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Contents:In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.
In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.
If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.
| Name | Primer Sequence | Tm | CFD Score |
|---|---|---|---|
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313942_F | TCGTCGGCAGCGTCAATTTGTCCGAAGCCGTGAG | 58.8 | 1.00 |
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313942_R | GTCTCGTGGGCTCGGCTCCTCCTCCTGGCCTCC | 60.4 | 1.00 |
| mm3_exon_Mtbp_chr15_55617723_F | TCGTCGGCAGCGTCCAAGGCGAACTGAGGACTGA | 59.6 | 0.46 |
| mm3_exon_Mtbp_chr15_55617723_R | GTCTCGTGGGCTCGGCTGTGGACTCGACAGGTCAC | 60.0 | 0.46 |
| mm4_intergenic_Sox2ot|mmu-mir-6378_chr3_34781630_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Sox2ot|mmu-mir-6378_chr3_34781630_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm5507|Redrum_chr18_54278819_F | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Gm5507|Redrum_chr18_54278819_R | Primer3: not found at this Tm | N.d. | 0.42 |
| mm4_intergenic_Pdcd1|Gm23389_chr1_94592796_F | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intergenic_Pdcd1|Gm23389_chr1_94592796_R | Primer3: not found at this Tm | N.d. | 0.40 |
| mm4_intron_Camk2d_chr3_126712032_F | Primer3: not found at this Tm | N.d. | 0.38 |
| mm4_intron_Camk2d_chr3_126712032_R | Primer3: not found at this Tm | N.d. | 0.38 |
| mm3_intergenic_Gm16425|Gm15359_chr8_19565507_F | Primer3: not found at this Tm | N.d. | 0.37 |
| mm3_intergenic_Gm16425|Gm15359_chr8_19565507_R | Primer3: not found at this Tm | N.d. | 0.37 |
| mm4_intron_Sfxn5_chr6_85302358_F | TCGTCGGCAGCGTCCAAAACTCCTAGGGGTGCCC | 60.3 | 0.34 |
| mm4_intron_Sfxn5_chr6_85302358_R | GTCTCGTGGGCTCGGTCCAGGGCCTTTTACTGCAT | 59.2 | 0.34 |
| mm4_intron_Gm12876_chr4_122670279_F | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intron_Gm12876_chr4_122670279_R | Primer3: not found at this Tm | N.d. | 0.34 |
| mm4_intergenic_Gm16287|Capzb_chr4_139188275_F | TCGTCGGCAGCGTCGCTCACTGAAGGCACCATCT | 60.0 | 0.32 |
| mm4_intergenic_Gm16287|Capzb_chr4_139188275_R | GTCTCGTGGGCTCGGGGAGTCCGTACTAGGGAAAGG | 58.9 | 0.32 |
| mm4_intergenic_Gm7251|Gm8674_chr13_49815041_F | TCGTCGGCAGCGTCAGTCCAGAGTTTTAAATTGGCAG | 57.2 | 0.31 |
| mm4_intergenic_Gm7251|Gm8674_chr13_49815041_R | GTCTCGTGGGCTCGGAGGTAGAGTCTAGGGTGCTGA | 59.0 | 0.31 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50429729_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50429729_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Creb3l2|Akr1d1_chr6_37479159_F | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intergenic_Creb3l2|Akr1d1_chr6_37479159_R | Primer3: not found at this Tm | N.d. | 0.30 |
| mm4_intron_Dpys_chr15_39855322_F | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intron_Dpys_chr15_39855322_R | Primer3: not found at this Tm | N.d. | 0.28 |
| mm4_intergenic_Gm25633|Gm8907_chr9_50010117_F | TCGTCGGCAGCGTCCTTCTCCCTTCCTTGTCCGT | 59.0 | 0.28 |
| mm4_intergenic_Gm25633|Gm8907_chr9_50010117_R | GTCTCGTGGGCTCGGACCTAGGCCTTGTGTGGATG | 59.3 | 0.28 |
| mm4_intergenic_Rragc|Gm12916_chr4_124013066_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Rragc|Gm12916_chr4_124013066_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Fgf16_chrX_105765576_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intron_Fgf16_chrX_105765576_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Sh3bp1|Sh3bp1/1700027A07Rik_chr15_78910762_F | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Sh3bp1|Sh3bp1/1700027A07Rik_chr15_78910762_R | Primer3: not found at this Tm | N.d. | 0.27 |
| mm4_intergenic_Gm23857|Sorcs3_chr19_48322896_F | TCGTCGGCAGCGTCCACAAGGAAACGGACTGGGT | 60.1 | 0.26 |
| mm4_intergenic_Gm23857|Sorcs3_chr19_48322896_R | GTCTCGTGGGCTCGGGAAACAGACCTTGCGGCTTC | 59.7 | 0.26 |
| mm4_intergenic_Tmx1|Gm24474_chr12_70519972_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intergenic_Tmx1|Gm24474_chr12_70519972_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Dixdc1_chr9_50730283_F | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_intron_Dixdc1_chr9_50730283_R | Primer3: not found at this Tm | N.d. | 0.24 |
| mm4_exon_Sh3pxd2a_chr19_47269929_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_exon_Sh3pxd2a_chr19_47269929_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Nr5a2_chr1_136861467_F | TCGTCGGCAGCGTCCCAGTACTTGCCTGGGTGTT | 59.8 | 0.23 |
| mm4_intron_Nr5a2_chr1_136861467_R | GTCTCGTGGGCTCGGTCAGCTGCCATGTGTTCTGT | 59.8 | 0.23 |
| mm4_intron_Gns_chr10_121382262_F | TCGTCGGCAGCGTCGACTACAACCCCAGCCGTG | 60.3 | 0.23 |
| mm4_intron_Gns_chr10_121382262_R | GTCTCGTGGGCTCGGTAGAGAAGCAGCCGGATCCC | 61.1 | 0.23 |
| mm4_intergenic_Cldn8|Krtap24-1_chr16_88583959_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Cldn8|Krtap24-1_chr16_88583959_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intron_Kdm2b_chr5_122943489_F | TCGTCGGCAGCGTCTACCGGCTGTTCAAGGGAAG | 59.6 | 0.23 |
