGGCTAGATCCTGGGGACCTGGTGGC
AAGGGAGGGCTGGGCTCTGGCTCTGCCTGCTAAGAGGGGTTTG
mm4_intron_Tjp1_chr7_65370531 GGTACCAACCACTCCTCCAGAGCCAGCCCCGCGGCTACCCACCCACCCTCCTGCTGCAGCTGACACCCCCGGGTCTCCGC
AGACACGCTCCCGGG
ACCTGAGAATCCTCACCCGGGG
mm4_intron_Cux2_chr5_122030644 CTCCGTGAAGGCAGACAGCGAGTCTACTTTGATCACNNNNNNNNNNNNNNNNNNNNNCCTGGGGGCGTGGAGGGGGCGTC
CCTGGTGTGTGGCTGGCTGCCTCGCAGTGAGGAGATGCAA
mm4_exon_Hck_chr2_153136741 TTTCCCCAGCCACCTACAACAAGCACACCAAAGTGGCGGTGAAGACAATGAAGCCAGGGAGCATGTCCGTGGAGGCCTTC
CTGGCTGAGGCCAACCTGATGAAGTCGCTGCAGCATG
mm3_intron_Impdh1_chr6_29215976 GCAATGGCAGTAGAGGGGACCTGCCTCTGGCCTCCCCCGCAGCGCTACCCGGTCTCCCGGCGTTGCCGCGCCCTTCTCTG
CCGCCCGCCCCGGATGCGCCCGCGCGCCTTGCCCTGGGCGTCACCTCTCCGGCTCGTA
mm4_intergenic_Gm11468|Gm14268_chr2_166318552 TCAGGTTCGGAAAGACCACGGCCTCTGTTTGACGGTTTGAGAGGCATCTGTTTGCCCACTCTGTTTCTGACTTCCCCACC
ACGCTCTCTGG
GGGTCACTCATTTTCCCCTTTGTAGTCCTCCAGGCTCACA
mm4_intron_Ahdc1_chr4_133016462 GTCAGGCTTCCAAGAGCTGAGGATGTGAGAGCCAGGGAGCTTGGAGGGAGAGGGCAGGGAGGGCTGCTCTGAAGGGGGAG
AGAGAAAGAGCCCAGCCAG
mm4_exon_Slc16a2_chrX_103707915 ACTGAACCAGAAAGCGCTGTCGCAGGGTGTGGGCACCTCTCTTGCTTGGGGTGTCCTGGGAGCTTGGCAGGAGGGGCCGG
TAAGTGAGTGAGAGCAGCGTAAGGACA
mm4_intergenic_Fam46a|Gm25125_chr9_85469361 CATCCCCTAGCACACAGAGGGAATTATACAACGGCTGATAAAGAGAATTGTAAACTGTGCCTCCCCCACCACCACCCCAG
G
GTTAAAATGGAAGGAAGTTGAGGAGCAGTGACCCAGCTCCTACAGAAAAGCCGCTCC
mm4_intron_Rem2_chr14_54476545 GGAGACCCCTGCACTTTTGAGGTCCGGACGGCCAAGGGGAAGGGGGAAAGCTGCCAGGGTGCCCCTCCCCCAACGCTCCC
AGG
GCTTCGCCAGAGAAACTGGCACTTGGCCCCTAGGTTAA
mm4_intron_Zcchc11_chr4_108459714 ACCTCCATCCCCTCCTCATTGTTTCCGGGCGACCGCCACGAGCCCCGGAACGAAGCCCCTCCCCCCACGCCCCCCGGTCT
ACCCCCTCCTCCGCCGAGGTTTGACGGGTGCTTGG
mm4_intergenic_Lrrc55|Gm13713_chr2_85163729 GGGATGGGCAATGTGGAGAAGCCAGGGAATGTGGAGGGGGAGGGGAAAACCCCTAGCTACTGGGACAG
mm4_intron_Scaf8_chr17_3115619 AGGGCTGAGGGAAGGCAGGGGGAGGGCCGGTCCCCAAGGCGGCCGCGCCACGCTCCCGGGTGGGTTCCACCGAGGACTAT
CACCTCCCACCC
mm4_intergenic_Gm23534|Gm22727_chr1_137671266 TGGATGGGTACGCATTGCTTCTTGTCAACTGCTTCTGCTCCACCAGCTGTGTGGAGCACAGCTAACTTCCAGGGAGCTTG
GCAAGGCAGGC
ATTCAGTTCGTTCTCTGGGGA
mm4_intergenic_St6galnac5|St6galnac3_chr3_153064142 CACCTGGACACTACAGCACGCCCCCCCCCCCCACACGCTCCCTGGGGGCAAACTTAACATGAAAGAAGATCAGACTGTCT
TCCCCTGCT
