CRISPOR

DNA break repair outcome predictions

Guide: CTCCTAATCCCAGGCTCCCT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGGAAAACCCCCAAGTAAGCTCCTAATCCCAGGCTCCCTGGGATCTTGGCTGAGAAGAGGAGCTAAGGCGACAAGGCAG`	(Wild-type sequence)
314	`CCCAAGTAAGCTCCTAATCCCAGGCT----------------GAGAAGAGGAGCTAAGGC`	`16 bp deleted → frameshift`
268	`CCCAAGTAAGCTCCTAATCCCAGG--------GATCTTGGCTGAGAAGAGGAGCTAAGGC`	`8 bp deleted → frameshift`
255	`CCCAAGTAAGCTCCTAATCCCAGG---------ATCTTGGCTGAGAAGAGGAGCTAAGGC`	`9 bp deleted → no frameshift`
211	`CCCAAGTAAGCTCCTAATCCCAGGCTCCCT-------TGGCTGAGAAGAGGAGCTAAGGC`	`7 bp deleted → frameshift`
191	`CCCAAGTAAGCTCCTAATCCCAGGCTC---------TTGGCTGAGAAGAGGAGCTAAGGC`	`9 bp deleted → no frameshift`
173	`CCCAAGTAAGCTCCTAATCCCAGGCT-----------TGGCTGAGAAGAGGAGCTAAGGC`	`11 bp deleted → frameshift`
170	`CCCAAGTAAGCTCCTAATC-----------------TTGGCTGAGAAGAGGAGCTAAGGC`	`17 bp deleted → frameshift`
164	`CCCAAGTAAGCTCCTAATCCCAGGCTCCCT------------GAGAAGAGGAGCTAAGGC`	`12 bp deleted → no frameshift`
129	`CCCAAGTAAGCTCCTAATCCCAGG---------------------------AGCTAAGGC`	`27 bp deleted → no frameshift`
121	`CCCAAGTAAGCTCCTAATCCCAGGC-----------------------------------`	`35 bp deleted → frameshift`
117	`CCCAAGTAAGCTCCTAATCCCAGGCT-----------------------------AAGGC`	`29 bp deleted → frameshift`
111	`CCCAAGTAAGCT------------------------------GAGAAGAGGAGCTAAGGC`	`30 bp deleted → no frameshift`
99	`CCCAAGTAAGCTCCTAATCCCAG----------------------AAGAGGAGCTAAGGC`	`22 bp deleted → frameshift`
99	`CCCAAGTAAGCTCCT----------------------TGGCTGAGAAGAGGAGCTAAGGC`	`22 bp deleted → frameshift`
95	`CCCAAGTAAGCTC-----------------------TTGGCTGAGAAGAGGAGCTAAGGC`	`23 bp deleted → frameshift`
86	`CCCAAGTAAGCTCCTAATCCCAGGCTCCCT-------------------------AAGGC`	`25 bp deleted → frameshift`
86	`CCCAAGTAAGCTCCTAATCCCAG-------------------------AGGAGCTAAGGC`	`25 bp deleted → frameshift`
86	`CCCAAGTAAGCT-------------------------TGGCTGAGAAGAGGAGCTAAGGC`	`25 bp deleted → frameshift`
77	`CCCAAGTAAGCTCCT---------------------------GAGAAGAGGAGCTAAGGC`	`27 bp deleted → no frameshift`
69	`CCCAAGTAAGCT-------------------------------------------AAGGC`	`43 bp deleted → frameshift`
67	`CCCAAGTAAGCTCCTAA----------------------------------------GGC`	`40 bp deleted → frameshift`
66	`CCCAAGTAAGCTCCTAATCCCAG------------------------------CTAAGGC`	`30 bp deleted → no frameshift`
60	`CCCAAGTAAG--------------------------------------AGGAGCTAAGGC`	`38 bp deleted → frameshift`
52	`CCCAAGTAAG-----------------------------------AAGAGGAGCTAAGGC`	`35 bp deleted → frameshift`
48	`CCCAAG------------------------------------------AGGAGCTAAGGC`	`42 bp deleted → no frameshift`
45	`CCCAAGTAAG------------------------------------------------GC`	`48 bp deleted → no frameshift`
44	`CCCAAGTAAGCTCCTAA------------------------------GAGGAGCTAAGGC`	`30 bp deleted → no frameshift`
42	`CCCAAG---------------------------------------AAGAGGAGCTAAGGC`	`39 bp deleted → no frameshift`
40	`CCCAAGTAAG----------------------------------------GAGCTAAGGC`	`40 bp deleted → frameshift`
34	`CCCAAGTAAGC-------------------------------------------------`	`49 bp deleted → frameshift`
33	`CCCAAG--------------------------------------------GAGCTAAGGC`	`44 bp deleted → frameshift`
29	`CCCAAG----------------------------------------------------GC`	`52 bp deleted → frameshift`
28	`CCCAAG-----------------------------------------------CTAAGGC`	`47 bp deleted → frameshift`

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