| mm4_intron_Kdm2b_chr5_122943489_R | GTCTCGTGGGCTCGGTTGAGACCGGGAAGCAAAGG | 60.2 | 0.23 |
| mm4_intergenic_Calm1/Gm26723|Ttc7b_chr12_100221582_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Calm1/Gm26723|Ttc7b_chr12_100221582_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Klf6|Gm26259_chr13_5947824_F | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Klf6|Gm26259_chr13_5947824_R | Primer3: not found at this Tm | N.d. | 0.23 |
| mm4_intergenic_Eya1|Msc_chr1_14609250_F | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intergenic_Eya1|Msc_chr1_14609250_R | Primer3: not found at this Tm | N.d. | 0.22 |
| mm4_intron_Als2_chr1_59198982_F | TCGTCGGCAGCGTCAGCTAAAACAAGAGCCCGGA | 59.3 | 0.22 |
| mm4_intron_Als2_chr1_59198982_R | GTCTCGTGGGCTCGGTGGCTTCTACAGAAAGACGGT | 59.0 | 0.22 |
| mm4_intergenic_Gm7722|Gm14609_chrX_47313778_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm7722|Gm14609_chrX_47313778_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm23059|Gm7745_chr12_61313073_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Gm23059|Gm7745_chr12_61313073_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Npr3|Gm24074_chr15_11957576_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_Npr3|Gm24074_chr15_11957576_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Prkaa2_chr4_105090644_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Prkaa2_chr4_105090644_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_C1qtnf1/Gm11747|Gm11747_chr11_118450946_F | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intergenic_C1qtnf1/Gm11747|Gm11747_chr11_118450946_R | Primer3: not found at this Tm | N.d. | 0.21 |
| mm4_intron_Ubac2_chr14_121988572_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Ubac2_chr14_121988572_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Itga11_chr9_62771257_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Itga11_chr9_62771257_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intron_Ip6k3_chr17_27158767_F | TCGTCGGCAGCGTCATTGACCTTCACTCCAGGCC | 59.6 | 0.20 |
| mm4_intron_Ip6k3_chr17_27158767_R | GTCTCGTGGGCTCGGACTTTTCATACCGTGGTACGC | 58.6 | 0.20 |
| mm4_intergenic_Gm12157|Ublcp1_chr11_44425670_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm12157|Ublcp1_chr11_44425670_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Lrig1|Gm7833_chr6_94815780_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Lrig1|Gm7833_chr6_94815780_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_exon_Uimc1_chr13_55075854_F | Primer3: not found at this Tm | N.d. | 0.20 |
| mm3_exon_Uimc1_chr13_55075854_R | Primer3: not found at this Tm | N.d. | 0.20 |
| mm4_intergenic_Gm22084|Gm24502_chr5_75393365_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm22084|Gm24502_chr5_75393365_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Rnf168|Ubxn7_chr16_32315894_F | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Rnf168|Ubxn7_chr16_32315894_R | Primer3: not found at this Tm | N.d. | 0.19 |
| mm4_intergenic_Gm24063|Rps15a_chr7_117894638_F | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm24063|Rps15a_chr7_117894638_R | Primer3: not found at this Tm | N.d. | 0.18 |
| mm4_intergenic_Gm22321|Gm1401_chr6_33192660_F | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Gm22321|Gm1401_chr6_33192660_R | Primer3: not found at this Tm | N.d. | 0.17 |
| mm4_intergenic_Dysf|Cyp26b1_chr6_84266434_F | TCGTCGGCAGCGTCTGTGGGAGTTACAGCTCTGT | 58.2 | 0.17 |
| mm4_intergenic_Dysf|Cyp26b1_chr6_84266434_R | GTCTCGTGGGCTCGGCGCACCCAATCTCCATACCC | 60.5 | 0.17 |
| mm4_intergenic_Tlr12|Gm12958_chr4_128628816_F | TCGTCGGCAGCGTCACCATTTCTGCTGCTGTGGT | 60.1 | 0.16 |
| mm4_intergenic_Tlr12|Gm12958_chr4_128628816_R | GTCTCGTGGGCTCGGGCCATTGGTAATTCACTGGTGG | 59.8 | 0.16 |
| mm4_intron_Cfhr1_chr1_139553059_F | Primer3: not found at this Tm | N.d. | 0.16 |
| mm4_intron_Cfhr1_chr1_139553059_R | Primer3: not found at this Tm | N.d. | 0.16 |
| mm3_intron_Nr2e1_chr10_42573571_F | TCGTCGGCAGCGTCTTCAACAGGTTGGACGCTCC | 60.5 | 0.16 |
| mm3_intron_Nr2e1_chr10_42573571_R | GTCTCGTGGGCTCGGGCCTAGGAGAAGGTGGAGGA | 60.0 | 0.16 |
| mm4_exon_Sec11c_chr18_65800594_F | TCGTCGGCAGCGTCGCAGCTGAGTGTGACAGACA | 60.2 | 0.16 |
| mm4_exon_Sec11c_chr18_65800594_R | GTCTCGTGGGCTCGGGGTCCCCAAAGATGTCCAGG | 60.0 | 0.16 |
| mm4_intron_Glis1_chr4_107611463_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Glis1_chr4_107611463_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm19898|Foxn3_chr12_99286350_F | TCGTCGGCAGCGTCACGAACTGAGAGAGCTTTCGG | 60.0 | 0.15 |
| mm4_intergenic_Gm19898|Foxn3_chr12_99286350_R | GTCTCGTGGGCTCGGGTGGATCCAAGAAGATGGGCA | 60.0 | 0.15 |
