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Contents:

PCR primers for off-targets of CTCTTCTCAGCCAAGATCCC AGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313759_F TCGTCGGCAGCGTCCTGCCTTGTCGCCTTAGCT 60.0 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313759_R GTCTCGTGGGCTCGGGTCCTTTCGGCTCTCCATCC 60.1 1.00
mm2_intergenic_Gm11442|Appbp2/D630032N06Rik_chr11_85224732_F Primer3: not found at this Tm N.d. 0.81
mm2_intergenic_Gm11442|Appbp2/D630032N06Rik_chr11_85224732_R Primer3: not found at this Tm N.d. 0.81
mm3_intergenic_Nup93|Mir138-2_chr8_94315245_F TCGTCGGCAGCGTCGTGCTGCTGTTCTCTGGAGT 59.9 0.58
mm3_intergenic_Nup93|Mir138-2_chr8_94315245_R GTCTCGTGGGCTCGGCCGTCTCTGGAACGTACACC 60.1 0.58
mm4_intergenic_n-R5s193|Spsb1_chr4_149856420_F TCGTCGGCAGCGTCCGGTGACTTGCAGCTGACTA 60.0 0.57
mm4_intergenic_n-R5s193|Spsb1_chr4_149856420_R GTCTCGTGGGCTCGGAGACACTGGCTCTGAGACCT 59.8 0.57
mm3_intergenic_Gm25818|Creb5_chr6_53435031_F TCGTCGGCAGCGTCAATGCAGTTCCGAACCACCT 59.8 0.56
mm3_intergenic_Gm25818|Creb5_chr6_53435031_R GTCTCGTGGGCTCGGAAGCCATCACCAGACTGAGC 60.0 0.56
mm4_intergenic_Ccdc178|Asxl3_chr18_22183122_F Primer3: not found at this Tm N.d. 0.54
mm4_intergenic_Ccdc178|Asxl3_chr18_22183122_R Primer3: not found at this Tm N.d. 0.54
mm3_intergenic_Sema3e|Pclo_chr5_14386651_F TCGTCGGCAGCGTCTGTTACGGATTTCCAACCGC 58.8 0.51
mm3_intergenic_Sema3e|Pclo_chr5_14386651_R GTCTCGTGGGCTCGGGCTAGCAGCTATGACCCTGG 59.9 0.51
mm4_intergenic_Ubap2|Dcaf12_chr4_41289867_F Primer3: not found at this Tm N.d. 0.49
mm4_intergenic_Ubap2|Dcaf12_chr4_41289867_R Primer3: not found at this Tm N.d. 0.49
mm4_intron_1700112E06Rik_chr14_22683689_F Primer3: not found at this Tm N.d. 0.41
mm4_intron_1700112E06Rik_chr14_22683689_R Primer3: not found at this Tm N.d. 0.41
mm3_intergenic_Gm5046|Gm23907_chr15_65313530_F TCGTCGGCAGCGTCCTTTGCATGTGTGGAAGGGC 60.0 0.37
mm3_intergenic_Gm5046|Gm23907_chr15_65313530_R GTCTCGTGGGCTCGGACAGTACTTTGATTCGTTGTGCA 59.1 0.37
mm4_intergenic_Gm24774|Pcdh17_chr14_83589061_F Primer3: not found at this Tm N.d. 0.36
mm4_intergenic_Gm24774|Pcdh17_chr14_83589061_R Primer3: not found at this Tm N.d. 0.36
mm4_intron_Piezo2_chr18_63217413_F TCGTCGGCAGCGTCCTGAGGGAAAGAGCCAGTGG 60.0 0.33
mm4_intron_Piezo2_chr18_63217413_R GTCTCGTGGGCTCGGTGCATTTCCAGCAAACTGCC 59.9 0.33
mm4_intergenic_Gm16056|Dpp6_chr5_26891469_F Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Gm16056|Dpp6_chr5_26891469_R Primer3: not found at this Tm N.d. 0.32
mm4_intergenic_Edn2|Foxo6_chr4_120182107_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Edn2|Foxo6_chr4_120182107_R Primer3: not found at this Tm N.d. 0.30
mm3_intron_Agk_chr6_40347787_F TCGTCGGCAGCGTCACCCTGAATCTGGAATAGCCC 59.2 0.30
mm3_intron_Agk_chr6_40347787_R GTCTCGTGGGCTCGGGGCAGTGCAGTGCTAGGAAT 60.3 0.30
mm4_exon_Lrrc4_chr6_28831642_F TCGTCGGCAGCGTCTGCACAGTTACCTGCCACAA 60.1 0.28
mm4_exon_Lrrc4_chr6_28831642_R GTCTCGTGGGCTCGGCTAGCTCACTTAAGCCGGGG 59.8 0.28
mm4_intron_Gpr98_chr13_81332013_F Primer3: not found at this Tm N.d. 0.28
mm4_intron_Gpr98_chr13_81332013_R Primer3: not found at this Tm N.d. 0.28
mm4_intron_Nrxn2_chr19_6511468_F TCGTCGGCAGCGTCGCTAGGAGGAAGGCATGGTG 60.1 0.28
mm4_intron_Nrxn2_chr19_6511468_R GTCTCGTGGGCTCGGCCTACCTCCAACACCCCAAA 59.2 0.28
mm4_intergenic_Ncald|Gm23654_chr15_37369084_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Ncald|Gm23654_chr15_37369084_R Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_Cpeb2|C1qtnf7_chr5_43435525_F TCGTCGGCAGCGTCCCCATATTTCATGACAGCCAACC 59.9 0.26
mm4_intergenic_Cpeb2|C1qtnf7_chr5_43435525_R GTCTCGTGGGCTCGGAGCACAGTGTCTGGAAGCAA 59.8 0.26
mm4_intron_Rps6ka1_chr4_133864534_F TCGTCGGCAGCGTCTGGACTGGACCTTAGCTGGT 60.1 0.25
mm4_intron_Rps6ka1_chr4_133864534_R GTCTCGTGGGCTCGGAGACACTGATTCTGCCCTGG 59.3 0.25
mm4_intron_Pop1_chr15_34496774_F Primer3: not found at this Tm N.d. 0.25
mm4_intron_Pop1_chr15_34496774_R Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Gm6462|Gm25586_chr1_29224826_F Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Gm6462|Gm25586_chr1_29224826_R Primer3: not found at this Tm N.d. 0.25
mm4_intron_Agap1_chr1_89627498_F Primer3: not found at this Tm N.d. 0.25
mm4_intron_Agap1_chr1_89627498_R Primer3: not found at this Tm N.d. 0.25
mm4_intergenic_Itm2b|Med4_chr14_73488926_F TCGTCGGCAGCGTCATGAGGTGGCCAGCTGAAAA 59.8 0.25
mm4_intergenic_Itm2b|Med4_chr14_73488926_R GTCTCGTGGGCTCGGCCTCAAGCTCAGGACTCTGC 60.1 0.25
mm4_exon_Smg1_chr7_118243253_F Primer3: not found at this Tm N.d. 0.24
mm4_exon_Smg1_chr7_118243253_R Primer3: not found at this Tm N.d. 0.24
mm4_intron_Glrb_chr3_80911353_F Primer3: not found at this Tm N.d. 0.24
mm4_intron_Glrb_chr3_80911353_R Primer3: not found at this Tm N.d. 0.24
mm4_exon_Rnf165_chr18_77462977_F TCGTCGGCAGCGTCTCCCCTTCATCTTTCTTGCCC 59.9 0.23
mm4_exon_Rnf165_chr18_77462977_R GTCTCGTGGGCTCGGCAGTTGCCAGCAGGAGTCTC 60.6 0.23
mm3_intron_Ndrg1_chr15_66961966_F Primer3: not found at this Tm N.d. 0.23
mm3_intron_Ndrg1_chr15_66961966_R Primer3: not found at this Tm N.d. 0.23
mm3_intron_Arhgap15_chr2_44287005_F TCGTCGGCAGCGTCTCAAGCAACTCTCCAGTGGT 58.8 0.23
mm3_intron_Arhgap15_chr2_44287005_R GTCTCGTGGGCTCGGGTACTCTCAGTTACCTGCCCC 59.5 0.23
mm3_intergenic_Nfic|Celf5/Gm16105_chr10_81455932_F Primer3: not found at this Tm N.d. 0.22
mm3_intergenic_Nfic|Celf5/Gm16105_chr10_81455932_R Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Helt|Acsl1_chr8_46374175_F Primer3: not found at this Tm N.d. 0.22
mm4_intergenic_Helt|Acsl1_chr8_46374175_R Primer3: not found at this Tm N.d. 0.22
mm4_intron_Tnni1_chr1_135798567_F TCGTCGGCAGCGTCCTGAGAGCCTGCTTCTGACC 60.1 0.21
mm4_intron_Tnni1_chr1_135798567_R GTCTCGTGGGCTCGGTCAGAGAAAGACAATGCCTGCT 59.9 0.21
mm4_exon_Flnc_chr6_29454413_F TCGTCGGCAGCGTCCACCCGGAGACTACAGCATC 59.8 0.21
mm4_exon_Flnc_chr6_29454413_R GTCTCGTGGGCTCGGAACTGACTCGAGCATGGGTG 60.0 0.21
mm4_intergenic_AC154801.1|BC040587_chr16_40742806_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_AC154801.1|BC040587_chr16_40742806_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm25658|Atp2b1_chr10_98700062_F Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm25658|Atp2b1_chr10_98700062_R Primer3: not found at this Tm N.d. 0.19
mm4_exon_Flnb_chr14_7927497_F TCGTCGGCAGCGTCGCCAGGGGACTACAGCATTC 60.4 0.19
mm4_exon_Flnb_chr14_7927497_R GTCTCGTGGGCTCGGAGGCACAGCTAGATCTCCCA 60.0 0.19
mm4_intron_Gm11213_chr4_63599652_F Primer3: not found at this Tm N.d. 0.19
mm4_intron_Gm11213_chr4_63599652_R Primer3: not found at this Tm N.d. 0.19
mm4_intergenic_Gm8054|Gm22044_chr8_78940820_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm8054|Gm22044_chr8_78940820_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Plscr5|Plscr1_chr9_92228995_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Plscr5|Plscr1_chr9_92228995_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Iars|Gm7251_chr13_49749694_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Iars|Gm7251_chr13_49749694_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm13668|A630050E04Rik_chr2_74084756_F TCGTCGGCAGCGTCGAGACTGTGGCCAAGGACTC 60.0 0.17
mm4_intergenic_Gm13668|A630050E04Rik_chr2_74084756_R GTCTCGTGGGCTCGGTCTTCTGCCAATAAGTGCTCTGA 59.7 0.17
mm4_exon_Adamts14_chr10_61198415_F TCGTCGGCAGCGTCGGAACCTGTGCTACCTCCTG 59.7 0.17
mm4_exon_Adamts14_chr10_61198415_R GTCTCGTGGGCTCGGGTGACATGAGCCCATCCCTC 60.1 0.17
mm4_intergenic_Gm13994|Slc24a5_chr2_125046183_F TCGTCGGCAGCGTCCAGTGGGCATTGCTGGAAAA 59.3 0.16
mm4_intergenic_Gm13994|Slc24a5_chr2_125046183_R GTCTCGTGGGCTCGGTCCCCTCTAAGTGGTGGGAA 59.5 0.16
mm4_intron_Chchd3_chr6_32871101_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Chchd3_chr6_32871101_R Primer3: not found at this Tm N.d. 0.16
mm4_exon_Acsl6_chr11_54315334_F Primer3: not found at this Tm N.d. 0.16
