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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314132_F | TCGTCGGCAGCGTCGTCGATGGACTAGCAGGCAG | 60.2 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314132_R | GTCTCGTGGGCTCGGAGCTACTAGGTTGCCCTTGG | 59.0 | 1.00 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327975_F | TCGTCGGCAGCGTCTGGCAGGAAGGGAAGAGAGA | 59.8 | 0.71 |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327975_R | GTCTCGTGGGCTCGGGCTTAGCCAAGGAAGCACCA | 60.6 | 0.71 |
| mm2_intergenic_Zmynd8|Gm22692_chr2_165906507_F | Primer3: not found at this Tm | N.d. | 0.63 |
| mm2_intergenic_Zmynd8|Gm22692_chr2_165906507_R | Primer3: not found at this Tm | N.d. | 0.63 |
| mm4_intergenic_Gm25644|Rpl31-ps18_chr19_51269487_F | Primer3: not found at this Tm | N.d. | 0.57 |
| mm4_intergenic_Gm25644|Rpl31-ps18_chr19_51269487_R | Primer3: not found at this Tm | N.d. | 0.57 |
| mm4_intergenic_1700029N11Rik|Jarid2_chr13_44500238_F | TCGTCGGCAGCGTCAGTGGAAGCACTGACAACCA | 59.4 | 0.48 |
| mm4_intergenic_1700029N11Rik|Jarid2_chr13_44500238_R | GTCTCGTGGGCTCGGTAACCGCCATGACCATAGCC | 59.8 | 0.48 |
| mm2_intron_Scp2_chr4_108045347_F | Primer3: not found at this Tm | N.d. | 0.43 |
| mm2_intron_Scp2_chr4_108045347_R | Primer3: not found at this Tm | N.d. | 0.43 |
| mm4_intron_1700016C15Rik_chr1_177744442_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_1700016C15Rik_chr1_177744442_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Cadm2|Gm15828_chr16_67242555_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Cadm2|Gm15828_chr16_67242555_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intron_Cntn5_chr9_10786995_F | TCGTCGGCAGCGTCACATGATGAGAAAAGGACTGTGC | 59.2 | 0.38 |
| mm4_intron_Cntn5_chr9_10786995_R | GTCTCGTGGGCTCGGTGGAATGAACCCAGTCTGGC | 59.9 | 0.38 |
| mm4_intergenic_Ptgs2|Gm26687_chr1_150145765_F | Primer3: not found at this Tm | N.d. | 0.35 |
| mm4_intergenic_Ptgs2|Gm26687_chr1_150145765_R | Primer3: not found at this Tm | N.d. | 0.35 |
| mm3_intron_Tmem55a_chr4_14883738_F | TCGTCGGCAGCGTCATGTAGAGCCATTTTATAAGGATGC | 57.3 | 0.31 |
| mm3_intron_Tmem55a_chr4_14883738_R | GTCTCGTGGGCTCGGAGAACTGAAGTGCATTGGGT | 57.3 | 0.31 |
| mm3_exon_Pkd1_chr17_24571507_F | TCGTCGGCAGCGTCCTCGAGGCCTTTCACCCATT | 60.0 | 0.30 |
| mm3_exon_Pkd1_chr17_24571507_R | GTCTCGTGGGCTCGGTTCATCCGCTCCACAGTGAC | 60.0 | 0.30 |
| mm4_intergenic_Kcnk18|Slc18a2_chr19_59259925_F | TCGTCGGCAGCGTCCACCACAAGGCTGCTCCTAG | 60.3 | 0.29 |
| mm4_intergenic_Kcnk18|Slc18a2_chr19_59259925_R | GTCTCGTGGGCTCGGGAGGGGTCAGAGCTGTGTTC | 60.0 | 0.29 |
| mm3_exon_Lrrc24_chr15_76718365_F | TCGTCGGCAGCGTCCTGGACCAAGCCCACAAATG | 59.3 | 0.28 |
| mm3_exon_Lrrc24_chr15_76718365_R | GTCTCGTGGGCTCGGCACAACAATACCCTGCGTGC | 60.1 | 0.28 |
| mm4_intergenic_Gm22235|Epas1_chr17_86740034_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm22235|Epas1_chr17_86740034_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm14168|Gm14169_chr2_156585165_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm14168|Gm14169_chr2_156585165_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_9330198N18Rik|Gm14488_chr2_30707709_F | TCGTCGGCAGCGTCTGCTCGCAGAAGGATGTTGA | 59.6 | 0.26 |
| mm4_intergenic_9330198N18Rik|Gm14488_chr2_30707709_R | GTCTCGTGGGCTCGGGGGTAGCTGCGCAAGAGG | 60.5 | 0.26 |
| mm4_exon_9330160F10Rik/2310047M10Rik_chr11_69059149_F | TCGTCGGCAGCGTCACGTCTGATTCTTTCACCTATGGA | 59.5 | 0.26 |
| mm4_exon_9330160F10Rik/2310047M10Rik_chr11_69059149_R | GTCTCGTGGGCTCGGGCTTTTGTCACTCTTCGGCC | 59.7 | 0.26 |
| mm4_exon_Phyhip_chr14_70468699_F | TCGTCGGCAGCGTCGATGGGCCAAGAGATCTGGG | 59.8 | 0.25 |
