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Contents:

PCR primers for off-targets of ATGGGGCTTCCTCCCAGAGG CGG

In the table below, Illumina Nextera Handle sequences have been added and highlighted in bold. Primers for the on-target have been added for convenience. The table below is sorted by the CFD off-target score. Sites with very low CFD scores < 0.02 are unlikely to be cleaved, see our study Haeussler et al. 2016, Figure 2.

In the protocol by Matthew Canver, Harvard, two PCRs are run: one PCR to amplify the potential off-target, then a second PCR to extend the handles with Illumina barcodes. Please click here to download the protocol. Alternatively, you can have a look at Fu et al, 2014.

If a primer was not found, the reason is usually that the region around the off-target is too repetitive. To avoid unspecific primers, all repeats are masked for the primer design (not for off-target search). If you think that we should change the parameters here or should use different primer3 settings, please let us know.

Maximum amplicon length:     Primer Tm:

NamePrimer Sequence Tm CFD Score
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313620_F TCGTCGGCAGCGTCTTGCAGCAGGAGGATGACAG 60.0 1.00
ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313620_R GTCTCGTGGGCTCGGAGATGGCAGTGGGGAGCTA 59.9 1.00
mm4_intron_Wdfy3_chr5_102055251_F Primer3: not found at this Tm N.d. 0.78
mm4_intron_Wdfy3_chr5_102055251_R Primer3: not found at this Tm N.d. 0.78
mm4_intron_Atp2b4_chr1_133771957_F TCGTCGGCAGCGTCATCCTGTCCCGGTTAGCTCA 60.3 0.76
mm4_intron_Atp2b4_chr1_133771957_R GTCTCGTGGGCTCGGACACAGGCATCACGTCACAT 59.9 0.76
mm4_intergenic_Olfr695|Olfr697_chr7_106731243_F Primer3: not found at this Tm N.d. 0.67
mm4_intergenic_Olfr695|Olfr697_chr7_106731243_R Primer3: not found at this Tm N.d. 0.67
mm4_intergenic_Terf2|C630050I24Rik_chr8_107118266_F TCGTCGGCAGCGTCAGTGACTCTGCAAAGGAGCC 59.9 0.64
mm4_intergenic_Terf2|C630050I24Rik_chr8_107118266_R GTCTCGTGGGCTCGGGGTGGGTGTACACGTATCCC 59.8 0.64
mm2_exon_Sh2b3_chr5_121836071_F TCGTCGGCAGCGTCTATTCACCCCTCCTGGCTCA 59.9 0.63
mm2_exon_Sh2b3_chr5_121836071_R GTCTCGTGGGCTCGGTCTGAACCTTGTTCCCCAGG 59.2 0.63
mm3_intron_Elovl1_chr4_118429291_F TCGTCGGCAGCGTCCCAACTTTGAGGAGGAGGGG 59.6 0.59
mm3_intron_Elovl1_chr4_118429291_R GTCTCGTGGGCTCGGGCATCACCCTTCCCTACCAG 59.8 0.59
mm3_intergenic_5031439G07Rik|Upk3a_chr15_84996341_F TCGTCGGCAGCGTCTCACAATCAACAGGCACCCT 59.5 0.58
mm3_intergenic_5031439G07Rik|Upk3a_chr15_84996341_R GTCTCGTGGGCTCGGTTGTCCAGGCAGACCTCCT 60.1 0.58
mm4_intron_Nfic_chr10_81424584_F TCGTCGGCAGCGTCCTGCCTTGGGACACTTCTCA 59.6 0.56
mm4_intron_Nfic_chr10_81424584_R GTCTCGTGGGCTCGGCTCCTCATAGCTTTGCGGCT 60.1 0.56
mm4_intergenic_Mapk8|Ptpn20_chr14_33495309_F Primer3: not found at this Tm N.d. 0.56
mm4_intergenic_Mapk8|Ptpn20_chr14_33495309_R Primer3: not found at this Tm N.d. 0.56
mm4_intron_Hydin_chr8_110361251_F TCGTCGGCAGCGTCGACAAATGTCTGGGCGGAGA 60.0 0.53
mm4_intron_Hydin_chr8_110361251_R GTCTCGTGGGCTCGGTTTTGGGAGGGGAGGGGTG 61.1 0.53
mm2_intergenic_Fnip2|Gm3513_chr3_79530536_F TCGTCGGCAGCGTCCCCCATAGCTCAGCAGTAGC 59.9 0.53
mm2_intergenic_Fnip2|Gm3513_chr3_79530536_R GTCTCGTGGGCTCGGACGTGTGCTCTTACCTGCTC 60.0 0.53
mm4_intergenic_Gm23735|Fndc3a_chr14_72465504_F TCGTCGGCAGCGTCTCTGCTTGGACTGTGTAGGT 58.2 0.52
mm4_intergenic_Gm23735|Fndc3a_chr14_72465504_R GTCTCGTGGGCTCGGGTCACTGGAAGACGTGGGTT 59.8 0.52
mm3_intron_Hs6st3_chr14_119454579_F Primer3: not found at this Tm N.d. 0.52
mm3_intron_Hs6st3_chr14_119454579_R Primer3: not found at this Tm N.d. 0.52
mm4_intergenic_Ankfn1|Pctp_chr11_89676251_F Primer3: not found at this Tm N.d. 0.49
mm4_intergenic_Ankfn1|Pctp_chr11_89676251_R Primer3: not found at this Tm N.d. 0.49
mm3_intergenic_Als2cr12|Cflar_chr1_58707068_F Primer3: not found at this Tm N.d. 0.49
mm3_intergenic_Als2cr12|Cflar_chr1_58707068_R Primer3: not found at this Tm N.d. 0.49
mm4_intergenic_AC125320.1|Cdh18_chr15_22416128_F Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_AC125320.1|Cdh18_chr15_22416128_R Primer3: not found at this Tm N.d. 0.46
mm4_intergenic_Gm23162|Runx1t1_chr4_13737609_F Primer3: not found at this Tm N.d. 0.45
mm4_intergenic_Gm23162|Runx1t1_chr4_13737609_R Primer3: not found at this Tm N.d. 0.45
mm4_exon_Utp18_chr11_93862003_F TCGTCGGCAGCGTCCCCTGCCCTTTTCATTCCCT 59.9 0.44
mm4_exon_Utp18_chr11_93862003_R GTCTCGTGGGCTCGGTCTCCTGCAGGTTCATCTTCC 59.4 0.44
mm4_intergenic_Mirlet7b|Ppara_chr15_85724462_F TCGTCGGCAGCGTCGTCTGTCCTAGCTGGCCAAC 60.3 0.43
mm4_intergenic_Mirlet7b|Ppara_chr15_85724462_R GTCTCGTGGGCTCGGGCTGAGAGACATGAAGGCCA 59.7 0.43
mm4_intron_Fhit_chr14_9841146_F TCGTCGGCAGCGTCCTCAGCATCGACAAATGGCA 58.9 0.43
mm4_intron_Fhit_chr14_9841146_R GTCTCGTGGGCTCGGTCAGGGTGAAGTATGGACAGC 59.4 0.43
mm3_intron_Zfp800_chr6_28270443_F Primer3: not found at this Tm N.d. 0.42
mm3_intron_Zfp800_chr6_28270443_R Primer3: not found at this Tm N.d. 0.42
mm4_intron_Tmc1_chr19_20894640_F TCGTCGGCAGCGTCGAACTGTCCAGAGCAGCTGT 59.9 0.42
mm4_intron_Tmc1_chr19_20894640_R GTCTCGTGGGCTCGGTCTCTGTAAATGGCAAGTGACCA 59.9 0.42
mm3_intron_Farp2_chr1_93614552_F Primer3: not found at this Tm N.d. 0.40
mm3_intron_Farp2_chr1_93614552_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm10220|Gm7361_chr5_26168278_F Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Gm10220|Gm7361_chr5_26168278_R Primer3: not found at this Tm N.d. 0.40
mm4_intergenic_Tbr1|Gm13555_chr2_61814573_F TCGTCGGCAGCGTCCTTGGAGGTGACTGGCGAAT 60.0 0.40
mm4_intergenic_Tbr1|Gm13555_chr2_61814573_R GTCTCGTGGGCTCGGGTCTTTCTCCACCTCACCCG 60.0 0.40
mm4_intron_Setbp1_chr18_78770428_F Primer3: not found at this Tm N.d. 0.39
mm4_intron_Setbp1_chr18_78770428_R Primer3: not found at this Tm N.d. 0.39
mm4_intergenic_Faim2|Gm5808_chr15_99554438_F TCGTCGGCAGCGTCCCGCTCTGACCCATCTCATG 60.2 0.36