mm4_exon_Acsl6_chr11_54315334_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm21308|Gm21209_chrY_49559374_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm21308|Gm21209_chrY_49559374_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm21118|Gm20888_chrY_70430075_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm21118|Gm20888_chrY_70430075_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm20804|Gm21518_chrY_62179077_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Gm20804|Gm21518_chrY_62179077_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Rdh10|Stau2_chr1_16173476_F TCGTCGGCAGCGTCCCAGGAAACCACCCTCTGTG 60.2 0.15
mm4_intergenic_Rdh10|Stau2_chr1_16173476_R GTCTCGTGGGCTCGGGTGTTGTGGAGTCCTGAGGG 59.9 0.15
mm4_intron_Abcg1_chr17_31079258_F Primer3: not found at this Tm N.d. 0.15
mm4_intron_Abcg1_chr17_31079258_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Dleu2|Gm26969_chr14_62137993_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Dleu2|Gm26969_chr14_62137993_R Primer3: not found at this Tm N.d. 0.15
mm4_intron_Klhl29_chr12_5273493_F TCGTCGGCAGCGTCTCCAAGTGATCACAGCTGCC 60.3 0.15
mm4_intron_Klhl29_chr12_5273493_R GTCTCGTGGGCTCGGTAGGAAACCCTGGATCCCGT 59.9 0.15
mm4_intergenic_Irx1|Rpl9-ps4_chr13_72447216_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Irx1|Rpl9-ps4_chr13_72447216_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Ccnd1|Tpcn2_chr7_145020987_F TCGTCGGCAGCGTCCTAATGCCCTCCTGGACCTG 59.5 0.15
mm4_intergenic_Ccnd1|Tpcn2_chr7_145020987_R GTCTCGTGGGCTCGGCTCAGCATGCGCATCTGTTC 59.9 0.15
mm4_intergenic_Tm2d3|Gm7551_chr7_65727656_F Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Tm2d3|Gm7551_chr7_65727656_R Primer3: not found at this Tm N.d. 0.14
mm4_intergenic_Gm25261|Gm831_chr4_131359776_F TCGTCGGCAGCGTCTGAGGGAGGAACAGGTTGGA 60.1 0.13
mm4_intergenic_Gm25261|Gm831_chr4_131359776_R GTCTCGTGGGCTCGGGGAGACAGAAAGGACCAGCC 60.0 0.13
mm4_intron_Rgs3_chr4_62619998_F TCGTCGGCAGCGTCCGGGTTATAGGCAGCTGAGG 59.9 0.12
mm4_intron_Rgs3_chr4_62619998_R GTCTCGTGGGCTCGGAAAACCAAGCAGCAACAGCT 59.1 0.12
mm4_intron_4933434I20Rik_chr8_83366596_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_4933434I20Rik_chr8_83366596_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm8521|Gm20918_chrY_4664065_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm8521|Gm20918_chrY_4664065_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21943|Gm21724_chrY_57693888_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21943|Gm21724_chrY_57693888_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21923|Gm20880_chrY_50084065_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21923|Gm20880_chrY_50084065_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21902|Gm21285_chrY_53284274_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21902|Gm21285_chrY_53284274_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21895|Gm20933_chrY_65881986_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21895|Gm20933_chrY_65881986_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21877|Gm21530_chrY_62703796_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21877|Gm21530_chrY_62703796_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21874|Gm20821_chrY_9607712_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21874|Gm20821_chrY_9607712_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21873|Gm21760_chrY_80121959_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21873|Gm21760_chrY_80121959_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21853|Gm21679_chrY_29487809_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21853|Gm21679_chrY_29487809_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21849|Gm21117_chrY_51113444_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21849|Gm21117_chrY_51113444_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21848|Gm20736_chrY_65365911_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21848|Gm20736_chrY_65365911_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21841|Gm20842_chrY_71680241_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21841|Gm20842_chrY_71680241_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21841|Gm20842_chrY_71457630_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21841|Gm20842_chrY_71457630_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21840|Gm21258_chrY_52768252_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21840|Gm21258_chrY_52768252_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21837|Gm21910_chrY_66288584_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21837|Gm21910_chrY_66288584_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21825|Gm21333_chrY_82213382_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21825|Gm21333_chrY_82213382_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21822|Gm21763_chrY_19243134_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21822|Gm21763_chrY_19243134_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21813|Gm21777_chrY_87539161_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21813|Gm21777_chrY_87539161_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21808|Gm20866_chrY_70946162_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21808|Gm20866_chrY_70946162_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21800|Gm21729_chrY_41514568_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21800|Gm21729_chrY_41514568_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21799|Gm21835_chrY_37271387_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21799|Gm21835_chrY_37271387_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21796|Gm21462_chrY_86590452_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21796|Gm21462_chrY_86590452_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21792|Gm21287_chrY_88522768_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21792|Gm21287_chrY_88522768_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21778|Gm21746_chrY_6145099_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21778|Gm21746_chrY_6145099_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21774|Gm21805_chrY_42392500_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21774|Gm21805_chrY_42392500_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21767|Gm20822_chrY_15340328_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21767|Gm20822_chrY_15340328_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21767|Gm20822_chrY_14530812_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21767|Gm20822_chrY_14530812_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21766|Gm20800_chrY_73181456_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21766|Gm20800_chrY_73181456_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21766|Gm20800_chrY_73126849_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21766|Gm20800_chrY_73126849_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21763|AC201846.1_chrY_20052850_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21763|AC201846.1_chrY_20052850_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21760|Gm21257_chrY_80327240_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21760|Gm21257_chrY_80327240_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21757|Gm20846_chrY_72543061_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21757|Gm20846_chrY_72543061_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21752|Gm21477_chrY_87104992_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21752|Gm21477_chrY_87104992_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21749|Gm21302_chrY_53832145_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21749|Gm21302_chrY_53832145_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21722|Gm21282_chrY_81138975_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21722|Gm21282_chrY_81138975_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21722|Gm21282_chrY_80915129_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21722|Gm21282_chrY_80915129_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21720|Gm21838_chrY_89