CRISPOR

DNA break repair outcome predictions

Guide: TGTGCCGCACCGCCTCTGGG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTGTGATGCGAGCGCTCCTCTGTGCCGCACCGCCTCTGGGAGGAAGCCCCATTGCCCTCTTCTTTCTAAGCTGTCATCCT`	(Wild-type sequence)
344	`AGCGCTCCTCTGTGCCGCACCGCCTCTGGG---AAGCCCCATTGCCCTCTTCTTTCTAAG`	`3 bp deleted → no frameshift`
327	`AGCGCTCCTCTGTGCCGCACCGCCTCTGG----AAGCCCCATTGCCCTCTTCTTTCTAAG`	`4 bp deleted → frameshift`
298	`AGCGCTCCTCTGTGCCGCACCGCC--------------CCATTGCCCTCTTCTTTCTAAG`	`14 bp deleted → frameshift`
253	`AGCGCTCCTCTGTGCCGCACCGCCTCT-----------------------TCTTTCTAAG`	`23 bp deleted → frameshift`
199	`AGCGCTCCTCTGTGCCGCACCGCC----------------------CTCTTCTTTCTAAG`	`22 bp deleted → frameshift`
199	`AGCGCTCCTCTGTGCC----------------------CCATTGCCCTCTTCTTTCTAAG`	`22 bp deleted → frameshift`
188	`AGCGCTCCTCTGTGCCGCACCGCC---------------CATTGCCCTCTTCTTTCTAAG`	`15 bp deleted → no frameshift`
179	`AGCGCTCCTCTGTGCCGCACCGCC----------------ATTGCCCTCTTCTTTCTAAG`	`16 bp deleted → frameshift`
170	`AGCGCTCCTCTGTGCCGCACC-----------------CCATTGCCCTCTTCTTTCTAAG`	`17 bp deleted → frameshift`
162	`AGCGCTCCTCTGTGCCGCACC------------------CATTGCCCTCTTCTTTCTAAG`	`18 bp deleted → no frameshift`
156	`AGCGCTCCTCTGTGCC------------------------------CTCTTCTTTCTAAG`	`30 bp deleted → no frameshift`
154	`AGCGCTCCTCTGTGCCGCACC-------------------ATTGCCCTCTTCTTTCTAAG`	`19 bp deleted → frameshift`
154	`AGCGCTCCTCTGTGCCGC-------------------CCCATTGCCCTCTTCTTTCTAAG`	`19 bp deleted → frameshift`
134	`AGCGCTCCTCTGTGCCGCACCGCCTCTG----------------CCCTCTTCTTTCTAAG`	`16 bp deleted → frameshift`
126	`AGCGCTCCTCTGTGCC-----------------------CATTGCCCTCTTCTTTCTAAG`	`23 bp deleted → frameshift`
120	`AGCGCTCCTCTGTGCC------------------------ATTGCCCTCTTCTTTCTAAG`	`24 bp deleted → no frameshift`
114	`AGCGCTCCTCTGTGCCGCACC-------------------------CTCTTCTTTCTAAG`	`25 bp deleted → frameshift`
113	`AGCGCTCCTCT---------------------------------------TCTTTCTAAG`	`39 bp deleted → no frameshift`
109	`AGCGCTCCTCTGTGCCGCACCGCCTCT--------------------------TTCTAAG`	`26 bp deleted → frameshift`
109	`AGCGCTCCTCTGTGCCGCACC--------------------------TCTTCTTTCTAAG`	`26 bp deleted → frameshift`
105	`AGCGCTCCTCTGTGCCGCACCGCCTCT---------------------CTTCTTTCTAAG`	`21 bp deleted → no frameshift`
103	`AGCGCTCCTCTGTGCCGC---------------------------CCTCTTCTTTCTAAG`	`27 bp deleted → no frameshift`
99	`AGCGCTCCTCTGTGCCGCA----------------------TTGCCCTCTTCTTTCTAAG`	`22 bp deleted → frameshift`
91	`AGC----------------------------------CCCATTGCCCTCTTCTTTCTAAG`	`34 bp deleted → frameshift`
89	`AGCGCTCCTCTGTGCCGCACCGCCTCT------------------------------AAG`	`30 bp deleted → no frameshift`
89	`AGCGCTCC------------------------------CCATTGCCCTCTTCTTTCTAAG`	`30 bp deleted → no frameshift`
86	`AGCGCTCCTCTGTGCCGCACCGCCT-------------------------TCTTTCTAAG`	`25 bp deleted → frameshift`
84	`AGCGCTCCTCTGTGCC-------------------------------TCTTCTTTCTAAG`	`31 bp deleted → frameshift`
84	`AGCGCTCC-------------------------------CATTGCCCTCTTCTTTCTAAG`	`31 bp deleted → frameshift`
80	`AGCGCTCC--------------------------------ATTGCCCTCTTCTTTCTAAG`	`32 bp deleted → frameshift`
80	`AGCGC--------------------------------CCCATTGCCCTCTTCTTTCTAAG`	`32 bp deleted → frameshift`
74	`AGCGCTCCTCTGTGCCGCACCGCCT----------------------------TTCTAAG`	`28 bp deleted → frameshift`
66	`AG------------------------------GAAGCCCCATTGCCCTCTTCTTTCTAAG`	`30 bp deleted → no frameshift`
61	`AGCGCTC------------------------------------------TTCTTTCTAAG`	`42 bp deleted → no frameshift`
60	`AGCGCTCCTCTGTGCCGCACCGCCT--------------------------------AAG`	`32 bp deleted → frameshift`
60	`AGCGCTCCTCTG--------------------------------CCCTCTTCTTTCTAAG`	`32 bp deleted → frameshift`
60	`AGCGCTCC--------------------------------------CTCTTCTTTCTAAG`	`38 bp deleted → frameshift`
54	`AGCGC----------------------------------------CCTCTTCTTTCTAAG`	`40 bp deleted → frameshift`
48	`AGCGCTCCTCT------------------------------------------TTCTAAG`	`42 bp deleted → no frameshift`
48	`AGC------------------------------------------CCTCTTCTTTCTAAG`	`42 bp deleted → no frameshift`
47	`AGCGCTCCTCT-------------------------------------CTTCTTTCTAAG`	`37 bp deleted → frameshift`
40	`AGCGCTCCTCT----------------------------------------------AAG`	`46 bp deleted → frameshift`
38	`AGCGCTCCT-----------------------------------------TCTTTCTAAG`	`41 bp deleted → frameshift`
33	`AGCGCTCCT--------------------------------------------TTCTAAG`	`44 bp deleted → frameshift`
33	`AGCGCT--------------------------------------------TCTTTCTAAG`	`44 bp deleted → frameshift`
31	`AGCGCTC---------------------------------------------TTTCTAAG`	`45 bp deleted → no frameshift`
28	`AGCGCT-----------------------------------------------TTCTAAG`	`47 bp deleted → frameshift`
27	`AGCGCTCCT------------------------------------------------AAG`	`48 bp deleted → no frameshift`
25	`AGCGCTC-------------------------------------------------TAAG`	`49 bp deleted → frameshift`
23	`AGCGCT---------------------------------------------------AAG`	`51 bp deleted → no frameshift`
16	`AG----------------------------------------------------------`	`58 bp deleted → frameshift`

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