CRISPOR

DNA break repair outcome predictions

Guide: CTGAGCTACTAGGTTGCCCT TGG

The following table lists possible deletions. Each sequence below represents the context around the guide's target, with deleted nucleotides shown as "-".
Sequences are ranked by micro-homology score. This score is correlated to the likelihood of finding a particular deletion, as predicted by the method of Bae et al. 2014.

mh- Score	Sequence	Effect
	`ATAGAAATCCGATTCGGTGTCTGAGCTACTAGGTTGCCCTTGGCCAGAGAGGCTGTCCGCGGAGGGGACTGGGGGTCCCG`	(Wild-type sequence)
423	`GATTCGGTGTCTGAGCTACTAGGTTGCC-------AGAGAGGCTGTCCGCGGAGGGGACT`	`7 bp deleted → frameshift`
296	`GATTCGGTGTCTGAGCTACTAGGTTGCCC------AGAGAGGCTGTCCGCGGAGGGGACT`	`6 bp deleted → no frameshift`
242	`GATTCGGTGTCTGAGCTACTAGG----------CCAGAGAGGCTGTCCGCGGAGGGGACT`	`10 bp deleted → frameshift`
222	`GATTCGGTGTCTGAGCTACTAGGTTG------GCCAGAGAGGCTGTCCGCGGAGGGGACT`	`6 bp deleted → no frameshift`
193	`GATTCGGTGTCTGAGCTACTAGG-------------------CTGTCCGCGGAGGGGACT`	`19 bp deleted → frameshift`
179	`GATTCGGTGTCTGAGCTACTAGGTTGC----------------TGTCCGCGGAGGGGACT`	`16 bp deleted → frameshift`
162	`GATTCGGTGTCTGAGC------------------CAGAGAGGCTGTCCGCGGAGGGGACT`	`18 bp deleted → no frameshift`
154	`GATTCGGTGTCTGAGCTACTAGGTTGCCC-------------------GCGGAGGGGACT`	`19 bp deleted → frameshift`
150	`GATTCGGTGTCTGAG------------------------AGGCTGTCCGCGGAGGGGACT`	`24 bp deleted → no frameshift`
149	`GATTCGGTGTCTGAGCTACTAGGTTGCCCT--------------GTCCGCGGAGGGGACT`	`14 bp deleted → frameshift`
147	`GATTCGGTGTCTGAGCTACTAGGTTGCC--------------------GCGGAGGGGACT`	`20 bp deleted → frameshift`
141	`GATTCGGTGTCTGAGCTACTAG---------------AGAGGCTGTCCGCGGAGGGGACT`	`15 bp deleted → no frameshift`
136	`GATTCGGTGTCTGAG--------------------------GCTGTCCGCGGAGGGGACT`	`26 bp deleted → frameshift`
129	`GATTCGGTGTCTGAGCT---------------------------GTCCGCGGAGGGGACT`	`27 bp deleted → no frameshift`
128	`GATTCGGTGTCTGAGCTACTAG-----------------AGGCTGTCCGCGGAGGGGACT`	`17 bp deleted → frameshift`
126	`GATTCGGTGTCTGAGCTACTAGGTTGC-----------------------GGAGGGGACT`	`23 bp deleted → frameshift`
116	`GATTCGGTGTCTGAGCTACTAGGTTG-------------------TCCGCGGAGGGGACT`	`19 bp deleted → frameshift`
116	`GATTCGGTGTCTG-------------------GCCAGAGAGGCTGTCCGCGGAGGGGACT`	`19 bp deleted → frameshift`
109	`GATTCGG--------------------------CCAGAGAGGCTGTCCGCGGAGGGGACT`	`26 bp deleted → frameshift`
101	`GATTCGGTGTCTGAGCTACTAGG--------------------------------GGACT`	`32 bp deleted → frameshift`
101	`GATTCGGTGTCTG--------------------------------TCCGCGGAGGGGACT`	`32 bp deleted → frameshift`
99	`GATTCGGTGTCTGAGCTACTAGGT----------------------CCGCGGAGGGGACT`	`22 bp deleted → frameshift`
99	`GATTCGGTGTCTGAG----------------------AGAGGCTGTCCGCGGAGGGGACT`	`22 bp deleted → frameshift`
99	`GATTCGGTGTC------------------------------------CGCGGAGGGGACT`	`36 bp deleted → no frameshift`
95	`GATTCGGTG-----------------------GCCAGAGAGGCTGTCCGCGGAGGGGACT`	`23 bp deleted → frameshift`
94	`GATTCGGTGTCTGAGCTACTAGG-----------------------------AGGGGACT`	`29 bp deleted → frameshift`
90	`GATTCGGTGTCTGAGCTACT------------------------GTCCGCGGAGGGGACT`	`24 bp deleted → no frameshift`
76	`GATTCGGTGTCTGAGCTACTAGG---------------------------------GACT`	`33 bp deleted → no frameshift`
73	`GATTCGGTGTCTGAGCTACTAGG----------------------------------ACT`	`34 bp deleted → frameshift`
73	`GATTCGGTGTCTGAGC----------------------------------GGAGGGGACT`	`34 bp deleted → frameshift`
71	`GATTCGGTGTCTGAG---------------------------------------GGGACT`	`39 bp deleted → no frameshift`
69	`GATTCGG-----------------------------------CTGTCCGCGGAGGGGACT`	`35 bp deleted → frameshift`
66	`GATTCGGTGTCTGAGCTACTAGGTTGCCCT------------------------------`	`30 bp deleted → no frameshift`
63	`GATTCGG---------------------------------------------AGGGGACT`	`45 bp deleted → no frameshift`
54	`GATTCGGTGTCTGAGCTACT----------------------------------------`	`40 bp deleted → frameshift`
49	`GA------------------------------------GAGGCTGTCCGCGGAGGGGACT`	`36 bp deleted → no frameshift`
46	`GATTCG-------------------------------------------CGGAGGGGACT`	`43 bp deleted → frameshift`
45	`GATTCGGT--------------------------------------CCGCGGAGGGGACT`	`38 bp deleted → frameshift`
45	`GA--------------------------------------GGCTGTCCGCGGAGGGGACT`	`38 bp deleted → frameshift`
36	`GATTC------------------------------------------CGCGGAGGGGACT`	`42 bp deleted → no frameshift`
36	`GATTCGG------------------------------------------------GGACT`	`48 bp deleted → no frameshift`
34	`GATTCGGTGTCTGAGCT-------------------------------------------`	`43 bp deleted → frameshift`
34	`GATTCGG-------------------------------------------------GACT`	`49 bp deleted → frameshift`
33	`GATTCGGTGTCTGA--------------------------------------------CT`	`44 bp deleted → frameshift`
32	`GATTCGG--------------------------------------------------ACT`	`50 bp deleted → frameshift`
27	`GATTCGGTGTCT------------------------------------------------`	`48 bp deleted → no frameshift`
23	`GA---------------------------------------------------GGGGACT`	`51 bp deleted → no frameshift`
18	`GA--------------------------------------------------------CT`	`56 bp deleted → frameshift`

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