CRISPOR

DNA break repair outcome predictions

Guide: CAGGAGGAGGAGCCGGTCGA TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`GGGAGCGTGGCGGGGGAGGCCAGGAGGAGGAGCCGGTCGATGGACTAGCAGGCAGTGCTGCAGGGCTGGGCGCCGAGCCA`	(Wild-type sequence)
282	`CGGGGGAGGCCAGGAGGAGGAGCCGG-------ACTAGCAGGCAGTGCTGCAGGGCTGGG`	`7 bp deleted → frameshift`
261	`CGGGGGAGGCCAGGAGGAGGA-------------CTAGCAGGCAGTGCTGCAGGGCTGGG`	`13 bp deleted → frameshift`
245	`CGGGGGAGGCCAGGAGGAGGAGCCGGTCGA----CTAGCAGGCAGTGCTGCAGGGCTGGG`	`4 bp deleted → frameshift`
224	`CGGGGGAGGCCAGGAGGAGGAGC----------------AGGCAGTGCTGCAGGGCTGGG`	`16 bp deleted → frameshift`
224	`CGGGGGAGGCCAGGAGGA----------------CTAGCAGGCAGTGCTGCAGGGCTGGG`	`16 bp deleted → frameshift`
193	`CGGGGGAGGCCAGGA-------------------CTAGCAGGCAGTGCTGCAGGGCTGGG`	`19 bp deleted → frameshift`
179	`CGGGGGAGGCCAGGAGGAGGAGCCGG----------------CAGTGCTGCAGGGCTGGG`	`16 bp deleted → frameshift`
172	`CGGGGGAGGCCAGG----------------------------CAGTGCTGCAGGGCTGGG`	`28 bp deleted → frameshift`
166	`CGGGGGAGGCCAGGAGGAGG----------------------CAGTGCTGCAGGGCTGGG`	`22 bp deleted → frameshift`
147	`CGGGGGAGGCCAGGAGGAGGAGC--------------------AGTGCTGCAGGGCTGGG`	`20 bp deleted → frameshift`
143	`CGGGGGAGGCCAGGAGG-------------------------CAGTGCTGCAGGGCTGGG`	`25 bp deleted → frameshift`
134	`CGGGGGAGGC---------------------------------AGTGCTGCAGGGCTGGG`	`33 bp deleted → no frameshift`
129	`CGGGGGA---------------------------CTAGCAGGCAGTGCTGCAGGGCTGGG`	`27 bp deleted → no frameshift`
120	`CGGGGGAGG------------------------ACTAGCAGGCAGTGCTGCAGGGCTGGG`	`24 bp deleted → no frameshift`
116	`CGGGGGAGGCCAGGAGGAGGAGCCGGT-------------------GCTGCAGGGCTGGG`	`19 bp deleted → frameshift`
116	`CGGGGGAGGCCAGGAGGAGGAG-------------------GCAGTGCTGCAGGGCTGGG`	`19 bp deleted → frameshift`
116	`CGGGGGAGGCCAGGAGGAG-------------------CAGGCAGTGCTGCAGGGCTGGG`	`19 bp deleted → frameshift`
114	`CGGGGGAGGCCAGGAGGAGGAGC-------------------------TGCAGGGCTGGG`	`25 bp deleted → frameshift`
101	`CGGGGGAGGCCAG--------------------------------TGCTGCAGGGCTGGG`	`32 bp deleted → frameshift`
99	`CGGGGGAGGCCAGGAG----------------------CAGGCAGTGCTGCAGGGCTGGG`	`22 bp deleted → frameshift`
98	`CGGGGGAGGCCAGGAGGAGGAGCCGG----------------------------GCTGGG`	`28 bp deleted → frameshift`
98	`CGGGGGAGGCCAGGAGGAGGAGC----------------------------AGGGCTGGG`	`28 bp deleted → frameshift`
98	`CGGGG----------------------------ACTAGCAGGCAGTGCTGCAGGGCTGGG`	`28 bp deleted → frameshift`
95	`CGGGGGAGGCCAGGAGGAGGAG-----------------------TGCTGCAGGGCTGGG`	`23 bp deleted → frameshift`
94	`CGGGGGAGGCCAGG----------------------------------------GCTGGG`	`40 bp deleted → frameshift`
94	`CGGGGGAGGCCAGGAGGAGGAGCCGG-----------------------------CTGGG`	`29 bp deleted → frameshift`
94	`CGGGGGAGGC-----------------------------AGGCAGTGCTGCAGGGCTGGG`	`29 bp deleted → frameshift`
94	`CGGG-----------------------------ACTAGCAGGCAGTGCTGCAGGGCTGGG`	`29 bp deleted → frameshift`
91	`CGGGGGAGGCCAGGAGGAGG----------------------------------GCTGGG`	`34 bp deleted → frameshift`
89	`CGG------------------------------ACTAGCAGGCAGTGCTGCAGGGCTGGG`	`30 bp deleted → no frameshift`
86	`CGGGGGAGGCCAG-------------------------CAGGCAGTGCTGCAGGGCTGGG`	`25 bp deleted → frameshift`
81	`CGGGGGAGGCCAGGAGGAG--------------------------TGCTGCAGGGCTGGG`	`26 bp deleted → frameshift`
78	`CGGGGGAGGCCAGGAGG-------------------------------------GCTGGG`	`37 bp deleted → frameshift`
76	`CGGGGGAGGCCAGGAGGAGGAGCCGG---------------------------------G`	`33 bp deleted → no frameshift`
