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Contents:

PCR primers for off-targets of GGACTCTCCAGAGGGGGGGA GGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313817_F TCGTCGGCAGCGTCAGGGAGCCTGGGATTAGGAG 60.1 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313817_R GTCTCGTGGGCTCGGGTCCTTTCGGCTCTCCATCC 60.1 1.00
mm4_intron_Txlna_chr4_129637043_F TCGTCGGCAGCGTCATGTGTTCCTGCACCCTAGC 60.0 0.92
mm4_intron_Txlna_chr4_129637043_R GTCTCGTGGGCTCGGTGGTCAGTCCATGCCCTTTC 59.9 0.92
mm4_intron_Mctp2_chr7_72203190_F Primer3: not found at this Tm N.d. 0.76
mm4_intron_Mctp2_chr7_72203190_R Primer3: not found at this Tm N.d. 0.76
mm3_intergenic_E030044B06Rik|mmu-mir-8092/Zfp518a_chr19_40874235_F Primer3: not found at this Tm N.d. 0.67
mm3_intergenic_E030044B06Rik|mmu-mir-8092/Zfp518a_chr19_40874235_R Primer3: not found at this Tm N.d. 0.67
mm4_intron_Ppp2r5e_chr12_75512004_F Primer3: not found at this Tm N.d. 0.65
mm4_intron_Ppp2r5e_chr12_75512004_R Primer3: not found at this Tm N.d. 0.65
mm3_intergenic_Gm13418|Gm13417_chr2_24513082_F TCGTCGGCAGCGTCATGCTGGCAGGAAAGAACCA 59.8 0.64
mm3_intergenic_Gm13418|Gm13417_chr2_24513082_R GTCTCGTGGGCTCGGTCTGCATGGGCTCATTTCCC 60.3 0.64
mm4_exon_Igf2bp1/Mir3063_chr11_95961324_F Primer3: not found at this Tm N.d. 0.57
mm4_exon_Igf2bp1/Mir3063_chr11_95961324_R Primer3: not found at this Tm N.d. 0.57
mm4_intergenic_Tmem117|Nell2_chr15_95209423_F TCGTCGGCAGCGTCGGCCCAGAGGATAGCAAAGG 60.1 0.55
mm4_intergenic_Tmem117|Nell2_chr15_95209423_R GTCTCGTGGGCTCGGTGTCAAAACAGGAGCTCCCC 59.8 0.55
mm4_intergenic_Rras2|Copb1_chr7_114169156_F TCGTCGGCAGCGTCGGGGCAGATTTCACCCTCTC 60.1 0.54
mm4_intergenic_Rras2|Copb1_chr7_114169156_R GTCTCGTGGGCTCGGGTGAGGAACAGTAGGCCAGG 59.7 0.54
mm4_intron_Adamtsl1_chr4_86388980_F TCGTCGGCAGCGTCATAGTGCTTGCAGGCCAGAG 60.1 0.54
mm4_intron_Adamtsl1_chr4_86388980_R GTCTCGTGGGCTCGGGCTTGGTCCTCAGTGTTCGA 59.9 0.54
mm4_intergenic_Gm24291|Mon1b_chr8_113551663_F TCGTCGGCAGCGTCGCGTTGTTCTGAACCGGAAG 59.7 0.54
mm4_intergenic_Gm24291|Mon1b_chr8_113551663_R GTCTCGTGGGCTCGGCCTCTCCTTCCTCCCTTGGA 59.9 0.54
mm3_intergenic_Gm22251|Zfp521_chr18_13431592_F Primer3: not found at this Tm N.d. 0.54
mm3_intergenic_Gm22251|Zfp521_chr18_13431592_R Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Gm4487|Gm24073_chr14_113620140_F Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Gm4487|Gm24073_chr14_113620140_R Primer3: not found at this Tm N.d. 0.54
mm4_intron_Eif3m_chr2_105015043_F TCGTCGGCAGCGTCATCTGCCCTGCTTCCAAAGT 59.5 0.52
mm4_intron_Eif3m_chr2_105015043_R GTCTCGTGGGCTCGGTGGATACAGGTGGCAGGAAAC 59.9 0.52
mm4_intron_Fam129c_chr8_71602993_F TCGTCGGCAGCGTCACAGGTGAGAGGTGGAGCTA 59.5 0.51
mm4_intron_Fam129c_chr8_71602993_R GTCTCGTGGGCTCGGATAAACATGTCCCTCGGCCC 59.8 0.51
mm4_intron_Myo18b_chr5_112787221_F TCGTCGGCAGCGTCCACGGGTCTGTGGTCTTTGA 59.8 0.51
mm4_intron_Myo18b_chr5_112787221_R GTCTCGTGGGCTCGGGGACTAAGGGAGGTCGGGAA 60.3 0.51
mm4_exon_Ctnnd1_chr2_84650573_F Primer3: not found at this Tm N.d. 0.50
mm4_exon_Ctnnd1_chr2_84650573_R Primer3: not found at this Tm N.d. 0.50
mm4_intergenic_4930596D02Rik|Gm7853_chr14_36058077_F Primer3: not found at this Tm N.d. 0.50
mm4_intergenic_4930596D02Rik|Gm7853_chr14_36058077_R Primer3: not found at this Tm N.d. 0.50
mm4_intron_Sntb1_chr15_55785609_F TCGTCGGCAGCGTCAGCCTCAGAGAACCAGGACT 59.8 0.48
mm4_intron_Sntb1_chr15_55785609_R GTCTCGTGGGCTCGGAGTTCACTGTCCTTTCATGACT 57.3 0.48
mm4_intergenic_Nsg2|Gm12108_chr11_32066875_F Primer3: not found at this Tm N.d. 0.47
mm4_intergenic_Nsg2|Gm12108_chr11_32066875_R Primer3: not found at this Tm N.d. 0.47
mm2_intron_Ppa1_chr10_61653062_F Primer3: not found at this Tm N.d. 0.46
mm2_intron_Ppa1_chr10_61653062_R Primer3: not found at this Tm N.d. 0.46
mm2_intron_Ccdc30_chr4_119385354_F TCGTCGGCAGCGTCTGCAAATCGCCAATAGTCGC 59.6 0.45
mm2_intron_Ccdc30_chr4_119385354_R GTCTCGTGGGCTCGGAGTCTTTCCCTCCCTCACCG 60.9 0.45
mm3_intergenic_Gm13750|Gm25748_chr1_64174880_F Primer3: not found at this Tm N.d. 0.44
mm3_intergenic_Gm13750|Gm25748_chr1_64174880_R Primer3: not found at this Tm N.d. 0.44
mm4_intron_Megf11_chr9_64478061_F Primer3: not found at this Tm N.d. 0.44
mm4_intron_Megf11_chr9_64478061_R Primer3: not found at this Tm N.d. 0.44
mm3_intergenic_Ercc4|Mkl2_chr16_13208210_F Primer3: not found at this Tm N.d. 0.44
mm3_intergenic_Ercc4|Mkl2_chr16_13208210_R Primer3: not found at this Tm N.d. 0.44
mm4_intergenic_Ninj2|Gm25327_chr6_120111952_F Primer3: not found at this Tm N.d. 0.43
mm4_intergenic_Ninj2|Gm25327_chr6_120111952_R Primer3: not found at this Tm N.d. 0.43
mm4_intron_Epha5_chr5_84068676_F Primer3: not found at this Tm N.d. 0.43
mm4_intron_Epha5_chr5_84068676_R Primer3: not found at this Tm N.d. 0.43
mm4_intron_Epb4.1l1_chr2_156509372_F TCGTCGGCAGCGTCGATGGAGGTATGGCTCAGGC 59.9 0.42
mm4_intron_Epb4.1l1_chr2_156509372_R GTCTCGTGGGCTCGGTACCGGCTGTCTCTTCTGGA 59.9 0.42
mm4_intergenic_Glra3|Hpgd_chr8_56158478_F Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Glra3|Hpgd_chr8_56158478_R Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Fuca1|Hmgcl_chr4_135940555_F Primer3: not found at this Tm N.d. 0.42
mm4_intergenic_Fuca1|Hmgcl_chr4_135940555_R Primer3: not found at this Tm N.d. 0.42
mm4_intron_Tpd52_chr3_8949197_F TCGTCGGCAGCGTCTGCTGTTTAACAATGGAGAGAAGA 58.3 0.42
mm4_intron_Tpd52_chr3_8949197_R GTCTCGTGGGCTCGGCAGTCTCAGTGCTGTGCTGA 59.9 0.42
mm4_intergenic_Tas1r2|Pax7_chr4_139710612_F TCGTCGGCAGCGTCAAGGCAACAAAGGACCCCAA 60.0 0.42
mm4_intergenic_Tas1r2|Pax7_chr4_139710612_R GTCTCGTGGGCTCGGCTGTCACCCATTACCCACCC 60.0 0.42
mm4_intron_Creb3l2_chr6_37337154_F Primer3: not found at this Tm N.d. 0.41
mm4_intron_Creb3l2_chr6_37337154_R Primer3: not found at this Tm N.d. 0.41
mm4_intron_A430078G23Rik_chr8_3361834_F Primer3: not found at this Tm N.d. 0.41
mm4_intron_A430078G23Rik_chr8_3361834_R Primer3: not found at this Tm N.d. 0.41
mm4_intergenic_Dock5|Nefm/Nefl_chr14_68021621_F Primer3: not found at this Tm N.d. 0.41
mm4_intergenic_Dock5|Nefm/Nefl_chr14_68021621_R Primer3: not found at this Tm N.d. 0.41
mm4_intron_Slc22a5_chr11_53887776_F TCGTCGGCAGCGTCTGCCATGAAACTGCAAAGGC 59.9 0.40
mm4_intron_Slc22a5_chr11_53887776_R GTCTCGTGGGCTCGGCCTGCCTGTGCTAGCCAT 59.4 0.40
mm4_intergenic_Dysf|Cyp26b1_chr6_84360426_F TCGTCGGCAGCGTCCCAACACAGGGGTCTTCAGG 60.2 0.40
mm4_intergenic_Dysf|Cyp26b1_chr6_84360426_R GTCTCGTGGGCTCGGTGCTTTTCCTCCCATCCCAG 59.6 0.40
mm4_intergenic_Nrg3|Gm10713_chr14_39648420_F Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Nrg3|Gm10713_chr14_39648420_R Primer3: not found at this Tm N.d. 0.39
mm4_exon_Iqgap3_chr3_88107521_F TCGTCGGCAGCGTCTTGCCTGACCAATGTCTCCC 59.9 0.38
mm4_exon_Iqgap3_chr3_88107521_R GTCTCGTGGGCTCGGCTGGTTCAAGAGGTGAGCGA 59.6 0.38
mm4_intron_Snd1_chr6_28900999_F TCGTCGGCAGCGTCAAAGGCCAAGGAGATGAGGC 60.0 0.38
mm4_intron_Snd1_chr6_28900999_R GTCTCGTGGGCTCGGAAGTCCTGGTGTGTGTGTGA 59.0 0.38
mm4_intergenic_0610040F04Rik|Gm17055_chr6_108622799_F Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_0610040F04Rik|Gm17055_chr6_108622799_R Primer3: not found at this Tm N.d. 0.37
mm4_intron_Fhod3_chr18_24846851_F Primer3: not found at this Tm N.d. 0.37
mm4_intron_Fhod3_chr18_24846851_R Primer3: not found at this Tm N.d. 0.37
mm4_intron_Dctn2_chr10_127273546_F Primer3: not found at this Tm N.d. 0.37
mm4_intron_Dctn2_chr10_127273546_R Primer3: not found at this Tm N.d. 0.37
mm4_intergenic_Rps16|Plekhg2_chr7_28357189_F TCGTCGGCAGCGTCCCTCCTTTGAATGCCGGGAA 60.3 0.36
mm4_intergenic_Rps16|Plekhg2_chr7_28357189_R GTCTCGTGGGCTCGGCCCAAGGTCAGAACTGAGGC 60.3 0.36
mm4_exon_Pnkd_chr1_74286137_F TCGTCGGCAGCGTCTCTGAAATGCGGGTGGAGAG 59.7 0.36
mm4_exon_Pnkd_chr1_74286137_R GTCTCGTGGGCTCGGTGCTCCACAGAAAAGCCACT 59.8 0.36
mm4_intron_Sipa1l3_chr7_29444663_F TCGTCGGCAGCGTCTGGGTGACTCAGAGGAACCT 59.8 0.36
mm4_intron_Sipa1l3_chr7_29444663_R GTCTCGTGGGCTCGGTTCCATGGGTGATTGCTGCT 59.9 0.36
mm4_intron_Sufu_chr19_46447211_F Primer3: not found at this Tm N.d. 0.36
mm4_intron_Sufu_chr19_46447211_R Primer3: not found at this Tm N.d. 0.36
mm4_intron_Ap3b1_chr13_94372442_F TCGTCGGCAGCGTCAGAGGAGGGCAGGATGGATT 60.0 0.36