mm4_intergenic_Faim2|Gm5808_chr15_99554438_R GTCTCGTGGGCTCGGAAACAAGAGCGTGTGTCTAATTT 57.2 0.36
mm4_intergenic_Gm6027|Gm4746_chrX_90266734_F Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Gm6027|Gm4746_chrX_90266734_R Primer3: not found at this Tm N.d. 0.31
mm4_intergenic_Gm5899|Gm23884_chr7_95147132_F Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm5899|Gm23884_chr7_95147132_R Primer3: not found at this Tm N.d. 0.30
mm4_intergenic_Gm22337|Gm25347_chr3_115417935_F Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_Gm22337|Gm25347_chr3_115417935_R Primer3: not found at this Tm N.d. 0.29
mm4_intergenic_4921524L21Rik|Rab18_chr18_6651732_F Primer3: not found at this Tm N.d. 0.28
mm4_intergenic_4921524L21Rik|Rab18_chr18_6651732_R Primer3: not found at this Tm N.d. 0.28
mm3_intron_Fam196b_chr11_34408005_F TCGTCGGCAGCGTCATATAGCACCCAGGGGCAGA 60.1 0.27
mm3_intron_Fam196b_chr11_34408005_R GTCTCGTGGGCTCGGGCTTGGATGGAGATGGACCA 59.4 0.27
mm4_intergenic_Gm23176|Nt5e_chr9_88093262_F TCGTCGGCAGCGTCCAAGCACATGGGCAGATTCC 59.5 0.26
mm4_intergenic_Gm23176|Nt5e_chr9_88093262_R GTCTCGTGGGCTCGGGGCCTGTATTGCATGACAAGC 60.2 0.26
mm4_intron_Lmx1a_chr1_167776130_F Primer3: not found at this Tm N.d. 0.25
mm4_intron_Lmx1a_chr1_167776130_R Primer3: not found at this Tm N.d. 0.25
mm4_intron_Csmd2_chr4_128462076_F TCGTCGGCAGCGTCGGGTACACACACAGTTGCCT 60.1 0.24
mm4_intron_Csmd2_chr4_128462076_R GTCTCGTGGGCTCGGAGAACCAGGGAGGAGGTGG 60.2 0.24
mm4_exon_Tmem147_chr7_30728260_F TCGTCGGCAGCGTCAGGGCTAGACTGCATACCAGA 60.0 0.24
mm4_exon_Tmem147_chr7_30728260_R GTCTCGTGGGCTCGGGAGACTGCCTCCTTTTGCCT 59.9 0.24
mm4_intergenic_Gm11895|Gm24965_chr4_25909495_F Primer3: not found at this Tm N.d. 0.23
mm4_intergenic_Gm11895|Gm24965_chr4_25909495_R Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_Setbp1|Gm25824_chr18_79123550_F Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_Setbp1|Gm25824_chr18_79123550_R Primer3: not found at this Tm N.d. 0.23
mm3_intergenic_Jazf1|Gm25818_chr6_53341362_F Primer3: not found at this Tm N.d. 0.21
mm3_intergenic_Jazf1|Gm25818_chr6_53341362_R Primer3: not found at this Tm N.d. 0.21
mm4_intron_Gtdc1_chr2_44849383_F Primer3: not found at this Tm N.d. 0.21
mm4_intron_Gtdc1_chr2_44849383_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Tle3|Rplp1_chr9_61571165_F Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Tle3|Rplp1_chr9_61571165_R Primer3: not found at this Tm N.d. 0.21
mm4_intergenic_Cd44|Gm23731_chr2_102876060_F Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_Cd44|Gm23731_chr2_102876060_R Primer3: not found at this Tm N.d. 0.20
mm4_intergenic_A430078I02Rik|Pcbp1_chr6_86495381_F TCGTCGGCAGCGTCAAGACACACACACATGCCCT 59.8 0.20
mm4_intergenic_A430078I02Rik|Pcbp1_chr6_86495381_R GTCTCGTGGGCTCGGGTGTGTGAGGGGCTCCTTAC 60.0 0.20
mm2_intergenic_Zbed6/Zc3h11a|Lax1_chr1_133664754_F Primer3: not found at this Tm N.d. 0.18
mm2_intergenic_Zbed6/Zc3h11a|Lax1_chr1_133664754_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Rbfox1|Gm23476_chr16_8020670_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Rbfox1|Gm23476_chr16_8020670_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Lipg|Rpl17_chr18_74978834_F TCGTCGGCAGCGTCTCCGTTCTTCGTTTAGCTGGA 59.3 0.18
mm4_intergenic_Lipg|Rpl17_chr18_74978834_R GTCTCGTGGGCTCGGTGACAGTTGCAGTCGAGACC 59.9 0.18
mm3_intergenic_Gm14659|Gm14657_chrX_63263466_F Primer3: not found at this Tm N.d. 0.18
mm3_intergenic_Gm14659|Gm14657_chrX_63263466_R Primer3: not found at this Tm N.d. 0.18
mm2_intergenic_Gm15606|Opcml_chr9_28366732_F TCGTCGGCAGCGTCAGAAAACCAAAGACCTCAGGT 57.0 0.18
mm2_intergenic_Gm15606|Opcml_chr9_28366732_R GTCTCGTGGGCTCGGCAGCTTCCACCTTTGCAACC 59.9 0.18
mm4_exon_Lrrfip1_chr1_91101233_F TCGTCGGCAGCGTCCCGTAACATGCCTAGCCTGT 59.8 0.18
mm4_exon_Lrrfip1_chr1_91101233_R GTCTCGTGGGCTCGGATGCTTCGGTGTCCATGGAG 60.1 0.18
mm4_intergenic_5S_rRNA|Gcnt4_chr13_96912199_F Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_5S_rRNA|Gcnt4_chr13_96912199_R Primer3: not found at this Tm N.d. 0.18
mm4_intergenic_Gm14653|4930515L19Rik_chrX_46236526_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gm14653|4930515L19Rik_chrX_46236526_R Primer3: not found at this Tm N.d. 0.17
mm2_intron_Ptprf_chr4_118245709_F TCGTCGGCAGCGTCGACAGACCCTACCAGGCTCT 60.3 0.17
mm2_intron_Ptprf_chr4_118245709_R GTCTCGTGGGCTCGGGTGCTCCTCACACTGGTGTT 60.1 0.17
mm4_intergenic_Papss1|Gm26103_chr3_131771033_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Papss1|Gm26103_chr3_131771033_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gpd1l|Osbpl10_chr9_114940886_F Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Gpd1l|Osbpl10_chr9_114940886_R Primer3: not found at this Tm N.d. 0.17
mm4_intergenic_Zc3h12c|Gm25562_chr9_52286042_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Zc3h12c|Gm25562_chr9_52286042_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Sec22a|Gm25079_chr16_35374806_F Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Sec22a|Gm25079_chr16_35374806_R Primer3: not found at this Tm N.d. 0.16
mm4_intron_Gm12665_chr11_24904714_F Primer3: not found at this Tm N.d. 0.16
mm4_intron_Gm12665_chr11_24904714_R Primer3: not found at this Tm N.d. 0.16
mm4_exon_Pitpnm2_chr5_124139604_F Primer3: not found at this Tm N.d. 0.16
mm4_exon_Pitpnm2_chr5_124139604_R Primer3: not found at this Tm N.d. 0.16
mm4_intergenic_Zbed3|Aggf1_chr13_95344382_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Zbed3|Aggf1_chr13_95344382_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Ccdc121|Dnah14_chr1_181596815_F Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Ccdc121|Dnah14_chr1_181596815_R Primer3: not found at this Tm N.d. 0.15
mm4_intergenic_Gm7518|Gm15686_chr6_144725835_F TCGTCGGCAGCGTCTCTGCTGTCAGATCCTCAGGA 59.9 0.14