76	`CGGGGGAGGCCAGGAGGAGGAGC---------------------------------TGGG`	`33 bp deleted → no frameshift`
73	`CGGGGGAGGCCAGGAGGAGGAGCCGG----------------------------------`	`34 bp deleted → frameshift`
70	`CGGGGGAGGCCAGGAG-----------------------------TGCTGCAGGGCTGGG`	`29 bp deleted → frameshift`
69	`CGGGGGAGGCCAGGAGGAGG-----------------------------------CTGGG`	`35 bp deleted → frameshift`
66	`CGGGGGAG------------------------------CAGGCAGTGCTGCAGGGCTGGG`	`30 bp deleted → no frameshift`
66	`CGGGGG------------------------------------CAGTGCTGCAGGGCTGGG`	`36 bp deleted → no frameshift`
63	`CGGGGGAGGCCAGGAGGAGGAG-------------------------------GGCTGGG`	`31 bp deleted → frameshift`
62	`CGGGG-------------------------------------CAGTGCTGCAGGGCTGGG`	`37 bp deleted → frameshift`
60	`CGGGGGAGGCCAGGAGG--------------------------------------CTGGG`	`38 bp deleted → frameshift`
60	`CGGGGGAGGC--------------------------------------TGCAGGGCTGGG`	`38 bp deleted → frameshift`
60	`CGGGGGAGGC----------------------------------------------TGGG`	`46 bp deleted → frameshift`
60	`CGGG--------------------------------------CAGTGCTGCAGGGCTGGG`	`38 bp deleted → frameshift`
56	`CGGGGGAGGCCAGGAGGAGG---------------------------------------G`	`39 bp deleted → no frameshift`
56	`CGG---------------------------------------CAGTGCTGCAGGGCTGGG`	`39 bp deleted → no frameshift`
54	`CGGGGGAGGCCAGGAGGAGG----------------------------------------`	`40 bp deleted → frameshift`
52	`CGGGGGAGG---------------------------------------------GCTGGG`	`45 bp deleted → no frameshift`
51	`CGGGGGAGGCCAGG-----------------------------------------CTGGG`	`41 bp deleted → frameshift`
51	`CGGGGGAGGC-----------------------------------------AGGGCTGGG`	`41 bp deleted → frameshift`
51	`CGGGGG-------------------------------------------------CTGGG`	`49 bp deleted → frameshift`
49	`CGGGG--------------------------------------------------CTGGG`	`50 bp deleted → frameshift`
48	`CGGGGGAGGCCAGGAGG------------------------------------------G`	`42 bp deleted → no frameshift`
47	`CGGGGGAG-------------------------------------TGCTGCAGGGCTGGG`	`37 bp deleted → frameshift`
46	`CGGG---------------------------------------------------CTGGG`	`51 bp deleted → no frameshift`
46	`CGGGGGAGGCCAGGAGG-------------------------------------------`	`43 bp deleted → frameshift`
42	`CGGGGGAGGCCAGG---------------------------------------------G`	`45 bp deleted → no frameshift`
40	`CGGGGG------------------------------------------------------`	`54 bp deleted → no frameshift`
40	`CGGGGGAGGCCAGG----------------------------------------------`	`46 bp deleted → frameshift`
38	`CGGGG-------------------------------------------------------`	`55 bp deleted → frameshift`
36	`CGGG--------------------------------------------------------`	`56 bp deleted → frameshift`
36	`CGGGGG------------------------------------------------GCTGGG`	`48 bp deleted → no frameshift`
32	`CGGGGGAGG--------------------------------------------------G`	`50 bp deleted → frameshift`
31	`CGGGGGAGG---------------------------------------------------`	`51 bp deleted → no frameshift`
29	`CGG----------------------------------------------------CTGGG`	`52 bp deleted → frameshift`
28	`CGGGGG-----------------------------------------------------G`	`53 bp deleted → frameshift`
23	`CGG---------------------------------------------------------`	`57 bp deleted → no frameshift`

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