mm4_intron_Ap3b1_chr13_94372442_R GTCTCGTGGGCTCGGTAGCTGCAGTCAGACAAGCC 60.0 0.36
mm4_intergenic_Anapc15-ps|4732465J04Rik_chr10_95763497_F TCGTCGGCAGCGTCTGTTTGTGTACACCCGGAGC 60.5 0.36
mm4_intergenic_Anapc15-ps|4732465J04Rik_chr10_95763497_R GTCTCGTGGGCTCGGGTCAGGCTGTATCCGGCTTT 60.1 0.36
mm4_intron_Arhgap10_chr8_77276893_F Primer3: not found at this Tm N.d. 0.36
mm4_intron_Arhgap10_chr8_77276893_R Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_Gm9081|Avpr1a_chr10_122344668_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Gm9081|Avpr1a_chr10_122344668_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Gm24728|Lmo4_chr3_144155556_F Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Gm24728|Lmo4_chr3_144155556_R Primer3: not found at this Tm N.d. 0.35
mm3_intergenic_Gjb2|Gm4491_chr14_57106695_F Primer3: not found at this Tm N.d. 0.35
mm3_intergenic_Gjb2|Gm4491_chr14_57106695_R Primer3: not found at this Tm N.d. 0.35
mm4_intron_Fut9_chr4_25787435_F Primer3: not found at this Tm N.d. 0.35
mm4_intron_Fut9_chr4_25787435_R Primer3: not found at this Tm N.d. 0.35
mm4_intergenic_Kif26b|Smyd3_chr1_178938162_F Primer3: not found at this Tm N.d. 0.34
mm4_intergenic_Kif26b|Smyd3_chr1_178938162_R Primer3: not found at this Tm N.d. 0.34
mm3_intergenic_Agfg1|Slc19a3_chr1_82911556_F Primer3: not found at this Tm N.d. 0.34
mm3_intergenic_Agfg1|Slc19a3_chr1_82911556_R Primer3: not found at this Tm N.d. 0.34
mm4_intron_2310035C23Rik_chr1_105734810_F Primer3: not found at this Tm N.d. 0.33
mm4_intron_2310035C23Rik_chr1_105734810_R Primer3: not found at this Tm N.d. 0.33
mm4_intergenic_Ppt1/Cap1|Cap1_chr4_122861471_F TCGTCGGCAGCGTCGACAACAGCCTGATTTGGGG 59.1 0.32
mm4_intergenic_Ppt1/Cap1|Cap1_chr4_122861471_R GTCTCGTGGGCTCGGAGAAGCCAGGTAGTCCATGA 57.7 0.32
mm4_intergenic_Mcf2|Atp11c_chrX_60211242_F Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Mcf2|Atp11c_chrX_60211242_R Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Atp1a3|Grik5_chr7_25008252_F TCGTCGGCAGCGTCGTGCATAACGCTCCCAATGC 60.2 0.32
mm4_intergenic_Atp1a3|Grik5_chr7_25008252_R GTCTCGTGGGCTCGGTCCTCCATCCCTGTGACCTT 59.8 0.32
mm4_intergenic_Vmn1r-ps8|Vmn1r13_chr6_57205789_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Vmn1r-ps8|Vmn1r13_chr6_57205789_R Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Fbxl17|4930405O22Rik_chr17_63245525_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Fbxl17|4930405O22Rik_chr17_63245525_R Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Thumpd2|Slc8a1_chr17_81178733_F TCGTCGGCAGCGTCGGAGGGCCCAAATGCTTAGT 60.0 0.31
mm4_intergenic_Thumpd2|Slc8a1_chr17_81178733_R GTCTCGTGGGCTCGGCCCACACATGCCCAACACTA 60.2 0.31
mm4_intergenic_Wnt11/Gm8398|Uvrag_chr7_98867133_F TCGTCGGCAGCGTCACAGTGGCTGGAAGGATTGG 59.9 0.30
mm4_intergenic_Wnt11/Gm8398|Uvrag_chr7_98867133_R GTCTCGTGGGCTCGGCCTGGTAGGCTCTGGTGTTC 59.7 0.30
mm4_intergenic_Gm833|Gm25779_chr4_152903397_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm833|Gm25779_chr4_152903397_R Primer3: not found at this Tm N.d. 0.30
mm3_intron_Shfm1_chr6_6572277_F TCGTCGGCAGCGTCTGTAGCTATACATTTCCTGTGTCT 57.2 0.30
mm3_intron_Shfm1_chr6_6572277_R GTCTCGTGGGCTCGGCCGCACCTTCATGACCTCAT 60.1 0.30
mm4_intergenic_Vegfa|Mrps18a_chr17_46110181_F TCGTCGGCAGCGTCAGAGGTGCCAATGTGGGAAA 59.5 0.29
mm4_intergenic_Vegfa|Mrps18a_chr17_46110181_R GTCTCGTGGGCTCGGCACCCTAGTCACCCTTGTGG 59.6 0.29
mm4_intergenic_Clec16a|Gm15558_chr16_10655586_F TCGTCGGCAGCGTCCTGCAGTAGTCCCTGTCCCT 60.6 0.29
mm4_intergenic_Clec16a|Gm15558_chr16_10655586_R GTCTCGTGGGCTCGGAGAAAGCCATGCTGCAGCTA 60.0 0.29
mm4_intron_Zfp787_chr7_6150785_F Primer3: not found at this Tm N.d. 0.29
mm4_intron_Zfp787_chr7_6150785_R Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_U7|Gm11987_chr11_7970789_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_U7|Gm11987_chr11_7970789_R Primer3: not found at this Tm N.d. 0.29
mm3_intergenic_Gm22251|Zfp521_chr18_13461464_F TCGTCGGCAGCGTCTGTCTCACCCTTCACTCTTCT 58.0 0.29
mm3_intergenic_Gm22251|Zfp521_chr18_13461464_R GTCTCGTGGGCTCGGGATCACGCAGAGGCACAAAC 59.8 0.29
mm3_intron_Llgl2_chr11_115855263_F TCGTCGGCAGCGTCGGCTCAGCAATGGAGAAGGT 60.0 0.28
mm3_intron_Llgl2_chr11_115855263_R GTCTCGTGGGCTCGGGGAGGGGTGGCAGTTCTTTT 60.1 0.28
mm4_intergenic_Gm14285|4930425F17Rik_chr2_131642036_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm14285|4930425F17Rik_chr2_131642036_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm5909|Gypa_chr8_80149961_F TCGTCGGCAGCGTCCCCCAGGGCCATCTTTCTTT 59.9 0.28
mm4_intergenic_Gm5909|Gypa_chr8_80149961_R GTCTCGTGGGCTCGGAAGCAGCCAAAAGGACAAAAT 57.1 0.28
mm4_intron_Ctdspl_chr9_119010667_F Primer3: not found at this Tm N.d. 0.28
mm4_intron_Ctdspl_chr9_119010667_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Gm12360/Pcsk2|Pcsk2_chr2_143798216_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm12360/Pcsk2|Pcsk2_chr2_143798216_R Primer3: not found at this Tm N.d. 0.27
mm4_intron_Capn13_chr17_73356143_F TCGTCGGCAGCGTCTGTGAGTTGCCTGGTGTCTT 59.4 0.27
mm4_intron_Capn13_chr17_73356143_R GTCTCGTGGGCTCGGCAGAGGGACAGAGGGGTGTA 59.9 0.27
mm4_intron_Nfx1_chr4_40990378_F Primer3: not found at this Tm N.d. 0.27
mm4_intron_Nfx1_chr4_40990378_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm6317|Gm25383_chr13_78402196_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Gm6317|Gm25383_chr13_78402196_R Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Dmrta2|Elavl4_chr4_110018250_F Primer3: not found at this Tm N.d. 0.27
mm4_intergenic_Dmrta2|Elavl4_chr4_110018250_R Primer3: not found at this Tm N.d. 0.27
mm4_intron_Ano10_chr9_122265210_F Primer3: not found at this Tm N.d. 0.27
mm4_intron_Ano10_chr9_122265210_R Primer3: not found at this Tm N.d. 0.27
mm4_intron_B230323A14Rik_chr9_69767712_F TCGTCGGCAGCGTCCTTGGCACAGAAGCTGCAAG 60.0 0.26
mm4_intron_B230323A14Rik_chr9_69767712_R GTCTCGTGGGCTCGGGATCCCAACTGTGACCCACC 60.3 0.26
mm4_intergenic_Aqp6|Racgap1_chr15_99607471_F TCGTCGGCAGCGTCGTGCTTTGAGGGACGTCTGG 60.9 0.26
mm4_intergenic_Aqp6|Racgap1_chr15_99607471_R GTCTCGTGGGCTCGGGCTCTCCGGCTTTAACCTGA 59.7 0.26
mm4_intergenic_Gm25130|Gm22970_chr14_99492800_F TCGTCGGCAGCGTCTCCAGAGATTCATGTAGCAGCA 59.2 0.25
mm4_intergenic_Gm25130|Gm22970_chr14_99492800_R GTCTCGTGGGCTCGGCCCCAAGGGAAGAGTTCACTA 58.7 0.25
mm4_intergenic_Gm24861|Chodl_chr16_78915773_F Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Gm24861|Chodl_chr16_78915773_R Primer3: not found at this Tm N.d. 0.25
mm4_intron_Sptlc1_chr13_53344662_F TCGTCGGCAGCGTCACACAAACCTAGCTGCACCT 59.5 0.25
mm4_intron_Sptlc1_chr13_53344662_R GTCTCGTGGGCTCGGTGTCTGGGTGGATCTGGTCA 60.1 0.25
mm4_exon_Bpifb1_chr2_154213446_F Primer3: not found at this Tm N.d. 0.25
mm4_exon_Bpifb1_chr2_154213446_R Primer3: not found at this Tm N.d. 0.25
mm4_intron_Apol7d_chr1_71655060_F TCGTCGGCAGCGTCAGGGCTCCTTGTAAAACAGTGA 59.5 0.24
mm4_intron_Apol7d_chr1_71655060_R GTCTCGTGGGCTCGGAAAGCCTTCCCTTCGGGTTT 59.8 0.24
mm4_intergenic_Pard3|Gm6921_chr8_127518633_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Pard3|Gm6921_chr8_127518633_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gata3|Gm13262_chr2_9896304_F TCGTCGGCAGCGTCCGGGGCTGGAAATTTGTGTG 60.0 0.24
mm4_intergenic_Gata3|Gm13262_chr2_9896304_R GTCTCGTGGGCTCGGATCCACCCATCCATCCCTGA 60.0 0.24
mm4_intergenic_Zfp746|Gm16630_chr6_48115082_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Zfp746|Gm16630_chr6_48115082_R Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm26256|n-R5s61_chr12_68358056_F Primer3: not found at this Tm N.d. 0.24
mm4_intergenic_Gm26256|n-R5s61_chr12_68358056_R Primer3: not found at this Tm N.d. 0.24
mm3_exon_Nodal_chr10_61418206_F TCGTCGGCAGCGTCCACATCACACCTCCCCTCAG 59.7 0.23
mm3_exon_Nodal_chr10_61418206_R GTCTCGTGGGCTCGGCCCTTATATCTGAGCCGCCG 60.1 0.23
mm4_intergenic_Gm23325|Pak3_chrX_143462581_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm23325|Pak3_chrX_143462581_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Ccdc116_chr16_17143702_F TCGTCGGCAGCGTCGCTGGGAGACTAAGAAGTGGA 58.8 0.23
mm4_intron_Ccdc116_chr16_17143702_R GTCTCGTGGGCTCGGACAACAGTGTAGGTCTGTGCT 59.2 0.23