mm4_intergenic_Gm7518|Gm15686_chr6_144725835_R GTCTCGTGGGCTCGGGTGGGGTGTTTAGGAAGCGA 59.9 0.14
mm4_intergenic_Tbc1d9|Ucp1_chr8_83283261_F TCGTCGGCAGCGTCGGCAGGGAAAGTGGTTCAGT 60.1 0.14
mm4_intergenic_Tbc1d9|Ucp1_chr8_83283261_R GTCTCGTGGGCTCGGTGGAACACTCCCTACCCCTT 59.8 0.14
mm4_intergenic_Nt5m|Gm12714_chr11_59894621_F TCGTCGGCAGCGTCGGGCTGGGTTGGACAATGTA 59.9 0.13
mm4_intergenic_Nt5m|Gm12714_chr11_59894621_R GTCTCGTGGGCTCGGTGCCTGCTTCTAATCTGCCC 60.1 0.13
mm4_intron_Sh3bp5_chr14_31397456_F TCGTCGGCAGCGTCCTAGCACAGGTGACCACAGG 60.0 0.13
mm4_intron_Sh3bp5_chr14_31397456_R GTCTCGTGGGCTCGGGTGAGAACTCGGGACAGCTC 60.1 0.13
mm4_intron_Pomgnt2_chr9_121987558_F TCGTCGGCAGCGTCATTCCCTGCCATGCTGTTGT 60.2 0.13
mm4_intron_Pomgnt2_chr9_121987558_R GTCTCGTGGGCTCGGAGATGGCACTGTGCTCTGTC 60.0 0.13
mm4_intron_Slc15a5_chr6_138071802_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Slc15a5_chr6_138071802_R Primer3: not found at this Tm N.d. 0.13
mm4_exon_Plb1_chr5_32247457_F Primer3: not found at this Tm N.d. 0.13
mm4_exon_Plb1_chr5_32247457_R Primer3: not found at this Tm N.d. 0.13
mm4_intron_Fam134b_chr15_25892688_F Primer3: not found at this Tm N.d. 0.13
mm4_intron_Fam134b_chr15_25892688_R Primer3: not found at this Tm N.d. 0.13
mm4_intergenic_Gm7910|Khdrbs2_chr1_31873156_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Gm7910|Khdrbs2_chr1_31873156_R Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Got2|Gm22780_chr8_96178757_F Primer3: not found at this Tm N.d. 0.12
mm4_intergenic_Got2|Gm22780_chr8_96178757_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Cpb2_chr14_75256625_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Cpb2_chr14_75256625_R Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Gm14693|Gm14687_chrX_67634853_F Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Gm14693|Gm14687_chrX_67634853_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Dnm3_chr1_162337419_F Primer3: not found at this Tm N.d. 0.12
mm4_intron_Dnm3_chr1_162337419_R Primer3: not found at this Tm N.d. 0.12
mm4_intron_Vav3_chr3_109429776_F TCGTCGGCAGCGTCGACAGGTCACTCTGGGGTTG 59.9 0.12
mm4_intron_Vav3_chr3_109429776_R GTCTCGTGGGCTCGGGCCAAAGAGAAGCTCTGTATGC 59.6 0.12
mm3_intergenic_Syf2|Gm16225_chr4_135110215_F Primer3: not found at this Tm N.d. 0.12
mm3_intergenic_Syf2|Gm16225_chr4_135110215_R Primer3: not found at this Tm N.d. 0.12
mm4_exon_Wwc1_chr11_35870911_F TCGTCGGCAGCGTCGCTCACAGACGACTGACTCC 60.1 0.11
mm4_exon_Wwc1_chr11_35870911_R GTCTCGTGGGCTCGGTGGCCTCAAAGTAGCCTGTG 59.9 0.11
mm4_intergenic_Gm26235|Gm25392_chr18_18120781_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Gm26235|Gm25392_chr18_18120781_R Primer3: not found at this Tm N.d. 0.11
mm4_intron_Abcb4_chr5_8938196_F TCGTCGGCAGCGTCTTAGGGAGAGGGAGACTGGC 60.0 0.11
mm4_intron_Abcb4_chr5_8938196_R GTCTCGTGGGCTCGGACTCCAAATGAGCCCAAGGG 59.9 0.11
mm3_intron_Nrxn2_chr19_6506814_F TCGTCGGCAGCGTCGAGCTCATTGCACGGCATTC 60.2 0.11
mm3_intron_Nrxn2_chr19_6506814_R GTCTCGTGGGCTCGGTGCCTCCTCACAGACTTCCT 60.1 0.11
mm4_exon_Clic6_chr16_92498377_F TCGTCGGCAGCGTCAGAGTCTTGGGGCCCTCTAG 60.0 0.11
mm4_exon_Clic6_chr16_92498377_R GTCTCGTGGGCTCGGAAAACACCCTTCCTCGACGC 60.8 0.11
mm4_exon_Kctd20_chr17_28967530_F Primer3: not found at this Tm N.d. 0.11
mm4_exon_Kctd20_chr17_28967530_R Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Clec4a2|Clec4b2_chr6_123150949_F Primer3: not found at this Tm N.d. 0.11
mm4_intergenic_Clec4a2|Clec4b2_chr6_123150949_R Primer3: not found at this Tm N.d. 0.11
mm2_intron_Slc22a1_chr17_12673924_F TCGTCGGCAGCGTCGTAACCAGAAGCAGGCCAGG 60.6 0.11
mm2_intron_Slc22a1_chr17_12673924_R GTCTCGTGGGCTCGGCCCCACACATGCACATAGAGT 60.0 0.11
mm4_intergenic_Gm24781|Rps13-ps1_chr8_86980429_F TCGTCGGCAGCGTCTCTGAAGAGGAAGGACAGGT 57.2 0.10
mm4_intergenic_Gm24781|Rps13-ps1_chr8_86980429_R GTCTCGTGGGCTCGGTGACAGGAATCCACAGGTGTG 59.9 0.10
mm4_intron_T2_chr17_8415782_F Primer3: not found at this Tm N.d. 0.10
mm4_intron_T2_chr17_8415782_R Primer3: not found at this Tm N.d. 0.10
mm4_intron_Gucy2f_chrX_142107823_F TCGTCGGCAGCGTCAGTCCTGGGCTGCTTTTGAA 59.8 0.10
mm4_intron_Gucy2f_chrX_142107823_R GTCTCGTGGGCTCGGTCACTGTTCAGTAGTTGGCCTC 59.9 0.10
mm4_intergenic_SNORA7|Gm8420_chr1_51940406_F TCGTCGGCAGCGTCGCTGAACAACCGGATGTCCT 60.3 0.09
mm4_intergenic_SNORA7|Gm8420_chr1_51940406_R GTCTCGTGGGCTCGGTGACTGGTGTCCACTGTTAACA 59.4 0.09
mm4_exon_Bcl7b_chr5_135181420_F TCGTCGGCAGCGTCGAGGGACTGACCGGTAGAGA 59.7 0.09
mm4_exon_Bcl7b_chr5_135181420_R GTCTCGTGGGCTCGGCATCCAACAGAGGCAGGTCT 59.3 0.09
mm4_intron_Atp2b2_chr6_113850211_F TCGTCGGCAGCGTCCAGGGTGGCCAGTTAGGAAC 60.3 0.09
mm4_intron_Atp2b2_chr6_113850211_R GTCTCGTGGGCTCGGACCCGTTCTCCAGAGATGGA 59.9 0.09
mm3_exon_Bmp2k_chr5_97027811_F Primer3: not found at this Tm N.d. 0.09
mm3_exon_Bmp2k_chr5_97027811_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm6277/Cables1/Mir1901|Cables1_chr18_11865092_F Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm6277/Cables1/Mir1901|Cables1_chr18_11865092_R Primer3: not found at this Tm N.d. 0.09
mm4_intron_Clcnkb_chr4_141405248_F Primer3: not found at this Tm N.d. 0.09
mm4_intron_Clcnkb_chr4_141405248_R Primer3: not found at this Tm N.d. 0.09
mm4_intergenic_Gm24471|Cartpt_chr13_99866830_F TCGTCGGCAGCGTCATGGTCCCGCTTCTCCTACT 60.0 0.08
mm4_intergenic_Gm24471|Cartpt_chr13_99866830_R GTCTCGTGGGCTCGGGTTGGAAGTCCTCACCAGCA 59.8 0.08
mm4_exon_Gm12012_chr11_16440134_F Primer3: not found at this Tm N.d. 0.08
mm4_exon_Gm12012_chr11_16440134_R Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Sdk2|1700025D23Rik_chr11_114068582_F TCGTCGGCAGCGTCCCTGCCCATCCCATCAGAAA 59.7 0.08
mm4_intergenic_Sdk2|1700025D23Rik_chr11_114068582_R GTCTCGTGGGCTCGGGTGTCTCTGCTCCGATTGCT 60.1 0.08
mm3_intergenic_Slit3|Gm12123_chr11_35270766_F TCGTCGGCAGCGTCAGGGAGCTAGGCCATCAGAA 60.0 0.08