mm2_intergenic_Olfm1|Gm13373_chr2_28290288 ATGCACCTGCTTAATGCCCTAATGTCAGAGCAAATAAATATCCTTGGAGCTGCCCCTCCCCCGCCACGCCCCCCAGCGGC
TGTTCTCCTGAGCCCTGCATGTCTTT
mm4_intergenic_Gm5083|Gm27007_chr13_44374757 AGTGCACAACTCCAAGTGGGTAATGCCTAGAGCTGTCTGCCTCCCCCACCCCACCCCCAGGAGCCGAGTCTTCTGCCACC
CTGTGAAGTCAT
mm4_exon_Cnnm1_chr19_43440894 GACGTGGAGGTCCTGGGGCCCCTGCGCCCCGGGGGCGTGGCGGGCTCGGCCCTGGTCCAGGTGCGGGTG
mm4_intron_Eif4enif1_chr11_3227569 TGGCAGGTAAGATGACTTCACATACTAGCCTTGTATGTCAAGTGGGCCTCCCCTGCCATGCTTCTAGGGTTAAAGAGACT
TTAGATATGGAAAAGCCTTCAGTTTCTATTCTCTTTATGCTGTGGAAGGGAAGTTTTC
mm4_intron_Afap1l2_chr19_57006846 CGACCTTGGAAGTCGGGATTTCCAGTGAGGAGACGCCCAAGGCCCCAATCGGCTCCCCCTCCTCGCCCCCAGGTTCCCTC
CCTAAGTGGGGAGCAGCAAGAGCCGCCTCTCTTCTACCAAG
mm4_intron_Tmod2_chr9_75601800 TCAACACGCAACAGGATCCTAAATCACACCCTGGGATGGAGCACGTGCGCCCCCCCCCCCCCCACGCTTCCAGGAGCCCA
GGTGGGCAAACACAGAGCCCATTAGCAAACTGGAAACTGGGAGTGACAGCGA
mm4_intergenic_Bnip1|Nkx2-5_chr17_26828086 GGGCTCAGAGGCTAATTGCTTGCCTCACCTGCCACAGTCCCCGGCTCAACCTGCACTTGACTCCAGACCGCAGGTCCCAG
AGGCTGTGGATGAAAGAGCAGAAAATGAATTCCAGGTCTTGATTTCAGCCCACAGCCT
mm3_intron_Fblim1_chr4_141599204 TCCTCAGGCTACCGGTCTTTTGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGAGGCCAGCTGGTCGTTCAGCCCCAG
mm4_intron_Caln1_chr5_130618015 TGAGACCTGCTTAGCACTGCCAGGCTGTGCCTCCCCCACCACATTCACTGGGCCTGGGATTTATACAAGTCTCACACTCA
GAGCCCT
mm4_intron_Sez6_chr11_77960122 CCAGCAGAACACGATCAGGTACAGGTTAGAAGTCAAGCTGGAGATACAAGAAACTAGGAAGAAAATTCTTCCTTGATTTC
AACCCCCTGCCTCCTCCTCCACCCTTCCTGGAGGCACTCTGAGAGACCCACCTG
mm4_intron_Prpf18_chr2_4639984 ATGCTGAGCAGAAGAGCCAGGGAGCAGGGCTGGGGATGCTGAGCAGAAGAGCCAGGGAGCAGGGCTGGGGATGCTGTTAC
AAATCTAAAAATCCAAGGAGCTAAGGAGC
mm4_intron_Ctdsp2_chr10_126979703 CCGGAGTTTTCACGCAGGACCGGGATGCCCGGGAGCTCGGCGGTGGCGGCGCGGGGACTCGGGACGAAGTTCAC
mm4_intergenic_Wnt4|Cdc42_chr4_137315009 TTCAGTCATTACACGCCCCGCCTCCCCCGTCCCCCTTACCACCTCCCTGCCCCGCCAAGCCCCCAGGATGGACTTGGAGT
CAACATACCCCTTTTCAGTATTTACCAACATGGCCTTGCTAACT
mm4_exon_Usp49/Gm20517_chr17_47672699 CTGGAGGAGCTGGCCAGTGCGCCGCCGCGGAAGAGTGCGCGCCTGCTCCTGCACGCTCCCGGGCCCGTGGCCGTGCGCCC
TGCCACCCTCGCTACCTC
mm4_exon_Ephb4_chr5_137372201 GTCAGCGCTCTGGACAAGATGATCCGGAATCCCGCTAGCCTCAAAATCGTGGCCAGGGAGAATGGCGGGTGAGGATCCCA
AAGGACAGCTCCGACCCACAACCCAAGAGTCC
mm4_intron_B230219D22Rik_chr13_55693576 AGTCTCGCCCTGGAGCTGCGGTCGTCCCCGCATCCCGAGAGCGCGGCGGGGAACGCCTCGGGGTCCCGTTAGGATGCTGA