mm4_intergenic_Atf3|Gm24748_chr1_191196726_F TCGTCGGCAGCGTCAGCTCCTACAAGGGTGTGGA 60.1 0.23
mm4_intergenic_Atf3|Gm24748_chr1_191196726_R GTCTCGTGGGCTCGGGACCTCAAGTGGCTTTCCCA 59.8 0.23
mm4_intron_Ppp6r3_chr19_3549094_F Primer3: not found at this Tm N.d. 0.23
mm4_intron_Ppp6r3_chr19_3549094_R Primer3: not found at this Tm N.d. 0.23
mm4_intron_Plg_chr17_12380397_F TCGTCGGCAGCGTCTCCACACTCTCTACCCAGCT 59.5 0.22
mm4_intron_Plg_chr17_12380397_R GTCTCGTGGGCTCGGCAGGATCGGAAGCAGGAACA 59.7 0.22
mm4_intergenic_Gm24122|Gm23036_chr1_102204040_F TCGTCGGCAGCGTCAATGGCCAGGGCTTTCTACC 60.0 0.22
mm4_intergenic_Gm24122|Gm23036_chr1_102204040_R GTCTCGTGGGCTCGGTGCTTCTTTTCATGAACCCTTTCC 59.9 0.22
mm4_intron_Nhsl1_chr10_18392275_F TCGTCGGCAGCGTCATGAGAGCCAGCAGAGGAGA 60.0 0.22
mm4_intron_Nhsl1_chr10_18392275_R GTCTCGTGGGCTCGGCCTGCAACAGAAGTGGGAGT 59.8 0.22
mm4_intergenic_Gfpt1|D6Ertd527e_chr6_87099371_F TCGTCGGCAGCGTCGATGCTGATAGGCCAGGCTG 60.6 0.22
mm4_intergenic_Gfpt1|D6Ertd527e_chr6_87099371_R GTCTCGTGGGCTCGGCCACCTGCTCACAAAGGCTA 59.9 0.22
mm4_intergenic_Rgag1|Gm4760_chrX_143181210_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Rgag1|Gm4760_chrX_143181210_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Psme2_chr14_55588389_F TCGTCGGCAGCGTCTTCTTCCTCTGACCTCCGGG 60.6 0.21
mm4_intron_Psme2_chr14_55588389_R GTCTCGTGGGCTCGGTTTGGGGTGGCAATTCAGGT 60.1 0.21
mm4_intergenic_Vegfa|Mrps18a_chr17_46041186_F TCGTCGGCAGCGTCGTGAGCCAGAGACCAAGAGG 59.7 0.21
mm4_intergenic_Vegfa|Mrps18a_chr17_46041186_R GTCTCGTGGGCTCGGGGGAGAGTCAGGCCAGGATA 60.1 0.21
mm2_intron_Col13a1_chr10_61963363_F Primer3: not found at this Tm N.d. 0.21
mm2_intron_Col13a1_chr10_61963363_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Gm3054_chr5_137165149_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Gm3054_chr5_137165149_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm23624|4930593C16Rik_chr9_120796991_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm23624|4930593C16Rik_chr9_120796991_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Gm22797|Robo1_chr16_71834159_F TCGTCGGCAGCGTCGTCTGTAAAGGAGCCTGGGG 59.7 0.20
mm4_intergenic_Gm22797|Robo1_chr16_71834159_R GTCTCGTGGGCTCGGACCAGCTTGCTGTTATCCCC 60.0 0.20
mm4_intergenic_Gm24963|Gm10742_chr3_17458730_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Gm24963|Gm10742_chr3_17458730_R Primer3: not found at this Tm N.d. 0.20
mm4_intron_Lep_chr6_29060387_F TCGTCGGCAGCGTCTGTGGACAGCTTGGTGTTGA 59.7 0.20
mm4_intron_Lep_chr6_29060387_R GTCTCGTGGGCTCGGATGCCTTTCAGCTCCCAACA 59.8 0.20
mm4_intergenic_Six2|Srbd1_chr17_85696622_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Six2|Srbd1_chr17_85696622_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Slc22a27|Gm24951_chr19_7942484_F TCGTCGGCAGCGTCTCTCCACTTGTCTATCCCTGAGA 59.7 0.18
mm4_intergenic_Slc22a27|Gm24951_chr19_7942484_R GTCTCGTGGGCTCGGTCTGATAGTTTGGGGAGGGGA 59.6 0.18
mm4_intron_Abr_chr11_76437296_F TCGTCGGCAGCGTCCCGGACTGTTAGTGGAGCAG 60.1 0.18
mm4_intron_Abr_chr11_76437296_R GTCTCGTGGGCTCGGCTCAGACTCTGCTGCTTCCC 60.1 0.18
mm4_intergenic_Ifit1|Slc16a12_chr19_34655392_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Ifit1|Slc16a12_chr19_34655392_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Nr4a3_chr4_48059927_F TCGTCGGCAGCGTCAGCTACTGCCCTCAGAGTGA 59.9 0.18
mm4_intron_Nr4a3_chr4_48059927_R GTCTCGTGGGCTCGGAGAGTTGGAAGAGACTCCATGTC 59.4 0.18
mm4_intron_Iffo2_chr4_139536364_F TCGTCGGCAGCGTCCTACAGCCATCTGCAGGCAT 60.1 0.18
mm4_intron_Iffo2_chr4_139536364_R GTCTCGTGGGCTCGGTCACATTTCCGAGACTCCCC 59.0 0.18
mm4_intergenic_Gm15208|Bcl11b_chr12_107814195_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm15208|Bcl11b_chr12_107814195_R Primer3: not found at this Tm N.d. 0.18
mm4_intron_Astn2_chr4_65535486_F Primer3: not found at this Tm N.d. 0.17
mm4_intron_Astn2_chr4_65535486_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm13232|Gm13244_chr4_145745016_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm13232|Gm13244_chr4_145745016_R Primer3: not found at this Tm N.d. 0.17
mm4_intron_Zfp423_chr8_87806811_F TCGTCGGCAGCGTCGGGCCATCTCTCCACTCAAC 60.1 0.17
mm4_intron_Zfp423_chr8_87806811_R GTCTCGTGGGCTCGGCCCTGTGCCTTCTTTCTGGT 59.8 0.17
mm4_intergenic_Gm25475|Gm27029/Aarsd1_chr11_101406150_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm25475|Gm27029/Aarsd1_chr11_101406150_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_A830012C17Rik|Gm11780_chr4_4424435_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_A830012C17Rik|Gm11780_chr4_4424435_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Elac1|Me2_chr18_73760797_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Elac1|Me2_chr18_73760797_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm22597|Gm24514_chr18_55410021_F TCGTCGGCAGCGTCTGTGGCAGATGTGAAGGTCG 60.3 0.16
mm4_intergenic_Gm22597|Gm24514_chr18_55410021_R GTCTCGTGGGCTCGGTTTTGCTGTCTCACCGTTGC 59.6 0.16
mm4_intergenic_Gm22618|Gm22976_chr5_50435161_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm22618|Gm22976_chr5_50435161_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm10152|Fgf3_chr7_144794357_F TCGTCGGCAGCGTCCCAGGTTTGTGAGTGTGGGA 59.8 0.16
mm4_intergenic_Gm10152|Fgf3_chr7_144794357_R GTCTCGTGGGCTCGGTGGGAGTAAGGCTCTGCTCT 59.9 0.16
mm4_intron_Rbms3_chr9_117176134_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Rbms3_chr9_117176134_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_4930526D03Rik|Oprl1_chr2_181708877_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_4930526D03Rik|Oprl1_chr2_181708877_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_F730016J06Rik|Olfm1_chr2_28134443_F TCGTCGGCAGCGTCCTGTCCTTCACACTTCTGGCT 59.9 0.15
mm4_intergenic_F730016J06Rik|Olfm1_chr2_28134443_R GTCTCGTGGGCTCGGCGAGGGTTTGGAACAGTTGC 59.6 0.15
mm4_intergenic_Sec16b|Brinp2_chr1_157970251_F TCGTCGGCAGCGTCATTCAGGGACCTGGGAGTCA 59.8 0.15
mm4_intergenic_Sec16b|Brinp2_chr1_157970251_R GTCTCGTGGGCTCGGAGGGGAATTGGAAGAGCAGC 60.0 0.15
mm4_intron_Col1a1_chr11_94936876_F TCGTCGGCAGCGTCAAAGGCTGCGGGATGATTCA 60.0 0.15
mm4_intron_Col1a1_chr11_94936876_R GTCTCGTGGGCTCGGTTTCTCATCCAGCAACCCGC 60.9 0.15
mm4_intergenic_Dgkg|mmu-mir-8095_chr16_22521499_F TCGTCGGCAGCGTCGCCAGTGGAGAAATCCTGCT 60.0 0.15
mm4_intergenic_Dgkg|mmu-mir-8095_chr16_22521499_R GTCTCGTGGGCTCGGTGGTCTCTAGTCATTGCGGC 59.8 0.15
mm4_intron_Aprt_chr8_122575905_F TCGTCGGCAGCGTCCACTTACCAGGGCCACTCTG 60.0 0.15
mm4_intron_Aprt_chr8_122575905_R GTCTCGTGGGCTCGGACAACTGCTGTCTACCTGGC 59.9 0.15
mm3_intergenic_Phf15|Gm26551_chr11_51948423_F Primer3: not found at this Tm N.d. 0.14
mm3_intergenic_Phf15|Gm26551_chr11_51948423_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Elfn2_chr15_78675390_F TCGTCGGCAGCGTCTTGTCTCAGCTTCCATGGGG 59.6 0.14
mm4_intron_Elfn2_chr15_78675390_R GTCTCGTGGGCTCGGTAGTCCCCTACTCTTGCCCC 60.0 0.14
mm4_intergenic_Nfatc1|Atp9b_chr18_80734096_F TCGTCGGCAGCGTCGCCGGTTTCCCTGCATACAT 60.7 0.14
mm4_intergenic_Nfatc1|Atp9b_chr18_80734096_R GTCTCGTGGGCTCGGGGTCCTGGTCTTGGTCTCAC 59.6 0.14
mm4_intergenic_Papolg/Gm12061|Gm12062_chr11_23914120_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Papolg/Gm12061|Gm12062_chr11_23914120_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm6253|Zfp637_chr6_117834313_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm6253|Zfp637_chr6_117834313_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_4930583I09Rik|Gm25344_chr17_64923300_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_4930583I09Rik|Gm25344_chr17_64923300_R Primer3: not found at this Tm N.d. 0.14
mm4_intron_Dohh_chr10_81386573_F TCGTCGGCAGCGTCGGCCACATCTGGTTACTAGGG 59.8 0.14
mm4_intron_Dohh_chr10_81386573_R GTCTCGTGGGCTCGGTCGACAGTGAACAGACAAGGG 59.9 0.14
mm4_exon_Zbtb39/Gpr182_chr10_127742012_F TCGTCGGCAGCGTCCAGCCAGTGTGTCACTCTCA 59.6 0.13
mm4_exon_Zbtb39/Gpr182_chr10_127742012_R GTCTCGTGGGCTCGGTGCATCAGTAGGCAGCACAT 59.7 0.13
mm3_intergenic_Zfp710/AC110903.1|Zfp710_chr7_80072536_F Primer3: not found at this Tm N.d. 0.13