mm3_intergenic_Slit3|Gm12123_chr11_35270766_R GTCTCGTGGGCTCGGGCCCTCCAGTTTGTGTGGTT 60.7 0.08
mm4_intergenic_Cyp2j7-ps|Gm12658_chr4_96241515_F Primer3: not found at this Tm N.d. 0.08
mm4_intergenic_Cyp2j7-ps|Gm12658_chr4_96241515_R Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Frmd5|Gm14021_chr2_121696465_F Primer3: not found at this Tm N.d. 0.08
mm3_intergenic_Frmd5|Gm14021_chr2_121696465_R Primer3: not found at this Tm N.d. 0.08
mm4_exon_Ada_chr2_163727603_F TCGTCGGCAGCGTCGAGCCGTTCCAGAAGTTCCT 59.6 0.07
mm4_exon_Ada_chr2_163727603_R GTCTCGTGGGCTCGGGCTGCTTCCTTCCTTGTCCT 59.9 0.07
mm4_intergenic_Ncoa2|Tram1_chr1_13469194_F Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Ncoa2|Tram1_chr1_13469194_R Primer3: not found at this Tm N.d. 0.07
mm4_intron_Dtx4_chr19_12494191_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Dtx4_chr19_12494191_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm22792|Bcat1_chr6_144900299_F TCGTCGGCAGCGTCTGTGTAACTGGGGTTCTGCG 60.2 0.07
mm4_intergenic_Gm22792|Bcat1_chr6_144900299_R GTCTCGTGGGCTCGGAGGTGAACGTAAGCCTGCTC 60.0 0.07
mm4_intron_Abcg3_chr5_104977918_F Primer3: not found at this Tm N.d. 0.07
mm4_intron_Abcg3_chr5_104977918_R Primer3: not found at this Tm N.d. 0.07
mm4_intergenic_Gm26018|BC018507_chr13_70493710_F TCGTCGGCAGCGTCATCTTGCCAGAAGTCCAGCC 60.0 0.06
mm4_intergenic_Gm26018|BC018507_chr13_70493710_R GTCTCGTGGGCTCGGACTCATTTAGAAAACTCACTGTCCA 58.4 0.06
mm4_intron_Ttyh2_chr11_114704285_F Primer3: not found at this Tm N.d. 0.05
mm4_intron_Ttyh2_chr11_114704285_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Nfatc2_chr2_168487516_F TCGTCGGCAGCGTCTTTGTTTCTGGGAGGTGGGG 59.8 0.05
mm4_intron_Nfatc2_chr2_168487516_R GTCTCGTGGGCTCGGGCCTGGCTTGTAAATGCTGG 59.8 0.05
mm4_intergenic_Nphp4|Gm13172_chr4_152607339_F TCGTCGGCAGCGTCCAGTGTCTCCCCTGTTTCCC 59.9 0.05
mm4_intergenic_Nphp4|Gm13172_chr4_152607339_R GTCTCGTGGGCTCGGAATAGCACTGGTGAGCAGGC 60.3 0.05
mm3_intergenic_Actr3b|Gm21655_chr5_25884720_F Primer3: not found at this Tm N.d. 0.05
mm3_intergenic_Actr3b|Gm21655_chr5_25884720_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm6132|Gm26018_chr13_70125463_F TCGTCGGCAGCGTCTTAGAGGGACGAGTCTGGGG 60.0 0.05
mm4_intergenic_Gm6132|Gm26018_chr13_70125463_R GTCTCGTGGGCTCGGGGTGTGTGAGAGAGCCCAAA 59.8 0.05
mm4_intergenic_Gm13219|Gm13220_chr2_9448031_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm13219|Gm13220_chr2_9448031_R Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm13855|Gm14546_chr6_34233190_F Primer3: not found at this Tm N.d. 0.05
mm4_intergenic_Gm13855|Gm14546_chr6_34233190_R Primer3: not found at this Tm N.d. 0.05
mm4_intron_Reps2_chrX_162508936_F Primer3: not found at this Tm N.d. 0.04
mm4_intron_Reps2_chrX_162508936_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22331|Gm24937_chr1_113533122_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Gm22331|Gm24937_chr1_113533122_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Armc3|4930447M23Rik_chr2_19332161_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_Armc3|4930447M23Rik_chr2_19332161_R Primer3: not found at this Tm N.d. 0.04
mm2_intron_Coro1c_chr5_113907994_F Primer3: not found at this Tm N.d. 0.04
mm2_intron_Coro1c_chr5_113907994_R Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_D3Ertd751e|Gm25714_chr3_43592558_F Primer3: not found at this Tm N.d. 0.04
mm4_intergenic_D3Ertd751e|Gm25714_chr3_43592558_R Primer3: not found at this Tm N.d. 0.04
mm4_intron_Dgkg_chr16_22588253_F TCGTCGGCAGCGTCCAGATGCATCCCCAGTGTGT 60.0 0.03
mm4_intron_Dgkg_chr16_22588253_R GTCTCGTGGGCTCGGCTTGTCCCTGATGGAGACCG 59.8 0.03
mm3_intergenic_D3Ertd751e|Gm25714_chr3_42539290_F Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_D3Ertd751e|Gm25714_chr3_42539290_R Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_4930505K14Rik|Gm15407_chr5_148819647_F TCGTCGGCAGCGTCAATAGGCAGACACGGCTCTG 59.8 0.03
mm4_intergenic_4930505K14Rik|Gm15407_chr5_148819647_R GTCTCGTGGGCTCGGCCTCGGTAGTAAACTAGTAGTGGC 59.9 0.03
mm4_intergenic_Gm9755|Nat3_chr8_67522173_F TCGTCGGCAGCGTCCATGACCACTCAGGGACAGG 59.7 0.03
mm4_intergenic_Gm9755|Nat3_chr8_67522173_R GTCTCGTGGGCTCGGAGTTTTGGCTGTTGCACTGC 60.1 0.03
mm4_intron_Dhx34_chr7_16205535_F TCGTCGGCAGCGTCCGCTCAGACTTCACCTGGTT 59.9 0.03
mm4_intron_Dhx34_chr7_16205535_R GTCTCGTGGGCTCGGTAGGAGGGTCCCTTAGAGCG 59.8 0.03
mm4_intergenic_Rxra|2810430I11Rik_chr2_27786191_F Primer3: not found at this Tm N.d. 0.03
mm4_intergenic_Rxra|2810430I11Rik_chr2_27786191_R Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Sema3d|Gm23243_chr5_12700790_F Primer3: not found at this Tm N.d. 0.03
mm3_intergenic_Sema3d|Gm23243_chr5_12700790_R Primer3: not found at this Tm N.d. 0.03
mm3_intron_Kcnh1_chr1_192480188_F TCGTCGGCAGCGTCTGAGGCTCAAGGCACAAGAG 59.9 0.03
mm3_intron_Kcnh1_chr1_192480188_R GTCTCGTGGGCTCGGCACTAGGTGCTAAGTGGGCC 60.1 0.03
mm4_intergenic_Gm8840|Ikzf2_chr1_69524904_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm8840|Ikzf2_chr1_69524904_R Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50467576_F TCGTCGGCAGCGTCAACAAAGCCGTGGAGTGCTA 59.8 0.02
mm3_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50467576_R GTCTCGTGGGCTCGGCCCCAGCTTCTCGAATCGAA 59.8 0.02
mm4_exon_Zfp770_chr2_114196615_F TCGTCGGCAGCGTCTGAGTCTGCTTGGAACTCTG 57.1 0.02
mm4_exon_Zfp770_chr2_114196615_R GTCTCGTGGGCTCGGTGTCCCTTTTCAGTGTTCGGA 59.5 0.02
mm3_intergenic_Pkdcc|Gm24240_chr17_83242326_F Primer3: not found at this Tm N.d. 0.02
mm3_intergenic_Pkdcc|Gm24240_chr17_83242326_R Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23370|Mgat5_chr1_127364290_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gm23370|Mgat5_chr1_127364290_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Zfp395_chr14_65374203_F TCGTCGGCAGCGTCCAGTGAGCTCATTTGCTGCC 59.8 0.02
