CRISPOR

DNA break repair outcome predictions

Guide: CCAAGTAAGCTCCTAATCCC AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGGGGGAGGGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGCTCCCTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCG`	(Wild-type sequence)
469	`GGGAAAACCCCCAAGTAAGCTCCTAATCCC--------TGGGATCTTGGCTGAGAAGAGG`	`8 bp deleted → frameshift`
447	`GGGAAAACCCCCAAGTAAGCTCC--------------CTGGGATCTTGGCTGAGAAGAGG`	`14 bp deleted → frameshift`
282	`GGGAAAACCCCCAAGTAAGCTCCTAATCCC-------CTGGGATCTTGGCTGAGAAGAGG`	`7 bp deleted → frameshift`
255	`GGGAAAACCCCCAAGTAAGCTCCTAATCC---------TGGGATCTTGGCTGAGAAGAGG`	`9 bp deleted → no frameshift`
235	`GGGAAAACCCCCAAGTAAGCTCCT---------------GGGATCTTGGCTGAGAAGAGG`	`15 bp deleted → no frameshift`
173	`GGGAAAACCCCCAAGTAAGCTCCT-----------CCCTGGGATCTTGGCTGAGAAGAGG`	`11 bp deleted → frameshift`
170	`GGGAAAACCCCCAAGTAAGCTCCTAATC-----------------TTGGCTGAGAAGAGG`	`17 bp deleted → frameshift`
163	`GGGAAAACCCCC--------------------------TGGGATCTTGGCTGAGAAGAGG`	`26 bp deleted → frameshift`
156	`GGGAAAACCCCCAAGTAAG-------------GCTCCCTGGGATCTTGGCTGAGAAGAGG`	`13 bp deleted → frameshift`
155	`GGGAAAACCCC---------------------------TGGGATCTTGGCTGAGAAGAGG`	`27 bp deleted → no frameshift`
148	`GGGAAAACCC----------------------------TGGGATCTTGGCTGAGAAGAGG`	`28 bp deleted → frameshift`
128	`GGGAAAACCCCCAAG-----------------GCTCCCTGGGATCTTGGCTGAGAAGAGG`	`17 bp deleted → frameshift`
122	`GGGAAAACCCCCAAGTAAGCT------------------GGGATCTTGGCTGAGAAGAGG`	`18 bp deleted → no frameshift`
114	`GGGAAAACCCCC-------------------------CTGGGATCTTGGCTGAGAAGAGG`	`25 bp deleted → frameshift`
111	`GGGAAAACCCCCAAGTAAGCT------------------------------GAGAAGAGG`	`30 bp deleted → no frameshift`
99	`GGGAAAACCCCCAAGTAAGCTCCT----------------------TGGCTGAGAAGAGG`	`22 bp deleted → frameshift`
99	`GGGA---------------------------------------TCTTGGCTGAGAAGAGG`	`39 bp deleted → no frameshift`
95	`GGGAAAACCCCCAAGTAAGCTC-----------------------TTGGCTGAGAAGAGG`	`23 bp deleted → frameshift`
94	`GGGAAAACC-----------------------------TGGGATCTTGGCTGAGAAGAGG`	`29 bp deleted → frameshift`
89	`GGG------------------------------CTCCCTGGGATCTTGGCTGAGAAGAGG`	`30 bp deleted → no frameshift`
86	`GGGAAAACCCCCAAGTAAGCT-------------------------TGGCTGAGAAGAGG`	`25 bp deleted → frameshift`
84	`GG-------------------------------CTCCCTGGGATCTTGGCTGAGAAGAGG`	`31 bp deleted → frameshift`
77	`GGGAAAACCCCCAAGTAAGCTCCT---------------------------GAGAAGAGG`	`27 bp deleted → no frameshift`
60	`GGGAAAACCCCCAAGTAAG--------------------------------------AGG`	`38 bp deleted → frameshift`
60	`GGG--------------------------------------GATCTTGGCTGAGAAGAGG`	`38 bp deleted → frameshift`
54	`GG----------------------------------------ATCTTGGCTGAGAAGAGG`	`40 bp deleted → frameshift`
52	`GGGAAAACCCCCAAGTAAG-----------------------------------AAGAGG`	`35 bp deleted → frameshift`
48	`GGGAAAACCCCCAAG------------------------------------------AGG`	`42 bp deleted → no frameshift`
44	`GGGAAAACCCCCAAGTAAGCTCCTAA------------------------------GAGG`	`30 bp deleted → no frameshift`
42	`GGGAAAACCCCCAAG---------------------------------------AAGAGG`	`39 bp deleted → no frameshift`
40	`GGGAAAACCCCCAAGTAAG----------------------------------------G`	`40 bp deleted → frameshift`
40	`GGG----------------------------------------------CTGAGAAGAGG`	`46 bp deleted → frameshift`
38	`GG-----------------------------------------------CTGAGAAGAGG`	`47 bp deleted → frameshift`
33	`GGGAAAACCCCCAAG--------------------------------------------G`	`44 bp deleted → frameshift`
31	`GGGAA---------------------------------------------------GAGG`	`51 bp deleted → no frameshift`
25	`GGGA-------------------------------------------------GAAGAGG`	`49 bp deleted → frameshift`
23	`GGG---------------------------------------------------------`	`57 bp deleted → no frameshift`
22	`GG----------------------------------------------------------`	`58 bp deleted → frameshift`
20	`GGGA------------------------------------------------------GG`	`54 bp deleted → no frameshift`
17	`GGGAAAA-------------------------------------------------GAGG`	`49 bp deleted → frameshift`
16	`GGGAAA--------------------------------------------------GAGG`	`50 bp deleted → frameshift`

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