CRISPOR

DNA break repair outcome predictions

Guide: CCCTCCGCGGACAGCCTCTC TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`TTGGCCCGGGACCCCCAGTCCCCTCCGCGGACAGCCTCTCTGGCCAAGGGCAACCTAGTAGCTCAGACACCGAATCGGAT`	(Wild-type sequence)
382	`ACCCCCAGTCCCCTCCGCGGACAGCC---------AAGGGCAACCTAGTAGCTCAGACAC`	`9 bp deleted → no frameshift`
208	`ACCCCCAGTCCCCTCCGCGG-------------CCAAGGGCAACCTAGTAGCTCAGACAC`	`13 bp deleted → frameshift`
193	`ACCCCCAGTCCCCTCCGCGGACAGCCT-------------------AGTAGCTCAGACAC`	`19 bp deleted → frameshift`
179	`ACCCCCAGTCCCCTCCGCGGACAGC----------------AACCTAGTAGCTCAGACAC`	`16 bp deleted → frameshift`
179	`ACCCCCAGTCCCCTCCGC----------------CAAGGGCAACCTAGTAGCTCAGACAC`	`16 bp deleted → frameshift`
156	`ACCCCCAGTCCCCTCCGCGGACA-------------AGGGCAACCTAGTAGCTCAGACAC`	`13 bp deleted → frameshift`
154	`ACCCCCAGTCCCCTCCGCGG-------------------GCAACCTAGTAGCTCAGACAC`	`19 bp deleted → frameshift`
154	`ACCCCCAGTCCCCTCC-------------------AAGGGCAACCTAGTAGCTCAGACAC`	`19 bp deleted → frameshift`
150	`ACCCCCAGTCCCCTCCGCGGACAGCCTCTC------------------------AGACAC`	`24 bp deleted → no frameshift`
149	`ACCCCCAGTCCCCTCCGCGGACAG--------------GGCAACCTAGTAGCTCAGACAC`	`14 bp deleted → frameshift`
147	`ACCCCCAGTCCCCTCCGCGG--------------------CAACCTAGTAGCTCAGACAC`	`20 bp deleted → frameshift`
136	`ACCCCCAGTCCCCTCCGCGGACAGCCTC--------------------------AGACAC`	`26 bp deleted → frameshift`
133	`ACCCCCAGTCCCC----------------------AAGGGCAACCTAGTAGCTCAGACAC`	`22 bp deleted → frameshift`
129	`ACCCCCAGTCCCCTCCGCGGACAGC---------------------------TCAGACAC`	`27 bp deleted → no frameshift`
128	`ACCCCCAGTCCCCTCCGCGGACAGCCTCT-----------------AGTAGCTCAGACAC`	`17 bp deleted → frameshift`
126	`ACCCCCAGTCCCCTCCGC-----------------------AACCTAGTAGCTCAGACAC`	`23 bp deleted → frameshift`
126	`ACCCCCAGTCCC-----------------------AAGGGCAACCTAGTAGCTCAGACAC`	`23 bp deleted → frameshift`
120	`ACCCCCAGTCC------------------------AAGGGCAACCTAGTAGCTCAGACAC`	`24 bp deleted → no frameshift`
117	`ACCCCCA-----------------------------AGGGCAACCTAGTAGCTCAGACAC`	`29 bp deleted → frameshift`
116	`ACCCCCAGTCCCCTCCGCGGACA-------------------ACCTAGTAGCTCAGACAC`	`19 bp deleted → frameshift`
101	`ACCCCCAGTCCCCTCCGCGGACAG--------------------------------ACAC`	`32 bp deleted → frameshift`
101	`ACCCCCAGTCCCCT--------------------------------AGTAGCTCAGACAC`	`32 bp deleted → frameshift`
99	`ACCCCCAGTCCCCTCCGCGGAC----------------------CTAGTAGCTCAGACAC`	`22 bp deleted → frameshift`
99	`ACCCCCAGTCCCCTCCGCGGACA------------------------------------C`	`36 bp deleted → no frameshift`
94	`ACCCCCAGTCCCCTCC-----------------------------TAGTAGCTCAGACAC`	`29 bp deleted → frameshift`
90	`ACCCCCAGTCCCCTCCGCGGACAG------------------------TAGCTCAGACAC`	`24 bp deleted → no frameshift`
89	`ACCCC------------------------------AAGGGCAACCTAGTAGCTCAGACAC`	`30 bp deleted → no frameshift`
84	`ACCC-------------------------------AAGGGCAACCTAGTAGCTCAGACAC`	`31 bp deleted → frameshift`
80	`ACC--------------------------------AAGGGCAACCTAGTAGCTCAGACAC`	`32 bp deleted → frameshift`
76	`ACCCCCAGTCCC---------------------------------TAGTAGCTCAGACAC`	`33 bp deleted → no frameshift`
73	`ACCCCCAGTCCCCTCCGC----------------------------------TCAGACAC`	`34 bp deleted → frameshift`
73	`ACCCCCAGTCC----------------------------------TAGTAGCTCAGACAC`	`34 bp deleted → frameshift`
71	`ACCCCCAGTCCCCTC---------------------------------------AGACAC`	`39 bp deleted → no frameshift`
66	`ACCCCCAG------------------------------GGCAACCTAGTAGCTCAGACAC`	`30 bp deleted → no frameshift`
61	`ACC------------------------------------------TAGTAGCTCAGACAC`	`42 bp deleted → no frameshift`
56	`ACCCCC---------------------------------------TAGTAGCTCAGACAC`	`39 bp deleted → no frameshift`
54	`ACCCCCAGT----------------------------------------AGCTCAGACAC`	`40 bp deleted → frameshift`
54	`ACCCC----------------------------------------TAGTAGCTCAGACAC`	`40 bp deleted → frameshift`
52	`ACCCCCA-----------------------------------ACCTAGTAGCTCAGACAC`	`35 bp deleted → frameshift`
51	`ACCC-----------------------------------------TAGTAGCTCAGACAC`	`41 bp deleted → frameshift`
45	`ACCCCCAG------------------------------------------------ACAC`	`48 bp deleted → no frameshift`
45	`ACCCCCAGTCCCCTCCGCGGAC--------------------------------------`	`38 bp deleted → frameshift`
34	`ACCCCCAG-------------------------------------------CTCAGACAC`	`43 bp deleted → frameshift`
33	`ACCCCCAGTC--------------------------------------------AGACAC`	`44 bp deleted → frameshift`
22	`ACCCCCA----------------------------------------------------C`	`52 bp deleted → frameshift`
18	`AC--------------------------------------------------------AC`	`56 bp deleted → frameshift`
16	`AC----------------------------------------------------------`	`58 bp deleted → frameshift`

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