mm3_intergenic_Zfp710/AC110903.1|Zfp710_chr7_80072536_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Slc1a2_chr2_102685513_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Slc1a2_chr2_102685513_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm17384|3110047P20Rik/Gm9954_chr5_63124569_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm17384|3110047P20Rik/Gm9954_chr5_63124569_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Phf15|Gm26551_chr11_51891397_F Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Phf15|Gm26551_chr11_51891397_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Dlg4_chr11_70018692_F Primer3: not found at this Tm N.d. 0.13
mm4_exon_Dlg4_chr11_70018692_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Aldh7a1_chr18_56562279_F Primer3: not found at this Tm N.d. 0.13
mm4_exon_Aldh7a1_chr18_56562279_R Primer3: not found at this Tm N.d. 0.13
mm3_exon_Rapgef4_chr2_72141136_F TCGTCGGCAGCGTCTTGGGTGCTGTCGTATAGGC 59.8 0.13
mm3_exon_Rapgef4_chr2_72141136_R GTCTCGTGGGCTCGGAAGCGAGGCAGAAAGAGTGG 60.3 0.13
mm4_intron_Slco4a1_chr2_180458874_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Slco4a1_chr2_180458874_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Glis1|Dmrtb1_chr4_107650018_F TCGTCGGCAGCGTCACCTGTGAGACAAAGCCTCG 59.9 0.12
mm4_intergenic_Glis1|Dmrtb1_chr4_107650018_R GTCTCGTGGGCTCGGTCCATACTTCCCGTTTCTGTCC 59.7 0.12
mm4_intergenic_Sorcs2|Psapl1_chr5_36190368_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Sorcs2|Psapl1_chr5_36190368_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Igkv9-124|Igkv9-123_chr6_67945198_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Igkv9-124|Igkv9-123_chr6_67945198_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm24911|Igkv9-129_chr6_67837952_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm24911|Igkv9-129_chr6_67837952_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Tmod4|Scnm1_chr3_95129483_F TCGTCGGCAGCGTCGTGCCTAGCCACAGTGTGAT 60.0 0.11
mm4_intergenic_Tmod4|Scnm1_chr3_95129483_R GTCTCGTGGGCTCGGCAGCCCGGTGCCCTATTAAT 59.8 0.11
mm4_intergenic_Fam196a|Dock1_chr7_134989934_F TCGTCGGCAGCGTCTCCACTTCCTTTCTCCCAAGC 59.9 0.11
mm4_intergenic_Fam196a|Dock1_chr7_134989934_R GTCTCGTGGGCTCGGGGCCAATCCCACTGTGAGAT 59.7 0.11
mm4_intergenic_Gm10451|Mir5135_chr12_76496225_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm10451|Mir5135_chr12_76496225_R Primer3: not found at this Tm N.d. 0.11
mm4_exon_Ece1_chr4_137963008_F TCGTCGGCAGCGTCAAAAGGGAAGGAGCTGCAGC 60.8 0.10
mm4_exon_Ece1_chr4_137963008_R GTCTCGTGGGCTCGGGTGGTGAGGCTCCATCCATC 60.1 0.10
mm4_intergenic_4930430O22Rik|Msi1_chr5_115436909_F TCGTCGGCAGCGTCCACTGGTCAGCAGGCCTC 60.0 0.10
mm4_intergenic_4930430O22Rik|Msi1_chr5_115436909_R GTCTCGTGGGCTCGGGGCTTTGGTCTCCATGGGAA 59.9 0.10
mm4_intergenic_Eif3b|Chst12_chr5_140485966_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Eif3b|Chst12_chr5_140485966_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Sst|Rtp2_chr16_23893942_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Sst|Rtp2_chr16_23893942_R Primer3: not found at this Tm N.d. 0.10
mm4_exon_Tmem74b_chr2_151702122_F Primer3: not found at this Tm N.d. 0.10
mm4_exon_Tmem74b_chr2_151702122_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Zfy2|Gm22119_chrY_2424260_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Zfy2|Gm22119_chrY_2424260_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Col23a1_chr11_51459629_F TCGTCGGCAGCGTCGACTGAGGGCTCTTTGGCAT 60.0 0.09
mm4_intron_Col23a1_chr11_51459629_R GTCTCGTGGGCTCGGAGCAACCCTGTCCTTCACTG 59.8 0.09
mm4_intergenic_Olfr519|Nlrp10_chr7_108899323_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Olfr519|Nlrp10_chr7_108899323_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29408113_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Cd274|Pdcd1lg2_chr19_29408113_R Primer3: not found at this Tm N.d. 0.09
mm3_intron_Kank4_chr4_98760500_F TCGTCGGCAGCGTCCTTGCTGCTGGTCACTCTGA 59.9 0.09
mm3_intron_Kank4_chr4_98760500_R GTCTCGTGGGCTCGGTGCTATCAACTGGCATCTCTGT 59.4 0.09
mm4_intergenic_Snd1|Gm3294_chr6_28947410_F TCGTCGGCAGCGTCTGGAGGAGAAAAGGCTTGTCA 59.2 0.09
mm4_intergenic_Snd1|Gm3294_chr6_28947410_R GTCTCGTGGGCTCGGAGCTCTGACGGACCACTTTG 59.9 0.09
mm4_intron_Atp2a3_chr11_72976631_F TCGTCGGCAGCGTCTGGCTTGAGAATGAGCTGCA 59.9 0.09
mm4_intron_Atp2a3_chr11_72976631_R GTCTCGTGGGCTCGGCTCAGAGCCAGTCCCTACCT 60.0 0.09
mm4_intron_Nxph1_chr6_9036940_F TCGTCGGCAGCGTCTGTGCTCACCAGAACTTGTGT 60.0 0.09
mm4_intron_Nxph1_chr6_9036940_R GTCTCGTGGGCTCGGCCCAGTCTCTCCTAACATCATGG 59.9 0.09
mm4_intergenic_Vstm2b|4930433I11Rik_chr7_40945309_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Vstm2b|4930433I11Rik_chr7_40945309_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_MIMT1_1|Mir3099_chr7_6787932_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_MIMT1_1|Mir3099_chr7_6787932_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm5449|Drd1a_chr13_53766261_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm5449|Drd1a_chr13_53766261_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Timm8a1_chrX_134541356_F TCGTCGGCAGCGTCTCGGCTCGGCATAAGGAATC 59.9 0.08
mm4_intron_Timm8a1_chrX_134541356_R GTCTCGTGGGCTCGGCCTACTTTTCCCGTGCCCTT 59.9 0.08
mm4_intergenic_AU018091|Mir290_chr7_3217735_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_AU018091|Mir290_chr7_3217735_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Rap1gap_chr4_137711873_F TCGTCGGCAGCGTCCTCTGGGCCACACTCTCTTC 59.7 0.08
mm4_intron_Rap1gap_chr4_137711873_R GTCTCGTGGGCTCGGCCCAGGGCTATGAGGTCCTA 59.8 0.08
mm4_exon_Slc7a4/P2rx6_chr16_17573746_F Primer3: not found at this Tm N.d. 0.08
mm4_exon_Slc7a4/P2rx6_chr16_17573746_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Wsb2_chr5_117365252_F Primer3: not found at this Tm N.d. 0.08
mm4_exon_Wsb2_chr5_117365252_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Syce1l|Adamts18_chr8_113688101_F TCGTCGGCAGCGTCGATAGACAGGACAGAGCGGC 59.9 0.07
mm4_intergenic_Syce1l|Adamts18_chr8_113688101_R GTCTCGTGGGCTCGGAGAGGGAGATACAGCCTGCA 60.0 0.07
mm4_exon_Zfp280b_chr10_76041779_F TCGTCGGCAGCGTCAGAGGTTATCCCCAGGCCTT 59.9 0.07
mm4_exon_Zfp280b_chr10_76041779_R GTCTCGTGGGCTCGGTCAATGGTCAAGCACGGAGC 60.9 0.07
mm4_intron_Map4k3_chr17_80646218_F TCGTCGGCAGCGTCCACAAGCTGGGACCCCATAC 60.3 0.07
mm4_intron_Map4k3_chr17_80646218_R GTCTCGTGGGCTCGGATAAAACAGACCGCCGCCAT 60.3 0.07
mm4_exon_C230012O17Rik_chr4_24964047_F TCGTCGGCAGCGTCACACATGATGCTTTGTGATCCC 59.2 0.07
mm4_exon_C230012O17Rik_chr4_24964047_R GTCTCGTGGGCTCGGTCAGTTGTGTGCCTGCTTCT 59.8 0.07
mm4_intergenic_Gm4922|Perp_chr10_18837333_F TCGTCGGCAGCGTCAAGCACCCAATCCTCCAGAG 59.3 0.07
mm4_intergenic_Gm4922|Perp_chr10_18837333_R GTCTCGTGGGCTCGGGTTGGTTTCACGCAGGTCAC 59.9 0.07
mm4_intron_Pde4b_chr4_102504118_F TCGTCGGCAGCGTCCAGATCCTTAGGCCCTGCAC 60.1 0.07
mm4_intron_Pde4b_chr4_102504118_R GTCTCGTGGGCTCGGAAGCAAGCATCAGCCTCACT 59.9 0.07
mm4_intergenic_Appl2|1500009L16Rik_chr10_83695392_F TCGTCGGCAGCGTCACAAAACCAAAGGCTCAGAGA 57.7 0.07
mm4_intergenic_Appl2|1500009L16Rik_chr10_83695392_R GTCTCGTGGGCTCGGAGTCAGAACAGTGCAGCAGG 60.2 0.07
mm4_intron_Rgs22_chr15_36138445_F TCGTCGGCAGCGTCGCAGATGTGGGGATGGCATA 59.8 0.07
mm4_intron_Rgs22_chr15_36138445_R GTCTCGTGGGCTCGGCTCCCCACTCCTGTGAGGTA 59.9 0.07
mm4_intron_Dlgap2_chr8_14657981_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Dlgap2_chr8_14657981_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Map3k3_chr11_106123792_F TCGTCGGCAGCGTCAGCTCACACTTTTGCCTCCT 59.5 0.07
mm4_exon_Map3k3_chr11_106123792_R GTCTCGTGGGCTCGGGCAGACTGGGAAGGCTTGAT 60.0 0.07
mm4_intergenic_Cacul1/Gm25238|Nanos1_chr19_60709424_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Cacul1/Gm25238|Nanos1_chr19_60709424_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Igf2bp2_chr16_22162457_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Igf2bp2_chr16_22162457_R Primer3: not found at this Tm N.d. 0.07
mm3_intergenic_Cmtm6|Cmtm7_chr9_114750403_F TCGTCGGCAGCGTCTCGCTAATGCCCACCCATTT 60.0 0.06
mm3_intergenic_Cmtm6|Cmtm7_chr9_114750403_R GTCTCGTGGGCTCGGGCTCATGGGGAACTCTGGAG 59.8 0.06