| mm4_exon_Phyhip_chr14_70468699_R | GTCTCGTGGGCTCGGGGACAGAAGACAAGGCAGCA | 60.2 | 0.25 |
| mm4_intron_Col2a1_chr15_98003910_F | TCGTCGGCAGCGTCGGGGAGAGCCGAGTTTCAAA | 59.9 | 0.25 |
| mm4_intron_Col2a1_chr15_98003910_R | GTCTCGTGGGCTCGGTGGGTTGAAATCTGAGGCCA | 59.2 | 0.25 |
| mm4_intron_Pced1b_chr15_97379606_F | TCGTCGGCAGCGTCTGAAGGTGGCCATGACAGTC | 59.9 | 0.24 |
| mm4_intron_Pced1b_chr15_97379606_R | GTCTCGTGGGCTCGGGGGAGCTGGTCCTACAGAGA | 60.0 | 0.24 |
| mm4_intron_Ift122_chr6_115897458_F | TCGTCGGCAGCGTCTGCTTGGAATACAGCAGCCA | 59.9 | 0.24 |
| mm4_intron_Ift122_chr6_115897458_R | GTCTCGTGGGCTCGGACGTTGCACAAGATCAGCTG | 59.1 | 0.24 |
| mm4_intergenic_Gm4294|Gm26171_chr12_12024003_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Gm4294|Gm26171_chr12_12024003_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Por_chr5_135732612_F | TCGTCGGCAGCGTCAAGATCAGGTGCCTGCTTCC | 60.0 | 0.23 |
| mm4_intron_Por_chr5_135732612_R | GTCTCGTGGGCTCGGACGGGAGTGCAAGAGTCAAG | 59.9 | 0.23 |
| mm4_intron_Prickle2_chr6_92706047_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Prickle2_chr6_92706047_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_1700116B05Rik|Gm24925_chr16_50848039_F | TCGTCGGCAGCGTCACATCTAGGGGCTGGCTGTA | 60.0 | 0.19 |
| mm4_intergenic_1700116B05Rik|Gm24925_chr16_50848039_R | GTCTCGTGGGCTCGGCCAGCAAGAAGAGCATGGGA | 60.0 | 0.19 |
| mm4_intergenic_Btbd1|Gm10160_chr7_81851432_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Btbd1|Gm10160_chr7_81851432_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Mill2|Pglyrp1_chr7_18877909_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intergenic_Mill2|Pglyrp1_chr7_18877909_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_exon_Dffa_chr4_149104384_F | TCGTCGGCAGCGTCAGTCTCGAGGAGCTGAGGAG | 60.1 | 0.15 |
| mm4_exon_Dffa_chr4_149104384_R | GTCTCGTGGGCTCGGTCAATCACTGGACCCACGTG | 59.9 | 0.15 |
| mm4_intron_Hdac4_chr1_91958283_F | TCGTCGGCAGCGTCATCAACAGGACAAGGCCCAG | 59.9 | 0.15 |
| mm4_intron_Hdac4_chr1_91958283_R | GTCTCGTGGGCTCGGGTTCTGGGGAGAGCATGCAT | 60.1 | 0.15 |
| mm4_intergenic_Gm24908|Gm11836_chr4_14413428_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Gm24908|Gm11836_chr4_14413428_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Ncoa1|Gm3625_chr12_4477296_F | Primer3: not found at this Tm | N.d. | 0.14 |
| mm3_intergenic_Ncoa1|Gm3625_chr12_4477296_R | Primer3: not found at this Tm | N.d. | 0.14 |
| mm4_intergenic_Fnta|Hook3_chr8_26015616_F | TCGTCGGCAGCGTCCAGCAGAGGTGGTGGTGG | 59.9 | 0.14 |
| mm4_intergenic_Fnta|Hook3_chr8_26015616_R | GTCTCGTGGGCTCGGCCTCTACCCCACGCCTCTAT | 60.1 | 0.14 |
| mm4_intergenic_Gimap6|RP24-315D19.2/Gimap7_chr6_48708257_F | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Gimap6|RP24-315D19.2/Gimap7_chr6_48708257_R | Primer3: not found at this Tm | N.d. | 0.13 |
| mm4_intergenic_Qprt|Spn_chr7_127129624_F | TCGTCGGCAGCGTCAGCCTAGTCATTTGCGCCAT | 60.1 | 0.12 |
| mm4_intergenic_Qprt|Spn_chr7_127129624_R | GTCTCGTGGGCTCGGACCAGACTCCTCCTCAACCA | 59.8 | 0.12 |
| mm4_exon_Gramd2_chr9_59680148_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_exon_Gramd2_chr9_59680148_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_2210404O07Rik|Nfic_chr10_81395463_F | TCGTCGGCAGCGTCCTTGGCCACTGAGAGCTCTG | 60.3 | 0.11 |
| mm4_intergenic_2210404O07Rik|Nfic_chr10_81395463_R | GTCTCGTGGGCTCGGCTCCACAGCATTGCAGAGGA | 60.0 | 0.11 |
| mm4_intergenic_Jag1|Gm14055_chr2_137250256_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Jag1|Gm14055_chr2_137250256_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Atp13a2_chr4_140986952_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Atp13a2_chr4_140986952_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Hydin_chr8_110594529_F | TCGTCGGCAGCGTCTTCCCCATCTCCAATTGCCC | 60.0 | 0.11 |