040915_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21720|Gm21838_chrY_89040915_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21717|Gm21095_chrY_84085449_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21717|Gm21095_chrY_84085449_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21634|Gm21654_chrY_63734720_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21634|Gm21654_chrY_63734720_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21617|Gm21634_chrY_63216758_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21617|Gm21634_chrY_63216758_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21477|Gm21813_chrY_87327843_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21477|Gm21813_chrY_87327843_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21476|Gm21506_chrY_27533721_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21476|Gm21506_chrY_27533721_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21443|Gm21755_chrY_26278894_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21443|Gm21755_chrY_26278894_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21427|Gm21890_chrY_25765750_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21427|Gm21890_chrY_25765750_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21412|Gm21773_chrY_25252701_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21412|Gm21773_chrY_25252701_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21396|Gm21245_chrY_51840283_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21396|Gm21245_chrY_51840283_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21330|Gm21858_chrY_55719934_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21330|Gm21858_chrY_55719934_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21241|Gm21739_chrY_44083295_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21241|Gm21739_chrY_44083295_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21198|Gm20808_chrY_78411361_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21198|Gm20808_chrY_78411361_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21198|Gm20808_chrY_78201117_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21198|Gm20808_chrY_78201117_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21065|Gm21871_chrY_22873048_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21065|Gm21871_chrY_22873048_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21065|Gm21871_chrY_22648947_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm21065|Gm21871_chrY_22648947_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20937|Gm21867_chrY_68306699_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20937|Gm21867_chrY_68306699_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20930|Gm20931_chrY_55201910_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20930|Gm20931_chrY_55201910_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20926|Gm21768_chrY_23710584_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20926|Gm21768_chrY_23710584_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20917|Gm20916_chrY_79307760_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20917|Gm20916_chrY_79307760_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20916|Gm21823_chrY_79530744_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20916|Gm21823_chrY_79530744_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20914|Gm21778_chrY_5854216_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20914|Gm21778_chrY_5854216_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20913|Gm21868_chrY_57175406_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20913|Gm21868_chrY_57175406_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20907|Gm20921_chrY_64461428_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20907|Gm20921_chrY_64461428_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20904|Gm21776_chrY_76220801_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20904|Gm21776_chrY_76220801_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20902|Gm21715_chrY_73657528_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20902|Gm21715_chrY_73657528_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20901|Gm21865_chrY_39718871_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20901|Gm21865_chrY_39718871_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20898|Gm21799_chrY_36747114_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20898|Gm21799_chrY_36747114_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20898|Gm21799_chrY_36448680_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20898|Gm21799_chrY_36448680_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20896|Gm21898_chrY_35933286_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20896|Gm21898_chrY_35933286_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20895|Gm20892_chrY_26788609_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20895|Gm20892_chrY_26788609_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20893|Gm21642_chrY_28283303_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20893|Gm21642_chrY_28283303_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20881|Gm20889_chrY_50596579_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20881|Gm20889_chrY_50596579_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20865|Gm21065_chrY_22063192_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20865|Gm21065_chrY_22063192_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20865|Gm21065_chrY_21838835_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20865|Gm21065_chrY_21838835_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20859|Gm21638_chrY_77307188_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20859|Gm21638_chrY_77307188_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20855|Gm21896_chrY_35143617_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20855|Gm21896_chrY_35143617_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20840|Gm21845_chrY_24226461_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20840|Gm21845_chrY_24226461_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20825|Gm20815_chrY_8728751_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20825|Gm20815_chrY_8728751_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20825|Gm20815_chrY_8463047_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20825|Gm20815_chrY_8463047_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20825|Gm20815_chrY_8246108_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20825|Gm20815_chrY_8246108_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20821|Gm21310_chrY_9980510_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20821|Gm21310_chrY_9980510_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20815|Gm21292_chrY_9073064_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20815|Gm21292_chrY_9073064_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20813|Gm21922_chrY_75184040_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20813|Gm21922_chrY_75184040_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20809|Gm21921_chrY_24739584_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20809|Gm21921_chrY_24739584_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20805|Gm21913_chrY_74458637_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm20805|Gm21913_chrY_74458637_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_AC201846.1|Gm20910_chrY_21005393_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_AC201846.1|Gm20910_chrY_21005393_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_AC201846.1|Gm20910_chrY_20862210_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_AC201846.1|Gm20910_chrY_20862210_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Dlg1_chr16_31782399_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Dlg1_chr16_31782399_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Spata13|C1qtnf9_chr14_60766680_F TCGTCGGCAGCGTCGGTAACTTGCCGCATCCTCT 60.1 0.12
