CRISPOR

DNA break repair outcome predictions

Guide: CTGCCTGCTAGTCCATCGAC CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`CGGCGCCCAGCCCTGCAGCACTGCCTGCTAGTCCATCGACCGGCTCCTCCTCCTGGCCTCCCCCGCCACGCTCCCGGGAA`	(Wild-type sequence)
327	`CCCTGCAGCACTGCCTGCTAGTCCATCG----GCTCCTCCTCCTGGCCTCCCCCGCCACG`	`4 bp deleted → frameshift`
261	`CCCTGCAGCACTGCCTGCTAGTCC-------------TCCTCCTGGCCTCCCCCGCCACG`	`13 bp deleted → frameshift`
224	`CCCTGCAGCACTGCCTGCTAGTCC----------------TCCTGGCCTCCCCCGCCACG`	`16 bp deleted → frameshift`
224	`CCCTGCAGCACTGCCTGCT----------------CCTCCTCCTGGCCTCCCCCGCCACG`	`16 bp deleted → frameshift`
193	`CCCTGCAGCACTGCCTGCTAGTCC-------------------TGGCCTCCCCCGCCACG`	`19 bp deleted → frameshift`
191	`CCCTGCAGCACTGCCTGCTAGTCCATC---------CTCCTCCTGGCCTCCCCCGCCACG`	`9 bp deleted → no frameshift`
172	`CCCTGCAGCACTGCCTG----------------------------GCCTCCCCCGCCACG`	`28 bp deleted → frameshift`
166	`CCCTGCAGCACTGCCT----------------------CCTCCTGGCCTCCCCCGCCACG`	`22 bp deleted → frameshift`
164	`CCCTGCAGCACTGCCTGCTAGTCCATC------------CTCCTGGCCTCCCCCGCCACG`	`12 bp deleted → no frameshift`
147	`CCCTGCAGCACTGC--------------------TCCTCCTCCTGGCCTCCCCCGCCACG`	`20 bp deleted → frameshift`
143	`CCCTGCAGCACTGCCT-------------------------CCTGGCCTCCCCCGCCACG`	`25 bp deleted → frameshift`
141	`CCCTGCAGCACTGCCTGCTAGTCCATC---------------CTGGCCTCCCCCGCCACG`	`15 bp deleted → no frameshift`
134	`CCCTGCAGCACTGCCT---------------------------------CCCCCGCCACG`	`33 bp deleted → no frameshift`
129	`CCCTGCAGCACTGCCTGCTAGTCC---------------------------CCCGCCACG`	`27 bp deleted → no frameshift`
120	`CCCTGCAGCACTGCCTGCTAGTCC------------------------TCCCCCGCCACG`	`24 bp deleted → no frameshift`
116	`CCCTGCAGCACTGCCTGCT-------------------CCTCCTGGCCTCCCCCGCCACG`	`19 bp deleted → frameshift`
116	`CCCTGCAGCACTGCCT-------------------CCTCCTCCTGGCCTCCCCCGCCACG`	`19 bp deleted → frameshift`
114	`CCCTGCAGC-------------------------TCCTCCTCCTGGCCTCCCCCGCCACG`	`25 bp deleted → frameshift`
103	`CCCTGCAGCACTGCCTGCTAGTCCATCG---------------------------CCACG`	`27 bp deleted → no frameshift`
101	`CCCTGCAGCACTG--------------------------------GCCTCCCCCGCCACG`	`32 bp deleted → frameshift`
99	`CCCTGCAGCACTGCCTGCT----------------------CCTGGCCTCCCCCGCCACG`	`22 bp deleted → frameshift`
98	`CCCTGCAGCACTGCCTGCTAGTCC----------------------------CCGCCACG`	`28 bp deleted → frameshift`
98	`CCCTGC----------------------------TCCTCCTCCTGGCCTCCCCCGCCACG`	`28 bp deleted → frameshift`
96	`CCCTGCAGCACTGCCTGCTAGTCCA---------------------------------CG`	`33 bp deleted → no frameshift`
95	`CCCTGCAGCACTGCCTGCTAGTCCATC-----------------------CCCCGCCACG`	`23 bp deleted → frameshift`
95	`CCCTGCAGCACT-----------------------CCTCCTCCTGGCCTCCCCCGCCACG`	`23 bp deleted → frameshift`
94	`CCCTG----------------------------------------GCCTCCCCCGCCACG`	`40 bp deleted → frameshift`
94	`CCCTGCAGCACTGCCTGCTAGTCC-----------------------------CGCCACG`	`29 bp deleted → frameshift`
94	`CCCTGCAGCACTGCCTGC-----------------------------CTCCCCCGCCACG`	`29 bp deleted → frameshift`
91	`CCCT----------------------------------CCTCCTGGCCTCCCCCGCCACG`	`34 bp deleted → frameshift`
89	`CCCTGCAGCACTGCCTGCTAGTCC------------------------------GCCACG`	`30 bp deleted → no frameshift`
