CRISPOR

DNA break repair outcome predictions

Guide: CTTAGAAAGAAGAGGGCAAT GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCT`	(Wild-type sequence)
536	`AGGATGACAGCTTAGAAAGAAGAGGGC--------TTCCTCCCAGAGGCGGTGCGGCACA`	`8 bp deleted → frameshift`
423	`AGGATGACAGCTTAGAAAGAAGAGGG-------GCTTCCTCCCAGAGGCGGTGCGGCACA`	`7 bp deleted → frameshift`
296	`AGGATGACAGCTTAGAAAGAAGAGGG------GGCTTCCTCCCAGAGGCGGTGCGGCACA`	`6 bp deleted → no frameshift`
255	`AGGATGACAGCTTAGAAAGAAGAGG---------CTTCCTCCCAGAGGCGGTGCGGCACA`	`9 bp deleted → no frameshift`
253	`AGGATGACAGCTTAGAAAGAAGAGG-----------------------CGGTGCGGCACA`	`23 bp deleted → frameshift`
199	`AGGATGACAGCTTAGAAAGAAGAGGGC----------------------GGTGCGGCACA`	`22 bp deleted → frameshift`
180	`AGGATGACAGCTT------------------------CCTCCCAGAGGCGGTGCGGCACA`	`24 bp deleted → no frameshift`
156	`AGGATGACAGCTTAGAAAGAAGAGGGCA------------------------------CA`	`30 bp deleted → no frameshift`
134	`AGGATGACAGCTTAGAAAGAAGAGGGCA----------------GAGGCGGTGCGGCACA`	`16 bp deleted → frameshift`
114	`AGGATGACAGCTTAGAAAGAAGAGGG-------------------------TGCGGCACA`	`25 bp deleted → frameshift`
109	`AGGATGACAGCTTAGAAAGAAGAGG--------------------------TGCGGCACA`	`26 bp deleted → frameshift`
109	`AGGATGACAGCTTAGAAAGA--------------------------GGCGGTGCGGCACA`	`26 bp deleted → frameshift`
105	`AGGATGACAGCTTAGAAAGAAGAG---------------------AGGCGGTGCGGCACA`	`21 bp deleted → no frameshift`
103	`AGGATGACAGCTTAGAAAGAAGAGGGC---------------------------GGCACA`	`27 bp deleted → no frameshift`
94	`AGG-----------------------------GGCTTCCTCCCAGAGGCGGTGCGGCACA`	`29 bp deleted → frameshift`
89	`AGGATGACAGCTTAGA------------------------------GGCGGTGCGGCACA`	`30 bp deleted → no frameshift`
89	`AGG------------------------------GCTTCCTCCCAGAGGCGGTGCGGCACA`	`30 bp deleted → no frameshift`
87	`AGGATGACAG-----------------------------------AGGCGGTGCGGCACA`	`35 bp deleted → frameshift`
86	`AGGATGACAGCTTAGAAAGAAG-------------------------GCGGTGCGGCACA`	`25 bp deleted → frameshift`
84	`AGGATGACAGCTTAGAAAGAAGAGG-------------------------------CACA`	`31 bp deleted → frameshift`
84	`AGG-------------------------------CTTCCTCCCAGAGGCGGTGCGGCACA`	`31 bp deleted → frameshift`
74	`AGGATGACAGCTTAGAAAG----------------------------GCGGTGCGGCACA`	`28 bp deleted → frameshift`
74	`AGGATGACAGCT----------------------------CCCAGAGGCGGTGCGGCACA`	`28 bp deleted → frameshift`
60	`AGGATGACAGCTTAGAAAGAAGAGGGCA--------------------------------`	`32 bp deleted → frameshift`
60	`AGGATGACAGCTTAG--------------------------------GCGGTGCGGCACA`	`32 bp deleted → frameshift`
60	`AGGATGACAGC--------------------------------------GGTGCGGCACA`	`38 bp deleted → frameshift`
52	`AGG---------------------------------------------CGGTGCGGCACA`	`45 bp deleted → no frameshift`
47	`AGGATGACAG-------------------------------------GCGGTGCGGCACA`	`37 bp deleted → frameshift`
46	`AGGATGACAGC-------------------------------------------GGCACA`	`43 bp deleted → frameshift`
42	`AGGATGA---------------------------------------GGCGGTGCGGCACA`	`39 bp deleted → no frameshift`
40	`AGGATGACAGC----------------------------------------------ACA`	`46 bp deleted → frameshift`
36	`AGGA------------------------------------------GGCGGTGCGGCACA`	`42 bp deleted → no frameshift`
36	`AGG------------------------------------------------TGCGGCACA`	`48 bp deleted → no frameshift`
34	`AG-------------------------------------------AGGCGGTGCGGCACA`	`43 bp deleted → frameshift`
31	`AGGATGACA---------------------------------------------------`	`51 bp deleted → no frameshift`
28	`AGGATG-----------------------------------------------CGGCACA`	`47 bp deleted → frameshift`
28	`AGG-----------------------------------------------------CACA`	`53 bp deleted → frameshift`
25	`AGGATGACA-------------------------------------------------CA`	`49 bp deleted → frameshift`

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