mm4_intergenic_Slco6c1|D1Ertd622e_chr1_97366528_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Slco6c1|D1Ertd622e_chr1_97366528_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Asah1_chr8_41369325_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Asah1_chr8_41369325_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Map4_chr9_109932036_F TCGTCGGCAGCGTCCACTAACCTCCACGACTCCC 59.4 0.06
mm4_intron_Map4_chr9_109932036_R GTCTCGTGGGCTCGGCCCCTCTCTCAACCACCCC 61.3 0.06
mm4_intergenic_Mir759|Pcdh8_chr14_79757525_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Mir759|Pcdh8_chr14_79757525_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Gm16722_chr3_30038826_F TCGTCGGCAGCGTCTTCACCAGCATTGAAGGACC 58.0 0.06
mm4_intron_Gm16722_chr3_30038826_R GTCTCGTGGGCTCGGTGGCAGTTGGGGTTCTAAAGT 59.5 0.06
mm4_intergenic_Cpvl|Chn2_chr6_54018339_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Cpvl|Chn2_chr6_54018339_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm13061|Zfp683_chr4_134035944_F TCGTCGGCAGCGTCAATTGGACAGTGGTCGCTGG 60.6 0.05
mm4_intergenic_Gm13061|Zfp683_chr4_134035944_R GTCTCGTGGGCTCGGCTCTCCTCCCAACCTCGGTA 60.0 0.05
mm4_intergenic_Gm15219|Cdkn3_chr14_46578479_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm15219|Cdkn3_chr14_46578479_R Primer3: not found at this Tm N.d. 0.05
mm2_intergenic_Fam174a|Gm15427_chr1_95449331_F TCGTCGGCAGCGTCACGTAGGCTAATTAGAAACATGCT 58.3 0.05
mm2_intergenic_Fam174a|Gm15427_chr1_95449331_R GTCTCGTGGGCTCGGTGAGCCATTGCAGCAGAGAA 59.9 0.05
mm4_intergenic_Eftud1|4933406J10Rik_chr7_82725045_F TCGTCGGCAGCGTCACGCTGTTTTAGTATGCTGCC 59.2 0.05
mm4_intergenic_Eftud1|4933406J10Rik_chr7_82725045_R GTCTCGTGGGCTCGGCAGCCAGGTACCTTCTGTGG 60.0 0.05
mm4_intron_Cacna1g_chr11_94439244_F TCGTCGGCAGCGTCACACCCTCCTTACATCCTCCA 59.9 0.04
mm4_intron_Cacna1g_chr11_94439244_R GTCTCGTGGGCTCGGTTTTGCCCTTCGCTCATTGC 60.0 0.04
mm4_intergenic_Gm22038|Rbm46_chr3_82799231_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22038|Rbm46_chr3_82799231_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Kdm3a|Reep1_chr6_71651558_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Kdm3a|Reep1_chr6_71651558_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Als2cl|Gm590_chr9_110901058_F TCGTCGGCAGCGTCTAAAGGGTACGGGCTGTGTG 59.6 0.04
mm4_intergenic_Als2cl|Gm590_chr9_110901058_R GTCTCGTGGGCTCGGGGACACTAGATGCCCAGCTT 59.4 0.04
mm4_intergenic_Gm9025|Gm15322_chr13_111982949_F TCGTCGGCAGCGTCGAGAGAAGACAGCAGCTGGC 60.7 0.04
mm4_intergenic_Gm9025|Gm15322_chr13_111982949_R GTCTCGTGGGCTCGGATGGTAGGTGCTCAGGAACC 59.0 0.04
mm4_intergenic_Mtus2|Gm10167_chr5_148136703_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Mtus2|Gm10167_chr5_148136703_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Trhr2|Gm20681_chr8_122363270_F TCGTCGGCAGCGTCGTCCCAGGGCCAATCTGTAC 60.1 0.03
mm4_intergenic_Trhr2|Gm20681_chr8_122363270_R GTCTCGTGGGCTCGGCAAGCCCAAGAGGACAGTGT 59.8 0.03
mm4_intron_Gm16013_chr11_71435375_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Gm16013_chr11_71435375_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm24120|Nr2f2_chr7_69995549_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm24120|Nr2f2_chr7_69995549_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm6594|Pkdcc_chr17_82946322_F TCGTCGGCAGCGTCGCCTGTTAAAGCACTGTGCC 60.0 0.03
mm4_intergenic_Gm6594|Pkdcc_chr17_82946322_R GTCTCGTGGGCTCGGGTGGGAGAGCCAAGGAGAAG 59.7 0.03
mm4_intron_Spock1_chr13_57543734_F TCGTCGGCAGCGTCACAGACGAGGGAGCCTACTT 59.9 0.03
mm4_intron_Spock1_chr13_57543734_R GTCTCGTGGGCTCGGAATGTGTTCCAGTGGGCAGT 59.8 0.03
mm4_intron_Pitx1_chr13_55833964_F TCGTCGGCAGCGTCGGTTCACTGAAGCAGGTCGA 59.9 0.02
mm4_intron_Pitx1_chr13_55833964_R GTCTCGTGGGCTCGGCCCACAACAATGCAGGCATC 60.1 0.02
mm4_intron_Dcdc5_chr2_106048607_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Dcdc5_chr2_106048607_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Fbxo16|Zfp395_chr14_65345441_F TCGTCGGCAGCGTCACGGACATGAGAAATTTAGAGACT 57.7 0.02
mm4_intergenic_Fbxo16|Zfp395_chr14_65345441_R GTCTCGTGGGCTCGGGCACACTGGTCAAGAGCTCT 59.9 0.02
mm4_intron_Adcy2_chr13_68772837_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Adcy2_chr13_68772837_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4930569F06Rik|Suds3_chr5_116827851_F TCGTCGGCAGCGTCCCAACCAGCACATTTGGCTG 60.3 0.02
mm4_intergenic_4930569F06Rik|Suds3_chr5_116827851_R GTCTCGTGGGCTCGGGCAGAATTGAGGGCAGAAGC 59.5 0.02
mm4_intron_Ifngr2_chr16_91560330_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Ifngr2_chr16_91560330_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm24070|Gm24994_chr12_77906180_F TCGTCGGCAGCGTCGGGAGAGAACAGGAGGCCTA 60.0 0.02
mm4_intergenic_Gm24070|Gm24994_chr12_77906180_R GTCTCGTGGGCTCGGTGATTAGCTAAGAGGGAGCGG 59.0 0.02
mm4_intergenic_1700001J03Rik|Rnf6_chr5_146203060_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_1700001J03Rik|Rnf6_chr5_146203060_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Pax7_chr4_139754747_F TCGTCGGCAGCGTCAATAAGGGGCCCAGCTTCAC 60.0 0.01
mm4_intron_Pax7_chr4_139754747_R GTCTCGTGGGCTCGGCCACAGAGGAGAGGTTGCAG 60.0 0.01
mm4_intergenic_Gm14643|Gm14575_chrX_40584619_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm14643|Gm14575_chrX_40584619_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Ttll6_chr11_96146343_F TCGTCGGCAGCGTCAAGCTTTCCCAGAAGCTGCT 59.8 0.00
mm4_intron_Ttll6_chr11_96146343_R GTCTCGTGGGCTCGGAGAGGAGGCAGCTAGAAGGC 61.0 0.00
mm4_intron_Tmem40_chr6_115742045_F TCGTCGGCAGCGTCTTCCTTCAACAGCAGGCACA 60.1 0.00
mm4_intron_Tmem40_chr6_115742045_R GTCTCGTGGGCTCGGCACACTTCCTAGTCGGGCAG 60.1 0.00
mm4_intron_Tln2_chr9_67502083_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Tln2_chr9_67502083_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3gl3_chr7_82300388_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Sh3gl3_chr7_82300388_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Parva_chr7_112557587_F TCGTCGGCAGCGTCCCTAGCTCTCACACCCTCCT 60.0 0.00
mm4_intron_Parva_chr7_112557587_R GTCTCGTGGGCTCGGCTAGACTGCTGGTGACCGAC 59.8 0.00
mm4_intron_Otog_chr7_46304313_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Otog_chr7_46304313_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Micalcl_chr7_112373753_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Micalcl_chr7_112373753_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Lrfn2_chr17_48948457_F TCGTCGGCAGCGTCGGCAGTACAAAGTGGAGGCT 59.9 0.00
mm4_intron_Lrfn2_chr17_48948457_R GTCTCGTGGGCTCGGGATGGAGGGATGCTGAGCTC 59.9 0.00
mm4_intron_Lpin1_chr12_16543399_F TCGTCGGCAGCGTCTGAGCAGTGGGGTTTGACAG 60.1 0.00
mm4_intron_Lpin1_chr12_16543399_R GTCTCGTGGGCTCGGGCACGCATACACACTTGCTC 60.1 0.00
mm4_intron_Ifltd1_chr6_145550135_F TCGTCGGCAGCGTCTGTCTTTTAATCTCCCAGTGTCT 57.1 0.00
mm4_intron_Ifltd1_chr6_145550135_R GTCTCGTGGGCTCGGCCATGGCTCTTTGGGACTCA 59.6 0.00
mm4_intron_Fars2_chr13_36489866_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fars2_chr13_36489866_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Aff1_chr5_103749978_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Aff1_chr5_103749978_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_A230108P19Rik_chr2_6300007_F TCGTCGGCAGCGTCCTTCCTCCCCTCCCAGGTAG 60.3 0.00
mm4_intron_A230108P19Rik_chr2_6300007_R GTCTCGTGGGCTCGGATACTTTGGGATGTGCCGCA 60.0 0.00
mm4_intergenic_Zfp957|Rgcc_chr14_79281620_F TCGTCGGCAGCGTCGAGTCCCTCGGTCATGGTTC 59.8 0.00
mm4_intergenic_Zfp957|Rgcc_chr14_79281620_R GTCTCGTGGGCTCGGAGACGATTTAGTGCCAGCTCA 59.4 0.00
mm4_intergenic_Scgb1b27|Gm12760_chr7_34023936_F TCGTCGGCAGCGTCGCCAAGTCAGTTTGTGGCTG 59.9 0.00
mm4_intergenic_Scgb1b27|Gm12760_chr7_34023936_R GTCTCGTGGGCTCGGCCCGTCATAGGGTCTCAAGA 58.2 0.00
mm4_intergenic_Napa|Kptn_chr7_16118779_F TCGTCGGCAGCGTCACCTCAGTCCAGATCCTGCT 59.9 0.00
mm4_intergenic_Napa|Kptn_chr7_16118779_R GTCTCGTGGGCTCGGTTGTCCACTCACACTGCTCC 59.8 0.00
mm4_intergenic_Kif2b|Gm11498_chr11_91632236_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Kif2b|Gm11498_chr11_91632236_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Igfbp5|Tnp1_chr1_72877286_F TCGTCGGCAGCGTCAGTCCATTCTCTCTGCCCTCT 59.9 0.00