mm4_intergenic_Spata13|C1qtnf9_chr14_60766680_R GTCTCGTGGGCTCGGAACCGAGGTTCCTTTCGGAC 59.9 0.12
mm4_intergenic_Gm22785|Gm8410_chrX_70693980_F TCGTCGGCAGCGTCTGACCACCAGGAGAAGACCA 60.1 0.11
mm4_intergenic_Gm22785|Gm8410_chrX_70693980_R GTCTCGTGGGCTCGGGGGTCTCTGAGTGTCTGGG 58.7 0.11
mm3_intron_Lrit3_chr3_129793870_F Primer3: not found at this Tm N.d. 0.11
mm3_intron_Lrit3_chr3_129793870_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm15867|Syt14_chr1_192891006_F TCGTCGGCAGCGTCGGTTAGATCTGACTCGTGGTGA 59.2 0.11
mm4_intergenic_Gm15867|Syt14_chr1_192891006_R GTCTCGTGGGCTCGGACACAATGGGCCAATGTCAC 59.0 0.11
mm4_intron_Rbfox3_chr11_118754506_F TCGTCGGCAGCGTCACAGGCCATGTGTGTGTCAA 60.1 0.10
mm4_intron_Rbfox3_chr11_118754506_R GTCTCGTGGGCTCGGCACACCATAGAGTGAGCCCC 59.8 0.10
mm4_intergenic_Mamld1|Gm14730_chrX_71188902_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Mamld1|Gm14730_chrX_71188902_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm25796|Gm23337_chr3_45858968_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm25796|Gm23337_chr3_45858968_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Mtch1_chr17_29339978_F TCGTCGGCAGCGTCCCTCTGACTGGGAAGGACCT 60.2 0.10
mm4_intron_Mtch1_chr17_29339978_R GTCTCGTGGGCTCGGGGTAAGAGCATGGCAGCTGA 60.1 0.10
mm4_intergenic_Mthfr-ps1|Gm25756_chr5_78515631_F Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Mthfr-ps1|Gm25756_chr5_78515631_R Primer3: not found at this Tm N.d. 0.10
mm4_intergenic_Gm23673|Gm9381_chr3_124934462_F TCGTCGGCAGCGTCCTCACTCCTACAGGGAAAACCA 59.3 0.09
mm4_intergenic_Gm23673|Gm9381_chr3_124934462_R GTCTCGTGGGCTCGGTCAACAGCCAGGGCCAATTT 60.4 0.09
mm4_intergenic_Ntsr1|Mrgbp_chr2_180577768_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Ntsr1|Mrgbp_chr2_180577768_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Epha3_chr16_63598510_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Epha3_chr16_63598510_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm11922|Map3k7_chr4_31627267_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm11922|Map3k7_chr4_31627267_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Plb1|Gm5864_chr5_32398748_F TCGTCGGCAGCGTCCCAGTGCCAATTTGTCCCCT 60.5 0.09
mm4_intergenic_Plb1|Gm5864_chr5_32398748_R GTCTCGTGGGCTCGGGGAGCCAGATGACCAAGGAC 60.1 0.09
mm4_intron_Mcf2l_chr8_13006930_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Mcf2l_chr8_13006930_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Tmem132d_chr5_128103070_F TCGTCGGCAGCGTCTCTACTCAGCTGGGGTCACT 59.5 0.09
mm4_intron_Tmem132d_chr5_128103070_R GTCTCGTGGGCTCGGGCAGAATTATCTCACCCGCCT 60.2 0.09
mm4_intergenic_Gm3104|Gm7353_chr7_3092054_F TCGTCGGCAGCGTCGCCTCACTTGAGTGGAGGAA 59.3 0.09
mm4_intergenic_Gm3104|Gm7353_chr7_3092054_R GTCTCGTGGGCTCGGACCATCTCTTTCCAGCTGGG 59.3 0.09
mm4_intergenic_Gm22011|Mthfr-ps1_chr5_78270634_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm22011|Mthfr-ps1_chr5_78270634_R Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Atp1b3|BC043934_chr9_96398824_F TCGTCGGCAGCGTCCAGAGAGGAAGGGAGGCCT 59.9 0.08
mm3_intergenic_Atp1b3|BC043934_chr9_96398824_R GTCTCGTGGGCTCGGAACACACCCTTCCCCATTCC 59.8 0.08
mm4_intergenic_Gm6117|Gm13847_chr6_31600110_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm6117|Gm13847_chr6_31600110_R Primer3: not found at this Tm N.d. 0.08
mm4_intron_Rcan2_chr17_43802399_F Primer3: not found at this Tm N.d. 0.08
mm4_intron_Rcan2_chr17_43802399_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_6030471H07Rik|Gm12416_chr4_84088417_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_6030471H07Rik|Gm12416_chr4_84088417_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm13677|Zfp804a_chr2_82229227_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm13677|Zfp804a_chr2_82229227_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Pgap1|Ankrd44_chr1_54561092_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Pgap1|Ankrd44_chr1_54561092_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Gm24394|Gm25204_chr14_36734642_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm24394|Gm25204_chr14_36734642_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Odf2l_chr3_145146101_F TCGTCGGCAGCGTCCTGCTGCTTGAGTCTCCTCA 59.3 0.07
mm4_intron_Odf2l_chr3_145146101_R GTCTCGTGGGCTCGGAAGGTGTCTGAGCTGAGCAC 59.9 0.07
mm4_intergenic_Dock10|Nyap2_chr1_80977703_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Dock10|Nyap2_chr1_80977703_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_D10Wsu102e|Aldh1l2_chr10_83485463_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_D10Wsu102e|Aldh1l2_chr10_83485463_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Cntnap5b_chr1_100479559_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Cntnap5b_chr1_100479559_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Fam155a|Gm10217_chr8_9814928_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Fam155a|Gm10217_chr8_9814928_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Tbx20|Gm23893_chr9_24876977_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Tbx20|Gm23893_chr9_24876977_R Primer3: not found at this Tm N.d. 0.07
mm4_exon_Atp6v1f_chr6_29469027_F Primer3: not found at this Tm N.d. 0.06
mm4_exon_Atp6v1f_chr6_29469027_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm23642|Lrfn2_chr17_48911944_F Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm23642|Lrfn2_chr17_48911944_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Gm24132|Gm25160_chr8_96728242_F TCGTCGGCAGCGTCAGGCCTACTAGATGGTTTCCA 57.8 0.06
mm4_intergenic_Gm24132|Gm25160_chr8_96728242_R GTCTCGTGGGCTCGGATTTTAGTGTCCCTGGCCCC 59.6 0.06
mm4_intron_Gabrg3_chr7_57027555_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Gabrg3_chr7_57027555_R Primer3: not found at this Tm N.d. 0.06
mm4_intergenic_Camk2n1|Vwa5b1_chr4_138516640_F TCGTCGGCAGCGTCGGTGAGATCATTGGTGGCCA 60.0 0.06
mm4_intergenic_Camk2n1|Vwa5b1_chr4_138516640_R GTCTCGTGGGCTCGGCATACTGGGCCACACCTCTG 60.1 0.06
mm4_exon_Reps2_chrX_162416457_F TCGTCGGCAGCGTCACAGCCAAGGATTGTTGCAG 59.0 0.06
mm4_exon_Reps2_chrX_162416457_R GTCTCGTGGGCTCGGAGCTTGGAAACCACCCAACA 60.0 0.06
mm4_intron_Kirrel_chr3_87097582_F Primer3: not found at this Tm N.d. 0.06
mm4_intron_Kirrel_chr3_87097582_R Primer3: not found at this Tm N.d. 0.06
mm4_intron_Sun1_chr5_139223404_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Sun1_chr5_139223404_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Postn_chr3_54364662_F TCGTCGGCAGCGTCTGTGCTTTCTGTGAACAAAACCT 59.4 0.05
mm4_intron_Postn_chr3_54364662_R GTCTCGTGGGCTCGGAAAGTCATGCTGGGGAAGGG 59.9 0.05
mm4_intron_Pkp4_chr2_59245364_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Pkp4_chr2_59245364_R Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Crisp4|Defb44-ps_chr1_18191408_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Crisp4|Defb44-ps_chr1_18191408_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Ulk4|Trak1_chr9_121302153_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Ulk4|Trak1_chr9_121302153_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Sall1|Gm6625_chr8_89076014_F TCGTCGGCAGCGTCGGGCGGCCATAGCATATCTA 59.1 0.05
mm4_intergenic_Sall1|Gm6625_chr8_89076014_R GTCTCGTGGGCTCGGTTGCCTCTCTGCTTCAAGGT 59.2 0.05
mm4_exon_Trim29_chr9_43311226_F Primer3: not found at this Tm N.d. 0.05
mm4_exon_Trim29_chr9_43311226_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Cntnap5b_chr1_100038514_F TCGTCGGCAGCGTCTGTGTGAACAAGTTTACCCAGTG 59.3 0.05
mm4_intron_Cntnap5b_chr1_100038514_R GTCTCGTGGGCTCGGATGCTTCCTCCACAGGCATT 59.6 0.05
mm4_intergenic_Gm26253|Rab1_chr11_20200928_F TCGTCGGCAGCGTCTTAGAGCAGCCTCAGACCCT 59.9 0.04
mm4_intergenic_Gm26253|Rab1_chr11_20200928_R GTCTCGTGGGCTCGGTGAGGAGGGGTGGAGACAG 59.9 0.04