| mm4_intron_Hydin_chr8_110594529_R | GTCTCGTGGGCTCGGTGGGCATGAGGAGAAAGCAG | 60.0 | 0.11 |
| mm4_intron_Lhfp_chr3_53170610_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intron_Lhfp_chr3_53170610_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_exon_Ttyh1_chr7_4133237_F | TCGTCGGCAGCGTCAGGTCAGCAGGGGAGAGAG | 59.9 | 0.10 |
| mm4_exon_Ttyh1_chr7_4133237_R | GTCTCGTGGGCTCGGATGGAAACGAGTCAGGAGGC | 59.7 | 0.10 |
| mm4_intergenic_Gm23788|Gm26974_chr7_123953152_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gm23788|Gm26974_chr7_123953152_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Rab37_chr11_115154470_F | TCGTCGGCAGCGTCCATAACCCCTGGGCTCATCC | 59.8 | 0.10 |
| mm4_intron_Rab37_chr11_115154470_R | GTCTCGTGGGCTCGGAGTGTGGCAGAAGGAACTGG | 59.8 | 0.10 |
| mm3_intergenic_Mir674|Rap1a-ps2_chr2_117218088_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_intergenic_Mir674|Rap1a-ps2_chr2_117218088_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intron_Spef2_chr15_9688079_F | TCGTCGGCAGCGTCTGAAAAGGCAGGACTGAAGCA | 60.1 | 0.10 |
| mm4_intron_Spef2_chr15_9688079_R | GTCTCGTGGGCTCGGTTCCCTACAACCCCTCCACT | 59.8 | 0.10 |
| mm4_intergenic_Ifltd1|Gm25373_chr6_145460428_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Ifltd1|Gm25373_chr6_145460428_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Zfp191_chr18_24011970_F | TCGTCGGCAGCGTCCACGGCGTCCTTTTCCTGTA | 60.3 | 0.09 |
| mm4_exon_Zfp191_chr18_24011970_R | GTCTCGTGGGCTCGGCTGACGCCGTGTTCCTTTTG | 60.0 | 0.09 |
| mm4_intergenic_Gm22895|Gm24713_chr15_17980115_F | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_intergenic_Gm22895|Gm24713_chr15_17980115_R | Primer3: not found at this Tm | N.d. | 0.09 |
| mm4_exon_Ipp_chr4_116507581_F | TCGTCGGCAGCGTCCGCAGTCACCTGTCACCAAA | 60.8 | 0.09 |
| mm4_exon_Ipp_chr4_116507581_R | GTCTCGTGGGCTCGGTGAGAAACACAGACCGCACG | 60.8 | 0.09 |
| mm4_exon_Fmn2_chr1_174502396_F | TCGTCGGCAGCGTCCGAGAGGATGTGCTGGACTC | 59.8 | 0.09 |
| mm4_exon_Fmn2_chr1_174502396_R | GTCTCGTGGGCTCGGTCGAAAGGGTCAGCACACTC | 59.9 | 0.09 |
| mm4_intergenic_Gm13816|Gm13791_chr2_92807509_F | TCGTCGGCAGCGTCTCGCTTTCACATCTGGCTCT | 59.3 | 0.09 |
| mm4_intergenic_Gm13816|Gm13791_chr2_92807509_R | GTCTCGTGGGCTCGGCTTCCCTTGCCCTAGTCACC | 59.7 | 0.09 |
| mm4_exon_Cnp_chr11_100576059_F | TCGTCGGCAGCGTCACCTTACAACCCAGCTGTTGA | 59.5 | 0.08 |
| mm4_exon_Cnp_chr11_100576059_R | GTCTCGTGGGCTCGGGACCGCTTGTCAGTTGAGGA | 59.9 | 0.08 |
| mm4_intergenic_Psma6|Nfkbia_chr12_55449056_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Psma6|Nfkbia_chr12_55449056_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm11958|Gm24803_chr11_4309191_F | TCGTCGGCAGCGTCACAAGGCTCCCCACATTTCT | 59.2 | 0.08 |
| mm4_intergenic_Gm11958|Gm24803_chr11_4309191_R | GTCTCGTGGGCTCGGGAGCCCTGCTGTCTGGAAAT | 60.0 | 0.08 |
| mm4_intergenic_Gm25895|Samt1_chrX_153971956_F | TCGTCGGCAGCGTCAGATGTGGGCAGTGGACATG | 60.0 | 0.08 |
| mm4_intergenic_Gm25895|Samt1_chrX_153971956_R | GTCTCGTGGGCTCGGATGCTTGTCTCTCGAGGCAC | 60.1 | 0.08 |
| mm4_intron_Zbtb7a_chr10_81142765_F | TCGTCGGCAGCGTCCAAAGGGACCTGGATCACCC | 60.0 | 0.08 |
| mm4_intron_Zbtb7a_chr10_81142765_R | GTCTCGTGGGCTCGGGACCATGTGGGCAGACAAGA | 59.9 | 0.08 |
| mm4_intergenic_RP23-218B17.5/Gm13415|Gm13415_chr2_24485383_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_RP23-218B17.5/Gm13415|Gm13415_chr2_24485383_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_RP23-302F9.3_chr8_87235431_F | TCGTCGGCAGCGTCCCCAGTGCAGAACTCTCCTC | 59.7 | 0.07 |