mm4_intron_Zfp395_chr14_65374203_R GTCTCGTGGGCTCGGCAAAGTGGCATGGGTGGTTG 59.9 0.02
mm4_intergenic_Gldc|Mbl2_chr19_30200819_F Primer3: not found at this Tm N.d. 0.02
mm4_intergenic_Gldc|Mbl2_chr19_30200819_R Primer3: not found at this Tm N.d. 0.02
mm4_intron_Ppargc1a_chr5_51552686_F TCGTCGGCAGCGTCTATCACTGCACCACACGGAC 60.0 0.02
mm4_intron_Ppargc1a_chr5_51552686_R GTCTCGTGGGCTCGGGGAGGATCGGAGCTGCTAAC 59.9 0.02
mm4_intergenic_Gm22420|Ergic1_chr17_26596249_F TCGTCGGCAGCGTCAATACTATCCCCAGGCCCCA 59.7 0.02
mm4_intergenic_Gm22420|Ergic1_chr17_26596249_R GTCTCGTGGGCTCGGGGGAGCGCTATTCCTGGAC 59.9 0.02
mm4_intergenic_Mgat5b|Gm11728_chr11_117026935_F TCGTCGGCAGCGTCCCCAAGCCATTGCCCTATGA 60.1 0.01
mm4_intergenic_Mgat5b|Gm11728_chr11_117026935_R GTCTCGTGGGCTCGGTACAGAACAGCACCAGTGGG 59.6 0.01
mm4_intergenic_Gm2001|Gm2016_chr12_87860399_F Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_Gm2001|Gm2016_chr12_87860399_R Primer3: not found at this Tm N.d. 0.01
mm4_intergenic_H6pd|Gm13067_chr4_150029512_F TCGTCGGCAGCGTCACTGTCTGGTGGGCAGAGA 60.1 0.01
mm4_intergenic_H6pd|Gm13067_chr4_150029512_R GTCTCGTGGGCTCGGGAGGGAGGGTGGAAGACTCT 59.9 0.01
mm4_intron_Mark1_chr1_184918115_F TCGTCGGCAGCGTCGATGGGTGAGCTCTGTCTCC 59.5 0.01
mm4_intron_Mark1_chr1_184918115_R GTCTCGTGGGCTCGGGCTAGAGGTGGAGGTGGGAT 60.3 0.01
mm3_intron_Cd209g_chr8_4136461_F Primer3: not found at this Tm N.d. 0.00
mm3_intron_Cd209g_chr8_4136461_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Foxn3|Gm26839_chr12_99517256_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Foxn3|Gm26839_chr12_99517256_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Alk_chr17_72275799_F TCGTCGGCAGCGTCGCGAAGATGTTTCAACTGGTCC 60.0 0.00
mm4_intron_Alk_chr17_72275799_R GTCTCGTGGGCTCGGGCGGCAGACTCTCTGGTATC 59.9 0.00
mm4_intron_Disc1_chr8_125108709_F TCGTCGGCAGCGTCCATGAGACCAGGGCCTGATG 60.1 0.00
mm4_intron_Disc1_chr8_125108709_R GTCTCGTGGGCTCGGGGGAAAATGCCATTGCGAGA 59.1 0.00
mm4_exon_Ucp2_chr7_100493842_F TCGTCGGCAGCGTCAAGGGTTGTTTGTGCCTGGA 60.0 0.00
mm4_exon_Ucp2_chr7_100493842_R GTCTCGTGGGCTCGGCCAGTCTCCCTGTGTCCCTA 59.9 0.00
mm4_intron_Dock8_chr19_25190900_F TCGTCGGCAGCGTCCCATCTAGACCGCTGCTGAG 59.9 0.00
mm4_intron_Dock8_chr19_25190900_R GTCTCGTGGGCTCGGTGCCTTCCCAACACCTTTCT 59.4 0.00
mm4_intron_Lsamp_chr16_41661493_F TCGTCGGCAGCGTCTGGAGTAGGTAGGTGAGCCC 60.0 0.00
mm4_intron_Lsamp_chr16_41661493_R GTCTCGTGGGCTCGGACCCCTCTGACCTCAACAGT 60.1 0.00
mm4_intron_Aspm_chr1_139465683_F Primer3: not found at this Tm N.d. 0.00
mm4_intron_Aspm_chr1_139465683_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4763|Cd177_chr7_24738804_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm4763|Cd177_chr7_24738804_R Primer3: not found at this Tm N.d. 0.00
mm4_intron_Mrpl35_chr6_71823360_F TCGTCGGCAGCGTCAGGAGACCAGATACTCGATGCT 60.1 0.00
mm4_intron_Mrpl35_chr6_71823360_R GTCTCGTGGGCTCGGAGGTTCAACCACCTCTCTCA 57.8 0.00
mm4_intron_Pde11a_chr2_76003199_F TCGTCGGCAGCGTCGAATGGCTGCTGACCCAGAA 60.3 0.00
mm4_intron_Pde11a_chr2_76003199_R GTCTCGTGGGCTCGGACTCCGATGTGTAAAGGGTGG 59.7 0.00
mm4_intron_Mak_chr13_41052199_F TCGTCGGCAGCGTCGGGCAGGATTCAGGAAACCA 59.9 0.00
mm4_intron_Mak_chr13_41052199_R GTCTCGTGGGCTCGGTTCAGAAGCCAGTGCCTGAG 59.9 0.00
mm4_intron_BC030867_chr11_102251721_F TCGTCGGCAGCGTCTAAAGGCCTTCCTGGGAGCT 60.5 0.00
mm4_intron_BC030867_chr11_102251721_R GTCTCGTGGGCTCGGCCTCGGGTCTCACATCATCC 59.6 0.00
mm4_intron_Ap2a1_chr7_44922722_F TCGTCGGCAGCGTCGGCACAGGGCTTGAGATAGG 60.1 0.00
mm4_intron_Ap2a1_chr7_44922722_R GTCTCGTGGGCTCGGCCTGGGCTCTGTGAAGGC 60.0 0.00
mm4_intergenic_Rtn4rl1|Rpa1_chr11_75268679_F TCGTCGGCAGCGTCAAGGCTGAGCTTTGTCCAGG 60.2 0.00
mm4_intergenic_Rtn4rl1|Rpa1_chr11_75268679_R GTCTCGTGGGCTCGGTGTCATCAGCCTCCGATCCT 60.3 0.00
mm4_intergenic_Pex5l|AC113989.1_chr3_33224543_F TCGTCGGCAGCGTCCCCTGATTGCTGGGTCTACC 59.8 0.00
mm4_intergenic_Pex5l|AC113989.1_chr3_33224543_R GTCTCGTGGGCTCGGCAGCAGATTGGTGGGGAAGG 60.6 0.00
mm4_intergenic_Olfm2|Col5a3_chr9_20746420_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Olfm2|Col5a3_chr9_20746420_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Golga5|Chga_chr12_102552926_F TCGTCGGCAGCGTCTGGCAAAGTGAGAATCGGGG 60.3 0.00
mm4_intergenic_Golga5|Chga_chr12_102552926_R GTCTCGTGGGCTCGGGCCAAAGGCCACCTCTAGTT 59.9 0.00
mm4_intergenic_Gm27039|Adcyap1_chr17_92763312_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm27039|Adcyap1_chr17_92763312_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26474|RP24-351J24.2_chr5_120376625_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm26474|RP24-351J24.2_chr5_120376625_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm16397|Gm10344_chrX_134734216_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm16397|Gm10344_chrX_134734216_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12906|Gm12907_chr4_107762062_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12906|Gm12907_chr4_107762062_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12541|Fgf2_chr3_37363798_F Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_Gm12541|Fgf2_chr3_37363798_R Primer3: not found at this Tm N.d. 0.00
mm4_intergenic_A330048O09Rik|Zfp169_chr13_48285474_F TCGTCGGCAGCGTCGGAGAAGCCGTTGTTCCTCT 59.6 0.00
mm4_intergenic_A330048O09Rik|Zfp169_chr13_48285474_R GTCTCGTGGGCTCGGGGAGTGTCAGTTTCCCAGCA 59.8 0.00
mm4_exon_Lmtk3_chr7_45803873_F TCGTCGGCAGCGTCAGATGAGTCCCCTGATCCCC 60.1 0.00
mm4_exon_Lmtk3_chr7_45803873_R GTCTCGTGGGCTCGGTCTGGGCCCAAAAGAGTTCG 60.2 0.00
mm3_intergenic_Sntb1|Has2_chr15_56071167_F Primer3: not found at this Tm N.d. 0.00
mm3_intergenic_Sntb1|Has2_chr15_56071167_R Primer3: not found at this Tm N.d. 0.00

Off-target amplicon sequences with primers

These only list off-targets that have primers in the table above. Primers underlined, off-targets in bold.

