CRISPOR

DNA break repair outcome predictions

Guide: GATGACAGCTTAGAAAGAAG AGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`AATGACGATTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGA`	(Wild-type sequence)
271	`GCAGCAGGAGGATGACAGCTTAGAAAGAAG--GGCAATGGGGCTTCCTCCCAGAGGCGGT`	`2 bp deleted → frameshift`
233	`GCAGCAGGAGGATGACAGCTTAGAAAG-----GGCAATGGGGCTTCCTCCCAGAGGCGGT`	`5 bp deleted → frameshift`
191	`GCAGCAGGAGGATGACAGCTTAG---------GGCAATGGGGCTTCCTCCCAGAGGCGGT`	`9 bp deleted → no frameshift`
180	`GCAGCAGGAGGATGACAGCTT------------------------CCTCCCAGAGGCGGT`	`24 bp deleted → no frameshift`
179	`GCAGCAGGAGGATGACAGC----------------AATGGGGCTTCCTCCCAGAGGCGGT`	`16 bp deleted → frameshift`
166	`GCAGCAGGAGG----------------------GCAATGGGGCTTCCTCCCAGAGGCGGT`	`22 bp deleted → frameshift`
149	`GCAGCAGGAGGATGACAG--------------GGCAATGGGGCTTCCTCCCAGAGGCGGT`	`14 bp deleted → frameshift`
143	`GCAGCAGG-------------------------GCAATGGGGCTTCCTCCCAGAGGCGGT`	`25 bp deleted → frameshift`
134	`GCAGCAGGAGGATGACAGCTTAGAAAGAA--------TGGGGCTTCCTCCCAGAGGCGGT`	`8 bp deleted → frameshift`
126	`GCAGCAGGAGG-----------------------CAATGGGGCTTCCTCCCAGAGGCGGT`	`23 bp deleted → frameshift`
116	`GCAGCAGGAGGATGACA-------------------ATGGGGCTTCCTCCCAGAGGCGGT`	`19 bp deleted → frameshift`
115	`GCAGCAGGAGGATGACAGCTTAGAAA-----------TGGGGCTTCCTCCCAGAGGCGGT`	`11 bp deleted → frameshift`
114	`GCAGCAGGAGGATG-------------------------GGGCTTCCTCCCAGAGGCGGT`	`25 bp deleted → frameshift`
111	`GCAGCA------------------------------ATGGGGCTTCCTCCCAGAGGCGGT`	`30 bp deleted → no frameshift`
109	`GCAGCAGGAGGATGACAGCTTAGAA------------TGGGGCTTCCTCCCAGAGGCGGT`	`12 bp deleted → no frameshift`
109	`GCAGCAGGAGGATGACAGCTTAGAAAGA--------------------------GGCGGT`	`26 bp deleted → frameshift`
109	`GCAGCAGG--------------------------CAATGGGGCTTCCTCCCAGAGGCGGT`	`26 bp deleted → frameshift`
96	`GCA---------------------------------ATGGGGCTTCCTCCCAGAGGCGGT`	`33 bp deleted → no frameshift`
95	`GCAGCAGGAGGATGACAGCTTAGAAAGAAG-----------------------AGGCGGT`	`23 bp deleted → frameshift`
94	`GCAGCAGGAGG-----------------------------GGCTTCCTCCCAGAGGCGGT`	`29 bp deleted → frameshift`
89	`GCAGCAGGAGGATGACAGCTTAGA------------------------------GGCGGT`	`30 bp deleted → no frameshift`
89	`GCAGCAGGAGG------------------------------GCTTCCTCCCAGAGGCGGT`	`30 bp deleted → no frameshift`
87	`GCAGCAGGAGGATGACAG-----------------------------------AGGCGGT`	`35 bp deleted → frameshift`
86	`GCAGCAGGAGGATGACAGCTTAGAAAGAAG-------------------------GCGGT`	`25 bp deleted → frameshift`
84	`GCAGCAGGAGG-------------------------------CTTCCTCCCAGAGGCGGT`	`31 bp deleted → frameshift`
80	`GCAGCAGG--------------------------------GGCTTCCTCCCAGAGGCGGT`	`32 bp deleted → frameshift`
76	`GCAGCAGG---------------------------------GCTTCCTCCCAGAGGCGGT`	`33 bp deleted → no frameshift`
74	`GCAGCAGGAGGATGACAGCTTAGAAAG----------------------------GCGGT`	`28 bp deleted → frameshift`
74	`GCAGCAGGAGGATGACAGCT----------------------------CCCAGAGGCGGT`	`28 bp deleted → frameshift`
74	`GCAG----------------------------GGCAATGGGGCTTCCTCCCAGAGGCGGT`	`28 bp deleted → frameshift`
73	`GCAGCAGGAGG---------------------------------------------CGGT`	`45 bp deleted → no frameshift`
73	`GCAGCAGG----------------------------------CTTCCTCCCAGAGGCGGT`	`34 bp deleted → frameshift`
60	`GCAGCAGGAGGATGACAGCTTAG--------------------------------GCGGT`	`32 bp deleted → frameshift`
60	`GCAGCAGGAGGATGACAGC--------------------------------------GGT`	`38 bp deleted → frameshift`
60	`GCAGC--------------------------------------TTCCTCCCAGAGGCGGT`	`38 bp deleted → frameshift`
51	`GC-----------------------------------------TTCCTCCCAGAGGCGGT`	`41 bp deleted → frameshift`
50	`GCAGCAG----------------------------------------------AGGCGGT`	`46 bp deleted → frameshift`
47	`GCAGCAGGAGGATGACAG-------------------------------------GCGGT`	`37 bp deleted → frameshift`
45	`GCAGCAGG------------------------------------------------CGGT`	`48 bp deleted → no frameshift`
43	`GCAG-------------------------------------------------AGGCGGT`	`49 bp deleted → frameshift`
42	`GCAGCAGGAGGATGA---------------------------------------GGCGGT`	`39 bp deleted → no frameshift`
36	`GCAGCAGGAGGA------------------------------------------GGCGGT`	`42 bp deleted → no frameshift`
36	`GCAGCAGGAGG------------------------------------------------T`	`48 bp deleted → no frameshift`
34	`GCAGCAGGAG-------------------------------------------AGGCGGT`	`43 bp deleted → frameshift`
31	`GCAGCAGG---------------------------------------------------T`	`51 bp deleted → no frameshift`
29	`GCAGC----------------------------------------------------GGT`	`52 bp deleted → frameshift`
25	`GC-------------------------------------------------------GGT`	`55 bp deleted → frameshift`
23	`GCAG---------------------------------------------------GCGGT`	`51 bp deleted → no frameshift`

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