| mm4_intergenic_Rbm39|Phf20_chr2_156187783_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Rbm39|Phf20_chr2_156187783_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Unc13c_chr9_73917373_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Unc13c_chr9_73917373_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm26074|Qtrtd1_chr16_43838879_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Gm26074|Qtrtd1_chr16_43838879_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Fam174a|Gm15427_chr1_95474337_F | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intergenic_Fam174a|Gm15427_chr1_95474337_R | Primer3: not found at this Tm | N.d. | 0.15 |
| mm4_intron_Smtn_chr11_3523734_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intron_Smtn_chr11_3523734_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Kcnd1|Otud5_chrX_7840445_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Kcnd1|Otud5_chrX_7840445_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Adm|B430319F04Rik/Ampd3_chr7_110731724_F | TCGTCGGCAGCGTCGTTTGAGGTCCAGGAAGGGG | 59.9 | 0.12 |
| mm4_intergenic_Adm|B430319F04Rik/Ampd3_chr7_110731724_R | GTCTCGTGGGCTCGGAGGCTGCAGCATTAGCATCT | 59.8 | 0.12 |
| mm4_intergenic_mmu-mir-6343|Gm25974_chr1_76991503_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_mmu-mir-6343|Gm25974_chr1_76991503_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm16565/Cd247|Gm23402_chr1_165801936_F | TCGTCGGCAGCGTCCCGGGGTTGCCCTTTAGAAA | 60.2 | 0.12 |
| mm4_intergenic_Gm16565/Cd247|Gm23402_chr1_165801936_R | GTCTCGTGGGCTCGGGGGAAACAGAGGCCGTGAAA | 60.5 | 0.12 |
| mm4_intron_Dusp5_chr19_53536814_F | TCGTCGGCAGCGTCCAGGTAGATGGTCTTGGGCC | 59.8 | 0.12 |
| mm4_intron_Dusp5_chr19_53536814_R | GTCTCGTGGGCTCGGGCTGAGGTCATCACCCTCAC | 60.1 | 0.12 |
| mm4_intergenic_Gm14062|Btbd3_chr2_137903787_F | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm14062|Btbd3_chr2_137903787_R | Primer3: not found at this Tm | N.d. | 0.12 |
| mm4_intergenic_Gm6098|Zbbx_chr3_74917044_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm6098|Zbbx_chr3_74917044_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm15324|Gm15326_chr13_111864969_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Gm15324|Gm15326_chr13_111864969_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Ncoa2|Tram1_chr1_13532794_F | TCGTCGGCAGCGTCAGCAGCATCTAGACTCTTTCCC | 59.5 | 0.11 |
| mm4_intergenic_Ncoa2|Tram1_chr1_13532794_R | GTCTCGTGGGCTCGGCTAGCAAGCTGAGGTGCAGT | 60.0 | 0.11 |
| mm4_intergenic_Arhgap27|Arhgap27/Gm11647_chr11_103358797_F | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Arhgap27|Arhgap27/Gm11647_chr11_103358797_R | Primer3: not found at this Tm | N.d. | 0.11 |
| mm4_intergenic_Ocel1/Nr2f6|Nr2f6_chr8_71380071_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Ocel1/Nr2f6|Nr2f6_chr8_71380071_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gprc5b|Gpr139_chr7_119033946_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Gprc5b|Gpr139_chr7_119033946_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Zfp330|Rnf150_chr8_82850423_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Zfp330|Rnf150_chr8_82850423_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Veph1|Shox2_chr3_66314826_F | Primer3: not found at this Tm | N.d. | 0.10 |
| mm4_intergenic_Veph1|Shox2_chr3_66314826_R | Primer3: not found at this Tm | N.d. | 0.10 |
| mm3_exon_Vwa8_chr14_79198437_F | TCGTCGGCAGCGTCTTGCCCAGATCCTGACAAGC | 60.3 | 0.10 |
| mm3_exon_Vwa8_chr14_79198437_R | GTCTCGTGGGCTCGGTGCTCTGTGCTGCGACAATA | 60.0 | 0.10 |
| mm4_intron_Rgs19_chr2_181690826_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Rgs19_chr2_181690826_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Il12rb1|Arrdc2_chr8_70829675_F | TCGTCGGCAGCGTCCAGTGACCCAGCTGATGAGG | 60.1 | 0.08 |
| mm4_intergenic_Il12rb1|Arrdc2_chr8_70829675_R | GTCTCGTGGGCTCGGGGTTGAGGGACATGGTGACC | 60.3 | 0.08 |
| mm4_intergenic_Gm10258|Mecom_chr3_30391369_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm10258|Mecom_chr3_30391369_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_March3_chr18_56868085_F | TCGTCGGCAGCGTCGGACCTACTTTCACACTGCCA | 59.9 | 0.08 |
| mm4_intron_March3_chr18_56868085_R | GTCTCGTGGGCTCGGTCAAACTTTAGTACTTCTCAGGGGA | 59.1 | 0.08 |
| mm4_intergenic_Gm13214|Oprd1_chr4_132097945_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Gm13214|Oprd1_chr4_132097945_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Atp2b4|Optc_chr1_133810335_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intergenic_Atp2b4|Optc_chr1_133810335_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_4930529M08Rik_chr2_146075340_F | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_4930529M08Rik_chr2_146075340_R | Primer3: not found at this Tm | N.d. | 0.08 |
| mm4_intron_Tmem64_chr4_15277688_F | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intron_Tmem64_chr4_15277688_R | Primer3: not found at this Tm | N.d. | 0.07 |
| mm4_intergenic_Gm22825|Zfhx3_chr8_107861597_F | TCGTCGGCAGCGTCTTTGCTTAGCCTGTGGTGCT | 60.1 | 0.07 |