| mm4_intron_RP23-302F9.3_chr8_87235431_R | GTCTCGTGGGCTCGGATCTGCATTCCTGGAAGGCC | 60.1 | 0.07 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106442366_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm25672|Barhl2_chr5_106442366_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Zfp69_chr4_120935939_F | TCGTCGGCAGCGTCCCTCCTGTTCCACACACACA | 59.8 | 0.06 |
| mm4_intron_Zfp69_chr4_120935939_R | GTCTCGTGGGCTCGGTGGAGGGAACATGAAGCTGG | 59.6 | 0.06 |
| mm4_intergenic_Gm15676|Fam57b_chr7_126811254_F | TCGTCGGCAGCGTCAGACCATCGGTGCTGAGTTG | 60.0 | 0.06 |
| mm4_intergenic_Gm15676|Fam57b_chr7_126811254_R | GTCTCGTGGGCTCGGAGAAACAGCCCAGCACTAGC | 60.3 | 0.06 |
| mm4_intron_Clstn2_chr9_97664755_F | TCGTCGGCAGCGTCAAAAGCCACAAAGCAGCAGG | 59.8 | 0.06 |
| mm4_intron_Clstn2_chr9_97664755_R | GTCTCGTGGGCTCGGTCCCTGGGCAAGCATTCTAC | 59.7 | 0.06 |
| mm4_exon_Prrt2_chr7_127021022_F | TCGTCGGCAGCGTCGTCCACACCTACCAGCAGTC | 60.0 | 0.06 |
| mm4_exon_Prrt2_chr7_127021022_R | GTCTCGTGGGCTCGGCACAGACTTGTTCCCTCCCC | 59.9 | 0.06 |
| mm3_intergenic_Scarf2|Car15/Gm20518_chr16_17834164_F | TCGTCGGCAGCGTCGTTGGTTTCGTCCTCCAGGG | 60.6 | 0.06 |
| mm3_intergenic_Scarf2|Car15/Gm20518_chr16_17834164_R | GTCTCGTGGGCTCGGGCTGGAGGCACTGGAGTTTT | 60.5 | 0.06 |
| mm4_exon_Smim11_chr16_92311377_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_exon_Smim11_chr16_92311377_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Socs3_chr11_117969295_F | TCGTCGGCAGCGTCCCGGCCGGTCTTCTTGTAAT | 60.1 | 0.05 |
| mm4_intron_Socs3_chr11_117969295_R | GTCTCGTGGGCTCGGGTCTCCCTCTCGGTGAGTCT | 60.0 | 0.05 |
| mm4_intergenic_Syf2|Gm16225_chr4_135012924_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Syf2|Gm16225_chr4_135012924_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Wdr61|Crabp1_chr9_54740729_F | TCGTCGGCAGCGTCGCTGCAGGACTGAAGGGAAT | 60.0 | 0.05 |
| mm4_intergenic_Wdr61|Crabp1_chr9_54740729_R | GTCTCGTGGGCTCGGACAAGAGACTGGCTGCTGTG | 60.2 | 0.05 |
| mm4_intron_Tbc1d5_chr17_51007839_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Tbc1d5_chr17_51007839_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Col4a2_chr8_11350111_F | TCGTCGGCAGCGTCCCAAGCTGATGGCAAGGAGA | 60.0 | 0.05 |
| mm4_intron_Col4a2_chr8_11350111_R | GTCTCGTGGGCTCGGTAGCCCATCCAATCATCCGC | 59.9 | 0.05 |
| mm4_exon_Rapgef3/Gm16256_chr15_97767736_F | TCGTCGGCAGCGTCACAGGGCGTTCAAGCGAAG | 60.9 | 0.04 |
| mm4_exon_Rapgef3/Gm16256_chr15_97767736_R | GTCTCGTGGGCTCGGTACCGTAAAGCTCGTGCCTG | 60.1 | 0.04 |
| mm4_intergenic_Mir204|Trpm3_chr19_22760831_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Mir204|Trpm3_chr19_22760831_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Crry-ps|AC161214.1_chr8_74253934_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Crry-ps|AC161214.1_chr8_74253934_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Id2|Gm22527_chr12_25374330_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Id2|Gm22527_chr12_25374330_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Cacna1c|Gm25905_chr6_118802303_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Cacna1c|Gm25905_chr6_118802303_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Chrm3|Gm26861_chr13_9974434_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Chrm3|Gm26861_chr13_9974434_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_5990830_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_5990830_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Gm26815_chr8_121287455_F | TCGTCGGCAGCGTCTCACCTGTCCATCACTCTCCA | 60.2 | 0.04 |
| mm4_intron_Gm26815_chr8_121287455_R | GTCTCGTGGGCTCGGGAGACTTGGTCTTGCCTGCT | 59.9 | 0.04 |