CTTGGGGAAGCGCCGTGGGGCACGGCTAAGGAGCGCAGAGAAGA
mm4_intergenic_Taf1b|Grhl1_chr12_24566625 GTTGCTCACAGGGGACTCAAAGGTTAAGCAGCTTCCTACATCTCAAGCTAGAGAAGGTGGGGAAAGTCAAACTCCGTTGC
CTCCCACACCCTGCTCCCCGG
AGTGGAAAGTCCTTCATCTCTGGCTGGC
mm4_intron_Lrrc41_chr4_116075661 GGGGTAAGTCGCGGAGTGAGGGGCGACCTCCGGCAGCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGC
CGAGAGGCCCGGGGCCAGGCCCAGCTGGGAGCCCCGGGAGTTCGATGGTGGAGTC
mm4_intron_Fosl2_chr5_32152046 ACAAGTCAGGTGCTGTCTGTTTCAGTCCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAGAGGGCTGTCATGGGGGTC
TGCTCTTGTGCAGCTCAGGCTGTGTGCGCTGGTGGGCTGAGATGGTAA
mm4_intron_Ndrg2_chr14_51911843 CCAAGACACACGGAGAGCTTTTCTGGCTCCCCCGCCCCACTTCCAGGGCAATACCAAACAGGACAGCACATCTCTTCAGA
CCACCGGCCCCATGGCTGCCGGCTGCCCCTCAACCCCAACATTCTCACCTCG
mm4_intron_Igf2bp3_chr6_49117016 TGTGAGACCAACAACGCATGATCAGGTGACAAGGCTTCCCCTGCCACAGTCCCAGGTACTGTAGAAGAGAACTCCTGGCT
TTGTCCAGAGAGACATCAAGTTCCTGCATCCAACCCTGA
mm4_intron_Glipr2_chr4_43972426 CCTTAGGTGCAGATCTGGGACATTTCTGGTTCAGTTAGCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
GG
TTGCTAGGCTGAGGAGCAGCTAGCTGCTGCCTGAGCTTCAGATGGCAA
mm3_intergenic_Gm15998|Efna3_chr3_89320929 TCTCACGATTTATGGGGCCGCGGATGCCGCAGTGAGAGAGCCGGGGAGTCCGGGCGCAGCGGCAACAAAGGCCCGGGAAG
CGGGGCGGGGGCGGC
AGCGGCCTCCTGGTCCGGTGTGCGCCGCCTCCTACCTCCGTCGGT
mm4_exon_Tmem82_chr4_141618102 GGGAGGGACTAGGAAAGGGTCAAAGGCCTGGGGGCGGGGCCGGGGAGACAGCTGCCTTAGATCTTCCAGGTTGCTGGATT
CAGTGGCACT
mm4_exon_Anpep_chr7_79842096 CATCTACAGCAGGGGTGGTGGTTGCGGTGGTGGCTGAGGTGCTGCCCGGGAGCGTGGGGGCTGTGGCAGAGTTCTCTGCA
TTCCTATTCTTCTCCTGAGCGTAGACCACCGACAGAGCTATGATGGTACACACAGCTGCC
mm4_intergenic_Gm24338|Ppp2r3a_chr9_101251415 TCCTGTTAACGAGTTCCCGGCCCGGGCGTCCCGCGAGGTGGGAGGCGCCGGCCATCCGGCCCCCTGGCCTCCTCCGCGGA
GCTCCCGGG
CCACCGTTCTCCACGGCCGCCAGCCCCGGGAGTCCTCTGGCGTGG
mm4_intergenic_Ccne1|Gm22203_chr7_38128470 TAGTCATAGGCCTGGGAGCTGGTGTCCAGCTGGCCCCACCCACCACTCTCCCTGGCTTGTGGTATCCCGTGAGTGTGTAG
AGGCCAG
mm4_intergenic_AC124326.1|Mir802_chr16_93087459 AGGCTACAATGTGGCGAGAGGCTCAGCCTGATTCCTCTAGCCTGGGAGCGGGGTGGGGTGGGCTGCCCTTCTGAGGTATG
CAGAATATAAATGCTCTCATTTGGTAAGACAGGGAGACTGGGACA
mm4_intergenic_Gm5356|Gm26331_chr8_89242210 GCAGTGGCTTTTGAACCGAGAAATGGAACAAGGTGTTAGATTNNNNNNNNNNNNNNNNNNNNNNAGGGAAGGCTGTAACT