| mm4_intergenic_Gm22825|Zfhx3_chr8_107861597_R | GTCTCGTGGGCTCGGCTCCACCTCTGGGCATCTTT | 59.3 | 0.07 |
| mm4_intron_Col22a1_chr15_72006545_F | TCGTCGGCAGCGTCCACCCACAACTCCTCTGGTC | 59.9 | 0.06 |
| mm4_intron_Col22a1_chr15_72006545_R | GTCTCGTGGGCTCGGTGAAAGTGAGTTGCCCAGCT | 59.8 | 0.06 |
| mm4_intergenic_5S_rRNA|Auts2_chr5_131608937_F | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intergenic_5S_rRNA|Auts2_chr5_131608937_R | Primer3: not found at this Tm | N.d. | 0.06 |
| mm4_intron_Pde5a_chr3_122809084_F | TCGTCGGCAGCGTCGTGCCCCGCTTCATCTATGA | 59.8 | 0.06 |
| mm4_intron_Pde5a_chr3_122809084_R | GTCTCGTGGGCTCGGCAGAGCCACAAGGACCTCAA | 59.6 | 0.06 |
| mm4_intergenic_Irf2bp2|Tomm20_chr8_126915422_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Irf2bp2|Tomm20_chr8_126915422_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm13994|Slc24a5_chr2_124765414_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intergenic_Gm13994|Slc24a5_chr2_124765414_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_4933431K23Rik_chr8_77760124_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_4933431K23Rik_chr8_77760124_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Fam117b_chr1_59969590_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Fam117b_chr1_59969590_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Ntn4_chr10_93735074_F | Primer3: not found at this Tm | N.d. | 0.05 |
| mm3_intron_Ntn4_chr10_93735074_R | Primer3: not found at this Tm | N.d. | 0.05 |
| mm4_intron_Smarcc1_chr9_110166826_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Smarcc1_chr9_110166826_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Aqr_chr2_114146808_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Aqr_chr2_114146808_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Clasp2_chr9_113909186_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Clasp2_chr9_113909186_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172789118_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tfap2c|Gm22773_chr2_172789118_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tango6|Has3_chr8_106867522_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Tango6|Has3_chr8_106867522_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Syn2|Gm17482_chr6_115205700_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Syn2|Gm17482_chr6_115205700_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Id2|Gm22527_chr12_25586400_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Id2|Gm22527_chr12_25586400_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Ubiad1_chr4_148436641_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_exon_Ubiad1_chr4_148436641_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm3_intergenic_Gm9915|2610017I09Rik_chr1_42440461_F | TCGTCGGCAGCGTCGCCTCAGGTACGTCAAGTGT | 59.6 | 0.04 |
| mm3_intergenic_Gm9915|2610017I09Rik_chr1_42440461_R | GTCTCGTGGGCTCGGTGCTGAGTGGCTGTTACTCG | 60.0 | 0.04 |
| mm4_intergenic_Lrriq3|Gm23038_chr3_155406518_F | TCGTCGGCAGCGTCACAAGTTGACTGGTGACACAGA | 59.7 | 0.04 |
| mm4_intergenic_Lrriq3|Gm23038_chr3_155406518_R | GTCTCGTGGGCTCGGTGGCCCTCATCTTTCAGACC | 59.3 | 0.04 |
| mm4_intergenic_Gm23067|Gm26072_chr5_70318637_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Gm23067|Gm26072_chr5_70318637_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Cntnap2_chr6_46761562_F | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intron_Cntnap2_chr6_46761562_R | Primer3: not found at this Tm | N.d. | 0.04 |
| mm4_intergenic_Syne4|Sdhaf1/E130208F15Rik_chr7_30319706_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Syne4|Sdhaf1/E130208F15Rik_chr7_30319706_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm12746|Ssbp3_chr4_106862968_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Gm12746|Ssbp3_chr4_106862968_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Srrm1_chr4_135333135_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Srrm1_chr4_135333135_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_BC051070|2010016I18Rik/Dennd2d_chr3_106452521_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_BC051070|2010016I18Rik/Dennd2d_chr3_106452521_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Usp25|2810055G20Rik_chr16_77131839_F | TCGTCGGCAGCGTCGGAACGGACGGAGGAATGTT | 60.0 | 0.03 |
| mm4_intergenic_Usp25|2810055G20Rik_chr16_77131839_R | GTCTCGTGGGCTCGGTCAGGAGAGCCCCAAGAAGA | 59.8 | 0.03 |
| mm4_intergenic_Commd10|Gm25036_chr18_47094620_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Commd10|Gm25036_chr18_47094620_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Zfp952_chr17_33000586_F | TCGTCGGCAGCGTCTTCTCCCCGTCTTTCAGTGC | 59.9 | 0.03 |
| mm4_intron_Zfp952_chr17_33000586_R | GTCTCGTGGGCTCGGGTCATCGAGCTAAGGCCTCC | 59.9 | 0.03 |
