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The following table lists possible deletions.
Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.
| mh- Score | Sequence | Effect |
|---|---|---|
| GGCAACCGTGGGGCACCATCTTCTCTGGCGGTTCAGGCAGCGGGCTGCTCACGGCTTCGGACAAATTAGAGACCCTCTTA | (Wild-type sequence) | |
| 423 | GGGCACCATCTTCTCTGGCGGTTCAGGC-------TGCTCACGGCTTCGGACAAATTAGA | 7 bp deleted → frameshift |
| 329 | GGGCACCATCTTCTCTGGCGG------------GCTGCTCACGGCTTCGGACAAATTAGA | 12 bp deleted → no frameshift |
| 296 | GGGCACCATCTTCTCTGGCGGTTCAGG------GCTGCTCACGGCTTCGGACAAATTAGA | 6 bp deleted → no frameshift |
| 269 | GGGCACCATCTTCTCTGGC----------------TGCTCACGGCTTCGGACAAATTAGA | 16 bp deleted → frameshift |
| 256 | GGGCACCATCTTCTCTGGCGGTTCAGGC-----------------TTCGGACAAATTAGA | 17 bp deleted → frameshift |
| 242 | GGGCACCATCTTCTCTGGCGGTTCAGGC----------TCACGGCTTCGGACAAATTAGA | 10 bp deleted → frameshift |
| 208 | GGGCACCATCTTCTCTGGCGG-------------CTGCTCACGGCTTCGGACAAATTAGA | 13 bp deleted → frameshift |
| 190 | GGGCACCATCTTCTCTGGCGG-----------------------CTTCGGACAAATTAGA | 23 bp deleted → frameshift |
| 188 | GGGCACCATCTTCTCTGG---------------GCTGCTCACGGCTTCGGACAAATTAGA | 15 bp deleted → no frameshift |
| 184 | GGGCACCATCTTCTCTG--------------------CTCACGGCTTCGGACAAATTAGA | 20 bp deleted → frameshift |
| 179 | GGGCACCATCTTCTCTGGCGGTTCA----------------CGGCTTCGGACAAATTAGA | 16 bp deleted → frameshift |
| 169 | GGGC-------------------------------TGCTCACGGCTTCGGACAAATTAGA | 31 bp deleted → frameshift |
| 164 | GGGCACCATCTTCTCTGGCGGTTCAGGCA------------CGGCTTCGGACAAATTAGA | 12 bp deleted → no frameshift |
| 163 | GGGCACCATCTTCTCTGGC--------------------------TTCGGACAAATTAGA | 26 bp deleted → frameshift |
| 154 | GGGCACCATCTTCTCTGGC-------------------TCACGGCTTCGGACAAATTAGA | 19 bp deleted → frameshift |
| 143 | GGGCACCATCTTCTC-------------------------ACGGCTTCGGACAAATTAGA | 25 bp deleted → frameshift |
| 141 | GGGCACCATCTTCTCTGGCGG-----------------------------ACAAATTAGA | 29 bp deleted → frameshift |
| 126 | GGGCACCATCTTCTCTGGCGGTTCAGG-----------------------ACAAATTAGA | 23 bp deleted → frameshift |
| 120 | GGGCACCATCTTCTCTGGCGGTTC------------------------GGACAAATTAGA | 24 bp deleted → no frameshift |
| 104 | GGGCACCATCTTC-----------------------------------GGACAAATTAGA | 35 bp deleted → frameshift |
| 99 | GGGCACCATCTTCT----------------------GCTCACGGCTTCGGACAAATTAGA | 22 bp deleted → frameshift |
| 95 | GGGCACCATCTTCTCT-----------------------CACGGCTTCGGACAAATTAGA | 23 bp deleted → frameshift |
| 90 | GGGCACCATCTTCTCTGGCGGTTCAGGCA------------------------AATTAGA | 24 bp deleted → no frameshift |
| 89 | GGG------------------------------GCTGCTCACGGCTTCGGACAAATTAGA | 30 bp deleted → no frameshift |
| 86 | GGGCACCATCT-------------------------GCTCACGGCTTCGGACAAATTAGA | 25 bp deleted → frameshift |
| 82 | GGGCAC------------------------------------GGCTTCGGACAAATTAGA | 36 bp deleted → no frameshift |
| 80 | GGGCACCATCTTCTCTGG--------------------------------ACAAATTAGA | 32 bp deleted → frameshift |
| 80 | GG--------------------------------CTGCTCACGGCTTCGGACAAATTAGA | 32 bp deleted → frameshift |
| 77 | GGGCACCATCTTC---------------------------ACGGCTTCGGACAAATTAGA | 27 bp deleted → no frameshift |
| 77 | GGGC-----------------------------------------TTCGGACAAATTAGA | 41 bp deleted → frameshift |
| 74 | GGGCACCATCTTCTCTGGCGGTTCA----------------------------AATTAGA | 28 bp deleted → frameshift |
| 74 | GGGCACCATCT----------------------------CACGGCTTCGGACAAATTAGA | 28 bp deleted → frameshift |
| 73 | GGGC----------------------------------TCACGGCTTCGGACAAATTAGA | 34 bp deleted → frameshift |
| 70 | GGGCACCATCTTCTCTGGCGGTTCAGGCAG-----------------------------A | 29 bp deleted → frameshift |
| 66 | GGGCACCATCTTCTCT------------------------------TCGGACAAATTAGA | 30 bp deleted → no frameshift |
| 66 | GGGCACCATC------------------------------ACGGCTTCGGACAAATTAGA | 30 bp deleted → no frameshift |
| 60 | GGGCACCATCTTCT--------------------------------TCGGACAAATTAGA | 32 bp deleted → frameshift |
| 57 | GGGCACCATCTTCTCTGGCGGTTCAG---------------------------------A | 33 bp deleted → no frameshift |
| 57 | GGGCACCATCTTCTC---------------------------------GGACAAATTAGA | 33 bp deleted → no frameshift |
| 57 | GGGCACCA---------------------------------CGGCTTCGGACAAATTAGA | 33 bp deleted → no frameshift |
| 48 | GG------------------------------------------CTTCGGACAAATTAGA | 42 bp deleted → no frameshift |
| 45 | GGGCACCATC--------------------------------------GGACAAATTAGA | 38 bp deleted → frameshift |
| 38 | GGG-----------------------------------------------ACAAATTAGA | 47 bp deleted → frameshift |
| 36 | GGGCACCATCTTCTCTGGCGGTT----------------------------------AGA | 34 bp deleted → frameshift |
| 36 | GG------------------------------------------------ACAAATTAGA | 48 bp deleted → no frameshift |
| 31 | GGGCACCA---------------------------------------------AATTAGA | 45 bp deleted → no frameshift |
| 30 | GGGCAC----------------------------------------------AAATTAGA | 46 bp deleted → frameshift |
| 27 | GGGCA------------------------------------------------AATTAGA | 48 bp deleted → no frameshift |
| 21 | GGGCACCATCTT---------------------------------------------AGA | 45 bp deleted → no frameshift |
| 19 | GGGCACCAT-----------------------------------------------TAGA | 47 bp deleted → frameshift |