mm4_intergenic_Igfbp5|Tnp1_chr1_72877286_R GTCTCGTGGGCTCGGTCTCTGAGAGTGCGACCAGA 59.9 0.00
mm4_intergenic_Gm8682|Map3k15_chrX_160036962_F TCGTCGGCAGCGTCCTCCTTTCCTGTCAGGCTGG 60.0 0.00
mm4_intergenic_Gm8682|Map3k15_chrX_160036962_R GTCTCGTGGGCTCGGGTTTGGGAAGGCCTGAACCT 60.1 0.00
mm4_intergenic_Gm6180|Gm23531_chr8_42247557_F TCGTCGGCAGCGTCCTTTCCTGCCAGACCTGAGG 60.0 0.00
mm4_intergenic_Gm6180|Gm23531_chr8_42247557_R GTCTCGTGGGCTCGGTTGGGAAGGCCAGAACAGTC 59.8 0.00
mm4_intergenic_Gm26338|Tspan9_chr6_128122571_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26338|Tspan9_chr6_128122571_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23829|Gm5974_chr1_48166822_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23829|Gm5974_chr1_48166822_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23293|Gm11202_chr11_79600556_F TCGTCGGCAGCGTCTCCCCTCTCACAGTGATCCC 60.3 0.00
mm4_intergenic_Gm23293|Gm11202_chr11_79600556_R GTCTCGTGGGCTCGGCCTTAGGGCTTCGGTTCCAG 60.1 0.00
mm4_intergenic_Glb1|Ccr4_chr9_114476589_F TCGTCGGCAGCGTCTGTGAGGGATCTAAGCTGTGG 59.1 0.00
mm4_intergenic_Glb1|Ccr4_chr9_114476589_R GTCTCGTGGGCTCGGACACAGTTGCCTAGAGCCAG 59.6 0.00
mm4_intergenic_Csmd1|Gm25665_chr8_18051947_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Csmd1|Gm25665_chr8_18051947_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ajap1|Gm25880_chr4_153694744_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ajap1|Gm25880_chr4_153694744_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Adra1b|Gm12153_chr11_43915272_F TCGTCGGCAGCGTCAGCACAGGGTTCCTTGGAAG 59.8 0.00
mm4_intergenic_Adra1b|Gm12153_chr11_43915272_R GTCTCGTGGGCTCGGAGTCTCTGCAGCCTGTAGGA 59.9 0.00
mm4_intergenic_Adam34|Gm24591_chr8_43817293_F TCGTCGGCAGCGTCGTGTGCACTGTCCCTACTGG 60.3 0.00
mm4_intergenic_Adam34|Gm24591_chr8_43817293_R GTCTCGTGGGCTCGGGGTTTGGGGACTACCACCAT 59.2 0.00
mm4_intergenic_Ackr4/Acad11|Dnajc13_chr9_104146865_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Ackr4/Acad11|Dnajc13_chr9_104146865_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Prdm14_chr1_13125808_F TCGTCGGCAGCGTCGCATGCGCGTAGTAGGTAGT 59.9 0.00
mm4_exon_Prdm14_chr1_13125808_R GTCTCGTGGGCTCGGCAGCTCGGTTTCCCACAGAT 60.0 0.00
mm4_exon_Cfl1_chr19_5493639_F TCGTCGGCAGCGTCTCACCCCAGTTGCCAAACAT 60.1 0.00
mm4_exon_Cfl1_chr19_5493639_R GTCTCGTGGGCTCGGCAGAACCGTCAGGGATGGAG 59.8 0.00
mm3_intron_Gab3_chrX_74970891_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Gab3_chrX_74970891_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_RP24-234J3.4/Gm21971|Gm21971_chr12_102726024_F TCGTCGGCAGCGTCCGGCAGCCAGTTCACTTAGA 60.0 0.00
mm3_intergenic_RP24-234J3.4/Gm21971|Gm21971_chr12_102726024_R GTCTCGTGGGCTCGGCTACCAAGCAGGAACCCCTC 59.7 0.00
mm3_intergenic_Onecut1/Gm20649|Fam214a_chr9_74907024_F TCGTCGGCAGCGTCCTCTGAGGTCTGTGTGGCTG 60.0 0.00
mm3_intergenic_Onecut1/Gm20649|Fam214a_chr9_74907024_R GTCTCGTGGGCTCGGCCAAGTTCAGGGACCCACTT 59.5 0.00
mm3_intergenic_Gm23558|Galc_chr12_97676888_F TCGTCGGCAGCGTCAGCAGTTTGGGGAAGCTAGA 58.6 0.00
mm3_intergenic_Gm23558|Galc_chr12_97676888_R GTCTCGTGGGCTCGGGAGAGGCTAGAGGGATCCCA 59.5 0.00
mm3_intergenic_Gm14758|Gm14695_chrX_74778199_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Gm14758|Gm14695_chrX_74778199_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Gm14694|Gm6897_chrX_74854864_F TCGTCGGCAGCGTCTTGCTGTGACCCTGCAGAAT 59.8 0.00
mm3_intergenic_Gm14694|Gm6897_chrX_74854864_R GTCTCGTGGGCTCGGGTGTCTGCCTGACTCTGGAC 60.0 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313817 AGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCCCTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAG
GAC
mm4_intron_Txlna_chr4_129637043 ATGTGTTCCTGCACCCTAGCTCCTACCCCAGGTAGCCTGATGACTTTCCAAAGGGAGGGAGGGTATGAAAGGAGGAAAGG
GCATGGACTGACCA
mm3_intergenic_Gm13418|Gm13417_chr2_24513082 ATGCTGGCAGGAAAGAACCAGTAAGGATAGGACTCTCCAGAAAGGAGGAAGGGTGTGAGCTGTTGAATTTTGGGAAATGA
GCCCATGCAGA
mm4_intergenic_Tmem117|Nell2_chr15_95209423 GGCCCAGAGGATAGCAAAGGAATGTCAGCCGGCCGCTGTCCCTCCCTCCCTCTGAAGGGTACCGGAACTTAGGGGGAGCT
CCTGTTTTGACA
mm4_intergenic_Rras2|Copb1_chr7_114169156 GGGGCAGATTTCACCCTCTCTCCTGTTTTGTATCTGGCTCATGCCTATATAGTTCCTGTCACATCTTTACCTTCCTTCCC
TCTGTAGAGTCTGAGCTCTGTGAGAGCAGAGCCCTGGCCTACTGTTCCTCAC
mm4_intron_Adamtsl1_chr4_86388980 ATAGTGCTTGCAGGCCAGAGGCCGGAAACTCTGTCTAGGCCATCTCTCCCTCTAGAGAGTCTATAGCCAACCCAGATAGA
GTTCCCATGGCAAAACCTCCCTGCCATCGAACACTGAGGACCAAGC
mm4_intergenic_Gm24291|Mon1b_chr8_113551663 GCGTTGTTCTGAACCGGAAGAAGTGAAGACAGCTTCCTCCTACCCCCATCTCAGAGCAGAGCAACTTCCTCTTGACTTTC
CCTCCCCTCCCCTGGAGAATCAGGGAGTAGCAGGTAACACTCCAAGGGAGGAAGGAGAGG
mm4_intron_Eif3m_chr2_105015043 ATCTGCCCTGCTTCCAAAGTAACATGCAAGTGCTAACTAACTACCCTCCAATTGTGTTGATTCCCTCCCTCCCTCTACAA
AGTCCACGCAGACACATAGTTCACAAAGATCATATTCAGTTTCCTGCCACCTGTATCCA
mm4_intron_Fam129c_chr8_71602993 ACAGGTGAGAGGTGGAGCTAGGAATCCCCTGAGGGAGGGAGGGAAGGCTGCCTGGTGAGCAGGGTGAGGTCACGGAGCGA
GGGGAGTGGCTTGGGGGTGTTGGCTCAGGGGGCCGAGGGACATGTTTAT
mm4_intron_Myo18b_chr5_112787221 CACGGGTCTGTGGTCTTTGAGCCATTGTAGAAGCAGTCTAGCTCACTTTGAACACTCAATGTTCAGCCAATGCTGACTGA
GCCTTCACTCCCTCTAGTGAGTCCTTCCCGACCTCCCTTAGTCC
mm4_intron_Sntb1_chr15_55785609 AGCCTCAGAGAACCAGGACTAGAAAGTGATGAAATTTGGTAAATGAATTCTTATTCTGCTCTGTTCTGACTCTCCTGGGG
GGGGGGAGGGGAACCATGTGAAATAAAGAAGTTTAATTAGTCATGAAAGGACAGTGAACT
mm2_intron_Ccdc30_chr4_119385354 TGCAAATCGCCAATAGTCGCTACAACATTGCAAATTCTCAAATATCAGCTTCCACCTGTATTAGACAGGGCTCTCCAGAG
AGGGGGAGGGCGGTGAGGGAGGGAAAGACT
mm4_intron_Epb4.1l1_chr2_156509372 GATGGAGGTATGGCTCAGGCTACAGCTGGGGAGAGAGTGGTCCTCAGAGGAGAGGAGGGGCGTGGACAGGGCGGGTTCTC
CAGAGGAGAGGAGGGGCGTGGACAGGGCGGGTTCTCCAGAAGAGACAGCCGGTA
mm4_intron_Tpd52_chr3_8949197 TGCTGTTTAACAATGGAGAGAAGAGTAAATAGAATCTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNAATAAATATGACA
AGTATGTCAGCACAGCACTGAGACTG
mm4_intergenic_Tas1r2|Pax7_chr4_139710612 AAGGCAACAAAGGACCCCAACTGTCCCTCAGGAGGGAGGACTCTCCTGGAGGGGGAAGGGGTGACTTGGTGAGGCAGGCG
ACCTAACTAGGGGTGGGTAATGGGTGACAG
mm4_intron_Slc22a5_chr11_53887776 TGCCATGAAACTGCAAAGGCAAAAAATGAGGGTCTGTGATTCTCCAGAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGCTAGCACAGGCAGG
mm4_intergenic_Dysf|Cyp26b1_chr6_84360426 CCAACACAGGGGTCTTCAGGACACAGGAGTGGTGAGGCAGAGGCCCTCTCCACTTACCCTTCATTCCCTCTGCAGAGTCC
TGAGGAGTGTGTGTGAGTTGTGCTTGCTCTCATATTCCTGGGATGGGAGGAAAAGCA
mm4_exon_Iqgap3_chr3_88107521 TTGCCTGACCAATGTCTCCCCTCCTGCCAGTGCACGCACGCCATCCTCCCCTCTGTGTAGTCCGAAAATTCGCTCACCTC
TTGAACCAG
mm4_intron_Snd1_chr6_28900999 AAAGGCCAAGGAGATGAGGCTGTAATGCACCCTCCAGGGGGGGGGGGGGAGAAACAGAGACCCTTCCCATTCCTATCACA
CACACACCAGGACTT
mm4_intergenic_Rps16|Plekhg2_chr7_28357189 CCTCCTTTGAATGCCGGGAATTCCGGCGCCGCTGGGAGCCCAGGCCAGGCTGGGAACTTTCCTACAGCTGGGGGTGGCTG
GGAATGTGCAACTCTGCAGAGGAGGGGAGGGCCAGCCTCAGTTCTGACCTTGGG
mm4_exon_Pnkd_chr1_74286137 TCTGAAATGCGGGTGGAGAGCTGAATCCAAGGGAAGGAAATTGCTGGAGTCAAGGGATGGACTCTCAGAAGGGGGGGGGG
GGCTAGGGTGGAAAAATATGTAAAATTGGGGGATAGTGGCTTTTCTGTGGAGCA
mm4_intron_Sipa1l3_chr7_29444663 TGGGTGACTCAGAGGAACCTAGAGGCCTGAGGCTCTCCTGAGAGGGGGAGGGAGACCACCAGCAGCAATCACCCATGGAA
mm4_intron_Ap3b1_chr13_94372442 AGAGGAGGGCAGGATGGATTTGGAGTCATCAGAGGAGGGGAGGGGACAAGGTAGGAGGCTGTGACAGAGCTAAGGGGTCC
TGTGGTGACTGAGGGCTTGTCTGACTGCAGCTA
mm4_intergenic_Anapc15-ps|4732465J04Rik_chr10_95763497 TGTTTGTGTACACCCGGAGCGGCCCTCAGAGGAGGTTCTTGAAAGCCCTAAGGGTTAGGGTCACTTGTGAGTTTAAGATT
CTTGGTGTCTGTGATGGGACACTCTTGAGGGGAGGAAGGAAAGCCGGATACAGCCTGAC
mm4_intergenic_Ppt1/Cap1|Cap1_chr4_122861471 GACAACAGCCTGATTTGGGGGGTTAGTCTTATTCTTACTGAACTATGAAAGGCAGGAATGANNNNNNNNNNNNNNNNNNN
NNNNNNTGGAGCTCCCTTCATGGACTACCTGGCTTCT
mm4_intergenic_Atp1a3|Grik5_chr7_25008252 GTGCATAACGCTCCCAATGCGAGACTCTGCAGAAGGGGGGTGGGGAGACTTTGGAAGGTCACAGGGATGGAGGA
mm4_intergenic_Thumpd2|Slc8a1_chr17_81178733 GGAGGGCCCAAATGCTTAGTTGGAGAGGGGATTGGAAACAGTGTTGAAAGCATTCAAAACTTTGGCCTATGCTTCCTTGG
AAGACCCCCCCCCCCTCTGAAATGTCCCATCACCAGTAGTGTTGGGCATGTGTGGG
mm4_intergenic_Wnt11/Gm8398|Uvrag_chr7_98867133 ACAGTGGCTGGAAGGATTGGGTCCAGAATGCTGCCTATTGTCACTCACCCCAGCCCTTCCCCTCCCCTGGAGAGTAGTAG
GACTATGGGCAGTTCACAGAACACCAGAGCCTACCAGG
mm3_intron_Shfm1_chr6_6572277 TGTAGCTATACATTTCCTGTGTCTTTTAATTTTATATGAATAGCACTCTACAGAGGGGGTGAGGGATAATTCAGACTGAA
TGAGGTCATGAAGGTGCGG