| mm4_intergenic_Nol10|Odc1_chr12_17449727_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Nol10|Odc1_chr12_17449727_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Katna1_chr10_7740411_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Katna1_chr10_7740411_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Stc2|Bod1_chr11_31372351_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Stc2|Bod1_chr11_31372351_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pex6_chr17_46718347_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Pex6_chr17_46718347_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Efna2|Mum1_chr10_80210956_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Efna2|Mum1_chr10_80210956_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Taok3_chr5_117237781_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Taok3_chr5_117237781_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Erp44_chr4_48211386_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Erp44_chr4_48211386_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Dscr3_chr16_94511604_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Dscr3_chr16_94511604_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tspear|1700009J07Rik_chr10_77890692_F | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intergenic_Tspear|1700009J07Rik_chr10_77890692_R | Primer3: not found at this Tm | N.d. | 0.03 |
| mm4_intron_Megf11_chr9_64642690_F | TCGTCGGCAGCGTCAAGCTTCTGTGAGGTCTGGC | 59.9 | 0.02 |
| mm4_intron_Megf11_chr9_64642690_R | GTCTCGTGGGCTCGGAGAAAGCTCCCAGGCTCTTG | 59.6 | 0.02 |
| mm4_intron_Prph2_chr17_46919108_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Prph2_chr17_46919108_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26607|Col9a1_chr1_24175267_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm26607|Col9a1_chr1_24175267_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Magi2|Phtf2_chr5_20746343_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Magi2|Phtf2_chr5_20746343_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm11479|Smg8_chr11_87075720_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Gm11479|Smg8_chr11_87075720_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tmod2|Lysmd2_chr9_75624050_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Tmod2|Lysmd2_chr9_75624050_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Tmprss5|Gm4894_chr9_49168731_F | TCGTCGGCAGCGTCAGCCAAGGTGGGAGATTTCG | 60.0 | 0.02 |
| mm3_intergenic_Tmprss5|Gm4894_chr9_49168731_R | GTCTCGTGGGCTCGGAGTTGGGCAAGTGATCTGGG | 59.9 | 0.02 |
| mm4_intron_Dcaf6_chr1_165363243_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Dcaf6_chr1_165363243_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm12295|Gm26128_chr11_65393796_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intergenic_Gm12295|Gm26128_chr11_65393796_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Scnn1g|Scnn1b_chr7_121810819_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Scnn1g|Scnn1b_chr7_121810819_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Pitpnc1|Gm11712_chr11_107381202_F | TCGTCGGCAGCGTCGTTCAGTGTGCCAGCCAATG | 60.0 | 0.02 |
| mm4_intergenic_Pitpnc1|Gm11712_chr11_107381202_R | GTCTCGTGGGCTCGGTCCCTTTCACCTCCCTCCAT | 59.8 | 0.02 |
| mm4_intron_Eva1c_chr16_90891101_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intron_Eva1c_chr16_90891101_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Kcnn3|Adar_chr3_89710307_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Kcnn3|Adar_chr3_89710307_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Il1b_chr2_129365865_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Il1b_chr2_129365865_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Slc22a23_chr13_34328680_F | TCGTCGGCAGCGTCGCCACCAGTATTCCATGGCT | 60.1 | 0.02 |
| mm3_intron_Slc22a23_chr13_34328680_R | GTCTCGTGGGCTCGGAATTCAGACAAACCCTCTCCAT | 57.3 | 0.02 |
| mm4_intergenic_Mrps6|Kcne2_chr16_92170063_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_intergenic_Mrps6|Kcne2_chr16_92170063_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Agpat3_chr10_78307422_F | Primer3: not found at this Tm | N.d. | 0.02 |
| mm4_exon_Agpat3_chr10_78307422_R | Primer3: not found at this Tm | N.d. | 0.02 |
| mm3_intron_Cdc37l1_chr19_29015008_F | TCGTCGGCAGCGTCGTTTCCCTGTGAGATGAGCCA | 59.9 | 0.01 |
| mm3_intron_Cdc37l1_chr19_29015008_R | GTCTCGTGGGCTCGGTTCTTCTTCACCCAGGCTGG | 59.5 | 0.01 |
| mm4_intergenic_C130080G10Rik|A530058N18Rik_chr2_114065174_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_C130080G10Rik|A530058N18Rik_chr2_114065174_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Barx1|Phf2_chr13_48695597_F | TCGTCGGCAGCGTCAAAGTCAAAGCAGTGCCAGC | 59.6 | 0.01 |
| mm4_intergenic_Barx1|Phf2_chr13_48695597_R | GTCTCGTGGGCTCGGGGGGAAGCCCGGAACAAATA | 60.0 | 0.01 |
