CRISPOR

DNA break repair outcome predictions

Guide: CCGTGGGGCACCATCTTCTC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGGCTCGCGGGCTCAGGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTCACGGCTTCGGACAAA`	(Wild-type sequence)
283	`GCTCAGGCAACCGTGGGGC---------------GGTTCAGGCAGCGGGCTGCTCACGGC`	`15 bp deleted → no frameshift`
230	`GCTCAGGCAACCGTGGGGCACCATCTTC-----------AGGCAGCGGGCTGCTCACGGC`	`11 bp deleted → frameshift`
213	`GCTCAGGCAACCGTGG-----------------CGGTTCAGGCAGCGGGCTGCTCACGGC`	`17 bp deleted → frameshift`
210	`GCTCAGGCAACCGTGGGGCA------------------------GCGGGCTGCTCACGGC`	`24 bp deleted → no frameshift`
191	`GCTCAGGCAACCGTGGGGCACCATCTTCTC---------AGGCAGCGGGCTGCTCACGGC`	`9 bp deleted → no frameshift`
191	`GCTCAGGCA-----------------------------------GCGGGCTGCTCACGGC`	`35 bp deleted → frameshift`
179	`GCTCAGGCAACCGTGGG----------------CGGTTCAGGCAGCGGGCTGCTCACGGC`	`16 bp deleted → frameshift`
169	`GCTCAGGCAACCGTGGGGC-------------------------------TGCTCACGGC`	`31 bp deleted → frameshift`
163	`GCTCAGGC--------------------------GGTTCAGGCAGCGGGCTGCTCACGGC`	`26 bp deleted → frameshift`
162	`GCTCAGGCAACCGTGGGG------------------TTCAGGCAGCGGGCTGCTCACGGC`	`18 bp deleted → no frameshift`
154	`GCTCAGGCAACCGTGGG-------------------TTCAGGCAGCGGGCTGCTCACGGC`	`19 bp deleted → frameshift`
149	`GCTCAGGCAACCGTGGGGCACCATC--------------AGGCAGCGGGCTGCTCACGGC`	`14 bp deleted → frameshift`
147	`GCTCAGGCAACCGTGG--------------------TTCAGGCAGCGGGCTGCTCACGGC`	`20 bp deleted → frameshift`
143	`GCTCAGGCAACCGTGGGGCACCATCTTCTC-------------------------ACGGC`	`25 bp deleted → frameshift`
133	`GCTCAGGCAACCG----------------------GTTCAGGCAGCGGGCTGCTCACGGC`	`22 bp deleted → frameshift`
128	`GCTCAGGCAACCGTGGGGCACCA-----------------GGCAGCGGGCTGCTCACGGC`	`17 bp deleted → frameshift`
121	`GCTCAGGCAACCGTGGG--------------------------------CTGCTCACGGC`	`32 bp deleted → frameshift`
114	`GCTCAGGCAACCGTGGG-------------------------CAGCGGGCTGCTCACGGC`	`25 bp deleted → frameshift`
110	`GCTCAGGCAACCGTGGGGCA--------------------GGCAGCGGGCTGCTCACGGC`	`20 bp deleted → frameshift`
109	`GCTCAGGCAACCGTGG--------------------------CAGCGGGCTGCTCACGGC`	`26 bp deleted → frameshift`
105	`GCTCAGGCAACCGTGGGGCACCA---------------------GCGGGCTGCTCACGGC`	`21 bp deleted → no frameshift`
103	`GCTCAGGCAACCGTGGGGC---------------------------GGGCTGCTCACGGC`	`27 bp deleted → no frameshift`
99	`GCTCAGGCAACCGTGGGGCACCATCTTCT----------------------GCTCACGGC`	`22 bp deleted → frameshift`
95	`GCTCAGGCAACCGT-----------------------TCAGGCAGCGGGCTGCTCACGGC`	`23 bp deleted → frameshift`
94	`GCTCAGG-----------------------------TTCAGGCAGCGGGCTGCTCACGGC`	`29 bp deleted → frameshift`
89	`GCTCAGGCAACCGTGGGG------------------------------GCTGCTCACGGC`	`30 bp deleted → no frameshift`
86	`GCTCAGGCAACCGTGGGGCACCATCT-------------------------GCTCACGGC`	`25 bp deleted → frameshift`
82	`GCTCAGGCAACCGTGGGGCAC------------------------------------GGC`	`36 bp deleted → no frameshift`
80	`GC--------------------------------GGTTCAGGCAGCGGGCTGCTCACGGC`	`32 bp deleted → frameshift`
77	`GCTCAGGCAACCGTGGGGCACCATCTTC---------------------------ACGGC`	`27 bp deleted → no frameshift`
77	`GCTCAGGCAACCGTGGGGC-----------------------------------------`	`41 bp deleted → frameshift`
76	`GCTCAGGCAACCGTGG---------------------------------CTGCTCACGGC`	`33 bp deleted → no frameshift`
74	`GCTCAGGCAACCGTGGGGCACCATCT----------------------------CACGGC`	`28 bp deleted → frameshift`
73	`GCTCAGGCAACCGTGGGGC----------------------------------TCACGGC`	`34 bp deleted → frameshift`
73	`GCTCAGGCAACCG----------------------------------GGCTGCTCACGGC`	`34 bp deleted → frameshift`
73	`GCTCAGGC------------------------------------------TGCTCACGGC`	`42 bp deleted → no frameshift`
71	`GCTCAG---------------------------------------CGGGCTGCTCACGGC`	`39 bp deleted → no frameshift`
66	`GCTCAGGCAACCGTGGGGCACCATC------------------------------ACGGC`	`30 bp deleted → no frameshift`
63	`GCTCAGGCA-------------------------------GGCAGCGGGCTGCTCACGGC`	`31 bp deleted → frameshift`
62	`GCTCA---------------------------------------------------CGGC`	`51 bp deleted → no frameshift`
60	`GCTCAGGC--------------------------------------GGGCTGCTCACGGC`	`38 bp deleted → frameshift`
57	`GCTCAGGCAACCGTGGGGCACCA---------------------------------CGGC`	`33 bp deleted → no frameshift`
51	`GCTCAGG-----------------------------------------GCTGCTCACGGC`	`41 bp deleted → frameshift`
51	`GC-----------------------------------------AGCGGGCTGCTCACGGC`	`41 bp deleted → frameshift`
48	`GCTCAGGCAACCGTGGG------------------------------------------C`	`42 bp deleted → no frameshift`
47	`GCTCAGGCAACCGTG-------------------------------------CTCACGGC`	`37 bp deleted → frameshift`
46	`GCTCAGGCAACCGTGG-------------------------------------------C`	`43 bp deleted → frameshift`
45	`GCT------------------------------------------------GCTCACGGC`	`48 bp deleted → no frameshift`
44	`GCTCAGGC----------------------------------------------------`	`52 bp deleted → frameshift`
44	`GC--------------------------------------------GGGCTGCTCACGGC`	`44 bp deleted → frameshift`
42	`GCTCAGGCAACCG---------------------------------------------GC`	`45 bp deleted → no frameshift`
42	`GCTCAGGC---------------------------------------------TCACGGC`	`45 bp deleted → no frameshift`
30	`GCTCAGGCAAC----------------------------------------------GGC`	`46 bp deleted → frameshift`
28	`GCTCAGGCA-----------------------------------------------CGGC`	`47 bp deleted → frameshift`
22	`GC----------------------------------------------------------`	`58 bp deleted → frameshift`

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