AGTGGGCTTAATCCTGTCCAGTTAGCCAGGGAACACC
mm4_exon_Phf1_chr17_26933422 CTTTGGCTGCTGCGTCATACGCCCCAGAGCCGCCAGGACGGAGGGGCTGGGCCCGGGGACCCCCTGGCCTCCGCCTGCAC
GCCCCCCGG
CGCTCACACTCAGTCTTCCC
mm4_intergenic_Metap1|Eif4e_chr3_138489657 CTGACAGCCACGCCCTCTCCGCGCACGCGTAGTGGCGACCCGCCCGGCAGCCTTTACGCCGCCTCCCCTGCCCCGCCCCA
AGG
CTTGCGGTGGCGGCGACGCCACGTGGGGTCAGCGGAGATCCTACAC
mm4_exon_Drap1_chr19_5424797 TTCTTGCTTGGCATCTCGGGGCCTCTCGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCCTCCCCGGGTCCTGGTGCCGTTTACTCC
mm4_intron_Ccdc33_chr9_58113454 CCGTAGCAGGTGGAAGCTTTGAAAGACCAGAGAAGCAGGTCGGCACGGAAGGACGGGGGACAACAAAGAGGTGCCCAGGC
CTCCCTCCCCACCCTTCCAGG
CCAGCCCCCTGAGAGTGAATGGGCAG
mm4_intergenic_Gm23624|4930593C16Rik_chr9_120846292 ATCCCAGATAGCCCAAGGGTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNN
NNNNNNNNNNNNNNNNNTGCTTGGCCATAGTGTGGACTGTGGCCA
mm4_intergenic_Gm14236|Gm24327_chr2_168034059 GCTCCCTTTCGCTGTCCTTCTCCTCCCCCACCCCGTTCCCAGGCTTGCTCGACAGAACGAAGCCAGAGGCCAGGCTGAGA
TAGCCTCAAGGCCAGACACTAAGATAAGAGCACCTGCAGCCAGCAGCCTACAGAAAGCT
mm3_intergenic_Rragc|Gm12916_chr4_124291608 CCCCGTTGTCCTTGAGTGAGTCTTTCCTTTTAGCCTCCCCCCCCCCGCCCCCCGGATCTGAAGGCAAGCTAAAAAACCAA
AATCCAAAGTTTCTCATTTCTCAGATACCCACTTATCATTTGCATCCCCACCTCC
mm4_intron_Chd6_chr2_161017889 CCTCGCAGTTCCAGGTTACAAATTCCATGTTATTTTAGTGCTTTCTAGCCTCCCCCCCAACCCCCCCAGGGAATCAATCT
GTGGCACAAAGGATGTGGATGTGCACAAGGCCATGGGATACT
mm4_intergenic_Kcnc4|Slc6a17_chr3_107459057 CTTCTGCGGAGTGGCTCTGCCCCTGCCTCCCCCCCAACCCCCCCGGGGCGCGGAGCAGGAAGAGTTGGCTT
mm4_intergenic_Gm23186|Arid1b_chr17_4763300 AACAGGCAGAAGCAACCTGTATGGACCTGGGGGGGTGGGGGGGGAGGGTAAGCTTTCTTTAAAGTCTTGAGTGCCTGAAG
AGGAGAAACCTGTGGAACCAGGCCTCTTTC
mm3_intron_Sez6_chr11_77931461 TCCACTCCAGGCGTCTAGGGGCGCCCGGGCGCGTTGCGGGGGGGGCGGCAGGTCGCGGGTGCAGAGTCCGGACAGCCCAG
CTACCAGCCCGCCGTTCTGCCTACCCCGCTGGTCTCCAAACTTCGCTCGA
mm4_intergenic_Gm26626|Runx1_chr16_92634574 TGCTCACCCATATGCATCCCCCCCACCTCCCCCCCCCCGCCCCCTGGAGAAAGATGTATGGGGAGATGGGGAGTGTCAGG
CACTATGAAAAAGAAGGAGTGGCCCACC
mm4_intergenic_Spty2d1|Tmem86a_chr7_47008600 GCGAGAGAGGGTTTCAGTGTGAGATCCGGGGAGGGTGGGGTGGGAGGGGGGGGGGTGGAGAAGAAGAGAGACCCGACCCC
CTTCTAGAGCTGGGCTCTGTAGGTGTCACGCGTGGATGTACAGA
mm4_intergenic_Gm5616|Rexo2_chr9_48468338 ACGTGGTGGAGAGTGGTCTATCCCAGAGCTTGTTAGCTGGGTAATGATGAGCCCAAATCTTACAACCTAGCTCCTCCCCC
