CRISPOR

DNA break repair outcome predictions

Guide: GCACCCCAGACTGCGCCACC GGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TGAAGAAATCGAGGTGAGCTGCACCCCAGACTGCGCCACCGGGAACGCCGAGTACCAGCACAGCAAAGGTAGCCACCTTG`	(Wild-type sequence)
439	`GAGGTGAGCTGCACCCCAGACTGCGCC------------GAGTACCAGCACAGCAAAGGT`	`12 bp deleted → no frameshift`
255	`GAGGTGAGCTGCACCCCAGACTGCGCCACC---------GAGTACCAGCACAGCAAAGGT`	`9 bp deleted → no frameshift`
224	`GAGGTGAGCTGCACCCCAGACTGCGCCACC----------------AGCACAGCAAAGGT`	`16 bp deleted → frameshift`
211	`GAGGTGAGCTGCACCCCAGACTGCGCCAC-------GCCGAGTACCAGCACAGCAAAGGT`	`7 bp deleted → frameshift`
198	`GAGGTGAGCTGCACCCCAGACTGC--------------CGAGTACCAGCACAGCAAAGGT`	`14 bp deleted → frameshift`
193	`GAGGTGAGCTGCACCCCAGACTGCGCCA-------------------GCACAGCAAAGGT`	`19 bp deleted → frameshift`
188	`GAGGTGAGCTGCACCCCAGACTGCG---------------AGTACCAGCACAGCAAAGGT`	`15 bp deleted → no frameshift`
166	`GAGGTGAGCTGCACCCCAGACTGCGCCAC----------------------AGCAAAGGT`	`22 bp deleted → frameshift`
164	`GAGGTGAGCTGCACCCCAG-----------------------------CACAGCAAAGGT`	`29 bp deleted → frameshift`
149	`GAGGTGAGCTGCACCCCAGA--------------ACGCCGAGTACCAGCACAGCAAAGGT`	`14 bp deleted → frameshift`
141	`GAGGTGAGCTGCACCCCAGAC---------------GCCGAGTACCAGCACAGCAAAGGT`	`15 bp deleted → no frameshift`
133	`GAGGTGAGCTGCACCCC----------------------GAGTACCAGCACAGCAAAGGT`	`22 bp deleted → frameshift`
126	`GAGGTGAGCTGCACCCCAGACTGCGC-----------------------ACAGCAAAGGT`	`23 bp deleted → frameshift`
126	`GAGGTGAGCTGCACCC-----------------------GAGTACCAGCACAGCAAAGGT`	`23 bp deleted → frameshift`
120	`GAGGTGAGCTGCACC------------------------GAGTACCAGCACAGCAAAGGT`	`24 bp deleted → no frameshift`
114	`GAGGTGAGCTGCACCCCAGACTGC-------------------------ACAGCAAAGGT`	`25 bp deleted → frameshift`
109	`GAGGTGAGCTGCAC-------------------------------------AGCAAAGGT`	`37 bp deleted → frameshift`
109	`GAGGTGAGCTGC--------------------------CGAGTACCAGCACAGCAAAGGT`	`26 bp deleted → frameshift`
106	`GAGGTGAGCTGCACC-------------------------------AGCACAGCAAAGGT`	`31 bp deleted → frameshift`
105	`GAGGTGAGCTGCACCCCAGA---------------------GTACCAGCACAGCAAAGGT`	`21 bp deleted → no frameshift`
99	`GAGGTGAGCTGCAC----------------------GCCGAGTACCAGCACAGCAAAGGT`	`22 bp deleted → frameshift`
98	`GAGGTGAGCTGCACCCCAGACTGCGC----------------------------AAAGGT`	`28 bp deleted → frameshift`
98	`GAGG----------------------------GAACGCCGAGTACCAGCACAGCAAAGGT`	`28 bp deleted → frameshift`
95	`GAGGTGAGCTGCACCCCAG-----------------------TACCAGCACAGCAAAGGT`	`23 bp deleted → frameshift`
94	`GAGGTGAGC-----------------------------CGAGTACCAGCACAGCAAAGGT`	`29 bp deleted → frameshift`
94	`GAGG-----------------------------AACGCCGAGTACCAGCACAGCAAAGGT`	`29 bp deleted → frameshift`
91	`GAGGTGAGCTGCACCCCAG----------------------------------CAAAGGT`	`34 bp deleted → frameshift`
91	`GAGGTGAG----------------------------------TACCAGCACAGCAAAGGT`	`34 bp deleted → frameshift`
90	`GAGGTGAGCTGCACCCCAGACTGCGCCA------------------------GCAAAGGT`	`24 bp deleted → no frameshift`
90	`GAGGTGAGCTGCACCCCAGAC------------------------CAGCACAGCAAAGGT`	`24 bp deleted → no frameshift`
89	`GAGGTGAGCTGCACCCCAGACTGC------------------------------AAAGGT`	`30 bp deleted → no frameshift`
89	`GAGGTGAGCTGCACCC------------------------------AGCACAGCAAAGGT`	`30 bp deleted → no frameshift`
77	`GAGGTGAGCTGCACCCCAGACTGCGCCA---------------------------AAGGT`	`27 bp deleted → no frameshift`
77	`GAGGTGA---------------------------ACGCCGAGTACCAGCACAGCAAAGGT`	`27 bp deleted → no frameshift`
71	`GAG---------------------------------------TACCAGCACAGCAAAGGT`	`39 bp deleted → no frameshift`
67	`GAGGTGAGC----------------------------------------ACAGCAAAGGT`	`40 bp deleted → frameshift`
66	`GAGGTGAGCTGCACCCCAGAC------------------------------AGCAAAGGT`	`30 bp deleted → no frameshift`
61	`GAGGTGAGCTGCA------------------------------------------AAGGT`	`42 bp deleted → no frameshift`
60	`GAGGTGAGCTGCACCCCA--------------------------------CAGCAAAGGT`	`32 bp deleted → frameshift`
60	`GA--------------------------------ACGCCGAGTACCAGCACAGCAAAGGT`	`32 bp deleted → frameshift`
54	`GAGGTGAGCTGCA----------------------------------GCACAGCAAAGGT`	`34 bp deleted → frameshift`
52	`GAGGTGAGC---------------------------------------------AAAGGT`	`45 bp deleted → no frameshift`
47	`GAGGTGAGCTGCACCCCA-------------------------------------AAGGT`	`37 bp deleted → frameshift`
45	`GAGGT--------------------------------------ACCAGCACAGCAAAGGT`	`38 bp deleted → frameshift`
42	`GAGGTGAGCTGCACCCCAG---------------------------------------GT`	`39 bp deleted → no frameshift`
42	`GAGGTGAGCTGCA---------------------------------------GCAAAGGT`	`39 bp deleted → no frameshift`
38	`GAGGT-------------------------------------------------------`	`55 bp deleted → frameshift`
31	`GAG---------------------------------------------CACAGCAAAGGT`	`45 bp deleted → no frameshift`
24	`GAGGTGAG--------------------------------------------------GT`	`50 bp deleted → frameshift`
24	`GAG--------------------------------------------------CAAAGGT`	`50 bp deleted → frameshift`

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