| mm4_intergenic_Kif6/Gm16554|Kif6_chr17_49898582_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Kif6/Gm16554|Kif6_chr17_49898582_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Pi4k2a_chr19_42092276_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Pi4k2a_chr19_42092276_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Ube3b|Mmab_chr5_114430908_F | TCGTCGGCAGCGTCTCTGCTCAGCCTGGACAAAG | 59.9 | 0.01 |
| mm4_intergenic_Ube3b|Mmab_chr5_114430908_R | GTCTCGTGGGCTCGGCCAAGCTGGTCCTGTCTTGA | 59.6 | 0.01 |
| mm4_intergenic_Il2|Gm12532_chr3_37138734_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Il2|Gm12532_chr3_37138734_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Gm6803|Gm2056_chr12_88021479_F | TCGTCGGCAGCGTCACACCAAACAAGCAAAGAAACA | 57.7 | 0.01 |
| mm4_intergenic_Gm6803|Gm2056_chr12_88021479_R | GTCTCGTGGGCTCGGACCAGTAGGCAGTAAGAAGTGT | 58.7 | 0.01 |
| mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52113733_F | TCGTCGGCAGCGTCGACCTGCCGGCTGATCTG | 60.2 | 0.01 |
| mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52113733_R | GTCTCGTGGGCTCGGCTCGGGAGATCCAAAAGGCT | 59.4 | 0.01 |
| mm4_intron_Fam222b_chr11_78098822_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Fam222b_chr11_78098822_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Foxf2|Gm25186_chr13_31736510_F | TCGTCGGCAGCGTCACCATAGAGACCCTTGACACC | 58.8 | 0.01 |
| mm4_intergenic_Foxf2|Gm25186_chr13_31736510_R | GTCTCGTGGGCTCGGAGAAATGCAGCAGGCTCTGA | 59.6 | 0.01 |
| mm4_intergenic_Stc2|Bod1_chr11_31622832_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Stc2|Bod1_chr11_31622832_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Itpkb|6330403A02Rik_chr1_180426202_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Itpkb|6330403A02Rik_chr1_180426202_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Kcnh1_chr1_192213181_F | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intron_Kcnh1_chr1_192213181_R | Primer3: not found at this Tm | N.d. | 0.01 |
| mm4_intergenic_Eln|Wbscr28_chr5_134789809_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Eln|Wbscr28_chr5_134789809_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cdkl5|Scml2_chrX_161098612_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Cdkl5|Scml2_chrX_161098612_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lsm4_chr8_70677526_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lsm4_chr8_70677526_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pik3cg_chr12_32177567_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Pik3cg_chr12_32177567_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25076|Gm26455_chr5_118320929_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25076|Gm26455_chr5_118320929_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4931415C17Rik|Gm15348_chr8_12694232_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_4931415C17Rik|Gm15348_chr8_12694232_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Pkmyt1_chr17_23729032_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Pkmyt1_chr17_23729032_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Adap2_chr11_80164334_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Adap2_chr11_80164334_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Igbp1b|Gm25434_chr6_138884869_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Igbp1b|Gm25434_chr6_138884869_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_mmu-mir-6404|Shank2_chr7_143962713_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_mmu-mir-6404|Shank2_chr7_143962713_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Trim2_chr3_84246416_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Trim2_chr3_84246416_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tbc1d1_chr5_64296834_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Tbc1d1_chr5_64296834_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Slc6a13_chr6_121307751_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Slc6a13_chr6_121307751_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Myt1l_chr12_29735680_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Myt1l_chr12_29735680_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15843|March4_chr1_72500729_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm15843|March4_chr1_72500729_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13828|Pop5_chr5_115200374_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm13828|Pop5_chr5_115200374_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gdf11|Cd63_chr10_128893295_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gdf11|Cd63_chr10_128893295_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Grin2b/Gm14329_chr6_135715463_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_exon_Grin2b/Gm14329_chr6_135715463_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Esrrb_chr12_86402268_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