TCCACTCTCACGGG
TAAAGCAAAAGAAATTTAAGTTTACTCCCTGTTCGAAACACACG
mm4_intergenic_Elk3|mmu-mir-7688_chr10_93376680 TCGGAGTCAACATTCCCCAACGAATAGAGTAACTTCAAGGGTGTGATGATAAAAACAGAGATCGGACTGAATTCTGTCTC
CAGGGAGCGGGGGGGGGGGGAC
AGAAATACAAAGGCAACTCAGGACAAGAGA
mm4_intergenic_Smug1|Cbx5_chr15_103177359 AGGCTCGTCCCATCCAATTGCTCCCCTCCCCCGCCCCGCCCTCCGGGATCCCAGGAAAGGCTGTGATAAGAATGAGACGG
ATTCTCCACTGGCCCTGATCCCTCTACAGA
mm4_intron_Tiam1_chr16_89832645 ACCGAGGAAAAGGAGCAAGGCGGGGCAGACTCCGGGGAGCGAGACGGAGAAGGCTAAAGTACGATTCAGCATGCCAGGAT
CTGATGCGATC
mm4_exon_Frmd4a_chr2_4601199 TTACAGTCGCCACACAAGGGACTCCCTCCTCGGCCACCTTCATCACACAACAGGCCTCCTCCCCCACAGTCCCTGGAGGG
ACTCAGGCAGCTGCACTATCAC
mm4_intergenic_Thbs4|Mtx3_chr13_92794858 CCCGCGCTTTATGGTCCANNNNNNNNNNNNNNNNNNNNNNNNAGGGTGCGGGGGAGGACCGAGTCAGGGCGGCCCCCTTG
GCCAGGATCCCCCATTTGCCAGGAAGAGC
mm4_intergenic_4930445N18Rik|Zfp516_chr18_82873952 GAGCCAACAGAGCTGACACTTGTCCAGTCTAGACCTTGTGGTCCCCTGGGGGCGGGGGGGGGGAGGGGGGGCATGGGGCA
TTCCTGTGGAGGTAA
mm3_intergenic_Prima1|Fam181a_chr12_103242696 GGAAAACAGACGTGAGCGGAGCCAGAGCCTCGCCCGCCTCCCTCCCGGGCTGCACTCCCGCCCGCGCTCCCCGCTGCCGC
GGCCGCCAGCGCCGCTGCAGTGATTCCTCGTCT
mm4_exon_Myo16_chr8_10634659 TGTCTTTCTGCCCACTGTCCGTGAGTTCTGAGCAGTGGCTCCACCAGCCATCTGCCACCTCCCCCGCCCCGCCCCTTGGA
CTTGTTCATTCATTTCCGCCATTGCC
mm4_intergenic_Gm23752|Gm24815_chr12_7171843 ACGGAATGCTCCAGTCCTTGACCAATGCTCTGTATTGAAACTCCAGCCTCTCCCCCCTCCCCCCCCACCCTCCAGGGTCT
CTTTGCCTAACTCTGTCTCTCACCATCTCCCA
mm4_intron_Spata21_chr4_141097164 CCAACCCTGTCCAGCTCTTCAGCTAAGCCAGGGAGCNNNNNNNNNNNNNNNNNNNNAGCCAGCGCAGCCCATGTGCAGAG
ATTAGGAGCGG
mm4_intergenic_Gm10782|Slc25a48_chr13_56432662 GCTCCAGCCCATCAGTATCCTGCCCCACCAGGGAGCAAAGGACTGCTGATCANNNNNNNNNNNNNNNNNNNNGCTCCCAG
G
CCACTGCGCAGGCGTACCGTGGCTCCGCGCCATCTTCCCACTTGCTA
mm4_intergenic_Crispld2|Gm15684_chr8_120041715 GCGCTATGTGATGGGCCTAGTGAATCCTGTAAGTCTCAGGAGCTTCCTAAGCCTTGTAAGGCTCATTTCTNNNNNNNNNN
NNNNNNNNNNNAGG
AGGGAAAGTTGTGATTGGGAATCAAAAGCTACAGCAAAGCGGC
mm4_intron_Nedd4_chr9_72662662 GTTCATCGTGGCGAGGGGTGGCCGGGGTGGGTAGCGGGGGTGGCGGGCGATCGGGGCCCGAATTTGGCCCCGCGCTCCGG
AGCGGGGCAGGCGGTGAGGGGGAGATGGAGAGAGGCG
mm4_intron_Osbpl8_chr10_111165438 AGCGGTACAAAGCCCAGTGGGGACCTGGGATGGGGGCGGGGGCGGCTCGGCGGCGTTCCGGGTCCCGAGTGTGTCTGCCC
CTCCCGAAGTTTGACAG