mm4_intergenic_Zcchc16|Gm25097_chrX_145151586_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Zcchc16|Gm25097_chrX_145151586_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23892|Gm25382_chr10_16294078_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm23892|Gm25382_chr10_16294078_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm25621|Rpl32-ps_chr3_69692534_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm25621|Rpl32-ps_chr3_69692534_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Mkx|Armc4_chr18_7027090_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Mkx|Armc4_chr18_7027090_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm13812|Gm13810_chr2_100585198_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm13812|Gm13810_chr2_100585198_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm13810|B230118H07Rik_chr2_100728380_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm13810|B230118H07Rik_chr2_100728380_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ftmt|Srfbp1_chr18_52385904_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Ftmt|Srfbp1_chr18_52385904_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Unc5b|mmu-mir-6408_chr10_60859317_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Unc5b|mmu-mir-6408_chr10_60859317_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_4933413G19Rik|Nrip2_chr6_128393461_F TCGTCGGCAGCGTCCAGGGCAGTGTCCTCTCTTG 60.0 0.04
mm4_intergenic_4933413G19Rik|Nrip2_chr6_128393461_R GTCTCGTGGGCTCGGTCCGCTGAAGGCATGAATGA 59.7 0.04
mm4_intergenic_Gm16401/Prom1|Tapt1_chr5_44128377_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm16401/Prom1|Tapt1_chr5_44128377_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Prkd1_chr12_50639115_F TCGTCGGCAGCGTCTTTCCCACCTGTCCAAGTGG 59.8 0.04
mm4_intron_Prkd1_chr12_50639115_R GTCTCGTGGGCTCGGACCTCAGGCTTTTGGATTCCA 59.5 0.04
mm4_intron_Gm15545_chr7_44990572_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Gm15545_chr7_44990572_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Abca4_chr3_122139964_F TCGTCGGCAGCGTCGTCCTGGGTCAATCCTGCTC 60.1 0.03
mm4_intron_Abca4_chr3_122139964_R GTCTCGTGGGCTCGGTGTGCATGGAGGTCTGATGG 59.7 0.03
mm4_intergenic_AI467606|Qprt_chr7_127106971_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_AI467606|Qprt_chr7_127106971_R Primer3: not found at this Tm N.d. 0.03
mm4_exon_Ndrg3_chr2_156935364_F TCGTCGGCAGCGTCAATGGACGGATGGACAGACG 59.8 0.03
mm4_exon_Ndrg3_chr2_156935364_R GTCTCGTGGGCTCGGCAGGCCATCATCCCCGATTT 60.1 0.03
mm4_intergenic_Gm12126|Tenm2_chr11_36096384_F TCGTCGGCAGCGTCCTGGCCTCTCTCCAATTCCC 59.8 0.03
mm4_intergenic_Gm12126|Tenm2_chr11_36096384_R GTCTCGTGGGCTCGGAATAGCGAGGCGTTCTCTGC 60.5 0.03
mm4_intron_Ifi27_chr12_103434700_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Ifi27_chr12_103434700_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Calb2|Cmtr2_chr8_110168332_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Calb2|Cmtr2_chr8_110168332_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26166|Tsc22d2_chr3_58377384_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm26166|Tsc22d2_chr3_58377384_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Gm14055|Gm14064_chr2_137375075_F TCGTCGGCAGCGTCGGCTTTAGAACCCAGCTCCA 59.6 0.03
mm4_intergenic_Gm14055|Gm14064_chr2_137375075_R GTCTCGTGGGCTCGGTCTGGAAAGCCCCATCCCTA 59.9 0.03
mm4_intron_Prmt3_chr7_49824514_F TCGTCGGCAGCGTCCAACAGCCTTGAAGTCGCAC 60.0 0.03
mm4_intron_Prmt3_chr7_49824514_R GTCTCGTGGGCTCGGAAAGCCTTGTCCCCAACACA 60.0 0.03
mm4_intergenic_Sept6|Gm14542_chrX_37034370_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Sept6|Gm14542_chrX_37034370_R Primer3: not found at this Tm N.d. 0.03
mm4_intron_Alg5_chr3_54748240_F TCGTCGGCAGCGTCGCCTTCCAGGATAGGCTTCC 59.8 0.03
mm4_intron_Alg5_chr3_54748240_R GTCTCGTGGGCTCGGCACTCACAGCTGCTTCTCCA 59.9 0.03
mm4_intron_Kcnab1_chr3_65301674_F Primer3: not found at this Tm N.d. 0.03
mm4_intron_Kcnab1_chr3_65301674_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Cadm1|Nxpe2_chr9_47905177_F TCGTCGGCAGCGTCAATCCGTCCTGCCTTGAGTG 60.0 0.02
mm4_intergenic_Cadm1|Nxpe2_chr9_47905177_R GTCTCGTGGGCTCGGACAAGGTCTTGCTCCTGGTG 59.8 0.02
mm4_intron_Nt5c3_chr6_56904439_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Nt5c3_chr6_56904439_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Tenm3_chr8_48258150_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Tenm3_chr8_48258150_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Fras1_chr5_96680285_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Fras1_chr5_96680285_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Itpr2_chr6_146296726_F Primer3: not found at this Tm N.d. 0.02
mm4_exon_Itpr2_chr6_146296726_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Thrsp|Kctd14_chr7_97430100_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Thrsp|Kctd14_chr7_97430100_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_2310015A10Rik|Actn1_chr12_80137741_F TCGTCGGCAGCGTCCCCTTCCTCACGTCTTGTCC 60.0 0.02
mm4_intergenic_2310015A10Rik|Actn1_chr12_80137741_R GTCTCGTGGGCTCGGATTGAACGTCCCTCTGCTGG 60.0 0.02
mm4_intergenic_Dnah2|Efnb3_chr11_69553620_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Dnah2|Efnb3_chr11_69553620_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm4812|Papd7_chr13_69415080_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm4812|Papd7_chr13_69415080_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4930564D02Rik|1700095B22Rik_chr3_105180383_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_4930564D02Rik|1700095B22Rik_chr3_105180383_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm8644|Gm15171_chrX_157657618_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm8644|Gm15171_chrX_157657618_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm9621|Gm24200_chr9_94181709_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm9621|Gm24200_chr9_94181709_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Anapc10|Hhip_chr8_79789797_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Anapc10|Hhip_chr8_79789797_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Xylt1_chr7_117642511_F Primer3: not found at this Tm N.d. 0.02
mm4_intron_Xylt1_chr7_117642511_R Primer3: not found at this Tm N.d. 0.02
mm4_exon_Mrto4_chr4_139352453_F TCGTCGGCAGCGTCCGCCCAGTGTCCTGAAGG 60.0 0.02
mm4_exon_Mrto4_chr4_139352453_R GTCTCGTGGGCTCGGAGCCATAGAGAGGAACCGGT 60.0 0.02
mm4_intergenic_Caln1|Gm23761_chr5_130747305_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Caln1|Gm23761_chr5_130747305_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Krt35|Krt36_chr11_100097139_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Krt35|Krt36_chr11_100097139_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cbx3|Snx10_chr6_51512221_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Cbx3|Snx10_chr6_51512221_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Clec3b_chr9_123156301_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Clec3b_chr9_123156301_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmem173|Gm16490_chr18_35752293_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tmem173|Gm16490_chr18_35752293_R Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm9800|Fhit_chr14_9439771_F Primer3: not found at this Tm N.d. 0.01
mm3_intergenic_Gm9800|Fhit_chr14_9439771_R Primer3: not found at this Tm N.d. 0.01
mm3_intron_Hydin_chr8_110313247_F TCGTCGGCAGCGTCTCACATTCAGCCTTCAGAGCA 59.6 0.01
mm3_intron_Hydin_chr8_110313247_R GTCTCGTGGGCTCGGGCAGATCTCTGGCGTTAGCT 59.8 0.01
mm4_intergenic_Ninj1|1110007C09Rik_chr13_49199861_F TCGTCGGCAGCGTCGCTCACAGGTCTTTGAGGCA 60.2 0.01
mm4_intergenic_Ninj1|1110007C09Rik_chr13_49199861_R GTCTCGTGGGCTCGGTGTCAGTCAAAGGCTGGGAA 59.1 0.01
mm4_intron_Tspan18_chr2_93215974_F TCGTCGGCAGCGTCCAGGGTTCAGGGCACACTTA 59.6 0.01
mm4_intron_Tspan18_chr2_93215974_R GTCTCGTGGGCTCGGTAATGGGCCCATCTCTGCAG 59.5 0.01
mm4_intergenic_Rnf220|Gm12828_chr4_117427390_F TCGTCGGCAGCGTCTAAGCAAGAGAAGGGCAGCC 60.0 0.01
mm4_intergenic_Rnf220|Gm12828_chr4_117427390_R GTCTCGTGGGCTCGGTTCACGGACTGTTCGATCCC 59.7 0.01
