CRISPOR

DNA break repair outcome predictions

Guide: GCTTAGAAAGAAGAGGGCAA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGC`	(Wild-type sequence)
536	`GAGGATGACAGCTTAGAAAGAAGAGGGC--------TTCCTCCCAGAGGCGGTGCGGCAC`	`8 bp deleted → frameshift`
423	`GAGGATGACAGCTTAGAAAGAAGAGGG-------GCTTCCTCCCAGAGGCGGTGCGGCAC`	`7 bp deleted → frameshift`
296	`GAGGATGACAGCTTAGAAAGAAGAGGG------GGCTTCCTCCCAGAGGCGGTGCGGCAC`	`6 bp deleted → no frameshift`
255	`GAGGATGACAGCTTAGAAAGAAGAGG---------CTTCCTCCCAGAGGCGGTGCGGCAC`	`9 bp deleted → no frameshift`
253	`GAGGATGACAGCTTAGAAAGAAGAGG-----------------------CGGTGCGGCAC`	`23 bp deleted → frameshift`
199	`GAGGATGACAGCTTAGAAAGAAGAGGGC----------------------GGTGCGGCAC`	`22 bp deleted → frameshift`
180	`GAGGATGACAGCTT------------------------CCTCCCAGAGGCGGTGCGGCAC`	`24 bp deleted → no frameshift`
156	`GAGGATGACAGCTTAGAAAGAAGAGGGCA------------------------------C`	`30 bp deleted → no frameshift`
134	`GAGGATGACAGCTTAGAAAGAAGAGGGCA----------------GAGGCGGTGCGGCAC`	`16 bp deleted → frameshift`
114	`GAGGATGACAGCTTAGAAAGAAGAGGG-------------------------TGCGGCAC`	`25 bp deleted → frameshift`
109	`GAGGATGACAGCTTAGAAAGAAGAGG--------------------------TGCGGCAC`	`26 bp deleted → frameshift`
109	`GAGGATGACAGCTTAGAAAGA--------------------------GGCGGTGCGGCAC`	`26 bp deleted → frameshift`
105	`GAGGATGACAGCTTAGAAAGAAGAG---------------------AGGCGGTGCGGCAC`	`21 bp deleted → no frameshift`
103	`GAGGATGACAGCTTAGAAAGAAGAGGGC---------------------------GGCAC`	`27 bp deleted → no frameshift`
94	`GAGG-----------------------------GGCTTCCTCCCAGAGGCGGTGCGGCAC`	`29 bp deleted → frameshift`
89	`GAGGATGACAGCTTAGA------------------------------GGCGGTGCGGCAC`	`30 bp deleted → no frameshift`
89	`GAGG------------------------------GCTTCCTCCCAGAGGCGGTGCGGCAC`	`30 bp deleted → no frameshift`
87	`GAGGATGACAG-----------------------------------AGGCGGTGCGGCAC`	`35 bp deleted → frameshift`
86	`GAGGATGACAGCTTAGAAAGAAG-------------------------GCGGTGCGGCAC`	`25 bp deleted → frameshift`
86	`GAGGATG-------------------------GGGCTTCCTCCCAGAGGCGGTGCGGCAC`	`25 bp deleted → frameshift`
84	`GAGGATGACAGCTTAGAAAGAAGAGG-------------------------------CAC`	`31 bp deleted → frameshift`
84	`GAGG-------------------------------CTTCCTCCCAGAGGCGGTGCGGCAC`	`31 bp deleted → frameshift`
74	`GAGGATGACAGCTTAGAAAG----------------------------GCGGTGCGGCAC`	`28 bp deleted → frameshift`
74	`GAGGATGACAGCT----------------------------CCCAGAGGCGGTGCGGCAC`	`28 bp deleted → frameshift`
73	`GAGG---------------------------------------------CGGTGCGGCAC`	`45 bp deleted → no frameshift`
60	`GAGGATGACAGCTTAG--------------------------------GCGGTGCGGCAC`	`32 bp deleted → frameshift`
60	`GAGGATGACAGC--------------------------------------GGTGCGGCAC`	`38 bp deleted → frameshift`
47	`GAGGATGACAG-------------------------------------GCGGTGCGGCAC`	`37 bp deleted → frameshift`
46	`GAGGATGACAGC-------------------------------------------GGCAC`	`43 bp deleted → frameshift`
42	`GAGGATGA---------------------------------------GGCGGTGCGGCAC`	`39 bp deleted → no frameshift`
40	`GAGGATGACAGC----------------------------------------------AC`	`46 bp deleted → frameshift`
36	`GAGGA------------------------------------------GGCGGTGCGGCAC`	`42 bp deleted → no frameshift`
36	`GAGG------------------------------------------------TGCGGCAC`	`48 bp deleted → no frameshift`
34	`GAG-------------------------------------------AGGCGGTGCGGCAC`	`43 bp deleted → frameshift`
28	`GAGGATG-----------------------------------------------CGGCAC`	`47 bp deleted → frameshift`
28	`GAGG-----------------------------------------------------CAC`	`53 bp deleted → frameshift`
25	`GAGGATGACA-------------------------------------------------C`	`49 bp deleted → frameshift`
23	`GAGGATGAC---------------------------------------------------`	`51 bp deleted → no frameshift`

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