CRISPOR

DNA break repair outcome predictions

Guide: CGGACAGCCTCTCTGGCCAA GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCTCAGACACCGAATCGGATTTCTATG`	(Wild-type sequence)
423	`GTCCCCTCCGCGGACAGCCTCTCTGGC-------AACCTAGTAGCTCAGACACCGAATCG`	`7 bp deleted → frameshift`
296	`GTCCCCTCCGCGGACAGCCTCTCTGGCCAA------CCTAGTAGCTCAGACACCGAATCG`	`6 bp deleted → no frameshift`
296	`GTCCCCTCCGCGGACAGCCTCTCTGG------GCAACCTAGTAGCTCAGACACCGAATCG`	`6 bp deleted → no frameshift`
242	`GTCCCCTCCGCGGACAGCCTCTCTGGCC----------TAGTAGCTCAGACACCGAATCG`	`10 bp deleted → frameshift`
193	`GTCCCCTCCGCGGACAGCCT-------------------AGTAGCTCAGACACCGAATCG`	`19 bp deleted → frameshift`
179	`GTCCCCTCCGCGGACAGC----------------AACCTAGTAGCTCAGACACCGAATCG`	`16 bp deleted → frameshift`
162	`GTCCCCTCCGCGGACAGCCTCTCTGGC------------------TCAGACACCGAATCG`	`18 bp deleted → no frameshift`
154	`GTCCCCTCCGCGG-------------------GCAACCTAGTAGCTCAGACACCGAATCG`	`19 bp deleted → frameshift`
150	`GTCCCCTCCGCGGACAGCCTCTC------------------------AGACACCGAATCG`	`24 bp deleted → no frameshift`
147	`GTCCCCTCCGCGG--------------------CAACCTAGTAGCTCAGACACCGAATCG`	`20 bp deleted → frameshift`
141	`GTCCCCTCCGCGGACAGCCTCTCT---------------AGTAGCTCAGACACCGAATCG`	`15 bp deleted → no frameshift`
136	`GTCCCCTCCGCGGACAGCCTC--------------------------AGACACCGAATCG`	`26 bp deleted → frameshift`
129	`GTCCCCTCCGCGGACAGC---------------------------TCAGACACCGAATCG`	`27 bp deleted → no frameshift`
128	`GTCCCCTCCGCGGACAGCCTCT-----------------AGTAGCTCAGACACCGAATCG`	`17 bp deleted → frameshift`
126	`GTCCCCTCCGC-----------------------AACCTAGTAGCTCAGACACCGAATCG`	`23 bp deleted → frameshift`
116	`GTCCCCTCCGCGGACAGCCTCTCTGGCCA-------------------GACACCGAATCG`	`19 bp deleted → frameshift`
116	`GTCCCCTCCGCGGACA-------------------ACCTAGTAGCTCAGACACCGAATCG`	`19 bp deleted → frameshift`
109	`GTCCCCTCCGCGGACAGCCTCTCTGGCC--------------------------GAATCG`	`26 bp deleted → frameshift`
101	`GTCCCCTCCGCGGACAG--------------------------------ACACCGAATCG`	`32 bp deleted → frameshift`
101	`GTCCCCT--------------------------------AGTAGCTCAGACACCGAATCG`	`32 bp deleted → frameshift`
99	`GTCCCCTCCGCGGACAGCCTCTCT----------------------CAGACACCGAATCG`	`22 bp deleted → frameshift`
99	`GTCCCCTCCGCGGAC----------------------CTAGTAGCTCAGACACCGAATCG`	`22 bp deleted → frameshift`
99	`GTCCCCTCCGCGGACA------------------------------------CCGAATCG`	`36 bp deleted → no frameshift`
95	`GTCCCCTCCGCGGACAGCCTCTCTGGCCA-----------------------CCGAATCG`	`23 bp deleted → frameshift`
94	`GTCCCCTCC-----------------------------TAGTAGCTCAGACACCGAATCG`	`29 bp deleted → frameshift`
90	`GTCCCCTCCGCGGACAG------------------------TAGCTCAGACACCGAATCG`	`24 bp deleted → no frameshift`
76	`GTCCC---------------------------------TAGTAGCTCAGACACCGAATCG`	`33 bp deleted → no frameshift`
73	`GTCCCCTCCGC----------------------------------TCAGACACCGAATCG`	`34 bp deleted → frameshift`
73	`GTCC----------------------------------TAGTAGCTCAGACACCGAATCG`	`34 bp deleted → frameshift`
71	`GTCCCCTC---------------------------------------AGACACCGAATCG`	`39 bp deleted → no frameshift`
69	`GTCCCCTCCGCGGACAGCC-----------------------------------GAATCG`	`35 bp deleted → frameshift`
63	`GTCCCCTCCG---------------------------------------------AATCG`	`45 bp deleted → no frameshift`
51	`GTCCCCTCCGCGGACAGCCTCTCTGGCCAA---------------------------TCG`	`27 bp deleted → no frameshift`
49	`GTCCCCTCCGCGGACAGCCTCTC------------------------------------G`	`36 bp deleted → no frameshift`
46	`GTCCCCTCCGCG-------------------------------------------AATCG`	`43 bp deleted → frameshift`
45	`GTCCCCTCCGCGGACAGCCTC--------------------------------------G`	`38 bp deleted → frameshift`
45	`GTCCCCTCCGCGGAC--------------------------------------CGAATCG`	`38 bp deleted → frameshift`
40	`GT----------------------------------------AGCTCAGACACCGAATCG`	`40 bp deleted → frameshift`
36	`GTCCCCTCCGCGGA------------------------------------------ATCG`	`42 bp deleted → no frameshift`
36	`GTCCCCTCCGCG------------------------------------------------`	`48 bp deleted → no frameshift`
36	`GTCCCC------------------------------------------------GAATCG`	`48 bp deleted → no frameshift`
34	`GTCCC-------------------------------------------------GAATCG`	`49 bp deleted → frameshift`
33	`GTC--------------------------------------------AGACACCGAATCG`	`44 bp deleted → frameshift`
32	`GTCCCCTCCG--------------------------------------------------`	`50 bp deleted → frameshift`
32	`GTCC--------------------------------------------------GAATCG`	`50 bp deleted → frameshift`
23	`GTCCCCTC---------------------------------------------------G`	`51 bp deleted → no frameshift`
18	`GTC--------------------------------------------------------G`	`56 bp deleted → frameshift`

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