| mm4_intron_Camkmt_chr17_85390680_F | TCGTCGGCAGCGTCACTTTGTGGACGAGCTTCCT | 59.2 | 0.03 |
| mm4_intron_Camkmt_chr17_85390680_R | GTCTCGTGGGCTCGGCCGAGGGATCAGACTCAACC | 59.5 | 0.03 |
| mm4_intergenic_Gm8770|Gm5939_chrX_78787297_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm8770|Gm5939_chrX_78787297_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_5467259_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_n-R5s30|Rbfox1_chr16_5467259_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Zfhx3|D030068K23Rik_chr8_109034502_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Zfhx3|D030068K23Rik_chr8_109034502_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23786|Rbbp7_chrX_162760182_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm23786|Rbbp7_chrX_162760182_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Fblim1_chr4_141599644_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Fblim1_chr4_141599644_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gas6|1700029H14Rik_chr8_13515139_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gas6|1700029H14Rik_chr8_13515139_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Kcnip3|Prom2_chr2_127522283_F | TCGTCGGCAGCGTCAAACCCTGGGGCTGTTGATC | 60.2 | 0.01 |
| mm4_intergenic_Kcnip3|Prom2_chr2_127522283_R | GTCTCGTGGGCTCGGCCCTTGTCGTCCTCTTTCCC | 60.0 | 0.01 |
| mm4_intergenic_4930571K23Rik|Kdm8_chr7_125409870_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_4930571K23Rik|Kdm8_chr7_125409870_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Tet3_chr6_83403136_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Tet3_chr6_83403136_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_4930506M07Rik_chr19_58986157_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_4930506M07Rik_chr19_58986157_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Rnf157_chr11_116359753_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Rnf157_chr11_116359753_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ipmk|Gm22937_chr10_71540510_F | TCGTCGGCAGCGTCTGGGCTGATAATGGCTGGAG | 59.5 | 0.01 |
| mm4_intergenic_Ipmk|Gm22937_chr10_71540510_R | GTCTCGTGGGCTCGGCCGTCAGGTTGTGTTCGTTG | 59.6 | 0.01 |
| mm4_exon_Nbea_chr3_56183416_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_exon_Nbea_chr3_56183416_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172627570_F | TCGTCGGCAGCGTCGCAGAGAAGGCGTTGGGTTA | 60.3 | 0.01 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172627570_R | GTCTCGTGGGCTCGGAGTCCTAGGACCGTGCCTAG | 60.1 | 0.01 |
| mm4_intron_Slc38a10_chr11_120145327_F | TCGTCGGCAGCGTCGCTCTGAAGACCGGGAAGAC | 60.1 | 0.01 |
| mm4_intron_Slc38a10_chr11_120145327_R | GTCTCGTGGGCTCGGTGATCCTAAACAAGGCGGCT | 59.3 | 0.01 |
| mm4_intron_Abca4_chr3_122125250_F | TCGTCGGCAGCGTCTTACATGTGGACCACCGGTG | 59.9 | 0.00 |
| mm4_intron_Abca4_chr3_122125250_R | GTCTCGTGGGCTCGGCAAGCAAGCCGGCATGAAAG | 60.7 | 0.00 |
| mm4_intergenic_Psma5|Gm12523/Sort1_chr3_108283794_F | TCGTCGGCAGCGTCAACGTGACCCAACCCTAACC | 59.8 | 0.00 |
| mm4_intergenic_Psma5|Gm12523/Sort1_chr3_108283794_R | GTCTCGTGGGCTCGGGAGGTCATTCTCCACCGTCG | 60.1 | 0.00 |
| mm4_intergenic_Neo1|Gm7589_chr9_59036785_F | TCGTCGGCAGCGTCCTAACCCGCGGGAGAGATTT | 59.5 | 0.00 |
| mm4_intergenic_Neo1|Gm7589_chr9_59036785_R | GTCTCGTGGGCTCGGTCTGCCTCAGGTCTCCGTAG | 60.3 | 0.00 |
| mm4_intron_Cntnap2_chr6_47176551_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Cntnap2_chr6_47176551_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10784|Gm17611_chr13_49957658_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm10784|Gm17611_chr13_49957658_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Ssbp4_chr8_70600820_F | TCGTCGGCAGCGTCTCTCCTGCACTGTAGTTGCAG | 59.9 | 0.00 |