Esrrb_chr12_86402268_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Skap1_chr11_96497316_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Skap1_chr11_96497316_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lactb_chr9_66965889_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Lactb_chr9_66965889_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp541_chr7_16094327_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfp541_chr7_16094327_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Psd2_chr18_36012204_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Psd2_chr18_36012204_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hkdc1_chr10_62396830_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Hkdc1_chr10_62396830_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ranbp9|Mcur1_chr13_43507115_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Ranbp9|Mcur1_chr13_43507115_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mical3|Pex26_chr6_121097539_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Mical3|Pex26_chr6_121097539_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25830|4930555F03Rik_chr8_49020337_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm25830|4930555F03Rik_chr8_49020337_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gfod1|Sirt5_chr13_43337518_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gfod1|Sirt5_chr13_43337518_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Arl2bp_chr8_94672531_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Arl2bp_chr8_94672531_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rbm24|Cap2_chr13_46433720_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Rbm24|Cap2_chr13_46433720_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfpm2_chr15_41007377_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Zfpm2_chr15_41007377_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Olfml2b_chr1_170660454_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intron_Olfml2b_chr1_170660454_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_n-R5s82|Pknox2_chr9_37029397_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_n-R5s82|Pknox2_chr9_37029397_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Snx19|Adamts15_chr9_30527485_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Snx19|Adamts15_chr9_30527485_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23494|Gm15679_chr8_98687086_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Gm23494|Gm15679_chr8_98687086_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Degs2|Gm23872_chr12_108717273_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm4_intergenic_Degs2|Gm23872_chr12_108717273_R | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Fhit_chr14_9812165_F | Primer3: not found at this Tm | N.d. | 0.00 |
| mm3_intron_Fhit_chr14_9812165_R | Primer3: not found at this Tm | N.d. | 0.00 |
These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.
| ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313942 |
AATTTGTCCGAAGCCGTGAGCAGCCCGCTGCCTGAACCGCCAGAGAAGATGGTGCCCCACGGTTGCCTGAGCCCGCGAGC CGGCCCTCCGACTTCCCGGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAG |
| mm3_exon_Mtbp_chr15_55617723 |
CAAGGCGAACTGAGGACTGAAGTTTCTGGATTGAAGCGGAGATCTAAAGACCCCAGCTGCCTGTACCCCCAGAAAAGGTG ACCTGTCGAGTCCACAG |
| mm4_intron_Sfxn5_chr6_85302358 |
CAAAACTCCTAGGGGTGCCCAGGTCCCCCAAGGCTGAGTGGCTGAGCCTCCTCCTGCTGCCTGAGCCTCTAAAGACCTGG ACAATGCATGCTCTACTTTCTAGTGAAATGCAGTAAAAGGCCCTGGA |
| mm4_intergenic_Gm16287|Capzb_chr4_139188275 |
GCTCACTGAAGGCACCATCTTGTGAATGGAGGCGCGTGTCTTTTGCAGTCCAGGCAGCTGGACCATATCCTTTCCCTAGT ACGGACTCC |
| mm4_intergenic_Gm7251|Gm8674_chr13_49815041 |
AGTCCAGAGTTTTAAATTGGCAGTCTGTGATGGTTCAGGCAACTGGCATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNTTCAGCACCCTAGACTCTACCT |
| mm4_intergenic_Gm25633|Gm8907_chr9_50010117 |
CTTCTCCCTTCCTTGTCCGTAAATCATGCACATTGGCAGAATCCTGCTGCCTAGGCTGCCAGAGAGTCATCCACACAAGG CCTAGGT |
| mm4_intergenic_Gm23857|Sorcs3_chr19_48322896 |
CACAAGGAAACGGACTGGGTTTTTAGATTAGCCATTTCCAGGGCTTAATACTAAGGACTTGGCCTTCTAATGGCTCCACC TGCTTCTTGGACCCCCAGAGATCATTCTTTGGCTTCTCCTGAAGCCGCAAGGTCTGTTTC |
| mm4_intron_Nr5a2_chr1_136861467 |
CCAGTACTTGCCTGGGTGTTGAGCACGTTAACAGCCAACTGTCACTATGCTAGTACTGATTCCTGCTGCCTGGATGGCCT GAGAAAATGACAGAACAGAACACATGGCAGCTGA |
| mm4_intron_Gns_chr10_121382262 |
GACTACAACCCCAGCCGTGGGTGGGTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNCCTGGGGTCCCATGGGATCCGGCTGCTTCTCTA |
| mm4_intron_Kdm2b_chr5_122943489 |
TACCGGCTGTTCAAGGGAAGCTTCTACCAACCTTCCATCCCCTTCTGGGGTATCACAGGTGGATCAGGCAGCCGGCCTTG GATTTAAATGGCTGCTGAGTTCCTCTGCAGCCCCGCTTACCTTTGCTTCCCGGTCTCAA |
| mm4_intron_Als2_chr1_59198982 |
AGCTAAAACAAGAGCCCGGATGATCTGGGGGTGCAGGCAGCGGGAGGTATCAACTTACCTAAACTGAGAAGAGGCCTAAG TATCTGTGATTTGATTTCGCTCAGTTTTGAGTAAAACCGTCTTTCTGTAGAAGCCA |
| mm4_intron_Ip6k3_chr17_27158767 |