mm4_intron_Gli2_chr1_118864994_F TCGTCGGCAGCGTCGCACCAGCCTAGAGTTTGGT 59.9 0.01
mm4_intron_Gli2_chr1_118864994_R GTCTCGTGGGCTCGGTACCTGGCAACCCTAGAGCT 59.9 0.01
mm4_intergenic_Obscn|Iba57_chr11_59138989_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Obscn|Iba57_chr11_59138989_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tshz3|Zfp536_chr7_37148399_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Tshz3|Zfp536_chr7_37148399_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Polk_chr13_96500953_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Polk_chr13_96500953_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Prodh|Rtn4r_chr16_18122733_F TCGTCGGCAGCGTCCTCCCTTGGGCTTAGGTTCC 59.7 0.01
mm4_intergenic_Prodh|Rtn4r_chr16_18122733_R GTCTCGTGGGCTCGGGCCATTCGATCCTCTGGGAG 59.9 0.01
mm4_intergenic_Gata6|Rbbp8_chr18_11352925_F TCGTCGGCAGCGTCTCTGTTTCAGCACAGGCCAA 60.1 0.01
mm4_intergenic_Gata6|Rbbp8_chr18_11352925_R GTCTCGTGGGCTCGGGGTGCCAGAGGGGAAAGAAG 60.3 0.01
mm4_intron_Cdc42bpg_chr19_6313094_F TCGTCGGCAGCGTCCCCATTGCTGTCCAAGGTCA 60.2 0.01
mm4_intron_Cdc42bpg_chr19_6313094_R GTCTCGTGGGCTCGGAAACACCCTGGCACATCGAT 59.9 0.01
mm4_intergenic_Gm24235|Gm25513_chr5_54828266_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm24235|Gm25513_chr5_54828266_R Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ablim1_chr19_57306097_F Primer3: not found at this Tm N.d. 0.01
mm4_intron_Ablim1_chr19_57306097_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22277|Lrrc30_chr17_67605807_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm22277|Lrrc30_chr17_67605807_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Prr18|Sft2d1/T2_chr17_8369910_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Prr18|Sft2d1/T2_chr17_8369910_R Primer3: not found at this Tm N.d. 0.01
mm2_intergenic_Gm5257|mmu-mir-6343_chr1_75853954_F Primer3: not found at this Tm N.d. 0.01
mm2_intergenic_Gm5257|mmu-mir-6343_chr1_75853954_R Primer3: not found at this Tm N.d. 0.01
mm2_intron_Creb5_chr6_53687839_F TCGTCGGCAGCGTCGCAGACACAGGTCAGACACA 59.8 0.00
mm2_intron_Creb5_chr6_53687839_R GTCTCGTGGGCTCGGGGGCATCTTTCTGTGTGGCT 60.6 0.00
mm4_intron_Usf2_chr7_30952267_F TCGTCGGCAGCGTCCTCTGCAACCCACCCTACAG 60.0 0.00
mm4_intron_Usf2_chr7_30952267_R GTCTCGTGGGCTCGGGCAGGGTGACTTTCAGAGCT 59.9 0.00
mm4_intron_Eif3f_chr7_108941367_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Eif3f_chr7_108941367_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ctf1/Ctf2_chr7_127717363_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Ctf1/Ctf2_chr7_127717363_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nrg4_chr9_55255777_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Nrg4_chr9_55255777_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Sumf1|Gm4604_chr6_108136626_F TCGTCGGCAGCGTCCAAAGCACCCACTGGCAAAC 60.5 0.00
mm4_intergenic_Sumf1|Gm4604_chr6_108136626_R GTCTCGTGGGCTCGGTCCTTCCATCTACTTAAAGGGCT 58.6 0.00
mm3_intergenic_Gm25875|Gm23835_chr15_60327500_F TCGTCGGCAGCGTCAGGGAGCAATTTAGCTGTTGT 57.8 0.00
mm3_intergenic_Gm25875|Gm23835_chr15_60327500_R GTCTCGTGGGCTCGGGCATTCCAGATCCCGGAGAG 59.9 0.00
mm4_intron_Gm5577_chr6_87982627_F TCGTCGGCAGCGTCACACCACTAAAGCGTCCAGG 59.9 0.00
mm4_intron_Gm5577_chr6_87982627_R GTCTCGTGGGCTCGGAAAGGCTGAGAATCTGGGCC 60.0 0.00
mm4_intergenic_Eya1|Msc_chr1_14646911_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Eya1|Msc_chr1_14646911_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Xpr1|Gm5532_chr1_155489478_F TCGTCGGCAGCGTCTTTCTGCTGCTACGGCGTAT 59.8 0.00
mm4_intergenic_Xpr1|Gm5532_chr1_155489478_R GTCTCGTGGGCTCGGTGCTACCAGACGCTTCCATG 60.1 0.00
mm4_intergenic_Gm12983|Gm12984_chr4_135295983_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12983|Gm12984_chr4_135295983_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pex14_chr4_149049087_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pex14_chr4_149049087_R Primer3: not found at this Tm N.d. 0.00
mm3_exon_C2cd5_chr6_143011925_F Primer3: not found at this Tm N.d. 0.00
mm3_exon_C2cd5_chr6_143011925_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22805|Gm4791_chr9_46736575_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22805|Gm4791_chr9_46736575_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pls1_chr9_95795529_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Pls1_chr9_95795529_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23848|Trps1_chr15_50501341_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23848|Trps1_chr15_50501341_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Soga2_chr17_66367798_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Soga2_chr17_66367798_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Slc4a4_chr5_89050915_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Slc4a4_chr5_89050915_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rims4_chr2_163882590_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Rims4_chr2_163882590_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mest_chr6_30732905_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mest_chr6_30732905_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mcc_chr18_44436036_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mcc_chr18_44436036_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Lrfn2_chr17_49033585_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Lrfn2_chr17_49033585_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Kidins220_chr12_25014563_F TCGTCGGCAGCGTCCACCTCCCTTTCCCTGGAAC 59.9 0.00
mm4_intron_Kidins220_chr12_25014563_R GTCTCGTGGGCTCGGCTGGGAGGACTGGAGGAAGA 59.9 0.00
mm4_intron_Fras1_chr5_96717529_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fras1_chr5_96717529_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fam214a_chr9_74990964_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Fam214a_chr9_74990964_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dnajc21_chr15_10468335_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Dnajc21_chr15_10468335_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Calb2_chr8_110154622_F TCGTCGGCAGCGTCTGTTACAACCTCAGCCGGAC 59.9 0.00
mm4_intron_Calb2_chr8_110154622_R GTCTCGTGGGCTCGGATTGAGATGGCGGAGGTGAG 59.5 0.00
mm4_intron_Cabp5_chr7_13403152_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Cabp5_chr7_13403152_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp8a2_chr14_59758974_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Atp8a2_chr14_59758974_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tnfrsf19|Sacs_chr14_61071971_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tnfrsf19|Sacs_chr14_61071971_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tln2|mmu-mir-7241_chr9_67611311_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tln2|mmu-mir-7241_chr9_67611311_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tbc1d22a|Gm23416_chr15_86746464_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Tbc1d22a|Gm23416_chr15_86746464_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pcdh18|Slc7a11_chr3_49903557_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Pcdh18|Slc7a11_chr3_49903557_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Npas3|Egln3_chr12_54166396_F TCGTCGGCAGCGTCATGGGATGGCCTTCTCCTCA 60.3 0.00
mm4_intergenic_Npas3|Egln3_chr12_54166396_R GTCTCGTGGGCTCGGCTATTGGAAGGTCCTGCCCG 60.1 0.00
mm4_intergenic_Notch1|Gm13358_chr2_26538817_F TCGTCGGCAGCGTCGCAGAGGAGTGGAGGGAAGA 60.6 0.00
mm4_intergenic_Notch1|Gm13358_chr2_26538817_R GTCTCGTGGGCTCGGTCAGGCAGGGGAATTTGCAT 59.9 0.00
mm4_intergenic_Mir3095|Gm12577_chr4_58468539_F TCGTCGGCAGCGTCAGAGGATGGCCCACTCCTTA 59.6 0.00
mm4_intergenic_Mir3095|Gm12577_chr4_58468539_R GTCTCGTGGGCTCGGCGGGAAGAGATAGAACACAATGC 59.4 0.00
