CRISPOR

DNA break repair outcome predictions

Guide: GTAATGACGATTGCAGCAGG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GTGGCGTCACCAGCGGTACTGTAATGACGATTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCT`	(Wild-type sequence)
430	`CAGCGGTACTGTAATGACGATTGCAGCAGG---ATGACAGCTTAGAAAGAAGAGGGCAAT`	`3 bp deleted → no frameshift`
347	`CAGCGGTACTGTAATGACGATTGCAGC--------------TTAGAAAGAAGAGGGCAAT`	`14 bp deleted → frameshift`
288	`CAGCGGTACTGTAATGACGATTGCAGCAG-----------CTTAGAAAGAAGAGGGCAAT`	`11 bp deleted → frameshift`
257	`CAGCGGTACTGTAATGAC--------------------AGCTTAGAAAGAAGAGGGCAAT`	`20 bp deleted → frameshift`
222	`CAGCGGTACTGTAATGACGATTGCAG------GATGACAGCTTAGAAAGAAGAGGGCAAT`	`6 bp deleted → no frameshift`
198	`CAGCGGTACTGTAATGACGAT--------------GACAGCTTAGAAAGAAGAGGGCAAT`	`14 bp deleted → frameshift`
170	`CAGCGGTACTGTAATGACGATTGC-----------------TTAGAAAGAAGAGGGCAAT`	`17 bp deleted → frameshift`
156	`CAGCGGTACTGTAATGACGATTG-------------ACAGCTTAGAAAGAAGAGGGCAAT`	`13 bp deleted → frameshift`
143	`CAGCGGTACTGTAATGACGATTGCAGCAGG-------------------------GCAAT`	`25 bp deleted → frameshift`
134	`CAGCGGTACTGTAATGACGATTGCAGCAG----------------AAAGAAGAGGGCAAT`	`16 bp deleted → frameshift`
128	`CAGCGGTACTGTAATGACGA-----------------CAGCTTAGAAAGAAGAGGGCAAT`	`17 bp deleted → frameshift`
128	`CAGCGGTACTGTAATGA-----------------TGACAGCTTAGAAAGAAGAGGGCAAT`	`17 bp deleted → frameshift`
116	`CAGCGGTACTGTAATGACGATTGCAG-------------------AAAGAAGAGGGCAAT`	`19 bp deleted → frameshift`
111	`CAGCGGTACTGTAATGACGATTGCAGCA------------------------------AT`	`30 bp deleted → no frameshift`
110	`CAGCGGTACTGTAATGACGATTGCAGCAG--------------------AAGAGGGCAAT`	`20 bp deleted → frameshift`
109	`CAGC-------------------------------------TTAGAAAGAAGAGGGCAAT`	`37 bp deleted → frameshift`
109	`CAGCGGTACTGTAATGACGATTGCAGCAGG--------------------------CAAT`	`26 bp deleted → frameshift`
103	`CAGCGG---------------------------ATGACAGCTTAGAAAGAAGAGGGCAAT`	`27 bp deleted → no frameshift`
96	`CAGCGGTACTGTAATGACGATTGCA---------------------------------AT`	`33 bp deleted → no frameshift`
95	`CAGCGGTACTGTAATGACGATTGCAGCAG-----------------------AGGGCAAT`	`23 bp deleted → frameshift`
95	`CAGCGGTACTGTAATGACGATTGCAG-----------------------AAGAGGGCAAT`	`23 bp deleted → frameshift`
86	`CAGCGGTACTG-------------------------ACAGCTTAGAAAGAAGAGGGCAAT`	`25 bp deleted → frameshift`
81	`CAGCGGTACTGTAATGACGATTGCAG--------------------------AGGGCAAT`	`26 bp deleted → frameshift`
81	`CAGCGGTACTGTAATGACGA--------------------------AAGAAGAGGGCAAT`	`26 bp deleted → frameshift`
74	`CAGCGGTACTGTAATGACGATTGCAG----------------------------GGCAAT`	`28 bp deleted → frameshift`
70	`CAGCGGTACTGTAATGA-----------------------------AAGAAGAGGGCAAT`	`29 bp deleted → frameshift`
70	`CAGCGGTAC-----------------------------AGCTTAGAAAGAAGAGGGCAAT`	`29 bp deleted → frameshift`
70	`CAG-----------------------------GATGACAGCTTAGAAAGAAGAGGGCAAT`	`29 bp deleted → frameshift`
70	`CAGCGGTACTGTAATGACGATT---------------------AGAAAGAAGAGGGCAAT`	`21 bp deleted → no frameshift`
66	`CAGCGGTACTGTAATGACGA------------------------------AGAGGGCAAT`	`30 bp deleted → no frameshift`
60	`CAGCGGTACT--------------------------------TAGAAAGAAGAGGGCAAT`	`32 bp deleted → frameshift`
57	`CAGCGGTACTGTAATGACGA---------------------------------GGGCAAT`	`33 bp deleted → no frameshift`
57	`CAGCGGTACTGTAATGA---------------------------------AGAGGGCAAT`	`33 bp deleted → no frameshift`
49	`CAGCGGTACTGTAATGA------------------------------------GGGCAAT`	`36 bp deleted → no frameshift`
42	`CAGCGGTACTGTA-------------------------------GAAAGAAGAGGGCAAT`	`31 bp deleted → frameshift`
38	`CAGCGGTACTGTAA---------------------------------AGAAGAGGGCAAT`	`33 bp deleted → no frameshift`
36	`CAGCGGTACTGTAA----------------------------------GAAGAGGGCAAT`	`34 bp deleted → frameshift`
36	`CAG------------------------------------------AAAGAAGAGGGCAAT`	`42 bp deleted → no frameshift`
34	`CAGCGG-------------------------------------------------GCAAT`	`49 bp deleted → frameshift`
33	`CAGCGGTA------------------------------------GAAAGAAGAGGGCAAT`	`36 bp deleted → no frameshift`
32	`CAGCGG--------------------------------------------------CAAT`	`50 bp deleted → frameshift`
31	`CAGCGGTACTGTAAT---------------------------------------------`	`45 bp deleted → no frameshift`
31	`CAGCGGTACTGTAA-------------------------------------GAGGGCAAT`	`37 bp deleted → frameshift`
30	`CAG----------------------------------------------AAGAGGGCAAT`	`46 bp deleted → frameshift`
28	`CAGCGGTACTGTAATGACGAT---------------------------------------`	`39 bp deleted → no frameshift`
28	`CAGC-----------------------------------------------------AAT`	`53 bp deleted → frameshift`
25	`CAG-------------------------------------------------AGGGCAAT`	`49 bp deleted → frameshift`
23	`CAG---------------------------------------------------GGCAAT`	`51 bp deleted → no frameshift`
18	`CA--------------------------------------------------------AT`	`56 bp deleted → frameshift`

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