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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| AGCTCCTAATCCCAGGCTCCCTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAGGCGCCGGGTAGCCCCAGC | (Wild-type sequence) | |
| 335 | CCCAGGCTCCCTGGGATCTTGGCTGAG--------CTAAGGCGACAAGGCAGGCGCCGGG | 8 bp deleted → frameshift |
| 271 | CCCAGGCTCCCTGGGATCTTGGCTGAGAAG--GAGCTAAGGCGACAAGGCAGGCGCCGGG | 2 bp deleted → frameshift |
| 261 | CCCAGGCTCCCTGGGATCTTGGCT-------------AAGGCGACAAGGCAGGCGCCGGG | 13 bp deleted → frameshift |
| 242 | CCCAGGCTCCCTGGGATCTTGGCTGAGAAG----------GCGACAAGGCAGGCGCCGGG | 10 bp deleted → frameshift |
| 233 | CCCAGGCTCCCTGGGATCTTGGCTGAGAAG-----CTAAGGCGACAAGGCAGGCGCCGGG | 5 bp deleted → frameshift |
| 233 | CCCAGGCTCCCTGGGATCTTGGCTGAG-----GAGCTAAGGCGACAAGGCAGGCGCCGGG | 5 bp deleted → frameshift |
| 232 | CCCAGGCTCCCTGGGATCTTGGC-------------------GACAAGGCAGGCGCCGGG | 19 bp deleted → frameshift |
| 230 | CCCAGGCTCCCTGGGATCTTGG-----------AGCTAAGGCGACAAGGCAGGCGCCGGG | 11 bp deleted → frameshift |
| 222 | CCCAGGCTCCCTGGGATCTTGGCTGAGA------GCTAAGGCGACAAGGCAGGCGCCGGG | 6 bp deleted → no frameshift |
| 203 | CCCAGGCTCCCTGGGA------------------GCTAAGGCGACAAGGCAGGCGCCGGG | 18 bp deleted → no frameshift |
| 162 | CCCAGGCTCCCTGGGATCTTGGCTGAGAAG------------------GCAGGCGCCGGG | 18 bp deleted → no frameshift |
| 156 | CCCAGGCTCCCTGGGATCTTGGCTGAG-------------GCGACAAGGCAGGCGCCGGG | 13 bp deleted → frameshift |
| 155 | CCCAGGCTCCCTGGGATCTTGGC---------------------------AGGCGCCGGG | 27 bp deleted → no frameshift |
| 154 | CCCAGGCTCCCTGG-------------------AGCTAAGGCGACAAGGCAGGCGCCGGG | 19 bp deleted → frameshift |
| 134 | CCCAGGCTCCCTGGGATCTTGGCTGAGA----------------CAAGGCAGGCGCCGGG | 16 bp deleted → frameshift |
| 129 | CCCAGG---------------------------AGCTAAGGCGACAAGGCAGGCGCCGGG | 27 bp deleted → no frameshift |
| 127 | CCCAGGCTCCCTGGGATCTTGGC-------------------------------GCCGGG | 31 bp deleted → frameshift |
| 122 | CCCAGGCTCCCTGGGATCTTGGCTGA------------------CAAGGCAGGCGCCGGG | 18 bp deleted → no frameshift |
| 122 | CCCAGGCTCCCTGGGATCT------------------AAGGCGACAAGGCAGGCGCCGGG | 18 bp deleted → no frameshift |
| 121 | CCCAGGC-----------------------------------GACAAGGCAGGCGCCGGG | 35 bp deleted → frameshift |
| 117 | CCCAGGCT-----------------------------AAGGCGACAAGGCAGGCGCCGGG | 29 bp deleted → frameshift |
| 109 | CCCAGGCTCCCTGGG--------------------------CGACAAGGCAGGCGCCGGG | 26 bp deleted → frameshift |
| 105 | CCCAGGCTCCCTGGGATCTTGGCTGAG---------------------GCAGGCGCCGGG | 21 bp deleted → no frameshift |
| 103 | CCCAGGCTCCCTGG---------------------------CGACAAGGCAGGCGCCGGG | 27 bp deleted → no frameshift |
| 99 | CCCAGGCTCCCTGGGATCTTGGCTGAGAAG----------------------GCGCCGGG | 22 bp deleted → frameshift |
| 86 | CCCAGGCTCCCTGGGATCTTGGCTGAG-------------------------GCGCCGGG | 25 bp deleted → frameshift |
| 86 | CCCAGGCTCCCT-------------------------AAGGCGACAAGGCAGGCGCCGGG | 25 bp deleted → frameshift |
| 85 | CCCAGGC-----------------------------------------------GCCGGG | 47 bp deleted → frameshift |
| 81 | CCCAGGC-------------------------------------------AGGCGCCGGG | 43 bp deleted → frameshift |
| 76 | CCCAGGCTCCCTGGGATCTTGGC---------------------------------CGGG | 33 bp deleted → no frameshift |
| 74 | CCCAGGCTCCCTGGGA----------------------------CAAGGCAGGCGCCGGG | 28 bp deleted → frameshift |
| 73 | CCCAGGCTCCCTGGG----------------------------------CAGGCGCCGGG | 34 bp deleted → frameshift |
| 69 | CCCAGGCTCCCTGG-----------------------------------CAGGCGCCGGG | 35 bp deleted → frameshift |
| 66 | CCCAG------------------------------CTAAGGCGACAAGGCAGGCGCCGGG | 30 bp deleted → no frameshift |
| 63 | CCCAGGCTCCCTGGG--------------------------------------------- | 45 bp deleted → no frameshift |
| 62 | CCCAGGCTCCCTGGGATCTTGG-------------------------------------G | 37 bp deleted → frameshift |
| 60 | CCCAGGCTCCCTGGGATCTTGG-------------------------------------- | 38 bp deleted → frameshift |
| 60 | CCCAGGCTCCCTGGG--------------------------------------CGCCGGG | 38 bp deleted → frameshift |
| 56 | CCCAGGCTCCCTGG---------------------------------------CGCCGGG | 39 bp deleted → no frameshift |
| 44 | CCCAGGCTCCCTGGG--------------------------------------------G | 44 bp deleted → frameshift |
| 40 | CCCAGGCTCCCTGG---------------------------------------------- | 46 bp deleted → frameshift |
| 40 | CCCAGGCTCCC----------------------------------------------GGG | 46 bp deleted → frameshift |
| 38 | CCCAGGCTCC-----------------------------------------------GGG | 47 bp deleted → frameshift |
| 36 | CCCA------------------------------------------AGGCAGGCGCCGGG | 42 bp deleted → no frameshift |
| 34 | CCCAGGC-------------------------------------------------CGGG | 49 bp deleted → frameshift |
| 28 | CCCAGG-----------------------------------------------------G | 53 bp deleted → frameshift |
| 26 | CCCAGG------------------------------------------------------ | 54 bp deleted → no frameshift |
| 26 | CCC------------------------------------------------------GGG | 54 bp deleted → no frameshift |
| 25 | CC-------------------------------------------------------GGG | 55 bp deleted → frameshift |