ATTGACCTTCACTCCAGGCCCTGGGCATGGCTGGCTGGCTCCACAAGCAGCAGTGCCTATCACTTCATTGGACTTAGGCT TACAGAGGCTCTCCCGGCTGCCTGGGCTGCCAGCGAGCGTACCACGGTATGAAAAGT |
| mm4_intergenic_Dysf|Cyp26b1_chr6_84266434 |
TGTGGGAGTTACAGCTCTGTAAGAAAAATAAGCAGGAAATATGAAGTAGCCTGCAGTTCTGCCTGGTGCCTGGATCGCCA GGGAAGGAGGGTGGGCCCAGGGCATGGGAAAAAAGTAGGGTATGGAGATTGGGTGCG |
| mm4_intergenic_Tlr12|Gm12958_chr4_128628816 |
ACCATTTCTGCTGCTGTGGTTAGCGACTTGCCCAGTCTCCCAGACACACGGGGCCTGCTGCCAGAAAAGCCAGATAGAGT TGTCTTTTGTTCCACCAGTGAATTACCAATGGC |
| mm3_intron_Nr2e1_chr10_42573571 |
TTCAACAGGTTGGACGCTCCCTCCTGCTTCCTGAGCAGCCAGAGAGAGGGCATTCCAGACAGGCAGCCCCCACCGCAAGG CTCACTACACAGCCACCCCAGGCTCTCCTCCACCTTCTCCTAGGC |
| mm4_exon_Sec11c_chr18_65800594 |
GCAGCTGAGTGTGACAGACAGAGCCGGCCGGCCGCCTGGGCCCCCAGAGACCCCGCCATGGTTCGTGCGGGCGCCGTGGG GACGCATCTCCCCACGTCCAGCCTGGACATCTTTGGGGACC |
| mm4_intergenic_Gm19898|Foxn3_chr12_99286350 |
ACGAACTGAGAGAGCTTTCGGGAACGATCATGTTTGATATCTGGTTGTTCGGGCAGCTGGCAAACACATTCTGCCCATCT TCTTGGATCCAC |
| mm4_intergenic_Adm|B430319F04Rik/Ampd3_chr7_110731724 |
GTTTGAGGTCCAGGAAGGGGACCTCTCTGGAGCCCAGGATTTTGTGGCCTGCTGTGGAGCTTCAGGCAGCTGGCAGCTGA ACTCTGCAGCATGGACCCCTGGGGCCCTGGCCTGAGATGCTAATGCTGCAGCCT |
| mm4_intergenic_Gm16565/Cd247|Gm23402_chr1_165801936 |
CCGGGGTTGCCCTTTAGAAAAACATCACCAAGCACATTCTGTAGTCTCAGAATGAGTTCGCTGGTGATTCAGGCAGTGGG GCTTGATCTGAGTTTCAGACATCTCTATTCATTTCACGGCCTCTGTTTCCC |
| mm4_intron_Dusp5_chr19_53536814 | CAGGTAGATGGTCTTGGGCCCTCTGTCTGGAGCTTCAGGCATCTGGGTGAGGGTGATGACCTCAGC |
| mm4_intergenic_Ncoa2|Tram1_chr1_13532794 |
AGCAGCATCTAGACTCTTTCCCCAAACTATTATGATTACTAATGAAAAGTTAGACCCTCATTTAGTTTAAATCTTTGGCG TGTCAGGCACCTGGCAGCATGCTATGACTGCACCTCAGCTTGCTAG |
| mm3_exon_Vwa8_chr14_79198437 |
TTGCCCAGATCCTGACAAGCGATCCTCAAGTAAACGCCTTTGCCATTTTTATCGGCTCTTTGGGGGATCAGGCAGCCAGG TGAGTATTGTCGCAGCACAGAGCA |
| mm4_intergenic_Il12rb1|Arrdc2_chr8_70829675 |
CAGTGACCCAGCTGATGAGGCCTGCTGCCCTGGTACTGACCCCAGCACCCCCACCCCCTGCCGCCTGCACCGCCAGGGTC ACATGACACATCTGAGTCGCAGCCTGGTCACAGGGTCGGTCACCATGTCCCTCAACC |
| mm4_intron_March3_chr18_56868085 | GGACCTACTTTCACACTGCCACATTTCCCTGGAGGATCAGGCGGCAGGCATCTCCCCTGAGAAGTACTAAAGTTTGA |
| mm4_intergenic_Gm22825|Zfhx3_chr8_107861597 |
TTTGCTTAGCCTGTGGTGCTGAGACAGCCCGGTGCCTTAACCACCTGAGACAGACAGCTCTCTGGTCTTTAAAGATGCCC AGAGGTGGAG |
| mm4_intron_Col22a1_chr15_72006545 |
CACCCACAACTCCTCTGGTCTTGGCACACTGCTGCCTGTCTCTGCCTGTTCAGGTGGCAGGTGGGGGTATCCTTGGGAGA AGACAGAACTCCACAGGGGAGCTGGGCAACTCACTTTCA |
| mm4_intron_Pde5a_chr3_122809084 |
GTGCCCCGCTTCATCTATGAAAGAAGAGAATATGAGCTTACCCTGCAGAGTTGCAGTAACAAGTTCCTGGTGCCTGAACC TCCACGGACTTCTGGTTATTGTTAGTAGTTGTAGCGATATTGAGGTCCTTGTGGCTCTG |
| mm3_intergenic_Gm9915|2610017I09Rik_chr1_42440461 |
GCCTCAGGTACGTCAAGTGTGATTTAATATCTCACACACTTCTTATGAAGACTAAAAGATTACCCGGCTGCCTCCACTGC CAGAGAGCGACCTCTGCCCCCGAGTAACAGCCACTCAGCA |
| mm4_intergenic_Lrriq3|Gm23038_chr3_155406518 |
ACAAGTTGACTGGTGACACAGATATCCAATCATGGCTATGAGCCAGGCCGCTCAGGAGGTTGAGGCAGCAGGATCATCTA GGTCTGAAAGATGAGGGCCA |
| mm4_intergenic_Usp25|2810055G20Rik_chr16_77131839 |
GGAACGGACGGAGGAATGTTCCCAGAAGTCCTCTGTTTCCTGCAGTTCATTTACAGAGTAGCCCAGCAGTGCCTGCTCCC TGGATCTCCAGAGAAGATCTTCTTGGGGCTCTCCTGA |
| mm4_intron_Zfp952_chr17_33000586 |
TTCTCCCCGTCTTTCAGTGCTCTCTGGCAGGTGAAGCAGCTGGCAGCGCACATCTGCCCTCAGCCGTTCTGTTCTTTATT TAGGGCAGGGAGGCCTTAGCTCGATGAC |
| mm4_intron_Megf11_chr9_64642690 |
AAGCTTCTGTGAGGTCTGGCCTCTCTGGCCCTTCAGGCCACTGGCCCCCAAGAATGCCATAGGCCCCTCCACTTGCCTGG CTATACTGAACCCAATGTGGGCAGGCGTGTGCCAAGAGCCTGGGAGCTTTCT |
| mm3_intergenic_Tmprss5|Gm4894_chr9_49168731 |
AGCCAAGGTGGGAGATTTCGTCTTATGTAGGGGATCAGTTTCTCTGGAGGCTCAGGCAGGAGGCCAGACCAGAAACCCAG ATCACTTGCCCAACT |
| mm4_intergenic_Pitpnc1|Gm11712_chr11_107381202 |
GTTCAGTGTGCCAGCCAATGTGTTATATATATGGCCCTGGATCTGATCTCTGGCAATTCAGGGATCGGGGATTCGGGGAT GCAGGGGAGGGGGGCGGGGGAATGGAGGGAGGTGAAAGGGA |
| mm3_intron_Slc22a23_chr13_34328680 |
GCCACCAGTATTCCATGGCTGTGTACTTTGAGAGACCTCTCTTTCTCTGCTGCCTGAAACACCAGGGAACCACCTCCCTA GGAATTAATTATGGAGAGGGTTTGTCTGAATT |
| mm3_intron_Cdc37l1_chr19_29015008 |
GTTTCCCTGTGAGATGAGCCATTTATGACATGATCTCCAGTAACCTTTGTCTTCCCACTCTCGGGAGGCTCAGGCAGCAG AGCTCAGACTGCAGGCCAGCCTGGGTGAAGAAGAA |
| mm4_intergenic_Barx1|Phf2_chr13_48695597 |
AAAGTCAAAGCAGTGCCAGCGCCTTCTCTAGAGGTTCAGGGAGAAGGAAGTATGCATTGCTCTTGAGAGCAGAGTGAGCC ATGATCTGGACTATTTGTTCCGGGCTTCCCC |
| mm4_intergenic_Ube3b|Mmab_chr5_114430908 |
TCTGCTCAGCCTGGACAAAGTGCCACCCTTCAGGAGGGTGCCACGCCCCCTGCCAGAACCCCCAAAGACAAATTTATCCC TCTTGGTGATCAAGACAGGACCAGCTTGG |
| mm4_intergenic_Gm6803|Gm2056_chr12_88021479 |
ACACCAAACAAGCAAAGAAACAAAAGGACACTTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACACTTCTTACTGCCTACTGGT |
| mm4_intergenic_Ppargc1a|mmu-mir-6417_chr5_52113733 |
GACCTGCCGGCTGATCTGGGGGCGGCCCGGCGCCCCATCGCAGCAGCGGCCGGCCGGCTGGCGGGATCTTGTGGCCCCGC TCCCGGGCGGTTGAGGCGGCAGGAGCCTTTTGGATCTCCCGAG |
| mm4_intergenic_Foxf2|Gm25186_chr13_31736510 |
ACCATAGAGACCCTTGACACCTCTAATTCTGATTCTTTGTGACCAAGTCTGAAATAGTGGTATTAAGAGTTCATAAATGA TTCTCTGGCTATGCAGGGAGCAGGTTTCAGAGCCTGCTGCATTTCT |