mm4_intergenic_Gm11650|Cacng4_chr11_107794160 TTCTTTGTGTCTCGCGTGGAGCCAGCGCCGCGCCCACCCCTCCCCCGGCCCACTCCCCGGGGCAANNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNTGGGGGTAGGGGTCCTACCTTCGATGCAA
mm4_intergenic_Adamts6|Cwc27_chr13_104510294 GAGGGACAGATGTGGCTTCCCCGGTCAGCGTCCTCTGCTGGAGGGAAGGAGACACCAGACACCCCTGACGCGTGGTGGGG
GAGGC
TCACCCCTGGGGCTCTGCCATCTGGACACTGG
mm4_intergenic_Tacc3|Fgfr3_chr5_33689460 TGGGCAAATTCAGAAGGGCTGGGGGCTCTTGTCAGGCCTGGGAACGATGCGGGGGTGGCCATGGGGTGCGGCCAGGGCGG
ATCCATCCAGGGCTGGACTCAAGGGACTC
mm4_intron_Mpped1_chr15_83846661 ACACCAGGATCCAGGTTCCTTCTCGCACAATGGGCACACTCTGCCCCTCCCCCCCAACCCTCCCCCGCCTCCCACCCTGG
GCTGGCTGGCTCCTGAGAGGGTGAGGATGATTAGCCAGGGGC
mm4_exon_Anks1b_chr10_90829454 GAGCTCGTGGGGATCCGGCGCCTCTCCCACCACCCTCGCCGGCGCGCTCCCTCCTCTCCCTCGCTTGCTCACGTTCACTG
GAGGGGGCTCTGCGGGGAGGAGGGGGAAGATGCTTAGAGGCGACGGCTAAGG
mm4_intron_Afg3l1_chr8_123478077 CTGTGGCATTGCTCTGCAGGTGAGCAGGGCAGCGCTGGGGCCACGGGTTCCGGGCGGCGTGGCGGGGGCCGCGCTGGGCG
TCGCGGTGACGTCATCGGCCTGCGGCTGGGCCGGGCGCAGCGTCTTCCCGATTTCTGCCT
mm4_exon_Pcolce_chr5_137610682 CAGTCCAAGGACCACGGAAAGTGGGGCTAGGCAACCGCCCCGTCTCTCCATCCCCTAGAGGCTCCACCCCCACGCTGCCA
GG
AATTCCGGGAATCCTTCTCAGTCCCCCTAACCTCCTCTACCCC
mm4_intron_Slitrk2_chrX_66652911 CACTCACAGGGAAGGGAAGCGTCTGCCCCACCCTCCCCCACCAAGCGCCCTGGATACACTGCCCACACTCCTCTCCTCCG
TGACGTCACCCCTAGTCCTGTCCCGAGGAGCCTGCAAAACCTCT
mm4_intron_Elf2_chr3_51276961 CGACTCCCCAACTTAGCCGCGGCTCCGCGCCCGGCCCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTCCCC
GG
AGCGACAGCCCGGATCCGAGGCAGGTTACTC
mm3_exon_Snx27_chr3_94582441 ATTCGGACTTGACAATGCGCACGACCCGCGGGCCGCCGCCTCCCCCGCCACCGTTCCCGGCGCAGTGGAGCCCAGACCCC
CCGCCGCCGCCTCCATTCCGGTGCGGGGCTGAGGGGTGAATACCTTCCCCGTCCTCGT
mm4_intergenic_Gpr108|Trip10_chr17_57248622 GGCGGAGAAAGCACCTACAGCCAGGGAGAGTGGGGTGGGAGCCAACAGGGCCTCTTCCCTTTTATCCGGGAGCCTTAAGG
GGGTTTACAAGGCGTGAAAGGACTTTTATGATTGGGAGGACCCGAAATCCTAAGCCCCAG
mm4_intron_Gm11100_chr14_13937637 GTGGACAGATCCCAGTGTGGCCAGCACCAGGAGCACTCACCTGTGTTGTGCCTCCCCCACCACTCACCTTGGCATTATGC
TATGAAATGTTTCTTGTTTCCAAAACATGTATATCCCGCTGTTCTCTCTTGTT
mm4_intron_Mrpl48_chr7_100572247 CAGGCTTCAGCTCCAGTTCAGGCTCCCAAAGGTGTCCAGGCTCTTTTAAGGGCCCCAGGGGGCTGGGGGGGGGAGGCTTC
TATCTGCCAATGTGAAGACAGATGGACTGCTCTGACACACC
mm4_intron_Bmp6_chr13_38394704 GGTAGAGTCCAGGTGCCATGCAGCCCCCCCCCCCCAGCTCCCTGGGTTTGAGGCCTGATTGTGTAGGATTGGTCTTCCAT