ontarget_mm0_exon_5730457N03Rik/Evx1_chr6_52313620 TTGCAGCAGGAGGATGACAGCTTAGAAAGAAGAGGGCAATGGGGCTTCCTCCCAGAGGCGGTGCGGCACAGAGGAGCGCT
CGCATCACAAGGTGACCCTAGCTCCCCACTGCCATCT
mm4_intron_Atp2b4_chr1_133771957 ATCCTGTCCCGGTTAGCTCAGAGGAGTCAGAGGAGAAAGCTTAGAGACTTCCTCCCAGAGGTGGCTGTGAGGCGTGCTGA
GGGCATATGTGACGTGATGCCTGTGT
mm4_intergenic_Terf2|C630050I24Rik_chr8_107118266 AGTGACTCTGCAAAGGAGCCCCAGTTTCCAAGGTCACCTCCCTCTCTGGGAGGGAGCCCTAAGCACCCAGAATGGTCCCC
AGGATGAGATAATCAAGGCACGCCTAAGAAACATCTGGGATACGTGTACACCCACC
mm2_exon_Sh2b3_chr5_121836071 TATTCACCCCTCCTGGCTCAGATGGCGTTTCCTCCCAGAGGAGGCCTGGGGAACAAGGTTCAGA
mm3_intron_Elovl1_chr4_118429291 CCAACTTTGAGGAGGAGGGGACCTAGCCCATGAGGCTCCCTCCCAGAAGAGGGAAAGATTCCTGAGACCTCCCCCACTCT
CCCACCTCCTCACATACCACTGGTGTCCTCACCCCTCCCTGGTAGGGAAGGGTGATGC
mm3_intergenic_5031439G07Rik|Upk3a_chr15_84996341 TCACAATCAACAGGCACCCTTTGGGCATGGGTCTTCCTCCCAAAGAAGGAGTCGGCAGCCCCAGGGCAGGACAGAGCGGG
TGCTGAAGCTGAGCAGGCACAGCCCACCCAGCAGCCGGTGAGGAGGTCTGCCTGGACAA
mm4_intron_Nfic_chr10_81424584 CTGCCTTGGGACACTTCTCATCGTCTCTGATCCCATCCCCCTCTGGAAGGAGACCCCAAACGTCAACCCTGCACTGCCTC
AGTTTCCCCACTGCCAGGATGGAAGCAGCGAGCCGCAAAGCTATGAGGAG
mm4_intron_Hydin_chr8_110361251 GACAAATGTCTGGGCGGAGAAACACAGGGCTATTCCCAGGCCCCAGGTTCTGAACAGGAAGATACCCCTCTTCTGGGAGG
AAGTTCCGT
TACAATTGTTCTTCTTAAAAAAAAAAAAAAAGCACCCCTCCCCTCCCAAAA
mm2_intergenic_Fnip2|Gm3513_chr3_79530536 CCCCATAGCTCAGCAGTAGCATGCCGCAGTCAGCCCTGGGGCTGCCTCCCAGAGGTGGAGCTCCACTTCCCCACCACTCA
ACCCTTCTTTCCTGACAGGAGCAGGTAAGAGCACACGT
mm4_intergenic_Gm23735|Fndc3a_chr14_72465504 TCTGCTTGGACTGTGTAGGTATCTGAATGCCCCTTCTGGGAGGAGGCTCCTTCATAGTCCTTCAAAGTTACTTAACTGAT
ACACTGATGAGTGCTCACTGAATTACAGAACCCACGTCTTCCAGTGAC
mm4_exon_Utp18_chr11_93862003 CCCTGCCCTTTTCATTCCCTAAGGCAAAGTATCCACCTCTGGGAGAAAAATCCATGGTTTGAACACGAGAAAGAGTACTC
TTTTTAAAAACTGGAAAGTTAGAAAATACTGTACAGGAAGGAAGATGAACCTGCAGGAGA
mm4_intergenic_Mirlet7b|Ppara_chr15_85724462 GTCTGTCCTAGCTGGCCAACGTGTTGGGCTTCCTCCCAAAGAAGGCAGGGGTGACCAGGCAAAGAGGTGCAGCTCTGGCC
TTCATGTCTCTCAGC
mm4_intron_Fhit_chr14_9841146 CTCAGCATCGACAAATGGCATAGGAAATTCATTTTTAATGCACTCATTTAGGGCTGCCTCCCAGAGAGGGAGAGGCCTGC
ATATATTAATAAAGGCCAAGAAATAAATGGCATAGCTGTCCATACTTCACCCTGA
mm4_intron_Tmc1_chr19_20894640 GAACTGTCCAGAGCAGCTGTGGCCGTAGTTCTCTTGCCTTGAGAATGCAGGTCCAACAACAGCAGAACCATGCCCACTTC
TGGGAGGAAGCGCTAA
TTGGTCACTTGCCATTTACAGAGA
mm4_intergenic_Tbr1|Gm13555_chr2_61814573 CTTGGAGGTGACTGGCGAATCTTTATAAATGCAAACTTGGACGGGCCTTCCTCTCAGAGAGGGAGGTCTCAGGGCATTGT
TCCGGGTGAGGTGGAGAAAGAC
mm4_intergenic_Faim2|Gm5808_chr15_99554438 CCGCTCTGACCCATCTCATGGCCAGAGCCTGTACGGAAGCAGGAAATGATATTACACTAGGAAGGAGTCTGACCCTGGTC
CCCTTCTGGGAGGAAGACTCAG
CAGGGTAAATAAATTAGACACACGCTCTTGTTT
mm3_intron_Fam196b_chr11_34408005 ATATAGCACCCAGGGGCAGAGCAGGGAATCTAGTGGATTTAAGCTGTAAAACGCCTCTAAGAGTTTGAGAAGATAATGGG
GCTTTTCCCCAGAGGTGG
AGGGGTGGTCCATCTCCATCCAAGC
mm4_intergenic_Gm23176|Nt5e_chr9_88093262 CAAGCACATGGGCAGATTCCCTGGAGCTTCCTACCAGAGATGGAAGAGTCCAGTACATTCATATTGCTTGTCATGCAATA
CAGGCC
mm4_intron_Csmd2_chr4_128462076 GGGTACACACACAGTTGCCTGTCTGGGCATAGTTTGTGGCCAGACAGAAAACACTATTACTTCAATACATTGGATATTAC
ATGGGGTTGGGGCATCCAACCAGAGGTGGGGGGAGGTACCCCACCTCCTCCCTGGTTCT
mm4_exon_Tmem147_chr7_30728260 AGGGCTAGACTGCATACCAGACTGATGTTGGAGTCAATGCTCATCTGGATATATTTCCAGTCAAATTCAATGCCTCGGGC
TCCCACCCAGAGGGGG
ATGCATCTGAGGAAGGCAAAAGGAGGCAGTCTC
mm4_intergenic_A430078I02Rik|Pcbp1_chr6_86495381 AAGACACACACACATGCCCTCCACCTCTCTAAGGAGGCTCTCTTCCCCCACATACCCACCACCTCTGTAAGGAGGCCCCC
T
ACCCCCACATACCCACCACCTCTGTAAGGAGCCCCTCACACAC
mm4_intergenic_Lipg|Rpl17_chr18_74978834 TCCGTTCTTCGTTTAGCTGGATTAACTCTTCATGGAGGAGACAATGGCGCGTCCACCCAGGGGTGGATCCCATTCTTTTG
ACCTTCAATGGGTCTCGACTGCAACTGTCA
mm2_intergenic_Gm15606|Opcml_chr9_28366732 AGAAAACCAAAGACCTCAGGTAGGGAGTTTCTGCCTCTGGAAGGAAGCCCCACATGGAGTCATAGAGAATCAATTCCATG
GTTGGTGAGAGAAGTGAAAGGTTGCAAAGGTGGAAGCTG
mm4_exon_Lrrfip1_chr1_91101233 CCGTAACATGCCTAGCCTGTCTGCAGCCACACTGGCATCTCTGGGTGGGACTTCCTCCCGGAGAGGGAGTGGAGATACCT
CCATCTCCATGGACACCGAAGCAT
mm2_intron_Ptprf_chr4_118245709 GACAGACCCTACCAGGCTCTGGGGTCAGGGTCCACCCAGCTCACAAACCCACCGAGGCACACAAGCCACTCCAGCCCCAC
CACTGTGAGGAAGCCCCAT
GCCTCGCCAACACCAGTGTGAGGAGCAC
mm4_intergenic_Gm7518|Gm15686_chr6_144725835 TCTGCTGTCAGATCCTCAGGAACTGGATGGGGCTCCCTCTCACAGATGGGCTGTCAATATTTAATTCCATACAGGGTCAG
AGTCTTATGTGTAGACTGGTAAGTTTCGCTTCCTAAACACCCCAC
mm4_intergenic_Tbc1d9|Ucp1_chr8_83283261 GGCAGGGAAAGTGGTTCAGTTTGATTAGAAGGCACCAAAACATAAGTGTGTATATGCAAAATGCAACCCATTTGTTTTGG
TAAGAGATTCTAATTGGGGCTGCCTCACAGATGGGGAAGGGGTAGGGAGTGTTCCA