mm4_intergenic_Vegfa|Mrps18a_chr17_46110181 AGAGGTGCCAATGTGGGAAACGCTGGCAGTGACTCTTTATAGGGGGGGAGGGGGGAGCGGTGTTGTTTCCATTCATAAAG
AACTAGGGATGCCCTGGGCTTCCCAGGATGCAGCCACAAGGGTGACTAGGGTG
mm4_intergenic_Clec16a|Gm15558_chr16_10655586 CTGCAGTAGTCCCTGTCCCTAAGTACTCTGCAGAGGAGGGGGCGGGCTTTCGTGGTAGCTGCAGCATGGCTTTCT
mm3_intergenic_Gm22251|Zfp521_chr18_13461464 TGTCTCACCCTTCACTCTTCTTCTTTTACTACCCCTACCCCCTCTCTGTAGAGTCCAGAATTCAAAATGGTCTCAGCTCA
TGGCTGTTTAGATTGTTTGTGCCTCTGCGTGATC
mm3_intron_Llgl2_chr11_115855263 GGCTCAGCAATGGAGAAGGTAGGGCTGTGGGCACTCCAGAGGAGGGGAGGGCCTGCTGCCAGGGAGGGATGGGAAGGCGG
TCCTTAAAAGAACTGCCACCCCTCC
mm4_intergenic_Gm5909|Gypa_chr8_80149961 CCCCAGGGCCATCTTTCTTTAATCATTCACTAATGTTTTCCTGGTGAGGTCATTGATTAATGGATGACGTAAATCTCCCA
GCCCCCTCCCCCCCTTAGGACAGTACATAATGTTTTATTATTTTGTCCTTTTGGCTGCTT
mm4_intron_Capn13_chr17_73356143 TGTGAGTTGCCTGGTGTCTTTCACCTGTGAGCTATGACTCCATGGACCCTTCCCCCTCTCTGAAGTGTTCTTCTCTATGT
GTCTGCTGATGTACACCCCTCTGTCCCTCTG
mm4_intron_B230323A14Rik_chr9_69767712 CTTGGCACAGAAGCTGCAAGTCCGGGCACGGCACCCTCTGGGATCTCACTACCCTCACCTCCTCTGGACATTCCAGACGC
TCAGCGGTGGGTCACAGTTGGGATC
mm4_intergenic_Aqp6|Racgap1_chr15_99607471 GTGCTTTGAGGGACGTCTGGGGGCCATTTTTCACTGATAGTCCTTTAGCACCCTCTGTAGGGACGGACCAGAGGAGGGGA
AGGAGAAATGAGGAACTGGGAATCAGGTTAAAGCCGGAGAGC
mm4_intergenic_Gm25130|Gm22970_chr14_99492800 TCCAGAGATTCATGTAGCAGCAATTGTTAAGAAGACTCTCAAGTGGGAGGGAAGGGCAAATGAATCCATCAGTCTGTCTC
TCTTTTCTACTGAAAGAATTAGTGAACTCTTCCCTTGGGG
mm4_intron_Sptlc1_chr13_53344662 ACACAAACCTAGCTGCACCTAGAAGAGACATGAAAAGCCAAGTTATACATACTACCCCCACCCCTGAGAAACAGAACACT
CCAGAGGGAGGGTAGGGTCCTGACTGGGCTGACCAGATCCACCCAGACA
mm4_intron_Apol7d_chr1_71655060 AGGGCTCCTTGTAAAACAGTGAACTTCTGGGAAGTGCTCTCTCCAGGGGGGGAGAAGGATACCTGGAAATCAGGATGCTT
GCTAGATAAACCCGAAGGGAAGGCTTT
mm4_intergenic_Gata3|Gm13262_chr2_9896304 CGGGGCTGGAAATTTGTGTGGGACTCTGCGGGGGAGGGGAGGGCTCAGGGATGGATGGGTGGAT
mm3_exon_Nodal_chr10_61418206 CACATCACACCTCCCCTCAGCAGGGGCTCCCCTGCCCTGCCCCTCCAGGGTGGTTATAAGTTCTTAACCTATAGGTTATA
GGCCTCTCCGGAGGGAGGGAGGGAAAGGGGCGGGGCGGCGGCGGCTCAGATATAAGGG
mm4_intron_Ccdc116_chr16_17143702 GCTGGGAGACTAAGAAGTGGAATTCTGTGTTCATGGTGTGAAAGTGCAATTATGAGAGCCCTCCACCCTCTCTGGAGAGC
CCTTCTTCTCTGTCTCTAGCACAGACCTACACTGTTGT
mm4_intergenic_Atf3|Gm24748_chr1_191196726 AGCTCCTACAAGGGTGTGGAACATGATAGGAAAGGGGCTCTCCTCAGGGAGGGAGGGGAGACCTGTGTAAGAATGCAGAC
ACAAGATGGATGAGTCTGGGAAAGCCACTTGAGGTC
mm4_intron_Plg_chr17_12380397 TCCACACTCTCTACCCAGCTTTCCAGTTTGGAGCATTGAGGAGGGATGGAGTCACCAGAGAGGGAGAGGGTAGGCTTTCA
AAGATGAGTCTGTGTTTACCTTCTCAAGTGTTCCTGCTTCCGATCCTG
mm4_intergenic_Gm24122|Gm23036_chr1_102204040 AATGGCCAGGGCTTTCTACCGCACCTCCCTCCTCCCCTCTACAGAGGCCATGAGCTTAAAGATGAAAAGGAAAGGGTTCA
TGAAAAGAAGCA
mm4_intron_Nhsl1_chr10_18392275 ATGAGAGCCAGCAGAGGAGAGCAAAAGCGGAGAGGGTAGAGTGGGCCCAGTACTCTCCTCAGGGGAGGAGGGAGAATCAA
GTCAAGACTCCCACTTCTGTTGCAGG
mm4_intergenic_Gfpt1|D6Ertd527e_chr6_87099371 GATGCTGATAGGCCAGGCTGCCTGGGAAGTGGATTCTTGTCAGTACCACATCTANNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNCAGAGTCCTCAGGATAATGTGTAGGAGGCTCATAGCCTTTGTGAGCAGGTGG
mm4_intron_Psme2_chr14_55588389 TTCTTCCTCTGACCTCCGGGTGATGGCCTTGTTTCCATCCCTCCCTCTTGAGTCTCCTCCCACTCTGTCTTCAGTCACCA
ATTCTTACCTGAATTGCCACCCCAAA
mm4_intergenic_Vegfa|Mrps18a_chr17_46041186 GTGAGCCAGAGACCAAGAGGCTGGGGAGGCTGAGTGGGGTGTCTACAGAGGGGAGGAAGGAACAGAAAGGCTGTGTGATA
CAAGGCTATCCTGGCCTGACTCTCCC
mm4_intergenic_Gm22797|Robo1_chr16_71834159 GTCTGTAAAGGAGCCTGGGGGAACTCTCCAGGGAGGGGCAGGGAAGAACACTGGGAATCACAGCCCAGCTGGAAGATCTC
ATCTCTATTATTGAAGAATGGAGCTGTCCAGGGGATAACAGCAAGCTGGT
mm4_intron_Lep_chr6_29060387 TGTGGACAGCTTGGTGTTGAATTCAGTAGTTTTGCAGCGAGGGACTCTGCAGACAGAGGGAAGGCTGTTGGGAGCTGAAA
GGCAT
mm4_intergenic_Slc22a27|Gm24951_chr19_7942484 TCTCCACTTGTCTATCCCTGAGAATTTGAATCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNCTCCCATCCCCCCCTCTTGGGCCTCCTCCCCTCCCCAAACTATCAGA
mm4_intron_Abr_chr11_76437296 CCGGACTGTTAGTGGAGCAGTCCCAAAGCAGCGGGTGTGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNCTGGAGACTGCAGCCGGGAAGCAGCAGAGTCTGAG
mm4_intron_Nr4a3_chr4_48059927 AGCTACTGCCCTCAGAGTGAGGTCAACAAAGCAAGGATTAGTCAAGGACAGCCCACGACAGGATTCTCCACAGAGGGGAA
AGGTTGTCTGCAAGACATGGAGTCTCTTCCAACTCT
mm4_intron_Iffo2_chr4_139536364 CTACAGCCATCTGCAGGCATGCAGGGTTCACTGTGGCCCCAGCCTGCCAGAGACTCTCCTGTGGGGGAGAAGGTCTATAA
GGGGGAGTCTCGGAAATGTGA
mm4_intron_Zfp423_chr8_87806811 GGGCCATCTCTCCACTCAACTTGACACCTGGGCTAAATCATTAGGGCAACTGGGGGGATGGGGGGTGGGGAGTGGGGGGA
GTGTCCAGGGGAGGGGATGGAAGCCACCAGAAAGAAGGCACAGGG
mm4_intergenic_Gm22597|Gm24514_chr18_55410021 TGTGGCAGATGTGAAGGTCGCCCTTGTAATGGAATAAGTGAGATGGAGAGTGACCCCCCCCCCCCCCCGGAAGAGTCCTG
ATACAACGGCGTTAGATCTAAGATGGCTCCCAAGCAACGGTGAGACAGCAAAA
mm4_intergenic_Gm10152|Fgf3_chr7_144794357 CCAGGTTTGTGAGTGTGGGAAAGAGAGCATTCTGCAGAGGGGGGCAGGGAGAGGCTGGGAGACACCTGCAAAGCCAGGAG
AGCAGAGCCTTACTCCCA
mm4_intergenic_F730016J06Rik|Olfm1_chr2_28134443 CTGTCCTTCACACTTCTGGCTTCTAAAACTGGCAAAGAACAAATCATCCTTGCAGGAGGGTGGGGAAGAGGGGTCACCCA
GTGGGTTCAAGGGTTCTCCAGTGGAGGGGAAGGGTGTACAGCAACTGTTCCAAACCCTCG
mm4_intergenic_Sec16b|Brinp2_chr1_157970251 ATTCAGGGACCTGGGAGTCAGGCCAGTAGGTGTAATGATGTCTGCAAAGTCCCCGTCCCCCCCTCCCCAGAGTGCCCTGG
AAGAAAATTCCAGGTATTTCTCTTATTGCTGCTGCTCTTCCAATTCCCCT
mm4_intron_Col1a1_chr11_94936876 AAAGGCTGCGGGATGATTCATAAGGAAAGATTGTTTGCCCTCTCTGCAGGCTAGACAGTGTTGCTGGGGCCTCCGGGGTG
CTGGGCTCTCGGGGGGGGGGGAGGGGACGCACGGAGAGGGCGGGTTGCTGGATGAGAAA
mm4_intergenic_Dgkg|mmu-mir-8095_chr16_22521499 GCCAGTGGAGAAATCCTGCTCCAGATGCTACGAAAACTGTCGACAACTCTGGAACTACCAGAACCTGAGGGAGAGGACTC
CTCAGAGGGATGGAAGGCCCGAAGCAGGCCGCAATGACTAGAGACCA
mm4_intron_Aprt_chr8_122575905 CACTTACCAGGGCCACTCTGTTCTCTGGGGTGCCCACCCTCCCTCTGGACACTCCTGGTGGACATTCAGCTGTCCTAGGG
TGTTTCTGAAGCTCAGGCATGGAGCCAGGTAGACAGCAGTTGT
mm4_intron_Elfn2_chr15_78675390 TTGTCTCAGCTTCCATGGGGGAGGGGAGTAGAACGGGCTGGCAGTGGGGGGTGGGAGGGTCTGGGTCTCTCCAGAAGGAG
GGCTGGTTCTGTGGGCAGAACTAAGCCAAAGGAGGAGGGGCAAGAGTAGGGGACTA
mm4_intergenic_Nfatc1|Atp9b_chr18_80734096 GCCGGTTTCCCTGCATACATGTTTTTATGTATAATGAGCAAGGCATATCTACCATGCCGTATGTGTACTGGAAGAACCTG
GGAAGGTTTCCTGCTTTGGAGTTTCCAGTGGGGAGGAAGGGTGAGACCAAGACCAGGACC
mm4_intron_Dohh_chr10_81386573 GGCCACATCTGGTTACTAGGGCAACCTCCTGGAAAAGGAAACTCTCGAGATGGGGGGAGGGGCACCTGTAGGGTCTCCCA
CAGCTCTGGGTCCCCAGCTTTGAGAATTCTACCCTTGTCTGTTCACTGTCGA
mm4_exon_Zbtb39/Gpr182_chr10_127742012 CAGCCAGTGTGTCACTCTCAGTTGTCCCTCAGTAGATCCCACCCACCCCCTTGGAGAGTCCCGGGGGAGTGGGGAACACT
TCGGTCCTGATAGAAACTATGTGCTGCCTACTGATGCA
mm3_exon_Rapgef4_chr2_72141136 TTGGGTGCTGTCGTATAGGCTGATCGTACAATTTATGGCCTCTCTTGTCTCTCTTTCCCCCCCTCTAGGGATTCCTGACA
AGGAGAATGTGAGTAGCCACTCTTTCTGCCTCGCTT
mm4_intergenic_Glis1|Dmrtb1_chr4_107650018 ACCTGTGAGACAAAGCCTCGAGCGTAGCTATGACAGCAGGCCTCTTCAGAGGGGGCAAAGGGGACAGAAACGGGAAGTAT
GGA
mm4_intergenic_Tmod4|Scnm1_chr3_95129483 GTGCCTAGCCACAGTGTGATCTGTGAGAAAAGGACTCACTGGAGGCGGGGAAGGAGTCAGACTCCATGAAGGGCAGCGAC
CTCTTTCTGACAGACTTATTAATAGGGCACCGGGCTG
mm4_intergenic_Fam196a|Dock1_chr7_134989934 TCCACTTCCTTTCTCCCAAGCTCCATATGGTATGGAGAATTCCCATCCCTCCCACTGGACAGTGCACCGAGACTGCCTTT
CTCCCCCCCAGCTCTTCCCGAGAAGCAGATCTCACAGTGGGATTGGCC
mm4_exon_Ece1_chr4_137963008 AAAAGGGAAGGAGCTGCAGCTAGCTCCCAGCGGAGCCATGTTGCCACCCTCCTCCCAGCCCCTCGGCCTAGGGCCCCTTC
CCCACTCTGGAGAGTATGCAGCCATCCTAAGCCTAAGATGGATGGAGCCTCACCAC
mm4_intergenic_4930430O22Rik|Msi1_chr5_115436909 CACTGGTCAGCAGGCCTCGGTCGCCTCCCCTCCCCCCCTCCGGAGCCCCCCTGCCCCCAGGTTCCCATGGAGACCAAAGC
C
mm4_intron_Col23a1_chr11_51459629 GACTGAGGGCTCTTTGGCATCATCTACAAAGGCATCCAAGCTGGGAACAGCTGAGTCTGAGGCAGGCTGCTCTGCACAGC
AGTGACAGGAGGCTCCAGAGGGGGACAGGGGGACAGTGAAGGACAGGGTTGCT
mm3_intron_Kank4_chr4_98760500 CTTGCTGCTGGTCACTCTGATGGCTCCAACAGCATCCAGGAAGAGGTACTGTCCTTGCATAGCAATGGCCTCTCTTTCCC
CCCCTCTAGAGTTTCCGATGCCTTTGATGTTAATTACAGAGATGCCAGTTGATAGCA
mm4_intergenic_Snd1|Gm3294_chr6_28947410 TGGAGGAGAAAAGGCTTGTCAGCCATTAGAGTTTAATGTGAAAGTGTTAACGCGAGACTTTCGCACTCTGCAGAGGAGGG
GCTGGGCTGAGGGGATGGAAGAAGAACTCCGCAAAGTGGTCCGTCAGAGCT
mm4_intron_Atp2a3_chr11_72976631 TGGCTTGAGAATGAGCTGCAATAAATCGGTCTCAGCTGGCTGATGGACCAGGAAATGGACTGTCCTGATGGGGGAAAGGG
CAGGTAGGGACTGGCTCTGAG
mm4_intron_Nxph1_chr6_9036940 TGTGCTCACCAGAACTTGTGTGAGATGATATGCAACAAAGATATTCCTCATTGGTCAGAGATAATTTCTCTAAAAAATAC
TGAGGAATCTCCACTGGGGGGGGGGGACCCTTAACCATGATGTTAGGAGAGACTGGG
