CRISPOR

DNA break repair outcome predictions

Guide: CGCCGTGTCGACGGCGACCG CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CAGGCGCCGGGTAGCCCCAGCGCCGTGTCGACGGCGACCGCGGAGATGGCAGTGGGGAGCTAGGGTCACCTTGTGATGCG`	(Wild-type sequence)
382	`GTAGCCCCAGCGCCGTGTCGACGG---------AGATGGCAGTGGGGAGCTAGGGTCACC`	`9 bp deleted → no frameshift`
362	`GTAGCCCCAGCGCCGTGTCGACGGCGACCG--GAGATGGCAGTGGGGAGCTAGGGTCACC`	`2 bp deleted → frameshift`
296	`GTAGCCCCAGCGCCGTGTCGACGGCG------GAGATGGCAGTGGGGAGCTAGGGTCACC`	`6 bp deleted → no frameshift`
283	`GTAGCCCCAGCGCCGTGTCGACGGC---------------AGTGGGGAGCTAGGGTCACC`	`15 bp deleted → no frameshift`
219	`GTAGCCCCAGCGCCGTGTCG------------GAGATGGCAGTGGGGAGCTAGGGTCACC`	`12 bp deleted → no frameshift`
211	`GTAGCCCCAGCGCCGTGTCGACGGCGA-------GATGGCAGTGGGGAGCTAGGGTCACC`	`7 bp deleted → frameshift`
191	`GTAGCCCCAGCGCCGTGTCGACGGCGA---------TGGCAGTGGGGAGCTAGGGTCACC`	`9 bp deleted → no frameshift`
170	`GTAGCCCCAGCGCCG-----------------GAGATGGCAGTGGGGAGCTAGGGTCACC`	`17 bp deleted → frameshift`
156	`GTAGCCCCAGCGCCGTGTCGA-------------GATGGCAGTGGGGAGCTAGGGTCACC`	`13 bp deleted → frameshift`
147	`GTAGCCCCAGCG--------------------GAGATGGCAGTGGGGAGCTAGGGTCACC`	`20 bp deleted → frameshift`
141	`GTAGCCCCAGCGCCGTGTCGA---------------TGGCAGTGGGGAGCTAGGGTCACC`	`15 bp deleted → no frameshift`
140	`GTAGCCCCAGCGCCGTGTCGACGG---------------------GGAGCTAGGGTCACC`	`21 bp deleted → no frameshift`
133	`GTAGCCCCAGCGCCGTGTCGACGG----------------------GAGCTAGGGTCACC`	`22 bp deleted → frameshift`
129	`GTAGCCCCAGCGCCGTG---------------------------GGGAGCTAGGGTCACC`	`27 bp deleted → no frameshift`
126	`GTAGCCCCAGCGCCGTGTCGACGG-----------------------AGCTAGGGTCACC`	`23 bp deleted → frameshift`
114	`GTAGCCCCAGCGCCGTGTCGACGGC-------------------------TAGGGTCACC`	`25 bp deleted → frameshift`
106	`GTAGCCCCAGCGCCGTGTCGACGGCGACC-------------------------------`	`31 bp deleted → frameshift`
105	`GTAGCCCCAGCGCCGTGTCGACGGCGA---------------------GCTAGGGTCACC`	`21 bp deleted → no frameshift`
105	`GTAGCCCCAGCGCCGTG---------------------GCAGTGGGGAGCTAGGGTCACC`	`21 bp deleted → no frameshift`
103	`GTAGCCCCAGCGC---------------------------AGTGGGGAGCTAGGGTCACC`	`27 bp deleted → no frameshift`
101	`GTAGCCCCAG--------------------------------TGGGGAGCTAGGGTCACC`	`32 bp deleted → frameshift`
94	`GTAGCCCCAGC-----------------------------AGTGGGGAGCTAGGGTCACC`	`29 bp deleted → frameshift`
89	`GTAGCCCCAGCGCCGTGTCGACGG------------------------------GTCACC`	`30 bp deleted → no frameshift`
86	`GTAGCCCCAGCGCCGTGT-------------------------GGGGAGCTAGGGTCACC`	`25 bp deleted → frameshift`
86	`GTAGCCCCAG-------------------------ATGGCAGTGGGGAGCTAGGGTCACC`	`25 bp deleted → frameshift`
84	`GTAGCCCCAGCGCCGTGTCGACGG-------------------------------TCACC`	`31 bp deleted → frameshift`
77	`GTAGCCCCAGCGCCGTGTCGA---------------------------GCTAGGGTCACC`	`27 bp deleted → no frameshift`
75	`GTAGCCCCAGCGCCGTGTC--------------------------------------ACC`	`38 bp deleted → frameshift`
71	`GTAGCCCCAGC---------------------------------------TAGGGTCACC`	`39 bp deleted → no frameshift`
69	`GTAGC-----------------------------------AGTGGGGAGCTAGGGTCACC`	`35 bp deleted → frameshift`
63	`GTAG-------------------------------ATGGCAGTGGGGAGCTAGGGTCACC`	`31 bp deleted → frameshift`
62	`GTAGCCCCAGCGC-------------------------------------TAGGGTCACC`	`37 bp deleted → frameshift`
52	`GTAGC---------------------------------------------TAGGGTCACC`	`45 bp deleted → no frameshift`
47	`GTAGCCCCAGCGCCGTGTCGAC-------------------------------------C`	`37 bp deleted → frameshift`
45	`GTAG--------------------------------------TGGGGAGCTAGGGTCACC`	`38 bp deleted → frameshift`
40	`GTAGCCCCAGCGCCGT----------------------------------------CACC`	`40 bp deleted → frameshift`
40	`GTAGCCCCAGCGCC----------------------------------------------`	`46 bp deleted → frameshift`
38	`GT-----------------------------------------GGGGAGCTAGGGTCACC`	`41 bp deleted → frameshift`
34	`GTAGCCCCAG-------------------------------------------GGTCACC`	`43 bp deleted → frameshift`
34	`GTAG-------------------------------------------------GGTCACC`	`49 bp deleted → frameshift`
29	`GTAGCCCC----------------------------------------------------`	`52 bp deleted → frameshift`
28	`GTAGCCC-----------------------------------------------------`	`53 bp deleted → frameshift`
26	`GTAGCC------------------------------------------------------`	`54 bp deleted → no frameshift`
25	`GTAGCCCCA-------------------------------------------------CC`	`49 bp deleted → frameshift`
20	`GT------------------------------------------------------CACC`	`54 bp deleted → no frameshift`

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