| mm4_intron_Ssbp4_chr8_70600820_R | GTCTCGTGGGCTCGGGAATAGGAACAGGCAGCGGA | 59.8 | 0.00 |
| mm4_intergenic_Klhl31|Gclc_chr9_77754407_F | TCGTCGGCAGCGTCTCAGTCCTTATTCGCAGACAG | 57.4 | 0.00 |
| mm4_intergenic_Klhl31|Gclc_chr9_77754407_R | GTCTCGTGGGCTCGGCGCCGCGCTCCAGATTTTAT | 61.4 | 0.00 |
| mm4_intron_Eif3h_chr15_51793087_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Eif3h_chr15_51793087_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dtwd2_chr18_49740167_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Dtwd2_chr18_49740167_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Tcf3|Gm25044_chr10_80459252_F | TCGTCGGCAGCGTCGCCGGAAACATTGACGAACC | 60.1 | 0.00 |
| mm4_intergenic_Tcf3|Gm25044_chr10_80459252_R | GTCTCGTGGGCTCGGCAGTGTCTCCTCCAGCACTG | 60.0 | 0.00 |
| mm4_intergenic_Gm25327|Ninj2_chr6_120189716_F | TCGTCGGCAGCGTCAGGGACCCTGGATACACAGT | 59.5 | 0.00 |
| mm4_intergenic_Gm25327|Ninj2_chr6_120189716_R | GTCTCGTGGGCTCGGCACAAGCCCCAGAGACTTGT | 59.8 | 0.00 |
| mm4_intergenic_Gm12066|Gm12068_chr11_24262462_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm12066|Gm12068_chr11_24262462_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Rgma_chr7_73409352_F | TCGTCGGCAGCGTCGGAGTTCTGTGCAGCCCTG | 60.6 | 0.00 |
| mm4_exon_Rgma_chr7_73409352_R | GTCTCGTGGGCTCGGCATGGACAGCCGAGTGGTAA | 59.7 | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52314132 |
GTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCACGGTCTGCTGGAGCGGCCATGCTTGGCCCGGGACC CCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCT |
| mm4_intergenic_9230114K14Rik|Gm25601_chr5_52327975 |
TGGCAGGAAGGGAAGAGAGAGGGCTGTTTGCAGAGGGGACAGGGGGCACAGTGGGGAGGCAGAAGGGAAAGATTGCAGGT AGCTGCTCTATTCCAGACTTGTGCTGACAGAGCCCCACCATGGTGCTTCCTTGGCTAAGC |
| mm4_intergenic_1700029N11Rik|Jarid2_chr13_44500238 |
AGTGGAAGCACTGACAACCAGAACCAGGATTTCCACGGAGGAGACTGGTCTTCCTGAGACTCAGGGACAGTCTCTCAGCC GATAGACTCATTCCCCATCTTGGCTATGGTCATGGCGGTTA |
| mm4_intron_Cntn5_chr9_10786995 |
ACATGATGAGAAAAGGACTGTGCTAATACAAGCACCTGAGGATGTCTGAGGAGGGAACTGGAAACTTTAGAACGATGTAG GTGCTGGGAGCCAGACTGGGTTCATTCCA |
| mm3_intron_Tmem55a_chr4_14883738 |
ATGTAGAGCCATTTTATAAGGATGCTGGCTGTCCTCTGAGGGGACTGGTATTGATATATTTATTTAGTCTTAATTTACTA AGAGACAATATTAAACCCAATGCACTTCAGTTCT |
| mm3_exon_Pkd1_chr17_24571507 |
CTCGAGGCCTTTCACCCATTGATCTGTCCTTGCTGCCTCTGGGCCATACCCACTGATGCCTGTCTCCTCAGCTGACAGCC TCTAACCATGTGAGCAACATCACCGTGAACTACAATGTCACTGTGGAGCGGATGAA |
| mm4_intergenic_Kcnk18|Slc18a2_chr19_59259925 |
CACCACAAGGCTGCTCCTAGCAGGCTCCTGCTGACAACAGGCGAGCATTAATAAAACAAGGCCAGACCCCTCTGCTGAGA GCCTGCGACAGTAACAGGGATGTTAAGTGGCTAAGAACACAGCTCTGACCCCTC |
| mm3_exon_Lrrc24_chr15_76718365 |
CTGGACCAAGCCCACAAATGCGCCGCCACGCAATCCCCGAAGCCGGTTGCCAGTGAGCGCCAGTTCAAGCAGGCGAGGCT GTGCGCGGAAGGCACCGGACTCCAGCGCACGCAGGGTATTGTTGTG |
| mm4_intergenic_9330198N18Rik|Gm14488_chr2_30707709 |
TGCTCGCAGAAGGATGTTGATGGGAGGGAAAAAAGGAAAAAGCACCGGGCCGGGTCTTCCCACGCCCCCGTCACCCCGTC CCCTCCGCGGTCGAGCTGGGAGGTCCGCAGCCGCCTCTTGCGCAGCTACCC |
| mm4_exon_9330160F10Rik/2310047M10Rik_chr11_69059149 |
ACGTCTGATTCTTTCACCTATGGAATATTTTCTCTTGTGACAACAAACAAACAAACAACCCTCAATATTATCCTCCAATG AGAGAAATGCTCTACGCAGAGGGGACAGGAGGCCGAAGAGTGACAAAAGC |
| mm4_exon_Phyhip_chr14_70468699 |
GATGGGCCAAGAGATCTGGGATCCTGGGAACTATGTAGAGAAGAGGATTTGACACCCCCCACCCACCCACCCAAGGCCCT TCCCCTCTGCTGACAGCATTGTTGTGGTCGTGGCCTGCTGCCTTGTCTTCTGTCC |
| mm4_intron_Col2a1_chr15_98003910 |
GGGGAGAGCCGAGTTTCAAAGAAGGGCTGCCCGGCTGCACCTTACTGTGGGCCAGCCTGATAGCGGAGGGGACTGGAGAG CTGCGCTGAAGTCGCCGGGAGCCGCTTTTTCTTTGTGGCCTCAGATTTCAACCCA |
| mm4_intron_Pced1b_chr15_97379606 |