mm4_intergenic_Nt5m|Gm12714_chr11_59894621 GGGCTGGGTTGGACAATGTAGGTAAAACTGAGCCCTAAATTCCACCTCTGGGAGGGAGTACAATCAGAGAGTCAAATTAG
AAGCTTGTCAGAGTCAAAGAGCTTGAGAGCCAGGTCAAAGGGCAGATTAGAAGCAGGCA
mm4_intron_Sh3bp5_chr14_31397456 CTAGCACAGGTGACCACAGGTCCCTCCAACTTGCCCGCCCCTCCTTTATATTCATCATTCTTCCCCACCCCTCCTCCCCC
CGCCTCTGGGAGCCAGACCCAG
AAGGAGCTGTCCCGAGTTCTCAC
mm4_intron_Pomgnt2_chr9_121987558 ATTCCCTGCCATGCTGTTGTGTGCAACAGGCTGTCCAGTGCAATGGGCCTTCCTTCCAGGAGAGGGGAGGAGCAGAGTGC
CTAGGACAGAGCACAGTGCCATCT
mm4_intron_Vav3_chr3_109429776 GACAGGTCACTCTGGGGTTGTTGCTTGCTGATCCTTCTCTTAGGGCAGGATCCTTCTCTCAGGCAGAAGCCAACCTGCTC
TGTGGGGAAGCTCCAT
TGTCCTTTTAGATGGCATACAGAGCTTCTCTTTGGC
mm4_exon_Wwc1_chr11_35870911 GCTCACAGACGACTGACTCCCCGGAACCCACCTCTGTGCTGAAGCCTCATACACGCCACTGTCCCCGGCTACTGACTCGT
CGGACACTGCAGCGGACACACAGGCTACTTTGAGGCCA
mm4_intron_Abcb4_chr5_8938196 TTAGGGAGAGGGAGACTGGCACCGAGGCTTGGAGTGGAAGTGAAACCTAATTGTGGCTTCATCCCAGAGCAGGTGGGGCT
GGGCCCTTGGGCTCATTTGGAGT
mm3_intron_Nrxn2_chr19_6506814 GAGCTCATTGCACGGCATTCCGCATCCTGGTAACCTGTGACTAGTCGCCTGGGCATGGAAGTTTAGGGGATGATGCTTCC
TCCCAGAGCAGG
AAGGGCAGGAAGTCTGTGAGGAGGCA
mm4_exon_Clic6_chr16_92498377 AGAGTCTTGGGGCCCTCTAGATTTTGCATCAAGCGAAACCTTCGCTTCTAGAACCGCGAGTGGGGTGTCCTCCCGGAGGG
GG
CACGTCAGCCCCTGCCCCTCACCCTGAGCAGCGTCGAGGAAGGGTGTTTT
mm2_intron_Slc22a1_chr17_12673924 GTAACCAGAAGCAGGCCAGGGGGAAGAGACACTGGGCTTCCTCCCAGAGGCAGCCCTGCCTGTATTGACGTTAAAACCTT
TAAGGAAAAGGGAAGAAAGAAGGAAGGGATCCTAAGAAACTCTATGTGCATGTGTGGGG
mm4_intergenic_Gm24781|Rps13-ps1_chr8_86980429 TCTGAAGAGGAAGGACAGGTTAAACATCAGTTCTAACTCTTTGACCCACTAATACAAAGGGGCTGCTTCCCAGATGGGGT
GGCAGTCACACCTGTGGATTCCTGTCA
mm4_intron_Gucy2f_chrX_142107823 AGTCCTGGGCTGCTTTTGAAAGTGGAATGTCCTTTACCCCTTATGGAAGGAAGCCCCTTCCACAATATAAGGACTCAGTG
AGTATAATCTCAGAGAGGCCAACTACTGAACAGTGA
mm4_intergenic_SNORA7|Gm8420_chr1_51940406 GCTGAACAACCGGATGTCCTACCCATTCTATGCTATAGGGCTTCCTCTCATAAGGGGAGACCTCTTGTTAACAGTGGACA
CCAGTCA
mm4_exon_Bcl7b_chr5_135181420 GAGGGACTGACCGGTAGAGATGTGGAGAACACCGGAGAGAGGCTCTTCCGGGACGGAGGGGCTTTCGCCACCTTTGGGCA
GAAGACCCAT
GGGAGATGCATCCTGTGCCTGAGGCAGACCTGCCTCTGTTGGATG
mm4_intron_Atp2b2_chr6_113850211 CAGGGTGGCCAGTTAGGAACCTGGGGCTTCTTCCCAGTGTTGGCCCTAGTGTCAACTTCCCCCATGTCCCTGGTCTCCAG
ATGAGCTGCCAATGGCAGTGGTAAGGAGAAGCATCCATCTCTGGAGAACGGGT
mm4_intergenic_Gm24471|Cartpt_chr13_99866830 ATGGTCCCGCTTCTCCTACTGCCCTTGGCCATTCTATTCCAGGAGAGTAGACAGCACTGGTCAGACATCAATTCAAAATC
TCTATGTGATGTGACATTGGGACTCCTCCCACAGGAGGATGCTGGTGAGGACTTCCAAC
mm4_intergenic_Sdk2|1700025D23Rik_chr11_114068582 CCTGCCCATCCCATCAGAAACAGCTGTTCTTTAGGGTGTTGTTACAGGGCTTGCTCACAGAGGGGGCCTGAGAGTCAGTT
TTGCAGCAATTGCTTCTTAGCCAGCAATCGGAGCAGAGACAC
mm3_intergenic_Slit3|Gm12123_chr11_35270766 AGGGAGCTAGGCCATCAGAACCCTGTCCTCACAGTGCTCCGCAGCCCAGGGATGGCTCAGAAGAGAGAGCTGGGGCTTGC
TCCCAGAGAAAG
GCAAAAAGGGAACCACACAAACTGGAGGGC
mm4_exon_Ada_chr2_163727603 GAGCCGTTCCAGAAGTTCCTTCTTCTCTTCCTCTGGGAGGAAGCTTGACTTCGCTGCGTTGATGTTCTAGAAGGGGGAAA
CATGGAGTCAGGACAAGGAAGGAAGCAGC
mm4_intergenic_Gm22792|Bcat1_chr6_144900299 TGTGTAACTGGGGTTCTGCGGGAAAACCAAGAAAGAAAAAGAAGCAGAGCCTCACTCCCTAAAGAGCATGGGGGTCCCTC
ACAGTGGAGG
GAGCAGGCTTACGTTCACCT
mm4_intergenic_Gm26018|BC018507_chr13_70493710 ATCTTGCCAGAAGTCCAGCCAAGTGCTGAAAGTAAAAGCAAATTTGCCCAAAATCTACCCACTGTGGTAGGAAGCCCCAA
GATTCCTGTCTGTTTGGACAGTGAGTTTTCTAAATGAGT
mm4_intron_Nfatc2_chr2_168487516 TTTGTTTCTGGGAGGTGGGGAGGGGGATCCCTTTCCACTACTGAACTGGAATCCACTCTCAAGAAGAGTTGGGACTCCCT
GGGGTCCCCAAGGGGCTTCATCCAAGGGGAGGAGGGTTTCCCAGCATTTACAAGCCAGGC
mm4_intergenic_Nphp4|Gm13172_chr4_152607339 CAGTGTCTCCCCTGTTTCCCGGGGACACCTCATTTACCCTCCTTCCAAGTTTCTGCTCTCCAGCTGTTACAGCCCCAGAA
TTCTGGGGGTGCCTCCAAGAGGCGGGTGTTTGCCTGCTCACCAGTGCTATT
mm4_intergenic_Gm6132|Gm26018_chr13_70125463 TTAGAGGGACGAGTCTGGGGCAAGAGTTGGTTAACAATGACGCTTCTTCCCACAGGAGGCCACAGAGTCACTGTGGTCTT
TCTGTGGGATGTTTTGTTTTGGGCTCTCTCACACACC
mm4_intron_Dgkg_chr16_22588253 CAGATGCATCCCCAGTGTGTACGACATGACTGTCTAGAACCTTGGTCTTCCTCTGGGAGGAAGCCGGGGCCTTAGACAGG
TGAGCACTTACATTCTAGCTTATCCTGAGGTCGCCCGGTCTCCATCAGGGACAAG
mm4_intergenic_4930505K14Rik|Gm15407_chr5_148819647 AATAGGCAGACACGGCTCTGAGGTTCCACCCACAGTGTCCTTTGAAAGAACCTCCTCTCGGAGGAAGTCTCTTGGCAATT
TGCCACTACTAGTTTACTACCGAGG
mm4_intergenic_Gm9755|Nat3_chr8_67522173 CATGACCACTCAGGGACAGGCCACCTCTGGCAGGAAGCACCCACTCTTCACCGCAGTGCAACAGCCAAAACT
mm4_intron_Dhx34_chr7_16205535 CGCTCAGACTTCACCTGGTTGAGGGGCAGACACCTCAGAGTGAGGACCTGGCCAGCTGTGGGAGCAAGCCCCAGCACGGG
AGCCTCTGAAGCAGGGCGGGCTGGGGGCCGCTCTAAGGGACCCTCCTA
mm3_intron_Kcnh1_chr1_192480188 TGAGGCTCAAGGCACAAGAGAATTGGGCTTTCTCCCATAGGGAGGGGCAGGAGGCCCACTTAGCACCTAGTG