mm4_intergenic_Hp|Dhodh_chr8_109587370_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Hp|Dhodh_chr8_109587370_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_H19/H19_1/Mir675/H19_2|Igf2_chr7_142594444_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_H19/H19_1/Mir675/H19_2|Igf2_chr7_142594444_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_95093683_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm9726/mmu-mir-8099-2|Gm26055_chr12_95093683_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm7488|Tbl1xr1_chr3_21360265_F TCGTCGGCAGCGTCTCATGGTAGTTGCCCAGCAA 59.5 0.00
mm4_intergenic_Gm7488|Tbl1xr1_chr3_21360265_R GTCTCGTGGGCTCGGCCCCATAGAAGAGACCAGGC 59.2 0.00
mm4_intergenic_Gm5909|Gypa_chr8_80368306_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm5909|Gypa_chr8_80368306_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26989|Vgf_chr5_137028279_F TCGTCGGCAGCGTCCCTCTCTGAACCACCAGCTT 59.3 0.00
mm4_intergenic_Gm26989|Vgf_chr5_137028279_R GTCTCGTGGGCTCGGACCCTCCTTCCCTTTTCCCT 60.1 0.00
mm4_intergenic_Gm23847|Fgfr2_chr7_130097414_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23847|Fgfr2_chr7_130097414_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23465|Vtcn1_chr3_100742299_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm23465|Vtcn1_chr3_100742299_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22777|Spock1_chr13_57164908_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22777|Spock1_chr13_57164908_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22494|Chrna7_chr7_62718083_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm22494|Chrna7_chr7_62718083_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm15011|Rbm41_chrX_139942004_F TCGTCGGCAGCGTCCAAGGAGGAGGTAAGGCTGC 60.1 0.00
mm4_intergenic_Gm15011|Rbm41_chrX_139942004_R GTCTCGTGGGCTCGGGAAGGAGCCACCCTGGTTAC 60.0 0.00
mm4_intergenic_Gm12210|Gm11187_chr11_52536080_F TCGTCGGCAGCGTCTGGAGTGGGGAAAATGGCTG 60.2 0.00
mm4_intergenic_Gm12210|Gm11187_chr11_52536080_R GTCTCGTGGGCTCGGTCATTGCTTTCCCTCCCAGC 60.3 0.00
mm4_intergenic_Barx1|Phf2_chr13_48756890_F TCGTCGGCAGCGTCGCTTGAGGGTTGGCTGTGTA 60.2 0.00
mm4_intergenic_Barx1|Phf2_chr13_48756890_R GTCTCGTGGGCTCGGCGGTGTGTTTAACGTCAGCG 60.1 0.00
mm4_exon_Fgd3_chr13_49296769_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Fgd3_chr13_49296769_R Primer3: not found at this Tm N.d. 0.00
mm4_exon_Brsk2_chr7_142002978_F TCGTCGGCAGCGTCTAAAAGACCTGGGTGCTCCA 58.5 0.00
mm4_exon_Brsk2_chr7_142002978_R GTCTCGTGGGCTCGGGCTGCTGCTACTCCTCCTTC 60.1 0.00
mm4_exon_Acvr1_chr2_58447380_F Primer3: not found at this Tm N.d. 0.00
mm4_exon_Acvr1_chr2_58447380_R Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Lrrc7|Gm9423_chr3_158714548_F TCGTCGGCAGCGTCACCAAGATAATCCTGTGTTCCA 57.0 0.00
mm3_intergenic_Lrrc7|Gm9423_chr3_158714548_R GTCTCGTGGGCTCGGGCCAATTACTAGTGACCATGGC 59.3 0.00
mm3_intergenic_Cdh9|Gm22032_chr15_17131972_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Cdh9|Gm22032_chr15_17131972_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313759 CTGCCTTGTCGCCTTAGCTCCTCTTCTCAGCCAAGATCCCAGGGAGCCTGGGATTAGGAGCTTACTTGGGGGTTTTCCCC
CTCCCCCCCTCTGGAGAGTCCGGGGATGGAGAGCCGAAAGGAC
mm3_intergenic_Nup93|Mir138-2_chr8_94315245 GTGCTGCTGTTCTCTGGAGTAGTCTTTGAGGTCATCTAGCCTCACACCAGCCCTCATTCCTCAGCCAAGATCCCAGGAGA
TTAATCTGCTGGTGTACGTTCCAGAGACGG
mm4_intergenic_n-R5s193|Spsb1_chr4_149856420 CGGTGACTTGCAGCTGACTATGGCTGGGAGTTGGAGGGTGGCCTAGAGCCGGGGCTGGATCCTGCCTGGGATCTTGTCTG
AGGAGCC
GCCATGTGCTGTCAGGTCTCAGAGCCAGTGTCT
mm3_intergenic_Gm25818|Creb5_chr6_53435031 AATGCAGTTCCGAACCACCTCCTTGCCTCCTGCCCAGGAATTTTGGCTGAAAAGAGACTTGTGCTCAGTCTGGTGATGGC
TT
mm3_intergenic_Sema3e|Pclo_chr5_14386651 TGTTACGGATTTCCAACCGCGTCTTCTTAGCCAAGATTCCCGGGCTGATGCTTGCACAACCTGCAGGATTACCTGCGAGG
AGCAGGAGATGGAGGGATGCCGGGGTTCTTCCCCGACCCCAGGGTCATAGCTGCTAGC
mm3_intergenic_Gm5046|Gm23907_chr15_65313530 CTTTGCATGTGTGGAAGGGCACACCTGGGATCATGGATGAGAAGGGGAAGATATGGAAACAAACAAATTTGCATGAAAAT
GCACAACGAATCAAAGTACTGT
mm4_intron_Piezo2_chr18_63217413 CTGAGGGAAAGAGCCAGTGGCAGAAACTATCTTACTATCTTTTGCCTTTTCAGCCAAGATCTCTGGTCATCAGTAGATAT
TGCTTATGTCCTTGGCTATTTCTTCATCTGGGAGGGGGCAGTTTGCTGGAAATGCA
mm3_intron_Agk_chr6_40347787 ACCCTGAATCTGGAATAGCCCCCAGGGATCTTGGTTCAGAACAGATTTTACCTCTGAAAGTCAAGTCTTGTTAAAATTGT
TTTCTTCTGCTTTTAAAGCCCATGTCACTAACATAGCTGATTCCTAGCACTGCACTGCC
mm4_exon_Lrrc4_chr6_28831642 TGCACAGTTACCTGCCACAAGAGCTTCATGGTGTGGCACGTTCATAATTCACCATCGCCTGGGATTTTGGCTTGGAAAAG
AGAACCAGCCTTACCCCGGCTTAAGTGAGCTAG
mm4_intron_Nrxn2_chr19_6511468 GCTAGGAGGAAGGCATGGTGTCTGCTATCCTCAGCCAGGACCCCTGGGTAGGTAGAGAGAAAAGCCGTCTGGCATCCCTT
GCCTGTAAATCTGGGTATTTTTAAATTTTGGGGTGTTGGAGGTAGG
mm4_intergenic_Cpeb2|C1qtnf7_chr5_43435525 CCCATATTTCATGACAGCCAACCTGTACAGGACACTCATTTCATACAAGATCCCAGGCTCTTGCTTCCAGACACTGTGCT
mm4_intron_Rps6ka1_chr4_133864534 TGGACTGGACCTTAGCTGGTTGTGATCCAGATAAAGCCAGCATCCAAAAACCTCGGCAAATACTGTATACTTCACTGAAC
AACAGGAATGTCCTTCTCTCATCAAAGATCCCCGGGAGCCAGGGCAGAATCAGTGTCT
mm4_intergenic_Itm2b|Med4_chr14_73488926 ATGAGGTGGCCAGCTGAAAAGCTCATGCAGAAGGCTTGATTAGCTCAAGTAGTGATGTAACACTTCCCCTCCTTCCAAGG
GCCAGGAGCAGATCTTGTCAACTAAGATCCCTGGAGTTGCAGAGTCCTGAGCTTGAGG
mm4_exon_Rnf165_chr18_77462977 TCCCCTTCATCTTTCTTGCCCTTGCTGTCCTGGGGTCTTCGCTGTGAATAGAGAAAGAAAAAGGTTTTAGTTCCTTGGTA
GAGACTCCTGCTGGCAACTG
mm3_intron_Arhgap15_chr2_44287005 TCAAGCAACTCTCCAGTGGTAGAAAACACCACAGCCCAGAGATCTTGGCTGAGAACATTTTAGTCCTTTTCTTTGAACTT
CACTCTGGGGGCAGGTAACTGAGAGTAC
mm4_intron_Tnni1_chr1_135798567 CTGAGAGCCTGCTTCTGACCTCCCAGAGGGGGTCAGTCCTCTGCCTGTCCTGCCTAACCTGGGATCTTGGCCTACAAGAT
GACTGACTATAATAGCCACTGGATTAACATAGCAGGCATTGTCTTTCTCTGA
mm4_exon_Flnc_chr6_29454413 CACCCGGAGACTACAGCATCATCGTGCGCTTTGACGACAAGCACATCCCAGGGAGCCCCTTCACGGCCAAGATCACAGGT
GAGGCAGACATTCAGGCACGGGCAGCCTGCCACACCCATGCTCGAGTCAGTT
mm4_exon_Flnb_chr14_7927497 GCCAGGGGACTACAGCATTCTGGTCAAGTACAATGACAAACATATTCCTGGCAGCCCCTTCACTGCCAAGATCACAGGTA
GGGTTGCCCGGCTTATAGGAATTTCTCATGGGAGATCTAGCTGTGCCT
mm4_intergenic_Gm13668|A630050E04Rik_chr2_74084756 GAGACTGTGGCCAAGGACTCCAAAGAACTCTGAAGCTTTAATTTAACCCAACTAAAACTCTAAAGAGAAAAGAGAAGACT
ATTAATGAAAGCTGTTCTCAACCAAGACACCGGGTCAGAGCACTTATTGGCAGAAGA
mm4_exon_Adamts14_chr10_61198415 GGAACCTGTGCTACCTCCTGTCCCCTGCGCTTGAGTCCTCTGAGCCAGGATCCCTGGGACCACAGGCAGAGTGTAAAAAA
GACTGGAGGGATGGGCTCATGTCAC
mm4_intergenic_Gm13994|Slc24a5_chr2_125046183 CAGTGGGCATTGCTGGAAAAGTTCAATAGTTTGATATCCAGTCAGGCCAAAGTACTGCAATATCAAATCATCTGAGCCAA
GATACCAGG
TAACATCAAGGAAGAAAGACTTCCCACCACTTAGAGGGGA
mm4_intergenic_Rdh10|Stau2_chr1_16173476 CCAGGAAACCACCCTCTGTGGGGTGATTAGACTGGTCTCAGCAAAGACCCCAGGCTTGAACGATGTCCCTCAGGACTCCA
CAACAC
mm4_intron_Klhl29_chr12_5273493 TCCAAGTGATCACAGCTGCCAAGAAAGAAATCAACTCAGCCAAGATTCCAGGCCTGACGGGATCCAGGGTTTCCTA
mm4_intergenic_Ccnd1|Tpcn2_chr7_145020987 CTAATGCCCTCCTGGACCTGGACAGCCAACCAGAGATCATTACTGATGCTCATGTTGGCAGAACCCAGGTTTTTGGCTGA
GGAGAG
AGCTTGGGATTCTTGCTGATACCTTGTCCTAGTGGAACAGATGCGCATGCTGAG
mm4_intergenic_Gm25261|Gm831_chr4_131359776 TGAGGGAGGAACAGGTTGGAGGGACCCGTTGGGTCTCAGTCACTTCTCTCTGATGTTCTCCTCTCAGCCAGGACTCCAGG
AGGCCAAGGAGGAGGGGACATGGCTGGTCCTTTCTGTCTCC
mm4_intron_Rgs3_chr4_62619998 CGGGTTATAGGCAGCTGAGGCCAGTTCTGAGTGTTTTCCTATCCACTGCCTGGGATCCTTGATGAGATGAGTTAGCTGTT
GCTGCTTGGTTTT
mm4_intergenic_Spata13|C1qtnf9_chr14_60766680 GGTAACTTGCCGCATCCTCTGCACCAAAAGCTGAAGTCCGGGGTTCCTGGAAGAGAAGAGTAATTGTCAGGTCCGAAAGG
AACCTCGGTT
mm4_intergenic_Gm22785|Gm8410_chrX_70693980 TGACCACCAGGAGAAGACCATGCCCAAAATGACATTTGCCTGATGCCATAATGCACATGTGGGTCATAGCAATCCTGAGA
CCACTCCTGGGATCTCTCCTGAGAAGATCCCAGACACTCAGAGACCC
mm4_intergenic_Gm15867|Syt14_chr1_192891006 GGTTAGATCTGACTCGTGGTGAAGAAAACATGATCCAGATAGTTTGACAGCACATGTGACTCTTTTCAGACATGATCCTG
GG
AACAAACAGACCCACTTGATTATTTCTGTATGTAAGTGACATTGGCCCATTGTGT
mm4_intron_Rbfox3_chr11_118754506 ACAGGCCATGTGTGTGTCAACCAAGGGTCTTGGCTGAGAACAAGAAGGAAGTCAGCACAGAGAATGGGGCTCACTCTATG
GTGTG
mm4_intron_Mtch1_chr17_29339978 CCTCTGACTGGGAAGGACCTGAGCTCGTCTCCTGTCGCCCCCTGCCTCTTCCCAGCCATAATCCTCGGCTTATGGTGGGG
CCAGCCTACACCCACCCATCTGTACTTTAGTGGGCTCAGCTGCCATGCTCTTACC
mm4_intergenic_Gm23673|Gm9381_chr3_124934462 CTCACTCCTACAGGGAAAACCATCTTCTTAATTTAAGATTAAAAGTAGATATATTTATATCCGCAATCAACTTTTTTGAC
AATAGAGACCCGGGTTCTTGACTGTGAAAAGTAAATTGGCCCTGGCTGTTGA