TGAAGGTGGCCATGACAGTCTTGTACCCCTCTGCCTCCAACCCCGCCCTCTCCTCAGACAGCCTTGGGCACTTGTGCACA ATCTCTGTAGGACCAGCTCCC |
| mm4_intron_Ift122_chr6_115897458 |
TGCTTGGAATACAGCAGCCAGATAAGGGTGTGCTCAGAGGGGACTGGTTCATTAGTATGCTTTTCAAATCCTCATTCAGG TTGTACCTCTCTGCTGTACGATCAGCTGATCTTGTGCAACGT |
| mm4_intron_Por_chr5_135732612 |
AAGATCAGGTGCCTGCTTCCTCCAGTCTCCCCTGCGGCCAGCCTGGCCCCAAGTCCTCTGCGTGCTTCCTCTTGACTCTT GCACTCCCGT |
| mm4_intergenic_1700116B05Rik|Gm24925_chr16_50848039 |
ACATCTAGGGGCTGGCTGTAAAGCTCTCTGCTGAGGGGACTGGGATTTCTTTCAGGATTTCCTTCCCTATGTTTTGTATG CTTTTAGGCAGACGATGTTGGGTTTGTTTCCCATGCTCTTCTTGCTGG |
| mm4_exon_Dffa_chr4_149104384 |
AGTCTCGAGGAGCTGAGGAGCAAAGGTTGGCTGCTGAGGGGACCGGGCGGGGTCGGGAAGGTGACCCCAGAGCCTAGGGA CTGCAGGGACCCACGTGGGTCCAGTGATTGA |
| mm4_intron_Hdac4_chr1_91958283 | ATCAACAGGACAAGGCCCAGTGCTCAATCAGGCTGTCCACTGGGGAGACAGGTGGGCATGCATGCTCTCCCCAGAAC |
| mm4_intergenic_Fnta|Hook3_chr8_26015616 |
CAGCAGAGGTGGTGGTGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGGCCCACTTCCGGTTCCTCGGGT CCTACCATAGAGGCGTGGGGTAGAGG |
| mm4_intergenic_Qprt|Spn_chr7_127129624 |
AGCCTAGTCATTTGCGCCATCACAGAAGGTGCTGTAGGAGTGTAGACAGTCTGTCTGAGGAGGGGGCAGGGCAAAGGGAC AACTTCACTAAGGACTGACATTTGAGTCAGAGCTTCATGGTTGAGGAGGAGTCTGGT |
| mm4_intergenic_2210404O07Rik|Nfic_chr10_81395463 |
CTTGGCCACTGAGAGCTCTGCCTGGGCTCAGGCTGTCCACAGAGGTGTCTGGTTGGTTCTCATGAAGGTCATGATTTTAG ACTCATGTCTCAAAAGACAGAGGACTCTGTTCCTCTGCAATGCTGTGGAG |
| mm4_intron_Hydin_chr8_110594529 |
TTCCCCATCTCCAATTGCCCTCGTCTGTCCCCATTCACCCACTCCTGCTCTTCCCGTCCCCTCCCCAGAGAGTCTCCCCT CTGCTTTCTCCTCATGCCCA |
| mm4_exon_Ttyh1_chr7_4133237 |
AGGTCAGCAGGGGAGAGAGGGCAGGCTCCCCAGGGAGGGGACAGGAGGCAGTACCCAGGGCCACTGGTGGGGATGGGGCA GCCTCCTGACTCGTTTCCAT |
| mm4_intron_Rab37_chr11_115154470 |
CATAACCCCTGGGCTCATCCCAGTCTCTAGGGTTCTAGGCTGACCTCGGAGGGCATGGGGTAAGGCAGATCTGAAAGACT AAGACTCCCCCACCCTAGGAACAAAAACCCAGTTCCTTCTGCCACACT |
| mm4_intron_Spef2_chr15_9688079 |
TGAAAAGGCAGGACTGAAGCATAAACAACAATTAAACACTAAACAAGAGGCTGTCCGAGGAGATGTCAGGAGTGGAGGGG TTGTAGGGAA |
| mm4_exon_Zfp191_chr18_24011970 |
CACGGCGTCCTTTTCCTGTAAACCACCAGGGTTTTAGTCCAGTCCCCTCCCCACAGAGCCTTGTCTGAGCTTCCTTCTCA AAAGGAACACGGCGTCAG |
| mm4_exon_Ipp_chr4_116507581 |
CGCAGTCACCTGTCACCAAAGACTGTCCCCGCCCCCTCCGCTGCCTGCCTGCGCGCGCAGAGCACCGGAGGGCGCCGGCG GGAAGGGACGCGGCGGGTAAGCGAGGCCTGCGTGCGGTCTGTGTTTCTCA |
| mm4_exon_Fmn2_chr1_174502396 |
CGAGAGGATGTGCTGGACTCACAGGCCCTGCCGATTGGGGAGCTGGACAGCGCTCACTCTATAGTCACCAAGACCCCGGA CCTCAGCCTCTCCGCTGAGGAGACAGGCCTATCGGATACCGAGTGTGCTGACCCTTTCGA |
| mm4_intergenic_Gm13816|Gm13791_chr2_92807509 |
TCGCTTTCACATCTGGCTCTTTTTTCCCTGTGACACAGGCTTCCCGCGGTGGGGGCCGGTGGGGGTGAGAAAAATCAATG CAAGCCCTGTCGCCATCTGGCCTGGTAACAGGACCAGAGGGGTGACTAGGGCAAGGGAAG |
| mm4_exon_Cnp_chr11_100576059 |
ACCTTACAACCCAGCTGTTGAAGATACTGAAGGATCTACTGCGAGGAAGAAAATCCACCATTCCCCTCCGGGGAAAGCAT CCACCATTCAAGGGTGCTTTAAGTTAGGCTTGGGGATTCCTCAACTGACAAGCGGTC |
| mm4_intergenic_Gm11958|Gm24803_chr11_4309191 |
ACAAGGCTCCCCACATTTCTCCCCGCCCCCTCAGCTGAGAGCCTTCAAGGACCTCAGAGTGTTCCCTCTCTTCAGTGGCC AAAGCCAATACAGTGTGAGCCTTTATATTTCCAGACAGCAGGGCTC |
| mm4_intergenic_Gm25895|Samt1_chrX_153971956 |
AGATGTGGGCAGTGGACATGTGAGAGCAGGTGACTTTAGAGGACAAAGGGAAGCTTCATGTGTATAAGTGCCTGTCCCTG CCGAGGACAGACTGGCAGTGTGCATGTGAGTATAGGTGCCTCGAGAGACAAGCAT |
| mm4_intron_Zbtb7a_chr10_81142765 |
CAAAGGGACCTGGATCACCCTGACTAGGGGATATGCCCCCTCCCTCTCTGTGTCAGGCTGTGGTGGGAGGGGACAGGCTT GTCTTGTCTGCCCACATGGTC |
| mm4_intron_RP23-302F9.3_chr8_87235431 |
CCCAGTGCAGAACTCTCCTCATCCCAGTTCCCTCCAGTGACAGCCTCACCTCACATGCTAAGGAAGGAACTGCACTAACC ACTTATCAACCCTCCCCCAGGGGCCTTCCAGGAATGCAGAT |
| mm4_intron_Zfp69_chr4_120935939 |
CCTCCTGTTCCACACACACAGTCTCCTGTTCCCCGCACACAACCTCCTGTCCCCCCCCAGCTTCCTGTTTCCCCCCACAG CCTCCTGTTCCCTCCCCCCACAGCCTCCTGTTCCCCCCCAGCTTCATGTTCCCTCCA |