GCCCTGGAGACAGCTGCCAAGCTAGTGTGGTGGGTT
mm3_intron_Jarid2_chr13_44730343 CTCCAACCCCTCGCTGACANNNNNNNNNNNNNNNNNNNNNNNNACGCTCCGAGGCCTTTCCCCCGGGAAGCCTGGCGGCT
TGCCCTCGCGGGCCCGCGGGCGCGCTCTCGCGCGCCGATTCCTCGATTCCACAGGCG
mm4_exon_Diras1_chr10_81019769 GTCCCCAGCACCAGAGATGCCTGGGCAGGTTGGCCAGCAGCCAGGGTGGGCTCCGGCCTCCCCTGCCACCCAGCCCGGTT
CTCCCCAGGATGGGCAAGGCCAGCCAGGAGAAG
mm4_intron_Sirt1_chr10_63339100 CTCGGCTGCGGGAGATTTAAACCCCGTCACGTGACCCGCCCCTCCCCGCCCCCGCGCCTCTCCCGCCACCCACGCGGGCC
GGACCACAACACGCCGGGTCAC
mm4_intergenic_Nrg3|Gm10713_chr14_39473278 TGTGTGCGCTCCAGCCTCGCCGGCATCCTCGGTGCGCCGCGCCCGGCCCGCTCCCTGGGGCGTCCGGCCGCACCGCCGCT
GTCCCCACCTCAAATCCTCACGT
mm4_intron_Nrg2_chr18_36037082 GCCAAGGCAGTGATAAGGGTGTAAGATCTGACCTGGGGGGGGGGGGGGGGAGGCAGAGGCAGGAAAGCCTGGTCAAGACC
mm4_intron_Klf10_chr15_38299855 TCTGCAGGCTCCCAGACGTGCGGCGCCCACAGCACGGGCTCCAGGCGCGCGGCCCCACGGTTCCCGCCTCGACACGCGGC
CCCCGCGTCTCGCGGTCCCGACTCCCCCGCCAAGCTGTCGGGCTCCAAGGCAGGACCCTG
mm3_intergenic_Asphd2|Sez6l_chr5_112395407 TCCTTGGAACAGTGCATGGGCGTTCTGGGAGCGTGGGGTGGGATGCCAGCCCACAGCCCATCTGTGTCTGTCCTC
mm4_exon_Arl4c_chr1_88702220 TCTAGTCCCGCCCACAGGCCACGCCCCTCGCCCGCCACGCCCCGCGGTCTCCCGGGGTCAGGCGGGGTCTTCTCGGTCGG
TTGCGTCTTTTCGCGCGCTCCGATCGTTCGAAACTCGCCCA
mm4_intron_Crtac1_chr19_42310505 CCTCACCTGCTTCCTGGAAAAACCACCCACATCTAGGAAGCCTCACCAGCCACGGGCCCTGGATGCTGGCAATTGTTAAG
ATACCCCACAAAGAACTAATGCAGATTCTCACAAGCTGCTGGAGT
mm4_intergenic_AY702102|A630075F10Rik_chr2_169997009 TCATTACTGGAGGCGAGGGACCTGGGCGGGTGGCGGGGTAGGGGTGGCTCTGGGAAGAGAGAGGA
mm4_exon_Cacnb4_chr2_52676381 CGTACGAGGACGACATGGTTCAGAGGCGCCGCATGGCCCTCGCAGCGCGGTGGGCGGAGGGCGCGGGCCGGTGCGGGTGG
AGGGGGAGGC
AGGACTTCCCTACCCGGCTCCT
mm4_intergenic_Pard3b|Gm11610_chr1_62441938 GGCCAGGCATTTCAACACTCACAGATTGAGGACCACGGAGGGTGGAGGTGGAGGCTAACCGTGTGGTGTTGTTCCCAACC
CTGGAGTACTGG
mm4_exon_Inpp5a_chr7_139389380 CCACCCAGTCCAGAGCCCGCTCCGCCCGGGCACCATGGCGGGGAAGGCGGCCGCCCCCGGCACCGCGGTCCTGCTGGTCA
CGGCCAACGTGGGCTCGCTCTTCGACGACGTAAGTGCC
mm4_intergenic_Ccne1|Gm22203_chr7_38130150 GGCCTCTTCAACAGACTGCTAATGGGAAATGGCCCATGAGGCTCCCCTGCCACGCGGCCTGGGGCACACGCAGGAGTTAT
TAAATAATACGGCCGCGTGGAGAGCAGGATCGAGC
mm4_intron_Sh3glb1_chr3_144719804 GGTGAACCCAAGGTGTCCGCCCTGGGCCGCGTCCCGACTCGCCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGCGAG
GCGGAAACGGCCAGCGGGGCAGGGGCGCCAGGCCGCGCCCATCGGCAGGCCTGGGTA
mm4_intron_Adamtsl3_chr7_82396989 TCCTGTGAAGAGGACTGGGTTCTCCCCGAGGATTCATAAGGAAGCTGCTCCTGGCAGCGGGGGGGGGGGGGCTGAGCTCT
CCCAGGACACAAGAGAGGCCAG
mm4_exon_Plod2_chr9_92542467 CGTCTCGCGAGAAGTCCTCGCCACAGGCCTTCGGCTCCCGGGTTCAGGGGCGGATGGGGGACCGCGGAGCGAGGCCGGGG
CGGC
TGATGCCCATGCTCGCCCTGCTCTC
mm4_exon_Tfap2c_chr2_172549709 CACATCTCTGTGCACAGCCACCGATGCGCGTCCAGTGACTGGGACAGCAANNNNNNNNNNNNNNNNNNNNNNNNNNNNAG
ACGCCTGGCCACCGTGACCCCGATTGTGGATTTACCGCTCGGG
mm4_exon_Foxc1_chr13_31807917 GCGCATCCAGGACATCAAGACGGAGAACGGTACGTGTCCCTCGCCTCCCCAGCCCCTGTCCCCGGCTGCCGCCCTAGGCA
GCGGCAGCGCCGCCACAGTGCCCAAAATCGAGAGCCCCGACAG
mm4_exon_Rsbn1_chr3_103914878 GTCCCGGGCCCTGTTGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNGGCACCTCGGCCCTCTTCACCTTCTCGCCTC
mm4_intergenic_Gm14060|Btbd3_chr2_138468354 AGCTGACCAACCAACTCCAGTACGGGCCCCACTGCCTCCCCCGCCCCTGACCCCGGCGGCAGAAGGGCGATCCCCCCGGG
GGTCTTTAAACCTCCACTCCGGACCGCGACAGCTAGGTAC
mm4_intron_Slit1_chr19_41685610 CCCTGCCCAATTAACACCCTAGAGGATGCCAGCAAGCAGCTGCCTCGGCAGCCAGGCTCCCTGGGGAGGGGGGCCCAGGT
GTTCCTGCCTCCTCTTC
mm4_intron_Mrvi1_chr7_110963006 CAGTTCCCACCCCAGCTTCCCACTCCAGCCTCCCACCCCAGCCTCCCACCCCAGCCTCCCAGGGTTTGGCTTTTCAGATT
CCATACAACAGCTTTTAGACTTTCAACTCCCTCAAGTGCTGGCCTCTATCTCCTGA
mm4_intron_Dapp1_chr3_137977797 GGAGGAGTAGACCTAGCCGTTCCCAGAGCTGAGCCTCCCCTGCCTGGTTCCCTGGGCCTGCCCTGTTAGTCCTTACACCT
CCACAACAAATAGGGCTTTGTTTTTCTTCTCCTTTGAGGCCCCCTGATGCACTGAAGAGG
mm4_intergenic_n-R5s30|Rbfox1_chr16_5495572 AGGGTTCCAGGTTAGGGTCATCCCAGGGAGCCTGGAAGGGGAGGGTTATAAGAGCCACTCCTCAGGATGAAAGGGGC
mm4_intergenic_Pabpc6|Qk_chr17_9928012 ACCCTAAGCTGTTTCCCGTCTCTTTTGAAAGCTTCCCCAGCCAGCCAGCACCTCACTTGCAGGGCCCAGGGAGCCTTGTG
GGGGAGGA
GGACTGGGGGTGGGGGAGCCCCAGGAAGCTGGTGCGTCCTCCAGAGAGCAAA
mm4_intergenic_En1|B230209K01Rik_chr1_120942147 TGTGTGCAATGAATTCACAGAACAACTAACCTGAGAGCCTGGAGGGGGATGCTCGCCCACCAAAAATCTCACTGGAGTCC
CCCACTGTGCATCCTAACCCTCCAACAGAAGCCAG
mm4_exon_Pcbp1_chr6_86525964 TCACGTTGAGTCCGCTTTCAGTCACACCGGCGTCCATGGCGAGCGGCGGGCGGCGTTCGGGAGTTGGGCTCGTTACGTGG
TCAAGTCCTGGGCGGCTGGCGGGGGAGGGGAGGAGTAGGCCCGAACTG

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

Version 5.01 - Documentation  - Contact us - Downloads/local installation - Citation - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.