CRISPOR

DNA break repair outcome predictions

Guide: CCGCCACGCTCCCGGGAAGT CGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GCTCCTCCTCCTGGCCTCCCCCGCCACGCTCCCGGGAAGTCGGAGGGCCGGCTCGCGGGCTCAGGCAACCGTGGGGCACC`	(Wild-type sequence)
402	`CTGGCCTCCCCCGCCACGCTCCCGG--------AGGGCCGGCTCGCGGGCTCAGGCAACC`	`8 bp deleted → frameshift`
359	`CTGGCCTCCCCCGCCACGCTCCCGG----------------CTCGCGGGCTCAGGCAACC`	`16 bp deleted → frameshift`
352	`CTGGCCTCCCCCGCCACGCTCCCGGGA-------GGGCCGGCTCGCGGGCTCAGGCAACC`	`7 bp deleted → frameshift`
346	`CTGGCCTCCCCCGCCACGCTCCCGGG-----------CCGGCTCGCGGGCTCAGGCAACC`	`11 bp deleted → frameshift`
253	`CTGGCCTCCCCCGCCACGCTCCCGGG-----------------------CTCAGGCAACC`	`23 bp deleted → frameshift`
242	`CTGGCCTCCCCCGCCACGCTCCCGGG----------GCCGGCTCGCGGGCTCAGGCAACC`	`10 bp deleted → frameshift`
222	`CTGGCCTCCCCCGCCACGCTCCCGGGAAG------GGCCGGCTCGCGGGCTCAGGCAACC`	`6 bp deleted → no frameshift`
221	`CTGGCCTCCCCCGCCACGCTC-----------------------GCGGGCTCAGGCAACC`	`23 bp deleted → frameshift`
219	`CTGGCCTCCCCCGCCACGCTCCCGG------------CCGGCTCGCGGGCTCAGGCAACC`	`12 bp deleted → no frameshift`
198	`CTGGCCTCCCCCGCCACG--------------GAGGGCCGGCTCGCGGGCTCAGGCAACC`	`14 bp deleted → frameshift`
188	`CTGGCCTCCCCCGCCACGCTCCCGGG---------------CTCGCGGGCTCAGGCAACC`	`15 bp deleted → no frameshift`
180	`CTGGCCTCCCCCGCC------------------------GGCTCGCGGGCTCAGGCAACC`	`24 bp deleted → no frameshift`
170	`CTGGCCTCCCCCGCCACGCTCC-----------------GGCTCGCGGGCTCAGGCAACC`	`17 bp deleted → frameshift`
155	`CTGGCCTCCCCCGCCACGC---------------------------GGGCTCAGGCAACC`	`27 bp deleted → no frameshift`
155	`CTGGCCTCCCCCG---------------------------GCTCGCGGGCTCAGGCAACC`	`27 bp deleted → no frameshift`
154	`CTGGCCTCCCCCGCCACGC-------------------CGGCTCGCGGGCTCAGGCAACC`	`19 bp deleted → frameshift`
154	`CTGGCCTCCCCCG-------------------GAGGGCCGGCTCGCGGGCTCAGGCAACC`	`19 bp deleted → frameshift`
153	`CTGGCC---------------------------------GGCTCGCGGGCTCAGGCAACC`	`33 bp deleted → no frameshift`
148	`CTGGCCTCCCCCGCCACGCTC-------------------------------AGGCAACC`	`31 bp deleted → frameshift`
140	`CTGGCCTCCCCCGCCACGCTCCCG---------------------CGGGCTCAGGCAACC`	`21 bp deleted → no frameshift`
133	`CTGGCCTCCCCCGCCACGCTCCCGGG----------------------GCTCAGGCAACC`	`22 bp deleted → frameshift`
133	`CTGGCCTCCCCCGCCACG----------------------GCTCGCGGGCTCAGGCAACC`	`22 bp deleted → frameshift`
121	`CTGGCCTCCCCCGC--------------------------------GGGCTCAGGCAACC`	`32 bp deleted → frameshift`
120	`CTGGCCTCCCCCGCCACGCTCCCGG------------------------CTCAGGCAACC`	`24 bp deleted → no frameshift`
98	`CTGGCCTCCCCCGC----------------------------TCGCGGGCTCAGGCAACC`	`28 bp deleted → frameshift`
98	`CTGGCCTCCCC----------------------------GGCTCGCGGGCTCAGGCAACC`	`28 bp deleted → frameshift`
94	`CTGGC-------------------------------------TCGCGGGCTCAGGCAACC`	`37 bp deleted → frameshift`
94	`CTGGCCTCCCCCGCCACGCTCCCGGG-----------------------------CAACC`	`29 bp deleted → frameshift`
94	`CTGGCCTCCCCCGCCACG-----------------------------GGCTCAGGCAACC`	`29 bp deleted → frameshift`