mm3_intergenic_Rpl10-ps3|1600029D21Rik_chr9_50467576 AACAAAGCCGTGGAGTGCTACAGTCACCTCTGGGAGGAAGTGGCATTGAACCAGGGACCTGGAAACTGAGAAGCCTTCGA
TTCGAGAAGCTGGGG
mm4_exon_Zfp770_chr2_114196615 TGAGTCTGCTTGGAACTCTGCCACCTCTGGCAGGAAGACACTTGTGTCCATTGAGAATCTGCTCTGACTCAAAACATTCT
TCACACTCCGAACACTGAAAAGGGACA
mm4_intron_Zfp395_chr14_65374203 CAGTGAGCTCATTTGCTGCCGGGGCTGATATGTGCCTTCTCCTCTGGTGGCCCTGCTGTTGGGGGAAGCCCCATCCTGGA
TCACTGCCCCAGGGCCCCTTTCCCCTTTCCTGCCACAACCACCCATGCCACTTTG
mm4_intron_Ppargc1a_chr5_51552686 TATCACTGCACCACACGGACATCTTATTTAATCAAATCACGTGTGGCACTTGACCCTCCCCCTTACCCTACGTGCCCCCG
CTACCGGGAGGAAGCACCAT
TGTTAGTTAGCAGCTCCGATCCTCC
mm4_intergenic_Gm22420|Ergic1_chr17_26596249 AATACTATCCCCAGGCCCCACCATCCAGCCCCTGTTTGTTGGTTTTAAACCTGTTGGAAGCCCGCCCCTGGCAGGAAGCC
ACAA
GCATGAGAATCTAGTCCAGGAATAGCGCTCCC
mm4_intergenic_Mgat5b|Gm11728_chr11_117026935 CCCAAGCCATTGCCCTATGAGGGACAATCTGGGGGAAGATGGGGCTTCCTCACGGGTGAGGTCCTACTGTGACTGTCACA
GAGCCCCCGGCCCACTGGTGCTGTTCTGTA
mm4_intergenic_H6pd|Gm13067_chr4_150029512 ACTGTCTGGTGGGCAGAGAGGAGCCGGCTTCTTTCCCCTCTGGGAGAAAGCACCGAGGGCCACCTGTCACTGTCACCTTT
GCCTCTGGCTCTGAACACAAAGCTTCAGGAGTCCAAAGAGTCTTCCACCCTCCCTC
mm4_intron_Mark1_chr1_184918115 GATGGGTGAGCTCTGTCTCCCAGGGACTTTGTTCCTTCTACTCTGCCCTATGCACTGCTGCTTCCCCTCTGGGAATGTTA
CTGACACGCAGCTGAAGGGGCTTCCTGACACAGGCGGATCCCACCTCCACCTCTAGC
mm4_intron_Alk_chr17_72275799 GCGAAGATGTTTCAACTGGTCCTGAAGATCTGATGAGTAATTCTGTCCAGGAAGATGTGGCTTCCTGACAGACGGGGAAC
CTTCCCAGTGATTTAAATACGAGTTTGGATACCAGAGAGTCTGCCGC
mm4_intron_Disc1_chr8_125108709 CATGAGACCAGGGCCTGATGGCCATGGAATGGGTCTTCTTGCCATAGGGGGCCACTTGAGGAAATCCAGAGTTCTCGCAA
TGACTTTCTCTCGCAATGGCATTTTCCC
mm4_exon_Ucp2_chr7_100493842 AAGGGTTGTTTGTGCCTGGAGGAGCCTTGGAGTTTGTTTGGCCTTTGTTACACTATCTTATGTGGCTGCCTGCCGGAGGT
GG
AAGTAGGGACACAGGGAGACTGG
mm4_intron_Dock8_chr19_25190900 CCATCTAGACCGCTGCTGAGTGATGTAGCTTCCTCAGAGAGGAGGAGTCACAGAGCCTGCCCTGGAGACTGTCCATAGAA
AGGTGTTGGGAAGGCA
mm4_intron_Lsamp_chr16_41661493 TGGAGTAGGTAGGTGAGCCCCACATTGCTTCCTCCCAGAGGTGAAGAAATCACTGTTGAGGTCAGAGGGGT
mm4_intron_Mrpl35_chr6_71823360 AGGAGACCAGATACTCGATGCTTTACACACTTGTTTAACCCACAGGTAGGAGGAAGNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGTTGAGAGAGGTGGTTGAACCT
mm4_intron_Pde11a_chr2_76003199 GAATGGCTGCTGACCCAGAAGCCGCAGGCTTTGATGGATGGGGCTTCGTGGAAGAGGTGGTTGGGACACGTAACTAAAAT
GTAGCCACATGGTAATAATCCACCCTTTACACATCGGAGT
mm4_intron_Mak_chr13_41052199 GGGCAGGATTCAGGAAACCACGAAGCCTTTCCTTGTGAGTCAGCTTTAAGGGGCTTCTTCCCTGAAGTGGGCTCAGGCAC
TGGCTTCTGAA
mm4_intron_BC030867_chr11_102251721 TAAAGGCCTTCCTGGGAGCTGTCTGGAGGGTGATTTCATTCACTTTTATAATAGCATGCCTGAAGGGGGAGGAACGTTTA
TAGAGAGCCGCCTCAGGGAGGAGGCCGCACAGTTAGCAGGATGATGTGAGACCCGAGG
mm4_intron_Ap2a1_chr7_44922722 GGCACAGGGCTTGAGATAGGAGACAGCCCCAGCCTGCAGCTCCCTCTGATAAGGCTTCCCTGCTCAGGGAGGGAGCACCA
T
GCCTTCACAGAGCCCAGG
mm4_intergenic_Rtn4rl1|Rpa1_chr11_75268679 AAGGCTGAGCTTTGTCCAGGCTGGACAGTGTGGCCCAGGACATTGGGTGTATTTTTGGAGCTTCCTCCCCCAGGGGGTAG
AAGGATCGGAGGCTGATGACA
mm4_intergenic_Pex5l|AC113989.1_chr3_33224543 CCCTGATTGCTGGGTCTACCTGGAGCACAGACAGAAGAGGTAAACTACATACTGTCCTCTGAACTCTAGGGCTTCCCCCC
TGAGGAGG
CCTACCTGTCCCCAGGAACATATAGGTTAATCCCTTCCCCACCAATCTGCTG
mm4_intergenic_Golga5|Chga_chr12_102552926 TGGCAAAGTGAGAATCGGGGTTTAATTCTTACGGGTGATAGTGATGTTACTGGAAGTCCTCTTCAATGCATCCCACCAAG
GGGAGGAAGCCCCTT
GTGAACTCTGAAACTAGAGGTGGCCTTTGGC
mm4_intergenic_A330048O09Rik|Zfp169_chr13_48285474 GGAGAAGCCGTTGTTCCTCTGAGCCTGCCGACTCGGGGAGGAACTCCCATGACAGTGTTTCACAGGCTCCTTGATGAAAT
GGATACAGTTAAAATAGACCCACACAGATTATTATTGCTGGGAAACTGACACTCC
mm4_exon_Lmtk3_chr7_45803873 AGATGAGTCCCCTGATCCCCCTGACCCCCCCGACCCCCTTGGAGCCACATGAGGCAGGAAGCCCCATCGCTGGACAGCCA
CACGTCTTGGAACTGAACTGAACCGAACTCTTTTGGGCCCAGA

Input file for Crispresso

Crispresso, written by Luca Pinello, is a software package to quantify the Cas9-induced mutations on off- or on-targets.

Click here to download an amplicon input file for Crispresso. For each off-target, it includes the off-target name, its PCR amplicon and the guide sequence. Keep a copy of this file.

After sequencing, run CRISPRessoPooled. The tool will map the reads to the amplicons and analyse the mutations:
CRISPRessoPooled -r1 Reads1.fastq.gz -r2 Reads2.fastq.gz -f crisporAmplicons_mN1FkRligAThyqKv3eg3.txt --name MY_EXPERIMENT

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CRISPR/Cas9 Guide Designer for chordate vertebrate ecdysozoans lophotrochozoans protostomes spongi corals plants butterflies metazoans genomes fruitflies insects nematodes mammals.