86	`CCCTGCAGCACTGCCTGCT-------------------------GGCCTCCCCCGCCACG`	`25 bp deleted → frameshift`
81	`CCCTGCAGCACT--------------------------CCTCCTGGCCTCCCCCGCCACG`	`26 bp deleted → frameshift`
80	`CCCTGCAGCACTGCCTGCTAGTCCATCG--------------------------------`	`32 bp deleted → frameshift`
78	`CCCT-------------------------------------CCTGGCCTCCCCCGCCACG`	`37 bp deleted → frameshift`
73	`CCCTGCAGCACTGCC------------------------------------------ACG`	`42 bp deleted → no frameshift`
70	`CCCTGCAGCACTGCCTGCTAGTCCATCGAC-----------------------------G`	`29 bp deleted → frameshift`
70	`CCCTGCAGCACT-----------------------------CCTGGCCTCCCCCGCCACG`	`29 bp deleted → frameshift`
69	`CC-----------------------------------TCCTCCTGGCCTCCCCCGCCACG`	`35 bp deleted → frameshift`
66	`CCCTGCAGCACTGCCTGCT------------------------------CCCCCGCCACG`	`30 bp deleted → no frameshift`
66	`CCCTGCAGCACTGCC------------------------------------CCCGCCACG`	`36 bp deleted → no frameshift`
63	`CCCT-------------------------------CCTCCTCCTGGCCTCCCCCGCCACG`	`31 bp deleted → frameshift`
62	`CCCTGCAGCACTGCC-------------------------------------CCGCCACG`	`37 bp deleted → frameshift`
60	`CCCTGCAGCACTGCCTGC--------------------------------------CACG`	`38 bp deleted → frameshift`
60	`CCCTGCAGCACTGCC--------------------------------------CGCCACG`	`38 bp deleted → frameshift`
60	`CCCTGCAGC--------------------------------------CTCCCCCGCCACG`	`38 bp deleted → frameshift`
60	`CC--------------------------------------TCCTGGCCTCCCCCGCCACG`	`38 bp deleted → frameshift`
56	`CCCTGCAGCACTGCC---------------------------------------GCCACG`	`39 bp deleted → no frameshift`
52	`CCCT---------------------------------------------CCCCCGCCACG`	`45 bp deleted → no frameshift`
51	`CCCTGC-----------------------------------------CTCCCCCGCCACG`	`41 bp deleted → frameshift`
51	`CC-----------------------------------------TGGCCTCCCCCGCCACG`	`41 bp deleted → frameshift`
51	`CCC-------------------------------------------------CCGCCACG`	`49 bp deleted → frameshift`
49	`CCC--------------------------------------------------CGCCACG`	`50 bp deleted → frameshift`
47	`CCCTGCAGCACT-------------------------------------CCCCCGCCACG`	`37 bp deleted → frameshift`
46	`CCC---------------------------------------------------GCCACG`	`51 bp deleted → no frameshift`
45	`CCCTGCAGCAC------------------------------------------------G`	`48 bp deleted → no frameshift`
40	`CC----------------------------------------------TCCCCCGCCACG`	`46 bp deleted → frameshift`
38	`CCCTGCAGC-----------------------------------------------CACG`	`47 bp deleted → frameshift`
36	`CCC------------------------------------------------CCCGCCACG`	`48 bp deleted → no frameshift`
32	`CCCTGC--------------------------------------------------CACG`	`50 bp deleted → frameshift`
29	`CC----------------------------------------------------GCCACG`	`52 bp deleted → frameshift`
26	`CCC------------------------------------------------------ACG`	`54 bp deleted → no frameshift`
25	`CC-------------------------------------------------------ACG`	`55 bp deleted → frameshift`
23	`CCCTGCA---------------------------------------------------CG`	`51 bp deleted → no frameshift`

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