mm4_intron_Timm8a1_chrX_134541356 TCGGCTCGGCATAAGGAATCTAAGAACCCGGGGTACAATCAAGACCTCCTCCCACTCTGGAGAGCCCCACTTCCTAGCAA
GTCCTCTGGGTGCAAGGGCACGGGAAAAGTAGG
mm4_intron_Rap1gap_chr4_137711873 CTCTGGGCCACACTCTCTTCCACCCCTCCCCCATCCTTTGCATCCCCCTGAGAGGTGCGGAGCCAAGTGGGTTCCCCAGA
GGGGTGGATGGGAGTCTAGGACCTCATAGCCCTGGG
mm4_intergenic_Syce1l|Adamts18_chr8_113688101 GATAGACAGGACAGAGCGGCAGCACATTCATCTTGCCTCAATCCTATTTCATGTCTTCCGGACTCTGTAGAGGGGCGGTA
GGTGGGAACAATTGATCTATTGAGAATTGCAGGCTGTATCTCCCTCT
mm4_exon_Zfp280b_chr10_76041779 AGAGGTTATCCCCAGGCCTTGGTCTCTCCAGACTGAGGGAGGGACAGAGGTTGTGAGTATCTTTTGTTTGCTCCGTGCTT
GACCATTGA
mm4_intron_Map4k3_chr17_80646218 CACAAGCTGGGACCCCATACGCAGGACCAGGACGTCTGGGGAAGGGGCAGCAGAGCCACAGGAGTCCCCAGAGGGAGGGC
GGGAACAGAAAATGTTCCCGATGGCGGCGGTCTGTTTTAT
mm4_exon_C230012O17Rik_chr4_24964047 ACACATGATGCTTTGTGATCCCCCCGCCCCCCCACTGGAGGGTCATCTCTCCGCAGCTCAATCTACTTTCATGGGGACTG
TGGCCATATTCTCCATCACTGCTCCTGTACATTTAGAAGCAGGCACACAACTGA
mm4_intergenic_Gm4922|Perp_chr10_18837333 AAGCACCCAATCCTCCAGAGGCCCTCTACCCCTCTAGACAGTCCCCTTCCCGTCCCTCCCAGCTTCCTCTTCTACATGGA
AGAAAGGCACAGAAGCAGCAGAGCAGTGACCTGCGTGAAACCAAC
mm4_intron_Pde4b_chr4_102504118 CAGATCCTTAGGCCCTGCACCAGACATATGAAAACGAACTCTTCAGAGGTGGGAAAGGTATTTATGAAATATCTCCCAGA
GTGAGGCTGATGCTTGCTT
mm4_intergenic_Appl2|1500009L16Rik_chr10_83695392 ACAAAACCAAAGGCTCAGAGAGGACTCTCAAGACTGGGGGGAGGAACAGGATCACCTGCTGCACTGTTCTGACT
mm4_intron_Rgs22_chr15_36138445 GCAGATGTGGGGATGGCATATGGACTTGGGATTCTCAAGAGATGGGGAAGGGTAGAGCTACAGGGGCGAAGGAAGAGCTC
ACCCACAAACTTCTGGAGAGCAGAGTCCCAAAGATCAATACCTCACAGGAGTGGGGAG
mm4_exon_Map3k3_chr11_106123792 AGCTCACACTTTTGCCTCCTCTCTTATAGACTAGTTCCTCTCCCCACTCTGGAGTGTCCAGGCAGGTTCGGATCAAGCCT
TCCCAGTCTGC
mm3_intergenic_Cmtm6|Cmtm7_chr9_114750403 TCGCTAATGCCCACCCATTTTCTGCTGAGACTTCTGTCCACAGAGTTGAGACTTTACAGTCACAGGACTGTCCAGAGGTG
AGGAAGGGTTGTTGATTCTCCAGAGTTCCCCATGAGC
mm4_intron_Map4_chr9_109932036 CACTAACCTCCACGACTCCCGCCCAGGTTCTCGCGGTGGCCGCCGGGGACCCCGCAGGTCCCTCCCGAGGCGGGGAAGGG
CGGTGGCTGTCCGCCTACGGGACGCCGGGGTGGTTGAGAGAGGGG
mm4_intron_Gm16722_chr3_30038826 TTCACCAGCATTGAAGGACCCCATCTCCCCGACTGCAGAGTCCCTTTTAATTCCACTTTCTGATACTTAGCTAACTTAAT
GGCTTTCCAGTATGATCACCTCTTTCTTAAAAGTTATACTTTAGAACCCCAACTGCCA
mm4_intergenic_Gm13061|Zfp683_chr4_134035944 AATTGGACAGTGGTCGCTGGAGCTTGGGCCAAAGCAGTCTGCAGAGTGGGGGAGGGTGGGGATAAAAGCCAAACAATTGG
AAGTCAGGACTAGATAGATACCGAGGTTGGGAGGAGAG
mm2_intergenic_Fam174a|Gm15427_chr1_95449331 ACGTAGGCTAATTAGAAACATGCTAGAAAATCAGCTCTCCCCCACTCTGGAGAGTTCACAGGCATTTCTGCAAACACACT
CACTAAAATCAGCATCCTTAACTTCTCTGCTGCAATGGCTCA
mm4_intergenic_Eftud1|4933406J10Rik_chr7_82725045 ACGCTGTTTTAGTATGCTGCCTGCCTTTCCCACCTCTGAAGAGTACCAGAGGCCCACAGAAGGTACCTGGCTG
mm4_intron_Cacna1g_chr11_94439244 ACACCCTCCTTACATCCTCCAAGGACTTGTACTGAGGTGAGGTGTCTGTGGGGTCTCCAGTGGGGGCGAAGGGCAATGAG
CGAAGGGCAAAA
mm4_intergenic_Als2cl|Gm590_chr9_110901058 TAAAGGGTACGGGCTGTGTGCCATGCCCAGGGACACTCCAGAGAGGTCGAAGGGTCTGCAAAGGCTGTTTGCCAGGACTG
GGTAGGGTTAAGCTGGGCATCTAGTGTCC
mm4_intergenic_Gm9025|Gm15322_chr13_111982949 GAGAGAAGACAGCAGCTGGCCTGTCAGGGCTCTCCAGTGGGGAGGCAGGAAGCAGTATTTGCATCTAAACTGCTGTGACC
CATCACAAAAAAATTAGAGCCCCTACTTCTAGGTTCCTGAGCACCTACCAT
mm4_intergenic_Trhr2|Gm20681_chr8_122363270 GTCCCAGGGCCAATCTGTACCAACATCAGCAGCCTAGGTCTCTCCAGCAGGGGGGCGGGGAGGGGAAGCATGGACACTGT
CCTCTTGGGCTTG
mm4_intergenic_Gm6594|Pkdcc_chr17_82946322 GCCTGTTAAAGCACTGTGCCTCAAACTAGCCTGAAGCTTGTTCTGCCCTGTGGGGACGGCCCAGAGGTGGGGATGGTGTT
TAGGGCACCAGGGACACTGGATCCTTCTCCTTGGCTCTCCCAC
mm4_intron_Spock1_chr13_57543734 ACAGACGAGGGAGCCTACTTCCATGGCATAACTATCGTCCCCCCCTCTGAAGGGCTCCATGGCCTCTGGACAGCACCGCA
GCTGAGGCTCAGGCCGTCAGCACTGCCCACTGGAACACATT
mm4_intron_Pitx1_chr13_55833964 GGTTCACTGAAGCAGGTCGAGTTCTATTCCACTACCCTTCCCACCTGTGCAGAGTCCGAAATATTTCTCTGTCCTTCAAG
AAACCGGGAGACGGTGATCTGGAGATGCCTGCATTGTTGTGGG
mm4_intergenic_Fbxo16|Zfp395_chr14_65345441 ACGGACATGAGAAATTTAGAGACTAAATCATTACAAATAGGTTTAAACTTGGGCTCTCCAGAGCTGGGAATGGGTCTAGT
TCCATATTAGAGCTCTTGACCAGTGTGC
mm4_intergenic_4930569F06Rik|Suds3_chr5_116827851 CCAACCAGCACATTTGGCTGCTCCTCAGTAGCCCCCAGCTTCTGCCTCGCTTCCATCCCCAACCCTGGAGAATCCATTCC
TCTTATGGCTTCTGCCCTCAATTCTGC
mm4_intergenic_Gm24070|Gm24994_chr12_77906180 GGGAGAGAACAGGAGGCCTAAAGTCTACTTCAGGAGTAGAGAACGGTATGTTTCCCCCTCCCCAGCTCTGCAGTGTCCCC
GCTCCCTCTTAGCTAATCA
mm4_intron_Pax7_chr4_139754747 AATAAGGGGCCCAGCTTCACCCACCCCCTTCCGCCCCTGGGGAGAGGCCTGCTGCTTTCAGGGGTGGCCCCCCTCCCTTC
CACAGCCTCTGCGCTGCAACCTCTCCTCTGTGG
mm4_intron_Ttll6_chr11_96146343 AAGCTTTCCCAGAAGCTGCTGGCACGCCACAAATGTCACACTGCTCCTCTGAACAACATGGACTCTTCAGGGGGCGGGCG
GGAACTGGCCTTCTAGCTGCCTCCTCT
mm4_intron_Tmem40_chr6_115742045 TTCCTTCAACAGCAGGCACATGAGACCCTTCCCACCTTCTGGAGACTCTCCCCACTTCCAGCACAAGCTGTCCAGAAACT
TCTTGAGATCTGGGGAGTGGCCAAGGACCTGCCCGACTAGGAAGTGTG
mm4_intron_Parva_chr7_112557587 CCTAGCTCTCACACCCTCCTCCTCCCTCCCTCCCAACCTCTGCAGAGACCGGAGTCGGTCACCAGCAGTCTAG
mm4_intron_Lrfn2_chr17_48948457 GGCAGTACAAAGTGGAGGCTCAGCTGCCAGGGACAAGGGGACTCTGCTGAGGGTGGAATGGTCCATGCAAGATGTGTGAA
GTCCAGATCTAGGAAGGAAGAGGCTCTTGAGCTCAGCATCCCTCCATC
mm4_intron_Lpin1_chr12_16543399 TGAGCAGTGGGGTTTGACAGGCTCGGAGGCTCCTGAGGGTGGGAAGGAAGCACTGGGCTATGTGCTCAGAGAAGCCAGCT
CCGGAGCAAGTGTGTATGCGTGC
mm4_intron_Ifltd1_chr6_145550135 TGTCTTTTAATCTCCCAGTGTCTCCCTCCCACCCTCAGCAGACTCCCTTTGCCTGCTGCTTTCTTAGGAGATCAGTCTGA
AGAGAAATGTACAAGCAAGCTAACAGTTTTTGAGTCCCAAAGAGCCATGG
mm4_intron_A230108P19Rik_chr2_6300007 CTTCCTCCCCTCCCAGGTAGCCTCTCTCCCTCTGGACTCTCCTGGGGGTGAGAGGGCTGCCCCTTCCCAGTGTTTTTTAT
TCCAGTGCGGCACATCCCAAAGTAT
mm4_intergenic_Zfp957|Rgcc_chr14_79281620 GAGTCCCTCGGTCATGGTTCCTGAGGAAGCAACATGTGCTGGGACCCTCCAGAGGCTGGGTTGGCACATCATACTTGAGC
TGGCACTAAATCGTCT
mm4_intergenic_Scgb1b27|Gm12760_chr7_34023936 GCCAAGTCAGTTTGTGGCTGTCTCCAGCATTCACCTAGTCTATAGCATGTGTCCCTTCCCACACACTCGAGAGTCCTATT
CACCTTTCCAGTTGTCTCATGCTTCTCCATCACATCATATCTTGAGACCCTATGACGGG
mm4_intergenic_Napa|Kptn_chr7_16118779 ACCTCAGTCCAGATCCTGCTCTCCCTCAGCGTCGGGCTCAAACTTTGTAAAGCTCTTTGCATAGTTCTGGTGAGAAGTTT
GTGAGGGACTCTCTAAAGGGTGTGATGGCACCAGGAGCAGTGTGAGTGGACAA
mm4_intergenic_Igfbp5|Tnp1_chr1_72877286 AGTCCATTCTCTCTGCCCTCTATTCGAAAAGCCCTGGACTCTTCAGAAGATGGGAGGGGGGGGGACTTAATATCAACAGA
GTTTCACTGAGATGACTAACTTATTCTGGACACACTGAAATCTGGTCGCACTCTCAGAGA
mm4_intergenic_Gm8682|Map3k15_chrX_160036962 CTCCTTTCCTGTCAGGCTGGAACGGCTGGGGGGATCCCAGCACGGGGAGTGCTATCCCTTCACCCCAGTTGTGAAACATC
CCTCCCACCCCCTGGACTGTCCTTCTCCCTCCATCCCTAAAGGTTCAGGCCTTCCCAAAC
mm4_intergenic_Gm6180|Gm23531_chr8_42247557 CTTTCCTGCCAGACCTGAGGGGCTGGGGGGATCCCAGCAGGGGGAGGGCTATCCCTTCACCCCAGTTGCCAAACATCCCT
CCCACCCCCTGGACCGTCCTTCCCCCTCCATCCCTGACTGTTCTGGCCTTCCCAA
mm4_intergenic_Gm23293|Gm11202_chr11_79600556 TCCCCTCTCACAGTGATCCCTTTTGCTGGTGTAACCTGATGAGCCTGGACCCTGGCGGGATGGAGTCCCTGGGTGCAGCA
CTCCCCAGAGGGTGGAAGGGTCCTGTGTCTGGAACCGAAGCCCTAAGG
mm4_intergenic_Glb1|Ccr4_chr9_114476589 TGTGAGGGATCTAAGCTGTGGTACAATACCACATGTGGGCTATAGGAACGTAAAGTGACTGTCCAGAGAGTGGGAGGGGA
CACGCTGGCTCTAGGCAACTGTGT
mm4_intergenic_Adra1b|Gm12153_chr11_43915272 AGCACAGGGTTCCTTGGAAGGAAACTGGTGTGTGTGTGTCATGTGACTGGAGTCTCCTGAGGGTGTGAAGGTGATCCCCT
AGTGCAGCTCAGCTCAGTTTGCCCCAGTTCCTACAGGCTGCAGAGACT
mm4_intergenic_Adam34|Gm24591_chr8_43817293 GTGTGCACTGTCCCTACTGGCATCCACCCCACCCACTGGTGAGTCCTGAGTCTATATGGTGGTAGTCCCCAAACC
mm4_exon_Prdm14_chr1_13125808 GCATGCGCGTAGTAGGTAGTCAGGTGGTGAGGGTTGCTCTGGGGCAGGTAGTTCGCCTTGTCCTGGGACTGGGTCTCACC
AGAGGGCGGTAAGGCCATCGCAAGCTGAGTTTTGCGGATCTGTGGGAAACCGAGCTG
mm4_exon_Cfl1_chr19_5493639 TCACCCCAGTTGCCAAACATCCCTCCCACCCCCTGGACCGTCCTTCTCCCTCCATCCCTGACGGTTCTG
mm3_intergenic_RP24-234J3.4/Gm21971|Gm21971_chr12_102726024 CGGCAGCCAGTTCACTTAGAAGGTCTGGGCTGTAGCTTATGGGACTCTCCAGAGGGTGGAGGGGAGAGTGCTGGGAAATG
CATATGGTGCTCTCAGGGAGGGGTTCCTGCTTGGTAG
mm3_intergenic_Onecut1/Gm20649|Fam214a_chr9_74907024 CTCTGAGGTCTGTGTGGCTGGAGGAACTAAAGACTCTAGAGAGAGACTTCAACTGCCCTCCCACCCTCGGGTGAGTCCTC
TGATAATATTAACACAGAGCTTCCTGCTACCTCTGAAGTGGGTCCCTGAACTTGG
mm3_intergenic_Gm23558|Galc_chr12_97676888 AGCAGTTTGGGGAAGCTAGAACCATCAAGGATAAAGAGAGGAGGAGTCTCCAGAGGGTGGGGGGGATGTTTGATAATTGG
GGTGAAGGAATTATCCTCTCCCTGATGGGATCCCTCTAGCCTCTC
mm3_intergenic_Gm14694|Gm6897_chrX_74854864 TTGCTGTGACCCTGCAGAATCCATCCAGCCCTACTCAGCCTATGGACCAAACCACCCTCTGGAGAGTCCAACTAGGACTG
TTCAAAATGCCCTCTGAGTCCTGAGGAACTGTATTGTCCAGAGTCAGGCAGACAC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.