94	`CTGGCCTCCC-----------------------------GGCTCGCGGGCTCAGGCAACC`	`29 bp deleted → frameshift`
94	`CTGG-----------------------------AGGGCCGGCTCGCGGGCTCAGGCAACC`	`29 bp deleted → frameshift`
89	`CTGGCCTCCCCCGCCACGCTCCCGG------------------------------CAACC`	`30 bp deleted → no frameshift`
89	`CTGGCCTCC------------------------------GGCTCGCGGGCTCAGGCAACC`	`30 bp deleted → no frameshift`
86	`CTGGCCTCCCCCGCCACGCTCCCGGGAAG-------------------------GCAACC`	`25 bp deleted → frameshift`
82	`CTGGCCTC------------------------------------GCGGGCTCAGGCAACC`	`36 bp deleted → no frameshift`
80	`CTGG--------------------------------GCCGGCTCGCGGGCTCAGGCAACC`	`32 bp deleted → frameshift`
73	`CTGGCCTCCCCCG----------------------------------GGCTCAGGCAACC`	`34 bp deleted → frameshift`
66	`CTGGCCTCCCCCGC------------------------------------TCAGGCAACC`	`36 bp deleted → no frameshift`
63	`CTGGC---------------------------------------------TCAGGCAACC`	`45 bp deleted → no frameshift`
62	`CTGGCCTCCCCCGCCACGCTCCC-------------------------------------`	`37 bp deleted → frameshift`
62	`CTGGCCTCCCCCGCCACGC-------------------------------------AACC`	`37 bp deleted → frameshift`
60	`CTGGCCTCCCCCGCCACGCTCC--------------------------------------`	`38 bp deleted → frameshift`
55	`CTGGCCTC--------------------------------------------AGGCAACC`	`44 bp deleted → frameshift`
51	`CTGGC-----------------------------------------GGGCTCAGGCAACC`	`41 bp deleted → frameshift`
48	`CTGGCCTCCCCCGC------------------------------------------AACC`	`42 bp deleted → no frameshift`
47	`CTGGCCTCCCCCGCCA-------------------------------------GGCAACC`	`37 bp deleted → frameshift`
46	`CTGGC---------------------------------------------------AACC`	`51 bp deleted → no frameshift`
44	`CTGGCCTCCCCCGCCACGCTCCCGGGAA------------------------------CC`	`30 bp deleted → no frameshift`
44	`CTGG--------------------------------------------GCTCAGGCAACC`	`44 bp deleted → frameshift`
42	`CTGGCCTCCCCCGCC---------------------------------------------`	`45 bp deleted → no frameshift`
38	`CTGGCCTCCCCCGCCA-----------------------------------------ACC`	`41 bp deleted → frameshift`
38	`CT-----------------------------------------CGCGGGCTCAGGCAACC`	`41 bp deleted → frameshift`
36	`CTGGCCTCCCCCGCCAC------------------------------------------C`	`42 bp deleted → no frameshift`
36	`CTGGCCTCCCCC------------------------------------------------`	`48 bp deleted → no frameshift`
34	`CTGGCCTCCCC-------------------------------------------------`	`49 bp deleted → frameshift`
32	`CTGGCCTCCC--------------------------------------------------`	`50 bp deleted → frameshift`
31	`CTGGCCTCC---------------------------------------------------`	`51 bp deleted → no frameshift`
26	`CTGGCC------------------------------------------------------`	`54 bp deleted → no frameshift`
25	`CT-------------------------------------------------CAGGCAACC`	`49 bp deleted → frameshift`

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