mm4_intergenic_Plb1|Gm5864_chr5_32398748 CCAGTGCCAATTTGTCCCCTACCTTCAGGTGCTATATCCCCCTGTCAGATTTTTGTTTAAACTTCTCAGCCCAGAACCCA
GG
GTCCTTGGTCATCTGGCTCC
mm4_intron_Tmem132d_chr5_128103070 TCTACTCAGCTGGGGTCACTGCCAGACCCTCCTGGGTACTTTAGTCAGCTAAGATCCCTGGGAGGCGGGTGAGATAATTC
TGC
mm4_intergenic_Gm3104|Gm7353_chr7_3092054 GCCTCACTTGAGTGGAGGAAAACAGGAAAGCTCTAGGGGAGCAGATAAGTTATTTTTCAGGGCCTGAGGATCTAAAACCC
CAGGTGTCTTGGGAGAGAAGAG
AATACTTTGGTGGTATCCCAGCTGGAAAGAGATGGT
mm3_intergenic_Atp1b3|BC043934_chr9_96398824 CAGAGAGGAAGGGAGGCCTCCGGGAAGGCTTTGACCCCCTCCTTCTCTACTTAGCCAAGGTCCCTGGCTTGTGCAGCAGG
GTTTTAATATTTCCAGCGGAACCACAGACAAGGTGGTAAGGAATGGGGAAGGGTGTGTT
mm4_intron_Odf2l_chr3_145146101 CTGCTGCTTGAGTCTCCTCATTTTTCTCCTAGGATCTTGTCTGGGTAGAGGTTCAGAGGTTGCTTGCTTATCTTTTGTCT
TCATGGCCAGAGGGTGCATGTGCTGTGAGTGCTCAGCTCAGACACCTT
mm4_intergenic_Gm24132|Gm25160_chr8_96728242 AGGCCTACTAGATGGTTTCCATCTCCTGGAATATTTGCTGAGGAGAGCTGCAAGGTATCCTTTTAGCAGAAGTCCGCTTG
TTCTCTATATGTGATGGGGCCAGGGACACTAAAAT
mm4_intergenic_Camk2n1|Vwa5b1_chr4_138516640 GGTGAGATCATTGGTGGCCACATTCCATAGACCTCAGAATTAATGTGTTACTAGTCCCCCAGGGACCTTGGCAGAGAGTA
G
GGCTGGCTCCTTGTCAATACAGTTCCCCCATGCAGAGGTGTGGCCCAGTATG
mm4_exon_Reps2_chrX_162416457 ACAGCCAAGGATTGTTGCAGAGTTAGTGGCTATTTTTCTTCCCTTCTCAGCCTGGATACCCGGATTCAACAACAAATAGG
TAGCTATCCAGCTACTAATATTTGTTGGGTGGTTTCCAAGCT
mm4_intron_Postn_chr3_54364662 TGTGCTTTCTGTGAACAAAACCTAAACGTTTTTGTTGTATTTGAAACATCCCCTCAGATAAACACATTGTCCACTTCTCA
GCTGAGGTCCCAGG
CCACTGTTCTGCAAGCTCGCCCCTTCCCCAGCATGACTTT
mm4_intergenic_Sall1|Gm6625_chr8_89076014 GGGCGGCCATAGCATATCTATCACTGTCACCCCTGTTCCTGGGTTCTAGGCTGAGCACAGGTGTCCTCCCTGGTCCCCAA
TTCAGGAATTTCCAACCCACATGGACATTTACCTTGAAGCAGAGAGGCAA
mm4_intron_Cntnap5b_chr1_100038514 TGTGTGAACAAGTTTACCCAGTGTTAATCCTCTAGATTATATCTGCTGCCTCCTGATCCCTGTGATGTTTGCAGAGAAGA
G
CAGTAGAGAGCATAATTAATGCCTGTGGAGGAAGCAT
mm4_intergenic_Gm26253|Rab1_chr11_20200928 TTAGAGCAGCCTCAGACCCTGATGGTGATGGCCCCTGTGGTCTTGCCTGAGCAGAGCTCGTCAAGCTGTCTCCACCCCTC
CTCA
mm4_intergenic_4933413G19Rik|Nrip2_chr6_128393461 CAGGGCAGTGTCCTCTCTTGGTAATAGATTTCAAAATTGGCACTGGGTTCCAGGGACCCTTGCTGAGCAGAGGAATTCTC
GAAAGCCACATCATTCATGCCTTCAGCGGA
mm4_intron_Prkd1_chr12_50639115 TTTCCCACCTGTCCAAGTGGCCAACTTTACAGTCTCCTGTCTCAGCTTAAATTGTTCAGAGTAAGATGACCTGGGATCTA
GACCTAGAAGAG
ATTTAAGGATAACTGTGTGTGGAATCCAAAAGCCTGAGGT
mm4_intron_Abca4_chr3_122139964 GTCCTGGGTCAATCCTGCTCTTTATATTGCTCAGGCAATAGATTGTCCCTTTGGGTGACAGCTACATTGCCACATTGCAG
ATATCCCTGACTCCCTAGGATCTGGGCTGAGAAAGGAAACCATCAGACCTCCATGCACA
mm4_exon_Ndrg3_chr2_156935364 AATGGACGGATGGACAGACGGCTGCCACGCCCCAGCCTCTGACTCTTCTGACTCAAGATCTCTGGCTCTCCAGTGTGAAG
CTGAATTCTTTGTGACTGTTCCATTGTGATGGTCATCAAAATCGGGGATGATGGCCTG
mm4_intergenic_Gm12126|Tenm2_chr11_36096384 CTGGCCTCTCTCCAATTCCCCCAGAGCTCTTGGCCGAGAAGATGGAAAACCAAAGAAAAGAGCACAGGCCTTGGGCAGCT
GCTCTTGCGCGCTGCAGAGAACGCCTCGCTATT
mm4_intergenic_Gm14055|Gm14064_chr2_137375075 GGCTTTAGAACCCAGCTCCACTTACCATCTGTCTGTTAGCACCACGAATCTTGGCTGAGAAGGATGCTTTGCAGATCTGG
GAATTCCATTCCCCCCCTTTTTCCCCCAAGCACCTAGGGATGGGGCTTTCCAGA
mm4_intron_Prmt3_chr7_49824514 CAACAGCCTTGAAGTCGCACCTCTTCTCTGGCAGGATCTCTGGGTCTGACCCACTGCCTGTGTTGGGGACAAGGCTTT
mm4_intron_Alg5_chr3_54748240 GCCTTCCAGGATAGGCTTCCATAGCTGCCAGGGCTACCCTGTTCCAGACAGGACAGGGAGGGGACAGGGACCTTTAATCA
GAAAAACACCCAGGGTCCCTGGCTGAGAGGAGAGAGTGGAGAAGCAGCTGTGAGTG
mm4_intergenic_Cadm1|Nxpe2_chr9_47905177 AATCCGTCCTGCCTTGAGTGTGCAGGCTCCACCGCTCTCTCTGTCAGTAAGTTCTGCATCCCTCTCTGCACAGGCCAGAT
CCCTGG
CATTCCTGTCCACCAGGAGCAAGACCTTGT
mm4_intergenic_2310015A10Rik|Actn1_chr12_80137741 CCCTTCCTCACGTCTTGTCCAAAGGCTTGGAGCCTGAGAAGGAGACTGGGGAAGGAGTTCTGTTCCCAGCCAATAACCCT
GG
GCAGCTCAGTCAGAGCCACCAGCAGAGGGACGTTCAAT
mm4_exon_Mrto4_chr4_139352453 CGCCCAGTGTCCTGAAGGCCACTTCTCTGCCCAGGTCCCGGGTCCCTCCAGTCCCGGCGGCTTCCGAGTCCCGAGCACTC
CACCAAGTCCCTCGCGAACCGGTTCCTCTCTATGGCT
mm3_intron_Hydin_chr8_110313247 TCACATTCAGCCTTCAGAGCAAAAACATCGCCAGGGCTCAAGGCTGAGAAGAGGTTCTCCATAGAGGACTGAAGTACCTT
AGAGTCCCCCTCAAACACAGCAGGGAAGGAAGAGCTAACGCCAGAGATCTGC
mm4_intergenic_Ninj1|1110007C09Rik_chr13_49199861 GCTCACAGGTCTTTGAGGCATCTCTAAAGAAGGGGTATATGCAATGGTGTCAAATGGGGACCAGACCCAGGATCTTCTCA
GCCACCATCCTAGG
CATTCCCAGCCTTTGACTGACA
mm4_intron_Tspan18_chr2_93215974 CAGGGTTCAGGGCACACTTAGCCGAGGCTGGCAAAGCAGCCAAGCACAGCCTGCTCTCAGCCAAGAGCACAGGCTGCCTT
CCGGGCTTGGCTGCAGAGATGGGCCCATTA
mm4_intergenic_Rnf220|Gm12828_chr4_117427390 TAAGCAAGAGAAGGGCAGCCCCTGTTCGTGAGAAGAAAGAGCGAGAGTCTGGGAGGCTGCTCTTCTCCCCCAAGAAGCCA
GG
CAGTTGCTGAATAACAGGGATCGAACAGTCCGTGAA
mm4_intron_Gli2_chr1_118864994 GCACCAGCCTAGAGTTTGGTGGTGGTTCCCCACGCCTCTACTCAGCCCACATACCAGGCATCCTTGGACAGCTCTAGGGT
TGCCAGGTA
mm4_intergenic_Prodh|Rtn4r_chr16_18122733 CTCCCTTGGGCTTAGGTTCCCCATATGTACCTTGATGAGGATCCCAGGGATGAGGGGTGAGAAGAGCCAAGATCCAGCAA
AAGGATGAACTGACCTCCCAGAGGATCGAATGGC
mm4_intergenic_Gata6|Rbbp8_chr18_11352925 TCTGTTTCAGCACAGGCCAAAGCCCTCTTCCCTGACACCAGCGATCTTCCCTAAGAAGAGATCTGGCCCCGTTTGGAGGC
AGATGTTGGATTTTCTTCTTTCCCCTCTGGCACC
mm4_intron_Cdc42bpg_chr19_6313094 CCCATTGCTGTCCAAGGTCACTTGTATAGCCCCAAAGGCAGGGTGTGGTCTAGTGCTTCTCTTCTCAGCATGGAGCCCAG
G
ACCTGGGTCTGGATCGATGTGCCAGGGTGTTT
mm2_intron_Creb5_chr6_53687839 GCAGACACAGGTCAGACACAAAACGTTAAATCAGGGTGAATTAAAACGAGTCCCTACAGATTTACTCCAGGATCTTCGCT
GAGAAGAG
TCATAGCAGAGCCACACAGAAAGATGCCC
mm4_intron_Usf2_chr7_30952267 CTCTGCAACCCACCCTACAGAAAAACCACAGGGGAAAAAGACGGAGAAGCTACATGGAGACTCTTGTCAGCACAGAGCCC
TGG
GAAGGAAAGGGTAGAGCTCTGAAAGTCACCCTGC
mm4_intergenic_Sumf1|Gm4604_chr6_108136626 CAAAGCACCCACTGGCAAACCCTGCTCTTCTCAGTCAACATTGCTGGCCCAATCAGGTGTCATACACTATAAAAGACAGA
GCTCATAAAAGCCCTTTAAGTAGATGGAAGGA
mm3_intergenic_Gm25875|Gm23835_chr15_60327500 AGGGAGCAATTTAGCTGTTGTTGGAGGATAAGTCAGGGATCTGGGCTGAGATGTGGGTGAAAATGGGCACAGCCATTAGC
ATTATTTTCTCTCCGGGATCTGGAATGC
mm4_intron_Gm5577_chr6_87982627 ACACCACTAAAGCGTCCAGGCCAGCTTTGTTCCAATAGGTAAGCAGTTCCCTGGCCTCTTGGGTGAAAAGAGGGAGTGAC
TGCCTGTAGATCAAGAAGGCCCAGATTCTCAGCCTTT
mm4_intergenic_Xpr1|Gm5532_chr1_155489478 TTTCTGCTGCTACGGCGTATGCCAGGCATCTGGGGTGAGATGAGAAAAATGGGAACTCGAAAGCCTTTGAAACTGCAATG
ATCATGGAAGCGTCTGGTAGCA
mm4_intron_Kidins220_chr12_25014563 CACCTCCCTTTCCCTGGAACTTCTCCTGGCTCTGCCCCTCCTGGCTCTGCCCCTGCCTTGGCCCTGCTTCTGTTCTCAGC
AAAGCTCCTTGG
TAGAGCACACTGCCCTCTCCCATGCTGTTCTTCCTCCAGTCCTCCCAG
mm4_intron_Calb2_chr8_110154622 TGTTACAACCTCAGCCGGACCCCTGGCACTCCTCAGCCACGCCCCCTGGCTCTCCTCAGCCACGCCCCCTGGCTCTCACC
TCCGCCATCTCAAT
mm4_intergenic_Npas3|Egln3_chr12_54166396 ATGGGATGGCCTTCTCCTCAGATCTCAGGAAGCTTCCTGGGAACTGTGCTGAGAAGCGCCAATGGATGCTCGGGCAGGAC
CTTCCAATAG
mm4_intergenic_Notch1|Gm13358_chr2_26538817 GCAGAGGAGTGGAGGGAAGAAAAAAGAGCAGCTATCTGGGCTGGCCCCTCCTCTCAGCGGAGCTCCCAGGCTTTCCCCTT
CCACAGGATGCAAATTCCCCTGCCTGA
mm4_intergenic_Mir3095|Gm12577_chr4_58468539 AGAGGATGGCCCACTCCTTATATAGCCTGGGAAGAAGTAAACAGCCAGTGTCCCCTTCTCAACAAAGTTCCCAGGCAGAG
ATATATAACTGTTACTGGCTTAATTTAAAAAGGGGGGCATTGTGTTCTATCTCTTCCCG
mm4_intergenic_Gm7488|Tbl1xr1_chr3_21360265 TCATGGTAGTTGCCCAGCAAGAGTTTGTAGCTGAACTATCAAGCCATCCCCTGTCCCACCAGTCTCCTCTCAGCCCAGTT
CTCAGG
TTTAGCCTGGTCTCTTCTATGGGG
mm4_intergenic_Gm26989|Vgf_chr5_137028279 CCTCTCTGAACCACCAGCTTTCTGATTCACCTTCCATAAGGAGGATAACGTGACCCCCCCCCCCCCAGCCCACGGCTCCA
GGGAACCAGGCTGAGAAGAT
GAAGAAAGATGGGGAGGGAAAAGGGAAGGAGGGT
mm4_intergenic_Gm15011|Rbm41_chrX_139942004 CAAGGAGGAGGTAAGGCTGCCCACCTGAGAGGAAGCAGTCCCTTCCGGGAAACCTGGCTGAGAAGTGTAGCCCAGGGTAA
CATGTGCAAGAGGCTGTAACCAGGGTGGCTCCTTC
mm4_intergenic_Gm12210|Gm11187_chr11_52536080 TGGAGTGGGGAAAATGGCTGTCATATCTCCCAGAGAGCTTGGCTGAGAGGTGCTGCTTGCTGGGAGGGAAAGCAATGA
mm4_intergenic_Barx1|Phf2_chr13_48756890 GCTTGAGGGTTGGCTGTGTAAATATTTGGCCTTCGGGGCTCTTCATAGCCAAGCTCCTCGGGGCGCTGACGTTAAACACA
CCG
mm4_exon_Brsk2_chr7_142002978 TAAAAGACCTGGGTGCTCCAGAGCTGCCAGGGAGCTGGGCTGAGACCAGTATTATTTATTTGCTGTTTTAATTTATTGAG
TTTCTTTATGTCTCTGTTCTCTGTTCAGGGAAGGAGGAGTAGCAGCAGC
mm3_intergenic_Lrrc7|Gm9423_chr3_158714548 ACCAAGATAATCCTGTGTTCCATCTAATCTCCAGGGGACTTGGGTGAGAAGAGGGAGGACAAACTAATAAATCTGTTGCA
TCTGTAATACTGCTAATTTTATGTTAGACTAACAAGCCATGGTCACTAGTAATTGGC

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

Version 5.01 - Documentation  - Contact us - Downloads/local installation - Citation - License
CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.