| mm4_intergenic_Gm15676|Fam57b_chr7_126811254 |
AGACCATCGGTGCTGAGTTGGGTTCTGCGCTCCCCGGCCTCATCACAGGGTATGGACTTCTCTGTGTCTGCCTGGCCCCT CCTCGGTCAGACTGCTAGTGCTGGGCTGTTTCT |
| mm4_intron_Clstn2_chr9_97664755 |
AAAAGCCACAAAGCAGCAGGGAAGGGTTTGTAGTGTAAGTCTTTCCTCGGAGGTGACTGGGTTGCCACTTTTCCAAAGCA TCAGTAGAATGCTTGCCCAGGGA |
| mm4_exon_Prrt2_chr7_127021022 |
GTCCACACCTACCAGCAGTCTCAGTTGGTCCCAGTCTCATCCGCTGCCAGCCTCGGGACTGCTCCTGCGTCNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNAGACCGGGGAGGGAACAAGTCTGTG |
| mm3_intergenic_Scarf2|Car15/Gm20518_chr16_17834164 |
GTTGGTTTCGTCCTCCAGGGCAGCGGAGAACGGACCGGACACCCCCGCGGACAGCCTGGCGAACCGAGCCATGGCCCGCA AGAACCACAAGAAAACTCCAGTGCCTCCAGC |
| mm4_intron_Socs3_chr11_117969295 |
CCGGCCGGTCTTCTTGTAATGTTTAGTCACTACTCTGCACTGAAAGGCTGTGCGCGGAGGGCGAGGGAGGGGCCGCGGAG GGCGGGCTTGGAGCTGGGGCCTCCAGGACCCGCCGAGACTCACCGAGAGGGAGAC |
| mm4_intergenic_Wdr61|Crabp1_chr9_54740729 |
GCTGCAGGACTGAAGGGAATCCTGGGAGATCTTGCTATTCAGGGACACACACCCCCCAGCCCCCTCCCCGGCCAGCCCAG GAGGCTGTGAGGCACAGCAGCCAGTCTCTTGT |
| mm4_intron_Col4a2_chr8_11350111 |
CCAAGCTGATGGCAAGGAGACGTTAGTGCCCCCTCCCCTCCGCTGATAGCCACAGCCAGTCTTCTGTCTTGTTTGCAGAG CTGATCTAGTAGGCAGGGTTCGGCATCTCATATGGCGGATGATTGGATGGGCTA |
| mm4_exon_Rapgef3/Gm16256_chr15_97767736 |
ACAGGGCGTTCAAGCGAAGCTGCAGCTGGCACTGGTCCGGGCGGGAGCTGTCACACCCCCTGCGCTGCCCGCCCCTTCCC CTCCCCGGACAGGCCGAGGGGGCGGGGAGCAGGCACGAGCTTTACGGTA |
| mm4_intron_Gm26815_chr8_121287455 |
TCACCTGTCCATCACTCTCCATCCATTTTACCTGTCACCTCCACGGACAGTGTACCTAAGCAGCAGGCAAGACCAAGTCT C |
| mm4_intron_Camkmt_chr17_85390680 |
ACTTTGTGGACGAGCTTCCTTAAACTCAAAAGGGAAGGCTGGGCGGGGAGGGGGCGGGGCTTACTGACGGATCGATGGTT GAGTCTGATCCCTCGG |
| mm4_intergenic_Kcnip3|Prom2_chr2_127522283 |
AAACCCTGGGGCTGTTGATCCCGAGGCAGGAAAGCTGTCCCCAGAGGGGAGGGGACAGTTCTGCCATCTGCACAGCAGGA CTTCACTCCGGGCAAGAGACTCTCCCACAGAGGGCTCTCTGGGAAAGAGGACGACAAGGG |
| mm4_intergenic_Ipmk|Gm22937_chr10_71540510 |
TGGGCTGATAATGGCTGGAGAATCCAGAGATATGCGGTCAGAATAAGTAAGGGAAGGCTGACCAGGGAGGGGAGTGGGAC TCTAGAATAAGACTGCAACGAACACAACCTGACGG |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172627570 |
GCAGAGAAGGCGTTGGGTTAGATGAGGAGGCTGTCCAGGGATGGGATTGGGCTAATGTGAAGCTGTGGGGTCCTCAGAGG TGGAGCCGGGGTGTCAGGTCGGGCTTGGCTTCATCTCTAGGCACGGTCCTAGGACT |
| mm4_intron_Slc38a10_chr11_120145327 |
GCTCTGAAGACCGGGAAGACTGAGGGTTCAGAAGACTGTCTGGGGAGGGGAGGGGGCAGGAGATTGTCTGAGCGCCTAGA AGATTCTATGAGGGCCTATATAAGTAAGCCGCCTTGTTTAGGATCA |
| mm4_intron_Abca4_chr3_122125250 | TTACATGTGGACCACCGGTGCTATAGACTGTCCTGGGAGGGGAGGGGGGCTTTCATGCCGGCTTGCTTG |
| mm4_intergenic_Psma5|Gm12523/Sort1_chr3_108283794 |
AACGTGACCCAACCCTAACCCGAGGGTCTCCGGAAGCGCCGCCAACCTGCAGGCTGAGCGGGGAGGGGAGAGGGGTAACC CCTAGGCCAACTCCGGGAGCCCAGCCCGCAGACGCCGAGCGACGGTGGAGAATGACCTC |
| mm4_intergenic_Neo1|Gm7589_chr9_59036785 | CTAACCCGCGGGAGAGATTTCCCCTCACCTCCGCGGACCGCTTCAAAGGCTACGGAGACCTGAGGCAGA |
| mm4_intron_Ssbp4_chr8_70600820 |
TCTCCTGCACTGTAGTTGCAGTACTCTGGATAGGGTCCCCAGCCCCCAAACCTCCTTAGAAGGTCAAGAACCAGGCTTGA CAGGCTGTGGGGGGAGGGGAGAGGGTGCTTACAGGCCACTTCCGCTGCCTGTTCCTATTC |
| mm4_intergenic_Klhl31|Gclc_chr9_77754407 |
TCAGTCCTTATTCGCAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTATAAAATCTGGAGCGCGGCG |
| mm4_intergenic_Tcf3|Gm25044_chr10_80459252 |
GCCGGAAACATTGACGAACCCTCAGGTACCTTCATAAAATCGATCCGGGATGAGGCTGGGAGCGGAGCGGACGGGCAGGG CACTGGGGACAGTGCTGGAGGAGACACTG |
| mm4_intergenic_Gm25327|Ninj2_chr6_120189716 |
AGGGACCCTGGATACACAGTGTCATCACGCTTGGGCGAATATTTCCTTTTCTTGGGAGATTGGTGTGGTATGGCATGCCT TGGGTGTGGACAGAGGCTGTCCAAGGAGTGGAGAGGCATTACAAGTCTCTGGGGCTTGTG |
| mm4_exon_Rgma_chr7_73409352 |
GGAGTTCTGTGCAGCCCTGCGCACCTACGCCCTGTGCACTCGGCGGACAGCCCGCACCTGCCGGGGCGACCTGGCTTACC ACTCGGCTGTCCATG |